                         SEQUENCE LISTING

<110>  Abintus Bio, Inc.
 
<120>  VECTORS AND METHODS FOR IN VIVO TRANSDUCTION

<130>  00156-002WO1

<140>  Not yet assigned
<141>  2021-05-11

<150>  US 63/023,191
<151>  2020-05-11

<150>  US 63/133,224
<151>  2020-12-31

<160>  28    

<170>  PatentIn version 3.5

<210>  1
<211>  1662
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  pBA-9b vRNA genome with multiple cloning site

<400>  1
gcgccagucc uccgauugac ugagucgccc ggguacccgu guauccaaua aacccucuug       60

caguugcauc cgacuugugg ucucgcuguu ccuugggagg gucuccucug agugauugac      120

uacccgucag cgggggucuu ucauuugggg gcucguccgg gaucgggaga ccccugccca      180

gggaccaccg acccaccacc gggagguaag cuggccagca acuuaucugu gucuguccga      240

uugucuagug ucuaugacug auuuuaugcg ccugcgucgg uacuaguuag cuaacuagcu      300

cuguaucugg cggacccgug guggaacuga cgaguucgga acacccggcc gcaacccugg      360

gagacguccc agggacuucg ggggccguuu uuguggcccg accugagucc aaaaaucccg      420

aucguuuugg acucuuuggu gcaccccccu uagaggaggg auaugugguu cugguaggag      480

acgagaaccu aaaacaguuc ccgccuccgu cugaauuuuu gcuuucgguu ugggaccgaa      540

gccgcgccgc gcgucuuguc ugcugcagca ucguucugug uugucucugu cugacugugu      600

uucuguauuu gucugagaau uaaggccaga cuguuaccac ucccugaagu uugaccuuag      660

gucacuggaa agaugucgag cggaucgcuc acaaccaguc gguagauguc aagaagagac      720

guuggguuac cuucugcucu gcagaauggc caaccuuuaa cgucggaugg ccgcgagacg      780

gcaccuuuaa ccgagaccuc aucacccagg uuaagaucaa ggucuuuuca ccuggcccgc      840

auggacaccc agaccagguc cccuacaucg ugaccuggga agccuuggcu uuugaccccc      900

cucccugggu caagcccuuu guacacccua agccuccgcc uccucuuccu ccauccgccc      960

cgucucuccc ccuugaaccu ccucguucga ccccgccucg auccucccuu uauccagccc     1020

ucacuccuuc ucuaggcgcc ggaauuaauu cucgaggggc ccagaucugc ggccgcucgc     1080

gagucgacaa gcuuggaucc aucgauaaaa uaaaagauuu uauuuagucu ccagaaaaag     1140

gggggaauga aagaccccac cuguagguuu ggcaagcuag cuuaaguaac gccauuuugc     1200

aaggcaugga aaaauacaua acugagaaua gagaaguuca gaucaagguc aggaacagau     1260

ggaacagcug aauaugggcc aaacaggaua ucugugguaa gcaguuccug ccccggcuca     1320

gggccaagaa cagauggaac agcugaauau gggccaaaca ggauaucugu gguaagcagu     1380

uccugccccg gcucagggcc aagaacagau gguccccaga ugcgguccag cccucagcag     1440

uuucuagaga accaucagau guuuccaggg ugccccaagg accugaaaug acccugugcc     1500

uuauuugaac uaaccaauca guucgcuucu cgcuucuguu cgcgcgcuuc ugcuccccga     1560

gcucaauaaa agagcccaca accccucacu cggcgcgcca guccuccgau ugacugaguc     1620

gcccggguac ccguguaucc aauaaacccu cuugcaguug ca                        1662


<210>  2
<211>  5640
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Construct 7: pBA-9b-GFPmiR 223-3p4TX

<400>  2
tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca       60

cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg      120

ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc      180

accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc      240

attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat      300

tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt      360

tttcccagtc acgacgttgt aaaacgacgg ccagtgccaa gctgactcta gaggatcgat      420

ccccggccgc tctagcttaa gtaacgccat tttgcaaggc atggaaaaat acataactga      480

gaatagagaa gttcagatca aggtcaggaa cagatggaac agctgaatat gggccaaaca      540

ggatatctgt ggtaagcagt tcctgccccg gctcagggcc aagaacagat ggaacagctg      600

aatatgggcc aaacaggata tctgtggtaa gcagttcctg ccccggctca gggccaagaa      660

cagatggtcc ccagatgcgg tccagccctc agcagtttct agagaaccat cagatgtttc      720

cagggtgccc caaggacctg aaatgaccct gtgccttatt tgaactaacc aatcagttcg      780

cttctcgctt ctgttcgcgc gcttctgctc cccgagctca ataaaagagc ccacaacccc      840

tcactcggcg cgccagtcct ccgattgact gagtcgcccg ggtacccgtg tatccaataa      900

accctcttgc agttgcatcc gacttgtggt ctcgctgttc cttgggaggg tctcctctga      960

gtgattgact acccgtcagc gggggtcttt catttggggg ctcgtccggg atcgggagac     1020

ccctgcccag ggaccaccga cccaccaccg ggaggtaagc tggccagcaa cttatctgtg     1080

tctgtccgat tgtctagtgt ctatgactga ttttatgcgc ctgcgtcggt actagttagc     1140

taactagctc tgtatctggc ggacccgtgg tggaactgac gagttcggaa cacccggccg     1200

caaccctggg agacgtccca gggacttcgg gggccgtttt tgtggcccga cctgagtcca     1260

aaaatcccga tcgttttgga ctctttggtg cacccccctt agaggaggga tatgtggttc     1320

tggtaggaga cgagaaccta aaacagttcc cgcctccgtc tgaatttttg ctttcggttt     1380

gggaccgaag ccgcgccgcg cgtcttgtct gctgcagcat cgttctgtgt tgtctctgtc     1440

tgactgtgtt tctgtatttg tctgagaatt aaggccagac tgttaccact ccctgaagtt     1500

tgaccttagg tcactggaaa gatgtcgagc ggatcgctca caaccagtcg gtagatgtca     1560

agaagagacg ttgggttacc ttctgctctg cagaatggcc aacctttaac gtcggatggc     1620

cgcgagacgg cacctttaac cgagacctca tcacccaggt taagatcaag gtcttttcac     1680

ctggcccgca tggacaccca gaccaggtcc cctacatcgt gacctgggaa gccttggctt     1740

ttgacccccc tccctgggtc aagccctttg tacaccctaa gcctccgcct cctcttcctc     1800

catccgcccc gtctctcccc cttgaacctc ctcgttcgac cccgcctcga tcctcccttt     1860

atccagccct cactccttct ctaggcgccg gaattaattc tcgagatggc cagcaagggc     1920

gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc     1980

cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa gctgaccctg     2040

aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccttg     2100

acctacggcg tgcagtgctt cgcccgctac cccgaccaca tgaagcagca cgacttcttc     2160

aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa ggacgacggc     2220

aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag     2280

ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac     2340

tacaacagcc acaaggtcta tatcaccgcc gacaagcaga agaacggcat caaggtgaac     2400

ttcaagaccc gccacaacat cgaggacggc agcgtgcagc tcgccgacca ctaccagcag     2460

aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagcacccag     2520

tccgccctga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg     2580

accgccgccg ggatcactct cggcatggac gagctgtaca agtgatgggg tatttgacaa     2640

actgacacgt atggggtatt tgacaaactg acatcgatgg ggtatttgac aaactgacat     2700

cactggggta tttgacaaac tgacagcggc cgctcgcgag tcgacaagct tggatccatc     2760

gataaaataa aagattttat ttagtctcca gaaaaagggg ggaatgaaag accccacctg     2820

taggtttggc aagctagctt aagtaacgcc attttgcaag gcatggaaaa atacataact     2880

gagaatagag aagttcagat caaggtcagg aacagatgga acagctgaat atgggccaaa     2940

caggatatct gtggtaagca gttcctgccc cggctcaggg ccaagaacag atggaacagc     3000

tgaatatggg ccaaacagga tatctgtggt aagcagttcc tgccccggct cagggccaag     3060

aacagatggt ccccagatgc ggtccagccc tcagcagttt ctagagaacc atcagatgtt     3120

tccagggtgc cccaaggacc tgaaatgacc ctgtgcctta tttgaactaa ccaatcagtt     3180

cgcttctcgc ttctgttcgc gcgcttctgc tccccgagct caataaaaga gcccacaacc     3240

cctcactcgg cgcgccagtc ctccgattga ctgagtcgcc cgggtacccg tgtatccaat     3300

aaaccctctt gcagttgcat ccgacttgtg gtctcgctgt tccttgggag ggtctcctct     3360

gagtgattga ctacccgtca gcgggggtct ttcatttgaa gccgaattcg taatcatggt     3420

catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg     3480

gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt     3540

tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg     3600

gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg     3660

actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa     3720

tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc     3780

aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc     3840

ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat     3900

aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc     3960

cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcaaagct     4020

cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg     4080

aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc     4140

cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga     4200

ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa     4260

gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta     4320

gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc     4380

agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg     4440

acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga     4500

tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg     4560

agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct     4620

gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg     4680

agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc     4740

cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa     4800

ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc     4860

cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt     4920

cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc     4980

ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt     5040

tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc     5100

catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt     5160

gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata     5220

gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga     5280

tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag     5340

catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa     5400

aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt     5460

attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga     5520

aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtctaag     5580

aaaccattat tatcatgaca ttaacctata aaaataggcg tatcacgagg ccctttcgtc     5640


<210>  3
<211>  8395
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Construct 16: pBA-9b-hCD19CAR miRTCMVpromIL12

<400>  3
tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca       60

cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg      120

ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc      180

accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc      240

attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat      300

tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt      360

tttcccagtc acgacgttgt aaaacgacgg ccagtgccaa gctgactcta gaggatcgat      420

ccccggccgc tctagcttaa gtaacgccat tttgcaaggc atggaaaaat acataactga      480

gaatagagaa gttcagatca aggtcaggaa cagatggaac agctgaatat gggccaaaca      540

ggatatctgt ggtaagcagt tcctgccccg gctcagggcc aagaacagat ggaacagctg      600

aatatgggcc aaacaggata tctgtggtaa gcagttcctg ccccggctca gggccaagaa      660

cagatggtcc ccagatgcgg tccagccctc agcagtttct agagaaccat cagatgtttc      720

cagggtgccc caaggacctg aaatgaccct gtgccttatt tgaactaacc aatcagttcg      780

cttctcgctt ctgttcgcgc gcttctgctc cccgagctca ataaaagagc ccacaacccc      840

tcactcggcg cgccagtcct ccgattgact gagtcgcccg ggtacccgtg tatccaataa      900

accctcttgc agttgcatcc gacttgtggt ctcgctgttc cttgggaggg tctcctctga      960

gtgattgact acccgtcagc gggggtcttt catttggggg ctcgtccggg atcgggagac     1020

ccctgcccag ggaccaccga cccaccaccg ggaggtaagc tggccagcaa cttatctgtg     1080

tctgtccgat tgtctagtgt ctatgactga ttttatgcgc ctgcgtcggt actagttagc     1140

taactagctc tgtatctggc ggacccgtgg tggaactgac gagttcggaa cacccggccg     1200

caaccctggg agacgtccca gggacttcgg gggccgtttt tgtggcccga cctgagtcca     1260

aaaatcccga tcgttttgga ctctttggtg cacccccctt agaggaggga tatgtggttc     1320

tggtaggaga cgagaaccta aaacagttcc cgcctccgtc tgaatttttg ctttcggttt     1380

gggaccgaag ccgcgccgcg cgtcttgtct gctgcagcat cgttctgtgt tgtctctgtc     1440

tgactgtgtt tctgtatttg tctgagaatt aaggccagac tgttaccact ccctgaagtt     1500

tgaccttagg tcactggaaa gatgtcgagc ggatcgctca caaccagtcg gtagatgtca     1560

agaagagacg ttgggttacc ttctgctctg cagaatggcc aacctttaac gtcggatggc     1620

cgcgagacgg cacctttaac cgagacctca tcacccaggt taagatcaag gtcttttcac     1680

ctggcccgca tggacaccca gaccaggtcc cctacatcgt gacctgggaa gccttggctt     1740

ttgacccccc tccctgggtc aagccctttg tacaccctaa gcctccgcct cctcttcctc     1800

catccgcccc gtctctcccc cttgaacctc ctcgttcgac cccgcctcga tcctcccttt     1860

atccagccct cactccttct ctaggcgccg gaattaattc tcgaggggcc cagatctgcg     1920

gccgcatggc cttaccagtg accgccttgc tcctgccgct ggccttgctg ctccacgccg     1980

ccaggccgga catccagatg acacagacta catcctccct gtctgcctct ctgggagaca     2040

gagtcaccat cagttgcagg gcaagtcagg acattagtaa atatttaaat tggtatcagc     2100

agaaaccaga tggaactgtt aaactcctga tctaccatac atcaagatta cactcaggag     2160

tcccatcaag gttcagtggc agtgggtctg gaacagatta ttctctcacc attagcaacc     2220

tggagcaaga agatattgcc acttactttt gccaacaggg taatacgctt ccgtacacgt     2280

tcggaggggg gaccaagctg gagatcacag gtggcggtgg ctcgggcggt ggtgggtcgg     2340

gtggcggcgg atctgaggtg aaactgcagg agtcaggacc tggcctggtg gcgccctcac     2400

agagcctgtc cgtcacatgc actgtctcag gggtctcatt acccgactat ggtgtaagct     2460

ggattcgcca gcctccacga aagggtctgg agtggctggg agtaatatgg ggtagtgaaa     2520

ccacatacta taattcagct ctcaaatcca gactgaccat catcaaggac aactccaaga     2580

gccaagtttt cttaaaaatg aacagtctgc aaactgatga cacagccatt tactactgtg     2640

ccaaacatta ttactacggt ggtagctatg ctatggacta ctggggccaa ggaacctcag     2700

tcaccgtctc ctcaaccacg acgccagcgc cgcgaccacc aacaccggcg cccaccatcg     2760

cgtcgcagcc cctgtccctg cgcccagagg cgtgccggcc agcggcgggg ggcgcagtgc     2820

acacgagggg gctggacttc gcctgtgata tctacatctg ggcgcccttg gccgggactt     2880

gtggggtcct tctcctgtca ctggttatca ccctttactg caaacggggc agaaagaaac     2940

tcctgtatat attcaaacaa ccatttatga gaccagtaca aactactcaa gaggaagatg     3000

gctgtagctg ccgatttcca gaagaagaag aaggaggatg tgaactgaga gtgaagttca     3060

gcaggagcgc agacgccccc gcgtacaagc agggccagaa ccagctctat aacgagctca     3120

atctaggacg aagagaggag tacgatgttt tggacaagag acgtggccgg gaccctgaga     3180

tggggggaaa gccgagaagg aagaaccctc aggaaggcct gtacaatgaa ctgcagaaag     3240

ataagatggc ggaggcctac agtgagattg ggatgaaagg cgagcgccgg aggggcaagg     3300

ggcacgatgg cctttaccag ggtctcagta cagccaccaa ggacacctac gacgcccttc     3360

acatgcaggc cctgccccct cgctaggtcg actggggtat ttgacaaact gacacgtatg     3420

gggtatttga caaactgaca tcgatggggt atttgacaaa ctgacatcac tggggtattt     3480

gacaaactga catgctggga cattgattat tgactagtta ttaatagtaa tcaattacgg     3540

ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg gtaaatggcc     3600

cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg tatgttccca     3660

tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta cggtaaactg     3720

cccacttggc agtacatcaa gtgtatcata tgccaagtac gccccctatt gacgtcaatg     3780

acggtaaatg gcccgcctgg cattatgccc agtacatgac cttatgggac tttcctactt     3840

ggcagtacat ctacgtatta gtcatcgcta ttaccatggt gatgcggttt tggcagtaca     3900

tcaatgggcg tggatagcgg tttgactcac ggggatttcc aagtctccac cccattgacg     3960

tcaatgggag tttgttttgg caccaaaatc aacgggactt tccaaaatgt cgtaacaact     4020

ccgccccatt gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat ataagcagag     4080

cttaaggcca ccatgtggcc ccccggcagc gccagccagc ccccccccag ccccgccgcc     4140

gccaccggcc tgcaccccgc cgccagaccc gtgagcctgc agtgcagact gagcatgtgc     4200

cccgccagaa gcctgctgct ggtggccacc ctggtgctgc tggaccacct gagcctggcc     4260

agaaacctgc ccgtggccac ccccgacccc ggcatgttcc cctgcctgca ccacagccag     4320

aacctgctga gagccgtgag caacatgctg cagaaggcca gacagaccct ggagttctac     4380

ccctgcacca gcgaggagat cgaccacgag gacatcacca aggacaagac cagcaccgtg     4440

gaggcctgcc tgcccctgga gctgaccaag aacgagagct gcctgaacag cagagagacc     4500

agcttcatca ccaacggcag ctgcctggcc agcagaaaga ccagcttcat gatggccctg     4560

tgcctgagca gcatctacga ggacctgaag atgtaccagg tggagttcaa gaccatgaac     4620

gccaagctgc tgatggaccc caagagacag atcttcctgg accagaacat gctggccgtg     4680

atcgacgagc tgatgcaggc cctgaacttc aacagcgaga ccgtgcccca gaagagcagc     4740

ctggaggagc ccgacttcta caagaccaag atcaagctgt gcatcctgct gcacgccttc     4800

agaatcagag ccgtgaccat cgacagagtg atgagctacc tgaacgccag cggcagcacc     4860

agcggcagcg gcctgcccgg cagcggcggc ggcagcaccc tgggcagaaa cctgcccgtg     4920

gccacccccg accccggcat gttcccctgc ctgcaccaca gccagaacct gctgagagcc     4980

gtgagcaaca tgctgcagaa ggccagacag accctggagt tctacccctg caccagcgag     5040

gagatcgacc acgaggacat caccaaggac aagaccagca ccgtggaggc ctgcctgccc     5100

ctggagctga ccaagaacga gagctgcctg aacagcagag agaccagctt catcaccaac     5160

ggcagctgcc tggccagcag aaagaccagc ttcatgatgg ccctgtgcct gagcagcatc     5220

tacgaggacc tgaagatgta ccaggtggag ttcaagacca tgaacgccaa gctgctgatg     5280

gaccccaaga gacagatctt cctggaccag aacatgctgg ccgtgatcga cgagctgatg     5340

caggccctga acttcaacag cgagaccgtg ccccagaaga gcagcctgga ggagcccgac     5400

ttctacaaga ccaagatcaa gctgtgcatc ctgctgcacg ccttcagaat cagagccgtg     5460

accatcgaca gagtgatgag ctacctgaac gccagcgatc aagcttggat ccatcgataa     5520

aataaaagat tttatttagt ctccagaaaa aggggggaat gaaagacccc acctgtaggt     5580

ttggcaagct agcttaagta acgccatttt gcaaggcatg gaaaaataca taactgagaa     5640

tagagaagtt cagatcaagg tcaggaacag atggaacagc tgaatatggg ccaaacagga     5700

tatctgtggt aagcagttcc tgccccggct cagggccaag aacagatgga acagctgaat     5760

atgggccaaa caggatatct gtggtaagca gttcctgccc cggctcaggg ccaagaacag     5820

atggtcccca gatgcggtcc agccctcagc agtttctaga gaaccatcag atgtttccag     5880

ggtgccccaa ggacctgaaa tgaccctgtg ccttatttga actaaccaat cagttcgctt     5940

ctcgcttctg ttcgcgcgct tctgctcccc gagctcaata aaagagccca caacccctca     6000

ctcggcgcgc cagtcctccg attgactgag tcgcccgggt acccgtgtat ccaataaacc     6060

ctcttgcagt tgcatccgac ttgtggtctc gctgttcctt gggagggtct cctctgagtg     6120

attgactacc cgtcagcggg ggtctttcat ttgaagccga attcgtaatc atggtcatag     6180

ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc     6240

ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc     6300

tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa     6360

cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg     6420

ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg     6480

ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag     6540

gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac     6600

gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga     6660

taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt     6720

accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca aagctcacgc     6780

tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc     6840

cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta     6900

agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat     6960

gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca     7020

gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct     7080

tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt     7140

acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct     7200

cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc     7260

acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa     7320

acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta     7380

tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc     7440

ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat     7500

ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta     7560

tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt     7620

aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt     7680

ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg     7740

ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc     7800

gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc     7860

gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg     7920

cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga     7980

actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta     8040

ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct     8100

tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag     8160

ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga     8220

agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat     8280

aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc     8340

attattatca tgacattaac ctataaaaat aggcgtatca cgaggccctt tcgtc          8395


<210>  4
<211>  8380
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Construct 15: pBA-9b-hCD19CAR miRTCMVpromIL7R-CPT

