                               SEQUENCE LISTING

<110> MERCK SHARP & DOHME CORP.
 
<120> REDUCTASE ENZYMES AND PROCESSES FOR MAKING AND USING REDUCTASE
      ENZYMES

<130> 24998

<140>
<141>

<150> 63/028,769
<151> 2020-05-22

<160> 11    

<170> PatentIn version 3.5

<210> 1
<211> 230
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polypeptide

<220>
<223> His-tagged wild-type ketoreductase

<400> 1
Met His His His His His His Asn Lys Thr Ile Val Val Thr Gly Gly 
1               5                   10                  15      


Thr Lys Gly Ile Gly Arg Ala Ile Val Glu Lys Phe Ala Lys Glu Gly 
            20                  25                  30          


Phe Thr Val Leu Thr Cys Ala Arg Thr Lys Gly Asp Asn Phe Pro Glu 
        35                  40                  45              


Asn Val His Phe Phe Lys Ala Asp Leu Ser Lys Lys Val Glu Val Leu 
    50                  55                  60                  


Ala Phe Ala Asp Phe Ile Lys Gln Thr Val Asn Gln Val Asp Ile Leu 
65                  70                  75                  80  


Val Asn Asn Thr Gly Phe Phe Leu Pro Gly Glu Ile Asn Asn Glu Ala 
                85                  90                  95      


Glu Gly Thr Leu Glu Ala Met Ile Glu Thr Asn Leu Tyr Ser Ala Tyr 
            100                 105                 110         


Tyr Leu Thr Arg Ala Leu Val Gly Asp Met Ile Thr Lys Lys Glu Gly 
        115                 120                 125             


His Ile Phe Asn Ile Cys Ser Thr Ala Ser Ile Thr Ala Tyr Thr Asn 
    130                 135                 140                 


Gly Gly Ser Tyr Cys Ile Ser Lys Phe Ala Leu Leu Gly Met Ser Lys 
145                 150                 155                 160 


Val Leu Arg Glu Glu Leu Lys Pro His His Val Arg Val Thr Ser Ile 
                165                 170                 175     


Leu Pro Gly Ala Thr Leu Thr Asp Ser Trp Ala Gly Val Glu Leu Pro 
            180                 185                 190         


Ala Glu Arg Phe Ile Ala Ser Glu Asp Ile Ala Gln Ile Val Trp Thr 
        195                 200                 205             


Ala His Cys Leu Pro Ser Thr Thr Val Leu Glu Glu Ile Leu Ile Arg 
    210                 215                 220                 


Pro Gln Leu Gly Asp Leu 
225                 230 


<210> 2
<211> 230
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polypeptide

<220>
<223> Artificial  ketoreductase

<400> 2
Met His His His His His His Asn Lys Thr Ile Val Val Thr Gly Gly 
1               5                   10                  15      


Thr Lys Gly Ile Gly Arg Ala Ile Val Glu Lys Phe Ala Lys Glu Gly 
            20                  25                  30          


Phe Thr Val Leu Thr Cys Ala Arg Thr Lys Gly Asp Asn Phe Pro Glu 
        35                  40                  45              


Asn Val His Phe Phe Lys Ala Asp Leu Ser Lys Lys Val Glu Val Leu 
    50                  55                  60                  


Ala Phe Ala Asp Phe Ile Lys Gln Thr Val Asn Gln Val Asp Ile Leu 
65                  70                  75                  80  


Val Asn Asn Thr Gly Phe Phe Leu Pro Gly Glu Ile Asn Asn Glu Ala 
                85                  90                  95      


Glu Gly Thr Leu Glu Ala Met Ile Glu Thr Asn Leu Tyr Ser Ala Tyr 
            100                 105                 110         


Tyr Leu Thr Arg Ala Leu Val Gly Asp Met Ile Thr Lys Lys Glu Gly 
        115                 120                 125             


His Ile Phe Asn Ile Cys Ser Thr Ala Ser Ile Thr Ala Tyr Thr Asn 
    130                 135                 140                 


Gly Gly Ser Tyr Cys Ile Ser Lys Phe Ala Leu Leu Gly Met Ser Lys 
145                 150                 155                 160 


