               SEQUENCE LISTING

<110> GENETHON
      INSTITUT NATIONAL DE LA RECHERCHE ET DE LA SANTE MEDICALE
      UNIVERSITE EVRY VAL D’ESSONNE
      UNIVERSITE CLAUDE BERNARD LYON 1

<120> Vectors encoding a glucose-6-phosphatase (G6Pase-a) for gene therapy

<130> 1H274440_CAS 4

<150> EP20 305 540.5
<151> 2020-05-22

<160> 58

<170> BiSSAP 1.3.6

<210> 1
<211> 357
<212> PRT
<213> Homo sapiens


<220> 
<223> hG6Pase-a wt (amino acid)

<400> 1
Met Glu Glu Gly Met Asn Val Leu His Asp Phe Gly Ile Gln Ser Thr 
1               5                   10                  15      
His Tyr Leu Gln Val Asn Tyr Gln Asp Ser Gln Asp Trp Phe Ile Leu 
            20                  25                  30          
Val Ser Val Ile Ala Asp Leu Arg Asn Ala Phe Tyr Val Leu Phe Pro 
        35                  40                  45              
Ile Trp Phe His Leu Gln Glu Ala Val Gly Ile Lys Leu Leu Trp Val 
    50                  55                  60                  
Ala Val Ile Gly Asp Trp Leu Asn Leu Val Phe Lys Trp Ile Leu Phe 
65                  70                  75                  80  
Gly Gln Arg Pro Tyr Trp Trp Val Leu Asp Thr Asp Tyr Tyr Ser Asn 
                85                  90                  95      
Thr Ser Val Pro Leu Ile Lys Gln Phe Pro Val Thr Cys Glu Thr Gly 
            100                 105                 110         
Pro Gly Ser Pro Ser Gly His Ala Met Gly Thr Ala Gly Val Tyr Tyr 
        115                 120                 125             
Val Met Val Thr Ser Thr Leu Ser Ile Phe Gln Gly Lys Ile Lys Pro 
    130                 135                 140                 
Thr Tyr Arg Phe Arg Cys Leu Asn Val Ile Leu Trp Leu Gly Phe Trp 
145                 150                 155                 160 
Ala Val Gln Leu Asn Val Cys Leu Ser Arg Ile Tyr Leu Ala Ala His 
                165                 170                 175     
Phe Pro His Gln Val Val Ala Gly Val Leu Ser Gly Ile Ala Val Ala 
            180                 185                 190         
Glu Thr Phe Ser His Ile His Ser Ile Tyr Asn Ala Ser Leu Lys Lys 
        195                 200                 205             
Tyr Phe Leu Ile Thr Phe Phe Leu Phe Ser Phe Ala Ile Gly Phe Tyr 
    210                 215                 220                 
Leu Leu Leu Lys Gly Leu Gly Val Asp Leu Leu Trp Thr Leu Glu Lys 
225                 230                 235                 240 
Ala Gln Arg Trp Cys Glu Gln Pro Glu Trp Val His Ile Asp Thr Thr 
                245                 250                 255     
Pro Phe Ala Ser Leu Leu Lys Asn Leu Gly Thr Leu Phe Gly Leu Gly 
            260                 265                 270         
Leu Ala Leu Asn Ser Ser Met Tyr Arg Glu Ser Cys Lys Gly Lys Leu 
        275                 280                 285             
Ser Lys Trp Leu Pro Phe Arg Leu Ser Ser Ile Val Ala Ser Leu Val 
    290                 295                 300                 
Leu Leu His Val Phe Asp Ser Leu Lys Pro Pro Ser Gln Val Glu Leu 
305                 310                 315                 320 
Val Phe Tyr Val Leu Ser Phe Cys Lys Ser Ala Val Val Pro Leu Ala 
                325                 330                 335     
Ser Val Ser Val Ile Pro Tyr Cys Leu Ala Gln Val Leu Gly Gln Pro 
            340                 345                 350         
His Lys Lys Ser Leu 
        355         

<210> 2
<211> 1074
<212> DNA
<213> Homo sapiens


<220> 
<223> hG6pc wt (nucleic acid) (encoding SEQ ID NO:1)

<400> 2
atggaggaag gaatgaatgt tctccatgac tttgggatcc agtcaacaca ttacctccag      60

gtgaattacc aagactccca ggactggttc atcttggtgt ccgtgatcgc agacctcagg     120

aatgccttct acgtcctctt ccccatctgg ttccatcttc aggaagctgt gggcattaaa     180

ctcctttggg tagctgtgat tggagactgg ctcaacctcg tctttaagtg gattctcttt     240

ggacagcgtc catactggtg ggttttggat actgactact acagcaacac ttccgtgccc     300

ctgataaagc agttccctgt aacctgtgag actggaccag ggagcccctc tggccatgcc     360

atgggcacag caggtgtata ctacgtgatg gtcacatcta ctctttccat ctttcaggga     420

aagataaagc cgacctacag atttcggtgc ttgaatgtca ttttgtggtt gggattctgg     480

gctgtgcagc tgaatgtctg tctgtcacga atctaccttg ctgctcattt tcctcatcaa     540

gttgttgctg gagtcctgtc aggcattgct gttgcagaaa ctttcagcca catccacagc     600

atctataatg ccagcctcaa gaaatatttt ctcattacct tcttcctgtt cagcttcgcc     660

atcggatttt atctgctgct caagggactg ggtgtagacc tcctgtggac tctggagaaa     720

gcccagaggt ggtgcgagca gccagaatgg gtccacattg acaccacacc ctttgccagc     780

ctcctcaaga acctgggcac gctctttggc ctggggctgg ctctcaactc cagcatgtac     840

agggagagct gcaaggggaa actcagcaag tggctcccat tccgcctcag ctctattgta     900

gcctccctcg tcctcctgca cgtctttgac tccttgaaac ccccatccca agtcgagctg     960

gtcttctacg tcttgtcctt ctgcaagagt gcggtagtgc ccctggcatc cgtcagtgtc    1020

atcccctact gcctcgccca ggtcctgggc cagccgcaca agaagtcgtt gtaa          1074


<210> 3
<211> 1074
<212> DNA
<213> Artificial Sequence


<220> 
<223> hG6pc co 3 (nucleic acid) (Codon optimized - encoding SEQ ID NO :
      1 – used in Example 5)

<400> 3
atggaggagg gaatgaatgt gctgcacgac ttcgggattc agtctaccca ctatctccaa      60

gtcaattatc aagacagtca agattggttc atcctcgtct ccgttatagc tgacctcagg     120

aatgcttttt atgtactgtt cccaatatgg tttcatctgc aagaagcagt tggaattaaa     180

ctgctgtggg tggccgtaat cggagactgg ttgaacctgg tgttcaaatg gatccttttt     240

ggtcagaggc catattggtg ggtcttggac accgactatt atagcaacac atctgtaccc     300

ctgataaagc aattccctgt aacgtgtgaa actgggcctg ggtcacccag tggacacgcg     360

atggggactg ccggggttta ctacgtaatg gttactagca cgcttagcat tttccagggc     420

aagatcaaac cgacttatcg cttccgatgc ctcaatgtga tcctctggct gggattctgg     480

gcggttcaac tgaacgtatg cctctcacgg atttatctgg ctgcacattt cccgcatcaa     540

gtagtggctg gtgtgttgtc tggcatagca gtggctgaaa cattctcaca cattcattct     600

atttacaacg cttcattgaa aaaatacttt ctgattactt tttttctttt cagctttgca     660

attggctttt acttgctgct taaaggtctt ggcgtcgacc tgctctggac tcttgagaaa     720

gcgcaacgct ggtgcgaaca acccgagtgg gtccacatag atacgacccc gttcgcgtct     780

cttctgaaaa atctcggtac cctcttcgga ctgggacttg ctttgaattc ttcaatgtat     840

cgggaatcct gcaagggcaa actgtccaag tggttgccct ttcgcctttc aagcattgtc     900

gcttcccttg tgctcctgca cgtcttcgac tcactgaagc cgccgagcca agtcgagctc     960

gtgttttacg tgttgtcttt ctgtaaaagt gccgtcgtgc cactcgccag tgtgagcgtg    1020

ataccttatt gcctcgcaca ggtgcttggg cagcctcata agaagtctct gtaa          1074


<210> 4
<211> 1074
<212> DNA
<213> Artificial Sequence


<220> 
<223> hG6pc mut (nucleic acid) (identical to SEQ ID NO: 2 except for a
      c >g mutation at position 893) (Encoding SEQ ID NO : 12)

<400> 4
atggaggaag gaatgaatgt tctccatgac tttgggatcc agtcaacaca ttacctccag      60

gtgaattacc aagactccca ggactggttc atcttggtgt ccgtgatcgc agacctcagg     120

aatgccttct acgtcctctt ccccatctgg ttccatcttc aggaagctgt gggcattaaa     180

ctcctttggg tagctgtgat tggagactgg ctcaacctcg tctttaagtg gattctcttt     240

ggacagcgtc catactggtg ggttttggat actgactact acagcaacac ttccgtgccc     300

ctgataaagc agttccctgt aacctgtgag actggaccag ggagcccctc tggccatgcc     360

atgggcacag caggtgtata ctacgtgatg gtcacatcta ctctttccat ctttcaggga     420

aagataaagc cgacctacag atttcggtgc ttgaatgtca ttttgtggtt gggattctgg     480

gctgtgcagc tgaatgtctg tctgtcacga atctaccttg ctgctcattt tcctcatcaa     540

gttgttgctg gagtcctgtc aggcattgct gttgcagaaa ctttcagcca catccacagc     600

atctataatg ccagcctcaa gaaatatttt ctcattacct tcttcctgtt cagcttcgcc     660

atcggatttt atctgctgct caagggactg ggtgtagacc tcctgtggac tctggagaaa     720

gcccagaggt ggtgcgagca gccagaatgg gtccacattg acaccacacc ctttgccagc     780

ctcctcaaga acctgggcac gctctttggc ctggggctgg ctctcaactc cagcatgtac     840

agggagagct gcaaggggaa actcagcaag tggctcccat tccgcctcag ctgtattgta     900

gcctccctcg tcctcctgca cgtctttgac tccttgaaac ccccatccca agtcgagctg     960

gtcttctacg tcttgtcctt ctgcaagagt gcggtagtgc ccctggcatc cgtcagtgtc    1020

atcccctact gcctcgccca ggtcctgggc cagccgcaca agaagtcgtt gtaa          1074


<210> 5
<211> 1074
<212> DNA
<213> Artificial Sequence


<220> 
<223> hG6pc mut co (Nucleic acid)(Codon optimized - encoding SEQ ID NO
      : 12)

<400> 5
atggaggaag gaatgaatgt tctccatgac tttgggatcc agtcaacaca ttacctccag      60

gtgaattacc aagactccca ggactggttc atcttggtgt ccgtgatcgc agacctcagg     120

aatgccttct acgtcctctt ccccatctgg ttccatcttc aggaagctgt gggcattaaa     180

ctcctttggg tagctgtgat tggagactgg ctcaacctcg tctttaagtg gattctcttt     240

ggacagcgtc catactggtg ggttttggat actgactact acagcaacac ttccgtgccc     300

ctgataaagc agttccctgt aacctgtgag actggaccag ggagcccctc tggccatgcc     360

atgggcacag caggtgtata ctacgtgatg gtcacatcta ctctttccat ctttcaggga     420

aagataaagc cgacctacag atttcggtgc ttgaatgtca ttttgtggtt gggattctgg     480

gctgtgcagc tgaatgtctg tctgtcacga atctaccttg ctgctcattt tcctcatcaa     540

gttgttgctg gagtcctgtc aggcattgct gttgcagaaa ctttcagcca catccacagc     600

atctataatg ccagcctcaa gaaatatttt ctcattacct tcttcctgtt cagcttcgcc     660

atcggatttt atctgctgct caagggactg ggtgtagacc tcctgtggac tctggagaaa     720

gcccagaggt ggtgcgagca gccagaatgg gtccacattg acaccacacc ctttgccagc     780

ctcctcaaga acctgggcac gctctttggc ctggggctgg ctctcaactc cagcatgtac     840

agggagagct gcaaggggaa actcagcaag tggctcccat tccgcctcag ctgtattgta     900

gcctccctcg tcctcctgca cgtctttgac tccttgaaac ccccatccca agtcgagctg     960

gtcttctacg tcttgtcctt ctgcaagagt gcggtagtgc ccctggcatc cgtcagtgtc    1020

atcccctact gcctcgccca ggtcctgggc cagccgcaca agaagtcgtt gtaa          1074


<210> 6
<211> 2700
<212> DNA
<213> Artificial Sequence


<220> 
<223> hGPE promoter (4 nucleotides difference to hGPE promoter in
      NG011808.1)

<400> 6
tggaggaagc agaaaggggc tggcaggtgg aaagatgagg accagctcat cgtctcatga      60

ctatgaggtt gctctgatcc agaggtgccc cctgcctggt ggcccaccgc caggaagact     120

cccactgtcc ctggatgccc agagtgggat gtcaactcca tcacttatca actccttatc     180

cataggggta ttcttcctga ggcgtctcag aaaacagggc cctccccata tgctgaccac     240

ataatagaac ccctcccaac tcagagaccc tggctgctag ctgccctggc atgacccaga     300

cagtggcctt tgtatatgtt tttagactca ccttgactca cctctgacca tagaaactct     360

catcccagag gtcactgcaa tagttactcc acaacagagg cttatctggg tagagggagg     420

ctccctacct atggcccagc agccctgaca gtgcagatca catatacccc acgccccagc     480

actgcctgcc acgcatgggc ttactttaca cccacccaca gtcaccaaca cattacctgc     540

tctccaaggt taggcgtggc aggagaagtt tgcttggacc agcagaaacc atgcagtcaa     600

ggacaactgg agtcagcatg ggctgggtgc gagcccttgg tggggtgggg aggagactcc     660

aggtcatacc tcctggagga tgttttaatc atttccagca tggaatgctg tcaacttttg     720

ccacagattc attagctctg agtttctttt ttctgtcccc agctacccct tacatgtcaa     780

tatggactta atgatgggaa attcaggcaa gtttttaaac attttattcc ccctggctct     840

tatcctcaaa aaatgcatga atttggaggc agtggctcat gcctgtaatc ccaatgcttt     900

gctaggttga ggcgggagga tcacttgaag ccaggaattt gagaccagcc tgggccgcat     960

agtgagaccc cgtttctaca aaaataaata aataaataat aaataatagt gatatgaagc    1020

atgattaaat agccctattt tttaaaatgc atgagttcgt tacctgattc attccctggt    1080

tcctttcaca gtcctccgtg acccaagtgt tagggttttg gtctctctac tatttgtagg    1140

ctgatatata gtatacacac acacacacac acacatatac acacacacag tgtatcttga    1200

gctttctttt gtatatctac acacatatgt ataagaaagc tcaagatata gaagcccttt    1260

ttcaaaaata actgaaagtt tcaaactctt taagtctcca gttaccattt tgctggtatt    1320

cttatttgga accatacatt catcatattg ttgcacagta agactataca ttcattattt    1380

tgcttaaacg tatgagttaa aacacttggc caggcatggt ggttcacacc tgtaatccca    1440

gagctttggg aagccaagac tggcagtact cttgagctca ggaattcaag accagcctgg    1500

gcaacatgga aaaaccccat ctctacaaaa gatagaaaaa ttagccaggc atggtggcgt    1560

gtgcctgtgg tcccagctac tcaggaggct gaggtgggag gatcacatta gcccaggagg    1620

ttgaggctgc agtgagccgt gattatgcca ctgcactcca gcctgggaga cagagtgaga    1680

ccctgtttca aaaaaaagag agagaaaatt taaaaaagaa aacaacacca agggctgtaa    1740

ctttaaggtc attaaatgaa ttaatcactg cattcaaaaa cgattacttt ctggccctaa    1800

gagacatgag gccaatacca ggaagggggt tgatctccca aaccagaggc agaccctaga    1860

ctctaataca gttaaggaaa gaccagcaag atgatagtcc ccaatacaat agaagttact    1920

atattttatt tgttgttttt cttttgtttt gttttgtttt gttttgtttt gttttagaga    1980

ctggggtctt gctcgattgc ccaggctgta gtgcagcggt gggacaatag ctcactgcag    2040

actccaactc ctgggctcaa gcaatcctcc tgcctcagcc tcctgaatag ctgggactac    2100

aagggtacac catcacacac accaaaacaa ttttttaaat ttttgtgtag aaacgagggt    2160

cttgctttgt tgcccaggct ggtctccaac tcctggcttc aagggatcct cccacctcag    2220

cctcccaaat tgctgggatt acaggtgtga gccaccacaa ccagccagaa ctttactaat    2280

tttaaaatta agaacttaaa acttgaatag ctagagcacc aagatttttc tttgtcccca    2340

aataagtgca gttgcaggca tagaaaatct gacatctttg caagaatcat cgtggatgta    2400

gactctgtcc tgtgtctctg gcctggtttc ggggaccagg agggcagacc cttgcactgc    2460

caagaagcat gccaaagtta atcattggcc ctgctgagta catggccgat caggctgttt    2520

ttgtgtgcct gtttttctat tttacgtaaa tcaccctgaa catgtttgca tcaacctact    2580

ggtgatgcac ctttgatcaa tacattttag acaaacgtgg tttttgagtc caaagatcag    2640

ggctgggttg acctgaatac tggatacagg gcatataaaa caggggcaag gcacagactc    2700


<210> 7
<211> 727
<212> DNA
<213> Artificial Sequence


<220> 
<223> ApoE enhancer + hAAT promoter

<400> 7
aggctcagag gcacacagga gtttctgggc tcaccctgcc cccttccaac ccctcagttc      60

ccatcctcca gcagctgttt gtgtgctgcc tctgaagtcc acactgaaca aacttcagcc     120

tactcatgtc cctaaaatgg gcaaacattg caagcagcaa acagcaaaca cacagccctc     180

cctgcctgct gaccttggag ctggggcaga ggtcagagac ctctctgggc ccatgccacc     240

tccaacatcc actcgacccc ttggaatttc ggtggagagg agcagaggtt gtcctggcgt     300

ggtttaggta gtgtgagagg ggtacccggg gatcttgcta ccagtggaac agccactaag     360

gattctgcag tgagagcaga gggccagcta agtggtactc tcccagagac tgtctgactc     420

acgccacccc ctccaccttg gacacaggac gctgtggttt ctgagccagg tacaatgact     480

cctttcggta agtgcagtgg aagctgtaca ctgcccaggc aaagcgtccg ggcagcgtag     540

gcgggcgact cagatcccag ccagtggact tagcccctgt ttgctcctcc gataactggg     600

gtgaccttgg ttaatattca ccagcagcct cccccgttgc ccctctggat ccactgctta     660

aatacggacg aggacagggc cctgtctcct cagcttcagg caccaccact gacctgggac     720

agtgaat                                                               727


<210> 8
<211> 406
<212> DNA
<213> Artificial Sequence


<220> 
<223> hAAT promoter

<400> 8
gtacccgggg atcttgctac cagtggaaca gccactaagg attctgcagt gagagcagag      60

ggccagctaa gtggtactct cccagagact gtctgactca cgccaccccc tccaccttgg     120

acacaggacg ctgtggtttc tgagccaggt acaatgactc ctttcggtaa gtgcagtgga     180

agctgtacac tgcccaggca aagcgtccgg gcagcgtagg cgggcgactc agatcccagc     240

cagtggactt agcccctgtt tgctcctccg ataactgggg tgaccttggt taatattcac     300

cagcagcctc ccccgttgcc cctctggatc cactgcttaa atacggacga ggacagggcc     360

ctgtctcctc agcttcaggc accaccactg acctgggaca gtgaat                    406


<210> 9
<211> 321
<212> DNA
<213> Artificial Sequence


<220> 
<223> ApoE enhancer

<400> 9
aggctcagag gcacacagga gtttctgggc tcaccctgcc cccttccaac ccctcagttc      60

ccatcctcca gcagctgttt gtgtgctgcc tctgaagtcc acactgaaca aacttcagcc     120

tactcatgtc cctaaaatgg gcaaacattg caagcagcaa acagcaaaca cacagccctc     180

cctgcctgct gaccttggag ctggggcaga ggtcagagac ctctctgggc ccatgccacc     240

tccaacatcc actcgacccc ttggaatttc ggtggagagg agcagaggtt gtcctggcgt     300

ggtttaggta gtgtgagagg g                                               321


<210> 10
<211> 4492
<212> DNA
<213> Artificial Sequence


<220> 
<223> hGPE_HBB2_hG6pcwt_bGH (expression cassette used in examples 1, 2
      and 3)

