﻿                         序列表

<110>  BEIJING VDJBIO CO., LTD.

<120>  An Interleukin-1 Receptor Antagonist and a Fusion Protein Containing the Same

<130>  RYP2010695.3

<140>  
<141>  

<160>  24

<170>  SIPOSequenceListing 1.0

<210>  1
<211>  152
<212>  PRT
<213>  Wild Type IL-1RN Protein Sequence 
<400>  1
Arg Pro Ser Gly Arg Lys Ser Ser Lys Met Gln Ala Phe Arg Ile Trp 
1               5                   10                  15      
Asp Val Asn Gln Lys Thr Phe Tyr Leu Arg Asn Asn Gln Leu Val Ala 
            20                  25                  30          
Gly Tyr Leu Gln Gly Pro Asn Val Asn Leu Glu Glu Lys Ile Asp Val 
        35                  40                  45              
Val Pro Ile Glu Pro His Ala Leu Phe Leu Gly Ile His Gly Gly Lys 
    50                  55                  60                  
Met Cys Leu Ser Cys Val Lys Ser Gly Asp Glu Thr Arg Leu Gln Leu 
65                  70                  75                  80  
Glu Ala Val Asn Ile Thr Asp Leu Ser Glu Asn Arg Lys Gln Asp Lys 
                85                  90                  95      
Arg Phe Ala Phe Ile Arg Ser Asp Ser Gly Pro Thr Thr Ser Phe Glu 
            100                 105                 110         
Ser Ala Ala Cys Pro Gly Trp Phe Leu Cys Thr Ala Met Glu Ala Asp 
        115                 120                 125             
Gln Pro Val Ser Leu Thr Asn Met Pro Asp Glu Gly Val Met Val Thr 
    130                 135                 140                 
Lys Phe Tyr Phe Gln Glu Asp Glu 
145                 150         

<210>  2
<211>  456
<212>  DNA
<213>  Wild Type IL-1RN Nucleotide Sequence

<400>  2
cgaccctctg ggagaaaatc cagcaagatg caagccttca gaatctggga tgttaaccag        60
aagaccttct atctgaggaa caaccaacta gttgctggat acttgcaagg accaaatgtc       120
aatttagaag aaaagataga tgtggtaccc attgagcctc atgctctgtt cttgggaatc       180
catggaggga agatgtgcct gtcctgtgtc aagtctggtg atgagaccag actccagctg       240
gaggcagtta acatcactga cctgagcgag aacagaaagc aggacaagcg cttcgccttc       300
atccgctcag acagcggccc caccaccagt tttgagtctg ccgcctgccc cggttggttc       360
ctctgcacag cgatggaagc tgaccagccc gtcagcctca ccaatatgcc tgacgaaggc       420
gtcatggtca ccaaattcta cttccaggag gacgag                                 456

<210>  3
<211>  152
<212>  PRT
<213>  IL-1RN D74N Protein Sequence

<400>  3
Arg Pro Ser Gly Arg Lys Ser Ser Lys Met Gln Ala Phe Arg Ile Trp 
1               5                   10                  15      
Asp Val Asn Gln Lys Thr Phe Tyr Leu Arg Asn Asn Gln Leu Val Ala 
            20                  25                  30          
Gly Tyr Leu Gln Gly Pro Asn Val Asn Leu Glu Glu Lys Ile Asp Val 
        35                  40                  45              
Val Pro Ile Glu Pro His Ala Leu Phe Leu Gly Ile His Gly Gly Lys 
    50                  55                  60                  
Met Cys Leu Ser Cys Val Lys Ser Gly Asn Glu Thr Arg Leu Gln Leu 
65                  70                  75                  80  
Glu Ala Val Asn Ile Thr Asp Leu Ser Glu Asn Arg Lys Gln Asp Lys 
                85                  90                  95      
Arg Phe Ala Phe Ile Arg Ser Asp Ser Gly Pro Thr Thr Ser Phe Glu 
            100                 105                 110         
Ser Ala Ala Cys Pro Gly Trp Phe Leu Cys Thr Ala Met Glu Ala Asp 
        115                 120                 125             
Gln Pro Val Ser Leu Thr Asn Met Pro Asp Glu Gly Val Met Val Thr 
    130                 135                 140                 
Lys Phe Tyr Phe Gln Glu Asp Glu 
145                 150         

