                         SEQUENCE LISTING

<110>  Eli Lilly and Company
 
<120>  Extended Time Action Acylated Insulin Compounds

<130>  X22664

<150>  US 63/025,463
<151>  2020-05-15

<160>  8     

<170>  PatentIn version 3.5

<210>  1
<211>  21
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct


<220>
<221>  DISULFID
<222>  (6)..(11)

<400>  1

Gly Ile Val Glu Gln Cys Cys Thr Ser Ile Cys Ser Leu Glu Gln Leu 
1               5                   10                  15      


Glu Asn Tyr Cys Asn 
            20      


<210>  2
<211>  29
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct


<220>
<221>  MOD_RES
<222>  (29)..(29)
<223>  Epsilon amino group of Lys is chemically modified by conjugation 
       with HO2C-(CH2)18-CO-gammaGlu-gammaGlu-gammaGlu-X-, wherein X is 
       -Lys-Gly-, -Lys-(2-[2-(2-aminoethoxy)ethoxy]acetic acid)-, or 
       -epsilonLys-Gly-

<400>  2

Phe Val Asn Gln His Leu Cys Gly Ser His Leu Val Glu Ala Leu His 
1               5                   10                  15      


Leu Val Cys Gly Glu Arg Gly Phe His Tyr Thr Pro Lys 
            20                  25                  


<210>  3
<211>  29
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct


<220>
<221>  MOD_RES
<222>  (29)..(29)
<223>  Epsilon amino group of Lys is chemically modified by conjugation 
       with HO2C-(CH2)18-CO-gammaGlu-gammaGlu-gammaGlu-Lys-Gly-

<400>  3

Phe Val Asn Gln His Leu Cys Gly Ser His Leu Val Glu Ala Leu His 
1               5                   10                  15      


Leu Val Cys Gly Glu Arg Gly Phe His Tyr Thr Pro Lys 
            20                  25                  


<210>  4
<211>  29
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct


<220>
<221>  MOD_RES
<222>  (29)..(29)
<223>  Epsilon amino group of Lys is chemically modified by conjugation 
       with 
       HO2C-(CH2)18-CO-gammaGlu-gammaGlu-gammaGlu-Lys-(2-[2-(2-aminoetho
       xy)ethoxy]acetic acid)-

<400>  4

Phe Val Asn Gln His Leu Cys Gly Ser His Leu Val Glu Ala Leu His 
1               5                   10                  15      


Leu Val Cys Gly Glu Arg Gly Phe His Tyr Thr Pro Lys 
            20                  25                  


<210>  5
<211>  29
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct


<220>
<221>  MOD_RES
<222>  (29)..(29)
<223>  Epsilon amino group of Lys is chemically modified by conjugation 
       with HO2C-(CH2)18-CO-gammaGlu-gammaGlu-gammaGlu-epsilonLys-Gly-

<400>  5

Phe Val Asn Gln His Leu Cys Gly Ser His Leu Val Glu Ala Leu His 
1               5                   10                  15      


Leu Val Cys Gly Glu Arg Gly Phe His Tyr Thr Pro Lys 
            20                  25                  


<210>  6
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  6

Thr Glu Thr Ser Gln Val Ala Pro Ala 
1               5                   


<210>  7
<211>  118
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct


<220>
<221>  DISULFID
<222>  (41)..(104)

<220>
<221>  DISULFID
<222>  (53)..(117)

<220>
<221>  DISULFID
<222>  (103)..(108)

<400>  7

Met His His His His His His Gln Ala Ile Phe Val Leu Gln Gly Ser 
1               5                   10                  15      


Leu Asp Gln Asp Pro Glu Phe Glu Asn Leu Tyr Phe Gln Ile Glu Gly 
            20                  25                  30          


Gly Arg Phe Val Asn Gln His Leu Cys Gly Ser His Leu Val Glu Ala 
        35                  40                  45              


Leu His Leu Val Cys Gly Glu Arg Gly Phe His Tyr Thr Pro Lys Arg 
    50                  55                  60                  


Arg Glu Ala Glu Asp Leu Gln Val Gly Gln Val Glu Leu Gly Gly Gly 
65                  70                  75                  80  


Pro Gly Ala Gly Ser Leu Gln Pro Leu Ala Leu Glu Gly Ser Leu Gln 
                85                  90                  95      


Arg Gly Ile Val Glu Gln Cys Cys Thr Ser Ile Cys Ser Leu Glu Gln 
            100                 105                 110         


Leu Glu Asn Tyr Cys Asn 
        115             


<210>  8
<211>  29
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  8

Phe Val Asn Gln His Leu Cys Gly Ser His Leu Val Glu Ala Leu His 
1               5                   10                  15      


Leu Val Cys Gly Glu Arg Gly Phe His Tyr Thr Pro Lys 
            20                  25                  


