                         SEQUENCE LISTING

<110>  Antion Biosciences SA
       Geneva University Hospitals
       University of Geneva
       University of Zurich
 
<120>  ADAPTER MOLECULES TO RE-DIRECT CAR T CELLS TO AN ANTIGEN OF 
       INTEREST

<130>  ANBO.P0005WO

<140>  not yet known
<141>  2021-05-27

<150>  US 63/030,653
<151>  2020-05-27

<160>  14    

<170>  PatentIn version 3.5

<210>  1
<211>  1764
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ACE2t DNA sequence (codon optimized)

<400>  1
cagagcacaa ttgaggaaca ggccaagacc ttcctggaca agttcaacca cgaggccgag       60

gacctgttct accagtctag cctggccagc tggaactaca acaccaacat caccgaagag      120

aacgtgcaga acatgaacaa cgccggcgac aagtggagcg ccttcctgaa agagcagagc      180

acactggccc agatgtaccc tctgcaagag atccagaacc tgaccgtgaa gctccagctg      240

caggccctcc agcagaatgg aagctctgtg ctgagcgagg acaagagcaa gcggctgaac      300

accatcctga ataccatgag caccatctac agcaccggca aagtgtgcaa ccccgacaat      360

ccccaagagt gcctgctgct ggaacccggc ctgaatgaga tcatggccaa cagcctggac      420

tacaacgaga gactgtgggc ctgggagtct tggagaagcg aagtgggaaa gcagctgcgg      480

cccctgtacg aggaatacgt ggtgctgaag aacgagatgg ccagagccaa ccactacgag      540

gactacggcg actattggag aggcgactac gaagtgaatg gcgtggacgg ctacgactac      600

agcagaggcc agctgatcga ggacgtggaa cacaccttcg aggaaatcaa gcctctgtac      660

gagcatctgc acgcctacgt gcgggccaag ctgatgaatg cttaccccag ctacatcagc      720

cccatcggct gtctgcctgc tcatctgctg ggagacatgt ggggcagatt ctggaccaac      780

ctgtacagcc tgacagtgcc cttcggccag aaacctaaca tcgacgtgac cgacgctatg      840

gtggatcagg cctgggatgc ccagcggatc ttcaaagagg ccgagaagtt cttcgtgtcc      900

gtgggcctgc ctaatatgac ccaaggcttc tgggagaact ccatgctgac agaccccggc      960

aatgtgcaga aagccgtgtg tcatcctacc gcctgggatc tcggcaaggg cgacttcaga     1020

atcctgatgt gcaccaaagt gacgatggac gacttcctga cagcccacca cgagatgggc     1080

cacatccagt acgatatggc ctacgccgct cagcccttcc tgctgagaaa tggcgccaat     1140

gagggcttcc acgaagccgt gggagagatc atgagcctgt ctgccgccac acctaagcac     1200

ctgaagtcta tcggactgct gagccccgac ttccaagagg acaacgagac agagatcaac     1260

ttcctgctca agcaggccct gaccatcgtg ggcacactgc ccttcaccta catgctggaa     1320

aagtggcggt ggatggtctt taagggcgag atccccaagg accagtggat gaagaaatgg     1380

tgggagatga agcgcgagat cgtgggcgtt gtggaacctg tgcctcacga cgagacatac     1440

tgcgatcctg ccagcctgtt tcacgtgtcc aacgactact ccttcatccg gtactacacc     1500

cggacactgt accagttcca gtttcaagag gctctgtgcc aggccgccaa gcacgaagga     1560

cctctgcaca agtgcgacat cagcaactct acagaggccg gacagaaact gttcaacatg     1620

ctgcggctgg gcaagagcga gccttggaca ctggctctgg aaaatgtcgt gggcgccaag     1680

aatatgaacg tgcggccact gctgaactac ttcgagcccc tgttcacctg gctgaaggac     1740

cagaacaaga acagctttgt cggc                                            1764


<210>  2
<211>  588
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ACE2t protein sequence

