                         SEQUENCE LISTING

<110>  INSTITUT CURIE
       HONING BIOSCIENCES
       CNRS
       UNIVERSITY OF ZURICH
 
<120>  Single domain antibodies and their use in cancer therapies

<130>  DOI 2019 MOUTEL - BCT210076

<150>  EP20305535.5
<151>  2020-05-20

<160>  45    

<170>  PatentIn version 3.5

<210>  1
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  artificial sequence

<400>  1

Arg Thr Tyr Ser Arg Asp Thr 
1               5           


<210>  2
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  artificial sequence

<400>  2

Ser Arg His Ser His Thr Thr 
1               5           


<210>  3
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  artificial sequence

<400>  3

Glu Trp Asp Val Phe Asp Met His Tyr Ala Leu Pro Pro Met Trp 
1               5                   10                  15  


<210>  4
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  artificial sequence

<400>  4

Tyr Thr Ser Arg Ser Ser Ala 
1               5           


<210>  5
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  artificial sequence

<400>  5

Asp Leu Thr Gly Tyr Pro Tyr 
1               5           


<210>  6
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  artificial sequence

<400>  6

Ala Tyr Gln Asp Asp Lys Trp Thr Tyr Gly Ser Gln His Gly Lys 
1               5                   10                  15  


<210>  7
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  artificial sequence

<400>  7

Arg Thr Trp Leu Thr Thr 
1               5       


<210>  8
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  artificial sequence

<400>  8

Ser Phe Ser Ser Lys Gln Gly 
1               5           


<210>  9
<211>  18
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  artificial sequence

<400>  9

Tyr Ala Ser Tyr Pro Arg His Gln Gly Asn Gly Arg Trp Lys Asp Phe 
1               5                   10                  15      


Val Glu 
        


<210>  10
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  artificial sequence

<400>  10

Thr Gly Tyr Ala Leu Asp Asp 
1               5           


<210>  11
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  artificial sequence

<400>  11

Asp Asp Glu Ser Met Ala Asp 
1               5           


<210>  12
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  artificial sequence

<400>  12

Ser Tyr Lys Glu Tyr Lys Tyr Gln Ser Gly His His Tyr Phe Ala 
1               5                   10                  15  


<210>  13
<211>  26
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  artificial sequence

<400>  13

Glu Val Gln Leu Gln Ala Ser Gly Gly Gly Phe Val Gln Pro Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly 
            20                  25      


<210>  14
<211>  19
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  artificial sequence

<400>  14

Met Gly Trp Phe Arg Gln Ala Pro Gly Lys Glu Arg Glu Phe Val Ser 
1               5                   10                  15      


Ala Ile Ser 
            


<210>  15
<211>  39
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  artificial sequence

<400>  15

Tyr Tyr Ala Asp Ser Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn 
1               5                   10                  15      


Ser Lys Asn Thr Val Tyr Leu Gln Met Asn Ser Leu Arg Ala Glu Asp 
            20                  25                  30          


Thr Ala Thr Tyr Tyr Cys Ala 
        35                  


<210>  16
<211>  12
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  artificial sequence

<400>  16

Tyr Trp Gly Gln Gly Thr Gln Val Thr Val Ser Ser 
1               5                   10          


<210>  17
<211>  26
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  artificial sequence

<400>  17

Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly 
            20                  25      


<210>  18
<211>  19
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  artificial sequence

<400>  18

Met Gly Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val Ser 
1               5                   10                  15      


Ala Ile Ser 
            


<210>  19
<211>  39
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  artificial sequence

<400>  19

Tyr Tyr Ala Asp Ser Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn 
1               5                   10                  15      


Ser Lys Asn Thr Val Tyr Leu Gln Met Asn Ser Leu Arg Ala Glu Asp 
            20                  25                  30          


Thr Ala Val Tyr Tyr Cys Ala 
        35                  


<210>  20
<211>  12
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  artificial sequence

<400>  20

Tyr Arg Gly Gln Gly Thr Leu Val Thr Val Ser Ser 
1               5                   10          


<210>  21
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  artificial sequence

<400>  21
cgtacataca gtcgtgatac a                                                 21


<210>  22
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  artificial sequence

<400>  22
agtcgtcatt cgcatacaac a                                                 21


<210>  23
<211>  45
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  artificial sequence

<400>  23
gagtgggacg tttttgacat gcactatgca ctgcctccta tgtgg                       45


<210>  24
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  artificial sequence

<400>  24
tatacaagtc gttcgtccgc a                                                 21


<210>  25
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  artificial sequence