<400> 10
tggaggaagc agaaaggggc tggcaggtgg aaagatgagg accagctcat cgtctcatga       60

ctatgaggtt gctctgatcc agaggtgccc cctgcctggt ggcccaccgc caggaagact      120

cccactgtcc ctggatgccc agagtgggat gtcaactcca tcacttatca actccttatc      180

cataggggta ttcttcctga ggcgtctcag aaaacagggc cctccccata tgctgaccac      240

ataatagaac ccctcccaac tcagagaccc tggctgctag ctgccctggc atgacccaga      300

cagtggcctt tgtatatgtt tttagactca ccttgactca cctctgacca tagaaactct      360

catcccagag gtcactgcaa tagttactcc acaacagagg cttatctggg tagagggagg      420

ctccctacct atggcccagc agccctgaca gtgcagatca catatacccc acgccccagc      480

actgcctgcc acgcatgggc ttactttaca cccacccaca gtcaccaaca cattacctgc      540

tctccaaggt taggcgtggc aggagaagtt tgcttggacc agcagaaacc atgcagtcaa      600

ggacaactgg agtcagcatg ggctgggtgc gagcccttgg tggggtgggg aggagactcc      660

aggtcatacc tcctggagga tgttttaatc atttccagca tggaatgctg tcaacttttg      720

ccacagattc attagctctg agtttctttt ttctgtcccc agctacccct tacatgtcaa      780

tatggactta atgatgggaa attcaggcaa gtttttaaac attttattcc ccctggctct      840

tatcctcaaa aaatgcatga atttggaggc agtggctcat gcctgtaatc ccaatgcttt      900

gctaggttga ggcgggagga tcacttgaag ccaggaattt gagaccagcc tgggccgcat      960

agtgagaccc cgtttctaca aaaataaata aataaataat aaataatagt gatatgaagc     1020

atgattaaat agccctattt tttaaaatgc atgagttcgt tacctgattc attccctggt     1080

tcctttcaca gtcctccgtg acccaagtgt tagggttttg gtctctctac tatttgtagg     1140

ctgatatata gtatacacac acacacacac acacatatac acacacacag tgtatcttga     1200

gctttctttt gtatatctac acacatatgt ataagaaagc tcaagatata gaagcccttt     1260

ttcaaaaata actgaaagtt tcaaactctt taagtctcca gttaccattt tgctggtatt     1320

cttatttgga accatacatt catcatattg ttgcacagta agactataca ttcattattt     1380

tgcttaaacg tatgagttaa aacacttggc caggcatggt ggttcacacc tgtaatccca     1440

gagctttggg aagccaagac tggcagtact cttgagctca ggaattcaag accagcctgg     1500

gcaacatgga aaaaccccat ctctacaaaa gatagaaaaa ttagccaggc atggtggcgt     1560

gtgcctgtgg tcccagctac tcaggaggct gaggtgggag gatcacatta gcccaggagg     1620

ttgaggctgc agtgagccgt gattatgcca ctgcactcca gcctgggaga cagagtgaga     1680

ccctgtttca aaaaaaagag agagaaaatt taaaaaagaa aacaacacca agggctgtaa     1740

ctttaaggtc attaaatgaa ttaatcactg cattcaaaaa cgattacttt ctggccctaa     1800

gagacatgag gccaatacca ggaagggggt tgatctccca aaccagaggc agaccctaga     1860

ctctaataca gttaaggaaa gaccagcaag atgatagtcc ccaatacaat agaagttact     1920

atattttatt tgttgttttt cttttgtttt gttttgtttt gttttgtttt gttttagaga     1980

ctggggtctt gctcgattgc ccaggctgta gtgcagcggt gggacaatag ctcactgcag     2040

actccaactc ctgggctcaa gcaatcctcc tgcctcagcc tcctgaatag ctgggactac     2100

aagggtacac catcacacac accaaaacaa ttttttaaat ttttgtgtag aaacgagggt     2160

cttgctttgt tgcccaggct ggtctccaac tcctggcttc aagggatcct cccacctcag     2220

cctcccaaat tgctgggatt acaggtgtga gccaccacaa ccagccagaa ctttactaat     2280

tttaaaatta agaacttaaa acttgaatag ctagagcacc aagatttttc tttgtcccca     2340

aataagtgca gttgcaggca tagaaaatct gacatctttg caagaatcat cgtggatgta     2400

gactctgtcc tgtgtctctg gcctggtttc ggggaccagg agggcagacc cttgcactgc     2460

caagaagcat gccaaagtta atcattggcc ctgctgagta catggccgat caggctgttt     2520

ttgtgtgcct gtttttctat tttacgtaaa tcaccctgaa catgtttgca tcaacctact     2580

ggtgatgcac ctttgatcaa tacattttag acaaacgtgg tttttgagtc caaagatcag     2640

ggctgggttg acctgaatac tggatacagg gcatataaaa caggggcaag gcacagactc     2700

gggacccttg atgttttctt tccccttctt ttctatggtt aagttcatgt cataggaagg     2760

ggagaagtaa cagggtacac atattgacca aatcagggta attttgcatt tgtaatttta     2820

aaaaatgctt tcttctttta atatactttt ttgtttatct tatttctaat actttcccta     2880

atctctttct ttcagggcaa tattgataca atgtatcttg cctctttgca ccattctaaa     2940

gaataacagt gataatttct gggttaaggc aatagcaata tttctgcata taaatatttc     3000

tgcatataaa ttgtaactga tgtaagaggt ttcatattgc taatagcagc tacaatccag     3060

ctaccattct gcttttattt tctggttggg ataaggctgg attattctga gtccaagcta     3120

ggcccttttg ctaatcttgt tcatacctct tatcttcctc ccacagctcc tgggcaacct     3180

gctggtctct ctgctggccc atcactttgg caaagcacgc gtgccaccat ggaggaagga     3240

atgaatgttc tccatgactt tgggatccag tcaacacatt acctccaggt gaattaccaa     3300

gactcccagg actggttcat cttggtgtcc gtgatcgcag acctcaggaa tgccttctac     3360

gtcctcttcc ccatctggtt ccatcttcag gaagctgtgg gcattaaact cctttgggta     3420

gctgtgattg gagactggct caacctcgtc tttaagtgga ttctctttgg acagcgtcca     3480

tactggtggg ttttggatac tgactactac agcaacactt ccgtgcccct gataaagcag     3540

ttccctgtaa cctgtgagac tggaccaggg agcccctctg gccatgccat gggcacagca     3600

ggtgtatact acgtgatggt cacatctact ctttccatct ttcagggaaa gataaagccg     3660

acctacagat ttcggtgctt gaatgtcatt ttgtggttgg gattctgggc tgtgcagctg     3720

aatgtctgtc tgtcacgaat ctaccttgct gctcattttc ctcatcaagt tgttgctgga     3780

gtcctgtcag gcattgctgt tgcagaaact ttcagccaca tccacagcat ctataatgcc     3840

agcctcaaga aatattttct cattaccttc ttcctgttca gcttcgccat cggattttat     3900

ctgctgctca agggactggg tgtagacctc ctgtggactc tggagaaagc ccagaggtgg     3960

tgcgagcagc cagaatgggt ccacattgac accacaccct ttgccagcct cctcaagaac     4020

ctgggcacgc tctttggcct ggggctggct ctcaactcca gcatgtacag ggagagctgc     4080

aaggggaaac tcagcaagtg gctcccattc cgcctcagct ctattgtagc ctccctcgtc     4140

ctcctgcacg tctttgactc cttgaaaccc ccatcccaag tcgagctggt cttctacgtc     4200

ttgtccttct gcaagagtgc ggtagtgccc ctggcatccg tcagtgtcat cccctactgc     4260

ctcgcccagg tcctgggcca gccgcacaag aagtcgttgt aaagatctga attcaccgcg     4320

ggtttaaact gtgccttcta gttgccagcc atctgttgtt tgcccctccc ccgtgccttc     4380

cttgaccctg gaaggtgcca ctcccactgt cctttcctaa taaaatgagg aaattgcatc     4440

gcattgtctg agtaggtgtc attctattct ggggggtggg gtgggggcta gc             4492


<210> 11
<211> 3153
<212> DNA
<213> Artificial Sequence


<220> 
<223> hAAT_HBB2_hG6pc wt (expression cassette used in examples 1, 2, 3,
      4 and 5)

<400> 11
ggatcaaggc tcagaggcac acaggagttt ctgggctcac cctgccccct tccaacccct      60

cagttcccat cctccagcag ctgtttgtgt gctgcctctg aagtccacac tgaacaaact     120

tcagcctact catgtcccta aaatgggcaa acattgcaag cagcaaacag caaacacaca     180

gccctccctg cctgctgacc ttggagctgg ggcagaggtc agagacctct ctgggcccat     240

gccacctcca acatccactc gaccccttgg aatttcggtg gagaggagca gaggttgtcc     300

tggcgtggtt taggtagtgt gagaggggta cccggggatc ttgctaccag tggaacagcc     360

actaaggatt ctgcagtgag agcagagggc cagctaagtg gtactctccc agagactgtc     420

tgactcacgc caccccctcc accttggaca caggacgctg tggtttctga gccaggtaca     480

atgactcctt tcggtaagtg cagtggaagc tgtacactgc ccaggcaaag cgtccgggca     540

gcgtaggcgg gcgactcaga tcccagccag tggacttagc ccctgtttgc tcctccgata     600

actggggtga ccttggttaa tattcaccag cagcctcccc cgttgcccct ctggatccac     660

tgcttaaata cggacgagga cagggccctg tctcctcagc ttcaggcacc accactgacc     720

tgggacagtg aatagatcct gagaacttca gggtgagtct atgggaccct tgatgttttc     780

tttccccttc ttttctatgg ttaagttcat gtcataggaa ggggagaagt aacagggtac     840

acatattgac caaatcaggg taattttgca tttgtaattt taaaaaatgc tttcttcttt     900

taatatactt ttttgtttat cttatttcta atactttccc taatctcttt ctttcagggc     960

aatattgata caatgtatct tgcctctttg caccattcta aagaataaca gtgataattt    1020

ctgggttaag gcaatagcaa tatttctgca tataaatatt tctgcatata aattgtaact    1080

gatgtaagag gtttcatatt gctaatagca gctacaatcc agctaccatt ctgcttttat    1140

tttctggttg ggataaggct ggattattct gagtccaagc taggcccttt tgctaatctt    1200

gttcatacct cttatcttcc tcccacagct cctgggcaac ctgctggtct ctctgctggc    1260

ccatcacttt ggcaaagcac gcgtgccacc atggaggaag gaatgaatgt tctccatgac    1320

tttgggatcc agtcaacaca ttacctccag gtgaattacc aagactccca ggactggttc    1380

atcttggtgt ccgtgatcgc agacctcagg aatgccttct acgtcctctt ccccatctgg    1440

ttccatcttc aggaagctgt gggcattaaa ctcctttggg tagctgtgat tggagactgg    1500

ctcaacctcg tctttaagtg gattctcttt ggacagcgtc catactggtg ggttttggat    1560

actgactact acagcaacac ttccgtgccc ctgataaagc agttccctgt aacctgtgag    1620

actggaccag ggagcccctc tggccatgcc atgggcacag caggtgtata ctacgtgatg    1680

gtcacatcta ctctttccat ctttcaggga aagataaagc cgacctacag atttcggtgc    1740

ttgaatgtca ttttgtggtt gggattctgg gctgtgcagc tgaatgtctg tctgtcacga    1800

atctaccttg ctgctcattt tcctcatcaa gttgttgctg gagtcctgtc aggcattgct    1860

gttgcagaaa ctttcagcca catccacagc atctataatg ccagcctcaa gaaatatttt    1920

ctcattacct tcttcctgtt cagcttcgcc atcggatttt atctgctgct caagggactg    1980

ggtgtagacc tcctgtggac tctggagaaa gcccagaggt ggtgcgagca gccagaatgg    2040

gtccacattg acaccacacc ctttgccagc ctcctcaaga acctgggcac gctctttggc    2100

ctggggctgg ctctcaactc cagcatgtac agggagagct gcaaggggaa actcagcaag    2160

tggctcccat tccgcctcag ctctattgta gcctccctcg tcctcctgca cgtctttgac    2220

tccttgaaac ccccatccca agtcgagctg gtcttctacg tcttgtcctt ctgcaagagt    2280

gcggtagtgc ccctggcatc cgtcagtgtc atcccctact gcctcgccca ggtcctgggc    2340

cagccgcaca agaagtcgtt gtaaagatct gaattcaccc caccagtgca ggctgcctat    2400

cagaaagtgg tggctggtgt ggctaatgcc ctggcccaca agtatcacta agctcgcttt    2460

cttgctgtcc aatttctatt aaaggttcct ttgttcccta agtccaacta ctaaactggg    2520

ggatattatg aagggccttg agcatctgga ttctgcctaa taaaaaacat ttattttcat    2580

tgcaatgatg tatttaaatt atttctgaat attttactaa aaagggaatg tgggaggtca    2640

gtgcatttaa aacataaaga aatgaagagc tagttcaaac cttgggaaaa tacactatat    2700

cttaaactcc atgaaagaag gtgaggctgc aaacagctaa tgcacattgg caacagcccc    2760

tgatgcctat gccttattca tccctcagaa aaggattcaa gtagaggctt gatttggagg    2820

ttaaagtttg gctatgctgt attttacatt acttattgtt ttagctgtcc tcatgaatgt    2880

cttttcacta cccatttgct tatcctgcat ctctcagcct tgactccact cagttctctt    2940

gcttagagat accacctttc ccctgaagtg ttccttccat gttttacggc gagatggttt    3000

ctcctcgcct ggccactcag ccttagttgt ctctgttgtc ttatagaggt ctacttgaag    3060

aaggaaaaac agggggcatg gtttgactgt cctgtgagcc cttcttccct gcctccccca    3120

ctcacagtga cccggaatcc ctcgacatgg cag                                 3153


<210> 12
<211> 357
<212> PRT
<213> Artificial Sequence


<220> 
<223> hG6Pase-a mut (amino acid)

<400> 12
Met Glu Glu Gly Met Asn Val Leu His Asp Phe Gly Ile Gln Ser Thr 
1               5                   10                  15      
His Tyr Leu Gln Val Asn Tyr Gln Asp Ser Gln Asp Trp Phe Ile Leu 
            20                  25                  30          
Val Ser Val Ile Ala Asp Leu Arg Asn Ala Phe Tyr Val Leu Phe Pro 
        35                  40                  45              
Ile Trp Phe His Leu Gln Glu Ala Val Gly Ile Lys Leu Leu Trp Val 
    50                  55                  60                  
Ala Val Ile Gly Asp Trp Leu Asn Leu Val Phe Lys Trp Ile Leu Phe 
65                  70                  75                  80  
Gly Gln Arg Pro Tyr Trp Trp Val Leu Asp Thr Asp Tyr Tyr Ser Asn 
                85                  90                  95      
Thr Ser Val Pro Leu Ile Lys Gln Phe Pro Val Thr Cys Glu Thr Gly 
            100                 105                 110         
Pro Gly Ser Pro Ser Gly His Ala Met Gly Thr Ala Gly Val Tyr Tyr 
        115                 120                 125             
Val Met Val Thr Ser Thr Leu Ser Ile Phe Gln Gly Lys Ile Lys Pro 
    130                 135                 140                 
Thr Tyr Arg Phe Arg Cys Leu Asn Val Ile Leu Trp Leu Gly Phe Trp 
145                 150                 155                 160 
Ala Val Gln Leu Asn Val Cys Leu Ser Arg Ile Tyr Leu Ala Ala His 
                165                 170                 175     
Phe Pro His Gln Val Val Ala Gly Val Leu Ser Gly Ile Ala Val Ala 
            180                 185                 190         
Glu Thr Phe Ser His Ile His Ser Ile Tyr Asn Ala Ser Leu Lys Lys 
        195                 200                 205             
Tyr Phe Leu Ile Thr Phe Phe Leu Phe Ser Phe Ala Ile Gly Phe Tyr 
    210                 215                 220                 
Leu Leu Leu Lys Gly Leu Gly Val Asp Leu Leu Trp Thr Leu Glu Lys 
225                 230                 235                 240 
Ala Gln Arg Trp Cys Glu Gln Pro Glu Trp Val His Ile Asp Thr Thr 
                245                 250                 255     
Pro Phe Ala Ser Leu Leu Lys Asn Leu Gly Thr Leu Phe Gly Leu Gly 
            260                 265                 270         
Leu Ala Leu Asn Ser Ser Met Tyr Arg Glu Ser Cys Lys Gly Lys Leu 
        275                 280                 285             
Ser Lys Trp Leu Pro Phe Arg Leu Ser Cys Ile Val Ala Ser Leu Val 
    290                 295                 300                 
Leu Leu His Val Phe Asp Ser Leu Lys Pro Pro Ser Gln Val Glu Leu 
305                 310                 315                 320 
Val Phe Tyr Val Leu Ser Phe Cys Lys Ser Ala Val Val Pro Leu Ala 
                325                 330                 335     
Ser Val Ser Val Ile Pro Tyr Cys Leu Ala Gln Val Leu Gly Gln Pro 
            340                 345                 350         
His Lys Lys Ser Leu 
        355         

<210> 13
<211> 1074
<212> DNA
<213> Artificial Sequence


<220> 
<223> Nucleic acid coding modified G6Pase-a (R3K)

<400> 13
atggagaaag gaatgaatgt tctccatgac tttgggatcc agtcaacaca ttacctccag      60

gtgaattacc aagactccca ggactggttc atcttggtgt ccgtgatcgc agacctcagg     120

aatgccttct acgtcctctt ccccatctgg ttccatcttc aggaagctgt gggcattaaa     180

ctcctttggg tagctgtgat tggagactgg ctcaacctcg tctttaagtg gattctcttt     240

ggacagcgtc catactggtg ggttttggat actgactact acagcaacac ttccgtgccc     300

ctgataaagc agttccctgt aacctgtgag actggaccag ggagcccctc tggccatgcc     360

atgggcacag caggtgtata ctacgtgatg gtcacatcta ctctttccat ctttcaggga     420

aagataaagc cgacctacag atttcggtgc ttgaatgtca ttttgtggtt gggattctgg     480

gctgtgcagc tgaatgtctg tctgtcacga atctaccttg ctgctcattt tcctcatcaa     540

gttgttgctg gagtcctgtc aggcattgct gttgcagaaa ctttcagcca catccacagc     600

atctataatg ccagcctcaa gaaatatttt ctcattacct tcttcctgtt cagcttcgcc     660

atcggatttt atctgctgct caagggactg ggtgtagacc tcctgtggac tctggagaaa     720

gcccagaggt ggtgcgagca gccagaatgg gtccacattg acaccacacc ctttgccagc     780

ctcctcaaga acctgggcac gctctttggc ctggggctgg ctctcaactc cagcatgtac     840

agggagagct gcaaggggaa actcagcaag tggctcccat tccgcctcag ctctattgta     900

gcctccctcg tcctcctgca cgtctttgac tccttgaaac ccccatccca agtcgagctg     960

gtcttctacg tcttgtcctt ctgcaagagt gcggtagtgc ccctggcatc cgtcagtgtc    1020

atcccctact gcctcgccca ggtcctgggc cagccgcaca agaagtcgtt gtaa          1074


<210> 14
<211> 1074
<212> DNA
<213> Artificial Sequence


<220> 
<223> Nucleic acid coding modified G6Pase-a (Q54R)

<400> 14
atggaggaag gaatgaatgt tctccatgac tttgggatcc agtcaacaca ttacctccag      60

gtgaattacc aagactccca ggactggttc atcttggtgt ccgtgatcgc agacctcagg     120

aatgccttct acgtcctctt ccccatctgg ttccatcttc gtgaagctgt gggcattaaa     180

ctcctttggg tagctgtgat tggagactgg ctcaacctcg tctttaagtg gattctcttt     240

ggacagcgtc catactggtg ggttttggat actgactact acagcaacac ttccgtgccc     300

ctgataaagc agttccctgt aacctgtgag actggaccag ggagcccctc tggccatgcc     360

atgggcacag caggtgtata ctacgtgatg gtcacatcta ctctttccat ctttcaggga     420

aagataaagc cgacctacag atttcggtgc ttgaatgtca ttttgtggtt gggattctgg     480

gctgtgcagc tgaatgtctg tctgtcacga atctaccttg ctgctcattt tcctcatcaa     540

gttgttgctg gagtcctgtc aggcattgct gttgcagaaa ctttcagcca catccacagc     600

atctataatg ccagcctcaa gaaatatttt ctcattacct tcttcctgtt cagcttcgcc     660

atcggatttt atctgctgct caagggactg ggtgtagacc tcctgtggac tctggagaaa     720

gcccagaggt ggtgcgagca gccagaatgg gtccacattg acaccacacc ctttgccagc     780

ctcctcaaga acctgggcac gctctttggc ctggggctgg ctctcaactc cagcatgtac     840

agggagagct gcaaggggaa actcagcaag tggctcccat tccgcctcag ctctattgta     900

gcctccctcg tcctcctgca cgtctttgac tccttgaaac ccccatccca agtcgagctg     960

gtcttctacg tcttgtcctt ctgcaagagt gcggtagtgc ccctggcatc cgtcagtgtc    1020

atcccctact gcctcgccca ggtcctgggc cagccgcaca agaagtcgtt gtaa          1074


<210> 15
<211> 1074
<212> DNA
<213> Artificial Sequence


<220> 
<223> Nucleic acid coding modified G6Pase-a (Q139R)

<400> 15
atggaggaag gaatgaatgt tctccatgac tttgggatcc agtcaacaca ttacctccag      60

gtgaattacc aagactccca ggactggttc atcttggtgt ccgtgatcgc agacctcagg     120

aatgccttct acgtcctctt ccccatctgg ttccatcttc aggaagctgt gggcattaaa     180

ctcctttggg tagctgtgat tggagactgg ctcaacctcg tctttaagtg gattctcttt     240

ggacagcgtc catactggtg ggttttggat actgactact acagcaacac ttccgtgccc     300

ctgataaagc agttccctgt aacctgtgag actggaccag ggagcccctc tggccatgcc     360

atgggcacag caggtgtata ctacgtgatg gtcacatcta ctctttccat ctttcgggga     420

aagataaagc cgacctacag atttcggtgc ttgaatgtca ttttgtggtt gggattctgg     480

gctgtgcagc tgaatgtctg tctgtcacga atctaccttg ctgctcattt tcctcatcaa     540

gttgttgctg gagtcctgtc aggcattgct gttgcagaaa ctttcagcca catccacagc     600

atctataatg ccagcctcaa gaaatatttt ctcattacct tcttcctgtt cagcttcgcc     660

atcggatttt atctgctgct caagggactg ggtgtagacc tcctgtggac tctggagaaa     720

gcccagaggt ggtgcgagca gccagaatgg gtccacattg acaccacacc ctttgccagc     780

ctcctcaaga acctgggcac gctctttggc ctggggctgg ctctcaactc cagcatgtac     840

agggagagct gcaaggggaa actcagcaag tggctcccat tccgcctcag ctctattgta     900

gcctccctcg tcctcctgca cgtctttgac tccttgaaac ccccatccca agtcgagctg     960

gtcttctacg tcttgtcctt ctgcaagagt gcggtagtgc ccctggcatc cgtcagtgtc    1020

atcccctact gcctcgccca ggtcctgggc cagccgcaca agaagtcgtt gtaa          1074


<210> 16
<211> 1074
<212> DNA
<213> Artificial Sequence


<220> 
<223> Nucleic acid coding modified G6Pase-a (I142K)

<400> 16
atggaggaag gaatgaatgt tctccatgac tttgggatcc agtcaacaca ttacctccag      60

gtgaattacc aagactccca ggactggttc atcttggtgt ccgtgatcgc agacctcagg     120

aatgccttct acgtcctctt ccccatctgg ttccatcttc aggaagctgt gggcattaaa     180

ctcctttggg tagctgtgat tggagactgg ctcaacctcg tctttaagtg gattctcttt     240

ggacagcgtc catactggtg ggttttggat actgactact acagcaacac ttccgtgccc     300

ctgataaagc agttccctgt aacctgtgag actggaccag ggagcccctc tggccatgcc     360

atgggcacag caggtgtata ctacgtgatg gtcacatcta ctctttccat ctttcaggga     420

aagaaaaagc cgacctacag atttcggtgc ttgaatgtca ttttgtggtt gggattctgg     480

gctgtgcagc tgaatgtctg tctgtcacga atctaccttg ctgctcattt tcctcatcaa     540

gttgttgctg gagtcctgtc aggcattgct gttgcagaaa ctttcagcca catccacagc     600

atctataatg ccagcctcaa gaaatatttt ctcattacct tcttcctgtt cagcttcgcc     660

atcggatttt atctgctgct caagggactg ggtgtagacc tcctgtggac tctggagaaa     720

gcccagaggt ggtgcgagca gccagaatgg gtccacattg acaccacacc ctttgccagc     780

ctcctcaaga acctgggcac gctctttggc ctggggctgg ctctcaactc cagcatgtac     840

agggagagct gcaaggggaa actcagcaag tggctcccat tccgcctcag ctctattgta     900

gcctccctcg tcctcctgca cgtctttgac tccttgaaac ccccatccca agtcgagctg     960

gtcttctacg tcttgtcctt ctgcaagagt gcggtagtgc ccctggcatc cgtcagtgtc    1020

atcccctact gcctcgccca ggtcctgggc cagccgcaca agaagtcgtt gtaa          1074


<210> 17
<211> 1074
<212> DNA
<213> Artificial Sequence


<220> 
<223> Nucleic acid coding modified G6Pase-a (S196R)