<210>  4
<211>  456
<212>  DNA
<213>  IL-1RN D74N Nucleotide Sequence

<400>  4
cgaccctctg ggagaaaatc cagcaagatg caagccttca gaatctggga tgttaaccag        60
aagaccttct atctgaggaa caaccaacta gttgctggat acttgcaagg accaaatgtc       120
aatttagaag aaaagataga tgtggtaccc attgagcctc atgctctgtt cttgggaatc       180
catggaggga agatgtgcct gtcctgtgtc aagtctggta atgagaccag actccagctg       240
gaggcagtta acatcactga cctgagcgag aacagaaagc aggacaagcg cttcgccttc       300
atccgctcag acagcggccc caccaccagt tttgagtctg ccgcctgccc cggttggttc       360
ctctgcacag cgatggaagc tgaccagccc gtcagcctca ccaatatgcc tgacgaaggc       420
gtcatggtca ccaaattcta cttccaggag gacgag                                 456

<210>  5
<211>  152
<212>  PRT
<213>  IL-1RN R5T Protein Sequence

<400>  5
Arg Pro Ser Gly Thr Lys Ser Ser Lys Met Gln Ala Phe Arg Ile Trp 
1               5                   10                  15      
Asp Val Asn Gln Lys Thr Phe Tyr Leu Arg Asn Asn Gln Leu Val Ala 
            20                  25                  30          
Gly Tyr Leu Gln Gly Pro Asn Val Asn Leu Glu Glu Lys Ile Asp Val 
        35                  40                  45              
Val Pro Ile Glu Pro His Ala Leu Phe Leu Gly Ile His Gly Gly Lys 
    50                  55                  60                  
Met Cys Leu Ser Cys Val Lys Ser Gly Asp Glu Thr Arg Leu Gln Leu 
65                  70                  75                  80  
Glu Ala Val Asn Ile Thr Asp Leu Ser Glu Asn Arg Lys Gln Asp Lys 
                85                  90                  95      
Arg Phe Ala Phe Ile Arg Ser Asp Ser Gly Pro Thr Thr Ser Phe Glu 
            100                 105                 110         
Ser Ala Ala Cys Pro Gly Trp Phe Leu Cys Thr Ala Met Glu Ala Asp 
        115                 120                 125             
Gln Pro Val Ser Leu Thr Asn Met Pro Asp Glu Gly Val Met Val Thr 
    130                 135                 140                 
Lys Phe Tyr Phe Gln Glu Asp Glu 
145                 150         

<210>  6
<211>  456
<212>  DNA
<213>  IL-1RN R5T Nucleotide Sequence

<400>  6
cgaccctctg ggacaaaatc cagcaagatg caagccttca gaatctggga tgttaaccag        60
aagaccttct atctgaggaa caaccaacta gttgctggat acttgcaagg accaaatgtc       120
aatttagaag aaaagataga tgtggtaccc attgagcctc atgctctgtt cttgggaatc       180
catggaggga agatgtgcct gtcctgtgtc aagtctggtg atgagaccag actccagctg       240
gaggcagtta acatcactga cctgagcgag aacagaaagc aggacaagcg cttcgccttc       300
atccgctcag acagcggccc caccaccagt tttgagtctg ccgcctgccc cggttggttc       360
ctctgcacag cgatggaagc tgaccagccc gtcagcctca ccaatatgcc tgacgaaggc       420
gtcatggtca ccaaattcta cttccaggag gacgag                                 456

<210>  7
<211>  152
<212>  PRT
<213>  IL-1RN R14T Protein Sequence

<400>  7
Arg Pro Ser Gly Arg Lys Ser Ser Lys Met Gln Ala Phe Thr Ile Trp 
1               5                   10                  15      
Asp Val Asn Gln Lys Thr Phe Tyr Leu Arg Asn Asn Gln Leu Val Ala 
            20                  25                  30          
Gly Tyr Leu Gln Gly Pro Asn Val Asn Leu Glu Glu Lys Ile Asp Val 
        35                  40                  45              
Val Pro Ile Glu Pro His Ala Leu Phe Leu Gly Ile His Gly Gly Lys 
    50                  55                  60                  
Met Cys Leu Ser Cys Val Lys Ser Gly Asp Glu Thr Arg Leu Gln Leu 
65                  70                  75                  80  
Glu Ala Val Asn Ile Thr Asp Leu Ser Glu Asn Arg Lys Gln Asp Lys 
                85                  90                  95      
Arg Phe Ala Phe Ile Arg Ser Asp Ser Gly Pro Thr Thr Ser Phe Glu 
            100                 105                 110         
Ser Ala Ala Cys Pro Gly Trp Phe Leu Cys Thr Ala Met Glu Ala Asp 
        115                 120                 125             
Gln Pro Val Ser Leu Thr Asn Met Pro Asp Glu Gly Val Met Val Thr 
    130                 135                 140                 
Lys Phe Tyr Phe Gln Glu Asp Glu 
145                 150         

