                         SEQUENCE LISTING

<110>  տԵҽҩƼ޹˾
 
<120>  rhFGF21ںϵסĶᡢＰ;

<160>  13    

<170>  PatentIn version 3.5

<210>  1
<211>  181
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  rhFGF21

<400>  1

His Pro Ile Pro Asp Ser Ser Pro Leu Leu Gln Phe Gly Gly Gln Val 
1               5                   10                  15      


Arg Gln Arg Tyr Leu Tyr Thr Asp Asp Ala Gln Gln Thr Glu Ala His 
            20                  25                  30          


Leu Glu Ile Arg Glu Asp Gly Thr Val Gly Gly Ala Ala Asp Gln Ser 
        35                  40                  45              


Pro Glu Ser Leu Leu Gln Leu Lys Ala Leu Lys Pro Gly Val Ile Gln 
    50                  55                  60                  


Ile Leu Gly Val Lys Thr Ser Arg Phe Leu Cys Gln Arg Pro Asp Gly 
65                  70                  75                  80  


Ala Leu Tyr Gly Ser Leu His Phe Asp Pro Glu Ala Cys Ser Phe Arg 
                85                  90                  95      


Glu Leu Leu Leu Glu Asp Gly Tyr Asn Val Tyr Gln Ser Glu Ala His 
            100                 105                 110         


Gly Leu Pro Leu His Leu Pro Gly Asn Lys Ser Pro His Arg Asp Pro 
        115                 120                 125             


Ala Pro Arg Gly Pro Ala Arg Phe Leu Pro Leu Pro Gly Leu Pro Pro 
    130                 135                 140                 


Ala Leu Pro Glu Pro Pro Gly Ile Leu Ala Pro Gln Pro Pro Asp Val 
145                 150                 155                 160 


Gly Ser Ser Asp Pro Leu Ser Met Val Gly Pro Ser Gln Gly Arg Ser 
                165                 170                 175     


Pro Ser Tyr Ala Ser 
            180     


<210>  2
<211>  385
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  rhFGF21-(VPGXG)40 fusion protein

<400>  2

His Pro Ile Pro Asp Ser Ser Pro Leu Leu Gln Phe Gly Gly Gln Val 
1               5                   10                  15      


Arg Gln Arg Tyr Leu Tyr Thr Asp Asp Ala Gln Gln Thr Glu Ala His 
            20                  25                  30          


Leu Glu Ile Arg Glu Asp Gly Thr Val Gly Gly Ala Ala Asp Gln Ser 
        35                  40                  45              


Pro Glu Ser Leu Leu Gln Leu Lys Ala Leu Lys Pro Gly Val Ile Gln 
    50                  55                  60                  


Ile Leu Gly Val Lys Thr Ser Arg Phe Leu Cys Gln Arg Pro Asp Gly 
65                  70                  75                  80  


Ala Leu Tyr Gly Ser Leu His Phe Asp Pro Glu Ala Cys Ser Phe Arg 
                85                  90                  95      


Glu Leu Leu Leu Glu Asp Gly Tyr Asn Val Tyr Gln Ser Glu Ala His 
            100                 105                 110         


Gly Leu Pro Leu His Leu Pro Gly Asn Lys Ser Pro His Arg Asp Pro 
        115                 120                 125             


Ala Pro Arg Gly Pro Ala Arg Phe Leu Pro Leu Pro Gly Leu Pro Pro 
    130                 135                 140                 


Ala Leu Pro Glu Pro Pro Gly Ile Leu Ala Pro Gln Pro Pro Asp Val 
145                 150                 155                 160 


Gly Ser Ser Asp Pro Leu Ser Met Val Gly Pro Ser Gln Gly Arg Ser 
                165                 170                 175     


Pro Ser Tyr Ala Ser Arg Ser Val Pro Gly Ala Gly Val Pro Gly Ala 
            180                 185                 190         


Gly Val Pro Gly Ala Gly Val Pro Gly Ala Gly Val Pro Gly Ala Gly 
        195                 200                 205             


Val Pro Gly Ala Gly Val Pro Gly Ala Gly Val Pro Gly Ala Gly Val 
    210                 215                 220                 