<400> 17
atggaggaag gaatgaatgt tctccatgac tttgggatcc agtcaacaca ttacctccag      60

gtgaattacc aagactccca ggactggttc atcttggtgt ccgtgatcgc agacctcagg     120

aatgccttct acgtcctctt ccccatctgg ttccatcttc aggaagctgt gggcattaaa     180

ctcctttggg tagctgtgat tggagactgg ctcaacctcg tctttaagtg gattctcttt     240

ggacagcgtc catactggtg ggttttggat actgactact acagcaacac ttccgtgccc     300

ctgataaagc agttccctgt aacctgtgag actggaccag ggagcccctc tggccatgcc     360

atgggcacag caggtgtata ctacgtgatg gtcacatcta ctctttccat ctttcaggga     420

aagataaagc cgacctacag atttcggtgc ttgaatgtca ttttgtggtt gggattctgg     480

gctgtgcagc tgaatgtctg tctgtcacga atctaccttg ctgctcattt tcctcatcaa     540

gttgttgctg gagtcctgtc aggcattgct gttgcagaaa ctttccgcca catccacagc     600

atctataatg ccagcctcaa gaaatatttt ctcattacct tcttcctgtt cagcttcgcc     660

atcggatttt atctgctgct caagggactg ggtgtagacc tcctgtggac tctggagaaa     720

gcccagaggt ggtgcgagca gccagaatgg gtccacattg acaccacacc ctttgccagc     780

ctcctcaaga acctgggcac gctctttggc ctggggctgg ctctcaactc cagcatgtac     840

agggagagct gcaaggggaa actcagcaag tggctcccat tccgcctcag ctctattgta     900

gcctccctcg tcctcctgca cgtctttgac tccttgaaac ccccatccca agtcgagctg     960

gtcttctacg tcttgtcctt ctgcaagagt gcggtagtgc ccctggcatc cgtcagtgtc    1020

atcccctact gcctcgccca ggtcctgggc cagccgcaca agaagtcgtt gtaa          1074


<210> 18
<211> 1074
<212> DNA
<213> Artificial Sequence


<220> 
<223> Nucleic acid coding modified G6Pase-a (H199Q)

<400> 18
atggaggaag gaatgaatgt tctccatgac tttgggatcc agtcaacaca ttacctccag      60

gtgaattacc aagactccca ggactggttc atcttggtgt ccgtgatcgc agacctcagg     120

aatgccttct acgtcctctt ccccatctgg ttccatcttc aggaagctgt gggcattaaa     180

ctcctttggg tagctgtgat tggagactgg ctcaacctcg tctttaagtg gattctcttt     240

ggacagcgtc catactggtg ggttttggat actgactact acagcaacac ttccgtgccc     300

ctgataaagc agttccctgt aacctgtgag actggaccag ggagcccctc tggccatgcc     360

atgggcacag caggtgtata ctacgtgatg gtcacatcta ctctttccat ctttcaggga     420

aagataaagc cgacctacag atttcggtgc ttgaatgtca ttttgtggtt gggattctgg     480

gctgtgcagc tgaatgtctg tctgtcacga atctaccttg ctgctcattt tcctcatcaa     540

gttgttgctg gagtcctgtc aggcattgct gttgcagaaa ctttcagcca catccagagc     600

atctataatg ccagcctcaa gaaatatttt ctcattacct tcttcctgtt cagcttcgcc     660

atcggatttt atctgctgct caagggactg ggtgtagacc tcctgtggac tctggagaaa     720

gcccagaggt ggtgcgagca gccagaatgg gtccacattg acaccacacc ctttgccagc     780

ctcctcaaga acctgggcac gctctttggc ctggggctgg ctctcaactc cagcatgtac     840

agggagagct gcaaggggaa actcagcaag tggctcccat tccgcctcag ctctattgta     900

gcctccctcg tcctcctgca cgtctttgac tccttgaaac ccccatccca agtcgagctg     960

gtcttctacg tcttgtcctt ctgcaagagt gcggtagtgc ccctggcatc cgtcagtgtc    1020

atcccctact gcctcgccca ggtcctgggc cagccgcaca agaagtcgtt gtaa          1074


<210> 19
<211> 1074
<212> DNA
<213> Artificial Sequence


<220> 
<223> Nucleic acid coding modified G6Pase-a (Q242R)

<400> 19
atggaggaag gaatgaatgt tctccatgac tttgggatcc agtcaacaca ttacctccag      60

gtgaattacc aagactccca ggactggttc atcttggtgt ccgtgatcgc agacctcagg     120

aatgccttct acgtcctctt ccccatctgg ttccatcttc aggaagctgt gggcattaaa     180

ctcctttggg tagctgtgat tggagactgg ctcaacctcg tctttaagtg gattctcttt     240

ggacagcgtc catactggtg ggttttggat actgactact acagcaacac ttccgtgccc     300

ctgataaagc agttccctgt aacctgtgag actggaccag ggagcccctc tggccatgcc     360

atgggcacag caggtgtata ctacgtgatg gtcacatcta ctctttccat ctttcaggga     420

aagataaagc cgacctacag atttcggtgc ttgaatgtca ttttgtggtt gggattctgg     480

gctgtgcagc tgaatgtctg tctgtcacga atctaccttg ctgctcattt tcctcatcaa     540

gttgttgctg gagtcctgtc aggcattgct gttgcagaaa ctttcagcca catccacagc     600

atctataatg ccagcctcaa gaaatatttt ctcattacct tcttcctgtt cagcttcgcc     660

atcggatttt atctgctgct caagggactg ggtgtagacc tcctgtggac tctggagaaa     720

gccaggaggt ggtgcgagca gccagaatgg gtccacattg acaccacacc ctttgccagc     780

ctcctcaaga acctgggcac gctctttggc ctggggctgg ctctcaactc cagcatgtac     840

agggagagct gcaaggggaa actcagcaag tggctcccat tccgcctcag ctctattgta     900

gcctccctcg tcctcctgca cgtctttgac tccttgaaac ccccatccca agtcgagctg     960

gtcttctacg tcttgtcctt ctgcaagagt gcggtagtgc ccctggcatc cgtcagtgtc    1020

atcccctact gcctcgccca ggtcctgggc cagccgcaca agaagtcgtt gtaa          1074


<210> 20
<211> 1074
<212> DNA
<213> Artificial Sequence


<220> 
<223> Nucleic acid coding modified G6Pase-a (Q247R)

<400> 20
atggaggaag gaatgaatgt tctccatgac tttgggatcc agtcaacaca ttacctccag      60

gtgaattacc aagactccca ggactggttc atcttggtgt ccgtgatcgc agacctcagg     120

aatgccttct acgtcctctt ccccatctgg ttccatcttc aggaagctgt gggcattaaa     180

ctcctttggg tagctgtgat tggagactgg ctcaacctcg tctttaagtg gattctcttt     240

ggacagcgtc catactggtg ggttttggat actgactact acagcaacac ttccgtgccc     300

ctgataaagc agttccctgt aacctgtgag actggaccag ggagcccctc tggccatgcc     360

atgggcacag caggtgtata ctacgtgatg gtcacatcta ctctttccat ctttcaggga     420

aagataaagc cgacctacag atttcggtgc ttgaatgtca ttttgtggtt gggattctgg     480

gctgtgcagc tgaatgtctg tctgtcacga atctaccttg ctgctcattt tcctcatcaa     540

gttgttgctg gagtcctgtc aggcattgct gttgcagaaa ctttcagcca catccacagc     600

atctataatg ccagcctcaa gaaatatttt ctcattacct tcttcctgtt cagcttcgcc     660

atcggatttt atctgctgct caagggactg ggtgtagacc tcctgtggac tctggagaaa     720

gcccagaggt ggtgcgagcg gccagaatgg gtccacattg acaccacacc ctttgccagc     780

ctcctcaaga acctgggcac gctctttggc ctggggctgg ctctcaactc cagcatgtac     840

agggagagct gcaaggggaa actcagcaag tggctcccat tccgcctcag ctctattgta     900

gcctccctcg tcctcctgca cgtctttgac tccttgaaac ccccatccca agtcgagctg     960

gtcttctacg tcttgtcctt ctgcaagagt gcggtagtgc ccctggcatc cgtcagtgtc    1020

atcccctact gcctcgccca ggtcctgggc cagccgcaca agaagtcgtt gtaa          1074


<210> 21
<211> 1074
<212> DNA
<213> Artificial Sequence


<220> 
<223> Nucleic acid coding modified G6Pase-a (L292F)

<400> 21
atggaggaag gaatgaatgt tctccatgac tttgggatcc agtcaacaca ttacctccag      60

gtgaattacc aagactccca ggactggttc atcttggtgt ccgtgatcgc agacctcagg     120

aatgccttct acgtcctctt ccccatctgg ttccatcttc aggaagctgt gggcattaaa     180

ctcctttggg tagctgtgat tggagactgg ctcaacctcg tctttaagtg gattctcttt     240

ggacagcgtc catactggtg ggttttggat actgactact acagcaacac ttccgtgccc     300

ctgataaagc agttccctgt aacctgtgag actggaccag ggagcccctc tggccatgcc     360

atgggcacag caggtgtata ctacgtgatg gtcacatcta ctctttccat ctttcaggga     420

aagataaagc cgacctacag atttcggtgc ttgaatgtca ttttgtggtt gggattctgg     480

gctgtgcagc tgaatgtctg tctgtcacga atctaccttg ctgctcattt tcctcatcaa     540

gttgttgctg gagtcctgtc aggcattgct gttgcagaaa ctttcagcca catccacagc     600

atctataatg ccagcctcaa gaaatatttt ctcattacct tcttcctgtt cagcttcgcc     660

atcggatttt atctgctgct caagggactg ggtgtagacc tcctgtggac tctggagaaa     720

gcccagaggt ggtgcgagca gccagaatgg gtccacattg acaccacacc ctttgccagc     780

ctcctcaaga acctgggcac gctctttggc ctggggctgg ctctcaactc cagcatgtac     840

agggagagct gcaaggggaa actcagcaag tggttcccat tccgcctcag ctctattgta     900

gcctccctcg tcctcctgca cgtctttgac tccttgaaac ccccatccca agtcgagctg     960

gtcttctacg tcttgtcctt ctgcaagagt gcggtagtgc ccctggcatc cgtcagtgtc    1020

atcccctact gcctcgccca ggtcctgggc cagccgcaca agaagtcgtt gtaa          1074


<210> 22
<211> 1074
<212> DNA
<213> Artificial Sequence


<220> 
<223> Nucleic acid coding modified G6Pase-a (S298C)

<400> 22
atggaggaag gaatgaatgt tctccatgac tttgggatcc agtcaacaca ttacctccag      60

gtgaattacc aagactccca ggactggttc atcttggtgt ccgtgatcgc agacctcagg     120

aatgccttct acgtcctctt ccccatctgg ttccatcttc aggaagctgt gggcattaaa     180

ctcctttggg tagctgtgat tggagactgg ctcaacctcg tctttaagtg gattctcttt     240

ggacagcgtc catactggtg ggttttggat actgactact acagcaacac ttccgtgccc     300

ctgataaagc agttccctgt aacctgtgag actggaccag ggagcccctc tggccatgcc     360

atgggcacag caggtgtata ctacgtgatg gtcacatcta ctctttccat ctttcaggga     420

aagataaagc cgacctacag atttcggtgc ttgaatgtca ttttgtggtt gggattctgg     480

gctgtgcagc tgaatgtctg tctgtcacga atctaccttg ctgctcattt tcctcatcaa     540

gttgttgctg gagtcctgtc aggcattgct gttgcagaaa ctttcagcca catccacagc     600

atctataatg ccagcctcaa gaaatatttt ctcattacct tcttcctgtt cagcttcgcc     660

atcggatttt atctgctgct caagggactg ggtgtagacc tcctgtggac tctggagaaa     720

gcccagaggt ggtgcgagca gccagaatgg gtccacattg acaccacacc ctttgccagc     780

ctcctcaaga acctgggcac gctctttggc ctggggctgg ctctcaactc cagcatgtac     840

agggagagct gcaaggggaa actcagcaag tggctcccat tccgcctcag ctgcattgta     900

gcctccctcg tcctcctgca cgtctttgac tccttgaaac ccccatccca agtcgagctg     960

gtcttctacg tcttgtcctt ctgcaagagt gcggtagtgc ccctggcatc cgtcagtgtc    1020

atcccctact gcctcgccca ggtcctgggc cagccgcaca agaagtcgtt gtaa          1074


<210> 23
<211> 1074
<212> DNA
<213> Artificial Sequence


<220> 
<223> Nucleic acid coding modified G6Pase-a (A301V)

<400> 23
atggaggaag gaatgaatgt tctccatgac tttgggatcc agtcaacaca ttacctccag      60

gtgaattacc aagactccca ggactggttc atcttggtgt ccgtgatcgc agacctcagg     120

aatgccttct acgtcctctt ccccatctgg ttccatcttc aggaagctgt gggcattaaa     180

ctcctttggg tagctgtgat tggagactgg ctcaacctcg tctttaagtg gattctcttt     240

ggacagcgtc catactggtg ggttttggat actgactact acagcaacac ttccgtgccc     300

ctgataaagc agttccctgt aacctgtgag actggaccag ggagcccctc tggccatgcc     360

atgggcacag caggtgtata ctacgtgatg gtcacatcta ctctttccat ctttcaggga     420

aagataaagc cgacctacag atttcggtgc ttgaatgtca ttttgtggtt gggattctgg     480

gctgtgcagc tgaatgtctg tctgtcacga atctaccttg ctgctcattt tcctcatcaa     540

gttgttgctg gagtcctgtc aggcattgct gttgcagaaa ctttcagcca catccacagc     600

atctataatg ccagcctcaa gaaatatttt ctcattacct tcttcctgtt cagcttcgcc     660

atcggatttt atctgctgct caagggactg ggtgtagacc tcctgtggac tctggagaaa     720

gcccagaggt ggtgcgagca gccagaatgg gtccacattg acaccacacc ctttgccagc     780

ctcctcaaga acctgggcac gctctttggc ctggggctgg ctctcaactc cagcatgtac     840

agggagagct gcaaggggaa actcagcaag tggctcccat tccgcctcag ctctattgta     900

gtgtccctcg tcctcctgca cgtctttgac tccttgaaac ccccatccca agtcgagctg     960

gtcttctacg tcttgtcctt ctgcaagagt gcggtagtgc ccctggcatc cgtcagtgtc    1020

atcccctact gcctcgccca ggtcctgggc cagccgcaca agaagtcgtt gtaa          1074


<210> 24
<211> 1074
<212> DNA
<213> Artificial Sequence


<220> 
<223> Nucleic acid coding modified G6Pase-a (V318T)

<400> 24
atggaggaag gaatgaatgt tctccatgac tttgggatcc agtcaacaca ttacctccag      60

gtgaattacc aagactccca ggactggttc atcttggtgt ccgtgatcgc agacctcagg     120

aatgccttct acgtcctctt ccccatctgg ttccatcttc aggaagctgt gggcattaaa     180

ctcctttggg tagctgtgat tggagactgg ctcaacctcg tctttaagtg gattctcttt     240

ggacagcgtc catactggtg ggttttggat actgactact acagcaacac ttccgtgccc     300

ctgataaagc agttccctgt aacctgtgag actggaccag ggagcccctc tggccatgcc     360

atgggcacag caggtgtata ctacgtgatg gtcacatcta ctctttccat ctttcaggga     420

aagataaagc cgacctacag atttcggtgc ttgaatgtca ttttgtggtt gggattctgg     480

gctgtgcagc tgaatgtctg tctgtcacga atctaccttg ctgctcattt tcctcatcaa     540

gttgttgctg gagtcctgtc aggcattgct gttgcagaaa ctttcagcca catccacagc     600

atctataatg ccagcctcaa gaaatatttt ctcattacct tcttcctgtt cagcttcgcc     660

atcggatttt atctgctgct caagggactg ggtgtagacc tcctgtggac tctggagaaa     720

gcccagaggt ggtgcgagca gccagaatgg gtccacattg acaccacacc ctttgccagc     780

ctcctcaaga acctgggcac gctctttggc ctggggctgg ctctcaactc cagcatgtac     840

agggagagct gcaaggggaa actcagcaag tggctcccat tccgcctcag ctctattgta     900

gcctccctcg tcctcctgca cgtctttgac tccttgaaac ccccatccca aactgagctg     960

gtcttctacg tcttgtcctt ctgcaagagt gcggtagtgc ccctggcatc cgtcagtgtc    1020

atcccctact gcctcgccca ggtcctgggc cagccgcaca agaagtcgtt gtaa          1074


<210> 25
<211> 1074
<212> DNA
<213> Artificial Sequence


<220> 
<223> Nucleic acid coding modified G6Pase-a (V324T)

<400> 25
atggaggaag gaatgaatgt tctccatgac tttgggatcc agtcaacaca ttacctccag      60

gtgaattacc aagactccca ggactggttc atcttggtgt ccgtgatcgc agacctcagg     120

aatgccttct acgtcctctt ccccatctgg ttccatcttc aggaagctgt gggcattaaa     180

ctcctttggg tagctgtgat tggagactgg ctcaacctcg tctttaagtg gattctcttt     240

ggacagcgtc catactggtg ggttttggat actgactact acagcaacac ttccgtgccc     300

ctgataaagc agttccctgt aacctgtgag actggaccag ggagcccctc tggccatgcc     360

atgggcacag caggtgtata ctacgtgatg gtcacatcta ctctttccat ctttcaggga     420

aagataaagc cgacctacag atttcggtgc ttgaatgtca ttttgtggtt gggattctgg     480

gctgtgcagc tgaatgtctg tctgtcacga atctaccttg ctgctcattt tcctcatcaa     540

gttgttgctg gagtcctgtc aggcattgct gttgcagaaa ctttcagcca catccacagc     600

atctataatg ccagcctcaa gaaatatttt ctcattacct tcttcctgtt cagcttcgcc     660

atcggatttt atctgctgct caagggactg ggtgtagacc tcctgtggac tctggagaaa     720

gcccagaggt ggtgcgagca gccagaatgg gtccacattg acaccacacc ctttgccagc     780

ctcctcaaga acctgggcac gctctttggc ctggggctgg ctctcaactc cagcatgtac     840

agggagagct gcaaggggaa actcagcaag tggctcccat tccgcctcag ctctattgta     900

gcctccctcg tcctcctgca cgtctttgac tccttgaaac ccccatccca agtcgagctg     960

gtcttctaca ccttgtcctt ctgcaagagt gcggtagtgc ccctggcatc cgtcagtgtc    1020

atcccctact gcctcgccca ggtcctgggc cagccgcaca agaagtcgtt gtaa          1074


<210> 26
<211> 1074
<212> DNA
<213> Artificial Sequence


<220> 
<223> Nucleic acid coding modified G6Pase-a (V332A)

<400> 26
atggaggaag gaatgaatgt tctccatgac tttgggatcc agtcaacaca ttacctccag      60

gtgaattacc aagactccca ggactggttc atcttggtgt ccgtgatcgc agacctcagg     120

aatgccttct acgtcctctt ccccatctgg ttccatcttc aggaagctgt gggcattaaa     180

ctcctttggg tagctgtgat tggagactgg ctcaacctcg tctttaagtg gattctcttt     240

ggacagcgtc catactggtg ggttttggat actgactact acagcaacac ttccgtgccc     300

ctgataaagc agttccctgt aacctgtgag actggaccag ggagcccctc tggccatgcc     360

atgggcacag caggtgtata ctacgtgatg gtcacatcta ctctttccat ctttcaggga     420

aagataaagc cgacctacag atttcggtgc ttgaatgtca ttttgtggtt gggattctgg     480

gctgtgcagc tgaatgtctg tctgtcacga atctaccttg ctgctcattt tcctcatcaa     540

gttgttgctg gagtcctgtc aggcattgct gttgcagaaa ctttcagcca catccacagc     600

atctataatg ccagcctcaa gaaatatttt ctcattacct tcttcctgtt cagcttcgcc     660

atcggatttt atctgctgct caagggactg ggtgtagacc tcctgtggac tctggagaaa     720

gcccagaggt ggtgcgagca gccagaatgg gtccacattg acaccacacc ctttgccagc     780

ctcctcaaga acctgggcac gctctttggc ctggggctgg ctctcaactc cagcatgtac     840

agggagagct gcaaggggaa actcagcaag tggctcccat tccgcctcag ctctattgta     900

gcctccctcg tcctcctgca cgtctttgac tccttgaaac ccccatccca agtcgagctg     960

gtcttctacg tcttgtcctt ctgcaagagt gcggcagtgc ccctggcatc cgtcagtgtc    1020

atcccctact gcctcgccca ggtcctgggc cagccgcaca agaagtcgtt gtaa          1074


<210> 27
<211> 1074
<212> DNA
<213> Artificial Sequence


<220> 
<223> Nucleic acid coding modified G6Pase-a (Q347R)

<400> 27
atggaggaag gaatgaatgt tctccatgac tttgggatcc agtcaacaca ttacctccag      60

gtgaattacc aagactccca ggactggttc atcttggtgt ccgtgatcgc agacctcagg     120

aatgccttct acgtcctctt ccccatctgg ttccatcttc aggaagctgt gggcattaaa     180

ctcctttggg tagctgtgat tggagactgg ctcaacctcg tctttaagtg gattctcttt     240

ggacagcgtc catactggtg ggttttggat actgactact acagcaacac ttccgtgccc     300

ctgataaagc agttccctgt aacctgtgag actggaccag ggagcccctc tggccatgcc     360

atgggcacag caggtgtata ctacgtgatg gtcacatcta ctctttccat ctttcaggga     420

aagataaagc cgacctacag atttcggtgc ttgaatgtca ttttgtggtt gggattctgg     480

gctgtgcagc tgaatgtctg tctgtcacga atctaccttg ctgctcattt tcctcatcaa     540

gttgttgctg gagtcctgtc aggcattgct gttgcagaaa ctttcagcca catccacagc     600

atctataatg ccagcctcaa gaaatatttt ctcattacct tcttcctgtt cagcttcgcc     660

atcggatttt atctgctgct caagggactg ggtgtagacc tcctgtggac tctggagaaa     720

gcccagaggt ggtgcgagca gccagaatgg gtccacattg acaccacacc ctttgccagc     780

ctcctcaaga acctgggcac gctctttggc ctggggctgg ctctcaactc cagcatgtac     840

agggagagct gcaaggggaa actcagcaag tggctcccat tccgcctcag ctctattgta     900

gcctccctcg tcctcctgca cgtctttgac tccttgaaac ccccatccca agtcgagctg     960

gtcttctacg tcttgtcctt ctgcaagagt gcggtagtgc ccctggcatc cgtcagtgtc    1020

atcccctact gcctcgcccg ggtcctgggc cagccgcaca agaagtcgtt gtaa          1074


<210> 28
<211> 1074
<212> DNA
<213> Artificial Sequence


<220> 
<223> Nucleic acid coding modified G6Pase-a (L349F)