<210>  8
<211>  456
<212>  DNA
<213>  IL-1RN R14T Nucleotide Sequence

<400>  8
cgaccctctg ggagaaaatc cagcaagatg caagccttca caatctggga tgttaaccag        60
aagaccttct atctgaggaa caaccaacta gttgctggat acttgcaagg accaaatgtc       120
aatttagaag aaaagataga tgtggtaccc attgagcctc atgctctgtt cttgggaatc       180
catggaggga agatgtgcct gtcctgtgtc aagtctggtg atgagaccag actccagctg       240
gaggcagtta acatcactga cctgagcgag aacagaaagc aggacaagcg cttcgccttc       300
atccgctcag acagcggccc caccaccagt tttgagtctg ccgcctgccc cggttggttc       360
ctctgcacag cgatggaagc tgaccagccc gtcagcctca ccaatatgcc tgacgaaggc       420
gtcatggtca ccaaattcta cttccaggag gacgag                                 456

<210>  9
<211>  152
<212>  PRT
<213>  IL-1RN R5T+R14T Protein Sequence

<400>  9
Arg Pro Ser Gly Thr Lys Ser Ser Lys Met Gln Ala Phe Thr Ile Trp 
1               5                   10                  15      
Asp Val Asn Gln Lys Thr Phe Tyr Leu Arg Asn Asn Gln Leu Val Ala 
            20                  25                  30          
Gly Tyr Leu Gln Gly Pro Asn Val Asn Leu Glu Glu Lys Ile Asp Val 
        35                  40                  45              
Val Pro Ile Glu Pro His Ala Leu Phe Leu Gly Ile His Gly Gly Lys 
    50                  55                  60                  
Met Cys Leu Ser Cys Val Lys Ser Gly Asp Glu Thr Arg Leu Gln Leu 
65                  70                  75                  80  
Glu Ala Val Asn Ile Thr Asp Leu Ser Glu Asn Arg Lys Gln Asp Lys 
                85                  90                  95      
Arg Phe Ala Phe Ile Arg Ser Asp Ser Gly Pro Thr Thr Ser Phe Glu 
            100                 105                 110         
Ser Ala Ala Cys Pro Gly Trp Phe Leu Cys Thr Ala Met Glu Ala Asp 
        115                 120                 125             
Gln Pro Val Ser Leu Thr Asn Met Pro Asp Glu Gly Val Met Val Thr 
    130                 135                 140                 
Lys Phe Tyr Phe Gln Glu Asp Glu 
145                 150         

<210>  10
<211>  456
<212>  DNA
<213>  IL-1RN R5T+R14T Nucleotide Sequence

<400>  10
cgaccctctg ggacaaaatc cagcaagatg caagccttca caatctggga tgttaaccag        60
aagaccttct atctgaggaa caaccaacta gttgctggat acttgcaagg accaaatgtc       120
aatttagaag aaaagataga tgtggtaccc attgagcctc atgctctgtt cttgggaatc       180
catggaggga agatgtgcct gtcctgtgtc aagtctggtg atgagaccag actccagctg       240
gaggcagtta acatcactga cctgagcgag aacagaaagc aggacaagcg cttcgccttc       300
atccgctcag acagcggccc caccaccagt tttgagtctg ccgcctgccc cggttggttc       360
ctctgcacag cgatggaagc tgaccagccc gtcagcctca ccaatatgcc tgacgaaggc       420
gtcatggtca ccaaattcta cttccaggag gacgag                                 456

<210>  11
<211>  152
<212>  PRT
<213>  IL-1RN R5T+D74N Protein Sequence

<400>  11
Arg Pro Ser Gly Thr Lys Ser Ser Lys Met Gln Ala Phe Arg Ile Trp 
1               5                   10                  15      
Asp Val Asn Gln Lys Thr Phe Tyr Leu Arg Asn Asn Gln Leu Val Ala 
            20                  25                  30          
Gly Tyr Leu Gln Gly Pro Asn Val Asn Leu Glu Glu Lys Ile Asp Val 
        35                  40                  45              
Val Pro Ile Glu Pro His Ala Leu Phe Leu Gly Ile His Gly Gly Lys 
    50                  55                  60                  
Met Cys Leu Ser Cys Val Lys Ser Gly Asn Glu Thr Arg Leu Gln Leu 
65                  70                  75                  80  
Glu Ala Val Asn Ile Thr Asp Leu Ser Glu Asn Arg Lys Gln Asp Lys 
                85                  90                  95      
Arg Phe Ala Phe Ile Arg Ser Asp Ser Gly Pro Thr Thr Ser Phe Glu 
            100                 105                 110         
Ser Ala Ala Cys Pro Gly Trp Phe Leu Cys Thr Ala Met Glu Ala Asp 
        115                 120                 125             
Gln Pro Val Ser Leu Thr Asn Met Pro Asp Glu Gly Val Met Val Thr 
    130                 135                 140                 
Lys Phe Tyr Phe Gln Glu Asp Glu 
145                 150         