Pro Gly Val Gly Val Pro Gly Val Gly Val Pro Gly Val Gly Val Pro 
225                 230                 235                 240 


Gly Val Gly Val Pro Gly Val Gly Val Pro Gly Val Gly Val Pro Gly 
                245                 250                 255     


Val Gly Val Pro Gly Val Gly Val Pro Gly Val Gly Val Pro Gly Val 
            260                 265                 270         


Gly Val Pro Gly Val Gly Val Pro Gly Val Gly Gly Ser Val Pro Gly 
        275                 280                 285             


Ala Gly Val Pro Gly Ala Gly Val Pro Gly Ala Gly Val Pro Gly Ala 
    290                 295                 300                 


Gly Val Pro Gly Ala Gly Val Pro Gly Ala Gly Val Pro Gly Ala Gly 
305                 310                 315                 320 


Val Pro Gly Ala Gly Val Pro Gly Val Gly Val Pro Gly Val Gly Val 
                325                 330                 335     


Pro Gly Val Gly Val Pro Gly Val Gly Val Pro Gly Val Gly Val Pro 
            340                 345                 350         


Gly Val Gly Val Pro Gly Val Gly Val Pro Gly Val Gly Val Pro Gly 
        355                 360                 365             


Val Gly Val Pro Gly Val Gly Val Pro Gly Val Gly Val Pro Gly Val 
    370                 375                 380                 


Gly 
385 


<210>  3
<211>  543
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  nucleotide sequence for encoding rhFGF21

<400>  3
catcctattc ctgacagctc acccctgtta cagtttggcg gtcaggttcg tcaacgctat       60

ttatacaccg acgacgcaca gcaaaccgaa gcacacctgg aaattcgtga agacggcacc      120

gttggtggtg cagcagatca atccccggaa tctctgctgc agttaaaagc attaaaaccg      180

ggcgttatac agattttagg tgtcaaaacc tcccgttttc tgtgtcagcg gccggacggt      240

gcgctttatg gtagcttaca ctttgatccg gaagcctgta gttttcgtga gctgctgctg      300

gaggacggtt acaatgttta tcaatcagaa gcacatggtc tgccgttaca tctgccgggt      360

aataagagcc cgcatcgcga tccggcaccg cgtggtccag ccagatttct gcctctgccg      420

ggactgccgc cggcacttcc tgaaccgcct ggtattctgg caccgcagcc gcctgatgtt      480

ggttcatctg atcctctgtc aatggttgga ccgagccagg gacgttctcc ctcatacgct      540

tct                                                                    543


<210>  4
<211>  6
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  nucleotide sequence for encoding linker

<400>  4
agatct                                                                  6


<210>  5
<211>  6
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  nucleotide sequence for encoding linker

<400>  5
ggatcc                                                                  6


<210>  6
<211>  1158
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  nucleotide sequence for encoding rhFGF21-(VPGXG)40 fusion protein

<400>  6
catcctattc ctgacagctc acccctgtta cagtttggcg gtcaggttcg tcaacgctat       60