<400> 28
atggaggaag gaatgaatgt tctccatgac tttgggatcc agtcaacaca ttacctccag      60

gtgaattacc aagactccca ggactggttc atcttggtgt ccgtgatcgc agacctcagg     120

aatgccttct acgtcctctt ccccatctgg ttccatcttc aggaagctgt gggcattaaa     180

ctcctttggg tagctgtgat tggagactgg ctcaacctcg tctttaagtg gattctcttt     240

ggacagcgtc catactggtg ggttttggat actgactact acagcaacac ttccgtgccc     300

ctgataaagc agttccctgt aacctgtgag actggaccag ggagcccctc tggccatgcc     360

atgggcacag caggtgtata ctacgtgatg gtcacatcta ctctttccat ctttcaggga     420

aagataaagc cgacctacag atttcggtgc ttgaatgtca ttttgtggtt gggattctgg     480

gctgtgcagc tgaatgtctg tctgtcacga atctaccttg ctgctcattt tcctcatcaa     540

gttgttgctg gagtcctgtc aggcattgct gttgcagaaa ctttcagcca catccacagc     600

atctataatg ccagcctcaa gaaatatttt ctcattacct tcttcctgtt cagcttcgcc     660

atcggatttt atctgctgct caagggactg ggtgtagacc tcctgtggac tctggagaaa     720

gcccagaggt ggtgcgagca gccagaatgg gtccacattg acaccacacc ctttgccagc     780

ctcctcaaga acctgggcac gctctttggc ctggggctgg ctctcaactc cagcatgtac     840

agggagagct gcaaggggaa actcagcaag tggctcccat tccgcctcag ctctattgta     900

gcctccctcg tcctcctgca cgtctttgac tccttgaaac ccccatccca agtcgagctg     960

gtcttctacg tcttgtcctt ctgcaagagt gcggtagtgc ccctggcatc cgtcagtgtc    1020

atcccctact gcctcgccca ggtcttcggc cagccgcaca agaagtcgtt gtaa          1074


<210> 29
<211> 357
<212> PRT
<213> Artificial Sequence


<220> 
<223> Modified G6Pase-a (R3K)

<400> 29
Met Glu Lys Gly Met Asn Val Leu His Asp Phe Gly Ile Gln Ser Thr 
1               5                   10                  15      
His Tyr Leu Gln Val Asn Tyr Gln Asp Ser Gln Asp Trp Phe Ile Leu 
            20                  25                  30          
Val Ser Val Ile Ala Asp Leu Arg Asn Ala Phe Tyr Val Leu Phe Pro 
        35                  40                  45              
Ile Trp Phe His Leu Gln Glu Ala Val Gly Ile Lys Leu Leu Trp Val 
    50                  55                  60                  
Ala Val Ile Gly Asp Trp Leu Asn Leu Val Phe Lys Trp Ile Leu Phe 
65                  70                  75                  80  
Gly Gln Arg Pro Tyr Trp Trp Val Leu Asp Thr Asp Tyr Tyr Ser Asn 
                85                  90                  95      
Thr Ser Val Pro Leu Ile Lys Gln Phe Pro Val Thr Cys Glu Thr Gly 
            100                 105                 110         
Pro Gly Ser Pro Ser Gly His Ala Met Gly Thr Ala Gly Val Tyr Tyr 
        115                 120                 125             
Val Met Val Thr Ser Thr Leu Ser Ile Phe Gln Gly Lys Ile Lys Pro 
    130                 135                 140                 
Thr Tyr Arg Phe Arg Cys Leu Asn Val Ile Leu Trp Leu Gly Phe Trp 
145                 150                 155                 160 
Ala Val Gln Leu Asn Val Cys Leu Ser Arg Ile Tyr Leu Ala Ala His 
                165                 170                 175     
Phe Pro His Gln Val Val Ala Gly Val Leu Ser Gly Ile Ala Val Ala 
            180                 185                 190         
Glu Thr Phe Ser His Ile His Ser Ile Tyr Asn Ala Ser Leu Lys Lys 
        195                 200                 205             
Tyr Phe Leu Ile Thr Phe Phe Leu Phe Ser Phe Ala Ile Gly Phe Tyr 
    210                 215                 220                 
Leu Leu Leu Lys Gly Leu Gly Val Asp Leu Leu Trp Thr Leu Glu Lys 
225                 230                 235                 240 
Ala Gln Arg Trp Cys Glu Gln Pro Glu Trp Val His Ile Asp Thr Thr 
                245                 250                 255     
Pro Phe Ala Ser Leu Leu Lys Asn Leu Gly Thr Leu Phe Gly Leu Gly 
            260                 265                 270         
Leu Ala Leu Asn Ser Ser Met Tyr Arg Glu Ser Cys Lys Gly Lys Leu 
        275                 280                 285             
Ser Lys Trp Leu Pro Phe Arg Leu Ser Ser Ile Val Ala Ser Leu Val 
    290                 295                 300                 
Leu Leu His Val Phe Asp Ser Leu Lys Pro Pro Ser Gln Val Glu Leu 
305                 310                 315                 320 
Val Phe Tyr Val Leu Ser Phe Cys Lys Ser Ala Val Val Pro Leu Ala 
                325                 330                 335     
Ser Val Ser Val Ile Pro Tyr Cys Leu Ala Gln Val Leu Gly Gln Pro 
            340                 345                 350         
His Lys Lys Ser Leu 
        355         

<210> 30
<211> 357
<212> PRT
<213> Artificial Sequence


<220> 
<223> Modified G6Pase-a (Q54R)

<400> 30
Met Glu Glu Gly Met Asn Val Leu His Asp Phe Gly Ile Gln Ser Thr 
1               5                   10                  15      
His Tyr Leu Gln Val Asn Tyr Gln Asp Ser Gln Asp Trp Phe Ile Leu 
            20                  25                  30          
Val Ser Val Ile Ala Asp Leu Arg Asn Ala Phe Tyr Val Leu Phe Pro 
        35                  40                  45              
Ile Trp Phe His Leu Arg Glu Ala Val Gly Ile Lys Leu Leu Trp Val 
    50                  55                  60                  
Ala Val Ile Gly Asp Trp Leu Asn Leu Val Phe Lys Trp Ile Leu Phe 
65                  70                  75                  80  
Gly Gln Arg Pro Tyr Trp Trp Val Leu Asp Thr Asp Tyr Tyr Ser Asn 
                85                  90                  95      
Thr Ser Val Pro Leu Ile Lys Gln Phe Pro Val Thr Cys Glu Thr Gly 
            100                 105                 110         
Pro Gly Ser Pro Ser Gly His Ala Met Gly Thr Ala Gly Val Tyr Tyr 
        115                 120                 125             
Val Met Val Thr Ser Thr Leu Ser Ile Phe Gln Gly Lys Ile Lys Pro 
    130                 135                 140                 
Thr Tyr Arg Phe Arg Cys Leu Asn Val Ile Leu Trp Leu Gly Phe Trp 
145                 150                 155                 160 
Ala Val Gln Leu Asn Val Cys Leu Ser Arg Ile Tyr Leu Ala Ala His 
                165                 170                 175     
Phe Pro His Gln Val Val Ala Gly Val Leu Ser Gly Ile Ala Val Ala 
            180                 185                 190         
Glu Thr Phe Ser His Ile His Ser Ile Tyr Asn Ala Ser Leu Lys Lys 
        195                 200                 205             
Tyr Phe Leu Ile Thr Phe Phe Leu Phe Ser Phe Ala Ile Gly Phe Tyr 
    210                 215                 220                 
Leu Leu Leu Lys Gly Leu Gly Val Asp Leu Leu Trp Thr Leu Glu Lys 
225                 230                 235                 240 
Ala Gln Arg Trp Cys Glu Gln Pro Glu Trp Val His Ile Asp Thr Thr 
                245                 250                 255     
Pro Phe Ala Ser Leu Leu Lys Asn Leu Gly Thr Leu Phe Gly Leu Gly 
            260                 265                 270         
Leu Ala Leu Asn Ser Ser Met Tyr Arg Glu Ser Cys Lys Gly Lys Leu 
        275                 280                 285             
Ser Lys Trp Leu Pro Phe Arg Leu Ser Ser Ile Val Ala Ser Leu Val 
    290                 295                 300                 
Leu Leu His Val Phe Asp Ser Leu Lys Pro Pro Ser Gln Val Glu Leu 
305                 310                 315                 320 
Val Phe Tyr Val Leu Ser Phe Cys Lys Ser Ala Val Val Pro Leu Ala 
                325                 330                 335     
Ser Val Ser Val Ile Pro Tyr Cys Leu Ala Gln Val Leu Gly Gln Pro 
            340                 345                 350         
His Lys Lys Ser Leu 
        355         

<210> 31
<211> 357
<212> PRT
<213> Artificial Sequence


<220> 
<223> Modified G6Pase-a (Q139R)

<400> 31
Met Glu Glu Gly Met Asn Val Leu His Asp Phe Gly Ile Gln Ser Thr 
1               5                   10                  15      
His Tyr Leu Gln Val Asn Tyr Gln Asp Ser Gln Asp Trp Phe Ile Leu 
            20                  25                  30          
Val Ser Val Ile Ala Asp Leu Arg Asn Ala Phe Tyr Val Leu Phe Pro 
        35                  40                  45              
Ile Trp Phe His Leu Gln Glu Ala Val Gly Ile Lys Leu Leu Trp Val 
    50                  55                  60                  
Ala Val Ile Gly Asp Trp Leu Asn Leu Val Phe Lys Trp Ile Leu Phe 
65                  70                  75                  80  
Gly Gln Arg Pro Tyr Trp Trp Val Leu Asp Thr Asp Tyr Tyr Ser Asn 
                85                  90                  95      
Thr Ser Val Pro Leu Ile Lys Gln Phe Pro Val Thr Cys Glu Thr Gly 
            100                 105                 110         
Pro Gly Ser Pro Ser Gly His Ala Met Gly Thr Ala Gly Val Tyr Tyr 
        115                 120                 125             
Val Met Val Thr Ser Thr Leu Ser Ile Phe Arg Gly Lys Ile Lys Pro 
    130                 135                 140                 
Thr Tyr Arg Phe Arg Cys Leu Asn Val Ile Leu Trp Leu Gly Phe Trp 
145                 150                 155                 160 
Ala Val Gln Leu Asn Val Cys Leu Ser Arg Ile Tyr Leu Ala Ala His 
                165                 170                 175     
Phe Pro His Gln Val Val Ala Gly Val Leu Ser Gly Ile Ala Val Ala 
            180                 185                 190         
Glu Thr Phe Ser His Ile His Ser Ile Tyr Asn Ala Ser Leu Lys Lys 
        195                 200                 205             
Tyr Phe Leu Ile Thr Phe Phe Leu Phe Ser Phe Ala Ile Gly Phe Tyr 
    210                 215                 220                 
Leu Leu Leu Lys Gly Leu Gly Val Asp Leu Leu Trp Thr Leu Glu Lys 
225                 230                 235                 240 
Ala Gln Arg Trp Cys Glu Gln Pro Glu Trp Val His Ile Asp Thr Thr 
                245                 250                 255     
Pro Phe Ala Ser Leu Leu Lys Asn Leu Gly Thr Leu Phe Gly Leu Gly 
            260                 265                 270         
Leu Ala Leu Asn Ser Ser Met Tyr Arg Glu Ser Cys Lys Gly Lys Leu 
        275                 280                 285             
Ser Lys Trp Leu Pro Phe Arg Leu Ser Ser Ile Val Ala Ser Leu Val 
    290                 295                 300                 
Leu Leu His Val Phe Asp Ser Leu Lys Pro Pro Ser Gln Val Glu Leu 
305                 310                 315                 320 
Val Phe Tyr Val Leu Ser Phe Cys Lys Ser Ala Val Val Pro Leu Ala 
                325                 330                 335     
Ser Val Ser Val Ile Pro Tyr Cys Leu Ala Gln Val Leu Gly Gln Pro 
            340                 345                 350         
His Lys Lys Ser Leu 
        355         

<210> 32
<211> 357
<212> PRT
<213> Artificial Sequence


<220> 
<223> Modified G6Pase-a (I142K)

<400> 32
Met Glu Glu Gly Met Asn Val Leu His Asp Phe Gly Ile Gln Ser Thr 
1               5                   10                  15      
His Tyr Leu Gln Val Asn Tyr Gln Asp Ser Gln Asp Trp Phe Ile Leu 
            20                  25                  30          
Val Ser Val Ile Ala Asp Leu Arg Asn Ala Phe Tyr Val Leu Phe Pro 
        35                  40                  45              
Ile Trp Phe His Leu Gln Glu Ala Val Gly Ile Lys Leu Leu Trp Val 
    50                  55                  60                  
Ala Val Ile Gly Asp Trp Leu Asn Leu Val Phe Lys Trp Ile Leu Phe 
65                  70                  75                  80  
Gly Gln Arg Pro Tyr Trp Trp Val Leu Asp Thr Asp Tyr Tyr Ser Asn 
                85                  90                  95      
Thr Ser Val Pro Leu Ile Lys Gln Phe Pro Val Thr Cys Glu Thr Gly 
            100                 105                 110         
Pro Gly Ser Pro Ser Gly His Ala Met Gly Thr Ala Gly Val Tyr Tyr 
        115                 120                 125             
Val Met Val Thr Ser Thr Leu Ser Ile Phe Gln Gly Lys Lys Lys Pro 
    130                 135                 140                 
Thr Tyr Arg Phe Arg Cys Leu Asn Val Ile Leu Trp Leu Gly Phe Trp 
145                 150                 155                 160 
Ala Val Gln Leu Asn Val Cys Leu Ser Arg Ile Tyr Leu Ala Ala His 
                165                 170                 175     
Phe Pro His Gln Val Val Ala Gly Val Leu Ser Gly Ile Ala Val Ala 
            180                 185                 190         
Glu Thr Phe Ser His Ile His Ser Ile Tyr Asn Ala Ser Leu Lys Lys 
        195                 200                 205             
Tyr Phe Leu Ile Thr Phe Phe Leu Phe Ser Phe Ala Ile Gly Phe Tyr 
    210                 215                 220                 
Leu Leu Leu Lys Gly Leu Gly Val Asp Leu Leu Trp Thr Leu Glu Lys 
225                 230                 235                 240 
Ala Gln Arg Trp Cys Glu Gln Pro Glu Trp Val His Ile Asp Thr Thr 
                245                 250                 255     
Pro Phe Ala Ser Leu Leu Lys Asn Leu Gly Thr Leu Phe Gly Leu Gly 
            260                 265                 270         
Leu Ala Leu Asn Ser Ser Met Tyr Arg Glu Ser Cys Lys Gly Lys Leu 
        275                 280                 285             
Ser Lys Trp Leu Pro Phe Arg Leu Ser Ser Ile Val Ala Ser Leu Val 
    290                 295                 300                 
Leu Leu His Val Phe Asp Ser Leu Lys Pro Pro Ser Gln Val Glu Leu 
305                 310                 315                 320 
Val Phe Tyr Val Leu Ser Phe Cys Lys Ser Ala Val Val Pro Leu Ala 
                325                 330                 335     
Ser Val Ser Val Ile Pro Tyr Cys Leu Ala Gln Val Leu Gly Gln Pro 
            340                 345                 350         
His Lys Lys Ser Leu 
        355         

<210> 33
<211> 357
<212> PRT
<213> Artificial Sequence


<220> 
<223> Modified G6Pase-a (S196R)

<400> 33
Met Glu Glu Gly Met Asn Val Leu His Asp Phe Gly Ile Gln Ser Thr 
1               5                   10                  15      
His Tyr Leu Gln Val Asn Tyr Gln Asp Ser Gln Asp Trp Phe Ile Leu 
            20                  25                  30          
Val Ser Val Ile Ala Asp Leu Arg Asn Ala Phe Tyr Val Leu Phe Pro 
        35                  40                  45              
Ile Trp Phe His Leu Gln Glu Ala Val Gly Ile Lys Leu Leu Trp Val 
    50                  55                  60                  
Ala Val Ile Gly Asp Trp Leu Asn Leu Val Phe Lys Trp Ile Leu Phe 
65                  70                  75                  80  
Gly Gln Arg Pro Tyr Trp Trp Val Leu Asp Thr Asp Tyr Tyr Ser Asn 
                85                  90                  95      
Thr Ser Val Pro Leu Ile Lys Gln Phe Pro Val Thr Cys Glu Thr Gly 
            100                 105                 110         
Pro Gly Ser Pro Ser Gly His Ala Met Gly Thr Ala Gly Val Tyr Tyr 
        115                 120                 125             
Val Met Val Thr Ser Thr Leu Ser Ile Phe Gln Gly Lys Ile Lys Pro 
    130                 135                 140                 
Thr Tyr Arg Phe Arg Cys Leu Asn Val Ile Leu Trp Leu Gly Phe Trp 
145                 150                 155                 160 
Ala Val Gln Leu Asn Val Cys Leu Ser Arg Ile Tyr Leu Ala Ala His 
                165                 170                 175     
Phe Pro His Gln Val Val Ala Gly Val Leu Ser Gly Ile Ala Val Ala 
            180                 185                 190         
Glu Thr Phe Arg His Ile His Ser Ile Tyr Asn Ala Ser Leu Lys Lys 
        195                 200                 205             
Tyr Phe Leu Ile Thr Phe Phe Leu Phe Ser Phe Ala Ile Gly Phe Tyr 
    210                 215                 220                 
Leu Leu Leu Lys Gly Leu Gly Val Asp Leu Leu Trp Thr Leu Glu Lys 
225                 230                 235                 240 
Ala Gln Arg Trp Cys Glu Gln Pro Glu Trp Val His Ile Asp Thr Thr 
                245                 250                 255     
Pro Phe Ala Ser Leu Leu Lys Asn Leu Gly Thr Leu Phe Gly Leu Gly 
            260                 265                 270         
Leu Ala Leu Asn Ser Ser Met Tyr Arg Glu Ser Cys Lys Gly Lys Leu 
        275                 280                 285             
Ser Lys Trp Leu Pro Phe Arg Leu Ser Ser Ile Val Ala Ser Leu Val 
    290                 295                 300                 
Leu Leu His Val Phe Asp Ser Leu Lys Pro Pro Ser Gln Val Glu Leu 
305                 310                 315                 320 
Val Phe Tyr Val Leu Ser Phe Cys Lys Ser Ala Val Val Pro Leu Ala 
                325                 330                 335     
Ser Val Ser Val Ile Pro Tyr Cys Leu Ala Gln Val Leu Gly Gln Pro 
            340                 345                 350         
His Lys Lys Ser Leu 
        355         

<210> 34
<211> 357
<212> PRT
<213> Artificial Sequence


<220> 
<223> Modified G6Pase-a (H199Q)

<400> 34
Met Glu Glu Gly Met Asn Val Leu His Asp Phe Gly Ile Gln Ser Thr 
1               5                   10                  15      
His Tyr Leu Gln Val Asn Tyr Gln Asp Ser Gln Asp Trp Phe Ile Leu 
            20                  25                  30          
Val Ser Val Ile Ala Asp Leu Arg Asn Ala Phe Tyr Val Leu Phe Pro 
        35                  40                  45              
Ile Trp Phe His Leu Gln Glu Ala Val Gly Ile Lys Leu Leu Trp Val 
    50                  55                  60                  
Ala Val Ile Gly Asp Trp Leu Asn Leu Val Phe Lys Trp Ile Leu Phe 
65                  70                  75                  80  
Gly Gln Arg Pro Tyr Trp Trp Val Leu Asp Thr Asp Tyr Tyr Ser Asn 
                85                  90                  95      
Thr Ser Val Pro Leu Ile Lys Gln Phe Pro Val Thr Cys Glu Thr Gly 
            100                 105                 110         
Pro Gly Ser Pro Ser Gly His Ala Met Gly Thr Ala Gly Val Tyr Tyr 
        115                 120                 125             
Val Met Val Thr Ser Thr Leu Ser Ile Phe Gln Gly Lys Ile Lys Pro 
    130                 135                 140                 
Thr Tyr Arg Phe Arg Cys Leu Asn Val Ile Leu Trp Leu Gly Phe Trp 
145                 150                 155                 160 
Ala Val Gln Leu Asn Val Cys Leu Ser Arg Ile Tyr Leu Ala Ala His 
                165                 170                 175     
Phe Pro His Gln Val Val Ala Gly Val Leu Ser Gly Ile Ala Val Ala 
            180                 185                 190         
Glu Thr Phe Ser His Ile Gln Ser Ile Tyr Asn Ala Ser Leu Lys Lys 
        195                 200                 205             
Tyr Phe Leu Ile Thr Phe Phe Leu Phe Ser Phe Ala Ile Gly Phe Tyr 
    210                 215                 220                 
Leu Leu Leu Lys Gly Leu Gly Val Asp Leu Leu Trp Thr Leu Glu Lys 
225                 230                 235                 240 
Ala Gln Arg Trp Cys Glu Gln Pro Glu Trp Val His Ile Asp Thr Thr 
                245                 250                 255     
Pro Phe Ala Ser Leu Leu Lys Asn Leu Gly Thr Leu Phe Gly Leu Gly 
            260                 265                 270         
Leu Ala Leu Asn Ser Ser Met Tyr Arg Glu Ser Cys Lys Gly Lys Leu 
        275                 280                 285             
Ser Lys Trp Leu Pro Phe Arg Leu Ser Ser Ile Val Ala Ser Leu Val 
    290                 295                 300                 
Leu Leu His Val Phe Asp Ser Leu Lys Pro Pro Ser Gln Val Glu Leu 
305                 310                 315                 320 
Val Phe Tyr Val Leu Ser Phe Cys Lys Ser Ala Val Val Pro Leu Ala 
                325                 330                 335     
Ser Val Ser Val Ile Pro Tyr Cys Leu Ala Gln Val Leu Gly Gln Pro 
            340                 345                 350         
His Lys Lys Ser Leu 
        355         

<210> 35
<211> 357
<212> PRT
<213> Artificial Sequence


<220> 
<223> Modified G6Pase-a (Q242R)