Val Leu Arg Glu Glu Leu Lys Pro His His Val Arg Val Thr Ser Ile 
                165                 170                 175     


Leu Pro Gly Ala Thr Leu Asn Asp Ser Trp Ala Gly Val Glu Leu Pro 
            180                 185                 190         


Ala Glu Arg Phe Ile Ala Ser Glu Asp Ile Ala Gln Ile Val Trp Thr 
        195                 200                 205             


Ala His Cys Leu Pro Ser Thr Thr Val Leu Glu Glu Ile Leu Ile Arg 
    210                 215                 220                 


Pro Gln Leu Gly Asp Leu 
225                 230 


<210> 3
<211> 230
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polypeptide

<220>
<223> Artificial ketoreductase

<400> 3
Met His His His His His His Asn Lys Thr Ile Val Val Thr Gly Gly 
1               5                   10                  15      


Thr Lys Gly Ile Gly Arg Ala Ile Val Glu Lys Phe Ala Lys Glu Gly 
            20                  25                  30          


Phe Thr Val Leu Thr Cys Ala Arg Thr Lys Gly Asp Asn Phe Pro Glu 
        35                  40                  45              


Asn Val His Phe Phe Lys Ala Asp Leu Ser Lys Lys Val Glu Val Leu 
    50                  55                  60                  


Ala Phe Ala Asp Phe Ile Lys Gln Thr Val Asn Gln Val Asp Ile Leu 
65                  70                  75                  80  


Val Asn Asn Thr Gly Phe Phe Leu Pro Gly Glu Ile Asn Asn Glu Ala 
                85                  90                  95      


Glu Gly Thr Leu Glu Ala Met Ile Glu Thr Asn Leu Tyr Ser Ala Tyr 
            100                 105                 110         


Tyr Leu Thr Arg Ala Leu Val Gly Asp Met Ile Thr Lys Lys Glu Gly 
        115                 120                 125             


His Ile Phe Asn Ile Cys Ser Tyr Ala Ser Ile Thr Ala Tyr Thr Ser 
    130                 135                 140                 


Gly Gly Ser Tyr Cys Ile Ser Lys Phe Ala Leu Leu Gly Met Ser Lys 
145                 150                 155                 160 


Val Leu Arg Glu Glu Leu Lys Pro His His Val Arg Val Thr Ser Ile 
                165                 170                 175     


Leu Pro Gly Ala Thr Leu Asn Asp Ser Trp Ala Lys Val Glu Leu Pro 
            180                 185                 190         


Ala Glu Arg Phe Ile Ala Ser Glu Asp Ile Ala Gln Ile Val Trp Thr 
        195                 200                 205             


Ala His Cys Leu Pro Ser Thr Thr Val Leu Glu Glu Ile Leu Ile Arg 
    210                 215                 220                 


Pro Gln Leu Gly Asp Leu 
225                 230 


<210> 4
<211> 229
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polypeptide

<220>
<223> Artificial ketoreductase

<400> 4
Met His His His His His Pro Ala Thr Ile Val Val Thr Gly Gly Thr 
1               5                   10                  15      


Lys Gly Ile Gly Arg Ala Ile Val Glu Lys Phe Ala Lys Glu Gly Phe 
            20                  25                  30          


Thr Val Leu Thr Cys Ala Arg Thr Lys Gly Asp Asn Phe Pro Glu Asn 
        35                  40                  45              


Val His Phe Phe Lys Ala Asp Leu Ser Lys Lys Val Glu Val Leu Ala 
    50                  55                  60                  


Phe Ala Asp Phe Ile Lys Gln Thr Val Asn Gln Val Asp Ile Leu Val 
65                  70                  75                  80  


Asn Asn Thr Gly Phe Phe Leu Pro Gly Glu Ile Asn Asn Glu Ala Glu 
                85                  90                  95      


Gly Thr Leu Glu Ala Met Ile Glu Thr Asn Leu Tyr Ser Ala Tyr Tyr 
            100                 105                 110         