<400>  25
gacctgacag ggtatcctta c                                                 21


<210>  26
<211>  45
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  artificial sequence

<400>  26
gcatatcagg acgacaagtg gacatatggt tcgcagcacg gtaag                       45


<210>  27
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  artificial sequence

<400>  27
cgtacttcat ggctgactac a                                                 21


<210>  28
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  artificial sequence

<400>  28
agtttttcga gtaagcaggg t                                                 21


<210>  29
<211>  54
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  artificial sequence

<400>  29
tatgcatcgt atcctcgtca ccagggtaac ggtcgttgga aggactttgt tgag             54


<210>  30
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  artificial sequence

<400>  30
acaggatatg ccctcgatga t                                                 21


<210>  31
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  artificial sequence

<400>  31
gatgatgaga gtatggctga t                                                 21


<210>  32
<211>  45
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  artificial sequence

<400>  32
tcctataagg agtacaagta tcagagcgga caccactatt tcgct                       45


<210>  33
<211>  78
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  artificial sequence

<400>  33
gaagtgcagc tgcaggcttc cgggggagga tttgtgcagc cgggggggtc attgcgactg       60

agctgcgccg catccggg                                                     78


<210>  34
<211>  57
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  artificial sequence

<400>  34
atgggctggt ttcgtcaggc ccctggcaag gagagagagt tcgtttccgc catctcg          57


<210>  35
<211>  117
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  artificial sequence

<400>  35
tactacgctg acagcgtaaa gggaagattt acaattagcc gggataactc caaaaacacg       60

gtctatctcc agatgaacag cctcagggcc gaggacacag ctacgtatta ctgtgca         117


<210>  36
<211>  36
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  artificial sequence

<400>  36
tattggggac aggggacgca ggtaactgtg agtagc                                 36


<210>  37
<211>  78
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  artificial sequence

<400>  37
gaagtgcagc tggtggagtc cgggggagga ctggtgcagc cgggggggtc attgcgactg       60

agctgcgccg catccggg                                                     78


<210>  38
<211>  57
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  artificial sequence

<400>  38
atgggctggg ttcgtcaggc ccctggcaag gggctggagt gggtttccgc catctcc          57


<210>  39
<211>  117
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  artificial sequence

<400>  39
tattacgctg acagcgtaaa gggaagattt acaattagcc gggataactc caaaaacacg       60

gtctatctcc agatgaacag cctcagggcc gaggacactg cagtgtatta ctgtgca         117


<210>  40
<211>  36
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  artificial sequence

<400>  40
tatcgtggac aggggacgct ggtaactgtg agtagc                                 36


<210>  41
<211>  125
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  artificial sequence

<400>  41

Glu Val Gln Leu Gln Ala Ser Gly Gly Gly Phe Val Gln Pro Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Arg Thr Tyr Ser Arg Asp 
            20                  25                  30          


Thr Met Gly Trp Phe Arg Gln Ala Pro Gly Lys Glu Arg Glu Phe Val 
        35                  40                  45              


Ser Ala Ile Ser Ser Arg His Ser His Thr Thr Tyr Tyr Ala Asp Ser 
    50                  55                  60                  


Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr Val 
65                  70                  75                  80  


Tyr Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Thr Tyr Tyr 
                85                  90                  95      


Cys Ala Glu Trp Asp Val Phe Asp Met His Tyr Ala Leu Pro Pro Met 
            100                 105                 110         


Trp Tyr Trp Gly Gln Gly Thr Gln Val Thr Val Ser Ser 
        115                 120                 125 


<210>  42
<211>  125
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  artificial sequence

<400>  42

Glu Val Gln Leu Gln Ala Ser Gly Gly Gly Phe Val Gln Pro Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Tyr Thr Ser Arg Ser Ser 
            20                  25                  30          


Ala Met Gly Trp Phe Arg Gln Ala Pro Gly Lys Glu Arg Glu Phe Val 
        35                  40                  45              


Ser Ala Ile Ser Asp Leu Thr Gly Tyr Pro Tyr Tyr Tyr Ala Asp Ser 
    50                  55                  60                  


Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr Val 
65                  70                  75                  80  


Tyr Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Thr Tyr Tyr 
                85                  90                  95      


Cys Ala Ala Tyr Gln Asp Asp Lys Trp Thr Tyr Gly Ser Gln His Gly 
            100                 105                 110         


Lys Tyr Trp Gly Gln Gly Thr Gln Val Thr Val Ser Ser 
        115                 120                 125 


<210>  43
<211>  128
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  artificial sequence