<400> 35
Met Glu Glu Gly Met Asn Val Leu His Asp Phe Gly Ile Gln Ser Thr 
1               5                   10                  15      
His Tyr Leu Gln Val Asn Tyr Gln Asp Ser Gln Asp Trp Phe Ile Leu 
            20                  25                  30          
Val Ser Val Ile Ala Asp Leu Arg Asn Ala Phe Tyr Val Leu Phe Pro 
        35                  40                  45              
Ile Trp Phe His Leu Gln Glu Ala Val Gly Ile Lys Leu Leu Trp Val 
    50                  55                  60                  
Ala Val Ile Gly Asp Trp Leu Asn Leu Val Phe Lys Trp Ile Leu Phe 
65                  70                  75                  80  
Gly Gln Arg Pro Tyr Trp Trp Val Leu Asp Thr Asp Tyr Tyr Ser Asn 
                85                  90                  95      
Thr Ser Val Pro Leu Ile Lys Gln Phe Pro Val Thr Cys Glu Thr Gly 
            100                 105                 110         
Pro Gly Ser Pro Ser Gly His Ala Met Gly Thr Ala Gly Val Tyr Tyr 
        115                 120                 125             
Val Met Val Thr Ser Thr Leu Ser Ile Phe Gln Gly Lys Ile Lys Pro 
    130                 135                 140                 
Thr Tyr Arg Phe Arg Cys Leu Asn Val Ile Leu Trp Leu Gly Phe Trp 
145                 150                 155                 160 
Ala Val Gln Leu Asn Val Cys Leu Ser Arg Ile Tyr Leu Ala Ala His 
                165                 170                 175     
Phe Pro His Gln Val Val Ala Gly Val Leu Ser Gly Ile Ala Val Ala 
            180                 185                 190         
Glu Thr Phe Ser His Ile His Ser Ile Tyr Asn Ala Ser Leu Lys Lys 
        195                 200                 205             
Tyr Phe Leu Ile Thr Phe Phe Leu Phe Ser Phe Ala Ile Gly Phe Tyr 
    210                 215                 220                 
Leu Leu Leu Lys Gly Leu Gly Val Asp Leu Leu Trp Thr Leu Glu Lys 
225                 230                 235                 240 
Ala Arg Arg Trp Cys Glu Gln Pro Glu Trp Val His Ile Asp Thr Thr 
                245                 250                 255     
Pro Phe Ala Ser Leu Leu Lys Asn Leu Gly Thr Leu Phe Gly Leu Gly 
            260                 265                 270         
Leu Ala Leu Asn Ser Ser Met Tyr Arg Glu Ser Cys Lys Gly Lys Leu 
        275                 280                 285             
Ser Lys Trp Leu Pro Phe Arg Leu Ser Ser Ile Val Ala Ser Leu Val 
    290                 295                 300                 
Leu Leu His Val Phe Asp Ser Leu Lys Pro Pro Ser Gln Val Glu Leu 
305                 310                 315                 320 
Val Phe Tyr Val Leu Ser Phe Cys Lys Ser Ala Val Val Pro Leu Ala 
                325                 330                 335     
Ser Val Ser Val Ile Pro Tyr Cys Leu Ala Gln Val Leu Gly Gln Pro 
            340                 345                 350         
His Lys Lys Ser Leu 
        355         

<210> 36
<211> 357
<212> PRT
<213> Artificial Sequence


<220> 
<223> Modified G6Pase-a (Q247R)

<400> 36
Met Glu Glu Gly Met Asn Val Leu His Asp Phe Gly Ile Gln Ser Thr 
1               5                   10                  15      
His Tyr Leu Gln Val Asn Tyr Gln Asp Ser Gln Asp Trp Phe Ile Leu 
            20                  25                  30          
Val Ser Val Ile Ala Asp Leu Arg Asn Ala Phe Tyr Val Leu Phe Pro 
        35                  40                  45              
Ile Trp Phe His Leu Gln Glu Ala Val Gly Ile Lys Leu Leu Trp Val 
    50                  55                  60                  
Ala Val Ile Gly Asp Trp Leu Asn Leu Val Phe Lys Trp Ile Leu Phe 
65                  70                  75                  80  
Gly Gln Arg Pro Tyr Trp Trp Val Leu Asp Thr Asp Tyr Tyr Ser Asn 
                85                  90                  95      
Thr Ser Val Pro Leu Ile Lys Gln Phe Pro Val Thr Cys Glu Thr Gly 
            100                 105                 110         
Pro Gly Ser Pro Ser Gly His Ala Met Gly Thr Ala Gly Val Tyr Tyr 
        115                 120                 125             
Val Met Val Thr Ser Thr Leu Ser Ile Phe Gln Gly Lys Ile Lys Pro 
    130                 135                 140                 
Thr Tyr Arg Phe Arg Cys Leu Asn Val Ile Leu Trp Leu Gly Phe Trp 
145                 150                 155                 160 
Ala Val Gln Leu Asn Val Cys Leu Ser Arg Ile Tyr Leu Ala Ala His 
                165                 170                 175     
Phe Pro His Gln Val Val Ala Gly Val Leu Ser Gly Ile Ala Val Ala 
            180                 185                 190         
Glu Thr Phe Ser His Ile His Ser Ile Tyr Asn Ala Ser Leu Lys Lys 
        195                 200                 205             
Tyr Phe Leu Ile Thr Phe Phe Leu Phe Ser Phe Ala Ile Gly Phe Tyr 
    210                 215                 220                 
Leu Leu Leu Lys Gly Leu Gly Val Asp Leu Leu Trp Thr Leu Glu Lys 
225                 230                 235                 240 
Ala Gln Arg Trp Cys Glu Arg Pro Glu Trp Val His Ile Asp Thr Thr 
                245                 250                 255     
Pro Phe Ala Ser Leu Leu Lys Asn Leu Gly Thr Leu Phe Gly Leu Gly 
            260                 265                 270         
Leu Ala Leu Asn Ser Ser Met Tyr Arg Glu Ser Cys Lys Gly Lys Leu 
        275                 280                 285             
Ser Lys Trp Leu Pro Phe Arg Leu Ser Ser Ile Val Ala Ser Leu Val 
    290                 295                 300                 
Leu Leu His Val Phe Asp Ser Leu Lys Pro Pro Ser Gln Val Glu Leu 
305                 310                 315                 320 
Val Phe Tyr Val Leu Ser Phe Cys Lys Ser Ala Val Val Pro Leu Ala 
                325                 330                 335     
Ser Val Ser Val Ile Pro Tyr Cys Leu Ala Gln Val Leu Gly Gln Pro 
            340                 345                 350         
His Lys Lys Ser Leu 
        355         

<210> 37
<211> 357
<212> PRT
<213> Artificial Sequence


<220> 
<223> Modified G6Pase-a (L292F)

<400> 37
Met Glu Glu Gly Met Asn Val Leu His Asp Phe Gly Ile Gln Ser Thr 
1               5                   10                  15      
His Tyr Leu Gln Val Asn Tyr Gln Asp Ser Gln Asp Trp Phe Ile Leu 
            20                  25                  30          
Val Ser Val Ile Ala Asp Leu Arg Asn Ala Phe Tyr Val Leu Phe Pro 
        35                  40                  45              
Ile Trp Phe His Leu Gln Glu Ala Val Gly Ile Lys Leu Leu Trp Val 
    50                  55                  60                  
Ala Val Ile Gly Asp Trp Leu Asn Leu Val Phe Lys Trp Ile Leu Phe 
65                  70                  75                  80  
Gly Gln Arg Pro Tyr Trp Trp Val Leu Asp Thr Asp Tyr Tyr Ser Asn 
                85                  90                  95      
Thr Ser Val Pro Leu Ile Lys Gln Phe Pro Val Thr Cys Glu Thr Gly 
            100                 105                 110         
Pro Gly Ser Pro Ser Gly His Ala Met Gly Thr Ala Gly Val Tyr Tyr 
        115                 120                 125             
Val Met Val Thr Ser Thr Leu Ser Ile Phe Gln Gly Lys Ile Lys Pro 
    130                 135                 140                 
Thr Tyr Arg Phe Arg Cys Leu Asn Val Ile Leu Trp Leu Gly Phe Trp 
145                 150                 155                 160 
Ala Val Gln Leu Asn Val Cys Leu Ser Arg Ile Tyr Leu Ala Ala His 
                165                 170                 175     
Phe Pro His Gln Val Val Ala Gly Val Leu Ser Gly Ile Ala Val Ala 
            180                 185                 190         
Glu Thr Phe Ser His Ile His Ser Ile Tyr Asn Ala Ser Leu Lys Lys 
        195                 200                 205             
Tyr Phe Leu Ile Thr Phe Phe Leu Phe Ser Phe Ala Ile Gly Phe Tyr 
    210                 215                 220                 
Leu Leu Leu Lys Gly Leu Gly Val Asp Leu Leu Trp Thr Leu Glu Lys 
225                 230                 235                 240 
Ala Gln Arg Trp Cys Glu Gln Pro Glu Trp Val His Ile Asp Thr Thr 
                245                 250                 255     
Pro Phe Ala Ser Leu Leu Lys Asn Leu Gly Thr Leu Phe Gly Leu Gly 
            260                 265                 270         
Leu Ala Leu Asn Ser Ser Met Tyr Arg Glu Ser Cys Lys Gly Lys Leu 
        275                 280                 285             
Ser Lys Trp Phe Pro Phe Arg Leu Ser Ser Ile Val Ala Ser Leu Val 
    290                 295                 300                 
Leu Leu His Val Phe Asp Ser Leu Lys Pro Pro Ser Gln Val Glu Leu 
305                 310                 315                 320 
Val Phe Tyr Val Leu Ser Phe Cys Lys Ser Ala Val Val Pro Leu Ala 
                325                 330                 335     
Ser Val Ser Val Ile Pro Tyr Cys Leu Ala Gln Val Leu Gly Gln Pro 
            340                 345                 350         
His Lys Lys Ser Leu 
        355         

<210> 38
<211> 357
<212> PRT
<213> Artificial Sequence


<220> 
<223> Modified G6Pase-a (S298C)

<400> 38
Met Glu Glu Gly Met Asn Val Leu His Asp Phe Gly Ile Gln Ser Thr 
1               5                   10                  15      
His Tyr Leu Gln Val Asn Tyr Gln Asp Ser Gln Asp Trp Phe Ile Leu 
            20                  25                  30          
Val Ser Val Ile Ala Asp Leu Arg Asn Ala Phe Tyr Val Leu Phe Pro 
        35                  40                  45              
Ile Trp Phe His Leu Gln Glu Ala Val Gly Ile Lys Leu Leu Trp Val 
    50                  55                  60                  
Ala Val Ile Gly Asp Trp Leu Asn Leu Val Phe Lys Trp Ile Leu Phe 
65                  70                  75                  80  
Gly Gln Arg Pro Tyr Trp Trp Val Leu Asp Thr Asp Tyr Tyr Ser Asn 
                85                  90                  95      
Thr Ser Val Pro Leu Ile Lys Gln Phe Pro Val Thr Cys Glu Thr Gly 
            100                 105                 110         
Pro Gly Ser Pro Ser Gly His Ala Met Gly Thr Ala Gly Val Tyr Tyr 
        115                 120                 125             
Val Met Val Thr Ser Thr Leu Ser Ile Phe Gln Gly Lys Ile Lys Pro 
    130                 135                 140                 
Thr Tyr Arg Phe Arg Cys Leu Asn Val Ile Leu Trp Leu Gly Phe Trp 
145                 150                 155                 160 
Ala Val Gln Leu Asn Val Cys Leu Ser Arg Ile Tyr Leu Ala Ala His 
                165                 170                 175     
Phe Pro His Gln Val Val Ala Gly Val Leu Ser Gly Ile Ala Val Ala 
            180                 185                 190         
Glu Thr Phe Ser His Ile His Ser Ile Tyr Asn Ala Ser Leu Lys Lys 
        195                 200                 205             
Tyr Phe Leu Ile Thr Phe Phe Leu Phe Ser Phe Ala Ile Gly Phe Tyr 
    210                 215                 220                 
Leu Leu Leu Lys Gly Leu Gly Val Asp Leu Leu Trp Thr Leu Glu Lys 
225                 230                 235                 240 
Ala Gln Arg Trp Cys Glu Gln Pro Glu Trp Val His Ile Asp Thr Thr 
                245                 250                 255     
Pro Phe Ala Ser Leu Leu Lys Asn Leu Gly Thr Leu Phe Gly Leu Gly 
            260                 265                 270         
Leu Ala Leu Asn Ser Ser Met Tyr Arg Glu Ser Cys Lys Gly Lys Leu 
        275                 280                 285             
Ser Lys Trp Leu Pro Phe Arg Leu Ser Cys Ile Val Ala Ser Leu Val 
    290                 295                 300                 
Leu Leu His Val Phe Asp Ser Leu Lys Pro Pro Ser Gln Val Glu Leu 
305                 310                 315                 320 
Val Phe Tyr Val Leu Ser Phe Cys Lys Ser Ala Val Val Pro Leu Ala 
                325                 330                 335     
Ser Val Ser Val Ile Pro Tyr Cys Leu Ala Gln Val Leu Gly Gln Pro 
            340                 345                 350         
His Lys Lys Ser Leu 
        355         

<210> 39
<211> 357
<212> PRT
<213> Artificial Sequence


<220> 
<223> Modified G6Pase-a (A301V)

<400> 39
Met Glu Glu Gly Met Asn Val Leu His Asp Phe Gly Ile Gln Ser Thr 
1               5                   10                  15      
His Tyr Leu Gln Val Asn Tyr Gln Asp Ser Gln Asp Trp Phe Ile Leu 
            20                  25                  30          
Val Ser Val Ile Ala Asp Leu Arg Asn Ala Phe Tyr Val Leu Phe Pro 
        35                  40                  45              
Ile Trp Phe His Leu Gln Glu Ala Val Gly Ile Lys Leu Leu Trp Val 
    50                  55                  60                  
Ala Val Ile Gly Asp Trp Leu Asn Leu Val Phe Lys Trp Ile Leu Phe 
65                  70                  75                  80  
Gly Gln Arg Pro Tyr Trp Trp Val Leu Asp Thr Asp Tyr Tyr Ser Asn 
                85                  90                  95      
Thr Ser Val Pro Leu Ile Lys Gln Phe Pro Val Thr Cys Glu Thr Gly 
            100                 105                 110         
Pro Gly Ser Pro Ser Gly His Ala Met Gly Thr Ala Gly Val Tyr Tyr 
        115                 120                 125             
Val Met Val Thr Ser Thr Leu Ser Ile Phe Gln Gly Lys Ile Lys Pro 
    130                 135                 140                 
Thr Tyr Arg Phe Arg Cys Leu Asn Val Ile Leu Trp Leu Gly Phe Trp 
145                 150                 155                 160 
Ala Val Gln Leu Asn Val Cys Leu Ser Arg Ile Tyr Leu Ala Ala His 
                165                 170                 175     
Phe Pro His Gln Val Val Ala Gly Val Leu Ser Gly Ile Ala Val Ala 
            180                 185                 190         
Glu Thr Phe Ser His Ile His Ser Ile Tyr Asn Ala Ser Leu Lys Lys 
        195                 200                 205             
Tyr Phe Leu Ile Thr Phe Phe Leu Phe Ser Phe Ala Ile Gly Phe Tyr 
    210                 215                 220                 
Leu Leu Leu Lys Gly Leu Gly Val Asp Leu Leu Trp Thr Leu Glu Lys 
225                 230                 235                 240 
Ala Gln Arg Trp Cys Glu Gln Pro Glu Trp Val His Ile Asp Thr Thr 
                245                 250                 255     
Pro Phe Ala Ser Leu Leu Lys Asn Leu Gly Thr Leu Phe Gly Leu Gly 
            260                 265                 270         
Leu Ala Leu Asn Ser Ser Met Tyr Arg Glu Ser Cys Lys Gly Lys Leu 
        275                 280                 285             
Ser Lys Trp Leu Pro Phe Arg Leu Ser Ser Ile Val Val Ser Leu Val 
    290                 295                 300                 
Leu Leu His Val Phe Asp Ser Leu Lys Pro Pro Ser Gln Val Glu Leu 
305                 310                 315                 320 
Val Phe Tyr Val Leu Ser Phe Cys Lys Ser Ala Val Val Pro Leu Ala 
                325                 330                 335     
Ser Val Ser Val Ile Pro Tyr Cys Leu Ala Gln Val Leu Gly Gln Pro 
            340                 345                 350         
His Lys Lys Ser Leu 
        355         

<210> 40
<211> 357
<212> PRT
<213> Artificial Sequence


<220> 
<223> Modified G6Pase-a (V318T)

<400> 40
Met Glu Glu Gly Met Asn Val Leu His Asp Phe Gly Ile Gln Ser Thr 
1               5                   10                  15      
His Tyr Leu Gln Val Asn Tyr Gln Asp Ser Gln Asp Trp Phe Ile Leu 
            20                  25                  30          
Val Ser Val Ile Ala Asp Leu Arg Asn Ala Phe Tyr Val Leu Phe Pro 
        35                  40                  45              
Ile Trp Phe His Leu Gln Glu Ala Val Gly Ile Lys Leu Leu Trp Val 
    50                  55                  60                  
Ala Val Ile Gly Asp Trp Leu Asn Leu Val Phe Lys Trp Ile Leu Phe 
65                  70                  75                  80  
Gly Gln Arg Pro Tyr Trp Trp Val Leu Asp Thr Asp Tyr Tyr Ser Asn 
                85                  90                  95      
Thr Ser Val Pro Leu Ile Lys Gln Phe Pro Val Thr Cys Glu Thr Gly 
            100                 105                 110         
Pro Gly Ser Pro Ser Gly His Ala Met Gly Thr Ala Gly Val Tyr Tyr 
        115                 120                 125             
Val Met Val Thr Ser Thr Leu Ser Ile Phe Gln Gly Lys Ile Lys Pro 
    130                 135                 140                 
Thr Tyr Arg Phe Arg Cys Leu Asn Val Ile Leu Trp Leu Gly Phe Trp 
145                 150                 155                 160 
Ala Val Gln Leu Asn Val Cys Leu Ser Arg Ile Tyr Leu Ala Ala His 
                165                 170                 175     
Phe Pro His Gln Val Val Ala Gly Val Leu Ser Gly Ile Ala Val Ala 
            180                 185                 190         
Glu Thr Phe Ser His Ile His Ser Ile Tyr Asn Ala Ser Leu Lys Lys 
        195                 200                 205             
Tyr Phe Leu Ile Thr Phe Phe Leu Phe Ser Phe Ala Ile Gly Phe Tyr 
    210                 215                 220                 
Leu Leu Leu Lys Gly Leu Gly Val Asp Leu Leu Trp Thr Leu Glu Lys 
225                 230                 235                 240 
Ala Gln Arg Trp Cys Glu Gln Pro Glu Trp Val His Ile Asp Thr Thr 
                245                 250                 255     
Pro Phe Ala Ser Leu Leu Lys Asn Leu Gly Thr Leu Phe Gly Leu Gly 
            260                 265                 270         
Leu Ala Leu Asn Ser Ser Met Tyr Arg Glu Ser Cys Lys Gly Lys Leu 
        275                 280                 285             
Ser Lys Trp Leu Pro Phe Arg Leu Ser Ser Ile Val Ala Ser Leu Val 
    290                 295                 300                 
Leu Leu His Val Phe Asp Ser Leu Lys Pro Pro Ser Gln Thr Glu Leu 
305                 310                 315                 320 
Val Phe Tyr Val Leu Ser Phe Cys Lys Ser Ala Val Val Pro Leu Ala 
                325                 330                 335     
Ser Val Ser Val Ile Pro Tyr Cys Leu Ala Gln Val Leu Gly Gln Pro 
            340                 345                 350         
His Lys Lys Ser Leu 
        355         

<210> 41
<211> 357
<212> PRT
<213> Artificial Sequence


<220> 
<223> Modified G6Pase-a (V324T)

<400> 41
Met Glu Glu Gly Met Asn Val Leu His Asp Phe Gly Ile Gln Ser Thr 
1               5                   10                  15      
His Tyr Leu Gln Val Asn Tyr Gln Asp Ser Gln Asp Trp Phe Ile Leu 
            20                  25                  30          
Val Ser Val Ile Ala Asp Leu Arg Asn Ala Phe Tyr Val Leu Phe Pro 
        35                  40                  45              
Ile Trp Phe His Leu Gln Glu Ala Val Gly Ile Lys Leu Leu Trp Val 
    50                  55                  60                  
Ala Val Ile Gly Asp Trp Leu Asn Leu Val Phe Lys Trp Ile Leu Phe 
65                  70                  75                  80  
Gly Gln Arg Pro Tyr Trp Trp Val Leu Asp Thr Asp Tyr Tyr Ser Asn 
                85                  90                  95      
Thr Ser Val Pro Leu Ile Lys Gln Phe Pro Val Thr Cys Glu Thr Gly 
            100                 105                 110         
Pro Gly Ser Pro Ser Gly His Ala Met Gly Thr Ala Gly Val Tyr Tyr 
        115                 120                 125             
Val Met Val Thr Ser Thr Leu Ser Ile Phe Gln Gly Lys Ile Lys Pro 
    130                 135                 140                 
Thr Tyr Arg Phe Arg Cys Leu Asn Val Ile Leu Trp Leu Gly Phe Trp 
145                 150                 155                 160 
Ala Val Gln Leu Asn Val Cys Leu Ser Arg Ile Tyr Leu Ala Ala His 
                165                 170                 175     
Phe Pro His Gln Val Val Ala Gly Val Leu Ser Gly Ile Ala Val Ala 
            180                 185                 190         
Glu Thr Phe Ser His Ile His Ser Ile Tyr Asn Ala Ser Leu Lys Lys 
        195                 200                 205             
Tyr Phe Leu Ile Thr Phe Phe Leu Phe Ser Phe Ala Ile Gly Phe Tyr 
    210                 215                 220                 
Leu Leu Leu Lys Gly Leu Gly Val Asp Leu Leu Trp Thr Leu Glu Lys 
225                 230                 235                 240 
Ala Gln Arg Trp Cys Glu Gln Pro Glu Trp Val His Ile Asp Thr Thr 
                245                 250                 255     
Pro Phe Ala Ser Leu Leu Lys Asn Leu Gly Thr Leu Phe Gly Leu Gly 
            260                 265                 270         
Leu Ala Leu Asn Ser Ser Met Tyr Arg Glu Ser Cys Lys Gly Lys Leu 
        275                 280                 285             
Ser Lys Trp Leu Pro Phe Arg Leu Ser Ser Ile Val Ala Ser Leu Val 
    290                 295                 300                 
Leu Leu His Val Phe Asp Ser Leu Lys Pro Pro Ser Gln Val Glu Leu 
305                 310                 315                 320 
Val Phe Tyr Thr Leu Ser Phe Cys Lys Ser Ala Val Val Pro Leu Ala 
                325                 330                 335     
Ser Val Ser Val Ile Pro Tyr Cys Leu Ala Gln Val Leu Gly Gln Pro 
            340                 345                 350         
His Lys Lys Ser Leu 
        355         

<210> 42
<211> 357
<212> PRT
<213> Artificial Sequence


<220> 
<223> Modified G6Pase-a (V332A)