<400>  2

Gln Ser Thr Ile Glu Glu Gln Ala Lys Thr Phe Leu Asp Lys Phe Asn 
1               5                   10                  15      


His Glu Ala Glu Asp Leu Phe Tyr Gln Ser Ser Leu Ala Ser Trp Asn 
            20                  25                  30          


Tyr Asn Thr Asn Ile Thr Glu Glu Asn Val Gln Asn Met Asn Asn Ala 
        35                  40                  45              


Gly Asp Lys Trp Ser Ala Phe Leu Lys Glu Gln Ser Thr Leu Ala Gln 
    50                  55                  60                  


Met Tyr Pro Leu Gln Glu Ile Gln Asn Leu Thr Val Lys Leu Gln Leu 
65                  70                  75                  80  


Gln Ala Leu Gln Gln Asn Gly Ser Ser Val Leu Ser Glu Asp Lys Ser 
                85                  90                  95      


Lys Arg Leu Asn Thr Ile Leu Asn Thr Met Ser Thr Ile Tyr Ser Thr 
            100                 105                 110         


Gly Lys Val Cys Asn Pro Asp Asn Pro Gln Glu Cys Leu Leu Leu Glu 
        115                 120                 125             


Pro Gly Leu Asn Glu Ile Met Ala Asn Ser Leu Asp Tyr Asn Glu Arg 
    130                 135                 140                 


Leu Trp Ala Trp Glu Ser Trp Arg Ser Glu Val Gly Lys Gln Leu Arg 
145                 150                 155                 160 


Pro Leu Tyr Glu Glu Tyr Val Val Leu Lys Asn Glu Met Ala Arg Ala 
                165                 170                 175     


Asn His Tyr Glu Asp Tyr Gly Asp Tyr Trp Arg Gly Asp Tyr Glu Val 
            180                 185                 190         


Asn Gly Val Asp Gly Tyr Asp Tyr Ser Arg Gly Gln Leu Ile Glu Asp 
        195                 200                 205             


Val Glu His Thr Phe Glu Glu Ile Lys Pro Leu Tyr Glu His Leu His 
    210                 215                 220                 


Ala Tyr Val Arg Ala Lys Leu Met Asn Ala Tyr Pro Ser Tyr Ile Ser 
225                 230                 235                 240 


Pro Ile Gly Cys Leu Pro Ala His Leu Leu Gly Asp Met Trp Gly Arg 
                245                 250                 255     


Phe Trp Thr Asn Leu Tyr Ser Leu Thr Val Pro Phe Gly Gln Lys Pro 
            260                 265                 270         


Asn Ile Asp Val Thr Asp Ala Met Val Asp Gln Ala Trp Asp Ala Gln 
        275                 280                 285             


Arg Ile Phe Lys Glu Ala Glu Lys Phe Phe Val Ser Val Gly Leu Pro 
    290                 295                 300                 


Asn Met Thr Gln Gly Phe Trp Glu Asn Ser Met Leu Thr Asp Pro Gly 
305                 310                 315                 320 


Asn Val Gln Lys Ala Val Cys His Pro Thr Ala Trp Asp Leu Gly Lys 
                325                 330                 335     


Gly Asp Phe Arg Ile Leu Met Cys Thr Lys Val Thr Met Asp Asp Phe 
            340                 345                 350         


Leu Thr Ala His His Glu Met Gly His Ile Gln Tyr Asp Met Ala Tyr 
        355                 360                 365             


Ala Ala Gln Pro Phe Leu Leu Arg Asn Gly Ala Asn Glu Gly Phe His 
    370                 375                 380                 


Glu Ala Val Gly Glu Ile Met Ser Leu Ser Ala Ala Thr Pro Lys His 
385                 390                 395                 400 


Leu Lys Ser Ile Gly Leu Leu Ser Pro Asp Phe Gln Glu Asp Asn Glu 
                405                 410                 415     


