                         SEQUENCE LISTING

<110>  Merus N.V.
 
<120>  Methods and means for the production of IG-like molecules

<130>  P273930EP

<160>  17    

<170>  PatentIn version 3.5


<210>  1
<211>  696
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  MV1708_DE_S364V

<400>  1
tccggaactc ctggggggac cgtcagtctt cctcttcccc ccaaaaccca aggacaccct       60

catgatctcc cggacccctg aggtcacatg cgtggtggtg gacgtgagcc acgaagaccc      120

tgaggtcaag ttcaactggt acgtggacgg cgtggaggtg cataatgcca agacaaagcc      180

gcgggaggag cagtacaaca gcacgtaccg tgtggtcagc gtcctcaccg tcctgcacca      240

ggactggctg aatggcaagg agtacaagtg caaggtttcg aacaaagccc tcccagcccc      300

catcgagaaa accatctcca aagccaaagg gcagccccga gaaccacagg tgtacaccga      360

ccccccatcc cgggaggaga tgaccaagaa ccaggtcgtc ctgacctgcg aggtcaaagg      420

cttctatccc agcgacatcg ccgtggagtg ggagagcaat gggcagccgg agaacaacta      480

caagaccacg cctcccgtgc tggactccga cggctccttc ttcctctata gcaagctcac      540

cgtggacaag agcaggtggc agcaggggaa cgtcttctca tgctccgtga tgcatgaggc      600

tctgcacaac cactacacgc agaagagcct ctccctgtct ccgggtaaat gagtttaacg      660

gatcttaatt aatccgagct cggtaccaag cttaag                                696


<210>  2
<211>  696
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  MV1708_DE_S364L

<400>  2
tccggaactc ctggggggac cgtcagtctt cctcttcccc ccaaaaccca aggacaccct       60

catgatctcc cggacccctg aggtcacatg cgtggtggtg gacgtgagcc acgaagaccc      120

tgaggtcaag ttcaactggt acgtggacgg cgtggaggtg cataatgcca agacaaagcc      180

gcgggaggag cagtacaaca gcacgtaccg tgtggtcagc gtcctcaccg tcctgcacca      240

ggactggctg aatggcaagg agtacaagtg caaggtttcg aacaaagccc tcccagcccc      300

catcgagaaa accatctcca aagccaaagg gcagccccga gaaccacagg tgtacaccga      360

ccccccatcc cgggaggaga tgaccaagaa ccaggtcctc ctgacctgcg aggtcaaagg      420

cttctatccc agcgacatcg ccgtggagtg ggagagcaat gggcagccgg agaacaacta      480

caagaccacg cctcccgtgc tggactccga cggctccttc ttcctctata gcaagctcac      540

cgtggacaag agcaggtggc agcaggggaa cgtcttctca tgctccgtga tgcatgaggc      600

tctgcacaac cactacacgc agaagagcct ctccctgtct ccgggtaaat gagtttaacg      660

gatcttaatt aatccgagct cggtaccaag cttaag                                696


<210>  3
<211>  696
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  MV1708_DE_S364I

<400>  3
tccggaactc ctggggggac cgtcagtctt cctcttcccc ccaaaaccca aggacaccct       60

catgatctcc cggacccctg aggtcacatg cgtggtggtg gacgtgagcc acgaagaccc      120

tgaggtcaag ttcaactggt acgtggacgg cgtggaggtg cataatgcca agacaaagcc      180

gcgggaggag cagtacaaca gcacgtaccg tgtggtcagc gtcctcaccg tcctgcacca      240

ggactggctg aatggcaagg agtacaagtg caaggtttcg aacaaagccc tcccagcccc      300

catcgagaaa accatctcca aagccaaagg gcagccccga gaaccacagg tgtacaccga      360

ccccccatcc cgggaggaga tgaccaagaa ccaggtcatc ctgacctgcg aggtcaaagg      420

cttctatccc agcgacatcg ccgtggagtg ggagagcaat gggcagccgg agaacaacta      480

caagaccacg cctcccgtgc tggactccga cggctccttc ttcctctata gcaagctcac      540

cgtggacaag agcaggtggc agcaggggaa cgtcttctca tgctccgtga tgcatgaggc      600

tctgcacaac cactacacgc agaagagcct ctccctgtct ccgggtaaat gagtttaacg      660

gatcttaatt aatccgagct cggtaccaag cttaag                                696


<210>  4
<211>  696
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  MV1708_DE_S364T