<400> 42
Met Glu Glu Gly Met Asn Val Leu His Asp Phe Gly Ile Gln Ser Thr 
1               5                   10                  15      
His Tyr Leu Gln Val Asn Tyr Gln Asp Ser Gln Asp Trp Phe Ile Leu 
            20                  25                  30          
Val Ser Val Ile Ala Asp Leu Arg Asn Ala Phe Tyr Val Leu Phe Pro 
        35                  40                  45              
Ile Trp Phe His Leu Gln Glu Ala Val Gly Ile Lys Leu Leu Trp Val 
    50                  55                  60                  
Ala Val Ile Gly Asp Trp Leu Asn Leu Val Phe Lys Trp Ile Leu Phe 
65                  70                  75                  80  
Gly Gln Arg Pro Tyr Trp Trp Val Leu Asp Thr Asp Tyr Tyr Ser Asn 
                85                  90                  95      
Thr Ser Val Pro Leu Ile Lys Gln Phe Pro Val Thr Cys Glu Thr Gly 
            100                 105                 110         
Pro Gly Ser Pro Ser Gly His Ala Met Gly Thr Ala Gly Val Tyr Tyr 
        115                 120                 125             
Val Met Val Thr Ser Thr Leu Ser Ile Phe Gln Gly Lys Ile Lys Pro 
    130                 135                 140                 
Thr Tyr Arg Phe Arg Cys Leu Asn Val Ile Leu Trp Leu Gly Phe Trp 
145                 150                 155                 160 
Ala Val Gln Leu Asn Val Cys Leu Ser Arg Ile Tyr Leu Ala Ala His 
                165                 170                 175     
Phe Pro His Gln Val Val Ala Gly Val Leu Ser Gly Ile Ala Val Ala 
            180                 185                 190         
Glu Thr Phe Ser His Ile His Ser Ile Tyr Asn Ala Ser Leu Lys Lys 
        195                 200                 205             
Tyr Phe Leu Ile Thr Phe Phe Leu Phe Ser Phe Ala Ile Gly Phe Tyr 
    210                 215                 220                 
Leu Leu Leu Lys Gly Leu Gly Val Asp Leu Leu Trp Thr Leu Glu Lys 
225                 230                 235                 240 
Ala Gln Arg Trp Cys Glu Gln Pro Glu Trp Val His Ile Asp Thr Thr 
                245                 250                 255     
Pro Phe Ala Ser Leu Leu Lys Asn Leu Gly Thr Leu Phe Gly Leu Gly 
            260                 265                 270         
Leu Ala Leu Asn Ser Ser Met Tyr Arg Glu Ser Cys Lys Gly Lys Leu 
        275                 280                 285             
Ser Lys Trp Leu Pro Phe Arg Leu Ser Ser Ile Val Ala Ser Leu Val 
    290                 295                 300                 
Leu Leu His Val Phe Asp Ser Leu Lys Pro Pro Ser Gln Val Glu Leu 
305                 310                 315                 320 
Val Phe Tyr Val Leu Ser Phe Cys Lys Ser Ala Ala Val Pro Leu Ala 
                325                 330                 335     
Ser Val Ser Val Ile Pro Tyr Cys Leu Ala Gln Val Leu Gly Gln Pro 
            340                 345                 350         
His Lys Lys Ser Leu 
        355         

<210> 43
<211> 357
<212> PRT
<213> Artificial Sequence


<220> 
<223> Modified G6Pase-a (Q347R)

<400> 43
Met Glu Glu Gly Met Asn Val Leu His Asp Phe Gly Ile Gln Ser Thr 
1               5                   10                  15      
His Tyr Leu Gln Val Asn Tyr Gln Asp Ser Gln Asp Trp Phe Ile Leu 
            20                  25                  30          
Val Ser Val Ile Ala Asp Leu Arg Asn Ala Phe Tyr Val Leu Phe Pro 
        35                  40                  45              
Ile Trp Phe His Leu Gln Glu Ala Val Gly Ile Lys Leu Leu Trp Val 
    50                  55                  60                  
Ala Val Ile Gly Asp Trp Leu Asn Leu Val Phe Lys Trp Ile Leu Phe 
65                  70                  75                  80  
Gly Gln Arg Pro Tyr Trp Trp Val Leu Asp Thr Asp Tyr Tyr Ser Asn 
                85                  90                  95      
Thr Ser Val Pro Leu Ile Lys Gln Phe Pro Val Thr Cys Glu Thr Gly 
            100                 105                 110         
Pro Gly Ser Pro Ser Gly His Ala Met Gly Thr Ala Gly Val Tyr Tyr 
        115                 120                 125             
Val Met Val Thr Ser Thr Leu Ser Ile Phe Gln Gly Lys Ile Lys Pro 
    130                 135                 140                 
Thr Tyr Arg Phe Arg Cys Leu Asn Val Ile Leu Trp Leu Gly Phe Trp 
145                 150                 155                 160 
Ala Val Gln Leu Asn Val Cys Leu Ser Arg Ile Tyr Leu Ala Ala His 
                165                 170                 175     
Phe Pro His Gln Val Val Ala Gly Val Leu Ser Gly Ile Ala Val Ala 
            180                 185                 190         
Glu Thr Phe Ser His Ile His Ser Ile Tyr Asn Ala Ser Leu Lys Lys 
        195                 200                 205             
Tyr Phe Leu Ile Thr Phe Phe Leu Phe Ser Phe Ala Ile Gly Phe Tyr 
    210                 215                 220                 
Leu Leu Leu Lys Gly Leu Gly Val Asp Leu Leu Trp Thr Leu Glu Lys 
225                 230                 235                 240 
Ala Gln Arg Trp Cys Glu Gln Pro Glu Trp Val His Ile Asp Thr Thr 
                245                 250                 255     
Pro Phe Ala Ser Leu Leu Lys Asn Leu Gly Thr Leu Phe Gly Leu Gly 
            260                 265                 270         
Leu Ala Leu Asn Ser Ser Met Tyr Arg Glu Ser Cys Lys Gly Lys Leu 
        275                 280                 285             
Ser Lys Trp Leu Pro Phe Arg Leu Ser Ser Ile Val Ala Ser Leu Val 
    290                 295                 300                 
Leu Leu His Val Phe Asp Ser Leu Lys Pro Pro Ser Gln Val Glu Leu 
305                 310                 315                 320 
Val Phe Tyr Val Leu Ser Phe Cys Lys Ser Ala Val Val Pro Leu Ala 
                325                 330                 335     
Ser Val Ser Val Ile Pro Tyr Cys Leu Ala Arg Val Leu Gly Gln Pro 
            340                 345                 350         
His Lys Lys Ser Leu 
        355         

<210> 44
<211> 357
<212> PRT
<213> Artificial Sequence


<220> 
<223> Modified G6Pase-a (L349F)

<400> 44
Met Glu Glu Gly Met Asn Val Leu His Asp Phe Gly Ile Gln Ser Thr 
1               5                   10                  15      
His Tyr Leu Gln Val Asn Tyr Gln Asp Ser Gln Asp Trp Phe Ile Leu 
            20                  25                  30          
Val Ser Val Ile Ala Asp Leu Arg Asn Ala Phe Tyr Val Leu Phe Pro 
        35                  40                  45              
Ile Trp Phe His Leu Gln Glu Ala Val Gly Ile Lys Leu Leu Trp Val 
    50                  55                  60                  
Ala Val Ile Gly Asp Trp Leu Asn Leu Val Phe Lys Trp Ile Leu Phe 
65                  70                  75                  80  
Gly Gln Arg Pro Tyr Trp Trp Val Leu Asp Thr Asp Tyr Tyr Ser Asn 
                85                  90                  95      
Thr Ser Val Pro Leu Ile Lys Gln Phe Pro Val Thr Cys Glu Thr Gly 
            100                 105                 110         
Pro Gly Ser Pro Ser Gly His Ala Met Gly Thr Ala Gly Val Tyr Tyr 
        115                 120                 125             
Val Met Val Thr Ser Thr Leu Ser Ile Phe Gln Gly Lys Ile Lys Pro 
    130                 135                 140                 
Thr Tyr Arg Phe Arg Cys Leu Asn Val Ile Leu Trp Leu Gly Phe Trp 
145                 150                 155                 160 
Ala Val Gln Leu Asn Val Cys Leu Ser Arg Ile Tyr Leu Ala Ala His 
                165                 170                 175     
Phe Pro His Gln Val Val Ala Gly Val Leu Ser Gly Ile Ala Val Ala 
            180                 185                 190         
Glu Thr Phe Ser His Ile His Ser Ile Tyr Asn Ala Ser Leu Lys Lys 
        195                 200                 205             
Tyr Phe Leu Ile Thr Phe Phe Leu Phe Ser Phe Ala Ile Gly Phe Tyr 
    210                 215                 220                 
Leu Leu Leu Lys Gly Leu Gly Val Asp Leu Leu Trp Thr Leu Glu Lys 
225                 230                 235                 240 
Ala Gln Arg Trp Cys Glu Gln Pro Glu Trp Val His Ile Asp Thr Thr 
                245                 250                 255     
Pro Phe Ala Ser Leu Leu Lys Asn Leu Gly Thr Leu Phe Gly Leu Gly 
            260                 265                 270         
Leu Ala Leu Asn Ser Ser Met Tyr Arg Glu Ser Cys Lys Gly Lys Leu 
        275                 280                 285             
Ser Lys Trp Leu Pro Phe Arg Leu Ser Ser Ile Val Ala Ser Leu Val 
    290                 295                 300                 
Leu Leu His Val Phe Asp Ser Leu Lys Pro Pro Ser Gln Val Glu Leu 
305                 310                 315                 320 
Val Phe Tyr Val Leu Ser Phe Cys Lys Ser Ala Val Val Pro Leu Ala 
                325                 330                 335     
Ser Val Ser Val Ile Pro Tyr Cys Leu Ala Gln Val Phe Gly Gln Pro 
            340                 345                 350         
His Lys Lys Ser Leu 
        355         

<210> 45
<211> 1074
<212> DNA
<213> Artificial Sequence


<220> 
<223> Codon optimised G6pc (co1) – used in example 5

<400> 45
atggaggaag gaatgaatgt tctccatgac tttgggatcc agtcaacaca ttacctccag      60

gtgaattacc aagactccca ggactggttc atcttggtgt ctgtgattgc agacctcagg     120

aatgccttct atgtcctctt ccccatctgg ttccatcttc aggaagctgt gggcattaaa     180

ctcctttggg tagctgtgat tggagactgg ctcaacctgg tctttaagtg gattctcttt     240

ggacagaggc catactggtg ggttttggat actgactact acagcaacac ttctgtgccc     300

ctgataaagc agttccctgt aacctgtgag actggaccag ggagcccctc tggccatgcc     360

atgggcacag caggtgtata ctatgtgatg gtcacatcta ctctttccat ctttcaggga     420

aagataaagc ccacctacag atttaggtgc ttgaatgtca ttttgtggtt gggattctgg     480

gctgtgcagc tgaatgtctg tctgtcaagg atctaccttg ctgctcattt tcctcatcaa     540

gttgttgctg gagtcctgtc aggcattgct gttgcagaaa ctttcagcca catccacagc     600

atctataatg ccagcctcaa gaaatatttt ctcattacct tcttcctgtt cagctttgcc     660

attggatttt atctgctgct caagggactg ggtgtagacc tcctgtggac tctggagaaa     720

gcccagaggt ggtgtgagca gccagaatgg gtccacattg acaccacacc ctttgccagc     780

ctcctcaaga acctgggcac cctctttggc ctggggctgg ctctcaactc cagcatgtac     840

agggagagct gcaaggggaa actcagcaag tggctcccat tcaggctcag ctctattgta     900

gcctccctgg tcctcctgca tgtctttgac tccttgaaac ccccatccca agtggagctg     960

gtcttctatg tcttgtcctt ctgcaagagt gctgtagtgc ccctggcatc tgtcagtgtc    1020

atcccctact gcctggccca ggtcctgggc cagccccaca agaagtcctt gtaa          1074


<210> 46
<211> 1074
<212> DNA
<213> Artificial Sequence


<220> 
<223> Codon optimised G6pc (co2) – used in example 5

<400> 46
atggaggagg ggatgaacgt gctgcacgac tttgggatcc agagcaccca ctacctgcag      60

gtgaactacc aggacagcca ggactggttt atcctggtgt ctgtgattgc tgacctgagg     120

aacgccttct acgtgctgtt ccctatctgg ttccacctgc aggaggctgt ggggatcaag     180

ctgctgtggg tggctgtgat tggggactgg ctgaacctgg tgttcaagtg gatcctgttt     240

ggccagaggc cctactggtg ggtgctggac acagactact acagcaacac ctctgtgccc     300

ctgatcaagc agttccctgt gacctgtgag acaggccctg gcagcccctc tggccacgct     360

atgggcacag ctggggtgta ctacgtgatg gtgaccagca ccctgtctat cttccagggc     420

aagatcaagc ccacctacag gttcaggtgc ctgaacgtga tcctgtggct gggcttctgg     480

gctgtgcagc tgaacgtgtg cctgagcagg atctacctgg ctgcccactt cccccaccag     540

gtggtggctg gggtgctgtc tgggattgct gtggctgaga ccttcagcca catccactct     600

atctacaacg ccagcctgaa gaagtacttc ctgatcacct tcttcctgtt cagctttgct     660

attggcttct acctgctgct gaagggcctg ggggtggacc tgctgtggac cctggagaag     720

gcccagaggt ggtgtgagca gcctgagtgg gtgcacattg acaccacccc ctttgccagc     780

ctgctgaaga acctgggcac cctgtttggc ctgggcctgg ccctgaacag ctctatgtac     840

agggagagct gcaagggcaa gctgagcaag tggctgccct tcaggctgag ctctattgtg     900

gccagcctgg tgctgctgca cgtgtttgac agcctgaagc cccccagcca ggtggagctg     960

gtgttctacg tgctgagctt ctgcaagtct gctgtggtgc ccctggcctc tgtgtctgtg    1020

atcccctact gcctggccca ggtgctgggc cagccccaca agaagagcct gtag          1074


<210> 47
<211> 441
<212> DNA
<213> Artificial Sequence


<220> 
<223> Codon optimised intron of the β-globin gene (HBB2 co) – used in
      examples 4 and 5

<400> 47
gtacacatat tgaccaaatc agggtaattt tgcatttgta attttaaaaa atgctttctt      60

cttttaatat acttttttgt ttatcttatt tctaatactt tccctaatct ctttctttca     120

gggcaataat gatacaatgt atcatgcctc tttgcaccat tctaaagaat aacagtgata     180

atttctgggt taaggcaata gcaatatttc tgcatataaa tatttctgca tataaattgt     240

aactgatgta agaggtttca tattgctaat agcagctaca atccagctac cattctgctt     300

ttattttctg gttgggataa ggctggatta ttctgagtcc aagctaggcc cttttgctaa     360

tcttgttcat acctcttatc ttcctcccac agctcctggg caacctgctg gtctctctgc     420

tggcccatca ctttggcaaa g                                               441


<210> 48
<211> 3134
<212> DNA
<213> Artificial Sequence


<220> 
<223> hAAT_HBB2Co_hG6pc wt co1 (expression cassette used in example 5)

<400> 48
aaggctcaga ggcacacagg agtttctggg ctcaccctgc ccccttccaa cccctcagtt      60

cccatcctcc agcagctgtt tgtgtgctgc ctctgaagtc cacactgaac aaacttcagc     120

ctactcatgt ccctaaaatg ggcaaacatt gcaagcagca aacagcaaac acacagccct     180

ccctgcctgc tgaccttgga gctggggcag aggtcagaga cctctctggg cccatgccac     240

ctccaacatc cactcgaccc cttggaattt cggtggagag gagcagaggt tgtcctggcg     300

tggtttaggt agtgtgagag gggtacccgg ggatcttgct accagtggaa cagccactaa     360

ggattctgca gtgagagcag agggccagct aagtggtact ctcccagaga ctgtctgact     420

cacgccaccc cctccacctt ggacacagga cgctgtggtt tctgagccag gtacaatgac     480

tcctttcggt aagtgcagtg gaagctgtac actgcccagg caaagcgtcc gggcagcgta     540

ggcgggcgac tcagatccca gccagtggac ttagcccctg tttgctcctc cgataactgg     600

ggtgaccttg gttaatattc accagcagcc tcccccgttg cccctctgga tccactgctt     660

aaatacggac gaggacaggg ccctgtctcc tcagcttcag gcaccaccac tgacctggga     720

cagtgaatag atcctgagaa cttcagggtg agtctatggg acccttgatg ttttctttcc     780

ccttcttttc tatggttaag ttcatgtcat aggaagggga gaagtaacag ggtacacata     840

ttgaccaaat cagggtaatt ttgcatttgt aattttaaaa aatgctttct tcttttaata     900

tacttttttg tttatcttat ttctaatact ttccctaatc tctttctttc agggcaataa     960

tgatacaatg tatcatgcct ctttgcacca ttctaaagaa taacagtgat aatttctggg    1020

ttaaggcaat agcaatattt ctgcatataa atatttctgc atataaattg taactgatgt    1080

aagaggtttc atattgctaa tagcagctac aatccagcta ccattctgct tttattttct    1140

ggttgggata aggctggatt attctgagtc caagctaggc ccttttgcta atcttgttca    1200

tacctcttat cttcctccca cagctcctgg gcaacctgct ggtctctctg ctggcccatc    1260

actttggcaa agcacgcgtg ccaccatgga ggaaggaatg aatgttctcc atgactttgg    1320

gatccagtca acacattacc tccaggtgaa ttaccaagac tcccaggact ggttcatctt    1380

ggtgtctgtg attgcagacc tcaggaatgc cttctatgtc ctcttcccca tctggttcca    1440

tcttcaggaa gctgtgggca ttaaactcct ttgggtagct gtgattggag actggctcaa    1500

cctggtcttt aagtggattc tctttggaca gaggccatac tggtgggttt tggatactga    1560

ctactacagc aacacttctg tgcccctgat aaagcagttc cctgtaacct gtgagactgg    1620

accagggagc ccctctggcc atgccatggg cacagcaggt gtatactatg tgatggtcac    1680

atctactctt tccatctttc agggaaagat aaagcccacc tacagattta ggtgcttgaa    1740

tgtcattttg tggttgggat tctgggctgt gcagctgaat gtctgtctgt caaggatcta    1800

ccttgctgct cattttcctc atcaagttgt tgctggagtc ctgtcaggca ttgctgttgc    1860

agaaactttc agccacatcc acagcatcta taatgccagc ctcaagaaat attttctcat    1920

taccttcttc ctgttcagct ttgccattgg attttatctg ctgctcaagg gactgggtgt    1980

agacctcctg tggactctgg agaaagccca gaggtggtgt gagcagccag aatgggtcca    2040

cattgacacc acaccctttg ccagcctcct caagaacctg ggcaccctct ttggcctggg    2100

gctggctctc aactccagca tgtacaggga gagctgcaag gggaaactca gcaagtggct    2160

cccattcagg ctcagctcta ttgtagcctc cctggtcctc ctgcatgtct ttgactcctt    2220

gaaaccccca tcccaagtgg agctggtctt ctatgtcttg tccttctgca agagtgctgt    2280

agtgcccctg gcatctgtca gtgtcatccc ctactgcctg gcccaggtcc tgggccagcc    2340

ccacaagaag tccttgtaac tcgaggaatt caccccacca gtgcaggctg cctatcagaa    2400

agtggtggct ggtgtggcta atgccctggc ccacaagtat cactaagctc gctttcttgc    2460

tgtccaattt ctattaaagg ttcctttgtt ccctaagtcc aactactaaa ctgggggata    2520

ttatgaaggg ccttgagcat ctggattctg cctaataaaa aacatttatt ttcattgcaa    2580

tgatgtattt aaattatttc tgaatatttt actaaaaagg gaatgtggga ggtcagtgca    2640

tttaaaacat aaagaaatga agagctagtt caaaccttgg gaaaatacac tatatcttaa    2700

actccatgaa agaaggtgag gctgcaaaca gctaatgcac attggcaaca gcccctgatg    2760

cctatgcctt attcatccct cagaaaagga ttcaagtaga ggcttgattt ggaggttaaa    2820

gttttgctat gctgtatttt acattactta ttgttttagc tgtcctcatg aatgtctttt    2880

cactacccat ttgcttatcc tgcatctctc agccttgact ccactcagtt ctcttgctta    2940

gagataccac ctttcccctg aagtgttcct tccatgtttt acggcgagat ggtttctcct    3000

cgcctggcca ctcagcctta gttgtctctg ttgtcttata gaggtctact tgaagaagga    3060

aaaacagggg gcatggtttg actgtcctgt gagcccttct tccctgcctc ccccactcac    3120

agtgacccgg aatc                                                      3134


<210> 49
<211> 3134
<212> DNA
<213> Artificial Sequence


<220> 
<223> hAAT_HBB2Co_hG6pc wt co2 (expression cassette used in example 5)

<400> 49
aaggctcaga ggcacacagg agtttctggg ctcaccctgc ccccttccaa cccctcagtt      60

cccatcctcc agcagctgtt tgtgtgctgc ctctgaagtc cacactgaac aaacttcagc     120

ctactcatgt ccctaaaatg ggcaaacatt gcaagcagca aacagcaaac acacagccct     180

ccctgcctgc tgaccttgga gctggggcag aggtcagaga cctctctggg cccatgccac     240

ctccaacatc cactcgaccc cttggaattt cggtggagag gagcagaggt tgtcctggcg     300

tggtttaggt agtgtgagag gggtacccgg ggatcttgct accagtggaa cagccactaa     360

ggattctgca gtgagagcag agggccagct aagtggtact ctcccagaga ctgtctgact     420

cacgccaccc cctccacctt ggacacagga cgctgtggtt tctgagccag gtacaatgac     480

tcctttcggt aagtgcagtg gaagctgtac actgcccagg caaagcgtcc gggcagcgta     540

ggcgggcgac tcagatccca gccagtggac ttagcccctg tttgctcctc cgataactgg     600

ggtgaccttg gttaatattc accagcagcc tcccccgttg cccctctgga tccactgctt     660

aaatacggac gaggacaggg ccctgtctcc tcagcttcag gcaccaccac tgacctggga     720

cagtgaatag atcctgagaa cttcagggtg agtctatggg acccttgatg ttttctttcc     780

ccttcttttc tatggttaag ttcatgtcat aggaagggga gaagtaacag ggtacacata     840

ttgaccaaat cagggtaatt ttgcatttgt aattttaaaa aatgctttct tcttttaata     900

tacttttttg tttatcttat ttctaatact ttccctaatc tctttctttc agggcaataa     960

tgatacaatg tatcatgcct ctttgcacca ttctaaagaa taacagtgat aatttctggg    1020

ttaaggcaat agcaatattt ctgcatataa atatttctgc atataaattg taactgatgt    1080

aagaggtttc atattgctaa tagcagctac aatccagcta ccattctgct tttattttct    1140

ggttgggata aggctggatt attctgagtc caagctaggc ccttttgcta atcttgttca    1200

tacctcttat cttcctccca cagctcctgg gcaacctgct ggtctctctg ctggcccatc    1260

actttggcaa agcacgcgtg ccaccatgga ggaggggatg aacgtgctgc acgactttgg    1320

gatccagagc acccactacc tgcaggtgaa ctaccaggac agccaggact ggtttatcct    1380

ggtgtctgtg attgctgacc tgaggaacgc cttctacgtg ctgttcccta tctggttcca    1440

cctgcaggag gctgtgggga tcaagctgct gtgggtggct gtgattgggg actggctgaa    1500

cctggtgttc aagtggatcc tgtttggcca gaggccctac tggtgggtgc tggacacaga    1560

ctactacagc aacacctctg tgcccctgat caagcagttc cctgtgacct gtgagacagg    1620

ccctggcagc ccctctggcc acgctatggg cacagctggg gtgtactacg tgatggtgac    1680

cagcaccctg tctatcttcc agggcaagat caagcccacc tacaggttca ggtgcctgaa    1740

cgtgatcctg tggctgggct tctgggctgt gcagctgaac gtgtgcctga gcaggatcta    1800

cctggctgcc cacttccccc accaggtggt ggctggggtg ctgtctggga ttgctgtggc    1860

tgagaccttc agccacatcc actctatcta caacgccagc ctgaagaagt acttcctgat    1920

caccttcttc ctgttcagct ttgctattgg cttctacctg ctgctgaagg gcctgggggt    1980

ggacctgctg tggaccctgg agaaggccca gaggtggtgt gagcagcctg agtgggtgca    2040

cattgacacc accccctttg ccagcctgct gaagaacctg ggcaccctgt ttggcctggg    2100

cctggccctg aacagctcta tgtacaggga gagctgcaag ggcaagctga gcaagtggct    2160

gcccttcagg ctgagctcta ttgtggccag cctggtgctg ctgcacgtgt ttgacagcct    2220

gaagcccccc agccaggtgg agctggtgtt ctacgtgctg agcttctgca agtctgctgt    2280

ggtgcccctg gcctctgtgt ctgtgatccc ctactgcctg gcccaggtgc tgggccagcc    2340

ccacaagaag agcctgtagc tcgaggaatt caccccacca gtgcaggctg cctatcagaa    2400

agtggtggct ggtgtggcta atgccctggc ccacaagtat cactaagctc gctttcttgc    2460

tgtccaattt ctattaaagg ttcctttgtt ccctaagtcc aactactaaa ctgggggata    2520

ttatgaaggg ccttgagcat ctggattctg cctaataaaa aacatttatt ttcattgcaa    2580

tgatgtattt aaattatttc tgaatatttt actaaaaagg gaatgtggga ggtcagtgca    2640

tttaaaacat aaagaaatga agagctagtt caaaccttgg gaaaatacac tatatcttaa    2700

actccatgaa agaaggtgag gctgcaaaca gctaatgcac attggcaaca gcccctgatg    2760

cctatgcctt attcatccct cagaaaagga ttcaagtaga ggcttgattt ggaggttaaa    2820

gttttgctat gctgtatttt acattactta ttgttttagc tgtcctcatg aatgtctttt    2880

cactacccat ttgcttatcc tgcatctctc agccttgact ccactcagtt ctcttgctta    2940

gagataccac ctttcccctg aagtgttcct tccatgtttt acggcgagat ggtttctcct    3000

cgcctggcca ctcagcctta gttgtctctg ttgtcttata gaggtctact tgaagaagga    3060

aaaacagggg gcatggtttg actgtcctgt gagcccttct tccctgcctc ccccactcac    3120

agtgacccgg aatc                                                      3134


<210> 50
<211> 3134
<212> DNA
<213> Artificial Sequence


<220> 
<223> hAAT_HBB2Co_hG6pc wt co3 (expression cassette used in example 5)
      - comprising SEQ ID NO: 3