<210>  12
<211>  456
<212>  DNA
<213>  IL-1RN R5T+D74N Nucleotide Sequence

<400>  12
cgaccctctg ggacaaaatc cagcaagatg caagccttca gaatctggga tgttaaccag        60
aagaccttct atctgaggaa caaccaacta gttgctggat acttgcaagg accaaatgtc       120
aatttagaag aaaagataga tgtggtaccc attgagcctc atgctctgtt cttgggaatc       180
catggaggga agatgtgcct gtcctgtgtc aagtctggta atgagaccag actccagctg       240
gaggcagtta acatcactga cctgagcgag aacagaaagc aggacaagcg cttcgccttc       300
atccgctcag acagcggccc caccaccagt tttgagtctg ccgcctgccc cggttggttc       360
ctctgcacag cgatggaagc tgaccagccc gtcagcctca ccaatatgcc tgacgaaggc       420
gtcatggtca ccaaattcta cttccaggag gacgag                                 456

<210>  13
<211>  152
<212>  PRT
<213>  IL-1RN  R14T+D74N Protein Sequence

<400>  13
Arg Pro Ser Gly Arg Lys Ser Ser Lys Met Gln Ala Phe Thr Ile Trp 
1               5                   10                  15      
Asp Val Asn Gln Lys Thr Phe Tyr Leu Arg Asn Asn Gln Leu Val Ala 
            20                  25                  30          
Gly Tyr Leu Gln Gly Pro Asn Val Asn Leu Glu Glu Lys Ile Asp Val 
        35                  40                  45              
Val Pro Ile Glu Pro His Ala Leu Phe Leu Gly Ile His Gly Gly Lys 
    50                  55                  60                  
Met Cys Leu Ser Cys Val Lys Ser Gly Asn Glu Thr Arg Leu Gln Leu 
65                  70                  75                  80  
Glu Ala Val Asn Ile Thr Asp Leu Ser Glu Asn Arg Lys Gln Asp Lys 
                85                  90                  95      
Arg Phe Ala Phe Ile Arg Ser Asp Ser Gly Pro Thr Thr Ser Phe Glu 
            100                 105                 110         
Ser Ala Ala Cys Pro Gly Trp Phe Leu Cys Thr Ala Met Glu Ala Asp 
        115                 120                 125             
Gln Pro Val Ser Leu Thr Asn Met Pro Asp Glu Gly Val Met Val Thr 
    130                 135                 140                 
Lys Phe Tyr Phe Gln Glu Asp Glu 
145                 150         

<210>  14
<211>  456
<212>  DNA
<213>  IL-1RN  R14T+D74N Nucleotide Sequence

<400>  14
cgaccctctg ggagaaaatc cagcaagatg caagccttca caatctggga tgttaaccag        60
aagaccttct atctgaggaa caaccaacta gttgctggat acttgcaagg accaaatgtc       120
aatttagaag aaaagataga tgtggtaccc attgagcctc atgctctgtt cttgggaatc       180
catggaggga agatgtgcct gtcctgtgtc aagtctggta atgagaccag actccagctg       240
gaggcagtta acatcactga cctgagcgag aacagaaagc aggacaagcg cttcgccttc       300
atccgctcag acagcggccc caccaccagt tttgagtctg ccgcctgccc cggttggttc       360
ctctgcacag cgatggaagc tgaccagccc gtcagcctca ccaatatgcc tgacgaaggc       420
gtcatggtca ccaaattcta cttccaggag gacgag                                 456

<210>  15
<211>  152
<212>  PRT
<213>  IL-1RN R5T,R14T,D74N Protein Sequence

<400>  15
Arg Pro Ser Gly Thr Lys Ser Ser Lys Met Gln Ala Phe Thr Ile Trp 
1               5                   10                  15      
Asp Val Asn Gln Lys Thr Phe Tyr Leu Arg Asn Asn Gln Leu Val Ala 
            20                  25                  30          
Gly Tyr Leu Gln Gly Pro Asn Val Asn Leu Glu Glu Lys Ile Asp Val 
        35                  40                  45              
Val Pro Ile Glu Pro His Ala Leu Phe Leu Gly Ile His Gly Gly Lys 
    50                  55                  60                  
Met Cys Leu Ser Cys Val Lys Ser Gly Asn Glu Thr Arg Leu Gln Leu 
65                  70                  75                  80  
Glu Ala Val Asn Ile Thr Asp Leu Ser Glu Asn Arg Lys Gln Asp Lys 
                85                  90                  95      
Arg Phe Ala Phe Ile Arg Ser Asp Ser Gly Pro Thr Thr Ser Phe Glu 
            100                 105                 110         
Ser Ala Ala Cys Pro Gly Trp Phe Leu Cys Thr Ala Met Glu Ala Asp 
        115                 120                 125             
Gln Pro Val Ser Leu Thr Asn Met Pro Asp Glu Gly Val Met Val Thr 
    130                 135                 140                 
Lys Phe Tyr Phe Gln Glu Asp Glu 
145                 150         