ttatacaccg acgacgcaca gcaaaccgaa gcacacctgg aaattcgtga agacggcacc      120

gttggtggtg cagcagatca atccccggaa tctctgctgc agttaaaagc attaaaaccg      180

ggcgttatac agattttagg tgtcaaaacc tcccgttttc tgtgtcagcg gccggacggt      240

gcgctttatg gtagcttaca ctttgatccg gaagcctgta gttttcgtga gctgctgctg      300

gaggacggtt acaatgttta tcaatcagaa gcacatggtc tgccgttaca tctgccgggt      360

aataagagcc cgcatcgcga tccggcaccg cgtggtccag ccagatttct gcctctgccg      420

ggactgccgc cggcacttcc tgaaccgcct ggtattctgg caccgcagcc gcctgatgtt      480

ggttcatctg atcctctgtc aatggttgga ccgagccagg gacgttctcc ctcatacgct      540

tctagatctg ttccgggtgc aggtgttccg ggtgccggtg ttccgggggc aggtgttcct      600

ggtgcgggtg ttccgggcgc gggtgttcct ggagcgggtg ttccaggtgc gggtgtgccg      660

ggtgcgggtg tacctggtgt tggtgttccg ggagttggtg ttcctggggt tggtgttcca      720

ggggttggtg tgccgggggt tggtgtcccg ggtgttggtg tgcctggtgt tggggttccg      780

ggtgttgggg tgccgggtgt tggcgttccg ggtgtgggtg ttccgggtgt cggtgttccg      840

ggtgtaggtg gatctgttcc gggtgcaggt gttccgggtg ccggtgttcc gggggcaggt      900

gttcctggtg cgggtgttcc gggcgcgggt gttcctggag cgggtgttcc aggtgcgggt      960

gtgccgggtg cgggtgtacc tggtgttggt gttccgggag ttggtgttcc tggggttggt     1020

gttccagggg ttggtgtgcc gggggttggt gtcccgggtg ttggtgtgcc tggtgttggg     1080

gttccgggtg ttggggtgcc gggtgttggc gttccgggtg tgggtgttcc gggtgtcggt     1140

gttccgggtg taggttga                                                   1158


<210>  7
<211>  303
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  nucleotide sequence for encoding (VPGXG)20

<400>  7
gttccgggtg caggtgttcc gggtgccggt gttccggggg caggtgttcc tggtgcgggt       60

gttccgggcg cgggtgttcc tggagcgggt gttccaggtg cgggtgtgcc gggtgcgggt      120

gtacctggtg ttggtgttcc gggagttggt gttcctgggg ttggtgttcc aggggttggt      180

gtgccggggg ttggtgtccc gggtgttggt gtgcctggtg ttggggttcc gggtgttggg      240

gtgccgggtg ttggcgttcc gggtgtgggt gttccgggtg tcggtgttcc gggtgtaggt      300

tga                                                                    303


<210>  8
<211>  559
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gene for encoding rhFGF21 with restriction site

<400>  8
ggtctcaggt catcctattc ctgacagctc acccctgtta cagtttggcg gtcaggttcg       60

tcaacgctat ttatacaccg acgacgcaca gcaaaccgaa gcacacctgg aaattcgtga      120

agacggcacc gttggtggtg cagcagatca atccccggaa tctctgctgc agttaaaagc      180

attaaaaccg ggcgttatac agattttagg tgtcaaaacc tcccgttttc tgtgtcagcg      240

gccggacggt gcgctttatg gtagcttaca ctttgatccg gaagcctgta gttttcgtga      300

gctgctgctg gaggacggtt acaatgttta tcaatcagaa gcacatggtc tgccgttaca      360

tctgccgggt aataagagcc cgcatcgcga tccggcaccg cgtggtccag ccagatttct      420

gcctctgccg ggactgccgc cggcacttcc tgaaccgcct ggtattctgg caccgcagcc      480

gcctgatgtt ggttcatctg atcctctgtc aatggttgga ccgagccagg gacgttctcc      540

ctcatacgct tctagatct                                                   559


<210>  9
<211>  312
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gene for encoding linker-(VPGXG)20 

<400>  9
agatctgttc cgggtgcagg tgttccgggt gccggtgttc cgggggcagg tgttcctggt       60

gcgggtgttc cgggcgcggg tgttcctgga gcgggtgttc caggtgcggg tgtgccgggt      120

gcgggtgtac ctggtgttgg tgttccggga gttggtgttc ctggggttgg tgttccaggg      180

gttggtgtgc cgggggttgg tgtcccgggt gttggtgtgc ctggtgttgg ggttccgggt      240

gttggggtgc cgggtgttgg cgttccgggt gtgggtgttc cgggtgtcgg tgttccgggt      300

gtaggtggat cc                                                          312


<210>  10
<211>  10
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  restriction site of BsaI

<400>  10
ggtctcaggt                                                              10


<210>  11
<211>  29
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer P1

<400>  11
ggtctcaggt catcctattc ctgacagct                                         29


<210>  12
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer P2

<400>  12
ggatccacct acacccggaa ca                                                22


<210>  13
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer P3

<400>  13
ggatcctcaa cctacacccg gaaca                                             25