Leu Thr Arg Ala Leu Val Gly Asp Met Ile Thr Lys Lys Glu Gly His 
        115                 120                 125             


Ile Phe Asn Ile Cys Ser Tyr Ala Ser Ile Thr Ala Tyr Thr Ser Gly 
    130                 135                 140                 


Gly Ser Tyr Cys Ile Ser Lys Thr Ala Leu Leu Gly Met Ser Lys Val 
145                 150                 155                 160 


Leu Arg Glu Glu Leu Lys Pro His His Val Arg Val Thr Ser Ile Leu 
                165                 170                 175     


Pro Gly Ala Thr Leu Asn Asp Ser Trp Ala Lys Val Glu Leu Pro Ala 
            180                 185                 190         


Glu Trp Phe Ile Ala Ser Glu Asp Ile Ala Gln Ile Val Trp Thr Ala 
        195                 200                 205             


His Cys Leu Pro Ser Thr Thr Val Leu Glu Glu Ile Leu Ile Arg Pro 
    210                 215                 220                 


Gln Leu Gly Asp Leu 
225                 


<210> 5
<211> 229
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polypeptide

<220>
<223> Artificial ketoreductase

<400> 5
Met His His His His His Pro Ala Thr Ile Val Val Thr Gly Gly Thr 
1               5                   10                  15      


Lys Gly Ile Gly Arg Ala Ile Val Glu Lys Phe Ala Lys Glu Gly Phe 
            20                  25                  30          


Thr Val Leu Thr Cys Ala Arg Thr Lys Gly Asp Asn Phe Pro Glu Asn 
        35                  40                  45              


Val His Phe Phe Lys Ala Asp Leu Ser Lys Lys Val Glu Val Leu Ala 
    50                  55                  60                  


Phe Ala Asp Phe Ile Lys Gln Thr Val Asn Gln Val Asp Ile Leu Val 
65                  70                  75                  80  


Asn Asn Thr Gly Trp Phe Leu Pro Gly Glu Ile Asn Asn Glu Ala Glu 
                85                  90                  95      


Gly Thr Leu Glu Ala Met Ile Glu Thr Asn Leu Tyr Ser Ala Tyr Tyr 
            100                 105                 110         


Leu Thr Arg Ala Leu Val Gly Asp Met Ile Thr Lys Lys Glu Gly His 
        115                 120                 125             


Ile Phe Asn Ile Cys Ser Tyr Ala Ser Ile Val Pro Tyr Thr Ser Gly 
    130                 135                 140                 


Gly Ser Tyr Cys Ile Ser Lys Thr Ala Gln Leu Gly Met Ser Lys Val 
145                 150                 155                 160 


Leu Arg Glu Glu Leu Lys Pro His His Val Arg Val Thr Ser Ile Leu 
                165                 170                 175     


Pro Gly Ala Val Leu Asn Asp Ser Trp Ala Lys Val Glu Leu Pro Ala 
            180                 185                 190         


Glu Trp Phe Ile Ala Ser Glu Asp Ile Ala Gln Ile Val Trp Thr Ala 
        195                 200                 205             


His Cys Leu Pro Ser Thr Thr Val Leu Glu Glu Ile Leu Ile Arg Pro 
    210                 215                 220                 


Gln Leu Gly Asp Leu 
225                 


<210> 6
<211> 229
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polypeptide

<220>
<223> Artificial ketoreductase

<400> 6
Met His His His His His Pro Ala Thr Ile Val Val Thr Gly Gly Thr 
1               5                   10                  15      


Lys Gly Ile Gly Arg Ala Ile Val Glu Lys Phe Ala Lys Glu Gly Phe 
            20                  25                  30          


Thr Val Leu Thr Cys Ala Arg Thr Lys Gly Asp Asn Phe Pro Glu Asn 
        35                  40                  45              


Val His Phe Phe Lys Ala Asp Leu Ser Lys Lys Val Glu Val Leu Ala 
    50                  55                  60                  


Phe Ala Asp Phe Ile Lys Gln Thr Val Asn Gln Val Asp Ile Leu Val 
65                  70                  75                  80  