<210>  16
<211>  456
<212>  DNA
<213>  IL-1RN R5T,R14T,D74N Nucleotide Sequence

<400>  16
cgaccctctg ggacaaaatc cagcaagatg caagccttca caatctggga tgttaaccag        60
aagaccttct atctgaggaa caaccaacta gttgctggat acttgcaagg accaaatgtc       120
aatttagaag aaaagataga tgtggtaccc attgagcctc atgctctgtt cttgggaatc       180
catggaggga agatgtgcct gtcctgtgtc aagtctggta atgagaccag actccagctg       240
gaggcagtta acatcactga cctgagcgag aacagaaagc aggacaagcg cttcgccttc       300
atccgctcag acagcggccc caccaccagt tttgagtctg ccgcctgccc cggttggttc       360
ctctgcacag cgatggaagc tgaccagccc gtcagcctca ccaatatgcc tgacgaaggc       420
gtcatggtca ccaaattcta cttccaggag gacgag                                 456

<210>  17
<211>  235
<212>  PRT
<213>  TNFR2 Protein Sequence

<400>  17
Leu Pro Ala Gln Val Ala Phe Thr Pro Tyr Ala Pro Glu Pro Gly Ser 
1               5                   10                  15      
Thr Cys Arg Leu Arg Glu Tyr Tyr Asp Gln Thr Ala Gln Met Cys Cys 
            20                  25                  30          
Ser Lys Cys Ser Pro Gly Gln His Ala Lys Val Phe Cys Thr Lys Thr 
        35                  40                  45              
Ser Asp Thr Val Cys Asp Ser Cys Glu Asp Ser Thr Tyr Thr Gln Leu 
    50                  55                  60                  
Trp Asn Trp Val Pro Glu Cys Leu Ser Cys Gly Ser Arg Cys Ser Ser 
65                  70                  75                  80  
Asp Gln Val Glu Thr Gln Ala Cys Thr Arg Glu Gln Asn Arg Ile Cys 
                85                  90                  95      
Thr Cys Arg Pro Gly Trp Tyr Cys Ala Leu Ser Lys Gln Glu Gly Cys 
            100                 105                 110         
Arg Leu Cys Ala Pro Leu Arg Lys Cys Arg Pro Gly Phe Gly Val Ala 
        115                 120                 125             
Arg Pro Gly Thr Glu Thr Ser Asp Val Val Cys Lys Pro Cys Ala Pro 
    130                 135                 140                 
Gly Thr Phe Ser Asn Thr Thr Ser Ser Thr Asp Ile Cys Arg Pro His 
145                 150                 155                 160 
Gln Ile Cys Asn Val Val Ala Ile Pro Gly Asn Ala Ser Met Asp Ala 
                165                 170                 175     
Val Cys Thr Ser Thr Ser Pro Thr Arg Ser Met Ala Pro Gly Ala Val 
            180                 185                 190         
His Leu Pro Gln Pro Val Ser Thr Arg Ser Gln His Thr Gln Pro Thr 
        195                 200                 205             
Pro Glu Pro Ser Thr Ala Pro Ser Thr Ser Phe Leu Leu Pro Met Gly 
    210                 215                 220                 
Pro Ser Pro Pro Ala Glu Gly Ser Thr Gly Asp 
225                 230                 235 

<210>  18
<211>  232
<212>  PRT
<213>  IgG1Fc Protein Sequence

<400>  18
Glu Pro Lys Ser Cys Asp Lys Thr His Thr Cys Pro Pro Cys Pro Ala 
1               5                   10                  15      
Pro Glu Leu Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro 
            20                  25                  30          
Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val 
        35                  40                  45              
Val Asp Val Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr Val 
    50                  55                  60                  
Asp Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln 
65                  70                  75                  80  
Tyr Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln 
                85                  90                  95      
Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala 
            100                 105                 110         
Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro 
        115                 120                 125             
Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Asp Glu Leu Thr 
    130                 135                 140                 
Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser 
145                 150                 155                 160 
Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr 
                165                 170                 175     
Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr 
            180                 185                 190         
Ser Lys Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe 
        195                 200                 205             
Ser Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys 
    210                 215                 220                 
Ser Leu Ser Leu Ser Pro Gly Lys 
225                 230         