Thr Glu Ile Asn Phe Leu Leu Lys Gln Ala Leu Thr Ile Val Gly Thr 
            420                 425                 430         


Leu Pro Phe Thr Tyr Met Leu Glu Lys Trp Arg Trp Met Val Phe Lys 
        435                 440                 445             


Gly Glu Ile Pro Lys Asp Gln Trp Met Lys Lys Trp Trp Glu Met Lys 
    450                 455                 460                 


Arg Glu Ile Val Gly Val Val Glu Pro Val Pro His Asp Glu Thr Tyr 
465                 470                 475                 480 


Cys Asp Pro Ala Ser Leu Phe His Val Ser Asn Asp Tyr Ser Phe Ile 
                485                 490                 495     


Arg Tyr Tyr Thr Arg Thr Leu Tyr Gln Phe Gln Phe Gln Glu Ala Leu 
            500                 505                 510         


Cys Gln Ala Ala Lys His Glu Gly Pro Leu His Lys Cys Asp Ile Ser 
        515                 520                 525             


Asn Ser Thr Glu Ala Gly Gln Lys Leu Phe Asn Met Leu Arg Leu Gly 
    530                 535                 540                 


Lys Ser Glu Pro Trp Thr Leu Ala Leu Glu Asn Val Val Gly Ala Lys 
545                 550                 555                 560 


Asn Met Asn Val Arg Pro Leu Leu Asn Tyr Phe Glu Pro Leu Phe Thr 
                565                 570                 575     


Trp Leu Lys Asp Gln Asn Lys Asn Ser Phe Val Gly 
            580                 585             


<210>  3
<211>  684
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Modified Fc DNA sequence

<400>  3
tctgataaga cccacacctg tcctccatgt cctgctccag aactgctcgg cggaccttcc       60

gtgttcctgt ttcctccaaa gcctaaggac accctgatga tcagcagaac ccctgaagtg      120

acctgcgtgg tggtggccgt gtctcacgaa gatcccgaag tgaagttcaa ttggtacgtc      180

gacggcgtgg aagtgcacaa cgccaagacc aagcctagag aggaacagta cgccagcacc      240

tacagagtgg tgtccgtgct gactgtgctg caccaggatt ggctgaacgg caaagagtac      300

aagtgcaagg tgtccaacaa ggccctgcca gctcctatcg agaaaaccat cagcaaggcc      360

aagggccagc ctagggaacc ccaggtttac acactgcctc caagcaggga cgagctgacc      420

aagaatcagg tgtccctgac ctgcctggtc aagggctttt acccctccga cattgccgtg      480

gaatgggaga gcaatggcca gcctgagaac aactacaaga ccacacctcc tgtgctggac      540

agcgacggct cattcttcct gtactccaag ctgaccgtgg acaagtccag atggcagcag      600

ggcaacgtgt tcagctgcag cgtgatgcac gaggccctgc acaatcacta cacccagaag      660

tccctgtctc tgagccctgg caaa                                             684


<210>  4
<211>  228
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Modified Fc amino acid sequence

<400>  4

Ser Asp Lys Thr His Thr Cys Pro Pro Cys Pro Ala Pro Glu Leu Leu 
1               5                   10                  15      


Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu 
            20                  25                  30          


Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Ala Val Ser 
        35                  40                  45              


His Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu 
    50                  55                  60                  


Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr Ala Ser Thr 
65                  70                  75                  80  


Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu Asn 
                85                  90                  95      


Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu Pro Ala Pro 
            100                 105                 110         


Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln 
        115                 120                 125             


Val Tyr Thr Leu Pro Pro Ser Arg Asp Glu Leu Thr Lys Asn Gln Val 
    130                 135                 140                 


Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val 
145                 150                 155                 160 


Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro 
                165                 170                 175     


Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr 
            180                 185                 190         


Val Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val 
        195                 200                 205             


Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu 
    210                 215                 220                 