Asn Asn Thr Gly Trp Phe Leu Pro Gly Glu Ile Asn Asn Glu Ala Glu 
                85                  90                  95      


Gly Thr Leu Glu Ala Met Ile Glu Thr Asn Leu Tyr Ser Ala Tyr Tyr 
            100                 105                 110         


Leu Thr Arg Ala Leu Val Gly Asp Met Ile Thr Lys Lys Glu Gly His 
        115                 120                 125             


Ile Phe Asn Ile Cys Ser Tyr Ala Ser Ile Val Pro Tyr Thr Ser Gly 
    130                 135                 140                 


Gly Ser Tyr Cys Ile Ser Lys Thr Ala Gln Leu Gly Met Ser Lys Val 
145                 150                 155                 160 


Leu Arg Glu Glu Leu Lys Pro His His Val Arg Val Thr Ser Ile Leu 
                165                 170                 175     


Pro Gly Ala Val Leu Asn Asp Ser Trp Ala Lys Val Glu Leu Pro Ala 
            180                 185                 190         


Glu Leu Phe Ile Ala Pro Glu Asp Ile Ala Gln Ile Val Trp Thr Ala 
        195                 200                 205             


His Cys Leu Pro Ser Thr Thr Val Leu Glu Glu Ile Leu Ile Arg Pro 
    210                 215                 220                 


Gln Thr Gly Asp Leu 
225                 


<210> 7
<211> 229
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polypeptide

<220>
<223> Artificial ketoreductase

<400> 7
Met His His His His His Pro Ala Thr Ile Val Val Thr Gly Gly Thr 
1               5                   10                  15      


Lys Gly Ile Gly Arg Ala Ile Val Glu Lys Phe Ala Lys Glu Gly Phe 
            20                  25                  30          


Thr Val Leu Thr Cys Ala Arg Thr Ala Gly Asp Asn Phe Pro Glu Asn 
        35                  40                  45              


Val His Phe Phe Lys Ala Asp Leu Ser Lys Lys Val Glu Val Leu Ala 
    50                  55                  60                  


Phe Ala Asp Phe Ile Lys Gln Thr Val Asn Gln Val Asp Ile Leu Val 
65                  70                  75                  80  


Asn Asn Thr Gly Trp Phe Leu Pro Gly Glu Ile Asn Asn Glu Glu Glu 
                85                  90                  95      


Gly Thr Leu Glu Ala Met Ile Glu Thr Asn Leu Tyr Ser Ala Tyr Tyr 
            100                 105                 110         


Leu Thr Arg Ala Leu Val Gly Asp Met Ile Thr Lys Lys Glu Gly His 
        115                 120                 125             


Ile Phe Asn Ile Cys Ser Tyr Ala Ser Ile Val Pro Tyr Thr Ser Gly 
    130                 135                 140                 


Gly Ser Tyr Cys Ile Ser Lys Thr Ala Gln Leu Gly Met Ser Lys Val 
145                 150                 155                 160 


Leu Arg Glu Glu Leu Lys Pro His His Val Arg Val Thr Ser Ile Leu 
                165                 170                 175     


Pro Gly Ala Val Leu Asn Asp Ser Trp Ala Lys Val Glu Leu Pro Ala 
            180                 185                 190         


Glu Leu Phe Ile Ala Pro Glu Asp Ile Ala Gln Ile Val Trp Thr Ala 
        195                 200                 205             


His Cys Leu Pro Ser Thr Thr Val Leu Glu Glu Ile Leu Ile Arg Pro 
    210                 215                 220                 


Gln Glu Gly Asp Leu 
225                 


<210> 8
<211> 229
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polypeptide

<220>
<223> Artificial ketoreductase

<400> 8
Met His His His His His Pro Ala Thr Ile Val Val Thr Gly Gly Thr 
1               5                   10                  15      


Lys Gly Ile Gly Arg Ala Ile Val Glu Lys Phe Ala Lys Glu Gly Phe 
            20                  25                  30          


Thr Val Leu Thr Cys Ala Arg Thr Ala Gly Asp Asn Phe Pro Glu Asn 
        35                  40                  45              