<210>  19
<211>  232
<212>  PRT
<213>  IgG1Fc Mutant Protein Sequence

<400>  19
Glu Pro Lys Ser Cys Asp Lys Thr His Thr Cys Pro Pro Cys Pro Ala 
1               5                   10                  15      
Pro Glu Ala Glu Gly Ala Pro Ser Val Phe Leu Phe Pro Pro Lys Pro 
            20                  25                  30          
Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val 
        35                  40                  45              
Val Asp Val Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr Val 
    50                  55                  60                  
Asp Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln 
65                  70                  75                  80  
Tyr Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln 
                85                  90                  95      
Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala 
            100                 105                 110         
Leu Pro Ser Ser Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro 
        115                 120                 125             
Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Asp Glu Leu Thr 
    130                 135                 140                 
Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser 
145                 150                 155                 160 
Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr 
                165                 170                 175     
Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr 
            180                 185                 190         
Ser Lys Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe 
        195                 200                 205             
Ser Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys 
    210                 215                 220                 
Ser Leu Ser Leu Ser Pro Gly Lys 
225                 230         

<210>  20
<211>  228
<212>  PRT
<213>  IgG2Fc Protein Sequence

<400>  20
Glu Arg Lys Cys Cys Val Glu Cys Pro Pro Cys Pro Ala Pro Pro Val 
1               5                   10                  15      
Ala Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu 
            20                  25                  30          
Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser 
        35                  40                  45              
His Glu Asp Pro Glu Val Gln Phe Asn Trp Tyr Val Asp Gly Met Glu 
    50                  55                  60                  
Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Phe Asn Ser Thr 
65                  70                  75                  80  
Phe Arg Val Val Ser Val Leu Thr Val Val His Gln Asp Trp Leu Asn 
                85                  90                  95      
Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Gly Leu Pro Ala Pro 
            100                 105                 110         
Ile Glu Lys Thr Ile Ser Lys Thr Lys Gly Gln Pro Arg Glu Pro Gln 
        115                 120                 125             
Val Tyr Thr Leu Pro Pro Ser Arg Glu Glu Met Thr Lys Asn Gln Val 
    130                 135                 140                 
Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val 
145                 150                 155                 160 
Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro 
                165                 170                 175     
Pro Met Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr 
            180                 185                 190         
Val Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val 
        195                 200                 205             
Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu 
    210                 215                 220                 
Ser Pro Gly Lys 
225             

<210>  21
<211>  229
<212>  PRT
<213>  IgG4Fc Mutant Protein Sequence

<400>  21
Glu Ser Lys Tyr Gly Pro Pro Cys Pro Pro Cys Pro Ala Pro Glu Phe 
1               5                   10                  15      
Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr 
            20                  25                  30          
Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val 
        35                  40                  45              
Ser Gln Glu Asp Pro Glu Val Gln Phe Asn Trp Tyr Val Asp Gly Val 
    50                  55                  60                  
Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Phe Asn Ser 
65                  70                  75                  80  
Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu 
                85                  90                  95      
Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Gly Leu Pro Ser 
            100                 105                 110         
Ser Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro 
        115                 120                 125             
Gln Val Tyr Thr Leu Pro Pro Ser Gln Glu Glu Met Thr Lys Asn Gln 
    130                 135                 140                 
Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala 
145                 150                 155                 160 
Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr 
                165                 170                 175     
Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Arg Leu 
            180                 185                 190         
Thr Val Asp Lys Ser Arg Trp Gln Glu Gly Asn Val Phe Ser Cys Ser 
        195                 200                 205             
Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser 
    210                 215                 220                 
Leu Ser Leu Gly Lys 
225                 