<400>  4
tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca       60

cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg      120

ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc      180

accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc      240

attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat      300

tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt      360

tttcccagtc acgacgttgt aaaacgacgg ccagtgccaa gctgactcta gaggatcgat      420

ccccggccgc tctagcttaa gtaacgccat tttgcaaggc atggaaaaat acataactga      480

gaatagagaa gttcagatca aggtcaggaa cagatggaac agctgaatat gggccaaaca      540

ggatatctgt ggtaagcagt tcctgccccg gctcagggcc aagaacagat ggaacagctg      600

aatatgggcc aaacaggata tctgtggtaa gcagttcctg ccccggctca gggccaagaa      660

cagatggtcc ccagatgcgg tccagccctc agcagtttct agagaaccat cagatgtttc      720

cagggtgccc caaggacctg aaatgaccct gtgccttatt tgaactaacc aatcagttcg      780

cttctcgctt ctgttcgcgc gcttctgctc cccgagctca ataaaagagc ccacaacccc      840

tcactcggcg cgccagtcct ccgattgact gagtcgcccg ggtacccgtg tatccaataa      900

accctcttgc agttgcatcc gacttgtggt ctcgctgttc cttgggaggg tctcctctga      960

gtgattgact acccgtcagc gggggtcttt catttggggg ctcgtccggg atcgggagac     1020

ccctgcccag ggaccaccga cccaccaccg ggaggtaagc tggccagcaa cttatctgtg     1080

tctgtccgat tgtctagtgt ctatgactga ttttatgcgc ctgcgtcggt actagttagc     1140

taactagctc tgtatctggc ggacccgtgg tggaactgac gagttcggaa cacccggccg     1200

caaccctggg agacgtccca gggacttcgg gggccgtttt tgtggcccga cctgagtcca     1260

aaaatcccga tcgttttgga ctctttggtg cacccccctt agaggaggga tatgtggttc     1320

tggtaggaga cgagaaccta aaacagttcc cgcctccgtc tgaatttttg ctttcggttt     1380

gggaccgaag ccgcgccgcg cgtcttgtct gctgcagcat cgttctgtgt tgtctctgtc     1440

tgactgtgtt tctgtatttg tctgagaatt aaggccagac tgttaccact ccctgaagtt     1500

tgaccttagg tcactggaaa gatgtcgagc ggatcgctca caaccagtcg gtagatgtca     1560

agaagagacg ttgggttacc ttctgctctg cagaatggcc aacctttaac gtcggatggc     1620

cgcgagacgg cacctttaac cgagacctca tcacccaggt taagatcaag gtcttttcac     1680

ctggcccgca tggacaccca gaccaggtcc cctacatcgt gacctgggaa gccttggctt     1740

ttgacccccc tccctgggtc aagccctttg tacaccctaa gcctccgcct cctcttcctc     1800

catccgcccc gtctctcccc cttgaacctc ctcgttcgac cccgcctcga tcctcccttt     1860

atccagccct cactccttct ctaggcgccg gaattaattc tcgaggggcc cagatctgcg     1920

gccgcatggc cttaccagtg accgccttgc tcctgccgct ggccttgctg ctccacgccg     1980

ccaggccgga catccagatg acacagacta catcctccct gtctgcctct ctgggagaca     2040

gagtcaccat cagttgcagg gcaagtcagg acattagtaa atatttaaat tggtatcagc     2100

agaaaccaga tggaactgtt aaactcctga tctaccatac atcaagatta cactcaggag     2160

tcccatcaag gttcagtggc agtgggtctg gaacagatta ttctctcacc attagcaacc     2220

tggagcaaga agatattgcc acttactttt gccaacaggg taatacgctt ccgtacacgt     2280

tcggaggggg gaccaagctg gagatcacag gtggcggtgg ctcgggcggt ggtgggtcgg     2340

gtggcggcgg atctgaggtg aaactgcagg agtcaggacc tggcctggtg gcgccctcac     2400

agagcctgtc cgtcacatgc actgtctcag gggtctcatt acccgactat ggtgtaagct     2460

ggattcgcca gcctccacga aagggtctgg agtggctggg agtaatatgg ggtagtgaaa     2520

ccacatacta taattcagct ctcaaatcca gactgaccat catcaaggac aactccaaga     2580

gccaagtttt cttaaaaatg aacagtctgc aaactgatga cacagccatt tactactgtg     2640

ccaaacatta ttactacggt ggtagctatg ctatggacta ctggggccaa ggaacctcag     2700

tcaccgtctc ctcaaccacg acgccagcgc cgcgaccacc aacaccggcg cccaccatcg     2760

cgtcgcagcc cctgtccctg cgcccagagg cgtgccggcc agcggcgggg ggcgcagtgc     2820

acacgagggg gctggacttc gcctgtgata tctacatctg ggcgcccttg gccgggactt     2880

gtggggtcct tctcctgtca ctggttatca ccctttactg caaacggggc agaaagaaac     2940

tcctgtatat attcaaacaa ccatttatga gaccagtaca aactactcaa gaggaagatg     3000

gctgtagctg ccgatttcca gaagaagaag aaggaggatg tgaactgaga gtgaagttca     3060

gcaggagcgc agacgccccc gcgtacaagc agggccagaa ccagctctat aacgagctca     3120

atctaggacg aagagaggag tacgatgttt tggacaagag acgtggccgg gaccctgaga     3180

tggggggaaa gccgagaagg aagaaccctc aggaaggcct gtacaatgaa ctgcagaaag     3240

ataagatggc ggaggcctac agtgagattg ggatgaaagg cgagcgccgg aggggcaagg     3300

ggcacgatgg cctttaccag ggtctcagta cagccaccaa ggacacctac gacgcccttc     3360

acatgcaggc cctgccccct cgctaggtcg actggggtat ttgacaaact gacacgtatg     3420

gggtatttga caaactgaca tcgatggggt atttgacaaa ctgacatcac tggggtattt     3480

gacaaactga cagtcctgtg cgacattgat tattgactag ttattaatag taatcaatta     3540

cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg     3600

gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc     3660

ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa     3720

ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca     3780

atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta     3840

cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt     3900

acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg     3960

acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca     4020

actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca     4080

gagcttaagg ccaccatgac catcctgggc accaccttcg gcatggtgtt cagcctgctg     4140

caggtggtga gcggcgagag cggctacgcc cagaacggcg acctggagga cgccgagctg     4200

gacgactaca gcttcagctg ctacagccag ctggaggtga acggcagcca gcacagcctg     4260

acctgcgcct tcgaggaccc cgacgtgaac atcaccaacc tggagttcga gatctgcggc     4320

gccctggtgg aggtgaagtg cctgaacttc agaaagctgc aggagatcta cttcatcgag     4380

accaagaagt tcctgctgat cggcaagagc aacatctgcg tgaaggtggg cgagaagagc     4440

ctgacctgca agaagatcga cctgaccacc atcgtgaagc ccgaggcccc cttcgacctg     4500

agcgtggtgt acagagaggg cgccaacgac ttcgtggtga ccttcaacac cagccacctg     4560

cagaagaagt acgtgaaggt gctgatgcac gacgtggcct acagacagga gaaggacgag     4620

aacaagtgga cccacgtgaa cctgagcagc accaagctga ccctgctgca gagaaagctg     4680

cagcccgccg ccatgtacga gatcaaggtg agaagcatcc ccgaccacta cttcaagggc     4740

ttctggagcg agtggagccc cagctactac ttcagaaccc ccgagatcaa caacagcagc     4800

ggcgagatgg accccatcct gctgacctgc cccaccatca gcatcctgag cttcttcagc     4860

gtggccctgc tggtgatcct ggcctgcgtg ctgtggaaga agagaatcaa gcccatcgtg     4920

tggcccagcc tgcccgacca caagaagacc ctggagcacc tgtgcaagaa gcccagaaag     4980

aacctgaacg tgagcttcaa ccccgagagc ttcctggact gccagatcca cagagtggac     5040

gacatccagg ccagagacga ggtggagggc ttcctgcagg acaccttccc ccagcagctg     5100

gaggagagcg agaagcagag actgggcggc gacgtgcaga gccccaactg ccccagcgag     5160

gacgtggtga tcacccccga gagcttcggc agagacagca gcctgacctg cctggccggc     5220

aacgtgagcg cctgcgacgc ccccatcctg agcagcagca gaagcctgga ctgcagagag     5280

agcggcaaga acggccccca cgtgtaccag gacctgctgc tgagcctggg caccaccaac     5340

agcaccctgc cccccccctt cagcctgcag agcggcatcc tgaccctgaa ccccgtggcc     5400

cagggccagc ccatcctgac cagcctgggc agcaaccagg aggaggccta cgtgaccatg     5460

agcagcttct accagaacca ggatcaagct tggatccatc gataaaataa aagattttat     5520

ttagtctcca gaaaaagggg ggaatgaaag accccacctg taggtttggc aagctagctt     5580

aagtaacgcc attttgcaag gcatggaaaa atacataact gagaatagag aagttcagat     5640

caaggtcagg aacagatgga acagctgaat atgggccaaa caggatatct gtggtaagca     5700

gttcctgccc cggctcaggg ccaagaacag atggaacagc tgaatatggg ccaaacagga     5760

tatctgtggt aagcagttcc tgccccggct cagggccaag aacagatggt ccccagatgc     5820

ggtccagccc tcagcagttt ctagagaacc atcagatgtt tccagggtgc cccaaggacc     5880

tgaaatgacc ctgtgcctta tttgaactaa ccaatcagtt cgcttctcgc ttctgttcgc     5940

gcgcttctgc tccccgagct caataaaaga gcccacaacc cctcactcgg cgcgccagtc     6000

ctccgattga ctgagtcgcc cgggtacccg tgtatccaat aaaccctctt gcagttgcat     6060

ccgacttgtg gtctcgctgt tccttgggag ggtctcctct gagtgattga ctacccgtca     6120

gcgggggtct ttcatttgaa gccgaattcg taatcatggt catagctgtt tcctgtgtga     6180

aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc     6240

tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc     6300

cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc     6360

ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt     6420

cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca     6480

ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa     6540

aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat     6600

cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc     6660

cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc     6720

gcctttctcc cttcgggaag cgtggcgctt tctcaaagct cacgctgtag gtatctcagt     6780

tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac     6840

cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg     6900

ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca     6960

gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc     7020

gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa     7080

accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa     7140

ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac     7200

tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta     7260

aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt     7320

taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata     7380

gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc     7440

agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac     7500

cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag     7560

tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac     7620

gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc     7680

agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg     7740

gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc     7800

atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct     7860

gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc     7920

tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc     7980

atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc     8040

agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc     8100

gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca     8160

cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt     8220

tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt     8280

ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat tatcatgaca     8340

ttaacctata aaaataggcg tatcacgagg ccctttcgtc                           8380


<210>  5
<211>  7456
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Construct 14: pBA-9b-hCD19CAR miRTCMVpromIL2wt

<400>  5
tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca       60

cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg      120

ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc      180

accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc      240

attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat      300

tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt      360

tttcccagtc acgacgttgt aaaacgacgg ccagtgccaa gctgactcta gaggatcgat      420

ccccggccgc tctagcttaa gtaacgccat tttgcaaggc atggaaaaat acataactga      480

gaatagagaa gttcagatca aggtcaggaa cagatggaac agctgaatat gggccaaaca      540

ggatatctgt ggtaagcagt tcctgccccg gctcagggcc aagaacagat ggaacagctg      600

aatatgggcc aaacaggata tctgtggtaa gcagttcctg ccccggctca gggccaagaa      660

cagatggtcc ccagatgcgg tccagccctc agcagtttct agagaaccat cagatgtttc      720

cagggtgccc caaggacctg aaatgaccct gtgccttatt tgaactaacc aatcagttcg      780

cttctcgctt ctgttcgcgc gcttctgctc cccgagctca ataaaagagc ccacaacccc      840

tcactcggcg cgccagtcct ccgattgact gagtcgcccg ggtacccgtg tatccaataa      900

accctcttgc agttgcatcc gacttgtggt ctcgctgttc cttgggaggg tctcctctga      960

gtgattgact acccgtcagc gggggtcttt catttggggg ctcgtccggg atcgggagac     1020

ccctgcccag ggaccaccga cccaccaccg ggaggtaagc tggccagcaa cttatctgtg     1080

tctgtccgat tgtctagtgt ctatgactga ttttatgcgc ctgcgtcggt actagttagc     1140

taactagctc tgtatctggc ggacccgtgg tggaactgac gagttcggaa cacccggccg     1200

caaccctggg agacgtccca gggacttcgg gggccgtttt tgtggcccga cctgagtcca     1260

aaaatcccga tcgttttgga ctctttggtg cacccccctt agaggaggga tatgtggttc     1320

tggtaggaga cgagaaccta aaacagttcc cgcctccgtc tgaatttttg ctttcggttt     1380

gggaccgaag ccgcgccgcg cgtcttgtct gctgcagcat cgttctgtgt tgtctctgtc     1440

tgactgtgtt tctgtatttg tctgagaatt aaggccagac tgttaccact ccctgaagtt     1500

tgaccttagg tcactggaaa gatgtcgagc ggatcgctca caaccagtcg gtagatgtca     1560

agaagagacg ttgggttacc ttctgctctg cagaatggcc aacctttaac gtcggatggc     1620

cgcgagacgg cacctttaac cgagacctca tcacccaggt taagatcaag gtcttttcac     1680

ctggcccgca tggacaccca gaccaggtcc cctacatcgt gacctgggaa gccttggctt     1740

ttgacccccc tccctgggtc aagccctttg tacaccctaa gcctccgcct cctcttcctc     1800

catccgcccc gtctctcccc cttgaacctc ctcgttcgac cccgcctcga tcctcccttt     1860

atccagccct cactccttct ctaggcgccg gaattaattc tcgaggggcc cagatctgcg     1920

gccgcatggc cttaccagtg accgccttgc tcctgccgct ggccttgctg ctccacgccg     1980

ccaggccgga catccagatg acacagacta catcctccct gtctgcctct ctgggagaca     2040

gagtcaccat cagttgcagg gcaagtcagg acattagtaa atatttaaat tggtatcagc     2100

agaaaccaga tggaactgtt aaactcctga tctaccatac atcaagatta cactcaggag     2160

tcccatcaag gttcagtggc agtgggtctg gaacagatta ttctctcacc attagcaacc     2220

tggagcaaga agatattgcc acttactttt gccaacaggg taatacgctt ccgtacacgt     2280

tcggaggggg gaccaagctg gagatcacag gtggcggtgg ctcgggcggt ggtgggtcgg     2340

gtggcggcgg atctgaggtg aaactgcagg agtcaggacc tggcctggtg gcgccctcac     2400

agagcctgtc cgtcacatgc actgtctcag gggtctcatt acccgactat ggtgtaagct     2460

ggattcgcca gcctccacga aagggtctgg agtggctggg agtaatatgg ggtagtgaaa     2520

ccacatacta taattcagct ctcaaatcca gactgaccat catcaaggac aactccaaga     2580

gccaagtttt cttaaaaatg aacagtctgc aaactgatga cacagccatt tactactgtg     2640

ccaaacatta ttactacggt ggtagctatg ctatggacta ctggggccaa ggaacctcag     2700

tcaccgtctc ctcaaccacg acgccagcgc cgcgaccacc aacaccggcg cccaccatcg     2760

cgtcgcagcc cctgtccctg cgcccagagg cgtgccggcc agcggcgggg ggcgcagtgc     2820

acacgagggg gctggacttc gcctgtgata tctacatctg ggcgcccttg gccgggactt     2880

gtggggtcct tctcctgtca ctggttatca ccctttactg caaacggggc agaaagaaac     2940

tcctgtatat attcaaacaa ccatttatga gaccagtaca aactactcaa gaggaagatg     3000

gctgtagctg ccgatttcca gaagaagaag aaggaggatg tgaactgaga gtgaagttca     3060

gcaggagcgc agacgccccc gcgtacaagc agggccagaa ccagctctat aacgagctca     3120

atctaggacg aagagaggag tacgatgttt tggacaagag acgtggccgg gaccctgaga     3180

tggggggaaa gccgagaagg aagaaccctc aggaaggcct gtacaatgaa ctgcagaaag     3240

ataagatggc ggaggcctac agtgagattg ggatgaaagg cgagcgccgg aggggcaagg     3300

ggcacgatgg cctttaccag ggtctcagta cagccaccaa ggacacctac gacgcccttc     3360

acatgcaggc cctgccccct cgctaggtcg actggggtat ttgacaaact gacacgtatg     3420

gggtatttga caaactgaca tcgatggggt atttgacaaa ctgacatcac tggggtattt     3480

gacaaactga cagtcgaccg tctggacatt gattattgac tagttattaa tagtaatcaa     3540

ttacggggtc attagttcat agcccatata tggagttccg cgttacataa cttacggtaa     3600

atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg     3660

ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt     3720

aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg     3780

tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc     3840

ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc     3900

agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca     3960

ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta     4020

acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa     4080

gcagagctta aggccaccat gtacagaatg cagctgctga gctgcatcgc cctgagcctg     4140

gccctggtga ccaactctgc ccccaccagc agcagcacca agaagaccca gctgcagctg     4200

gagcacctgc tgctggacct gcagatgatc ctgaacggca tcaacaacta caagaacccc     4260

aagctgacca gaatgctgac cttcaagttc tacatgccca agaaggccac cgagctgaag     4320

cacctgcagt gcctggagga ggagctgaag cccctggagg aggtgctgaa cctggcccag     4380

agcaagaact tccacctgag acccagagac ctgatcagca acatcaacgt gatcgtgctg     4440

gagctgaagg gcagcgagac caccttcatg tgcgagtacg ccgacgagac cgccaccatc     4500

gtggagttcc tgaacagatg gatcaccttc tgccagagca tcatcagcac cctgaccgat     4560

caagcttgga tccatcgata aaataaaaga ttttatttag tctccagaaa aaggggggaa     4620

tgaaagaccc cacctgtagg tttggcaagc tagcttaagt aacgccattt tgcaaggcat     4680

ggaaaaatac ataactgaga atagagaagt tcagatcaag gtcaggaaca gatggaacag     4740

ctgaatatgg gccaaacagg atatctgtgg taagcagttc ctgccccggc tcagggccaa     4800

gaacagatgg aacagctgaa tatgggccaa acaggatatc tgtggtaagc agttcctgcc     4860

ccggctcagg gccaagaaca gatggtcccc agatgcggtc cagccctcag cagtttctag     4920

agaaccatca gatgtttcca gggtgcccca aggacctgaa atgaccctgt gccttatttg     4980

aactaaccaa tcagttcgct tctcgcttct gttcgcgcgc ttctgctccc cgagctcaat     5040

aaaagagccc acaacccctc actcggcgcg ccagtcctcc gattgactga gtcgcccggg     5100

tacccgtgta tccaataaac cctcttgcag ttgcatccga cttgtggtct cgctgttcct     5160

tgggagggtc tcctctgagt gattgactac ccgtcagcgg gggtctttca tttgaagccg     5220

aattcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca     5280

cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa     5340

ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag     5400

ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc     5460

gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct     5520

cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg     5580

tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc     5640

cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga     5700

aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct     5760

cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg     5820

gcgctttctc aaagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag     5880

ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat     5940

cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac     6000

aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac     6060

tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc     6120

ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt     6180

tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc     6240

ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg     6300

agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca     6360

atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca     6420

cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag     6480

ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac     6540

ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc     6600

agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct     6660

agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc     6720

gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg     6780

cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc     6840

gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat     6900

tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag     6960

tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat     7020

aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg     7080

cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca     7140

cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga     7200

aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc     7260

ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata     7320

tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg     7380

ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc     7440

acgaggccct ttcgtc                                                     7456


<210>  6
<211>  7560
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Construct 13: pBA-9b-hCD19CAR miRTCMVpromIL2F42A

<400>  6
tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca       60

cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg      120

ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc      180

accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc      240

attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat      300

tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt      360

tttcccagtc acgacgttgt aaaacgacgg ccagtgccaa gctgactcta gaggatcgat      420

ccccggccgc tctagcttaa gtaacgccat tttgcaaggc atggaaaaat acataactga      480

gaatagagaa gttcagatca aggtcaggaa cagatggaac agctgaatat gggccaaaca      540

ggatatctgt ggtaagcagt tcctgccccg gctcagggcc aagaacagat ggaacagctg      600

aatatgggcc aaacaggata tctgtggtaa gcagttcctg ccccggctca gggccaagaa      660

cagatggtcc ccagatgcgg tccagccctc agcagtttct agagaaccat cagatgtttc      720

cagggtgccc caaggacctg aaatgaccct gtgccttatt tgaactaacc aatcagttcg      780

cttctcgctt ctgttcgcgc gcttctgctc cccgagctca ataaaagagc ccacaacccc      840

tcactcggcg cgccagtcct ccgattgact gagtcgcccg ggtacccgtg tatccaataa      900

accctcttgc agttgcatcc gacttgtggt ctcgctgttc cttgggaggg tctcctctga      960

gtgattgact acccgtcagc gggggtcttt catttggggg ctcgtccggg atcgggagac     1020

ccctgcccag ggaccaccga cccaccaccg ggaggtaagc tggccagcaa cttatctgtg     1080

tctgtccgat tgtctagtgt ctatgactga ttttatgcgc ctgcgtcggt actagttagc     1140

taactagctc tgtatctggc ggacccgtgg tggaactgac gagttcggaa cacccggccg     1200

caaccctggg agacgtccca gggacttcgg gggccgtttt tgtggcccga cctgagtcca     1260

aaaatcccga tcgttttgga ctctttggtg cacccccctt agaggaggga tatgtggttc     1320

tggtaggaga cgagaaccta aaacagttcc cgcctccgtc tgaatttttg ctttcggttt     1380

gggaccgaag ccgcgccgcg cgtcttgtct gctgcagcat cgttctgtgt tgtctctgtc     1440

tgactgtgtt tctgtatttg tctgagaatt aaggccagac tgttaccact ccctgaagtt     1500

tgaccttagg tcactggaaa gatgtcgagc ggatcgctca caaccagtcg gtagatgtca     1560

agaagagacg ttgggttacc ttctgctctg cagaatggcc aacctttaac gtcggatggc     1620

cgcgagacgg cacctttaac cgagacctca tcacccaggt taagatcaag gtcttttcac     1680

ctggcccgca tggacaccca gaccaggtcc cctacatcgt gacctgggaa gccttggctt     1740

ttgacccccc tccctgggtc aagccctttg tacaccctaa gcctccgcct cctcttcctc     1800

catccgcccc gtctctcccc cttgaacctc ctcgttcgac cccgcctcga tcctcccttt     1860

atccagccct cactccttct ctaggcgccg gaattaattc tcgaggggcc cagatctgcg     1920

gccgcatggc cttaccagtg accgccttgc tcctgccgct ggccttgctg ctccacgccg     1980

ccaggccgga catccagatg acacagacta catcctccct gtctgcctct ctgggagaca     2040

gagtcaccat cagttgcagg gcaagtcagg acattagtaa atatttaaat tggtatcagc     2100

agaaaccaga tggaactgtt aaactcctga tctaccatac atcaagatta cactcaggag     2160

tcccatcaag gttcagtggc agtgggtctg gaacagatta ttctctcacc attagcaacc     2220

tggagcaaga agatattgcc acttactttt gccaacaggg taatacgctt ccgtacacgt     2280

tcggaggggg gaccaagctg gagatcacag gtggcggtgg ctcgggcggt ggtgggtcgg     2340

gtggcggcgg atctgaggtg aaactgcagg agtcaggacc tggcctggtg gcgccctcac     2400

agagcctgtc cgtcacatgc actgtctcag gggtctcatt acccgactat ggtgtaagct     2460

ggattcgcca gcctccacga aagggtctgg agtggctggg agtaatatgg ggtagtgaaa     2520

ccacatacta taattcagct ctcaaatcca gactgaccat catcaaggac aactccaaga     2580

gccaagtttt cttaaaaatg aacagtctgc aaactgatga cacagccatt tactactgtg     2640

ccaaacatta ttactacggt ggtagctatg ctatggacta ctggggccaa ggaacctcag     2700

tcaccgtctc ctcaaccacg acgccagcgc cgcgaccacc aacaccggcg cccaccatcg     2760

cgtcgcagcc cctgtccctg cgcccagagg cgtgccggcc agcggcgggg ggcgcagtgc     2820

acacgagggg gctggacttc gcctgtgata tctacatctg ggcgcccttg gccgggactt     2880

gtggggtcct tctcctgtca ctggttatca ccctttactg caaacggggc agaaagaaac     2940

tcctgtatat attcaaacaa ccatttatga gaccagtaca aactactcaa gaggaagatg     3000

gctgtagctg ccgatttcca gaagaagaag aaggaggatg tgaactgaga gtgaagttca     3060

gcaggagcgc agacgccccc gcgtacaagc agggccagaa ccagctctat aacgagctca     3120

atctaggacg aagagaggag tacgatgttt tggacaagag acgtggccgg gaccctgaga     3180

tggggggaaa gccgagaagg aagaaccctc aggaaggcct gtacaatgaa ctgcagaaag     3240

ataagatggc ggaggcctac agtgagattg ggatgaaagg cgagcgccgg aggggcaagg     3300

ggcacgatgg cctttaccag ggtctcagta cagccaccaa ggacacctac gacgcccttc     3360

acatgcaggc cctgccccct cgctaggtcg acgcggtccc gcggcgcccc gcctcgatgc     3420

ggtcccgcgg cgccccgcct gcatgcggtc ccgcggcgcc ccgcctctac gcggtcccgc     3480

ggcgccccgc ctattatggg gtatttgaca aactgacacg tatggggtat ttgacaaact     3540

gacatcgatg gggtatttga caaactgaca tcactggggt atttgacaaa ctgacagtcg     3600

accgtctgga cattgattat tgactagtta ttaatagtaa tcaattacgg ggtcattagt     3660

tcatagccca tatatggagt tccgcgttac ataacttacg gtaaatggcc cgcctggctg     3720

accgcccaac gacccccgcc cattgacgtc aataatgacg tatgttccca tagtaacgcc     3780

aatagggact ttccattgac gtcaatgggt ggagtattta cggtaaactg cccacttggc     3840

agtacatcaa gtgtatcata tgccaagtac gccccctatt gacgtcaatg acggtaaatg     3900

gcccgcctgg cattatgccc agtacatgac cttatgggac tttcctactt ggcagtacat     3960

ctacgtatta gtcatcgcta ttaccatggt gatgcggttt tggcagtaca tcaatgggcg     4020

tggatagcgg tttgactcac ggggatttcc aagtctccac cccattgacg tcaatgggag     4080

tttgttttgg caccaaaatc aacgggactt tccaaaatgt cgtaacaact ccgccccatt     4140

gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat ataagcagag cttaaggcca     4200

