                         SEQUENCE LISTING

<110>  Cambridge Enterprise Limited
 
<120>  OLIGONUCLEOTIDE ADAPTERS AND METHOD

<130>  |P10681GBWO|

<150>  GB2007970.3
<151>  2020-05-28

<160>  36    

<170>  PatentIn version 3.5

<210>  1
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  (Illumina i7 flow cell binding site for immobilisation)

<400>  1
atctcgtatg ccgtcttctg cttg                                              24


<210>  2
<211>  29
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  (Illumina i5 flow cell binding site for immobilisation)

<400>  2
aatgatacgg cgaccaccga gatctacac                                         29


<210>  3
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  (binding site for at least one oligonucleotide primer - Illumina 
       i7 adapter/primer) (33nt)

<400>  3
gatcggaaga gcacacgtct gaactccagt cac                                    33


<210>  4
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  (binding site for at least one oligonucleotide primer - Illumina 
       i5 adapter/primer) (33nt)

<400>  4
acactctttc cctacacgac gctcttccga tct                                    33


<210>  5
<211>  34
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  (binding site for at least one oligonucleotide primer - Illumina 
       i7 adapter/primer) (34nt including additional 5' T)

<400>  5
agatcggaag agcacacgtc tgaactccag tcac                                   34


<210>  6
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  (binding site for at least one oligonucleotide primer - Illumina 
       i7 adapter/primer) (33nt) (reverse complement of SEQ ID NO: 3)

<400>  6
gtgactggag ttcagacgtg tgctcttccg atc                                    33


<210>  7
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  (binding site for at least one oligonucleotide primer - Illumina 
       i5 adapter/primer) (33nt) (reverse complement of SEQ ID NO: 4)

<400>  7
agatcggaag agcgtcgtgt agggaaagag tgt                                    33


<210>  8
<211>  34
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  (binding site for at least one oligonucleotide primer - Illumina 
       i7 adapter/primer) (34nt including additional 5' T) (reverse 
       complement of SEQ ID NO: 5)

<400>  8
gtgactggag ttcagacgtg tgctcttccg atct                                   34


<210>  9
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  (ONT forward/left end (i5) adapter/primer i.e. binding site for 
       at least one oligonucleotide primer - first adapter)

<400>  9
cttctatctc gctgtccgtt ca                                                22


<210>  10
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  (ONT reverse/right end (i5) adapter/primer i.e. binding site for 
       at least one oligonucleotide primer - second adapter)

<400>  10
tttctgttgg tgctgatatt gc                                                22


<210>  11
<211>  38
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer


<220>
<221>  N16
<222>  (16)..(16)
<223>  barcode (Nx)

<220>
<221>  misc_feature
<222>  (16)..(16)
<223>  n is a, c, g, or t

<400>  11
atcgcctacc gtgacnactt gcctgtcgct ctatcttc                               38


<210>  12
<211>  38
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer


<220>
<221>  N16
<222>  (16)..(16)
<223>  barcode (Nx)

<220>
<221>  misc_feature
<222>  (16)..(16)
<223>  n is a, c, g, or t

<400>  12
atcgcctacc gtgacntttc tgttggtgct gatattgc                               38


<210>  13
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  mutREAD-i5-upper_1


<220>
<221>  misc_feature
<222>  (15)..(18)
<223>  Unique Molecular Identifier

<400>  13
cgctcttccg atcthnnnat gagcgatgca                                        30


<210>  14
<211>  45
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  mutREAD-i5-lower_1


<220>
<221>  misc_feature
<222>  (9)..(12)
<223>  Unique Molecular Identifier

<400>  14
tcgctcatnn ndagatcgga agagcgtcgt gtagggaaag agtgt                       45


<210>  15
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  mutREAD-i5-upper_2


<220>
<221>  misc_feature
<222>  (15)..(18)
<223>  Unique Molecular Identifier

<400>  15
cgctcttccg atcthnnngc ctagcgtgca                                        30


<210>  16
<211>  45
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  mutREAD-i5-lower_2


<220>
<221>  misc_feature
<222>  (9)..(12)
<223>  Unique Molecular Identifier

<400>  16
cgctaggcnn ndagatcgga agagcgtcgt gtagggaaag agtgt                       45


<210>  17
<211>  46
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  mutREAD-i7-upper_1


<220>
<221>  misc_feature
<222>  (35)..(38)
<223>  Unique Molecular Identifier

<400>  17
gtgactggag ttcagacgtg tgctcttccg atcthnnncg tgtacc                      46


<210>  18
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  mutREAD-i7-lower_1


<220>
<221>  misc_feature
<222>  (13)..(16)
<223>  Unique Molecular Identifier

<400>  18
aattggtaca cgnnndagat cggaagagca                                        30


<210>  19
<211>  46
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  mutREAD-i7-upper_2


<220>
<221>  misc_feature
<222>  (35)..(38)
<223>  Unique Molecular Identifier

<400>  19
gtgactggag ttcagacgtg tgctcttccg atcthnnngc acatgt                      46


<210>  20
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  mutREAD-i7-lower_2


<220>
<221>  misc_feature
<222>  (13)..(16)
<223>  Unique Molecular Identifier

<400>  20
aattacatgt gcnnndagat cggaagagca                                        30