<210>  22
<211>  585
<212>  PRT
<213>  HSA Protein Sequence

<400>  22
Asp Ala His Lys Ser Glu Val Ala His Arg Phe Lys Asp Leu Gly Glu 
1               5                   10                  15      
Glu Asn Phe Lys Ala Leu Val Leu Ile Ala Phe Ala Gln Tyr Leu Gln 
            20                  25                  30          
Gln Cys Pro Phe Glu Asp His Val Lys Leu Val Asn Glu Val Thr Glu 
        35                  40                  45              
Phe Ala Lys Thr Cys Val Ala Asp Glu Ser Ala Glu Asn Cys Asp Lys 
    50                  55                  60                  
Ser Leu His Thr Leu Phe Gly Asp Lys Leu Cys Thr Val Ala Thr Leu 
65                  70                  75                  80  
Arg Glu Thr Tyr Gly Glu Met Ala Asp Cys Cys Ala Lys Gln Glu Pro 
                85                  90                  95      
Glu Arg Asn Glu Cys Phe Leu Gln His Lys Asp Asp Asn Pro Asn Leu 
            100                 105                 110         
Pro Arg Leu Val Arg Pro Glu Val Asp Val Met Cys Thr Ala Phe His 
        115                 120                 125             
Asp Asn Glu Glu Thr Phe Leu Lys Lys Tyr Leu Tyr Glu Ile Ala Arg 
    130                 135                 140                 
Arg His Pro Tyr Phe Tyr Ala Pro Glu Leu Leu Phe Phe Ala Lys Arg 
145                 150                 155                 160 
Tyr Lys Ala Ala Phe Thr Glu Cys Cys Gln Ala Ala Asp Lys Ala Ala 
                165                 170                 175     
Cys Leu Leu Pro Lys Leu Asp Glu Leu Arg Asp Glu Gly Lys Ala Ser 
            180                 185                 190         
Ser Ala Lys Gln Arg Leu Lys Cys Ala Ser Leu Gln Lys Phe Gly Glu 
        195                 200                 205             
Arg Ala Phe Lys Ala Trp Ala Val Ala Arg Leu Ser Gln Arg Phe Pro 
    210                 215                 220                 
Lys Ala Glu Phe Ala Glu Val Ser Lys Leu Val Thr Asp Leu Thr Lys 
225                 230                 235                 240 
Val His Thr Glu Cys Cys His Gly Asp Leu Leu Glu Cys Ala Asp Asp 
                245                 250                 255     
Arg Ala Asp Leu Ala Lys Tyr Ile Cys Glu Asn Gln Asp Ser Ile Ser 
            260                 265                 270         
Ser Lys Leu Lys Glu Cys Cys Glu Lys Pro Leu Leu Glu Lys Ser His 
        275                 280                 285             
Cys Ile Ala Glu Val Glu Asn Asp Glu Met Pro Ala Asp Leu Pro Ser 
    290                 295                 300                 
Leu Ala Ala Asp Phe Val Glu Ser Lys Asp Val Cys Lys Asn Tyr Ala 
305                 310                 315                 320 
Glu Ala Lys Asp Val Phe Leu Gly Met Phe Leu Tyr Glu Tyr Ala Arg 
                325                 330                 335     
Arg His Pro Asp Tyr Ser Val Val Leu Leu Leu Arg Leu Ala Lys Thr 
            340                 345                 350         
Tyr Glu Thr Thr Leu Glu Lys Cys Cys Ala Ala Ala Asp Pro His Glu 
        355                 360                 365             
Cys Tyr Ala Lys Val Phe Asp Glu Phe Lys Pro Leu Val Glu Glu Pro 
    370                 375                 380                 
Gln Asn Leu Ile Lys Gln Asn Cys Glu Leu Phe Glu Gln Leu Gly Glu 
385                 390                 395                 400 
Tyr Lys Phe Gln Asn Ala Leu Leu Val Arg Tyr Thr Lys Lys Val Pro 
                405                 410                 415     
Gln Val Ser Thr Pro Thr Leu Val Glu Val Ser Arg Asn Leu Gly Lys 
            420                 425                 430         
Val Gly Ser Lys Cys Cys Lys His Pro Glu Ala Lys Arg Met Pro Cys 
        435                 440                 445             
Ala Glu Asp Tyr Leu Ser Val Val Leu Asn Gln Leu Cys Val Leu His 
    450                 455                 460                 
Glu Lys Thr Pro Val Ser Asp Arg Val Thr Lys Cys Cys Thr Glu Ser 
465                 470                 475                 480 
Leu Val Asn Arg Arg Pro Cys Phe Ser Ala Leu Glu Val Asp Glu Thr 
                485                 490                 495     
Tyr Val Pro Lys Glu Phe Asn Ala Glu Thr Phe Thr Phe His Ala Asp 
            500                 505                 510         
Ile Cys Thr Leu Ser Glu Lys Glu Arg Gln Ile Lys Lys Gln Thr Ala 
        515                 520                 525             
Leu Val Glu Leu Val Lys His Lys Pro Lys Ala Thr Lys Glu Gln Leu 
    530                 535                 540                 
Lys Ala Val Met Asp Asp Phe Ala Ala Phe Val Glu Lys Cys Cys Lys 
545                 550                 555                 560 
Ala Asp Asp Lys Glu Thr Cys Phe Ala Glu Glu Gly Lys Lys Leu Val 
                565                 570                 575     
Ala Ala Ser Gln Ala Ala Leu Gly Leu 
            580                 585 

<210>  23
<211>  396
<212>  PRT
<213>  IL-1RN D74N-hIgG4 Fc S228P Protein Sequence