ccatgtacag aatgcagctg ctgagctgca tcgccctgag cctggccctg gtgaccaact     4260

ctgcccccac cagcagcagc accaagaaga cccagctgca gctggagcac ctgctgctgg     4320

acctgcagat gatcctgaac ggcatcaaca actacaagaa ccccaagctg accagaatgc     4380

tgaccgccaa gttctacatg cccaagaagg ccaccgagct gaagcacctg cagtgcctgg     4440

aggaggagct gaagcccctg gaggaggtgc tgaacctggc ccagagcaag aacttccact     4500

tcgaccccag agacgtggtg agcaacatca acgtgttcgt gctggagctg aagggcagcg     4560

agaccacctt catgtgcgag tacgccgacg agaccgccac catcgtggag ttcctgaaca     4620

gatggatcac cttctgccag agcatcatca gcaccctgac cgatcaagct tggatccatc     4680

gataaaataa aagattttat ttagtctcca gaaaaagggg ggaatgaaag accccacctg     4740

taggtttggc aagctagctt aagtaacgcc attttgcaag gcatggaaaa atacataact     4800

gagaatagag aagttcagat caaggtcagg aacagatgga acagctgaat atgggccaaa     4860

caggatatct gtggtaagca gttcctgccc cggctcaggg ccaagaacag atggaacagc     4920

tgaatatggg ccaaacagga tatctgtggt aagcagttcc tgccccggct cagggccaag     4980

aacagatggt ccccagatgc ggtccagccc tcagcagttt ctagagaacc atcagatgtt     5040

tccagggtgc cccaaggacc tgaaatgacc ctgtgcctta tttgaactaa ccaatcagtt     5100

cgcttctcgc ttctgttcgc gcgcttctgc tccccgagct caataaaaga gcccacaacc     5160

cctcactcgg cgcgccagtc ctccgattga ctgagtcgcc cgggtacccg tgtatccaat     5220

aaaccctctt gcagttgcat ccgacttgtg gtctcgctgt tccttgggag ggtctcctct     5280

gagtgattga ctacccgtca gcgggggtct ttcatttgaa gccgaattcg taatcatggt     5340

catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg     5400

gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt     5460

tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg     5520

gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg     5580

actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa     5640

tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc     5700

aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc     5760

ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat     5820

aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc     5880

cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcaaagct     5940

cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg     6000

aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc     6060

cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga     6120

ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa     6180

gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta     6240

gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc     6300

agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg     6360

acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga     6420

tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg     6480

agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct     6540

gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg     6600

agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc     6660

cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa     6720

ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc     6780

cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt     6840

cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc     6900

ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt     6960

tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc     7020

catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt     7080

gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata     7140

gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga     7200

tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag     7260

catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa     7320

aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt     7380

attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga     7440

aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtctaag     7500

aaaccattat tatcatgaca ttaacctata aaaataggcg tatcacgagg ccctttcgtc     7560


<210>  7
<211>  7984
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Construct 12: pBA-9b-hCD19CAR miRTCMVprom-cJun

<400>  7
tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca       60

cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg      120

ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc      180

accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc      240

attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat      300

tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt      360

tttcccagtc acgacgttgt aaaacgacgg ccagtgccaa gctgactcta gaggatcgat      420

ccccggccgc tctagcttaa gtaacgccat tttgcaaggc atggaaaaat acataactga      480

gaatagagaa gttcagatca aggtcaggaa cagatggaac agctgaatat gggccaaaca      540

ggatatctgt ggtaagcagt tcctgccccg gctcagggcc aagaacagat ggaacagctg      600

aatatgggcc aaacaggata tctgtggtaa gcagttcctg ccccggctca gggccaagaa      660

cagatggtcc ccagatgcgg tccagccctc agcagtttct agagaaccat cagatgtttc      720

cagggtgccc caaggacctg aaatgaccct gtgccttatt tgaactaacc aatcagttcg      780

cttctcgctt ctgttcgcgc gcttctgctc cccgagctca ataaaagagc ccacaacccc      840

tcactcggcg cgccagtcct ccgattgact gagtcgcccg ggtacccgtg tatccaataa      900

accctcttgc agttgcatcc gacttgtggt ctcgctgttc cttgggaggg tctcctctga      960

gtgattgact acccgtcagc gggggtcttt catttggggg ctcgtccggg atcgggagac     1020

ccctgcccag ggaccaccga cccaccaccg ggaggtaagc tggccagcaa cttatctgtg     1080

tctgtccgat tgtctagtgt ctatgactga ttttatgcgc ctgcgtcggt actagttagc     1140

taactagctc tgtatctggc ggacccgtgg tggaactgac gagttcggaa cacccggccg     1200

caaccctggg agacgtccca gggacttcgg gggccgtttt tgtggcccga cctgagtcca     1260

aaaatcccga tcgttttgga ctctttggtg cacccccctt agaggaggga tatgtggttc     1320

tggtaggaga cgagaaccta aaacagttcc cgcctccgtc tgaatttttg ctttcggttt     1380

gggaccgaag ccgcgccgcg cgtcttgtct gctgcagcat cgttctgtgt tgtctctgtc     1440

tgactgtgtt tctgtatttg tctgagaatt aaggccagac tgttaccact ccctgaagtt     1500

tgaccttagg tcactggaaa gatgtcgagc ggatcgctca caaccagtcg gtagatgtca     1560

agaagagacg ttgggttacc ttctgctctg cagaatggcc aacctttaac gtcggatggc     1620

cgcgagacgg cacctttaac cgagacctca tcacccaggt taagatcaag gtcttttcac     1680

ctggcccgca tggacaccca gaccaggtcc cctacatcgt gacctgggaa gccttggctt     1740

ttgacccccc tccctgggtc aagccctttg tacaccctaa gcctccgcct cctcttcctc     1800

catccgcccc gtctctcccc cttgaacctc ctcgttcgac cccgcctcga tcctcccttt     1860

atccagccct cactccttct ctaggcgccg gaattaattc tcgaggggcc cagatctgcg     1920

gccgcatggc cttaccagtg accgccttgc tcctgccgct ggccttgctg ctccacgccg     1980

ccaggccgga catccagatg acacagacta catcctccct gtctgcctct ctgggagaca     2040

gagtcaccat cagttgcagg gcaagtcagg acattagtaa atatttaaat tggtatcagc     2100

agaaaccaga tggaactgtt aaactcctga tctaccatac atcaagatta cactcaggag     2160

tcccatcaag gttcagtggc agtgggtctg gaacagatta ttctctcacc attagcaacc     2220

tggagcaaga agatattgcc acttactttt gccaacaggg taatacgctt ccgtacacgt     2280

tcggaggggg gaccaagctg gagatcacag gtggcggtgg ctcgggcggt ggtgggtcgg     2340

gtggcggcgg atctgaggtg aaactgcagg agtcaggacc tggcctggtg gcgccctcac     2400

agagcctgtc cgtcacatgc actgtctcag gggtctcatt acccgactat ggtgtaagct     2460

ggattcgcca gcctccacga aagggtctgg agtggctggg agtaatatgg ggtagtgaaa     2520

ccacatacta taattcagct ctcaaatcca gactgaccat catcaaggac aactccaaga     2580

gccaagtttt cttaaaaatg aacagtctgc aaactgatga cacagccatt tactactgtg     2640

ccaaacatta ttactacggt ggtagctatg ctatggacta ctggggccaa ggaacctcag     2700

tcaccgtctc ctcaaccacg acgccagcgc cgcgaccacc aacaccggcg cccaccatcg     2760

cgtcgcagcc cctgtccctg cgcccagagg cgtgccggcc agcggcgggg ggcgcagtgc     2820

acacgagggg gctggacttc gcctgtgata tctacatctg ggcgcccttg gccgggactt     2880

gtggggtcct tctcctgtca ctggttatca ccctttactg caaacggggc agaaagaaac     2940

tcctgtatat attcaaacaa ccatttatga gaccagtaca aactactcaa gaggaagatg     3000

gctgtagctg ccgatttcca gaagaagaag aaggaggatg tgaactgaga gtgaagttca     3060

gcaggagcgc agacgccccc gcgtacaagc agggccagaa ccagctctat aacgagctca     3120

atctaggacg aagagaggag tacgatgttt tggacaagag acgtggccgg gaccctgaga     3180

tggggggaaa gccgagaagg aagaaccctc aggaaggcct gtacaatgaa ctgcagaaag     3240

ataagatggc ggaggcctac agtgagattg ggatgaaagg cgagcgccgg aggggcaagg     3300

ggcacgatgg cctttaccag ggtctcagta cagccaccaa ggacacctac gacgcccttc     3360

acatgcaggc cctgccccct cgctaggtcg actggggtat ttgacaaact gacacgtatg     3420

gggtatttga caaactgaca tcgatggggt atttgacaaa ctgacatcac tggggtattt     3480

gacaaactga catgcgctga cattgattat tgactagtta ttaatagtaa tcaattacgg     3540

ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg gtaaatggcc     3600

cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg tatgttccca     3660

tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta cggtaaactg     3720

cccacttggc agtacatcaa gtgtatcata tgccaagtac gccccctatt gacgtcaatg     3780

acggtaaatg gcccgcctgg cattatgccc agtacatgac cttatgggac tttcctactt     3840

ggcagtacat ctacgtatta gtcatcgcta ttaccatggt gatgcggttt tggcagtaca     3900

tcaatgggcg tggatagcgg tttgactcac ggggatttcc aagtctccac cccattgacg     3960

tcaatgggag tttgttttgg caccaaaatc aacgggactt tccaaaatgt cgtaacaact     4020

ccgccccatt gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat ataagcagag     4080

ctggccacca tgactgcaaa gatggaaacg accttctatg acgatgccct caacgcctcg     4140

ttcctcccgt ccgagagcgg accttatggc tacagtaacc ccaagatcct gaaacagagc     4200

atgaccctga acctggccga cccagtgggg agcctgaagc cgcacctccg cgccaagaac     4260

tcggacctcc tcacctcgcc cgacgtgggg ctgctcaagc tggcgtcgcc cgagctggag     4320

cgcctgataa tccagtccag caacgggcac atcaccacca cgccgacccc cacccagttc     4380

ctgtgcccca agaacgtgac agatgagcag gagggcttcg ccgagggctt cgtgcgcgcc     4440

ctggccgaac tgcacagcca gaacacgctg cccagcgtca cgtcggcggc gcagccggtc     4500

aacggggcag gcatggtggc tcccgcggta gcctcggtgg cagggggcag cggcagcggc     4560

ggcttcagcg ccagcctgca cagcgagccg ccggtctacg caaacctcag caacttcaac     4620

ccaggcgcgc tgagcagcgg cggcggggcg ccctcctacg gcgcggccgg cctggccttt     4680

cccgcgcaac cccagcagca gcagcagccg ccgcaccacc tgccccagca gatgcccgtg     4740

cagcacccgc ggctgcaggc cctgaaggag gagcctcaga cagtgcccga gatgcccggc     4800

gagacaccgc ccctgtcccc catcgacatg gagtcccagg agcggatcaa ggcggagagg     4860

aagcgcatga ggaaccgcat cgctgcctcc aagtgccgaa aaaggaagct ggagagaatc     4920

gcccggctgg aggaaaaagt gaaaaccttg aaagctcaga actcggagct ggcgtccacg     4980

gccaacatgc tcagggaaca ggtggcacag cttaaacaga aagtcatgaa ccacgttaac     5040

agtgggtgcc aactcatgct aacgcagcag ttgcaaacat tttgagatca agcttggatc     5100

catcgataaa ataaaagatt ttatttagtc tccagaaaaa ggggggaatg aaagacccca     5160

cctgtaggtt tggcaagcta gcttaagtaa cgccattttg caaggcatgg aaaaatacat     5220

aactgagaat agagaagttc agatcaaggt caggaacaga tggaacagct gaatatgggc     5280

caaacaggat atctgtggta agcagttcct gccccggctc agggccaaga acagatggaa     5340

cagctgaata tgggccaaac aggatatctg tggtaagcag ttcctgcccc ggctcagggc     5400

caagaacaga tggtccccag atgcggtcca gccctcagca gtttctagag aaccatcaga     5460

tgtttccagg gtgccccaag gacctgaaat gaccctgtgc cttatttgaa ctaaccaatc     5520

agttcgcttc tcgcttctgt tcgcgcgctt ctgctccccg agctcaataa aagagcccac     5580

aacccctcac tcggcgcgcc agtcctccga ttgactgagt cgcccgggta cccgtgtatc     5640

caataaaccc tcttgcagtt gcatccgact tgtggtctcg ctgttccttg ggagggtctc     5700

ctctgagtga ttgactaccc gtcagcgggg gtctttcatt tgaagccgaa ttcgtaatca     5760

tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga     5820

gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt     5880

gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga     5940

atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc     6000

actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg     6060

gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc     6120

cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc     6180

ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga     6240

ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc     6300

ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcaa     6360

agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg     6420

cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc     6480

aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga     6540

gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact     6600

agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt     6660

ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag     6720

cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg     6780

tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa     6840

aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata     6900

tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg     6960

atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata     7020

cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg     7080

gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct     7140

gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt     7200

tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc     7260

tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga     7320

tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt     7380

aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc     7440

atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa     7500

tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca     7560

catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca     7620

aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct     7680

tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc     7740

gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa     7800

tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt     7860

tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc     7920

taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcccttt     7980

cgtc                                                                  7984


<210>  8
<211>  7672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Construct 10: pBA-9b-hCD19CAR-IRES-TKmiRT+CMVprom-IL15

<400>  8
tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca       60

cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg      120

ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc      180

accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc      240

attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat      300

tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt      360

tttcccagtc acgacgttgt aaaacgacgg ccagtgccaa gctgactcta gaggatcgat      420

ccccggccgc tctagcttaa gtaacgccat tttgcaaggc atggaaaaat acataactga      480

gaatagagaa gttcagatca aggtcaggaa cagatggaac agctgaatat gggccaaaca      540

ggatatctgt ggtaagcagt tcctgccccg gctcagggcc aagaacagat ggaacagctg      600

aatatgggcc aaacaggata tctgtggtaa gcagttcctg ccccggctca gggccaagaa      660

cagatggtcc ccagatgcgg tccagccctc agcagtttct agagaaccat cagatgtttc      720

cagggtgccc caaggacctg aaatgaccct gtgccttatt tgaactaacc aatcagttcg      780

cttctcgctt ctgttcgcgc gcttctgctc cccgagctca ataaaagagc ccacaacccc      840

tcactcggcg cgccagtcct ccgattgact gagtcgcccg ggtacccgtg tatccaataa      900

accctcttgc agttgcatcc gacttgtggt ctcgctgttc cttgggaggg tctcctctga      960

gtgattgact acccgtcagc gggggtcttt catttggggg ctcgtccggg atcgggagac     1020

ccctgcccag ggaccaccga cccaccaccg ggaggtaagc tggccagcaa cttatctgtg     1080

tctgtccgat tgtctagtgt ctatgactga ttttatgcgc ctgcgtcggt actagttagc     1140

taactagctc tgtatctggc ggacccgtgg tggaactgac gagttcggaa cacccggccg     1200

caaccctggg agacgtccca gggacttcgg gggccgtttt tgtggcccga cctgagtcca     1260

aaaatcccga tcgttttgga ctctttggtg cacccccctt agaggaggga tatgtggttc     1320

tggtaggaga cgagaaccta aaacagttcc cgcctccgtc tgaatttttg ctttcggttt     1380

gggaccgaag ccgcgccgcg cgtcttgtct gctgcagcat cgttctgtgt tgtctctgtc     1440

tgactgtgtt tctgtatttg tctgagaatt aaggccagac tgttaccact ccctgaagtt     1500

tgaccttagg tcactggaaa gatgtcgagc ggatcgctca caaccagtcg gtagatgtca     1560

agaagagacg ttgggttacc ttctgctctg cagaatggcc aacctttaac gtcggatggc     1620

cgcgagacgg cacctttaac cgagacctca tcacccaggt taagatcaag gtcttttcac     1680

ctggcccgca tggacaccca gaccaggtcc cctacatcgt gacctgggaa gccttggctt     1740

ttgacccccc tccctgggtc aagccctttg tacaccctaa gcctccgcct cctcttcctc     1800

catccgcccc gtctctcccc cttgaacctc ctcgttcgac cccgcctcga tcctcccttt     1860

atccagccct cactccttct ctaggcgccg gaattaattc tcgaggggcc cagatctgcg     1920

gccgcatggc cttaccagtg accgccttgc tcctgccgct ggccttgctg ctccacgccg     1980

ccaggccgga catccagatg acacagacta catcctccct gtctgcctct ctgggagaca     2040

gagtcaccat cagttgcagg gcaagtcagg acattagtaa atatttaaat tggtatcagc     2100

agaaaccaga tggaactgtt aaactcctga tctaccatac atcaagatta cactcaggag     2160

tcccatcaag gttcagtggc agtgggtctg gaacagatta ttctctcacc attagcaacc     2220

tggagcaaga agatattgcc acttactttt gccaacaggg taatacgctt ccgtacacgt     2280

tcggaggggg gaccaagctg gagatcacag gtggcggtgg ctcgggcggt ggtgggtcgg     2340

gtggcggcgg atctgaggtg aaactgcagg agtcaggacc tggcctggtg gcgccctcac     2400

agagcctgtc cgtcacatgc actgtctcag gggtctcatt acccgactat ggtgtaagct     2460

ggattcgcca gcctccacga aagggtctgg agtggctggg agtaatatgg ggtagtgaaa     2520

ccacatacta taattcagct ctcaaatcca gactgaccat catcaaggac aactccaaga     2580

gccaagtttt cttaaaaatg aacagtctgc aaactgatga cacagccatt tactactgtg     2640

ccaaacatta ttactacggt ggtagctatg ctatggacta ctggggccaa ggaacctcag     2700

tcaccgtctc ctcaaccacg acgccagcgc cgcgaccacc aacaccggcg cccaccatcg     2760

cgtcgcagcc cctgtccctg cgcccagagg cgtgccggcc agcggcgggg ggcgcagtgc     2820

acacgagggg gctggacttc gcctgtgata tctacatctg ggcgcccttg gccgggactt     2880

gtggggtcct tctcctgtca ctggttatca ccctttactg caaacggggc agaaagaaac     2940

tcctgtatat attcaaacaa ccatttatga gaccagtaca aactactcaa gaggaagatg     3000

gctgtagctg ccgatttcca gaagaagaag aaggaggatg tgaactgaga gtgaagttca     3060

gcaggagcgc agacgccccc gcgtacaagc agggccagaa ccagctctat aacgagctca     3120

atctaggacg aagagaggag tacgatgttt tggacaagag acgtggccgg gaccctgaga     3180

tggggggaaa gccgagaagg aagaaccctc aggaaggcct gtacaatgaa ctgcagaaag     3240

ataagatggc ggaggcctac agtgagattg ggatgaaagg cgagcgccgg aggggcaagg     3300

ggcacgatgg cctttaccag ggtctcagta cagccaccaa ggacacctac gacgcccttc     3360

acatgcaggc cctgccccct cgctaggtcg actgcgcggt cccgcggcgc cccgcctcga     3420

tgcggtcccg cggcgccccg cctgcatgcg gtcccgcggc gccccgcctc tacgcggtcc     3480

cgcggcgccc cgcctattat ggggtatttg acaaactgac acgtatgggg tatttgacaa     3540

actgacatcg atggggtatt tgacaaactg acatcactgg ggtatttgac aaactgacag     3600

tcgacaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt     3660

tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg     3720

tatcttatca tgtctggatc tgcgctgaca ttgattattg actagttatt aatagtaatc     3780

aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt     3840

aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta     3900

tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg     3960

gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga     4020

cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt     4080

tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg     4140

gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc     4200

cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg     4260

taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat     4320

aagcagagct gccaccatgg cccccagaag agccagaggc tgcagaaccc tgggcctgcc     4380

cgccctgctg ctgctgctgc tgctgagacc ccccgccacc agaggcaact gggtgaacgt     4440

gatcagcgac ctgaagaaga tcgaggacct gatccagagc atgcacatcg acgccaccct     4500

gtacaccgag agcgacgtgc accccagctg caaggtgacc gccatgaagt gcttcctgct     4560

ggagctgcag gtgatcagcc tggagagcgg cgacgccagc atccacgaca ccgtggagga     4620

cctgatcatc ctggccaaca acagcctgag cagcaacggc aacgtgaccg agagcggctg     4680

caaggagtgc gaggagctgg aggagaagaa catcaaggag ttcctgcaga gcttcgtgca     4740

catcgtgcag atgttcatca acaccagcta agactagaag cttggatcca tcgataaaat     4800

aaaagatttt atttagtctc cagaaaaagg ggggaatgaa agaccccacc tgtaggtttg     4860

gcaagctagc ttaagtaacg ccattttgca aggcatggaa aaatacataa ctgagaatag     4920

agaagttcag atcaaggtca ggaacagatg gaacagctga atatgggcca aacaggatat     4980

ctgtggtaag cagttcctgc cccggctcag ggccaagaac agatggaaca gctgaatatg     5040

ggccaaacag gatatctgtg gtaagcagtt cctgccccgg ctcagggcca agaacagatg     5100

gtccccagat gcggtccagc cctcagcagt ttctagagaa ccatcagatg tttccagggt     5160

gccccaagga cctgaaatga ccctgtgcct tatttgaact aaccaatcag ttcgcttctc     5220

gcttctgttc gcgcgcttct gctccccgag ctcaataaaa gagcccacaa cccctcactc     5280

ggcgcgccag tcctccgatt gactgagtcg cccgggtacc cgtgtatcca ataaaccctc     5340

ttgcagttgc atccgacttg tggtctcgct gttccttggg agggtctcct ctgagtgatt     5400

gactacccgt cagcgggggt ctttcatttg aagccgaatt cgtaatcatg gtcatagctg     5460

tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata     5520

aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca     5580

ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc     5640

gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg     5700

cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta     5760

tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc     5820

aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag     5880

catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac     5940

caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc     6000

ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcaaag ctcacgctgt     6060

aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc     6120

gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga     6180

cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta     6240

ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta     6300

tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga     6360

tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg     6420

cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag     6480

tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc     6540

tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact     6600

tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt     6660

cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta     6720

ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta     6780

tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc     6840

gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat     6900

agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt     6960

atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg     7020

tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca     7080

gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta     7140

agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg     7200

cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact     7260

ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg     7320

ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt     7380

actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga     7440

ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc     7500

atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa     7560

caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcta agaaaccatt     7620

attatcatga cattaaccta taaaaatagg cgtatcacga ggccctttcg tc             7672


<210>  9
<211>  8424
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Construct 19: pBA-9b-hCD19CAR-IRES-TKmiRT+U6-PD1shRNA