<400> 50
aaggctcaga ggcacacagg agtttctggg ctcaccctgc ccccttccaa cccctcagtt      60

cccatcctcc agcagctgtt tgtgtgctgc ctctgaagtc cacactgaac aaacttcagc     120

ctactcatgt ccctaaaatg ggcaaacatt gcaagcagca aacagcaaac acacagccct     180

ccctgcctgc tgaccttgga gctggggcag aggtcagaga cctctctggg cccatgccac     240

ctccaacatc cactcgaccc cttggaattt cggtggagag gagcagaggt tgtcctggcg     300

tggtttaggt agtgtgagag gggtacccgg ggatcttgct accagtggaa cagccactaa     360

ggattctgca gtgagagcag agggccagct aagtggtact ctcccagaga ctgtctgact     420

cacgccaccc cctccacctt ggacacagga cgctgtggtt tctgagccag gtacaatgac     480

tcctttcggt aagtgcagtg gaagctgtac actgcccagg caaagcgtcc gggcagcgta     540

ggcgggcgac tcagatccca gccagtggac ttagcccctg tttgctcctc cgataactgg     600

ggtgaccttg gttaatattc accagcagcc tcccccgttg cccctctgga tccactgctt     660

aaatacggac gaggacaggg ccctgtctcc tcagcttcag gcaccaccac tgacctggga     720

cagtgaatag atcctgagaa cttcagggtg agtctatggg acccttgatg ttttctttcc     780

ccttcttttc tatggttaag ttcatgtcat aggaagggga gaagtaacag ggtacacata     840

ttgaccaaat cagggtaatt ttgcatttgt aattttaaaa aatgctttct tcttttaata     900

tacttttttg tttatcttat ttctaatact ttccctaatc tctttctttc agggcaatat     960

tgatacaatg tatcttgcct ctttgcacca ttctaaagaa taacagtgat aatttctggg    1020

ttaaggcaat agcaatattt ctgcatataa atatttctgc atataaattg taactgatgt    1080

aagaggtttc atattgctaa tagcagctac aatccagcta ccattctgct tttattttct    1140

ggttgggata aggctggatt attctgagtc caagctaggc ccttttgcta atcttgttca    1200

tacctcttat cttcctccca cagctcctgg gcaacctgct ggtctctctg ctggcccatc    1260

actttggcaa agcacgcgtg ccaccatgga ggagggaatg aatgtgctgc acgacttcgg    1320

gattcagtct acccactatc tccaagtcaa ttatcaagac agtcaagatt ggttcatcct    1380

cgtctccgtt atagctgacc tcaggaatgc tttttatgta ctgttcccaa tatggtttca    1440

tctgcaagaa gcagttggaa ttaaactgct gtgggtggcc gtaatcggag actggttgaa    1500

cctggtgttc aaatggatcc tttttggtca gaggccatat tggtgggtct tggacaccga    1560

ctattatagc aacacatctg tacccctgat aaagcaattc cctgtaacgt gtgaaactgg    1620

gcctgggtca cccagtggac acgcgatggg gactgccggg gtttactacg taatggttac    1680

tagcacgctt agcattttcc agggcaagat caaaccgact tatcgcttcc gatgcctcaa    1740

tgtgatcctc tggctgggat tctgggcggt tcaactgaac gtatgcctct cacggattta    1800

tctggctgca catttcccgc atcaagtagt ggctggtgtg ttgtctggca tagcagtggc    1860

tgaaacattc tcacacattc attctattta caacgcttca ttgaaaaaat actttctgat    1920

tacttttttt cttttcagct ttgcaattgg cttttacttg ctgcttaaag gtcttggcgt    1980

cgacctgctc tggactcttg agaaagcgca acgctggtgc gaacaacccg agtgggtcca    2040

catagatacg accccgttcg cgtctcttct gaaaaatctc ggtaccctct tcggactggg    2100

acttgctttg aattcttcaa tgtatcggga atcctgcaag ggcaaactgt ccaagtggtt    2160

gccctttcgc ctttcaagca ttgtcgcttc ccttgtgctc ctgcacgtct tcgactcact    2220

gaagccgccg agccaagtcg agctcgtgtt ttacgtgttg tctttctgta aaagtgccgt    2280

cgtgccactc gccagtgtga gcgtgatacc ttattgcctc gcacaggtgc ttgggcagcc    2340

tcataagaag tctctgtaaa gatctgaatt caccccacca gtgcaggctg cctatcagaa    2400

agtggtggct ggtgtggcta atgccctggc ccacaagtat cactaagctc gctttcttgc    2460

tgtccaattt ctattaaagg ttcctttgtt ccctaagtcc aactactaaa ctgggggata    2520

ttatgaaggg ccttgagcat ctggattctg cctaataaaa aacatttatt ttcattgcaa    2580

tgatgtattt aaattatttc tgaatatttt actaaaaagg gaatgtggga ggtcagtgca    2640

tttaaaacat aaagaaatga agagctagtt caaaccttgg gaaaatacac tatatcttaa    2700

actccatgaa agaaggtgag gctgcaaaca gctaatgcac attggcaaca gcccctgatg    2760

cctatgcctt attcatccct cagaaaagga ttcaagtaga ggcttgattt ggaggttaaa    2820

gtttggctat gctgtatttt acattactta ttgttttagc tgtcctcatg aatgtctttt    2880

cactacccat ttgcttatcc tgcatctctc agccttgact ccactcagtt ctcttgctta    2940

gagataccac ctttcccctg aagtgttcct tccatgtttt acggcgagat ggtttctcct    3000

cgcctggcca ctcagcctta gttgtctctg ttgtcttata gaggtctact tgaagaagga    3060

aaaacagggg gcatggtttg actgtcctgt gagcccttct tccctgcctc ccccactcac    3120

agtgacccgg aatc                                                      3134


<210> 51
<211> 3134
<212> DNA
<213> Artificial Sequence


<220> 
<223> hAAT_HBB2 co_hG6pc mut (expression cassette) Comprising SEQ ID
      NO: 5

<400> 51
aaggctcaga ggcacacagg agtttctggg ctcaccctgc ccccttccaa cccctcagtt      60

cccatcctcc agcagctgtt tgtgtgctgc ctctgaagtc cacactgaac aaacttcagc     120

ctactcatgt ccctaaaatg ggcaaacatt gcaagcagca aacagcaaac acacagccct     180

ccctgcctgc tgaccttgga gctggggcag aggtcagaga cctctctggg cccatgccac     240

ctccaacatc cactcgaccc cttggaattt cggtggagag gagcagaggt tgtcctggcg     300

tggtttaggt agtgtgagag gggtacccgg ggatcttgct accagtggaa cagccactaa     360

ggattctgca gtgagagcag agggccagct aagtggtact ctcccagaga ctgtctgact     420

cacgccaccc cctccacctt ggacacagga cgctgtggtt tctgagccag gtacaatgac     480

tcctttcggt aagtgcagtg gaagctgtac actgcccagg caaagcgtcc gggcagcgta     540

ggcgggcgac tcagatccca gccagtggac ttagcccctg tttgctcctc cgataactgg     600

ggtgaccttg gttaatattc accagcagcc tcccccgttg cccctctgga tccactgctt     660

aaatacggac gaggacaggg ccctgtctcc tcagcttcag gcaccaccac tgacctggga     720

cagtgaatag atcctgagaa cttcagggtg agtctatggg acccttgatg ttttctttcc     780

ccttcttttc tatggttaag ttcatgtcat aggaagggga gaagtaacag ggtacacata     840

ttgaccaaat cagggtaatt ttgcatttgt aattttaaaa aatgctttct tcttttaata     900

tacttttttg tttatcttat ttctaatact ttccctaatc tctttctttc agggcaataa     960

tgatacaatg tatcatgcct ctttgcacca ttctaaagaa taacagtgat aatttctggg    1020

ttaaggcaat agcaatattt ctgcatataa atatttctgc atataaattg taactgatgt    1080

aagaggtttc atattgctaa tagcagctac aatccagcta ccattctgct tttattttct    1140

ggttgggata aggctggatt attctgagtc caagctaggc ccttttgcta atcttgttca    1200

tacctcttat cttcctccca cagctcctgg gcaacctgct ggtctctctg ctggcccatc    1260

actttggcaa agcacgcgtg ccaccatgga ggaaggaatg aatgttctcc atgactttgg    1320

gatccagtca acacattacc tccaggtgaa ttaccaagac tcccaggact ggttcatctt    1380

ggtgtccgtg atcgcagacc tcaggaatgc cttctacgtc ctcttcccca tctggttcca    1440

tcttcaggaa gctgtgggca ttaaactcct ttgggtagct gtgattggag actggctcaa    1500

cctcgtcttt aagtggattc tctttggaca gcgtccatac tggtgggttt tggatactga    1560

ctactacagc aacacttccg tgcccctgat aaagcagttc cctgtaacct gtgagactgg    1620

accagggagc ccctctggcc atgccatggg cacagcaggt gtatactacg tgatggtcac    1680

atctactctt tccatctttc agggaaagat aaagccgacc tacagatttc ggtgcttgaa    1740

tgtcattttg tggttgggat tctgggctgt gcagctgaat gtctgtctgt cacgaatcta    1800

ccttgctgct cattttcctc atcaagttgt tgctggagtc ctgtcaggca ttgctgttgc    1860

agaaactttc agccacatcc acagcatcta taatgccagc ctcaagaaat attttctcat    1920

taccttcttc ctgttcagct tcgccatcgg attttatctg ctgctcaagg gactgggtgt    1980

agacctcctg tggactctgg agaaagccca gaggtggtgc gagcagccag aatgggtcca    2040

cattgacacc acaccctttg ccagcctcct caagaacctg ggcacgctct ttggcctggg    2100

gctggctctc aactccagca tgtacaggga gagctgcaag gggaaactca gcaagtggct    2160

cccattccgc ctcagctgta ttgtagcctc cctcgtcctc ctgcacgtct ttgactcctt    2220

gaaaccccca tcccaagtcg agctggtctt ctacgtcttg tccttctgca agagtgcggt    2280

agtgcccctg gcatccgtca gtgtcatccc ctactgcctc gcccaggtcc tgggccagcc    2340

gcacaagaag tcgttgtaac tcgaggaatt caccccacca gtgcaggctg cctatcagaa    2400

agtggtggct ggtgtggcta atgccctggc ccacaagtat cactaagctc gctttcttgc    2460

tgtccaattt ctattaaagg ttcctttgtt ccctaagtcc aactactaaa ctgggggata    2520

ttatgaaggg ccttgagcat ctggattctg cctaataaaa aacatttatt ttcattgcaa    2580

tgatgtattt aaattatttc tgaatatttt actaaaaagg gaatgtggga ggtcagtgca    2640

tttaaaacat aaagaaatga agagctagtt caaaccttgg gaaaatacac tatatcttaa    2700

actccatgaa agaaggtgag gctgcaaaca gctaatgcac attggcaaca gcccctgatg    2760

cctatgcctt attcatccct cagaaaagga ttcaagtaga ggcttgattt ggaggttaaa    2820

gttttgctat gctgtatttt acattactta ttgttttagc tgtcctcatg aatgtctttt    2880

cactacccat ttgcttatcc tgcatctctc agccttgact ccactcagtt ctcttgctta    2940

gagataccac ctttcccctg aagtgttcct tccatgtttt acggcgagat ggtttctcct    3000

cgcctggcca ctcagcctta gttgtctctg ttgtcttata gaggtctact tgaagaagga    3060

aaaacagggg gcatggtttg actgtcctgt gagcccttct tccctgcctc ccccactcac    3120

agtgacccgg aatc                                                      3134


<210> 52
<211> 1074
<212> DNA
<213> Artificial Sequence


<220> 
<223> Codon optimized hG6pc mut (co1)

<400> 52
atggaggaag gaatgaatgt tctccatgac tttgggatcc agtcaacaca ttacctccag      60

gtgaattacc aagactccca ggactggttc atcttggtgt ctgtgattgc agacctcagg     120

aatgccttct atgtcctctt ccccatctgg ttccatcttc aggaagctgt gggcattaaa     180

ctcctttggg tagctgtgat tggagactgg ctcaacctgg tctttaagtg gattctcttt     240

ggacagaggc catactggtg ggttttggat actgactact acagcaacac ttctgtgccc     300

ctgataaagc agttccctgt aacctgtgag actggaccag ggagcccctc tggccatgcc     360

atgggcacag caggtgtata ctatgtgatg gtcacatcta ctctttccat ctttcaggga     420

aagataaagc ccacctacag atttaggtgc ttgaatgtca ttttgtggtt gggattctgg     480

gctgtgcagc tgaatgtctg tctgtcaagg atctaccttg ctgctcattt tcctcatcaa     540

gttgttgctg gagtcctgtc aggcattgct gttgcagaaa ctttcagcca catccacagc     600

atctataatg ccagcctcaa gaaatatttt ctcattacct tcttcctgtt cagctttgcc     660

attggatttt atctgctgct caagggactg ggtgtagacc tcctgtggac tctggagaaa     720

gcccagaggt ggtgtgagca gccagaatgg gtccacattg acaccacacc ctttgccagc     780

ctcctcaaga acctgggcac cctctttggc ctggggctgg ctctcaactc cagcatgtac     840

agggagagct gcaaggggaa actcagcaag tggctcccat tcaggctcag ctgtattgta     900

gcctccctgg tcctcctgca tgtctttgac tccttgaaac ccccatccca agtggagctg     960

gtcttctatg tcttgtcctt ctgcaagagt gctgtagtgc ccctggcatc tgtcagtgtc    1020

atcccctact gcctggccca ggtcctgggc cagccccaca agaagtcctt gtaa          1074


<210> 53
<211> 3134
<212> DNA
<213> Artificial Sequence


<220> 
<223> hAAT_HBB2 co_hG6pc mut co1 (expression cassette)

<400> 53
aaggctcaga ggcacacagg agtttctggg ctcaccctgc ccccttccaa cccctcagtt      60

cccatcctcc agcagctgtt tgtgtgctgc ctctgaagtc cacactgaac aaacttcagc     120

ctactcatgt ccctaaaatg ggcaaacatt gcaagcagca aacagcaaac acacagccct     180

ccctgcctgc tgaccttgga gctggggcag aggtcagaga cctctctggg cccatgccac     240

ctccaacatc cactcgaccc cttggaattt cggtggagag gagcagaggt tgtcctggcg     300

tggtttaggt agtgtgagag gggtacccgg ggatcttgct accagtggaa cagccactaa     360

ggattctgca gtgagagcag agggccagct aagtggtact ctcccagaga ctgtctgact     420

cacgccaccc cctccacctt ggacacagga cgctgtggtt tctgagccag gtacaatgac     480

tcctttcggt aagtgcagtg gaagctgtac actgcccagg caaagcgtcc gggcagcgta     540

ggcgggcgac tcagatccca gccagtggac ttagcccctg tttgctcctc cgataactgg     600

ggtgaccttg gttaatattc accagcagcc tcccccgttg cccctctgga tccactgctt     660

aaatacggac gaggacaggg ccctgtctcc tcagcttcag gcaccaccac tgacctggga     720

cagtgaatag atcctgagaa cttcagggtg agtctatggg acccttgatg ttttctttcc     780

ccttcttttc tatggttaag ttcatgtcat aggaagggga gaagtaacag ggtacacata     840

ttgaccaaat cagggtaatt ttgcatttgt aattttaaaa aatgctttct tcttttaata     900

tacttttttg tttatcttat ttctaatact ttccctaatc tctttctttc agggcaataa     960

tgatacaatg tatcatgcct ctttgcacca ttctaaagaa taacagtgat aatttctggg    1020

ttaaggcaat agcaatattt ctgcatataa atatttctgc atataaattg taactgatgt    1080

aagaggtttc atattgctaa tagcagctac aatccagcta ccattctgct tttattttct    1140

ggttgggata aggctggatt attctgagtc caagctaggc ccttttgcta atcttgttca    1200

tacctcttat cttcctccca cagctcctgg gcaacctgct ggtctctctg ctggcccatc    1260

actttggcaa agcacgcgtg ccaccatgga ggaaggaatg aatgttctcc atgactttgg    1320

gatccagtca acacattacc tccaggtgaa ttaccaagac tcccaggact ggttcatctt    1380

ggtgtctgtg attgcagacc tcaggaatgc cttctatgtc ctcttcccca tctggttcca    1440

tcttcaggaa gctgtgggca ttaaactcct ttgggtagct gtgattggag actggctcaa    1500

cctggtcttt aagtggattc tctttggaca gaggccatac tggtgggttt tggatactga    1560

ctactacagc aacacttctg tgcccctgat aaagcagttc cctgtaacct gtgagactgg    1620

accagggagc ccctctggcc atgccatggg cacagcaggt gtatactatg tgatggtcac    1680

atctactctt tccatctttc agggaaagat aaagcccacc tacagattta ggtgcttgaa    1740

tgtcattttg tggttgggat tctgggctgt gcagctgaat gtctgtctgt caaggatcta    1800

ccttgctgct cattttcctc atcaagttgt tgctggagtc ctgtcaggca ttgctgttgc    1860

agaaactttc agccacatcc acagcatcta taatgccagc ctcaagaaat attttctcat    1920

taccttcttc ctgttcagct ttgccattgg attttatctg ctgctcaagg gactgggtgt    1980

agacctcctg tggactctgg agaaagccca gaggtggtgt gagcagccag aatgggtcca    2040

cattgacacc acaccctttg ccagcctcct caagaacctg ggcaccctct ttggcctggg    2100

gctggctctc aactccagca tgtacaggga gagctgcaag gggaaactca gcaagtggct    2160

cccattcagg ctcagctgta ttgtagcctc cctggtcctc ctgcatgtct ttgactcctt    2220

gaaaccccca tcccaagtgg agctggtctt ctatgtcttg tccttctgca agagtgctgt    2280

agtgcccctg gcatctgtca gtgtcatccc ctactgcctg gcccaggtcc tgggccagcc    2340

ccacaagaag tccttgtaac tcgaggaatt caccccacca gtgcaggctg cctatcagaa    2400

agtggtggct ggtgtggcta atgccctggc ccacaagtat cactaagctc gctttcttgc    2460

tgtccaattt ctattaaagg ttcctttgtt ccctaagtcc aactactaaa ctgggggata    2520

ttatgaaggg ccttgagcat ctggattctg cctaataaaa aacatttatt ttcattgcaa    2580

tgatgtattt aaattatttc tgaatatttt actaaaaagg gaatgtggga ggtcagtgca    2640

tttaaaacat aaagaaatga agagctagtt caaaccttgg gaaaatacac tatatcttaa    2700

actccatgaa agaaggtgag gctgcaaaca gctaatgcac attggcaaca gcccctgatg    2760

cctatgcctt attcatccct cagaaaagga ttcaagtaga ggcttgattt ggaggttaaa    2820

gttttgctat gctgtatttt acattactta ttgttttagc tgtcctcatg aatgtctttt    2880

cactacccat ttgcttatcc tgcatctctc agccttgact ccactcagtt ctcttgctta    2940

gagataccac ctttcccctg aagtgttcct tccatgtttt acggcgagat ggtttctcct    3000

cgcctggcca ctcagcctta gttgtctctg ttgtcttata gaggtctact tgaagaagga    3060

aaaacagggg gcatggtttg actgtcctgt gagcccttct tccctgcctc ccccactcac    3120

agtgacccgg aatc                                                      3134


<210> 54
<211> 1074
<212> DNA
<213> Artificial Sequence


<220> 
<223> Codon optimized hG6pc mut (co2)

<400> 54
atggaggagg ggatgaacgt gctgcacgac tttgggatcc agagcaccca ctacctgcag      60

gtgaactacc aggacagcca ggactggttt atcctggtgt ctgtgattgc tgacctgagg     120

aacgccttct acgtgctgtt ccctatctgg ttccacctgc aggaggctgt ggggatcaag     180

ctgctgtggg tggctgtgat tggggactgg ctgaacctgg tgttcaagtg gatcctgttt     240

ggccagaggc cctactggtg ggtgctggac acagactact acagcaacac ctctgtgccc     300

ctgatcaagc agttccctgt gacctgtgag acaggccctg gcagcccctc tggccacgct     360

atgggcacag ctggggtgta ctacgtgatg gtgaccagca ccctgtctat cttccagggc     420

aagatcaagc ccacctacag gttcaggtgc ctgaacgtga tcctgtggct gggcttctgg     480

gctgtgcagc tgaacgtgtg cctgagcagg atctacctgg ctgcccactt cccccaccag     540

gtggtggctg gggtgctgtc tgggattgct gtggctgaga ccttcagcca catccactct     600

atctacaacg ccagcctgaa gaagtacttc ctgatcacct tcttcctgtt cagctttgct     660

attggcttct acctgctgct gaagggcctg ggggtggacc tgctgtggac cctggagaag     720

gcccagaggt ggtgtgagca gcctgagtgg gtgcacattg acaccacccc ctttgccagc     780

ctgctgaaga acctgggcac cctgtttggc ctgggcctgg ccctgaacag ctctatgtac     840

agggagagct gcaagggcaa gctgagcaag tggctgccct tcaggctgag ctgtattgtg     900

gccagcctgg tgctgctgca cgtgtttgac agcctgaagc cccccagcca ggtggagctg     960

gtgttctacg tgctgagctt ctgcaagtct gctgtggtgc ccctggcctc tgtgtctgtg    1020

atcccctact gcctggccca ggtgctgggc cagccccaca agaagagcct gtag          1074


<210> 55
<211> 3134
<212> DNA
<213> Artificial Sequence


<220> 
<223> hAAT_HBB2 co_hG6pc mut co2 (expression cassette)