Val His Phe Phe Lys Ala Asp Leu Ser Lys Lys Val Glu Val Leu Ala 
    50                  55                  60                  


Phe Ala Asp Phe Ile Lys Gln Thr Val Asn Gln Val Asp Ile Leu Val 
65                  70                  75                  80  


Asn Asn Thr Gly His Phe Leu Pro Gly Glu Ile Asn Asn Glu Glu Glu 
                85                  90                  95      


Gly Thr Leu Glu Ala Met Ile Glu Thr Asn Leu Tyr Ser Ala Tyr Tyr 
            100                 105                 110         


Leu Thr Arg Ala Leu Val Gly Asp Met Ile Thr Lys Lys Glu Gly His 
        115                 120                 125             


Ile Phe Asn Ile Cys Ser Tyr Ala Ser Ile Val Pro Tyr Thr Ser Gly 
    130                 135                 140                 


Gly Ser Tyr Cys Ile Ser Lys Thr Ala Glu Leu Gly Met Ser Lys Val 
145                 150                 155                 160 


Leu Arg Glu Glu Leu Lys Pro His His Val Arg Val Thr Ser Ile Leu 
                165                 170                 175     


Pro Gly Ala Val Leu Asn Asp Ser Trp Ala Lys Ala Glu Leu Pro Ala 
            180                 185                 190         


Glu Leu Phe Ile Ala Pro Glu Asp Ile Ala Gln Ile Val Trp Thr Ala 
        195                 200                 205             


His Cys Leu Pro Ser Thr Thr Val Leu Glu Glu Ile Leu Ile Arg Pro 
    210                 215                 220                 


Gln Glu Gly Asp Leu 
225                 


<210> 9
<211> 229
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polypeptide

<220>
<223> Artificial ketoreductase

<400> 9
Met His His His His His Pro Ala Thr Ile Val Val Thr Gly Gly Thr 
1               5                   10                  15      


Lys Gly Ile Gly Arg Ala Ile Val Glu Lys Phe Ala Lys Glu Gly Phe 
            20                  25                  30          


Thr Val Leu Thr Cys Ala Arg Thr Ala Gly Asp Asn Phe Pro Glu Asn 
        35                  40                  45              


Val His Phe Phe Lys Ala Asp Leu Ser Lys Lys Val Glu Val Leu Ala 
    50                  55                  60                  


Phe Ala Asp Phe Ile Lys Gln Thr Val Asn Gln Val Asp Ile Leu Val 
65                  70                  75                  80  


Asn Asn Thr Gly His Phe Leu Pro Gly Glu Ile Asn Asn Glu Glu Glu 
                85                  90                  95      


Gly Thr Leu Glu Ala Met Ile Glu Thr Asn Leu Tyr Ser Ala Tyr Tyr 
            100                 105                 110         


Leu Thr Arg Ala Leu Val Gly Asp Met Ile Thr Lys Lys Glu Gly His 
        115                 120                 125             


Ile Phe Asn Ile Cys Ser Tyr Ala Ser Ile Val Pro Tyr Thr Ser Gly 
    130                 135                 140                 


Gly Ser Tyr Cys Ile Ser Lys Thr Ala Glu Leu Ala Met Ser Arg Val 
145                 150                 155                 160 


Leu Arg Glu Glu Leu Lys Pro His His Val Arg Val Thr Ser Ile Leu 
                165                 170                 175     


Pro Gly Ala Val Leu Asn Asp Asn Trp Ala Lys Ala Glu Leu Pro Ala 
            180                 185                 190         


Glu Leu Phe Ile Ala Pro Glu Asp Ile Ala Gln Ile Val Trp Thr Ala 
        195                 200                 205             


His Cys Leu Pro Ser Thr Thr Val Leu Glu Glu Ile Leu Ile Arg Pro 
    210                 215                 220                 


Thr Glu Gly Asp Leu 
225                 


<210> 10
<211> 229
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polypeptide

<220>
<223> Artificial ketoreductase

<400> 10
Met His His His His His Pro Ala Thr Ile Val Val Thr Gly Gly Thr 
1               5                   10                  15      