<400>  9
tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca       60

cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg      120

ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc      180

accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc      240

attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat      300

tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt      360

tttcccagtc acgacgttgt aaaacgacgg ccagtgccaa gctgactcta gaggatcgat      420

ccccggccgc tctagcttaa gtaacgccat tttgcaaggc atggaaaaat acataactga      480

gaatagagaa gttcagatca aggtcaggaa cagatggaac agctgaatat gggccaaaca      540

ggatatctgt ggtaagcagt tcctgccccg gctcagggcc aagaacagat ggaacagctg      600

aatatgggcc aaacaggata tctgtggtaa gcagttcctg ccccggctca gggccaagaa      660

cagatggtcc ccagatgcgg tccagccctc agcagtttct agagaaccat cagatgtttc      720

cagggtgccc caaggacctg aaatgaccct gtgccttatt tgaactaacc aatcagttcg      780

cttctcgctt ctgttcgcgc gcttctgctc cccgagctca ataaaagagc ccacaacccc      840

tcactcggcg cgccagtcct ccgattgact gagtcgcccg ggtacccgtg tatccaataa      900

accctcttgc agttgcatcc gacttgtggt ctcgctgttc cttgggaggg tctcctctga      960

gtgattgact acccgtcagc gggggtcttt catttggggg ctcgtccggg atcgggagac     1020

ccctgcccag ggaccaccga cccaccaccg ggaggtaagc tggccagcaa cttatctgtg     1080

tctgtccgat tgtctagtgt ctatgactga ttttatgcgc ctgcgtcggt actagttagc     1140

taactagctc tgtatctggc ggacccgtgg tggaactgac gagttcggaa cacccggccg     1200

caaccctggg agacgtccca gggacttcgg gggccgtttt tgtggcccga cctgagtcca     1260

aaaatcccga tcgttttgga ctctttggtg cacccccctt agaggaggga tatgtggttc     1320

tggtaggaga cgagaaccta aaacagttcc cgcctccgtc tgaatttttg ctttcggttt     1380

gggaccgaag ccgcgccgcg cgtcttgtct gctgcagcat cgttctgtgt tgtctctgtc     1440

tgactgtgtt tctgtatttg tctgagaatt aaggccagac tgttaccact ccctgaagtt     1500

tgaccttagg tcactggaaa gatgtcgagc ggatcgctca caaccagtcg gtagatgtca     1560

agaagagacg ttgggttacc ttctgctctg cagaatggcc aacctttaac gtcggatggc     1620

cgcgagacgg cacctttaac cgagacctca tcacccaggt taagatcaag gtcttttcac     1680

ctggcccgca tggacaccca gaccaggtcc cctacatcgt gacctgggaa gccttggctt     1740

ttgacccccc tccctgggtc aagccctttg tacaccctaa gcctccgcct cctcttcctc     1800

catccgcccc gtctctcccc cttgaacctc ctcgttcgac cccgcctcga tcctcccttt     1860

atccagccct cactccttct ctaggcgccg gaattaattc tcgaggggcc cagatctgcg     1920

gccgcatggc cttaccagtg accgccttgc tcctgccgct ggccttgctg ctccacgccg     1980

ccaggccgga catccagatg acacagacta catcctccct gtctgcctct ctgggagaca     2040

gagtcaccat cagttgcagg gcaagtcagg acattagtaa atatttaaat tggtatcagc     2100

agaaaccaga tggaactgtt aaactcctga tctaccatac atcaagatta cactcaggag     2160

tcccatcaag gttcagtggc agtgggtctg gaacagatta ttctctcacc attagcaacc     2220

tggagcaaga agatattgcc acttactttt gccaacaggg taatacgctt ccgtacacgt     2280

tcggaggggg gaccaagctg gagatcacag gtggcggtgg ctcgggcggt ggtgggtcgg     2340

gtggcggcgg atctgaggtg aaactgcagg agtcaggacc tggcctggtg gcgccctcac     2400

agagcctgtc cgtcacatgc actgtctcag gggtctcatt acccgactat ggtgtaagct     2460

ggattcgcca gcctccacga aagggtctgg agtggctggg agtaatatgg ggtagtgaaa     2520

ccacatacta taattcagct ctcaaatcca gactgaccat catcaaggac aactccaaga     2580

gccaagtttt cttaaaaatg aacagtctgc aaactgatga cacagccatt tactactgtg     2640

ccaaacatta ttactacggt ggtagctatg ctatggacta ctggggccaa ggaacctcag     2700

tcaccgtctc ctcaaccacg acgccagcgc cgcgaccacc aacaccggcg cccaccatcg     2760

cgtcgcagcc cctgtccctg cgcccagagg cgtgccggcc agcggcgggg ggcgcagtgc     2820

acacgagggg gctggacttc gcctgtgata tctacatctg ggcgcccttg gccgggactt     2880

gtggggtcct tctcctgtca ctggttatca ccctttactg caaacggggc agaaagaaac     2940

tcctgtatat attcaaacaa ccatttatga gaccagtaca aactactcaa gaggaagatg     3000

gctgtagctg ccgatttcca gaagaagaag aaggaggatg tgaactgaga gtgaagttca     3060

gcaggagcgc agacgccccc gcgtacaagc agggccagaa ccagctctat aacgagctca     3120

atctaggacg aagagaggag tacgatgttt tggacaagag acgtggccgg gaccctgaga     3180

tggggggaaa gccgagaagg aagaaccctc aggaaggcct gtacaatgaa ctgcagaaag     3240

ataagatggc ggaggcctac agtgagattg ggatgaaagg cgagcgccgg aggggcaagg     3300

ggcacgatgg cctttaccag ggtctcagta cagccaccaa ggacacctac gacgcccttc     3360

acatgcaggc cctgccccct cgctaggtcg acacgcgtta ctggccgaag ccgcttggaa     3420

taaggccggt gtgcgtttgt ctatatgtta ttttccacca tattgccgtc ttttggcaat     3480

gtgagggccc ggaaacctgg ccctgtcttc ttgacgagca ttcctagggg tctttcccct     3540

ctcgccaaag gaatgcaagg tctgttgaat gtcgtgaagg aagcagttcc tctggaagct     3600

tcttgaagac aaacaacgtc tgtagcgacc ctttgcaggc agcggaaccc cccacctggc     3660

gacaggtgcc tctgcggcca aaagccacgt gtataagata cacctgcaaa ggcggcacaa     3720

ccccagtgcc acgttgtgag ttggatagtt gtggaaagag tcaaatggct ctcctcaagc     3780

gtattcaaca aggggctgaa ggatgcccag aaggtacccc attgtatggg atctgatctg     3840

gggcctcggt gcacatgctt tacatgtgtt tagtcgaggt taaaaaacgt ctaggccccc     3900

cgaaccacgg ggacgtggtt ttcctttgaa aaacacgatt ataaatggct tcatatcctt     3960

gccaccaaca tgcttccgct ttcgaccaag ccgcacggtc taggggccac aataatcgcc     4020

ggactgccct gcggcctcgg agacagcaga aggcaaccga agtcaggctc gagcaaaaga     4080

tgccaaccct cctgcgggtc tatatcgatg gaccccatgg aatggggaag accactacca     4140

cacaactcct ggtggcactc ggtagccggg acgacatcgt ctacgtgccc gaacccatga     4200

cttactggcg ggttctcggt gcttccgaga caatcgccaa tatctacaca acccaacacc     4260

gcctcgatca aggagaaatt agcgcagggg acgctgccgt ggtgatgaca tcagcccaaa     4320

tcaccatggg aatgccctac gccgtcaccg atgctgtcct ggcaccacac attggcggag     4380

aggccgggtc aagtcatgca ccaccaccag ccctgactat ctttctcgac cggcatccaa     4440

ttgcattcat gctgtgctat cctgccgcac gctacctgat gggaagtatg acaccacagg     4500

ccgtcctcgc cttcgttgct ctgatccctc caaccctgcc tggcactaac atcgttctcg     4560

gcgcactccc cgaagacaga cacattgatc ggctggccaa gaggcaacgg cctggcgaga     4620

gactcgatct ggctatgctg gctgctatta ggagagtgta cgggctgctg gccaatactg     4680

tgagatacct ccaaggggga ggaagctggc gcgaggattg gggccaactg tctggcgctg     4740

ctgtgccacc tcaaggcgcc gagccacagt caaatgctgg tcctaggccc cacatcggcg     4800

atactctctt tacactgttc cgggcaccag agctgctcgc acctaatgga gatctgtaca     4860

atgttttcgc ttgggccctc gatgtcctgg ctaagcggct ccggcctatg cacgtgttca     4920

tcctcgacta cgaccagagc ccagctggtt gtcgggatgc tctcctgcaa ctgaccagcg     4980

ggatggtgca gacacacgtt actactcccg gctccatccc cactatctgt gacctcgccc     5040

ggacatttgc ccgggaaatg ggcgaagcca actgatcgtt ggggtatttg acaaactgac     5100

acgtatgggg tatttgacaa actgacatcg atggggtatt tgacaaactg acatcactgg     5160

ggtatttgac aaactgacag tcgacgatca cgcgtaaggt cgggcaggaa gagggcctat     5220

ttcccatgat tccttcatat ttgcatatac gatacaaggc tgttagagag ataattagaa     5280

ttaatttgac tgtaaacaca aagatattag tacaaaatac gtgacgtaga aagtaataat     5340

ttcttgggta gtttgcagtt tttaaaatta tgttttaaaa tggactatca tatgcttacc     5400

gtaacttgaa agtatttcga tttcttggct ttatatatct tgtggaaagg acagatctgc     5460

tattatatta taattaatac ctgacccata ttaattataa tataatagct ttttggtacc     5520

gaaacaccga agcttggatc catcgataaa ataaaagatt ttatttagtc tccagaaaaa     5580

ggggggaatg aaagacccca cctgtaggtt tggcaagcta gcttaagtaa cgccattttg     5640

caaggcatgg aaaaatacat aactgagaat agagaagttc agatcaaggt caggaacaga     5700

tggaacagct gaatatgggc caaacaggat atctgtggta agcagttcct gccccggctc     5760

agggccaaga acagatggaa cagctgaata tgggccaaac aggatatctg tggtaagcag     5820

ttcctgcccc ggctcagggc caagaacaga tggtccccag atgcggtcca gccctcagca     5880

gtttctagag aaccatcaga tgtttccagg gtgccccaag gacctgaaat gaccctgtgc     5940

cttatttgaa ctaaccaatc agttcgcttc tcgcttctgt tcgcgcgctt ctgctccccg     6000

agctcaataa aagagcccac aacccctcac tcggcgcgcc agtcctccga ttgactgagt     6060

cgcccgggta cccgtgtatc caataaaccc tcttgcagtt gcatccgact tgtggtctcg     6120

ctgttccttg ggagggtctc ctctgagtga ttgactaccc gtcagcgggg gtctttcatt     6180

tgaagccgaa ttcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca     6240

caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag     6300

tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt     6360

cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc     6420

gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg     6480

tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa     6540

agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg     6600

cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga     6660

ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg     6720

tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg     6780

gaagcgtggc gctttctcaa agctcacgct gtaggtatct cagttcggtg taggtcgttc     6840

gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg     6900

gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca     6960

ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt     7020

ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag     7080

ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg     7140

gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc     7200

ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt     7260

tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt     7320

ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca     7380

gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg     7440

tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac     7500

cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg     7560

ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc     7620

gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta     7680

caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac     7740

gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc     7800

ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac     7860

tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact     7920

caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa     7980

tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt     8040

cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca     8100

ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa     8160

aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac     8220

tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg     8280

gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc     8340

gaaaagtgcc acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata     8400

ggcgtatcac gaggcccttt cgtc                                            8424


<210>  10
<211>  8420
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Construct 48: pBA-9b-hCD19CAR-IRES-TKmiRT+U6-PBCP1shRNA

<400>  10
tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca       60

cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg      120

ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc      180

accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc      240

attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat      300

tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt      360

tttcccagtc acgacgttgt aaaacgacgg ccagtgccaa gctgactcta gaggatcgat      420

ccccggccgc tctagcttaa gtaacgccat tttgcaaggc atggaaaaat acataactga      480

gaatagagaa gttcagatca aggtcaggaa cagatggaac agctgaatat gggccaaaca      540

ggatatctgt ggtaagcagt tcctgccccg gctcagggcc aagaacagat ggaacagctg      600

aatatgggcc aaacaggata tctgtggtaa gcagttcctg ccccggctca gggccaagaa      660

cagatggtcc ccagatgcgg tccagccctc agcagtttct agagaaccat cagatgtttc      720

cagggtgccc caaggacctg aaatgaccct gtgccttatt tgaactaacc aatcagttcg      780

cttctcgctt ctgttcgcgc gcttctgctc cccgagctca ataaaagagc ccacaacccc      840

tcactcggcg cgccagtcct ccgattgact gagtcgcccg ggtacccgtg tatccaataa      900

accctcttgc agttgcatcc gacttgtggt ctcgctgttc cttgggaggg tctcctctga      960

gtgattgact acccgtcagc gggggtcttt catttggggg ctcgtccggg atcgggagac     1020

ccctgcccag ggaccaccga cccaccaccg ggaggtaagc tggccagcaa cttatctgtg     1080

tctgtccgat tgtctagtgt ctatgactga ttttatgcgc ctgcgtcggt actagttagc     1140

taactagctc tgtatctggc ggacccgtgg tggaactgac gagttcggaa cacccggccg     1200

caaccctggg agacgtccca gggacttcgg gggccgtttt tgtggcccga cctgagtcca     1260

aaaatcccga tcgttttgga ctctttggtg cacccccctt agaggaggga tatgtggttc     1320

tggtaggaga cgagaaccta aaacagttcc cgcctccgtc tgaatttttg ctttcggttt     1380

gggaccgaag ccgcgccgcg cgtcttgtct gctgcagcat cgttctgtgt tgtctctgtc     1440

tgactgtgtt tctgtatttg tctgagaatt aaggccagac tgttaccact ccctgaagtt     1500

tgaccttagg tcactggaaa gatgtcgagc ggatcgctca caaccagtcg gtagatgtca     1560

agaagagacg ttgggttacc ttctgctctg cagaatggcc aacctttaac gtcggatggc     1620

cgcgagacgg cacctttaac cgagacctca tcacccaggt taagatcaag gtcttttcac     1680

ctggcccgca tggacaccca gaccaggtcc cctacatcgt gacctgggaa gccttggctt     1740

ttgacccccc tccctgggtc aagccctttg tacaccctaa gcctccgcct cctcttcctc     1800

catccgcccc gtctctcccc cttgaacctc ctcgttcgac cccgcctcga tcctcccttt     1860

atccagccct cactccttct ctaggcgccg gaattaattc tcgaggggcc cagatctgcg     1920

gccgcatggc cttaccagtg accgccttgc tcctgccgct ggccttgctg ctccacgccg     1980

ccaggccgga catccagatg acacagacta catcctccct gtctgcctct ctgggagaca     2040

gagtcaccat cagttgcagg gcaagtcagg acattagtaa atatttaaat tggtatcagc     2100

agaaaccaga tggaactgtt aaactcctga tctaccatac atcaagatta cactcaggag     2160

tcccatcaag gttcagtggc agtgggtctg gaacagatta ttctctcacc attagcaacc     2220

tggagcaaga agatattgcc acttactttt gccaacaggg taatacgctt ccgtacacgt     2280

tcggaggggg gaccaagctg gagatcacag gtggcggtgg ctcgggcggt ggtgggtcgg     2340

gtggcggcgg atctgaggtg aaactgcagg agtcaggacc tggcctggtg gcgccctcac     2400

agagcctgtc cgtcacatgc actgtctcag gggtctcatt acccgactat ggtgtaagct     2460

ggattcgcca gcctccacga aagggtctgg agtggctggg agtaatatgg ggtagtgaaa     2520

ccacatacta taattcagct ctcaaatcca gactgaccat catcaaggac aactccaaga     2580

gccaagtttt cttaaaaatg aacagtctgc aaactgatga cacagccatt tactactgtg     2640

ccaaacatta ttactacggt ggtagctatg ctatggacta ctggggccaa ggaacctcag     2700

tcaccgtctc ctcaaccacg acgccagcgc cgcgaccacc aacaccggcg cccaccatcg     2760

cgtcgcagcc cctgtccctg cgcccagagg cgtgccggcc agcggcgggg ggcgcagtgc     2820

acacgagggg gctggacttc gcctgtgata tctacatctg ggcgcccttg gccgggactt     2880

gtggggtcct tctcctgtca ctggttatca ccctttactg caaacggggc agaaagaaac     2940

tcctgtatat attcaaacaa ccatttatga gaccagtaca aactactcaa gaggaagatg     3000

gctgtagctg ccgatttcca gaagaagaag aaggaggatg tgaactgaga gtgaagttca     3060

gcaggagcgc agacgccccc gcgtacaagc agggccagaa ccagctctat aacgagctca     3120

atctaggacg aagagaggag tacgatgttt tggacaagag acgtggccgg gaccctgaga     3180

tggggggaaa gccgagaagg aagaaccctc aggaaggcct gtacaatgaa ctgcagaaag     3240

ataagatggc ggaggcctac agtgagattg ggatgaaagg cgagcgccgg aggggcaagg     3300

ggcacgatgg cctttaccag ggtctcagta cagccaccaa ggacacctac gacgcccttc     3360

acatgcaggc cctgccccct cgctaggtcg acacgcgtta ctggccgaag ccgcttggaa     3420

taaggccggt gtgcgtttgt ctatatgtta ttttccacca tattgccgtc ttttggcaat     3480

gtgagggccc ggaaacctgg ccctgtcttc ttgacgagca ttcctagggg tctttcccct     3540

ctcgccaaag gaatgcaagg tctgttgaat gtcgtgaagg aagcagttcc tctggaagct     3600

tcttgaagac aaacaacgtc tgtagcgacc ctttgcaggc agcggaaccc cccacctggc     3660

gacaggtgcc tctgcggcca aaagccacgt gtataagata cacctgcaaa ggcggcacaa     3720

ccccagtgcc acgttgtgag ttggatagtt gtggaaagag tcaaatggct ctcctcaagc     3780

gtattcaaca aggggctgaa ggatgcccag aaggtacccc attgtatggg atctgatctg     3840

gggcctcggt gcacatgctt tacatgtgtt tagtcgaggt taaaaaacgt ctaggccccc     3900

cgaaccacgg ggacgtggtt ttcctttgaa aaacacgatt ataaatggct tcatatcctt     3960

gccaccaaca tgcttccgct ttcgaccaag ccgcacggtc taggggccac aataatcgcc     4020

ggactgccct gcggcctcgg agacagcaga aggcaaccga agtcaggctc gagcaaaaga     4080

tgccaaccct cctgcgggtc tatatcgatg gaccccatgg aatggggaag accactacca     4140

cacaactcct ggtggcactc ggtagccggg acgacatcgt ctacgtgccc gaacccatga     4200

cttactggcg ggttctcggt gcttccgaga caatcgccaa tatctacaca acccaacacc     4260

gcctcgatca aggagaaatt agcgcagggg acgctgccgt ggtgatgaca tcagcccaaa     4320

tcaccatggg aatgccctac gccgtcaccg atgctgtcct ggcaccacac attggcggag     4380

aggccgggtc aagtcatgca ccaccaccag ccctgactat ctttctcgac cggcatccaa     4440

ttgcattcat gctgtgctat cctgccgcac gctacctgat gggaagtatg acaccacagg     4500

ccgtcctcgc cttcgttgct ctgatccctc caaccctgcc tggcactaac atcgttctcg     4560

gcgcactccc cgaagacaga cacattgatc ggctggccaa gaggcaacgg cctggcgaga     4620

gactcgatct ggctatgctg gctgctatta ggagagtgta cgggctgctg gccaatactg     4680

tgagatacct ccaaggggga ggaagctggc gcgaggattg gggccaactg tctggcgctg     4740

ctgtgccacc tcaaggcgcc gagccacagt caaatgctgg tcctaggccc cacatcggcg     4800

atactctctt tacactgttc cgggcaccag agctgctcgc acctaatgga gatctgtaca     4860

atgttttcgc ttgggccctc gatgtcctgg ctaagcggct ccggcctatg cacgtgttca     4920

tcctcgacta cgaccagagc ccagctggtt gtcgggatgc tctcctgcaa ctgaccagcg     4980

ggatggtgca gacacacgtt actactcccg gctccatccc cactatctgt gacctcgccc     5040

ggacatttgc ccgggaaatg ggcgaagcca actgatcgtt ggggtatttg acaaactgac     5100

acgtatgggg tatttgacaa actgacatcg atggggtatt tgacaaactg acatcactgg     5160

ggtatttgac aaactgacag tcgacgatca cgcgtaaggt cgggcaggaa gagggcctat     5220

ttcccatgat tccttcatat ttgcatatac gatacaaggc tgttagagag ataattagaa     5280

ttaatttgac tgtaaacaca aagatattag tacaaaatac gtgacgtaga aagtaataat     5340

ttcttgggta gtttgcagtt tttaaaatta tgttttaaaa tggactatca tatgcttacc     5400

gtaacttgaa agtatttcga tttcttggct ttatatatct tgtggaaagg acggatccgc     5460

cacagcagca cagaattctt tcaagagaag aattctgtgc tgctgtggct tttttagaaa     5520

caccgaagct tggatccatc gataaaataa aagattttat ttagtctcca gaaaaagggg     5580

ggaatgaaag accccacctg taggtttggc aagctagctt aagtaacgcc attttgcaag     5640

gcatggaaaa atacataact gagaatagag aagttcagat caaggtcagg aacagatgga     5700

acagctgaat atgggccaaa caggatatct gtggtaagca gttcctgccc cggctcaggg     5760

ccaagaacag atggaacagc tgaatatggg ccaaacagga tatctgtggt aagcagttcc     5820

tgccccggct cagggccaag aacagatggt ccccagatgc ggtccagccc tcagcagttt     5880

ctagagaacc atcagatgtt tccagggtgc cccaaggacc tgaaatgacc ctgtgcctta     5940

tttgaactaa ccaatcagtt cgcttctcgc ttctgttcgc gcgcttctgc tccccgagct     6000

caataaaaga gcccacaacc cctcactcgg cgcgccagtc ctccgattga ctgagtcgcc     6060

cgggtacccg tgtatccaat aaaccctctt gcagttgcat ccgacttgtg gtctcgctgt     6120

tccttgggag ggtctcctct gagtgattga ctacccgtca gcgggggtct ttcatttgaa     6180

gccgaattcg taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat     6240

tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag     6300

ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg     6360

ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc     6420

ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc     6480

agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa     6540

catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt     6600

tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg     6660

gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg     6720

ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag     6780

cgtggcgctt tctcaaagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc     6840

caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa     6900

ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg     6960

taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc     7020

taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac     7080

cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg     7140

tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt     7200

gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt     7260

catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa     7320

atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga     7380

ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt     7440

gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg     7500

agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga     7560

gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga     7620

agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg     7680

catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc     7740

aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc     7800

gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca     7860

taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac     7920

caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg     7980

ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc     8040

ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg     8100

tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac     8160

aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat     8220

actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata     8280

catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa     8340

agtgccacct gacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg     8400

tatcacgagg ccctttcgtc                                                 8420


<210>  11
<211>  5640
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Construct 51: pBA-9B-emdGFPmiRT199a-4TX

