                         SEQUENCE LISTING

<110>  Nanodigmbio (Nanjing) Biotechnology Co., Ltd
 
<120>  Universial blocking sequence and uses thereof

<130>  PN145771NAGD

<160>  13    

<170>  PatentIn version 3.5

<210>  1
<211>  72
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Universial blocking sequence


<220>
<221>  misc_feature
<222>  (21)..(30)
<223>  tag blocking sequence,n is A,T,C,or G

<400>  1
ctctcagtac gtcagcagtt nnnnnnnnnn caactccttg gctcacagaa cgacatggct       60

acgatccgac tt                                                           72


<210>  2
<211>  60
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Universial blocking sequence


<220>
<221>  misc_feature
<222>  (19)..(28)
<223>  tag blocking sequence,n is ATC or G

<400>  2
gcatggcgac cttatcagnn nnnnnnnntt gtcttcctaa gaccgcttgg cctccgactt       60


<210>  3
<211>  72
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Universial blocking sequence


<220>
<221>  misc_feature
<222>  (1)..(20)
<223>  left non-tag blocking sequencebases at 6,12,15,18 and 20 are 
       modified by LAN or BNA

<220>
<221>  misc_feature
<222>  (21)..(30)
<223>  tag blocking sequencen is I or C3 spacer

<220>
<221>  misc_feature
<222>  (31)..(72)
<223>  right non-tag blocking sequencebases at 33,39,43,50,54,59 and 
       67are modified by LAN or BNA,the end of 72 is C3 spacer

<400>  3
ctctcagtac gtcagcagtt nnnnnnnnnn caactccttg gctcacagaa cgacatggct       60

acgatccgac tt                                                           72


<210>  4
<211>  60
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Universial blocking sequence


<220>
<221>  misc_feature
<222>  (1)..(18)
<223>  left non-tag blocking sequencebases at 4,8,10,14 and 18 are 
       modified by LANorBNA

<220>
<221>  misc_feature
<222>  (1)..(18)
<223>  right non-tag blocking sequencebases at 36,40,43,47,50,53 and 58 
       are modified by LANorBNA

<220>
<221>  misc_feature
<222>  (19)..(28)
<223>  tag blocking sequencen is I or C3 spacer

<400>  4
gcatggcgac cttatcagnn nnnnnnnntt gtcttcctaa gaccgcttgg cctccgactt       60


<210>  5
<211>  72
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Universial blocking sequence


<220>
<221>  misc_feature
<222>  (1)..(20)
<223>  left non-tag blocking sequencebases at 4,7,9,12,15,18 and 20 are 
       modified by LANorBNA

<220>
<221>  misc_feature
<222>  (21)..(30)
<223>  tag blocking sequencen is I or C3 spacer

<220>
<221>  misc_feature
<222>  (31)..(72)
<223>  right non-tag blocking sequencebases at 
       33,39,43,47,50,54,57,59,63 and 67 are modified by LANorBNA,the 
       end of 72 is C3 spacer

<400>  5
ctctcagtac gtcagcagtt nnnnnnnnnn caactccttg gctcacagaa cgacatggct       60

acgatccgac tt                                                           72


<210>  6
<211>  60
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Universial blocking sequence


<220>
<221>  misc_feature
<222>  (1)..(18)
<223>  left non-tag blocking sequencebases at 4,6,8,10,12,14 and 18 are 
       modified by LANorBNA

<220>
<221>  misc_feature
<222>  (19)..(28)
<223>  tag blocking sequencen is I or C3 spacer

<220>
<221>  misc_feature
<222>  (29)..(60)
<223>  right non-tag blocking sequencebases at 
       32,36,40,43,45,47,50,53,56 and 58 are modified by LANorBNA,the 
       end of 60 is C3 spacer

<400>  6
gcatggcgac cttatcagnn nnnnnnnntt gtcttcctaa gaccgcttgg cctccgactt       60


<210>  7
<211>  60
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Universial blocking sequence


<220>
<221>  misc_feature
<222>  (1)..(18)
<223>  left non-tag blocking sequencebases at 4,8,10,14 are modified by 
       LANorBNA

<220>
<221>  misc_feature
<222>  (19)..(28)
<223>  tag blocking sequencen is I or C3 spacer

<220>
<221>  misc_feature
<222>  (29)..(60)
<223>  right non-tag blocking sequencebases at 36,40,43,47,50 and 58 are
       modified by LANorBNA,the end of 60 is C3 spacer

<400>  7
gcatggcgac cttatcagnn nnnnnnnntt gtcttcctaa gaccgcttgg cctccgactt       60