<400>  4
tccggaactc ctggggggac cgtcagtctt cctcttcccc ccaaaaccca aggacaccct       60

catgatctcc cggacccctg aggtcacatg cgtggtggtg gacgtgagcc acgaagaccc      120

tgaggtcaag ttcaactggt acgtggacgg cgtggaggtg cataatgcca agacaaagcc      180

gcgggaggag cagtacaaca gcacgtaccg tgtggtcagc gtcctcaccg tcctgcacca      240

ggactggctg aatggcaagg agtacaagtg caaggtttcg aacaaagccc tcccagcccc      300

catcgagaaa accatctcca aagccaaagg gcagccccga gaaccacagg tgtacaccga      360

ccccccatcc cgggaggaga tgaccaagaa ccaggtcaca ctgacctgcg aggtcaaagg      420

cttctatccc agcgacatcg ccgtggagtg ggagagcaat gggcagccgg agaacaacta      480

caagaccacg cctcccgtgc tggactccga cggctccttc ttcctctata gcaagctcac      540

cgtggacaag agcaggtggc agcaggggaa cgtcttctca tgctccgtga tgcatgaggc      600

tctgcacaac cactacacgc agaagagcct ctccctgtct ccgggtaaat gagtttaacg      660

gatcttaatt aatccgagct cggtaccaag cttaag                                696



<210>  5
<211>  696
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  MV1708_DE_S364Q

<400>  5
tccggaactc ctggggggac cgtcagtctt cctcttcccc ccaaaaccca aggacaccct       60

catgatctcc cggacccctg aggtcacatg cgtggtggtg gacgtgagcc acgaagaccc      120

tgaggtcaag ttcaactggt acgtggacgg cgtggaggtg cataatgcca agacaaagcc      180

gcgggaggag cagtacaaca gcacgtaccg tgtggtcagc gtcctcaccg tcctgcacca      240

ggactggctg aatggcaagg agtacaagtg caaggtttcg aacaaagccc tcccagcccc      300

catcgagaaa accatctcca aagccaaagg gcagccccga gaaccacagg tgtacaccga      360

ccccccatcc cgggaggaga tgaccaagaa ccaggtccag ctgacctgcg aggtcaaagg      420

cttctatccc agcgacatcg ccgtggagtg ggagagcaat gggcagccgg agaacaacta      480

caagaccacg cctcccgtgc tggactccga cggctccttc ttcctctata gcaagctcac      540

cgtggacaag agcaggtggc agcaggggaa cgtcttctca tgctccgtga tgcatgaggc      600

tctgcacaac cactacacgc agaagagcct ctccctgtct ccgggtaaat gagtttaacg      660

gatcttaatt aatccgagct cggtaccaag cttaag                                696


<210>  6
<211>  696
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  MV1708_DE_K409I

<400>  6
tccggaactc ctggggggac cgtcagtctt cctcttcccc ccaaaaccca aggacaccct       60

catgatctcc cggacccctg aggtcacatg cgtggtggtg gacgtgagcc acgaagaccc      120

tgaggtcaag ttcaactggt acgtggacgg cgtggaggtg cataatgcca agacaaagcc      180

gcgggaggag cagtacaaca gcacgtaccg tgtggtcagc gtcctcaccg tcctgcacca      240

ggactggctg aatggcaagg agtacaagtg caaggtttcg aacaaagccc tcccagcccc      300

catcgagaaa accatctcca aagccaaagg gcagccccga gaaccacagg tgtacaccga      360

ccccccatcc cgggaggaga tgaccaagaa ccaggtcagc ctgacctgcg aggtcaaagg      420

cttctatccc agcgacatcg ccgtggagtg ggagagcaat gggcagccgg agaacaacta      480

caagaccacg cctcccgtgc tggactccga cggctccttc ttcctctata gcatcctcac      540

cgtggacaag agcaggtggc agcaggggaa cgtcttctca tgctccgtga tgcatgaggc      600

tctgcacaac cactacacgc agaagagcct ctccctgtct ccgggtaaat gagtttaacg      660

gatcttaatt aatccgagct cggtaccaag cttaag                                696


<210>  7
<211>  696
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  MV1708_DE_K409L