<400>  11
tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca       60

cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg      120

ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc      180

accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc      240

attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat      300

tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt      360

tttcccagtc acgacgttgt aaaacgacgg ccagtgccaa gctgactcta gaggatcgat      420

ccccggccgc tctagcttaa gtaacgccat tttgcaaggc atggaaaaat acataactga      480

gaatagagaa gttcagatca aggtcaggaa cagatggaac agctgaatat gggccaaaca      540

ggatatctgt ggtaagcagt tcctgccccg gctcagggcc aagaacagat ggaacagctg      600

aatatgggcc aaacaggata tctgtggtaa gcagttcctg ccccggctca gggccaagaa      660

cagatggtcc ccagatgcgg tccagccctc agcagtttct agagaaccat cagatgtttc      720

cagggtgccc caaggacctg aaatgaccct gtgccttatt tgaactaacc aatcagttcg      780

cttctcgctt ctgttcgcgc gcttctgctc cccgagctca ataaaagagc ccacaacccc      840

tcactcggcg cgccagtcct ccgattgact gagtcgcccg ggtacccgtg tatccaataa      900

accctcttgc agttgcatcc gacttgtggt ctcgctgttc cttgggaggg tctcctctga      960

gtgattgact acccgtcagc gggggtcttt catttggggg ctcgtccggg atcgggagac     1020

ccctgcccag ggaccaccga cccaccaccg ggaggtaagc tggccagcaa cttatctgtg     1080

tctgtccgat tgtctagtgt ctatgactga ttttatgcgc ctgcgtcggt actagttagc     1140

taactagctc tgtatctggc ggacccgtgg tggaactgac gagttcggaa cacccggccg     1200

caaccctggg agacgtccca gggacttcgg gggccgtttt tgtggcccga cctgagtcca     1260

aaaatcccga tcgttttgga ctctttggtg cacccccctt agaggaggga tatgtggttc     1320

tggtaggaga cgagaaccta aaacagttcc cgcctccgtc tgaatttttg ctttcggttt     1380

gggaccgaag ccgcgccgcg cgtcttgtct gctgcagcat cgttctgtgt tgtctctgtc     1440

tgactgtgtt tctgtatttg tctgagaatt aaggccagac tgttaccact ccctgaagtt     1500

tgaccttagg tcactggaaa gatgtcgagc ggatcgctca caaccagtcg gtagatgtca     1560

agaagagacg ttgggttacc ttctgctctg cagaatggcc aacctttaac gtcggatggc     1620

cgcgagacgg cacctttaac cgagacctca tcacccaggt taagatcaag gtcttttcac     1680

ctggcccgca tggacaccca gaccaggtcc cctacatcgt gacctgggaa gccttggctt     1740

ttgacccccc tccctgggtc aagccctttg tacaccctaa gcctccgcct cctcttcctc     1800

catccgcccc gtctctcccc cttgaacctc ctcgttcgac cccgcctcga tcctcccttt     1860

atccagccct cactccttct ctaggcgccg gaattaattc tcgagatggc cagcaagggc     1920

gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc     1980

cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa gctgaccctg     2040

aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccttg     2100

acctacggcg tgcagtgctt cgcccgctac cccgaccaca tgaagcagca cgacttcttc     2160

aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa ggacgacggc     2220

aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag     2280

ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac     2340

tacaacagcc acaaggtcta tatcaccgcc gacaagcaga agaacggcat caaggtgaac     2400

ttcaagaccc gccacaacat cgaggacggc agcgtgcagc tcgccgacca ctaccagcag     2460

aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagcacccag     2520

tccgccctga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg     2580

accgccgccg ggatcactct cggcatggac gagctgtaca agtgagaaca ggtagtctga     2640

acactggcga tgaacaggta gtctgaacac tgggcatgaa caggtagtct gaacactggc     2700

tacgaacagg tagtctgaac actgggcggc cgctcgcgag tcgacaagct tggatccatc     2760

gataaaataa aagattttat ttagtctcca gaaaaagggg ggaatgaaag accccacctg     2820

taggtttggc aagctagctt aagtaacgcc attttgcaag gcatggaaaa atacataact     2880

gagaatagag aagttcagat caaggtcagg aacagatgga acagctgaat atgggccaaa     2940

caggatatct gtggtaagca gttcctgccc cggctcaggg ccaagaacag atggaacagc     3000

tgaatatggg ccaaacagga tatctgtggt aagcagttcc tgccccggct cagggccaag     3060

aacagatggt ccccagatgc ggtccagccc tcagcagttt ctagagaacc atcagatgtt     3120

tccagggtgc cccaaggacc tgaaatgacc ctgtgcctta tttgaactaa ccaatcagtt     3180

cgcttctcgc ttctgttcgc gcgcttctgc tccccgagct caataaaaga gcccacaacc     3240

cctcactcgg cgcgccagtc ctccgattga ctgagtcgcc cgggtacccg tgtatccaat     3300

aaaccctctt gcagttgcat ccgacttgtg gtctcgctgt tccttgggag ggtctcctct     3360

gagtgattga ctacccgtca gcgggggtct ttcatttgaa gccgaattcg taatcatggt     3420

catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg     3480

gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt     3540

tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg     3600

gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg     3660

actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa     3720

tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc     3780

aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc     3840

ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat     3900

aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc     3960

cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcaaagct     4020

cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg     4080

aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc     4140

cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga     4200

ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa     4260

gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta     4320

gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc     4380

agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg     4440

acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga     4500

tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg     4560

agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct     4620

gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg     4680

agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc     4740

cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa     4800

ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc     4860

cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt     4920

cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc     4980

ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt     5040

tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc     5100

catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt     5160

gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata     5220

gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga     5280

tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag     5340

catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa     5400

aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt     5460

attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga     5520

aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtctaag     5580

aaaccattat tatcatgaca ttaacctata aaaataggcg tatcacgagg ccctttcgtc     5640


<210>  12
<211>  5540
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Construct 45: pBA-9b- GFP

<400>  12
tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca       60

cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg      120

ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc      180

accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc      240

attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat      300

tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt      360

tttcccagtc acgacgttgt aaaacgacgg ccagtgccaa gctgactcta gaggatcgat      420

ccccggccgc tctagcttaa gtaacgccat tttgcaaggc atggaaaaat acataactga      480

gaatagagaa gttcagatca aggtcaggaa cagatggaac agctgaatat gggccaaaca      540

ggatatctgt ggtaagcagt tcctgccccg gctcagggcc aagaacagat ggaacagctg      600

aatatgggcc aaacaggata tctgtggtaa gcagttcctg ccccggctca gggccaagaa      660

cagatggtcc ccagatgcgg tccagccctc agcagtttct agagaaccat cagatgtttc      720

cagggtgccc caaggacctg aaatgaccct gtgccttatt tgaactaacc aatcagttcg      780

cttctcgctt ctgttcgcgc gcttctgctc cccgagctca ataaaagagc ccacaacccc      840

tcactcggcg cgccagtcct ccgattgact gagtcgcccg ggtacccgtg tatccaataa      900

accctcttgc agttgcatcc gacttgtggt ctcgctgttc cttgggaggg tctcctctga      960

gtgattgact acccgtcagc gggggtcttt catttggggg ctcgtccggg atcgggagac     1020

ccctgcccag ggaccaccga cccaccaccg ggaggtaagc tggccagcaa cttatctgtg     1080

tctgtccgat tgtctagtgt ctatgactga ttttatgcgc ctgcgtcggt actagttagc     1140

taactagctc tgtatctggc ggacccgtgg tggaactgac gagttcggaa cacccggccg     1200

caaccctggg agacgtccca gggacttcgg gggccgtttt tgtggcccga cctgagtcca     1260

aaaatcccga tcgttttgga ctctttggtg cacccccctt agaggaggga tatgtggttc     1320

tggtaggaga cgagaaccta aaacagttcc cgcctccgtc tgaatttttg ctttcggttt     1380

gggaccgaag ccgcgccgcg cgtcttgtct gctgcagcat cgttctgtgt tgtctctgtc     1440

tgactgtgtt tctgtatttg tctgagaatt aaggccagac tgttaccact ccctgaagtt     1500

tgaccttagg tcactggaaa gatgtcgagc ggatcgctca caaccagtcg gtagatgtca     1560

agaagagacg ttgggttacc ttctgctctg cagaatggcc aacctttaac gtcggatggc     1620

cgcgagacgg cacctttaac cgagacctca tcacccaggt taagatcaag gtcttttcac     1680

ctggcccgca tggacaccca gaccaggtcc cctacatcgt gacctgggaa gccttggctt     1740

ttgacccccc tccctgggtc aagccctttg tacaccctaa gcctccgcct cctcttcctc     1800

catccgcccc gtctctcccc cttgaacctc ctcgttcgac cccgcctcga tcctcccttt     1860

atccagccct cactccttct ctaggcgccg gaattaattc tcgagatggc cagcaagggc     1920

gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc     1980

cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa gctgaccctg     2040

aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccttg     2100

acctacggcg tgcagtgctt cgcccgctac cccgaccaca tgaagcagca cgacttcttc     2160

aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa ggacgacggc     2220

aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag     2280

ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac     2340

tacaacagcc acaaggtcta tatcaccgcc gacaagcaga agaacggcat caaggtgaac     2400

ttcaagaccc gccacaacat cgaggacggc agcgtgcagc tcgccgacca ctaccagcag     2460

aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagcacccag     2520

tccgccctga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg     2580

accgccgccg ggatcactct cggcatggac gagctgtaca agtgagcggc cgctcgcgag     2640

tcgacaagct tggatccatc gataaaataa aagattttat ttagtctcca gaaaaagggg     2700

ggaatgaaag accccacctg taggtttggc aagctagctt aagtaacgcc attttgcaag     2760

gcatggaaaa atacataact gagaatagag aagttcagat caaggtcagg aacagatgga     2820

acagctgaat atgggccaaa caggatatct gtggtaagca gttcctgccc cggctcaggg     2880

ccaagaacag atggaacagc tgaatatggg ccaaacagga tatctgtggt aagcagttcc     2940

tgccccggct cagggccaag aacagatggt ccccagatgc ggtccagccc tcagcagttt     3000

ctagagaacc atcagatgtt tccagggtgc cccaaggacc tgaaatgacc ctgtgcctta     3060

tttgaactaa ccaatcagtt cgcttctcgc ttctgttcgc gcgcttctgc tccccgagct     3120

caataaaaga gcccacaacc cctcactcgg cgcgccagtc ctccgattga ctgagtcgcc     3180

cgggtacccg tgtatccaat aaaccctctt gcagttgcat ccgacttgtg gtctcgctgt     3240

tccttgggag ggtctcctct gagtgattga ctacccgtca gcgggggtct ttcatttgaa     3300

gccgaattcg taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat     3360

tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag     3420

ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg     3480

ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc     3540

ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc     3600

agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa     3660

catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt     3720

tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg     3780

gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg     3840

ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag     3900

cgtggcgctt tctcaaagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc     3960

caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa     4020

ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg     4080

taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc     4140

taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac     4200

cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg     4260

tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt     4320

gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt     4380

catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa     4440

atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga     4500

ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt     4560

gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg     4620

agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga     4680

gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga     4740

agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg     4800

catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc     4860

aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc     4920

gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca     4980

taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac     5040

caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg     5100

ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc     5160

ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg     5220

tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac     5280

aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat     5340

actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata     5400

catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa     5460

agtgccacct gacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg     5520

tatcacgagg ccctttcgtc                                                 5540


<210>  13
<211>  9519
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Construct 41: pBA-9b mCD19CAR-IRES yCD-CMVprom-mIL12P70

<400>  13
tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca       60

cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg      120

ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc      180

accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc      240

attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat      300

tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt      360

tttcccagtc acgacgttgt aaaacgacgg ccagtgccaa gctgactcta gaggatcgat      420

ccccggccgc tctagcttaa gtaacgccat tttgcaaggc atggaaaaat acataactga      480

gaatagagaa gttcagatca aggtcaggaa cagatggaac agctgaatat gggccaaaca      540

ggatatctgt ggtaagcagt tcctgccccg gctcagggcc aagaacagat ggaacagctg      600

aatatgggcc aaacaggata tctgtggtaa gcagttcctg ccccggctca gggccaagaa      660

cagatggtcc ccagatgcgg tccagccctc agcagtttct agagaaccat cagatgtttc      720

cagggtgccc caaggacctg aaatgaccct gtgccttatt tgaactaacc aatcagttcg      780

cttctcgctt ctgttcgcgc gcttctgctc cccgagctca ataaaagagc ccacaacccc      840

tcactcggcg cgccagtcct ccgattgact gagtcgcccg ggtacccgtg tatccaataa      900

accctcttgc agttgcatcc gacttgtggt ctcgctgttc cttgggaggg tctcctctga      960

gtgattgact acccgtcagc gggggtcttt catttggggg ctcgtccggg atcgggagac     1020

ccctgcccag ggaccaccga cccaccaccg ggaggtaagc tggccagcaa cttatctgtg     1080

tctgtccgat tgtctagtgt ctatgactga ttttatgcgc ctgcgtcggt actagttagc     1140

taactagctc tgtatctggc ggacccgtgg tggaactgac gagttcggaa cacccggccg     1200

caaccctggg agacgtccca gggacttcgg gggccgtttt tgtggcccga cctgagtcca     1260

aaaatcccga tcgttttgga ctctttggtg cacccccctt agaggaggga tatgtggttc     1320

tggtaggaga cgagaaccta aaacagttcc cgcctccgtc tgaatttttg ctttcggttt     1380

gggaccgaag ccgcgccgcg cgtcttgtct gctgcagcat cgttctgtgt tgtctctgtc     1440

tgactgtgtt tctgtatttg tctgagaatt aaggccagac tgttaccact ccctgaagtt     1500

tgaccttagg tcactggaaa gatgtcgagc ggatcgctca caaccagtcg gtagatgtca     1560

agaagagacg ttgggttacc ttctgctctg cagaatggcc aacctttaac gtcggatggc     1620

cgcgagacgg cacctttaac cgagacctca tcacccaggt taagatcaag gtcttttcac     1680

ctggcccgca tggacaccca gaccaggtcc cctacatcgt gacctgggaa gccttggctt     1740

ttgacccccc tccctgggtc aagccctttg tacaccctaa gcctccgcct cctcttcctc     1800

catccgcccc gtctctcccc cttgaacctc ctcgttcgac cccgcctcga tcctcccttt     1860

atccagccct cactccttct ctaggcgccg gaattaattc tcgaggggcc cagatctgcg     1920

gccgcatggg cgtgcccacc cagctgctgg gcctgctgct gctgtggatc accgacgcca     1980

tctgcgacat ccagatgacc cagagccctg ccagcctgag caccagcctg ggcgagaccg     2040

tgaccatcca gtgccaggcc agcgaggaca tctacagcgg cctggcctgg taccagcaga     2100

agcctggcaa gagccctcag ctgctgatct acggcgccag cgacctgcag gacggcgtgc     2160

ctagcaggtt cagcggcagc ggcagcggca cccagtacag cctgaagatc accagcatgc     2220

agaccgagga cgagggcgtg tacttctgcc agcagggcct gacctaccct aggaccttcg     2280

gcggcggcac caagctggag ctgaagggcg gcggcggcag cggcggcggc ggcagcggcg     2340

gcggcggcag cgaggtgcag ctgcagcaga gcggcgccga gctggtgagg cctggcacca     2400

gcgtgaagct gagctgcaag gtgagcggcg acaccatcac cttctactac atgcacttcg     2460

tgaagcagag gcctggccag ggcctggagt ggatcggcag gatcgaccct gaggacgaga     2520

gcaccaagta cagcgagaag ttcaagaaca aggccaccct gaccgccgac accagcagca     2580

acaccgccta cctgaagctg agcagcctga ccagcgagga caccgccacc tacttctgca     2640

tctacggcgg ctactacttc gactactggg gccagggcgt gatggtgacc gtgagcagca     2700

ttgagttcat gtaccctccg ccttacctag acaacgagag gagcaatgga actattattc     2760

acataaaaga gaaacatctt tgtcatactc agtcatctcc taagctgttt tgggcactgg     2820

tcgtggttgc tggagtcctg ttttgttatg gcttgctagt gacagtggct ctttgtgtta     2880

tctggacaaa atggatcagg aaaaaattcc cccacatatt caagcaacca tttaagaaga     2940

ccactggagc agctcaagag gaagatgctt gtagctgccg atgtccacag gaagaagaag     3000

gaggaggagg aggctatgag ctgagagcaa aattcagcag gagtgcagag actgctgcca     3060

acctgcagga ccccaaccag ctctacaatg agctcaatct agggcgaaga gaggaatatg     3120

acgtcttgga gaagaagcgg gctcgggatc cagagatggg aggcaaacag cagaggagga     3180

ggaaccccca ggaaggcgta tacaatgcac tgcagaaaga caagatggca gaagcctaca     3240

gtgagatcgg cacaaaaggc gagaggcgga gaggcaaggg gcacgatggc ctttaccagg     3300

gtctcagcac tgccaccaag gacacctatg atgccctgca tatgcagacc ctggcccctc     3360

gctgagtcga cacgcgttac tggccgaagc cgcttggaat aaggccggtg tgcgtttgtc     3420

tatatgttat tttccaccat attgccgtct tttggcaatg tgagggcccg gaaacctggc     3480

cctgtcttct tgacgagcat tcctaggggt ctttcccctc tcgccaaagg aatgcaaggt     3540

ctgttgaatg tcgtgaagga agcagttcct ctggaagctt cttgaagaca aacaacgtct     3600

gtagcgaccc tttgcaggca gcggaacccc ccacctggcg acaggtgcct ctgcggccaa     3660

aagccacgtg tataagatac acctgcaaag gcggcacaac cccagtgcca cgttgtgagt     3720

tggatagttg tggaaagagt caaatggctc tcctcaagcg tattcaacaa ggggctgaag     3780

gatgcccaga aggtacccca ttgtatggga tctgatctgg ggcctcggtg cacatgcttt     3840

acatgtgttt agtcgaggtt aaaaaacgtc taggcccccc gaaccacggg gacgtggttt     3900

tcctttgaaa aacacgatta taaatggtga ccggcggcat ggcctccaag tgggatcaaa     3960

agggcatgga tatcgcttac gaggaggccc tgctgggcta caaggagggc ggcgtgccta     4020

tcggcggctg tctgatcaac aacaaggacg gcagtgtgct gggcaggggc cacaacatga     4080

ggttccagaa gggctccgcc accctgcacg gcgagatctc caccctggag aactgtggca     4140

ggctggaggg caaggtgtac aaggacacca ccctgtacac caccctgtcc ccttgtgaca     4200

tgtgtaccgg cgctatcatc atgtacggca tccctaggtg tgtgatcggc gagaacgtga     4260

acttcaagtc caagggcgag aagtacctgc aaaccagggg ccacgaggtg gtggttgttg     4320

acgatgagag gtgtaagaag ctgatgaagc agttcatcga cgagaggcct caggactggt     4380

tcgaggatat cggcgagtaa attagtcgac tgcgctgaca ttgattattg actagttatt     4440

aatagtaatc aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat     4500

aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa     4560

taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg     4620

agtatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc     4680

cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct     4740

tatgggactt tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga     4800

tgcggttttg gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa     4860

gtctccaccc cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc     4920

caaaatgtcg taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg     4980

aggtctatat aagcagagct gccacccaag atgggtcctc agaagctaac catctcctgg     5040

tttgccatcg ttttgctggt gtctccactc atggccatgt gggagctgga gaaagacgtt     5100

tatgttgtag aggtggactg gactcccgat gcccctggag aaacagtgaa cctcacctgt     5160

gacacgcctg aagaagatga catcacctgg acctcagacc agagacatgg agtcataggc     5220

tctggaaaga ccctgaccat cactgtcaaa gagtttctag atgctggcca gtacacctgc     5280

cacaaaggag gcgagactct gagccactca catctgctgc tccacaagaa ggaaaatgga     5340

atttggtcca ctgaaatttt aaaaaatttc aaaaacaaga ctttcctgaa gtgtgaagca     5400

ccaaattact ccggacggtt cacgtgctca tggctggtgc aaagaaacat ggacttgaag     5460

ttcaacatca agagcagtag cagttcccct gactctcggg cagtgacatg tggaatggcg     5520

tctctgtctg cagagaaggt cacactggac caaagggact atgagaagta ttcagtgtcc     5580

tgccaggagg atgtcacctg cccaactgcc gaggagaccc tgcccattga actggcgttg     5640

gaagcacggc agcagaataa atatgagaac tacagcacca gcttcttcat cagggacatc     5700

atcaaaccag acccgcccaa gaacttgcag atgaagcctt tgaagaactc acaggtggag     5760

gtcagctggg agtaccctga ctcctggagc actccccatt cctacttctc cctcaagttc     5820

tttgttcgaa tccagcgcaa gaaagaaaag atgaaggaga cagaggaggg gtgtaaccag     5880

aaaggtgcgt tcctcgtaga gaagacatct accgaagtcc aatgcaaagg cgggaatgtc     5940

tgcgtgcaag ctcaggatcg ctattacaat tcctcgtgca gcaagtgggc atgtgttccc     6000

tgcagggtcc gatccgtccg atccggtggc ggtggctcgg gcggtggtgg gtcgggtggc     6060

ggcggatcta gggtcattcc aagggtcatt ccagtctctg gacctgccag gtgtcttagc     6120

cagtcccgaa acctgctgaa gaccacagat gacatggtga agacggccag agaaaaactg     6180

aaacattatt cctgcactgc tgaagacatc gatcatgaag acatcacacg ggaccaaacc     6240

agcacattga agacctgttt accactggaa ctacacaaga acgagagttg cctggctact     6300

agagagactt cttccacaac aagagggagc tgcctgcccc cacagaagac gtctttgatg     6360

atgaccctgt gccttggtag catctatgag gacttgaaga tgtaccagac agagttccag     6420

gccatcaacg cagcacttca gaatcacaac catcagcaga tcattctaga caagggcatg     6480

ctggtggcca tcgatgagct gatgcagtct ctgaatcata atggcgagac tctgcgccag     6540

aaacctcctg tgggagaagc agacccttac agagtgaaaa tgaagctctg catcctgctt     6600

cacgccttca gcacccgcgt cgtgaccatc aacagggtga tgggctatct gagctccgcc     6660

taatctccag aaaaaggggg gaatgaaaga ccccacctgt aggtttggca agctagctta     6720

agtaacgcca ttttgcaagg catggaaaaa tacataactg agaatagaga agttcagatc     6780

aaggtcagga acagatggaa cagctgaata tgggccaaac aggatatctg tggtaagcag     6840

ttcctgcccc ggctcagggc caagaacaga tggaacagct gaatatgggc caaacaggat     6900

atctgtggta agcagttcct gccccggctc agggccaaga acagatggtc cccagatgcg     6960

gtccagccct cagcagtttc tagagaacca tcagatgttt ccagggtgcc ccaaggacct     7020

gaaatgaccc tgtgccttat ttgaactaac caatcagttc gcttctcgct tctgttcgcg     7080

cgcttctgct ccccgagctc aataaaagag cccacaaccc ctcactcggc gcgccagtcc     7140

tccgattgac tgagtcgccc gggtacccgt gtatccaata aaccctcttg cagttgcatc     7200

cgacttgtgg tctcgctgtt ccttgggagg gtctcctctg agtgattgac tacccgtcag     7260

cgggggtctt tcatttgaag ccgaattcgt aatcatggtc atagctgttt cctgtgtgaa     7320

attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct     7380

ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc     7440

agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg     7500

gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc     7560

ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag     7620

gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa     7680

aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc     7740

gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc     7800

ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg     7860

cctttctccc ttcgggaagc gtggcgcttt ctcaaagctc acgctgtagg tatctcagtt     7920

cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc     7980

gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc     8040

cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag     8100

agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg     8160

ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa     8220

ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag     8280

gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact     8340

cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa     8400

attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt     8460

accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag     8520

ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca     8580

gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc     8640

agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt     8700

ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg     8760

ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca     8820

gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg     8880

ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca     8940

tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg     9000

tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct     9060

cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca     9120

tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca     9180

gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg     9240

tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac     9300

ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt     9360

attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc     9420

cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga aaccattatt atcatgacat     9480