Lys Gly Ile Gly Arg Ala Ile Val Glu Lys Phe Ala Lys Glu Gly Phe 
            20                  25                  30          


Thr Val Leu Thr Cys Ala Arg Thr Ala Gly Asp Asn Phe Pro Glu Asn 
        35                  40                  45              


Val His Phe Phe Lys Ala Asp Leu Ser Lys Lys Val Glu Val Leu Ala 
    50                  55                  60                  


Phe Ala Asp Phe Ile Lys Ala Thr Val Asn Gln Val Asp Ile Leu Val 
65                  70                  75                  80  


Asn Asn Thr Gly His Phe Leu Pro Gly Glu Ile Asn Asn Glu Glu Glu 
                85                  90                  95      


Gly Thr Leu Glu Ala Met Ile Glu Thr Asn Leu Tyr Ser Ala Tyr Tyr 
            100                 105                 110         


Leu Thr Arg Ala Leu Val Gly Asp Met Ile Thr Lys Lys Glu Gly His 
        115                 120                 125             


Ile Phe Asn Ile Cys Ser Tyr Ala Ser Ile Val Pro Tyr Thr Pro Gly 
    130                 135                 140                 


Gly Ser Tyr Cys Ile Ser Lys Thr Ala Glu Leu Ala Met Ser Arg Val 
145                 150                 155                 160 


Leu Arg Glu Glu Leu Lys Pro His His Val Arg Val Thr Ser Ile Leu 
                165                 170                 175     


Pro Gly Ala Val Leu Asn Asp Asn Trp Ala Lys Ala Glu Leu Pro Ala 
            180                 185                 190         


Glu Leu Phe Ile Ala Pro Glu Asp Ile Ala Gln Ile Val Trp Thr Ala 
        195                 200                 205             


His Cys Leu Pro Ser Thr Thr Val Leu Glu Glu Ile Leu Ile Arg Pro 
    210                 215                 220                 


Thr Glu Gly Asp Leu 
225                 


<210> 11
<211> 229
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polypeptide

<220>
<223> Artificial ketoreductase

<400> 11
Met His His His His His Pro Ala Thr Ile Val Val Thr Gly Gly Thr 
1               5                   10                  15      


Lys Gly Ile Gly Arg Ala Ile Val Glu Lys Phe Ala Lys Glu Gly Phe 
            20                  25                  30          


Thr Val Leu Thr Cys Ala Arg Thr Ala Gly Asp Asn Phe Pro Glu Asn 
        35                  40                  45              


Val His Phe Phe Lys Ala Asp Leu Ser Lys Lys Val Glu Val Leu Ala 
    50                  55                  60                  


Phe Ala Asp Phe Ile Lys Ala Thr Val Asn Gln Val Asp Ile Leu Val 
65                  70                  75                  80  


Asn Asn Thr Gly His Phe Leu Pro Gly Glu Ile Asn Asn Glu Glu Glu 
                85                  90                  95      


Gly Thr Leu Glu Ala Met Ile Glu Thr Asn Leu Tyr Ser Ala Tyr Tyr 
            100                 105                 110         


Leu Thr Arg Ala Leu Val Gly Asp Met Ile Thr Lys Lys Glu Gly His 
        115                 120                 125             


Ile Phe Asn Ile Cys Ser Tyr Ala Ser Ile Val Pro Tyr Thr Ser Gly 
    130                 135                 140                 


Gly Ser Tyr Cys Ile Ser Lys Thr Ala Met Leu Ala Met Ser Arg Val 
145                 150                 155                 160 


Leu Arg Glu Glu Leu Lys Pro His His Val Arg Val Thr Ser Ile Leu 
                165                 170                 175     


Pro Gly Ala Val Leu Asn Asp Asn Trp Ala Lys Ala Glu Leu Pro Ala 
            180                 185                 190         


Glu Leu Phe Ile Ala Pro Glu Asp Ile Ala Gln Ile Val Trp Thr Ala 
        195                 200                 205             


His Cys Leu Pro Ser Thr Thr Val Leu Glu Glu Ile Leu Ile Arg Pro 
    210                 215                 220                 


Thr Glu Gly Asp Leu 
225                 