<400>  7
tccggaactc ctggggggac cgtcagtctt cctcttcccc ccaaaaccca aggacaccct       60

catgatctcc cggacccctg aggtcacatg cgtggtggtg gacgtgagcc acgaagaccc      120

tgaggtcaag ttcaactggt acgtggacgg cgtggaggtg cataatgcca agacaaagcc      180

gcgggaggag cagtacaaca gcacgtaccg tgtggtcagc gtcctcaccg tcctgcacca      240

ggactggctg aatggcaagg agtacaagtg caaggtttcg aacaaagccc tcccagcccc      300

catcgagaaa accatctcca aagccaaagg gcagccccga gaaccacagg tgtacaccga      360

ccccccatcc cgggaggaga tgaccaagaa ccaggtcagc ctgacctgcg aggtcaaagg      420

cttctatccc agcgacatcg ccgtggagtg ggagagcaat gggcagccgg agaacaacta      480

caagaccacg cctcccgtgc tggactccga cggctccttc ttcctctata gcctgctcac      540

cgtggacaag agcaggtggc agcaggggaa cgtcttctca tgctccgtga tgcatgaggc      600

tctgcacaac cactacacgc agaagagcct ctccctgtct ccgggtaaat gagtttaacg      660

gatcttaatt aatccgagct cggtaccaag cttaag                                696


<210>  8
<211>  696
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  MV1708_DE_K409E

<400>  8
tccggaactc ctggggggac cgtcagtctt cctcttcccc ccaaaaccca aggacaccct       60

catgatctcc cggacccctg aggtcacatg cgtggtggtg gacgtgagcc acgaagaccc      120

tgaggtcaag ttcaactggt acgtggacgg cgtggaggtg cataatgcca agacaaagcc      180

gcgggaggag cagtacaaca gcacgtaccg tgtggtcagc gtcctcaccg tcctgcacca      240

ggactggctg aatggcaagg agtacaagtg caaggtttcg aacaaagccc tcccagcccc      300

catcgagaaa accatctcca aagccaaagg gcagccccga gaaccacagg tgtacaccga      360

ccccccatcc cgggaggaga tgaccaagaa ccaggtcagc ctgacctgcg aggtcaaagg      420

cttctatccc agcgacatcg ccgtggagtg ggagagcaat gggcagccgg agaacaacta      480

caagaccacg cctcccgtgc tggactccga cggctccttc ttcctctata gcgaactcac      540

cgtggacaag agcaggtggc agcaggggaa cgtcttctca tgctccgtga tgcatgaggc      600

tctgcacaac cactacacgc agaagagcct ctccctgtct ccgggtaaat gagtttaacg      660

gatcttaatt aatccgagct cggtaccaag cttaag                                696



<210>  9
<211>  696
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CH2-3_DE_K360D-S364I

<400>  9
tccggaactc ctggggggac cgtcagtctt cctcttcccc ccaaaaccca aggacaccct       60

catgatctcc cggacccctg aggtcacatg cgtggtggtg gacgtgagcc acgaagaccc      120

tgaggtcaag ttcaactggt acgtggacgg cgtggaggtg cataatgcca agacaaagcc      180

gcgggaggag cagtacaaca gcacgtaccg tgtggtcagc gtcctcaccg tcctgcacca      240

ggactggctg aatggcaagg agtacaagtg caaggtttcg aacaaagccc tcccagcccc      300

catcgagaaa accatctcca aagccaaagg gcagccccga gaaccacagg tgtacaccga      360

ccccccatcc cgggaggaga tgaccgacaa ccaggtcatc ctgacctgcg aggtcaaagg      420

cttctatccc agcgacatcg ccgtggagtg ggagagcaat gggcagccgg agaacaacta      480

caagaccacg cctcccgtgc tggactccga cggctccttc ttcctctata gcaagctcac      540

cgtggacaag agcaggtggc agcaggggaa cgtcttctca tgctccgtga tgcatgaggc      600

tctgcacaac cactacacgc agaagagcct ctccctgtct ccgggtaaat gagtttaacg      660

gatcttaatt aatccgagct cggtaccaag cttaag                                696


<210>  10
<211>  696
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CH2-3_DE_K360D-S364T

<400>  10
tccggaactc ctggggggac cgtcagtctt cctcttcccc ccaaaaccca aggacaccct       60

catgatctcc cggacccctg aggtcacatg cgtggtggtg gacgtgagcc acgaagaccc      120

tgaggtcaag ttcaactggt acgtggacgg cgtggaggtg cataatgcca agacaaagcc      180

gcgggaggag cagtacaaca gcacgtaccg tgtggtcagc gtcctcaccg tcctgcacca      240

ggactggctg aatggcaagg agtacaagtg caaggtttcg aacaaagccc tcccagcccc      300

catcgagaaa accatctcca aagccaaagg gcagccccga gaaccacagg tgtacaccga      360

ccccccatcc cgggaggaga tgaccaagaa ccaggtcctc ctgacctgcg aggtcaaagg      420

cttctatccc agcgacatcg ccgtggagtg ggagagcaat gggcagccgg agaacaacta      480

caagaccacg cctcccgtgc tggactccga cggctccttc ttcctctata gcctgctcac      540

cgtggacaag agcaggtggc agcaggggaa cgtcttctca tgctccgtga tgcatgaggc      600

tctgcacaac cactacacgc agaagagcct ctccctgtct ccgggtaaat gagtttaacg      660

gatcttaatt aatccgagct cggtaccaag cttaag                                696


<210>  11
<211>  696
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CH2-3_DE_K360D-S364V