taacctataa aaataggcgt atcacgaggc cctttcgtc                            9519


<210>  14
<211>  8351
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Construct 42: pBA-9b-mCD19CAR-IRES-TKmiRT+CMVprom-IL2F42A

<400>  14
tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca       60

cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg      120

ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc      180

accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc      240

attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat      300

tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt      360

tttcccagtc acgacgttgt aaaacgacgg ccagtgccaa gctgactcta gaggatcgat      420

ccccggccgc tctagcttaa gtaacgccat tttgcaaggc atggaaaaat acataactga      480

gaatagagaa gttcagatca aggtcaggaa cagatggaac agctgaatat gggccaaaca      540

ggatatctgt ggtaagcagt tcctgccccg gctcagggcc aagaacagat ggaacagctg      600

aatatgggcc aaacaggata tctgtggtaa gcagttcctg ccccggctca gggccaagaa      660

cagatggtcc ccagatgcgg tccagccctc agcagtttct agagaaccat cagatgtttc      720

cagggtgccc caaggacctg aaatgaccct gtgccttatt tgaactaacc aatcagttcg      780

cttctcgctt ctgttcgcgc gcttctgctc cccgagctca ataaaagagc ccacaacccc      840

tcactcggcg cgccagtcct ccgattgact gagtcgcccg ggtacccgtg tatccaataa      900

accctcttgc agttgcatcc gacttgtggt ctcgctgttc cttgggaggg tctcctctga      960

gtgattgact acccgtcagc gggggtcttt catttggggg ctcgtccggg atcgggagac     1020

ccctgcccag ggaccaccga cccaccaccg ggaggtaagc tggccagcaa cttatctgtg     1080

tctgtccgat tgtctagtgt ctatgactga ttttatgcgc ctgcgtcggt actagttagc     1140

taactagctc tgtatctggc ggacccgtgg tggaactgac gagttcggaa cacccggccg     1200

caaccctggg agacgtccca gggacttcgg gggccgtttt tgtggcccga cctgagtcca     1260

aaaatcccga tcgttttgga ctctttggtg cacccccctt agaggaggga tatgtggttc     1320

tggtaggaga cgagaaccta aaacagttcc cgcctccgtc tgaatttttg ctttcggttt     1380

gggaccgaag ccgcgccgcg cgtcttgtct gctgcagcat cgttctgtgt tgtctctgtc     1440

tgactgtgtt tctgtatttg tctgagaatt aaggccagac tgttaccact ccctgaagtt     1500

tgaccttagg tcactggaaa gatgtcgagc ggatcgctca caaccagtcg gtagatgtca     1560

agaagagacg ttgggttacc ttctgctctg cagaatggcc aacctttaac gtcggatggc     1620

cgcgagacgg cacctttaac cgagacctca tcacccaggt taagatcaag gtcttttcac     1680

ctggcccgca tggacaccca gaccaggtcc cctacatcgt gacctgggaa gccttggctt     1740

ttgacccccc tccctgggtc aagccctttg tacaccctaa gcctccgcct cctcttcctc     1800

catccgcccc gtctctcccc cttgaacctc ctcgttcgac cccgcctcga tcctcccttt     1860

atccagccct cactccttct ctaggcgccg gaattaattc tcgaggggcc cagatctgcg     1920

gccgcatggg cgtgcccacc cagctgctgg gcctgctgct gctgtggatc accgacgcca     1980

tctgcgacat ccagatgacc cagagccctg ccagcctgag caccagcctg ggcgagaccg     2040

tgaccatcca gtgccaggcc agcgaggaca tctacagcgg cctggcctgg taccagcaga     2100

agcctggcaa gagccctcag ctgctgatct acggcgccag cgacctgcag gacggcgtgc     2160

ctagcaggtt cagcggcagc ggcagcggca cccagtacag cctgaagatc accagcatgc     2220

agaccgagga cgagggcgtg tacttctgcc agcagggcct gacctaccct aggaccttcg     2280

gcggcggcac caagctggag ctgaagggcg gcggcggcag cggcggcggc ggcagcggcg     2340

gcggcggcag cgaggtgcag ctgcagcaga gcggcgccga gctggtgagg cctggcacca     2400

gcgtgaagct gagctgcaag gtgagcggcg acaccatcac cttctactac atgcacttcg     2460

tgaagcagag gcctggccag ggcctggagt ggatcggcag gatcgaccct gaggacgaga     2520

gcaccaagta cagcgagaag ttcaagaaca aggccaccct gaccgccgac accagcagca     2580

acaccgccta cctgaagctg agcagcctga ccagcgagga caccgccacc tacttctgca     2640

tctacggcgg ctactacttc gactactggg gccagggcgt gatggtgacc gtgagcagca     2700

ttgagttcat gtaccctccg ccttacctag acaacgagag gagcaatgga actattattc     2760

acataaaaga gaaacatctt tgtcatactc agtcatctcc taagctgttt tgggcactgg     2820

tcgtggttgc tggagtcctg ttttgttatg gcttgctagt gacagtggct ctttgtgtta     2880

tctggacaaa atggatcagg aaaaaattcc cccacatatt caagcaacca tttaagaaga     2940

ccactggagc agctcaagag gaagatgctt gtagctgccg atgtccacag gaagaagaag     3000

gaggaggagg aggctatgag ctgagagcaa aattcagcag gagtgcagag actgctgcca     3060

acctgcagga ccccaaccag ctctacaatg agctcaatct agggcgaaga gaggaatatg     3120

acgtcttgga gaagaagcgg gctcgggatc cagagatggg aggcaaacag cagaggagga     3180

ggaaccccca ggaaggcgta tacaatgcac tgcagaaaga caagatggca gaagcctaca     3240

gtgagatcgg cacaaaaggc gagaggcgga gaggcaaggg gcacgatggc ctttaccagg     3300

gtctcagcac tgccaccaag gacacctatg atgccctgca tatgcagacc ctggcccctc     3360

gctgagtcga cacgcgttac tggccgaagc cgcttggaat aaggccggtg tgcgtttgtc     3420

tatatgttat tttccaccat attgccgtct tttggcaatg tgagggcccg gaaacctggc     3480

cctgtcttct tgacgagcat tcctaggggt ctttcccctc tcgccaaagg aatgcaaggt     3540

ctgttgaatg tcgtgaagga agcagttcct ctggaagctt cttgaagaca aacaacgtct     3600

gtagcgaccc tttgcaggca gcggaacccc ccacctggcg acaggtgcct ctgcggccaa     3660

aagccacgtg tataagatac acctgcaaag gcggcacaac cccagtgcca cgttgtgagt     3720

tggatagttg tggaaagagt caaatggctc tcctcaagcg tattcaacaa ggggctgaag     3780

gatgcccaga aggtacccca ttgtatggga tctgatctgg ggcctcggtg cacatgcttt     3840

acatgtgttt agtcgaggtt aaaaaacgtc taggcccccc gaaccacggg gacgtggttt     3900

tcctttgaaa aacacgatta taaatggtga ccggcggcat ggcctccaag tgggatcaaa     3960

agggcatgga tatcgcttac gaggaggccc tgctgggcta caaggagggc ggcgtgccta     4020

tcggcggctg tctgatcaac aacaaggacg gcagtgtgct gggcaggggc cacaacatga     4080

ggttccagaa gggctccgcc accctgcacg gcgagatctc caccctggag aactgtggca     4140

ggctggaggg caaggtgtac aaggacacca ccctgtacac caccctgtcc ccttgtgaca     4200

tgtgtaccgg cgctatcatc atgtacggca tccctaggtg tgtgatcggc gagaacgtga     4260

acttcaagtc caagggcgag aagtacctgc aaaccagggg ccacgaggtg gtggttgttg     4320

acgatgagag gtgtaagaag ctgatgaagc agttcatcga cgagaggcct caggactggt     4380

tcgaggatat cggcgagtaa attagtcgac tgcgctgaca ttgattattg actagttatt     4440

aatagtaatc aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat     4500

aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa     4560

taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg     4620

agtatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc     4680

cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct     4740

tatgggactt tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga     4800

tgcggttttg gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa     4860

gtctccaccc cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc     4920

caaaatgtcg taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg     4980

aggtctatat aagcagagct gccaccatgg taagcgctat tgttttatat gtgcttttgg     5040

cggcggcggc gcattctgcc tttgcggcgg atccagcacc tacttcaagt tctacaaaga     5100

aaacacagct acaactggag catttacttc tggatttaca gatgattttg aatggaatta     5160

ataattacaa gaatcccaaa ctcaccagga tgctcacagc aaagttttac atgcccaaga     5220

aggccacaga actgaaacat cttcagtgtc tagaagaaga actcaaacct ctggaggaag     5280

tgctaaattt agctcaaagc aaaaactttc acttaagacc cagggactta atcagcaata     5340

tcaacgtaat agttctggaa ctaaagggat ctgaaacaac attcatgtgt gaatatgctg     5400

atgagacagc aaccattgta gaatttctga acagatggat taccttttgt caaagcatca     5460

tctcaacact aactcatcat caccatcacc attgatctcc agaaaaaggg gggaatgaaa     5520

gaccccacct gtaggtttgg caagctagct taagtaacgc cattttgcaa ggcatggaaa     5580

aatacataac tgagaataga gaagttcaga tcaaggtcag gaacagatgg aacagctgaa     5640

tatgggccaa acaggatatc tgtggtaagc agttcctgcc ccggctcagg gccaagaaca     5700

gatggaacag ctgaatatgg gccaaacagg atatctgtgg taagcagttc ctgccccggc     5760

tcagggccaa gaacagatgg tccccagatg cggtccagcc ctcagcagtt tctagagaac     5820

catcagatgt ttccagggtg ccccaaggac ctgaaatgac cctgtgcctt atttgaacta     5880

accaatcagt tcgcttctcg cttctgttcg cgcgcttctg ctccccgagc tcaataaaag     5940

agcccacaac ccctcactcg gcgcgccagt cctccgattg actgagtcgc ccgggtaccc     6000

gtgtatccaa taaaccctct tgcagttgca tccgacttgt ggtctcgctg ttccttggga     6060

gggtctcctc tgagtgattg actacccgtc agcgggggtc tttcatttga agccgaattc     6120

gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa     6180

catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac     6240

attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca     6300

ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc     6360

ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc     6420

aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc     6480

aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag     6540

gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc     6600

gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt     6660

tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct     6720

ttctcaaagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg     6780

ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct     6840

tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat     6900

tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg     6960

ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa     7020

aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt     7080

ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc     7140

tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt     7200

atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta     7260

aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat     7320

ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac     7380

tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg     7440

ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag     7500

tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt     7560

aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt     7620

gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt     7680

tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt     7740

cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct     7800

tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt     7860

ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac     7920

cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa     7980

actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa     8040

ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca     8100

aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct     8160

ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga     8220

atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc     8280

tgacgtctaa gaaaccatta ttatcatgac attaacctat aaaaataggc gtatcacgag     8340

gccctttcgt c                                                          8351


<210>  15
<211>  9252
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Construct 9: pBA-9b-hCD19CAR miRTCMVpromIL15reverse