<400>  43

Glu Val Gln Leu Gln Ala Ser Gly Gly Gly Phe Val Gln Pro Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Arg Thr Ser Trp Leu Thr 
            20                  25                  30          


Thr Met Gly Trp Phe Arg Gln Ala Pro Gly Lys Glu Arg Glu Phe Val 
        35                  40                  45              


Ser Ala Ile Ser Ser Phe Ser Ser Lys Gln Gly Tyr Tyr Ala Asp Ser 
    50                  55                  60                  


Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr Val 
65                  70                  75                  80  


Tyr Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Thr Tyr Tyr 
                85                  90                  95      


Cys Ala Tyr Ala Ser Tyr Pro Arg His Gln Gly Asn Gly Arg Trp Lys 
            100                 105                 110         


Asp Phe Val Glu Tyr Trp Gly Gln Gly Thr Gln Val Thr Val Ser Ser 
        115                 120                 125             


<210>  44
<211>  125
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  artificial sequence

<400>  44

Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Thr Gly Tyr Ala Leu Asp 
            20                  25                  30          


Asp Met Gly Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val 
        35                  40                  45              


Ser Ala Ile Ser Asp Asp Glu Ser Met Ala Asp Tyr Tyr Ala Asp Ser 
    50                  55                  60                  


Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr Val 
65                  70                  75                  80  


Tyr Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr 
                85                  90                  95      


Cys Ala Ser Tyr Lys Glu Tyr Lys Tyr Gln Ser Gly His His Tyr Phe 
            100                 105                 110         


Ala Tyr Arg Gly Gln Gly Thr Leu Val Thr Val Ser Ser 
        115                 120                 125 


<210>  45
<211>  802
<212>  PRT
<213>  Homo sapiens

<400>  45

Met Arg Leu Leu Leu Ala Leu Leu Gly Val Leu Leu Ser Val Pro Gly 
1               5                   10                  15      


Pro Pro Val Leu Ser Leu Glu Ala Ser Glu Glu Val Glu Leu Glu Pro 
            20                  25                  30          


Cys Leu Ala Pro Ser Leu Glu Gln Gln Glu Gln Glu Leu Thr Val Ala 
        35                  40                  45              


Leu Gly Gln Pro Val Arg Leu Cys Cys Gly Arg Ala Glu Arg Gly Gly 
    50                  55                  60                  


His Trp Tyr Lys Glu Gly Ser Arg Leu Ala Pro Ala Gly Arg Val Arg 
65                  70                  75                  80  


Gly Trp Arg Gly Arg Leu Glu Ile Ala Ser Phe Leu Pro Glu Asp Ala 
                85                  90                  95      


Gly Arg Tyr Leu Cys Leu Ala Arg Gly Ser Met Ile Val Leu Gln Asn 
            100                 105                 110         


Leu Thr Leu Ile Thr Gly Asp Ser Leu Thr Ser Ser Asn Asp Asp Glu 
        115                 120                 125             


Asp Pro Lys Ser His Arg Asp Pro Ser Asn Arg His Ser Tyr Pro Gln 
    130                 135                 140                 


Gln Ala Pro Tyr Trp Thr His Pro Gln Arg Met Glu Lys Lys Leu His 
145                 150                 155                 160 


Ala Val Pro Ala Gly Asn Thr Val Lys Phe Arg Cys Pro Ala Ala Gly 
                165                 170                 175     


Asn Pro Thr Pro Thr Ile Arg Trp Leu Lys Asp Gly Gln Ala Phe His 
            180                 185                 190         


Gly Glu Asn Arg Ile Gly Gly Ile Arg Leu Arg His Gln His Trp Ser 
        195                 200                 205             


Leu Val Met Glu Ser Val Val Pro Ser Asp Arg Gly Thr Tyr Thr Cys 
    210                 215                 220                 


Leu Val Glu Asn Ala Val Gly Ser Ile Arg Tyr Asn Tyr Leu Leu Asp 
225                 230                 235                 240 


Val Leu Glu Arg Ser Pro His Arg Pro Ile Leu Gln Ala Gly Leu Pro 
                245                 250                 255     


Ala Asn Thr Thr Ala Val Val Gly Ser Asp Val Glu Leu Leu Cys Lys 
            260                 265                 270         


Val Tyr Ser Asp Ala Gln Pro His Ile Gln Trp Leu Lys His Ile Val 
        275                 280                 285             


Ile Asn Gly Ser Ser Phe Gly Ala Asp Gly Phe Pro Tyr Val Gln Val 
    290                 295                 300                 