<400>  11
tccggaactc ctggggggac cgtcagtctt cctcttcccc ccaaaaccca aggacaccct       60

catgatctcc cggacccctg aggtcacatg cgtggtggtg gacgtgagcc acgaagaccc      120

tgaggtcaag ttcaactggt acgtggacgg cgtggaggtg cataatgcca agacaaagcc      180

gcgggaggag cagtacaaca gcacgtaccg tgtggtcagc gtcctcaccg tcctgcacca      240

ggactggctg aatggcaagg agtacaagtg caaggtttcg aacaaagccc tcccagcccc      300

catcgagaaa accatctcca aagccaaagg gcagccccga gaaccacagg tgtacaccga      360

ccccccatcc cgggaggaga tgaccaagaa ccaggtcctc ctgacctgcg aggtcaaagg      420

cttctatccc agcgacatcg ccgtggagtg ggagagcaat gggcagccgg agaacaacta      480

caagaccacg cctcccgtgc tggactccga cggctccttc ttcctctata gcctgctcac      540

cgtggacaag agcaggtggc agcaggggaa cgtcttctca tgctccgtga tgcatgaggc      600

tctgcacaac cactacacgc agaagagcct ctccctgtct ccgggtaaat gagtttaacg      660

gatcttaatt aatccgagct cggtaccaag cttaag                                696


<210>  12
<211>  695
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CH2-3_DE_K360D-K409E

<400>  12
ccggaactcc tggggggacc gtcagtcttc ctcttccccc caaaacccaa ggacaccctc       60

atgatctccc ggacccctga ggtcacatgc gtggtggtgg acgtgagcca cgaagaccct      120

gaggtcaagt tcaactggta cgtggacggc gtggaggtgc ataatgccaa gacaaagccg      180

cgggaggagc agtacaacag cacgtaccgt gtggtcagcg tcctcaccgt cctgcaccag      240

gactggctga atggcaagga gtacaagtgc aaggtttcga acaaagccct cccagccccc      300

atcgagaaaa ccatctccaa agccaaaggg cagccccgag aaccacaggt gtacaccgac      360

cccccatccc gggaggagat gaccgacaac caggtcagcc tgacctgcga ggtcaaaggc      420

ttctatccca gcgacatcgc cgtggagtgg gagagcaatg ggcagccgga gaacaactac      480

aagaccacgc ctcccgtgct ggactccgac ggctccttct tcctctatag cgagctcacc      540

gtggacaaga gcaggtggca gcaggggaac gtcttctcat gctccgtgat gcatgaggct      600

ctgcacaacc actacacgca gaagagcctc tccctgtctc cgggtaaatg agtttaacgg      660

atcttaatta atccgagctc ggtaccaagc ttaag                                 695


<210>  13
<211>  696
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CH2-3_DE_S364T-K409E

<400>  13
tccggaactc ctggggggac cgtcagtctt cctcttcccc ccaaaaccca aggacaccct       60

catgatctcc cggacccctg aggtcacatg cgtggtggtg gacgtgagcc acgaagaccc      120

tgaggtcaag ttcaactggt acgtggacgg cgtggaggtg cataatgcca agacaaagcc      180

gcgggaggag cagtacaaca gcacgtaccg tgtggtcagc gtcctcaccg tcctgcacca      240

ggactggctg aatggcaagg agtacaagtg caaggtttcg aacaaagccc tcccagcccc      300

catcgagaaa accatctcca aagccaaagg gcagccccga gaaccacagg tgtacaccga      360

ccccccatcc cgggaggaga tgaccaagaa ccaggtcaca ctgacctgcg aggtcaaagg      420

cttctatccc agcgacatcg ccgtggagtg ggagagcaat gggcagccgg agaacaacta      480

caagaccacg cctcccgtgc tggactccga cggctccttc ttcctctata gcgagctcac      540

cgtggacaag agcaggtggc agcaggggaa cgtcttctca tgctccgtga tgcatgaggc      600

tctgcacaac cactacacgc agaagagcct ctccctgtct ccgggtaaat gagtttaacg      660

gatcttaatt aatccgagct cggtaccaag cttaag                                696


<210>  14
<211>  696
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CH2-3_DE_S364L-K409L