<400>  15
tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca       60

cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg      120

ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc      180

accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc      240

attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat      300

tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt      360

tttcccagtc acgacgttgt aaaacgacgg ccagtgccaa gctgactcta gaggatcgat      420

ccccggccgc tctagcttaa gtaacgccat tttgcaaggc atggaaaaat acataactga      480

gaatagagaa gttcagatca aggtcaggaa cagatggaac agctgaatat gggccaaaca      540

ggatatctgt ggtaagcagt tcctgccccg gctcagggcc aagaacagat ggaacagctg      600

aatatgggcc aaacaggata tctgtggtaa gcagttcctg ccccggctca gggccaagaa      660

cagatggtcc ccagatgcgg tccagccctc agcagtttct agagaaccat cagatgtttc      720

cagggtgccc caaggacctg aaatgaccct gtgccttatt tgaactaacc aatcagttcg      780

cttctcgctt ctgttcgcgc gcttctgctc cccgagctca ataaaagagc ccacaacccc      840

tcactcggcg cgccagtcct ccgattgact gagtcgcccg ggtacccgtg tatccaataa      900

accctcttgc agttgcatcc gacttgtggt ctcgctgttc cttgggaggg tctcctctga      960

gtgattgact acccgtcagc gggggtcttt catttggggg ctcgtccggg atcgggagac     1020

ccctgcccag ggaccaccga cccaccaccg ggaggtaagc tggccagcaa cttatctgtg     1080

tctgtccgat tgtctagtgt ctatgactga ttttatgcgc ctgcgtcggt actagttagc     1140

taactagctc tgtatctggc ggacccgtgg tggaactgac gagttcggaa cacccggccg     1200

caaccctggg agacgtccca gggacttcgg gggccgtttt tgtggcccga cctgagtcca     1260

aaaatcccga tcgttttgga ctctttggtg cacccccctt agaggaggga tatgtggttc     1320

tggtaggaga cgagaaccta aaacagttcc cgcctccgtc tgaatttttg ctttcggttt     1380

gggaccgaag ccgcgccgcg cgtcttgtct gctgcagcat cgttctgtgt tgtctctgtc     1440

tgactgtgtt tctgtatttg tctgagaatt aaggccagac tgttaccact ccctgaagtt     1500

tgaccttagg tcactggaaa gatgtcgagc ggatcgctca caaccagtcg gtagatgtca     1560

agaagagacg ttgggttacc ttctgctctg cagaatggcc aacctttaac gtcggatggc     1620

cgcgagacgg cacctttaac cgagacctca tcacccaggt taagatcaag gtcttttcac     1680

ctggcccgca tggacaccca gaccaggtcc cctacatcgt gacctgggaa gccttggctt     1740

ttgacccccc tccctgggtc aagccctttg tacaccctaa gcctccgcct cctcttcctc     1800

catccgcccc gtctctcccc cttgaacctc ctcgttcgac cccgcctcga tcctcccttt     1860

atccagccct cactccttct ctaggcgccg gaattaattc tcgaggggcc cagatctgcg     1920

gccgcatggc cttaccagtg accgccttgc tcctgccgct ggccttgctg ctccacgccg     1980

ccaggccgga catccagatg acacagacta catcctccct gtctgcctct ctgggagaca     2040

gagtcaccat cagttgcagg gcaagtcagg acattagtaa atatttaaat tggtatcagc     2100

agaaaccaga tggaactgtt aaactcctga tctaccatac atcaagatta cactcaggag     2160

tcccatcaag gttcagtggc agtgggtctg gaacagatta ttctctcacc attagcaacc     2220

tggagcaaga agatattgcc acttactttt gccaacaggg taatacgctt ccgtacacgt     2280

tcggaggggg gaccaagctg gagatcacag gtggcggtgg ctcgggcggt ggtgggtcgg     2340

gtggcggcgg atctgaggtg aaactgcagg agtcaggacc tggcctggtg gcgccctcac     2400

agagcctgtc cgtcacatgc actgtctcag gggtctcatt acccgactat ggtgtaagct     2460

ggattcgcca gcctccacga aagggtctgg agtggctggg agtaatatgg ggtagtgaaa     2520

ccacatacta taattcagct ctcaaatcca gactgaccat catcaaggac aactccaaga     2580

gccaagtttt cttaaaaatg aacagtctgc aaactgatga cacagccatt tactactgtg     2640

ccaaacatta ttactacggt ggtagctatg ctatggacta ctggggccaa ggaacctcag     2700

tcaccgtctc ctcaaccacg acgccagcgc cgcgaccacc aacaccggcg cccaccatcg     2760

cgtcgcagcc cctgtccctg cgcccagagg cgtgccggcc agcggcgggg ggcgcagtgc     2820

acacgagggg gctggacttc gcctgtgata tctacatctg ggcgcccttg gccgggactt     2880

gtggggtcct tctcctgtca ctggttatca ccctttactg caaacggggc agaaagaaac     2940

tcctgtatat attcaaacaa ccatttatga gaccagtaca aactactcaa gaggaagatg     3000

gctgtagctg ccgatttcca gaagaagaag aaggaggatg tgaactgaga gtgaagttca     3060

gcaggagcgc agacgccccc gcgtacaagc agggccagaa ccagctctat aacgagctca     3120

atctaggacg aagagaggag tacgatgttt tggacaagag acgtggccgg gaccctgaga     3180

tggggggaaa gccgagaagg aagaaccctc aggaaggcct gtacaatgaa ctgcagaaag     3240

ataagatggc ggaggcctac agtgagattg ggatgaaagg cgagcgccgg aggggcaagg     3300

ggcacgatgg cctttaccag ggtctcagta cagccaccaa ggacacctac gacgcccttc     3360

acatgcaggc cctgccccct cgctaggtcg acacgcgtta ctggccgaag ccgcttggaa     3420

taaggccggt gtgcgtttgt ctatatgtta ttttccacca tattgccgtc ttttggcaat     3480

gtgagggccc ggaaacctgg ccctgtcttc ttgacgagca ttcctagggg tctttcccct     3540

ctcgccaaag gaatgcaagg tctgttgaat gtcgtgaagg aagcagttcc tctggaagct     3600

tcttgaagac aaacaacgtc tgtagcgacc ctttgcaggc agcggaaccc cccacctggc     3660

gacaggtgcc tctgcggcca aaagccacgt gtataagata cacctgcaaa ggcggcacaa     3720

ccccagtgcc acgttgtgag ttggatagtt gtggaaagag tcaaatggct ctcctcaagc     3780

gtattcaaca aggggctgaa ggatgcccag aaggtacccc attgtatggg atctgatctg     3840

gggcctcggt gcacatgctt tacatgtgtt tagtcgaggt taaaaaacgt ctaggccccc     3900

cgaaccacgg ggacgtggtt ttcctttgaa aaacacgatt ataaatggct tcatatcctt     3960

gccaccaaca tgcttccgct ttcgaccaag ccgcacggtc taggggccac aataatcgcc     4020

ggactgccct gcggcctcgg agacagcaga aggcaaccga agtcaggctc gagcaaaaga     4080

tgccaaccct cctgcgggtc tatatcgatg gaccccatgg aatggggaag accactacca     4140

cacaactcct ggtggcactc ggtagccggg acgacatcgt ctacgtgccc gaacccatga     4200

cttactggcg ggttctcggt gcttccgaga caatcgccaa tatctacaca acccaacacc     4260

gcctcgatca aggagaaatt agcgcagggg acgctgccgt ggtgatgaca tcagcccaaa     4320

tcaccatggg aatgccctac gccgtcaccg atgctgtcct ggcaccacac attggcggag     4380

aggccgggtc aagtcatgca ccaccaccag ccctgactat ctttctcgac cggcatccaa     4440

ttgcattcat gctgtgctat cctgccgcac gctacctgat gggaagtatg acaccacagg     4500

ccgtcctcgc cttcgttgct ctgatccctc caaccctgcc tggcactaac atcgttctcg     4560

gcgcactccc cgaagacaga cacattgatc ggctggccaa gaggcaacgg cctggcgaga     4620

gactcgatct ggctatgctg gctgctatta ggagagtgta cgggctgctg gccaatactg     4680

tgagatacct ccaaggggga ggaagctggc gcgaggattg gggccaactg tctggcgctg     4740

ctgtgccacc tcaaggcgcc gagccacagt caaatgctgg tcctaggccc cacatcggcg     4800

atactctctt tacactgttc cgggcaccag agctgctcgc acctaatgga gatctgtaca     4860

atgttttcgc ttgggccctc gatgtcctgg ctaagcggct ccggcctatg cacgtgttca     4920

tcctcgacta cgaccagagc ccagctggtt gtcgggatgc tctcctgcaa ctgaccagcg     4980

ggatggtgca gacacacgtt actactcccg gctccatccc cactatctgt gacctcgccc     5040

ggacatttgc ccgggaaatg ggcgaagcca actgatcgtt ggggtatttg acaaactgac     5100

acgtatgggg tatttgacaa actgacatcg atggggtatt tgacaaactg acatcactgg     5160

ggtatttgac aaactgacag tcgacaactt gtttattgca gcttataatg gttacaaata     5220

aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg     5280

tttgtccaaa ctcatcaatg tatcttatca tgtctggatc ctatgcactg tcttagctgg     5340

tgttgatgaa catctgcacg atgtgcacga agctctgcag gaactccttg atgttcttct     5400

cctccagctc ctcgcactcc ttgcagccgc tctcggtcac gttgccgttg ctgctcaggc     5460

tgttgttggc caggatgatc aggtcctcca cggtgtcgtg gatgctggcg tcgccgctct     5520

ccaggctgat cacctgcagc tccagcagga agcacttcat ggcggtcacc ttgcagctgg     5580

ggtgcacgtc gctctcggtg tacagggtgg cgtcgatgtg catgctctgg atcaggtcct     5640

cgatcttctt caggtcgctg atcacgttca cccagttgcc tctggtggcg gggggtctca     5700

gcagcagcag cagcagcagg gcgggcaggc ccagggttct gcagcctctg gctcttctgg     5760

gggccatggt ggcagctctg cttatataga cctcccaccg tacacgccta ccgcccattt     5820

gcgtcaatgg ggcggagttg ttacgacatt ttggaaagtc ccgttgattt tggtgccaaa     5880

acaaactccc attgacgtca atggggtgga gacttggaaa tccccgtgag tcaaaccgct     5940

atccacgccc attgatgtac tgccaaaacc gcatcaccat ggtaatagcg atgactaata     6000

cgtagatgta ctgccaagta ggaaagtccc ataaggtcat gtactgggca taatgccagg     6060

cgggccattt accgtcattg acgtcaatag ggggcgtact tggcatatga tacacttgat     6120

gtactgccaa gtgggcagtt taccgtaaat actccaccca ttgacgtcaa tggaaagtcc     6180

ctattggcgt tactatggga acatacgtca ttattgacgt caatgggcgg gggtcgttgg     6240

gcggtcagcc aggcgggcca tttaccgtaa gttatgtaac gcggaactcc atatatgggc     6300

tatgaactaa tgaccccgta attgattact attaataact agtcaataat caatgtcaag     6360

cttggatcca tcgataaaat aaaagatttt atttagtctc cagaaaaagg ggggaatgaa     6420

agaccccacc tgtaggtttg gcaagctagc ttaagtaacg ccattttgca aggcatggaa     6480

aaatacataa ctgagaatag agaagttcag atcaaggtca ggaacagatg gaacagctga     6540

atatgggcca aacaggatat ctgtggtaag cagttcctgc cccggctcag ggccaagaac     6600

agatggaaca gctgaatatg ggccaaacag gatatctgtg gtaagcagtt cctgccccgg     6660

ctcagggcca agaacagatg gtccccagat gcggtccagc cctcagcagt ttctagagaa     6720

ccatcagatg tttccagggt gccccaagga cctgaaatga ccctgtgcct tatttgaact     6780

aaccaatcag ttcgcttctc gcttctgttc gcgcgcttct gctccccgag ctcaataaaa     6840

gagcccacaa cccctcactc ggcgcgccag tcctccgatt gactgagtcg cccgggtacc     6900

cgtgtatcca ataaaccctc ttgcagttgc atccgacttg tggtctcgct gttccttggg     6960

agggtctcct ctgagtgatt gactacccgt cagcgggggt ctttcatttg aagccgaatt     7020

cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca     7080

acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca     7140

cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc     7200

attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt     7260

cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact     7320

caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag     7380

caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata     7440

ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc     7500

cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg     7560

ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc     7620

tttctcaaag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg     7680

gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc     7740

ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga     7800

ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg     7860

gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa     7920

aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg     7980

tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt     8040

ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat     8100

tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct     8160

aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta     8220

tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa     8280

ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac     8340

gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa     8400

gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag     8460

taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg     8520

tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag     8580

ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg     8640

tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc     8700

ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat     8760

tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata     8820

ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa     8880

aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca     8940

actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc     9000

aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc     9060

tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg     9120

aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac     9180

ctgacgtcta agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga     9240

ggccctttcg tc                                                         9252


<210>  16
<211>  7261
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Construct 40: pBA-9b mCD19 (1D3)-IRES yCD

<400>  16
tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca       60

cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg      120

ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc      180

accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc      240

attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat      300

tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt      360

tttcccagtc acgacgttgt aaaacgacgg ccagtgccaa gctgactcta gaggatcgat      420

ccccggccgc tctagcttaa gtaacgccat tttgcaaggc atggaaaaat acataactga      480

gaatagagaa gttcagatca aggtcaggaa cagatggaac agctgaatat gggccaaaca      540

ggatatctgt ggtaagcagt tcctgccccg gctcagggcc aagaacagat ggaacagctg      600

aatatgggcc aaacaggata tctgtggtaa gcagttcctg ccccggctca gggccaagaa      660

cagatggtcc ccagatgcgg tccagccctc agcagtttct agagaaccat cagatgtttc      720

cagggtgccc caaggacctg aaatgaccct gtgccttatt tgaactaacc aatcagttcg      780

cttctcgctt ctgttcgcgc gcttctgctc cccgagctca ataaaagagc ccacaacccc      840

tcactcggcg cgccagtcct ccgattgact gagtcgcccg ggtacccgtg tatccaataa      900

accctcttgc agttgcatcc gacttgtggt ctcgctgttc cttgggaggg tctcctctga      960

gtgattgact acccgtcagc gggggtcttt catttggggg ctcgtccggg atcgggagac     1020

ccctgcccag ggaccaccga cccaccaccg ggaggtaagc tggccagcaa cttatctgtg     1080

tctgtccgat tgtctagtgt ctatgactga ttttatgcgc ctgcgtcggt actagttagc     1140

taactagctc tgtatctggc ggacccgtgg tggaactgac gagttcggaa cacccggccg     1200

caaccctggg agacgtccca gggacttcgg gggccgtttt tgtggcccga cctgagtcca     1260

aaaatcccga tcgttttgga ctctttggtg cacccccctt agaggaggga tatgtggttc     1320

tggtaggaga cgagaaccta aaacagttcc cgcctccgtc tgaatttttg ctttcggttt     1380

gggaccgaag ccgcgccgcg cgtcttgtct gctgcagcat cgttctgtgt tgtctctgtc     1440

tgactgtgtt tctgtatttg tctgagaatt aaggccagac tgttaccact ccctgaagtt     1500

tgaccttagg tcactggaaa gatgtcgagc ggatcgctca caaccagtcg gtagatgtca     1560

agaagagacg ttgggttacc ttctgctctg cagaatggcc aacctttaac gtcggatggc     1620

cgcgagacgg cacctttaac cgagacctca tcacccaggt taagatcaag gtcttttcac     1680

ctggcccgca tggacaccca gaccaggtcc cctacatcgt gacctgggaa gccttggctt     1740

ttgacccccc tccctgggtc aagccctttg tacaccctaa gcctccgcct cctcttcctc     1800

catccgcccc gtctctcccc cttgaacctc ctcgttcgac cccgcctcga tcctcccttt     1860

atccagccct cactccttct ctaggcgccg gaattaattc tcgaggggcc cagatctgcg     1920

gccgcatggg cgtgcccacc cagctgctgg gcctgctgct gctgtggatc accgacgcca     1980

tctgcgacat ccagatgacc cagagccctg ccagcctgag caccagcctg ggcgagaccg     2040

tgaccatcca gtgccaggcc agcgaggaca tctacagcgg cctggcctgg taccagcaga     2100

agcctggcaa gagccctcag ctgctgatct acggcgccag cgacctgcag gacggcgtgc     2160

ctagcaggtt cagcggcagc ggcagcggca cccagtacag cctgaagatc accagcatgc     2220

agaccgagga cgagggcgtg tacttctgcc agcagggcct gacctaccct aggaccttcg     2280

gcggcggcac caagctggag ctgaagggcg gcggcggcag cggcggcggc ggcagcggcg     2340

gcggcggcag cgaggtgcag ctgcagcaga gcggcgccga gctggtgagg cctggcacca     2400

gcgtgaagct gagctgcaag gtgagcggcg acaccatcac cttctactac atgcacttcg     2460

tgaagcagag gcctggccag ggcctggagt ggatcggcag gatcgaccct gaggacgaga     2520

gcaccaagta cagcgagaag ttcaagaaca aggccaccct gaccgccgac accagcagca     2580

acaccgccta cctgaagctg agcagcctga ccagcgagga caccgccacc tacttctgca     2640

tctacggcgg ctactacttc gactactggg gccagggcgt gatggtgacc gtgagcagca     2700

ttgagttcat gtaccctccg ccttacctag acaacgagag gagcaatgga actattattc     2760

acataaaaga gaaacatctt tgtcatactc agtcatctcc taagctgttt tgggcactgg     2820

tcgtggttgc tggagtcctg ttttgttatg gcttgctagt gacagtggct ctttgtgtta     2880

tctggacaaa atggatcagg aaaaaattcc cccacatatt caagcaacca tttaagaaga     2940

ccactggagc agctcaagag gaagatgctt gtagctgccg atgtccacag gaagaagaag     3000

gaggaggagg aggctatgag ctgagagcaa aattcagcag gagtgcagag actgctgcca     3060

acctgcagga ccccaaccag ctctacaatg agctcaatct agggcgaaga gaggaatatg     3120

acgtcttgga gaagaagcgg gctcgggatc cagagatggg aggcaaacag cagaggagga     3180

ggaaccccca ggaaggcgta tacaatgcac tgcagaaaga caagatggca gaagcctaca     3240

gtgagatcgg cacaaaaggc gagaggcgga gaggcaaggg gcacgatggc ctttaccagg     3300

gtctcagcac tgccaccaag gacacctatg atgccctgca tatgcagacc ctggcccctc     3360

gctgagtcga cacgcgttac tggccgaagc cgcttggaat aaggccggtg tgcgtttgtc     3420

tatatgttat tttccaccat attgccgtct tttggcaatg tgagggcccg gaaacctggc     3480

cctgtcttct tgacgagcat tcctaggggt ctttcccctc tcgccaaagg aatgcaaggt     3540

ctgttgaatg tcgtgaagga agcagttcct ctggaagctt cttgaagaca aacaacgtct     3600

gtagcgaccc tttgcaggca gcggaacccc ccacctggcg acaggtgcct ctgcggccaa     3660

aagccacgtg tataagatac acctgcaaag gcggcacaac cccagtgcca cgttgtgagt     3720

tggatagttg tggaaagagt caaatggctc tcctcaagcg tattcaacaa ggggctgaag     3780

gatgcccaga aggtacccca ttgtatggga tctgatctgg ggcctcggtg cacatgcttt     3840

acatgtgttt agtcgaggtt aaaaaacgtc taggcccccc gaaccacggg gacgtggttt     3900

tcctttgaaa aacacgatta taaatggtga ccggcggcat ggcctccaag tgggatcaaa     3960

agggcatgga tatcgcttac gaggaggccc tgctgggcta caaggagggc ggcgtgccta     4020

tcggcggctg tctgatcaac aacaaggacg gcagtgtgct gggcaggggc cacaacatga     4080

ggttccagaa gggctccgcc accctgcacg gcgagatctc caccctggag aactgtggca     4140

ggctggaggg caaggtgtac aaggacacca ccctgtacac caccctgtcc ccttgtgaca     4200

tgtgtaccgg cgctatcatc atgtacggca tccctaggtg tgtgatcggc gagaacgtga     4260

acttcaagtc caagggcgag aagtacctgc aaaccagggg ccacgaggtg gtggttgttg     4320

acgatgagag gtgtaagaag ctgatgaagc agttcatcga cgagaggcct caggactggt     4380

tcgaggatat cggcgagtaa tgcgctctcc agaaaaaggg gggaatgaaa gaccccacct     4440

gtaggtttgg caagctagct taagtaacgc cattttgcaa ggcatggaaa aatacataac     4500

tgagaataga gaagttcaga tcaaggtcag gaacagatgg aacagctgaa tatgggccaa     4560

acaggatatc tgtggtaagc agttcctgcc ccggctcagg gccaagaaca gatggaacag     4620

ctgaatatgg gccaaacagg atatctgtgg taagcagttc ctgccccggc tcagggccaa     4680

gaacagatgg tccccagatg cggtccagcc ctcagcagtt tctagagaac catcagatgt     4740

ttccagggtg ccccaaggac ctgaaatgac cctgtgcctt atttgaacta accaatcagt     4800

tcgcttctcg cttctgttcg cgcgcttctg ctccccgagc tcaataaaag agcccacaac     4860

ccctcactcg gcgcgccagt cctccgattg actgagtcgc ccgggtaccc gtgtatccaa     4920

taaaccctct tgcagttgca tccgacttgt ggtctcgctg ttccttggga gggtctcctc     4980

tgagtgattg actacccgtc agcgggggtc tttcatttga agccgaattc gtaatcatgg     5040

tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc     5100

ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg     5160

ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc     5220

ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact     5280

gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta     5340

atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag     5400

caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc     5460

cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta     5520

taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg     5580

ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcaaagc     5640

tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac     5700

gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac     5760

ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg     5820

aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga     5880

agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt     5940

agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag     6000

cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct     6060

gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg     6120

atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat     6180

gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc     6240

tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg     6300

gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct     6360

ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca     6420

actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg     6480

ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg     6540

tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc     6600

cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag     6660

ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg     6720

ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag     6780

tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat     6840

agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg     6900

atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca     6960

gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca     7020

aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat     7080

tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag     7140

aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa     7200

gaaaccatta ttatcatgac attaacctat aaaaataggc gtatcacgag gccctttcgt     7260

c                                                                     7261


<210>  17
<211>  4818
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Construct 50: hCD19CAR-IRES-yCD2_miRT2x20a-2x30d-223

<400>  17
ctagcttaag taacgccatt ttgcaaggca tggaaaaata cataactgag aatagagaag       60

ttcagatcaa ggtcaggaac agatggaaca gctgaatatg ggccaaacag gatatctgtg      120

gtaagcagtt cctgccccgg ctcagggcca agaacagatg gaacagctga atatgggcca      180

aacaggatat ctgtggtaag cagttcctgc cccggctcag ggccaagaac agatggtccc      240

cagatgcggt ccagccctca gcagtttcta gagaaccatc agatgtttcc agggtgcccc      300

aaggacctga aatgaccctg tgccttattt gaactaacca atcagttcgc ttctcgcttc      360

tgttcgcgcg cttctgctcc ccgagctcaa taaaagagcc cacaacccct cactcggcgc      420

gccagtcctc cgattgactg agtcgcccgg gtacccgtgt atccaataaa ccctcttgca      480

gttgcatccg acttgtggtc tcgctgttcc ttgggagggt ctcctctgag tgattgacta      540

cccgtcagcg ggggtctttc atttgggggc tcgtccggga tcgggagacc cctgcccagg      600

gaccaccgac ccaccaccgg gaggtaagct ggccagcaac ttatctgtgt ctgtccgatt      660

gtctagtgtc tatgactgat tttatgcgcc tgcgtcggta ctagttagct aactagctct      720

gtatctggcg gacccgtggt ggaactgacg agttcggaac acccggccgc aaccctggga      780

gacgtcccag ggacttcggg ggccgttttt gtggcccgac ctgagtccaa aaatcccgat      840

cgttttggac tctttggtgc acccccctta gaggagggat atgtggttct ggtaggagac      900

gagaacctaa aacagttccc gcctccgtct gaatttttgc tttcggtttg ggaccgaagc      960

cgcgccgcgc gtcttgtctg ctgcagcatc gttctgtgtt gtctctgtct gactgtgttt     1020

ctgtatttgt ctgagaatta aggccagact gttaccactc cctgaagttt gaccttaggt     1080

cactggaaag atgtcgagcg gatcgctcac aaccagtcgg tagatgtcaa gaagagacgt     1140

tgggttacct tctgctctgc agaatggcca acctttaacg tcggatggcc gcgagacggc     1200

acctttaacc gagacctcat cacccaggtt aagatcaagg tcttttcacc tggcccgcat     1260

ggacacccag accaggtccc ctacatcgtg acctgggaag ccttggcttt tgacccccct     1320

ccctgggtca agccctttgt acaccctaag cctccgcctc ctcttcctcc atccgccccg     1380

tctctccccc ttgaacctcc tcgttcgacc ccgcctcgat cctcccttta tccagccctc     1440

actccttctc taggcgccgg aattaattct cgaggggccc agatctgcgg ccgcatggcc     1500

ttaccagtga ccgccttgct cctgccgctg gccttgctgc tccacgccgc caggccggac     1560

atccagatga cacagactac atcctccctg tctgcctctc tgggagacag agtcaccatc     1620

agttgcaggg caagtcagga cattagtaaa tatttaaatt ggtatcagca gaaaccagat     1680

ggaactgtta aactcctgat ctaccataca tcaagattac actcaggagt cccatcaagg     1740

ttcagtggca gtgggtctgg aacagattat tctctcacca ttagcaacct ggagcaagaa     1800

gatattgcca cttacttttg ccaacagggt aatacgcttc cgtacacgtt cggagggggg     1860

accaagctgg agatcacagg tggcggtggc tcgggcggtg gtgggtcggg tggcggcgga     1920

tctgaggtga aactgcagga gtcaggacct ggcctggtgg cgccctcaca gagcctgtcc     1980

gtcacatgca ctgtctcagg ggtctcatta cccgactatg gtgtaagctg gattcgccag     2040

cctccacgaa agggtctgga gtggctggga gtaatatggg gtagtgaaac cacatactat     2100

aattcagctc tcaaatccag actgaccatc atcaaggaca actccaagag ccaagttttc     2160

ttaaaaatga acagtctgca aactgatgac acagccattt actactgtgc caaacattat     2220

tactacggtg gtagctatgc tatggactac tggggccaag gaacctcagt caccgtctcc     2280

tcaaccacga cgccagcgcc gcgaccacca acaccggcgc ccaccatcgc gtcgcagccc     2340

ctgtccctgc gcccagaggc gtgccggcca gcggcggggg gcgcagtgca cacgaggggg     2400

ctggacttcg cctgtgatat ctacatctgg gcgcccttgg ccgggacttg tggggtcctt     2460

ctcctgtcac tggttatcac cctttactgc aaacggggca gaaagaaact cctgtatata     2520

ttcaaacaac catttatgag accagtacaa actactcaag aggaagatgg ctgtagctgc     2580

cgatttccag aagaagaaga aggaggatgt gaactgagag tgaagttcag caggagcgca     2640

gacgcccccg cgtacaagca gggccagaac cagctctata acgagctcaa tctaggacga     2700

agagaggagt acgatgtttt ggacaagaga cgtggccggg accctgagat ggggggaaag     2760

ccgagaagga agaaccctca ggaaggcctg tacaatgaac tgcagaaaga taagatggcg     2820

gaggcctaca gtgagattgg gatgaaaggc gagcgccgga ggggcaaggg gcacgatggc     2880

ctttaccagg gtctcagtac agccaccaag gacacctacg acgcccttca catgcaggcc     2940

ctgccccctc gctaggtcga cacgcgttac tggccgaagc cgcttggaat aaggccggtg     3000

tgcgtttgtc tatatgttat tttccaccat attgccgtct tttggcaatg tgagggcccg     3060

gaaacctggc cctgtcttct tgacgagcat tcctaggggt ctttcccctc tcgccaaagg     3120

aatgcaaggt ctgttgaatg tcgtgaagga agcagttcct ctggaagctt cttgaagaca     3180

aacaacgtct gtagcgaccc tttgcaggca gcggaacccc ccacctggcg acaggtgcct     3240

ctgcggccaa aagccacgtg tataagatac acctgcaaag gcggcacaac cccagtgcca     3300

cgttgtgagt tggatagttg tggaaagagt caaatggctc tcctcaagcg tattcaacaa     3360

ggggctgaag gatgcccaga aggtacccca ttgtatggga tctgatctgg ggcctcggtg     3420

cacatgcttt acatgtgttt agtcgaggtt aaaaaacgtc taggcccccc gaaccacggg     3480

gacgtggttt tcctttgaaa aacacgatta taaatggtga ccggcggcat ggcctccaag     3540

tgggatcaaa agggcatgga tatcgcttac gaggaggccc tgctgggcta caaggagggc     3600

ggcgtgccta tcggcggctg tctgatcaac aacaaggacg gcagtgtgct gggcaggggc     3660

cacaacatga ggttccagaa gggctccgcc accctgcacg gcgagatctc caccctggag     3720

aactgtggca ggctggaggg caaggtgtac aaggacacca ccctgtacac caccctgtcc     3780

ccttgtgaca tgtgtaccgg cgctatcatc atgtacggca tccctaggtg tgtgatcggc     3840

gagaacgtga acttcaagtc caagggcgag aagtacctgc aaaccagggg ccacgaggtg     3900

gtggttgttg acgatgagag gtgtaagaag ctgatgaagc agttcatcga cgagaggcct     3960

caggactggt tcgaggatat cggcgagtaa tcgtactgca ttatgagcac ttaaaggcta     4020

tgtaaacatc cccgactgga agtacgtaaa gtgcttatag tgcaggtagt acgtgtaaac     4080

atccccgact ggaagattat ggggtatttg acaaactgac acgtatgggg tatttgacaa     4140

actgacatcg atggggtatt tgacaaactg acatcactgg ggtatttgac aaactgacag     4200

tcgactctcc agaaaaaggg gggaatgaaa gaccccacct gtaggtttgg caagctagct     4260

taagtaacgc cattttgcaa ggcatggaaa aatacataac tgagaataga gaagttcaga     4320

tcaaggtcag gaacagatgg aacagctgaa tatgggccaa acaggatatc tgtggtaagc     4380

agttcctgcc ccggctcagg gccaagaaca gatggaacag ctgaatatgg gccaaacagg     4440

atatctgtgg taagcagttc ctgccccggc tcagggccaa gaacagatgg tccccagatg     4500

cggtccagcc ctcagcagtt tctagagaac catcagatgt ttccagggtg ccccaaggac     4560

ctgaaatgac cctgtgcctt atttgaacta accaatcagt tcgcttctcg cttctgttcg     4620

cgcgcttctg ctccccgagc tcaataaaag agcccacaac ccctcactcg gcgcgccagt     4680

cctccgattg actgagtcgc ccgggtaccc gtgtatccaa taaaccctct tgcagttgca     4740

tccgacttgt ggtctcgctg ttccttggga gggtctcctc tgagtgattg actacccgtc     4800

agcgggggtc tttcattt                                                   4818


<210>  18
<211>  9386
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Construct 27: pSIN-BA-9b-EF1-hCD19CAR-IRES-CD2-WPRE-CMVpromIL15