<400>  23
Arg Pro Ser Gly Arg Lys Ser Ser Lys Met Gln Ala Phe Arg Ile Trp 
1               5                   10                  15      
Asp Val Asn Gln Lys Thr Phe Tyr Leu Arg Asn Asn Gln Leu Val Ala 
            20                  25                  30          
Gly Tyr Leu Gln Gly Pro Asn Val Asn Leu Glu Glu Lys Ile Asp Val 
        35                  40                  45              
Val Pro Ile Glu Pro His Ala Leu Phe Leu Gly Ile His Gly Gly Lys 
    50                  55                  60                  
Met Cys Leu Ser Cys Val Lys Ser Gly Asn Glu Thr Arg Leu Gln Leu 
65                  70                  75                  80  
Glu Ala Val Asn Ile Thr Asp Leu Ser Glu Asn Arg Lys Gln Asp Lys 
                85                  90                  95      
Arg Phe Ala Phe Ile Arg Ser Asp Ser Gly Pro Thr Thr Ser Phe Glu 
            100                 105                 110         
Ser Ala Ala Cys Pro Gly Trp Phe Leu Cys Thr Ala Met Glu Ala Asp 
        115                 120                 125             
Gln Pro Val Ser Leu Thr Asn Met Pro Asp Glu Gly Val Met Val Thr 
    130                 135                 140                 
Lys Phe Tyr Phe Gln Glu Asp Glu Gly Gly Gly Gly Ser Gly Gly Gly 
145                 150                 155                 160 
Gly Ser Gly Gly Gly Gly Ser Glu Ser Lys Tyr Gly Pro Pro Cys Pro 
                165                 170                 175     
Pro Cys Pro Ala Pro Glu Phe Leu Gly Gly Pro Ser Val Phe Leu Phe 
            180                 185                 190         
Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val 
        195                 200                 205             
Thr Cys Val Val Val Asp Val Ser Gln Glu Asp Pro Glu Val Gln Phe 
    210                 215                 220                 
Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr Lys Pro 
225                 230                 235                 240 
Arg Glu Glu Gln Phe Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr 
                245                 250                 255     
Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val 
            260                 265                 270         
Ser Asn Lys Gly Leu Pro Ser Ser Ile Glu Lys Thr Ile Ser Lys Ala 
        275                 280                 285             
Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Gln 
    290                 295                 300                 
Glu Glu Met Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly 
305                 310                 315                 320 
Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro 
                325                 330                 335     
Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser 
            340                 345                 350         
Phe Phe Leu Tyr Ser Arg Leu Thr Val Asp Lys Ser Arg Trp Gln Glu 
        355                 360                 365             
Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala Leu His Asn His 
    370                 375                 380                 
Tyr Thr Gln Lys Ser Leu Ser Leu Ser Leu Gly Lys 
385                 390                 395     

<210>  24
<211>  396
<212>  PRT
<213>  hIgG4 Fc S228P-IL-1RN D74N Protein Sequence

<400>  24
Glu Ser Lys Tyr Gly Pro Pro Cys Pro Pro Cys Pro Ala Pro Glu Phe 
1               5                   10                  15      
Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr 
            20                  25                  30          
Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val 
        35                  40                  45              
Ser Gln Glu Asp Pro Glu Val Gln Phe Asn Trp Tyr Val Asp Gly Val 
    50                  55                  60                  
Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Phe Asn Ser 
65                  70                  75                  80  
Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu 
                85                  90                  95      
Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Gly Leu Pro Ser 
            100                 105                 110         
Ser Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro 
        115                 120                 125             
Gln Val Tyr Thr Leu Pro Pro Ser Gln Glu Glu Met Thr Lys Asn Gln 
    130                 135                 140                 
Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala 
145                 150                 155                 160 
Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr 
                165                 170                 175     
Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Arg Leu 
            180                 185                 190         
Thr Val Asp Lys Ser Arg Trp Gln Glu Gly Asn Val Phe Ser Cys Ser 
        195                 200                 205             
Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser 
    210                 215                 220                 
Leu Ser Leu Gly Lys Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly 
225                 230                 235                 240 
Gly Gly Gly Ser Arg Pro Ser Gly Arg Lys Ser Ser Lys Met Gln Ala 
                245                 250                 255     
Phe Arg Ile Trp Asp Val Asn Gln Lys Thr Phe Tyr Leu Arg Asn Asn 
            260                 265                 270         
Gln Leu Val Ala Gly Tyr Leu Gln Gly Pro Asn Val Asn Leu Glu Glu 
        275                 280                 285             
Lys Ile Asp Val Val Pro Ile Glu Pro His Ala Leu Phe Leu Gly Ile 
    290                 295                 300                 
His Gly Gly Lys Met Cys Leu Ser Cys Val Lys Ser Gly Asn Glu Thr 
305                 310                 315                 320 
Arg Leu Gln Leu Glu Ala Val Asn Ile Thr Asp Leu Ser Glu Asn Arg 
                325                 330                 335     
Lys Gln Asp Lys Arg Phe Ala Phe Ile Arg Ser Asp Ser Gly Pro Thr 
            340                 345                 350         
Thr Ser Phe Glu Ser Ala Ala Cys Pro Gly Trp Phe Leu Cys Thr Ala 
        355                 360                 365             
Met Glu Ala Asp Gln Pro Val Ser Leu Thr Asn Met Pro Asp Glu Gly 
    370                 375                 380                 
Val Met Val Thr Lys Phe Tyr Phe Gln Glu Asp Glu 
385                 390                 395     