<210>  21
<211>  58
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  mutREAD-i501


<220>
<221>  misc_feature
<222>  (57)..(58)
<223>  phosphorothioate bond

<400>  21
aatgatacgg cgaccaccga gatctacact atagcctaca ctctttccct acacgacg         58


<210>  22
<211>  58
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  mutREAD-i502


<220>
<221>  misc_feature
<222>  (57)..(58)
<223>  phosphorothioate bond

<400>  22
aatgatacgg cgaccaccga gatctacaca tagaggcaca ctctttccct acacgacg         58


<210>  23
<211>  56
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  mutREAD-i701


<220>
<221>  misc_feature
<222>  (57)..(58)
<223>  phosphorothioate bond

<400>  23
caagcagaag acggcatacg agatcgagta atgtgactgg agttcagacg tgtgct           56


<210>  24
<211>  56
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  mutREAD-i702


<220>
<221>  misc_feature
<222>  (55)..(56)
<223>  phosphorothioate bond

<400>  24
caagcagaag acggcatacg agattctccg gagtgactgg agttcagacg tgtgct           56


<210>  25
<211>  12
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  MutRead-i5-upper_1

<400>  25
gcatgggatg ca                                                           12


<210>  26
<211>  45
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  MutRead-i5-lower_1


<220>
<221>  misc_feature
<222>  (9)..(12)
<223>  Unique Molecular Identifier

<220>
<221>  misc_feature
<222>  (39)..(40)
<223>  phosphorothioate bond

<220>
<221>  misc_feature
<222>  (40)..(41)
<223>  phosphorothioate bond

<220>
<221>  misc_feature
<222>  (41)..(42)
<223>  phosphorothioate bond

<220>
<221>  misc_feature
<222>  (42)..(43)
<223>  phosphorothioate bond

<220>
<221>  misc_feature
<222>  (43)..(44)
<223>  phosphorothioate bond

<220>
<221>  misc_feature
<222>  (44)..(45)
<223>  phosphorothioate bond

<400>  26
tcccatgcnn nnagatcgga agagcgtcgt gtagggaaag agtgt                       45


<210>  27
<211>  46
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  MutRead-i7-upper_1


<220>
<221>  misc_feature
<222>  (1)..(2)
<223>  phosphorothioate bond

<220>
<221>  misc_feature
<222>  (2)..(3)
<223>  phosphorothioate bond

<220>
<221>  misc_feature
<222>  (3)..(4)
<223>  phosphorothioate bond

<220>
<221>  misc_feature
<222>  (4)..(5)
<223>  phosphorothioate bond

<220>
<221>  misc_feature
<222>  (5)..(6)
<223>  phosphorothioate bond

<220>
<221>  misc_feature
<222>  (6)..(7)
<223>  phosphorothioate bond

<220>
<221>  misc_feature
<222>  (35)..(38)
<223>  unique molecular identifier

<400>  27
gtgactggag ttcagacgtg tgctcttccg atctnnnnga cgtacc                      46


<210>  28
<211>  12
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  MutRead-i7-lower_1

<400>  28
aattggtacg tc                                                           12


<210>  29
<211>  58
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  MutRead-i501


<220>
<221>  misc_feature
<222>  (57)..(58)
<223>  phosphorothioate bond

<400>  29
aatgatacgg cgaccaccga gatctacact atagcctaca ctctttccct acacgacg         58


<210>  30
<211>  58
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  MutRead-i502


<220>
<221>  misc_feature
<222>  (57)..(58)
<223>  phosphorothioate bond

<400>  30
aatgatacgg cgaccaccga gatctacaca tagaggcaca ctctttccct acacgacg         58


<210>  31
<211>  58
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  MutRead-i503

<400>  31
aatgatacgg cgaccaccga gatctacacc ctatcctaca ctctttccct acacgacg         58


<210>  32
<211>  58
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  MutRead-i504


<220>
<221>  misc_feature
<222>  (57)..(58)
<223>  phosphorothioate bond

<400>  32
aatgatacgg cgaccaccga gatctacacg gctctgaaca ctctttccct acacgacg         58


<210>  33
<211>  56
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  MutRead-i701


<220>
<221>  misc_feature
<222>  (55)..(56)
<223>  phosphorothioate bond

<400>  33
caagcagaag acggcatacg agatcgagta atgtgactgg agttcagacg tgtgct           56


<210>  34
<211>  56
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  MutRead-i702


<220>
<221>  misc_feature
<222>  (55)..(56)
<223>  phosphorothioate bond

<400>  34
caagcagaag acggcatacg agattctccg gagtgactgg agttcagacg tgtgct           56


<210>  35
<211>  56
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  MutRead-i703


<220>
<221>  misc_feature
<222>  (55)..(56)
<223>  phosphorothioate bond

<400>  35
caagcagaag acggcatacg agataatgag cggtgactgg agttcagacg tgtgct           56


<210>  36
<211>  56
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  MutRead-i704


<220>
<221>  misc_feature
<222>  (55)..(56)
<223>  phosphorothioate bond

<400>  36
caagcagaag acggcatacg agatggaatc tcgtgactgg agttcagacg tgtgct           56