Ser Pro Gly Lys 
225             


<210>  5
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gp120t nucleotide sequence

<400>  5
tcctcaggag gggacccaga aattgtaacg cac                                    33


<210>  6
<211>  11
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  gp120t amino acid sequence

<400>  6

Ser Ser Gly Gly Asp Pro Glu Ile Val Thr His 
1               5                   10      


<210>  7
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  linker sequence

<400>  7

Gly Gly Gly Ser 
1               


<210>  8
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  linker sequence

<400>  8

Ser Ser Gly Gly Gly Gly Ser 
1               5           


<210>  9
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  linker sequence

<400>  9

Ser Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Gly Gly Ser Ser 
1               5                   10                  15  


<210>  10
<211>  18
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  linker sequence

<400>  10

Ser Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Gly Gly Ser Ser Arg 
1               5                   10                  15      


Ser Ser 
        


<210>  11
<211>  32
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CD8a transmembrane domain

<400>  11

Phe Ala Cys Asp Ile Tyr Ile Trp Ala Pro Leu Ala Gly Thr Cys Gly 
1               5                   10                  15      


Val Leu Leu Leu Ser Leu Val Ile Thr Leu Tyr Cys Asn His Arg Asn 
            20                  25                  30          


<210>  12
<211>  43
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CD8a hinge region

<400>  12

Lys Pro Thr Thr Thr Pro Ala Pro Arg Pro Pro Thr Pro Ala Pro Thr 
1               5                   10                  15      


Ile Ala Ser Gln Pro Leu Ser Leu Arg Pro Glu Ala Cys Arg Pro Ala 
            20                  25                  30          


Ala Gly Gly Ala Val His Thr Arg Gly Leu Asp 
        35                  40              


<210>  13
<211>  230
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  hinge-CH2-CH3 region of antibody isotype IgG4

<400>  13

Glu Ser Lys Tyr Gly Pro Pro Cys Pro Pro Cys Pro Ala Pro Glu Phe 
1               5                   10                  15      


Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr 
            20                  25                  30          


Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val 
        35                  40                  45              


Ser Gln Glu Asp Pro Glu Val Gln Phe Asn Trp Tyr Val Asp Gly Val 
    50                  55                  60                  


Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Phe Asn Ser 
65                  70                  75                  80  


Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu 
                85                  90                  95      


Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Gly Leu Pro Ser 
            100                 105                 110         


Ser Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro 
        115                 120                 125             


Gln Val Tyr Thr Leu Pro Pro Ser Gln Glu Glu Met Thr Lys Asn Gln 
    130                 135                 140                 


Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala 
145                 150                 155                 160 


Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr 
                165                 170                 175     


Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Arg Leu 
            180                 185                 190         


Thr Val Asp Lys Ser Arg Trp Gln Glu Gly Asn Val Phe Ser Cys Ser 
        195                 200                 205             


Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser 
    210                 215                 220                 


Leu Ser Leu Gly Lys Met 
225                 230 


<210>  14
<211>  112
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CD3zeta intracellular domain

<400>  14

Arg Val Lys Phe Ser Arg Ser Ala Asp Ala Pro Ala Tyr Gln Gln Gly 
1               5                   10                  15      


Gln Asn Gln Leu Tyr Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr 
            20                  25                  30          


Asp Val Leu Asp Lys Arg Arg Gly Arg Asp Pro Glu Met Gly Gly Lys 
        35                  40                  45              


Pro Arg Arg Lys Asn Pro Gln Glu Gly Leu Tyr Asn Glu Leu Gln Lys 
    50                  55                  60                  


Asp Lys Met Ala Glu Ala Tyr Ser Glu Ile Gly Met Lys Gly Glu Arg 
65                  70                  75                  80  


Arg Arg Gly Lys Gly His Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala 
                85                  90                  95      


Thr Lys Asp Thr Tyr Asp Ala Leu His Met Gln Ala Leu Pro Pro Arg 
            100                 105                 110         