<400>  14
tccggaactc ctggggggac cgtcagtctt cctcttcccc ccaaaaccca aggacaccct       60

catgatctcc cggacccctg aggtcacatg cgtggtggtg gacgtgagcc acgaagaccc      120

tgaggtcaag ttcaactggt acgtggacgg cgtggaggtg cataatgcca agacaaagcc      180

gcgggaggag cagtacaaca gcacgtaccg tgtggtcagc gtcctcaccg tcctgcacca      240

ggactggctg aatggcaagg agtacaagtg caaggtttcg aacaaagccc tcccagcccc      300

catcgagaaa accatctcca aagccaaagg gcagccccga gaaccacagg tgtacaccga      360

ccccccatcc cgggaggaga tgaccaagaa ccaggtcctc ctgacctgcg aggtcaaagg      420

cttctatccc agcgacatcg ccgtggagtg ggagagcaat gggcagccgg agaacaacta      480

caagaccacg cctcccgtgc tggactccga cggctccttc ttcctctata gcctgctcac      540

cgtggacaag agcaggtggc agcaggggaa cgtcttctca tgctccgtga tgcatgaggc      600

tctgcacaac cactacacgc agaagagcct ctccctgtct ccgggtaaat gagtttaacg      660

gatcttaatt aatccgagct cggtaccaag cttaag                                696


<210>  15
<211>  696
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  MV1708_DE_K360D

<400>  15
tccggaactc ctggggggac cgtcagtctt cctcttcccc ccaaaaccca aggacaccct       60

catgatctcc cggacccctg aggtcacatg cgtggtggtg gacgtgagcc acgaagaccc      120

tgaggtcaag ttcaactggt acgtggacgg cgtggaggtg cataatgcca agacaaagcc      180

gcgggaggag cagtacaaca gcacgtaccg tgtggtcagc gtcctcaccg tcctgcacca      240

ggactggctg aatggcaagg agtacaagtg caaggtttcg aacaaagccc tcccagcccc      300

catcgagaaa accatctcca aagccaaagg gcagccccga gaaccacagg tgtacaccga      360

ccccccatcc cgggaggaga tgaccgacaa ccaggtcagc ctgacctgcg aggtcaaagg      420

cttctatccc agcgacatcg ccgtggagtg ggagagcaat gggcagccgg agaacaacta      480

caagaccacg cctcccgtgc tggactccga cggctccttc ttcctctata gcaagctcac      540

cgtggacaag agcaggtggc agcaggggaa cgtcttctca tgctccgtga tgcatgaggc      600

tctgcacaac cactacacgc agaagagcct ctccctgtct ccgggtaaat gagtttaacg      660

gatcttaatt aatccgagct cggtaccaag cttaag                                696


<210>  16
<211>  122
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VH sequence of anti-TT Fab

<400>  16

Glu Val Gln Leu Val Glu Thr Gly Gly Gly Val Val Gln Pro Gly Arg 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Gln Tyr 
            20                  25                  30          


Ala Met His Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val 
        35                  40                  45              


Ala Ile Ile Ser His Asp Glu Arg Asn Lys Tyr Tyr Val Asp Ser Gly 
    50                  55                  60                  


Met Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr Leu Phe 
65                  70                  75                  80  


Leu Gln Met Asn Ser Leu Arg Ser Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Asp Met Arg Lys Gly Gly Tyr Tyr Tyr Gly Phe Asp Val Trp 
            100                 105                 110         


Gly Gln Gly Thr Thr Val Thr Val Ser Ser 
        115                 120         


<210>  17
<211>  118
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VH sequence of anti-CD3 Fab

<400>  17
Gln Val Gln Leu Val Glu Ser Gly Gly Gly Val Val Gln Pro Gly Arg 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Arg Ser Tyr 
            20                  25                  30          


Gly Met His Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val 
        35                  40                  45              


Ala Ile Ile Trp Tyr Ser Gly Ser Lys Lys Asn Tyr Ala Asp Ser Val 
    50                  55                  60                  


Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr Leu Tyr 
65                  70                  75                  80  


Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Gly Thr Gly Tyr Asn Trp Phe Asp Pro Trp Gly Gln Gly Thr 
            100                 105                 110         


Leu Val Thr Val Ser Ser 
        115             