<400> 55
aaggctcaga ggcacacagg agtttctggg ctcaccctgc ccccttccaa cccctcagtt      60

cccatcctcc agcagctgtt tgtgtgctgc ctctgaagtc cacactgaac aaacttcagc     120

ctactcatgt ccctaaaatg ggcaaacatt gcaagcagca aacagcaaac acacagccct     180

ccctgcctgc tgaccttgga gctggggcag aggtcagaga cctctctggg cccatgccac     240

ctccaacatc cactcgaccc cttggaattt cggtggagag gagcagaggt tgtcctggcg     300

tggtttaggt agtgtgagag gggtacccgg ggatcttgct accagtggaa cagccactaa     360

ggattctgca gtgagagcag agggccagct aagtggtact ctcccagaga ctgtctgact     420

cacgccaccc cctccacctt ggacacagga cgctgtggtt tctgagccag gtacaatgac     480

tcctttcggt aagtgcagtg gaagctgtac actgcccagg caaagcgtcc gggcagcgta     540

ggcgggcgac tcagatccca gccagtggac ttagcccctg tttgctcctc cgataactgg     600

ggtgaccttg gttaatattc accagcagcc tcccccgttg cccctctgga tccactgctt     660

aaatacggac gaggacaggg ccctgtctcc tcagcttcag gcaccaccac tgacctggga     720

cagtgaatag atcctgagaa cttcagggtg agtctatggg acccttgatg ttttctttcc     780

ccttcttttc tatggttaag ttcatgtcat aggaagggga gaagtaacag ggtacacata     840

ttgaccaaat cagggtaatt ttgcatttgt aattttaaaa aatgctttct tcttttaata     900

tacttttttg tttatcttat ttctaatact ttccctaatc tctttctttc agggcaataa     960

tgatacaatg tatcatgcct ctttgcacca ttctaaagaa taacagtgat aatttctggg    1020

ttaaggcaat agcaatattt ctgcatataa atatttctgc atataaattg taactgatgt    1080

aagaggtttc atattgctaa tagcagctac aatccagcta ccattctgct tttattttct    1140

ggttgggata aggctggatt attctgagtc caagctaggc ccttttgcta atcttgttca    1200

tacctcttat cttcctccca cagctcctgg gcaacctgct ggtctctctg ctggcccatc    1260

actttggcaa agcacgcgtg ccaccatgga ggaggggatg aacgtgctgc acgactttgg    1320

gatccagagc acccactacc tgcaggtgaa ctaccaggac agccaggact ggtttatcct    1380

ggtgtctgtg attgctgacc tgaggaacgc cttctacgtg ctgttcccta tctggttcca    1440

cctgcaggag gctgtgggga tcaagctgct gtgggtggct gtgattgggg actggctgaa    1500

cctggtgttc aagtggatcc tgtttggcca gaggccctac tggtgggtgc tggacacaga    1560

ctactacagc aacacctctg tgcccctgat caagcagttc cctgtgacct gtgagacagg    1620

ccctggcagc ccctctggcc acgctatggg cacagctggg gtgtactacg tgatggtgac    1680

cagcaccctg tctatcttcc agggcaagat caagcccacc tacaggttca ggtgcctgaa    1740

cgtgatcctg tggctgggct tctgggctgt gcagctgaac gtgtgcctga gcaggatcta    1800

cctggctgcc cacttccccc accaggtggt ggctggggtg ctgtctggga ttgctgtggc    1860

tgagaccttc agccacatcc actctatcta caacgccagc ctgaagaagt acttcctgat    1920

caccttcttc ctgttcagct ttgctattgg cttctacctg ctgctgaagg gcctgggggt    1980

ggacctgctg tggaccctgg agaaggccca gaggtggtgt gagcagcctg agtgggtgca    2040

cattgacacc accccctttg ccagcctgct gaagaacctg ggcaccctgt ttggcctggg    2100

cctggccctg aacagctcta tgtacaggga gagctgcaag ggcaagctga gcaagtggct    2160

gcccttcagg ctgagctgta ttgtggccag cctggtgctg ctgcacgtgt ttgacagcct    2220

gaagcccccc agccaggtgg agctggtgtt ctacgtgctg agcttctgca agtctgctgt    2280

ggtgcccctg gcctctgtgt ctgtgatccc ctactgcctg gcccaggtgc tgggccagcc    2340

ccacaagaag agcctgtagc tcgaggaatt caccccacca gtgcaggctg cctatcagaa    2400

agtggtggct ggtgtggcta atgccctggc ccacaagtat cactaagctc gctttcttgc    2460

tgtccaattt ctattaaagg ttcctttgtt ccctaagtcc aactactaaa ctgggggata    2520

ttatgaaggg ccttgagcat ctggattctg cctaataaaa aacatttatt ttcattgcaa    2580

tgatgtattt aaattatttc tgaatatttt actaaaaagg gaatgtggga ggtcagtgca    2640

tttaaaacat aaagaaatga agagctagtt caaaccttgg gaaaatacac tatatcttaa    2700

actccatgaa agaaggtgag gctgcaaaca gctaatgcac attggcaaca gcccctgatg    2760

cctatgcctt attcatccct cagaaaagga ttcaagtaga ggcttgattt ggaggttaaa    2820

gttttgctat gctgtatttt acattactta ttgttttagc tgtcctcatg aatgtctttt    2880

cactacccat ttgcttatcc tgcatctctc agccttgact ccactcagtt ctcttgctta    2940

gagataccac ctttcccctg aagtgttcct tccatgtttt acggcgagat ggtttctcct    3000

cgcctggcca ctcagcctta gttgtctctg ttgtcttata gaggtctact tgaagaagga    3060

aaaacagggg gcatggtttg actgtcctgt gagcccttct tccctgcctc ccccactcac    3120

agtgacccgg aatc                                                      3134


<210> 56
<211> 3134
<212> RNA
<213> Artificial Sequence


<220> 
<223> hAAT_HBB2 co_hG6pc wt (expression cassette used in example 4 and
      5) - comprising SEQ ID NO: 2

<400> 56
aaggctcaga ggcacacagg agtttctggg ctcaccctgc ccccttccaa cccctcagtt      60

cccatcctcc agcagctgtt tgtgtgctgc ctctgaagtc cacactgaac aaacttcagc     120

ctactcatgt ccctaaaatg ggcaaacatt gcaagcagca aacagcaaac acacagccct     180

ccctgcctgc tgaccttgga gctggggcag aggtcagaga cctctctggg cccatgccac     240

ctccaacatc cactcgaccc cttggaattt cggtggagag gagcagaggt tgtcctggcg     300

tggtttaggt agtgtgagag gggtacccgg ggatcttgct accagtggaa cagccactaa     360

ggattctgca gtgagagcag agggccagct aagtggtact ctcccagaga ctgtctgact     420

cacgccaccc cctccacctt ggacacagga cgctgtggtt tctgagccag gtacaatgac     480

tcctttcggt aagtgcagtg gaagctgtac actgcccagg caaagcgtcc gggcagcgta     540

ggcgggcgac tcagatccca gccagtggac ttagcccctg tttgctcctc cgataactgg     600

ggtgaccttg gttaatattc accagcagcc tcccccgttg cccctctgga tccactgctt     660

aaatacggac gaggacaggg ccctgtctcc tcagcttcag gcaccaccac tgacctggga     720

cagtgaatag atcctgagaa cttcagggtg agtctatggg acccttgatg ttttctttcc     780

ccttcttttc tatggttaag ttcatgtcat aggaagggga gaagtaacag ggtacacata     840

ttgaccaaat cagggtaatt ttgcatttgt aattttaaaa aatgctttct tcttttaata     900

tacttttttg tttatcttat ttctaatact ttccctaatc tctttctttc agggcaataa     960

tgatacaatg tatcatgcct ctttgcacca ttctaaagaa taacagtgat aatttctggg    1020

ttaaggcaat agcaatattt ctgcatataa atatttctgc atataaattg taactgatgt    1080

aagaggtttc atattgctaa tagcagctac aatccagcta ccattctgct tttattttct    1140

ggttgggata aggctggatt attctgagtc caagctaggc ccttttgcta atcttgttca    1200

tacctcttat cttcctccca cagctcctgg gcaacctgct ggtctctctg ctggcccatc    1260

actttggcaa agcacgcgtg ccaccatgga ggaaggaatg aatgttctcc atgactttgg    1320

gatccagtca acacattacc tccaggtgaa ttaccaagac tcccaggact ggttcatctt    1380

ggtgtccgtg atcgcagacc tcaggaatgc cttctacgtc ctcttcccca tctggttcca    1440

tcttcaggaa gctgtgggca ttaaactcct ttgggtagct gtgattggag actggctcaa    1500

cctcgtcttt aagtggattc tctttggaca gcgtccatac tggtgggttt tggatactga    1560

ctactacagc aacacttccg tgcccctgat aaagcagttc cctgtaacct gtgagactgg    1620

accagggagc ccctctggcc atgccatggg cacagcaggt gtatactacg tgatggtcac    1680

atctactctt tccatctttc agggaaagat aaagccgacc tacagatttc ggtgcttgaa    1740

tgtcattttg tggttgggat tctgggctgt gcagctgaat gtctgtctgt cacgaatcta    1800

ccttgctgct cattttcctc atcaagttgt tgctggagtc ctgtcaggca ttgctgttgc    1860

agaaactttc agccacatcc acagcatcta taatgccagc ctcaagaaat attttctcat    1920

taccttcttc ctgttcagct tcgccatcgg attttatctg ctgctcaagg gactgggtgt    1980

agacctcctg tggactctgg agaaagccca gaggtggtgc gagcagccag aatgggtcca    2040

cattgacacc acaccctttg ccagcctcct caagaacctg ggcacgctct ttggcctggg    2100

gctggctctc aactccagca tgtacaggga gagctgcaag gggaaactca gcaagtggct    2160

cccattccgc ctcagctcta ttgtagcctc cctcgtcctc ctgcacgtct ttgactcctt    2220

gaaaccccca tcccaagtcg agctggtctt ctacgtcttg tccttctgca agagtgcggt    2280

agtgcccctg gcatccgtca gtgtcatccc ctactgcctc gcccaggtcc tgggccagcc    2340

gcacaagaag tcgttgtaaa gatctgaatt caccccacca gtgcaggctg cctatcagaa    2400

agtggtggct ggtgtggcta atgccctggc ccacaagtat cactaagctc gctttcttgc    2460

tgtccaattt ctattaaagg ttcctttgtt ccctaagtcc aactactaaa ctgggggata    2520

ttatgaaggg ccttgagcat ctggattctg cctaataaaa aacatttatt ttcattgcaa    2580

tgatgtattt aaattatttc tgaatatttt actaaaaagg gaatgtggga ggtcagtgca    2640

tttaaaacat aaagaaatga agagctagtt caaaccttgg gaaaatacac tatatcttaa    2700

actccatgaa agaaggtgag gctgcaaaca gctaatgcac attggcaaca gcccctgatg    2760

cctatgcctt attcatccct cagaaaagga ttcaagtaga ggcttgattt ggaggttaaa    2820

gttttgctat gctgtatttt acattactta ttgttttagc tgtcctcatg aatgtctttt    2880

cactacccat ttgcttatcc tgcatctctc agccttgact ccactcagtt ctcttgctta    2940

gagataccac ctttcccctg aagtgttcct tccatgtttt acggcgagat ggtttctcct    3000

cgcctggcca ctcagcctta gttgtctctg ttgtcttata gaggtctact tgaagaagga    3060

aaaacagggg gcatggtttg actgtcctgt gagcccttct tccctgcctc ccccactcac    3120

agtgacccgg aatc                                                      3134


<210> 57
<211> 2217
<212> DNA
<213> Artificial Sequence


<220> 
<223> Synthetic polynucleotide coding for AAVMut5

<400> 57
atggctgccg atggttatct tccagattgg ctcgaggaca acctctctga gggcattcgc      60

gagtggtggg cgctgaaacc tggagccccg aagcccaaag ccaaccagca aaagcaggac     120

gacggccggg gtctggtgct tcctggctac aagtacctcg gacccttcaa cggactcgac     180

aagggggagc ccgtcaacgc ggcggacgca gcggccctcg agcacgacaa ggcctacgac     240

cagcagctgc aggcgggtga caatccgtac ctgcggtata accacgccga cgccgagttt     300

caggagcgtc tgcaagaaga tacgtctttt gggggcaacc tcgggcgagc agtcttccag     360

gccaagaagc gggttctcga acctctcggt ctggttgagg aaggcgctaa gacggctcct     420

ggaaagaaga gaccggtaga gccatcaccc cagcgttctc cagactcctc tacgggcatc     480

ggcaagaaag gccaacagcc cgccagaaaa agactcaatt ttggtcagac tggcgactca     540

gagtcagttc cagaccctca acctctcgga gaacctccag cagcgccctc tggtgtggga     600

cctaatacaa tggctgcagg cggtggcgca ccaatggcag acaataacga aggcgccgac     660

ggagtgggta gttcctcggg aaattggcat tgcgattcca catggctggg cgacagagtc     720

atcaccacca gcacccgaac ctgggccctg cccacctaca acaaccacct ctacaagcaa     780

atctccaacg ggacatcggg aggagccacc aacgacaaca cctacttcgg ctacagcacc     840

ccctgggggt attttgactt taacagattc cactgccact tttcaccacg tgactggcag     900

cgactcatca acaacaactg gggattccgg cccaagagac tcagcttcaa gctcttcaac     960

atccaggtca aggaggtcac gcagaatgaa ggcaccaaga ccatcgccaa taacctcacc    1020

agcaccatcc aggtgtttac ggactcggag taccagctgc cgtacgttct cggctctgcc    1080

caccagggct gcctgcctcc gttcccggcg gacgtgttca tgattcccca gtacggctac    1140

ctaacactca acaacggtag tcaggccgtg ggacgctcct ccttctactg cctggaatac    1200

tttccttcgc agatgctgag aaccggcaac aacttccagt ttacttacac cttcgaggac    1260

gtgcctttcc acagcagcta cgcccacagc cagagcttgg accggctgat gaatcctctg    1320

attgaccagt acctgtacta tctgaacaag acacaatcaa atagtggaac tcttcagcag    1380

tctcggctac tgtttagtca agctggaccc accagcatgt ctcttcaagc taaaaactgg    1440

ctgcctggac cttgctaccg ccaacaacgc gtctcaacga caaccgggca aaacaacaat    1500

agcaactttg cctggactgc tgggaccaaa taccatctga atggaagaaa ttcattggct    1560

aatcctggca tcgctatggc aacacacaaa gacgacgagg agcgtttttt tcccagtaac    1620

gggatcctga tttttggcaa acaaaatgct gccagagaca atgcggatta cagcgatgtc    1680

atgctcacca gcgaggaaga aatcaaaacc actaaccctg tggctacaga ggaatacggt    1740

atcgtggcag ataacttgca gcagcaaaac acggctcctc aaattggaac tgtcaacagc    1800

cagggggcct tacccggtat ggtctggcag aaccgggacg tgtacctgca gggtcccatc    1860

tgggccaaga ttcctcacac ggacggcaac ttccacccgt ctccgctgat gggcggcttt    1920

ggcctgaaac atcctccgcc tcagatcctg atcaagaaca cgcctgtacc tgcggatcct    1980

ccgaccacct tcaaccagtc aaagctgaac tctttcatca cgcaatacag caccggacag    2040

gtcagcgtgg aaattgaatg ggagctgcag aaggaaaaca gcaagcgctg gaaccccgag    2100

atccagtaca cctccaacta ctacaaatct acaagtgtgg actttgctgt taatacagaa    2160

ggcgtgtact ctgaaccccg ccccattggc acccgttacc tcacccgtaa tctgtaa       2217


<210> 58
<211> 738
<212> PRT
<213> Artificial Sequence


<220> 
<223> AAVmut5 (amino acid)

<400> 58
Met Ala Ala Asp Gly Tyr Leu Pro Asp Trp Leu Glu Asp Asn Leu Ser 
1               5                   10                  15      
Glu Gly Ile Arg Glu Trp Trp Ala Leu Lys Pro Gly Ala Pro Lys Pro 
            20                  25                  30          
Lys Ala Asn Gln Gln Lys Gln Asp Asp Gly Arg Gly Leu Val Leu Pro 
        35                  40                  45              
Gly Tyr Lys Tyr Leu Gly Pro Phe Asn Gly Leu Asp Lys Gly Glu Pro 
    50                  55                  60                  
Val Asn Ala Ala Asp Ala Ala Ala Leu Glu His Asp Lys Ala Tyr Asp 
65                  70                  75                  80  
Gln Gln Leu Gln Ala Gly Asp Asn Pro Tyr Leu Arg Tyr Asn His Ala 
                85                  90                  95      
Asp Ala Glu Phe Gln Glu Arg Leu Gln Glu Asp Thr Ser Phe Gly Gly 
            100                 105                 110         
Asn Leu Gly Arg Ala Val Phe Gln Ala Lys Lys Arg Val Leu Glu Pro 
        115                 120                 125             
Leu Gly Leu Val Glu Glu Gly Ala Lys Thr Ala Pro Gly Lys Lys Arg 
    130                 135                 140                 
Pro Val Glu Pro Ser Pro Gln Arg Ser Pro Asp Ser Ser Thr Gly Ile 
145                 150                 155                 160 
Gly Lys Lys Gly Gln Gln Pro Ala Arg Lys Arg Leu Asn Phe Gly Gln 
                165                 170                 175     
Thr Gly Asp Ser Glu Ser Val Pro Asp Pro Gln Pro Leu Gly Glu Pro 
            180                 185                 190         
Pro Ala Ala Pro Ser Gly Val Gly Pro Asn Thr Met Ala Ala Gly Gly 
        195                 200                 205             
Gly Ala Pro Met Ala Asp Asn Asn Glu Gly Ala Asp Gly Val Gly Ser 
    210                 215                 220                 
Ser Ser Gly Asn Trp His Cys Asp Ser Thr Trp Leu Gly Asp Arg Val 
225                 230                 235                 240 
Ile Thr Thr Ser Thr Arg Thr Trp Ala Leu Pro Thr Tyr Asn Asn His 
                245                 250                 255     
Leu Tyr Lys Gln Ile Ser Asn Gly Thr Ser Gly Gly Ala Thr Asn Asp 
            260                 265                 270         
Asn Thr Tyr Phe Gly Tyr Ser Thr Pro Trp Gly Tyr Phe Asp Phe Asn 
        275                 280                 285             
Arg Phe His Cys His Phe Ser Pro Arg Asp Trp Gln Arg Leu Ile Asn 
    290                 295                 300                 
Asn Asn Trp Gly Phe Arg Pro Lys Arg Leu Ser Phe Lys Leu Phe Asn 
305                 310                 315                 320 
Ile Gln Val Lys Glu Val Thr Gln Asn Glu Gly Thr Lys Thr Ile Ala 
                325                 330                 335     
Asn Asn Leu Thr Ser Thr Ile Gln Val Phe Thr Asp Ser Glu Tyr Gln 
            340                 345                 350         
Leu Pro Tyr Val Leu Gly Ser Ala His Gln Gly Cys Leu Pro Pro Phe 
        355                 360                 365             
Pro Ala Asp Val Phe Met Ile Pro Gln Tyr Gly Tyr Leu Thr Leu Asn 
    370                 375                 380                 
Asn Gly Ser Gln Ala Val Gly Arg Ser Ser Phe Tyr Cys Leu Glu Tyr 
385                 390                 395                 400 
Phe Pro Ser Gln Met Leu Arg Thr Gly Asn Asn Phe Gln Phe Thr Tyr 
                405                 410                 415     
Thr Phe Glu Asp Val Pro Phe His Ser Ser Tyr Ala His Ser Gln Ser 
            420                 425                 430         
Leu Asp Arg Leu Met Asn Pro Leu Ile Asp Gln Tyr Leu Tyr Tyr Leu 
        435                 440                 445             
Asn Lys Thr Gln Ser Asn Ser Gly Thr Leu Gln Gln Ser Arg Leu Leu 
    450                 455                 460                 
Phe Ser Gln Ala Gly Pro Thr Ser Met Ser Leu Gln Ala Lys Asn Trp 
465                 470                 475                 480 
Leu Pro Gly Pro Cys Tyr Arg Gln Gln Arg Val Ser Thr Thr Thr Gly 
                485                 490                 495     
Gln Asn Asn Asn Ser Asn Phe Ala Trp Thr Ala Gly Thr Lys Tyr His 
            500                 505                 510         
Leu Asn Gly Arg Asn Ser Leu Ala Asn Pro Gly Ile Ala Met Ala Thr 
        515                 520                 525             
His Lys Asp Asp Glu Glu Arg Phe Phe Pro Ser Asn Gly Ile Leu Ile 
    530                 535                 540                 
Phe Gly Lys Gln Asn Ala Ala Arg Asp Asn Ala Asp Tyr Ser Asp Val 
545                 550                 555                 560 
Met Leu Thr Ser Glu Glu Glu Ile Lys Thr Thr Asn Pro Val Ala Thr 
                565                 570                 575     
Glu Glu Tyr Gly Ile Val Ala Asp Asn Leu Gln Gln Gln Asn Thr Ala 
            580                 585                 590         
Pro Gln Ile Gly Thr Val Asn Ser Gln Gly Ala Leu Pro Gly Met Val 
        595                 600                 605             
Trp Gln Asn Arg Asp Val Tyr Leu Gln Gly Pro Ile Trp Ala Lys Ile 
    610                 615                 620                 
Pro His Thr Asp Gly Asn Phe His Pro Ser Pro Leu Met Gly Gly Phe 
625                 630                 635                 640 
Gly Leu Lys His Pro Pro Pro Gln Ile Leu Ile Lys Asn Thr Pro Val 
                645                 650                 655     
Pro Ala Asp Pro Pro Thr Thr Phe Asn Gln Ser Lys Leu Asn Ser Phe 
            660                 665                 670         
Ile Thr Gln Tyr Ser Thr Gly Gln Val Ser Val Glu Ile Glu Trp Glu 
        675                 680                 685             
Leu Gln Lys Glu Asn Ser Lys Arg Trp Asn Pro Glu Ile Gln Tyr Thr 
    690                 695                 700                 
Ser Asn Tyr Tyr Lys Ser Thr Ser Val Asp Phe Ala Val Asn Thr Glu 
705                 710                 715                 720 
Gly Val Tyr Ser Glu Pro Arg Pro Ile Gly Thr Arg Tyr Leu Thr Arg 
                725                 730                 735     
Asn Leu 
        