<210>  8
<211>  72
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Universial blocking sequence


<220>
<221>  misc_feature
<222>  (1)..(20)
<223>  left non-tag blocking sequencebases at 4,7,9,12,15,16,18 and 20 
       are modified by LANorBNA

<220>
<221>  misc_feature
<222>  (21)..(30)
<223>  tag blocking sequencen is I or C3 spacer

<220>
<221>  misc_feature
<222>  (31)..(72)
<223>  right non-tag blocking sequencebases at 
       33,39,43,47,50,54,57,59,63,67,70 are modified by LANorBNA,the end
       of 72 is C3 spacer

<400>  8
ctctcagtac gtcagcagtt nnnnnnnnnn caactccttg gctcacagaa cgacatggct       60

acgatccgac tt                                                           72


<210>  9
<211>  60
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Universial blocking sequence


<220>
<221>  misc_feature
<222>  (1)..(18)
<223>  left non-tag blocking sequencebases at 4,6,8,10,12,14,16 and 
       18are modified by LANorBNA

<220>
<221>  misc_feature
<222>  (19)..(28)
<223>  tag blocking sequencen is I or C3 spacer

<220>
<221>  misc_feature
<222>  (29)..(60)
<223>  right non-tag blocking sequencebases at 
       32,36,40,43,45,47,50,53,55,56 and 58 are modified by LANorBNA,the
       end of 60 is C3 spacer

<400>  9
gcatggcgac cttatcagnn nnnnnnnntt gtcttcctaa gaccgcttgg cctccgactt       60


<210>  10
<211>  72
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Universial blocking sequence


<220>
<221>  misc_feature
<222>  (1)..(20)
<223>  tag blocking sequencebases at 4,5,7,9,12,15 and 18 are modified 
       by LANorBNA

<220>
<221>  misc_feature
<222>  (21)..(30)
<223>  tag blocking sequencen is I or C3 spacer

<220>
<221>  misc_feature
<222>  (31)..(72)
<223>  right non-tag blocking sequencebases at 
       39,43,47,50,54,57,59,63,67,70 are modified by LANorBNA,the end of
       72 is C3 spacer

<400>  10
ctctcagtac gtcagcagtt nnnnnnnnnn caactccttg gctcacagaa cgacatggct       60

acgatccgac tt                                                           72


<210>  11
<211>  60
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Universial blocking sequence


<220>
<221>  misc_feature
<222>  (1)..(18)
<223>  left non-tag blocking sequencebases at 2,4,6,8,10,12 and 14 are 
       modified by LANorBNA

<220>
<221>  misc_feature
<222>  (19)..(28)
<223>  tag blocking sequencen is I or C3 spacer

<220>
<221>  misc_feature
<222>  (29)..(60)
<223>  right non-tag blocking sequencebases at 
       36,40,43,45,47,50,53,55,56 and 58 are modified by LANorBNA,the 
       end of 60 is C3 spacer

<400>  11
gcatggcgac cttatcagnn nnnnnnnntt gtcttcctaa gaccgcttgg cctccgactt       60


<210>  12
<211>  72
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Universial blocking sequence


<220>
<221>  misc_feature
<222>  (1)..(20)
<223>  tag blocking sequencebases at 7,9,12,15,16,18 and 20 are modified
       by LANorBNA

<220>
<221>  misc_feature
<222>  (21)..(30)
<223>  tag blocking sequencen is I or C3 spacer

<220>
<221>  misc_feature
<222>  (31)..(72)
<223>  right non-tag blocking sequencebases at 
       33,36,39,43,47,50,54,57,59 and 63 are modified by LANorBNA,the 
       end of 72 is C3 spacer

<400>  12
ctctcagtac gtcagcagtt nnnnnnnnnn caactccttg gctcacagaa cgacatggct       60

acgatccgac tt                                                           72


<210>  13
<211>  60
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Universial blocking sequence


<220>
<221>  misc_feature
<222>  (1)..(18)
<223>  left non-tag blocking sequencebases at 6,8,10,12,14,16 and 18 are
       modified by LANorBNA

<220>
<221>  misc_feature
<222>  (19)..(28)
<223>  tag blocking sequencen is I or C3 spacer

<220>
<221>  misc_feature
<222>  (29)..(60)
<223>  right non-tag blocking sequencebases at 
       32,36,37,40,43,45,47,50,53 and 56 are modified by LANorBNA,the 
       end of 60 is C3 spacer

<400>  13
gcatggcgac cttatcagnn nnnnnnnntt gtcttcctaa gaccgcttgg cctccgactt       60