Leu Lys Thr Ala Asp Ile Asn Ser Ser Glu Val Glu Val Leu Tyr Leu 
305                 310                 315                 320 


Arg Asn Val Ser Ala Glu Asp Ala Gly Glu Tyr Thr Cys Leu Ala Gly 
                325                 330                 335     


Asn Ser Ile Gly Leu Ser Tyr Gln Ser Ala Trp Leu Thr Val Leu Pro 
            340                 345                 350         


Glu Glu Asp Pro Thr Trp Thr Ala Ala Ala Pro Glu Ala Arg Tyr Thr 
        355                 360                 365             


Asp Ile Ile Leu Tyr Ala Ser Gly Ser Leu Ala Leu Ala Val Leu Leu 
    370                 375                 380                 


Leu Leu Ala Gly Leu Tyr Arg Gly Gln Ala Leu His Gly Arg His Pro 
385                 390                 395                 400 


Arg Pro Pro Ala Thr Val Gln Lys Leu Ser Arg Phe Pro Leu Ala Arg 
                405                 410                 415     


Gln Phe Ser Leu Glu Ser Gly Ser Ser Gly Lys Ser Ser Ser Ser Leu 
            420                 425                 430         


Val Arg Gly Val Arg Leu Ser Ser Ser Gly Pro Ala Leu Leu Ala Gly 
        435                 440                 445             


Leu Val Ser Leu Asp Leu Pro Leu Asp Pro Leu Trp Glu Phe Pro Arg 
    450                 455                 460                 


Asp Arg Leu Val Leu Gly Lys Pro Leu Gly Glu Gly Cys Phe Gly Gln 
465                 470                 475                 480 


Val Val Arg Ala Glu Ala Phe Gly Met Asp Pro Ala Arg Pro Asp Gln 
                485                 490                 495     


Ala Ser Thr Val Ala Val Lys Met Leu Lys Asp Asn Ala Ser Asp Lys 
            500                 505                 510         


Asp Leu Ala Asp Leu Val Ser Glu Met Glu Val Met Lys Leu Ile Gly 
        515                 520                 525             


Arg His Lys Asn Ile Ile Asn Leu Leu Gly Val Cys Thr Gln Glu Gly 
    530                 535                 540                 


Pro Leu Tyr Val Ile Val Glu Cys Ala Ala Lys Gly Asn Leu Arg Glu 
545                 550                 555                 560 


Phe Leu Arg Ala Arg Arg Pro Pro Gly Pro Asp Leu Ser Pro Asp Gly 
                565                 570                 575     


Pro Arg Ser Ser Glu Gly Pro Leu Ser Phe Pro Val Leu Val Ser Cys 
            580                 585                 590         


Ala Tyr Gln Val Ala Arg Gly Met Gln Tyr Leu Glu Ser Arg Lys Cys 
        595                 600                 605             


Ile His Arg Asp Leu Ala Ala Arg Asn Val Leu Val Thr Glu Asp Asn 
    610                 615                 620                 


Val Met Lys Ile Ala Asp Phe Gly Leu Ala Arg Gly Val His His Ile 
625                 630                 635                 640 


Asp Tyr Tyr Lys Lys Thr Ser Asn Gly Arg Leu Pro Val Lys Trp Met 
                645                 650                 655     


Ala Pro Glu Ala Leu Phe Asp Arg Val Tyr Thr His Gln Ser Asp Val 
            660                 665                 670         


Trp Ser Phe Gly Ile Leu Leu Trp Glu Ile Phe Thr Leu Gly Gly Ser 
        675                 680                 685             


Pro Tyr Pro Gly Ile Pro Val Glu Glu Leu Phe Ser Leu Leu Arg Glu 
    690                 695                 700                 


Gly His Arg Met Asp Arg Pro Pro His Cys Pro Pro Glu Leu Tyr Gly 
705                 710                 715                 720 


Leu Met Arg Glu Cys Trp His Ala Ala Pro Ser Gln Arg Pro Thr Phe 
                725                 730                 735     


Lys Gln Leu Val Glu Ala Leu Asp Lys Val Leu Leu Ala Val Ser Glu 
            740                 745                 750         


Glu Tyr Leu Asp Leu Arg Leu Thr Phe Gly Pro Tyr Ser Pro Ser Gly 
        755                 760                 765             


Gly Asp Ala Ser Ser Thr Cys Ser Ser Ser Asp Ser Val Phe Ser His 
    770                 775                 780                 


Asp Pro Leu Pro Leu Gly Ser Ser Ser Phe Pro Phe Gly Ser Gly Val 
785                 790                 795                 800 


Gln Thr 
        