<400>  18
tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca       60

cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg      120

ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc      180

accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc      240

attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat      300

tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt      360

tttcccagtc acgacgttgt aaaacgacgg ccagtgccaa gctgactcta gaggatcgat      420

ccccggccgc ttagttatta atagtaatca attacggggt cattagttca tagcccatat      480

atggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc gcccaacgac      540

ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat agggactttc      600

cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt acatcaagtg      660

tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc cgcctggcat      720

tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta cgtattagtc      780

atcgctatta ccatggtgat gcggttttgg cagtacatca atgggcgtgg atagcggttt      840

gactcacggg gatttccaag tctccacccc attgacgtca atgggagttt gttttggcac      900

caaaatcaac gggactttcc aaaatgtcgt aacaactccg ccccattgac gcaaatgggc      960

ggtaggcgtg tacggtggga ggtctatata agcagagctg gtttagtgaa ccggcgccag     1020

tcctccgatt gactgagtcg cccgggtacc cgtgtatcca ataaaccctc ttgcagttgc     1080

atccgacttg tggtctcgct gttccttggg agggtctcct ctgagtgatt gactacccgt     1140

cagcgggggt ctttcatttg ggggctcgtc cgggatcggg agacccctgc ccagggacca     1200

ccgacccacc accgggaggt aagctggcca gcaacttatc tgtgtctgtc cgattgtcta     1260

gtgtctatga ctgattttat gcgcctgcgt cggtactagt tagctaacta gctctgtatc     1320

tggcggaccc gtggtggaac tgacgagttc ggaacacccg gccgcaaccc tgggagacgt     1380

cccagggact tcgggggccg tttttgtggc ccgacctgag tccaaaaatc ccgatcgttt     1440

tggactcttt ggtgcacccc ccttagagga gggatatgtg gttctggtag gagacgagaa     1500

cctaaaacag ttcccgcctc cgtctgaatt tttgctttcg gtttgggacc gaagccgcgc     1560

cgcgcgtctt gtctgctgca gcatcgttct gtgttgtctc tgtctgactg tgtttctgta     1620

tttgtctgag aattaaggcc agactgttac cactccctga agtttgacct taggtcactg     1680

gaaagatgtc gagcggatcg ctcacaacca gtcggtagat gtcaagaaga gacgttgggt     1740

taccttctgc tctgcagaat ggccaacctt taacgtcgga tggccgcgag acggcacctt     1800

taaccgagac ctcatcaccc aggttaagat caaggtcttt tcacctggcc cgcatggaca     1860

cccagaccag gtcccctaca tcgtgacctg ggaagccttg gcttttgacc cccctccctg     1920

ggtcaagccc tttgtacacc ctaagcctcc gcctcctctt cctccatccg ccccgtctct     1980

cccccttgaa cctcctcgtt cgaccccgcc tcgatcctcc ctttatccag ccctcactcc     2040

ttctctaggc gccggaatta attctcgagg gatctgcgat cgctccggtg cccgtcagtg     2100

ggcagagcgc acatcgccca cagtccccga gaagttgggg ggaggggtcg gcaattgaac     2160

gggtgcctag agaaggtggc gcggggtaaa ctgggaaagt gatgtcgtgt actggctccg     2220

cctttttccc gagggtgggg gagaaccgta tataagtgca gtagtcgccg tgaacgttct     2280

ttttcgcaac gggtttgccg ccagaacaca gctgaagctt cgagggggcc catggcctta     2340

ccagtgaccg ccttgctcct gccgctggcc ttgctgctcc acgccgccag gccggacatc     2400

cagatgacac agactacatc ctccctgtct gcctctctgg gagacagagt caccatcagt     2460

tgcagggcaa gtcaggacat tagtaaatat ttaaattggt atcagcagaa accagatgga     2520

actgttaaac tcctgatcta ccatacatca agattacact caggagtccc atcaaggttc     2580

agtggcagtg ggtctggaac agattattct ctcaccatta gcaacctgga gcaagaagat     2640

attgccactt acttttgcca acagggtaat acgcttccgt acacgttcgg aggggggacc     2700

aagctggaga tcacaggtgg cggtggctcg ggcggtggtg ggtcgggtgg cggcggatct     2760

gaggtgaaac tgcaggagtc aggacctggc ctggtggcgc cctcacagag cctgtccgtc     2820

acatgcactg tctcaggggt ctcattaccc gactatggtg taagctggat tcgccagcct     2880

ccacgaaagg gtctggagtg gctgggagta atatggggta gtgaaaccac atactataat     2940

tcagctctca aatccagact gaccatcatc aaggacaact ccaagagcca agttttctta     3000

aaaatgaaca gtctgcaaac tgatgacaca gccatttact actgtgccaa acattattac     3060

tacggtggta gctatgctat ggactactgg ggccaaggaa cctcagtcac cgtctcctca     3120

accacgacgc cagcgccgcg accaccaaca ccggcgccca ccatcgcgtc gcagcccctg     3180

tccctgcgcc cagaggcgtg ccggccagcg gcggggggcg cagtgcacac gagggggctg     3240

gacttcgcct gtgatatcta catctgggcg cccttggccg ggacttgtgg ggtccttctc     3300

ctgtcactgg ttatcaccct ttactgcaaa cggggcagaa agaaactcct gtatatattc     3360

aaacaaccat ttatgagacc agtacaaact actcaagagg aagatggctg tagctgccga     3420

tttccagaag aagaagaagg aggatgtgaa ctgagagtga agttcagcag gagcgcagac     3480

gcccccgcgt acaagcaggg ccagaaccag ctctataacg agctcaatct aggacgaaga     3540

gaggagtacg atgttttgga caagagacgt ggccgggacc ctgagatggg gggaaagccg     3600

agaaggaaga accctcagga aggcctgtac aatgaactgc agaaagataa gatggcggag     3660

gcctacagtg agattgggat gaaaggcgag cgccggaggg gcaaggggca cgatggcctt     3720

taccagggtc tcagtacagc caccaaggac acctacgacg cccttcacat gcaggccctg     3780

ccccctcgct agatcgatac gcgttactgg ccgaagccgc ttggaataag gccggtgtgc     3840

gtttgtctat atgttatttt ccaccatatt gccgtctttt ggcaatgtga gggcccggaa     3900

acctggccct gtcttcttga cgagcattcc taggggtctt tcccctctcg ccaaaggaat     3960

gcaaggtctg ttgaatgtcg tgaaggaagc agttcctctg gaagcttctt gaagacaaac     4020

aacgtctgta gcgacccttt gcaggcagcg gaacccccca cctggcgaca ggtgcctctg     4080

cggccaaaag ccacgtgtat aagatacacc tgcaaaggcg gcacaacccc agtgccacgt     4140

tgtgagttgg atagttgtgg aaagagtcaa atggctctcc tcaagcgtat tcaacaaggg     4200

gctgaaggat gcccagaagg taccccattg tatgggatct gatctggggc ctcggtgcac     4260

atgctttaca tgtgtttagt cgaggttaaa aaacgtctag gccccccgaa ccacggggac     4320

gtggttttcc tttgaaaaac acgattataa atggtgaccg gcggcatggc ctccaagtgg     4380

gatcaaaagg gcatggatat cgcttacgag gaggccctgc tgggctacaa ggagggcggc     4440

gtgcctatcg gcggctgtct gatcaacaac aaggacggca gtgtgctggg caggggccac     4500

aacatgaggt tccagaaggg ctccgccacc ctgcacggcg agatctccac cctggagaac     4560

tgtggcaggc tggagggcaa ggtgtacaag gacaccaccc tgtacaccac cctgtcccct     4620

tgtgacatgt gtaccggcgc tatcatcatg tacggcatcc ctaggtgtgt gatcggcgag     4680

aacgtgaact tcaagtccaa gggcgagaag tacctgcaaa ccaggggcca cgaggtggtg     4740

gttgttgacg atgagaggtg taagaagctg atgaagcagt tcatcgacga gaggcctcag     4800

gactggttcg aggatatcgg cgagtaacaa tcaacctctg gattacaaaa tttgtgaaag     4860

attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat     4920

gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc     4980

ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg     5040

cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct     5100

ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct     5160

tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg     5220

gaaatcatcg tcctttcctt ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac     5280

gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct     5340

gccggctctg cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct     5400

ttgggccgcc tccccgcctg aatacgagct cgacattgat tattgactag ttattaatag     5460

taatcaatta cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt     5520

acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg     5580

acgtatgttc ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat     5640

ttacggtaaa ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct     5700

attgacgtca atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg     5760

gactttccta cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg     5820

ttttggcagt acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc     5880

caccccattg acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa     5940

tgtcgtaaca actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc     6000

tatataagca gagctgccac catggccccc agaagagcca gaggctgcag aaccctgggc     6060

ctgcccgccc tgctgctgct gctgctgctg agaccccccg ccaccagagg caactgggtg     6120

aacgtgatca gcgacctgaa gaagatcgag gacctgatcc agagcatgca catcgacgcc     6180

accctgtaca ccgagagcga cgtgcacccc agctgcaagg tgaccgccat gaagtgcttc     6240

ctgctggagc tgcaggtgat cagcctggag agcggcgacg ccagcatcca cgacaccgtg     6300

gaggacctga tcatcctggc caacaacagc ctgagcagca acggcaacgt gaccgagagc     6360

ggctgcaagg agtgcgagga gctggaggag aagaacatca aggagttcct gcagagcttc     6420

gtgcacatcg tgcagatgtt catcaacacc agctaagcgt cacgtaaaat aaaagatttt     6480

atttagtctc cagaaaaagg ggggaaaatg aaagacccca cctgtaggtt tggcaagcta     6540

gcttaagtaa cgccattttg caaggcatgg aaaaatacat aactgagaat agagaagttc     6600

agatcaaggt cagtccccag atgcggtcca gccctcagca gtttctagag aaccatcaga     6660

tgtttccagg gtgccccaag gacctgaaat gaccctgtgc cttatttgaa ctaaccaatc     6720

agttcgcttc tcgcttctgt tcgcgcgctt ctgctccccg agctcaataa aagagcccac     6780

aacccctcac tcggcgcgcc agtcctccga ttgactgagt cgcccgggta cccgtgtatc     6840

caataaaccc tcttgcagtt gcatccgact tgtggtctcg ctgttccttg ggagggtctc     6900

ctctgagtga ttgactaccc gtcagcgggg gtcttcattt gaaagacccc acctgtaggt     6960

ttggcaaaat aaaagagccc acaacccctc actcggcgcg ccagtcctcc gattgactga     7020

gtcgcccggg tacccgtgta tccaataaac cctcttgcag ttgcatccga cttgtggtct     7080

cgctgttcct tgggagggtc tcctctgagt gattgactac ccgtcagcgg gggtctttca     7140

tttgaagccg aattcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct     7200

cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg     7260

agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct     7320

gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg     7380

gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc     7440

ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg     7500

aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct     7560

ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca     7620

gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct     7680

cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc     7740

gggaagcgtg gcgctttctc aaagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt     7800

tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc     7860

cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc     7920

cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg     7980

gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc     8040

agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag     8100

cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga     8160

tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat     8220

tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag     8280

ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat     8340

cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc     8400

cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat     8460

accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag     8520

ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg     8580

ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc     8640

tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca     8700

acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg     8760

tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc     8820

actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta     8880

ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc     8940

aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg     9000

ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc     9060

cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc     9120

aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat     9180

actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag     9240

cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc     9300

ccgaaaagtg ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa     9360

taggcgtatc acgaggccct ttcgtc                                          9386


<210>  19
<211>  8974
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Construct 28: SIN lenti-hCD19-CAR-IRES-CD2-WPRE-CMV-IL15

<400>  19
aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca       60

tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga      120

tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt      180

gccgcattgc agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg      240

gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc      300

tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg      360

taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg      420

aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt      480

gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg      540

actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga      600

attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta      660

aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta      720

gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga      780

tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg      840

atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt      900

aagaccaccg cacagcaagc ggccggccgc tgatcttcag acctggagga ggagatatga      960

gggacaattg gagaagtgaa ttatataaat ataaagtagt aaaaattgaa ccattaggag     1020

tagcacccac caaggcaaag agaagagtgg tgcagagaga aaaaagagca gtgggaatag     1080

gagctttgtt ccttgggttc ttgggagcag caggaagcac tatgggcgca gcgtcaatga     1140

cgctgacggt acaggccaga caattattgt ctggtatagt gcagcagcag aacaatttgc     1200

tgagggctat tgaggcgcaa cagcatctgt tgcaactcac agtctggggc atcaagcagc     1260

tccaggcaag aatcctggct gtggaaagat acctaaagga tcaacagctc ctggggattt     1320

ggggttgctc tggaaaactc atttgcacca ctgctgtgcc ttggaatgct agttggagta     1380

ataaatctct ggaacagatt tggaatcaca cgacctggat ggagtgggac agagaaatta     1440

acaattacac aagcttaata cactccttaa ttgaagaatc gcaaaaccag caagaaaaga     1500

atgaacaaga attattggaa ttagataaat gggcaagttt gtggaattgg tttaacataa     1560

caaattggct gtggtatata aaattattca taatgatagt aggaggcttg gtaggtttaa     1620

gaatagtttt tgctgtactt tctatagtga atagagttag gcagggatat tcaccattat     1680

cgtttcagac ccacctccca accccgaggg gacccgacag gcccgaagga atagaagaag     1740

aaggtggaga gagagacaga gacagatcca ttcgattagt gaacggatct cgacggtatc     1800

ggttaacctt taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag     1860

acataatagc aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa     1920

attttcgggt ttattacagg gacagcagag atccagttta tcgataatga aagaccccac     1980

ctgtaggttt ggcaagctcg agggatctgc gatcgctccg gtgcccgtca gtgggcagag     2040

cgcacatcgc ccacagtccc cgagaagttg gggggagggg tcggcaattg aacgggtgcc     2100

tagagaaggt ggcgcggggt aaactgggaa agtgatgtcg tgtactggct ccgccttttt     2160

cccgagggtg ggggagaacc gtatataagt gcagtagtcg ccgtgaacgt tctttttcgc     2220

aacgggtttg ccgccagaac acagctgaag cttcgaggtt tatgaattca ccatggcctt     2280

accagtgacc gccttgctcc tgccgctggc cttgctgctc cacgccgcca ggccggacat     2340

ccagatgaca cagactacat cctccctgtc tgcctctctg ggagacagag tcaccatcag     2400

ttgcagggca agtcaggaca ttagtaaata tttaaattgg tatcagcaga aaccagatgg     2460

aactgttaaa ctcctgatct accatacatc aagattacac tcaggagtcc catcaaggtt     2520

cagtggcagt gggtctggaa cagattattc tctcaccatt agcaacctgg agcaagaaga     2580

tattgccact tacttttgcc aacagggtaa tacgcttccg tacacgttcg gaggggggac     2640

caagctggag atcacaggtg gcggtggctc gggcggtggt gggtcgggtg gcggcggatc     2700

tgaggtgaaa ctgcaggagt caggacctgg cctggtggcg ccctcacaga gcctgtccgt     2760

cacatgcact gtctcagggg tctcattacc cgactatggt gtaagctgga ttcgccagcc     2820

tccacgaaag ggtctggagt ggctgggagt aatatggggt agtgaaacca catactataa     2880

ttcagctctc aaatccagac tgaccatcat caaggacaac tccaagagcc aagttttctt     2940

aaaaatgaac agtctgcaaa ctgatgacac agccatttac tactgtgcca aacattatta     3000

ctacggtggt agctatgcta tggactactg gggccaagga acctcagtca ccgtctcctc     3060

aaccacgacg ccagcgccgc gaccaccaac accggcgccc accatcgcgt cgcagcccct     3120

gtccctgcgc ccagaggcgt gccggccagc ggcggggggc gcagtgcaca cgagggggct     3180

ggacttcgcc tgtgatatct acatctgggc gcccttggcc gggacttgtg gggtccttct     3240

cctgtcactg gttatcaccc tttactgcaa acggggcaga aagaaactcc tgtatatatt     3300

caaacaacca tttatgagac cagtacaaac tactcaagag gaagatggct gtagctgccg     3360

atttccagaa gaagaagaag gaggatgtga actgagagtg aagttcagca ggagcgcaga     3420

cgcccccgcg tacaagcagg gccagaacca gctctataac gagctcaatc taggacgaag     3480

agaggagtac gatgttttgg acaagagacg tggccgggac cctgagatgg ggggaaagcc     3540

gagaaggaag aaccctcagg aaggcctgta caatgaactg cagaaagata agatggcgga     3600

ggcctacagt gagattggga tgaaaggcga gcgccggagg ggcaaggggc acgatggcct     3660

ttaccagggt ctcagtacag ccaccaagga cacctacgac gcccttcaca tgcaggccct     3720

gccccctcgc tagatcgata cgcgttactg gccgaagccg cttggaataa ggccggtgtg     3780

cgtttgtcta tatgttattt tccaccatat tgccgtcttt tggcaatgtg agggcccgga     3840

aacctggccc tgtcttcttg acgagcattc ctaggggtct ttcccctctc gccaaaggaa     3900

tgcaaggtct gttgaatgtc gtgaaggaag cagttcctct ggaagcttct tgaagacaaa     3960

caacgtctgt agcgaccctt tgcaggcagc ggaacccccc acctggcgac aggtgcctct     4020

gcggccaaaa gccacgtgta taagatacac ctgcaaaggc ggcacaaccc cagtgccacg     4080

ttgtgagttg gatagttgtg gaaagagtca aatggctctc ctcaagcgta ttcaacaagg     4140

ggctgaagga tgcccagaag gtaccccatt gtatgggatc tgatctgggg cctcggtgca     4200

catgctttac atgtgtttag tcgaggttaa aaaacgtcta ggccccccga accacgggga     4260

cgtggttttc ctttgaaaaa cacgattata aatggtgacc ggcggcatgg cctccaagtg     4320

ggatcaaaag ggcatggata tcgcttacga ggaggccctg ctgggctaca aggagggcgg     4380

cgtgcctatc ggcggctgtc tgatcaacaa caaggacggc agtgtgctgg gcaggggcca     4440

caacatgagg ttccagaagg gctccgccac cctgcacggc gagatctcca ccctggagaa     4500

ctgtggcagg ctggagggca aggtgtacaa ggacaccacc ctgtacacca ccctgtcccc     4560

ttgtgacatg tgtaccggcg ctatcatcat gtacggcatc cctaggtgtg tgatcggcga     4620

gaacgtgaac ttcaagtcca agggcgagaa gtacctgcaa accaggggcc acgaggtggt     4680

ggttgttgac gatgagaggt gtaagaagct gatgaagcag ttcatcgacg agaggcctca     4740

ggactggttc gaggatatcg gcgagtaaca atcaacctct ggattacaaa atttgtgaaa     4800

gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa     4860

tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat     4920

cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt     4980

gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc     5040

tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc     5100

ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg     5160

ggaaatcatc gtcctttcct tggctgctcg cctgtgttgc cacctggatt ctgcgcggga     5220

cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc     5280

tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc     5340

tttgggccgc ctccccgcct gaatacaact tgtttattgc agcttataat ggttacaaat     5400

aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg     5460

gtttgtccaa actcatcaat gtatcttatc atgtctggat cgagctcgac attgattatt     5520

gactagttat taatagtaat caattacggg gtcattagtt catagcccat atatggagtt     5580

ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc     5640

attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg     5700

tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat     5760

gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca     5820

gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat     5880

taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt ttgactcacg     5940

gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc accaaaatca     6000

acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg gcggtaggcg     6060

tgtacggtgg gaggtctata taagcagagc tgccaccatg gcccccagaa gagccagagg     6120

ctgcagaacc ctgggcctgc ccgccctgct gctgctgctg ctgctgagac cccccgccac     6180

cagaggcaac tgggtgaacg tgatcagcga cctgaagaag atcgaggacc tgatccagag     6240

catgcacatc gacgccaccc tgtacaccga gagcgacgtg caccccagct gcaaggtgac     6300

cgccatgaag tgcttcctgc tggagctgca ggtgatcagc ctggagagcg gcgacgccag     6360

catccacgac accgtggagg acctgatcat cctggccaac aacagcctga gcagcaacgg     6420

caacgtgacc gagagcggct gcaaggagtg cgaggagctg gaggagaaga acatcaagga     6480

gttcctgcag agcttcgtgc acatcgtgca gatgttcatc aacaccagcc tcgagactag     6540

ttctagagga tccgcggccg ccgcccctct ccctcccccc cccctaacgt tactggccga     6600

agccgcttgg aataaggccg gtgtgcgttt gtctatatgt tattttccac catattgccg     6660

tcttttggca atgtgagggc ccggaaacct ggccctgtct tcttgacgag cattcctagg     6720

ggtctttccc ctctcgccaa aggaatgcaa ggtctgttga atgtcgtgaa ggaagcagtt     6780

cctctggaag cttcttgaag acaaacaacg tctgtagcga ccctttgcag gcagcggaac     6840

cccccacctg gcgacaggtg cctctgcggc caaaagccac gtgtataaga tacacctgca     6900

aaggcggcac aaccccagtg ccacgttgtg agttggatag ttgtggaaag agtcaaatgg     6960

ctctcctcaa gcgtattcaa caaggggctg aaggatgccc agaaggtacc ccattgtatg     7020

ggatctgatc tggggcctcg gtgcacatgc tttacatgtg tttagtcgag gttaaaaaaa     7080

cgtctaggcc ccccgaacca cggggacgtg gttttccttt gaaaaacacg atacgtaatg     7140

accgagtaca agcccacggt gcgcctcgcc acccgcgacg acgtcccccg ggccgtacgc     7200

accctcgccg ccgcgttcgc cgactacccc gccacgcgcc acaccgtcga cccggaccgc     7260

cacatcgagc gggtcaccga gctgcaagaa ctcttcctca cgcgcgtcgg gctcgacatc     7320

ggcaaggtgt gggtcgcgga cgacggcgcc gcggtggcgg tctggaccac gccggagagc     7380

gtcgaagcgg gggcggtgtt cgccgagatc ggcccgcgca tggccgagtt gagcggttcc     7440

cggctggccg cgcagcaaca gatggaaggc ctcctggcgc cgcaccggcc caaggagccc     7500

gcgtggttcc tggccaccgt cggcgtctcg cccgaccacc agggcaaggg tctgggcagc     7560

gccgtcgtgc tccccggagt ggaggcggcc gagcgcgccg gggtgcccgc cttcctggag     7620

acctccgcgc cccgcaacct ccccttctac gagcggctcg gcttcaccgt caccgccgac     7680

gtcgaggtgc ccgaaggacc gcgcacctgg tgcatgaccc gcaagcccgg tgcctagacg     7740

cgtctggaac aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa     7800

ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat     7860

tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta     7920

tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc     7980

aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt     8040

ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg     8100

ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaaatcat cgtcctttcc     8160

ttggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc     8220

ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct     8280

tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgcc     8340

tgaatacgag ctcggtacct ttaagaccaa tgacttacaa ggcagctgta gatcttagcc     8400

actttttaaa agaaaagagg ggactggaag ggctaattca ctcccaacga agacaagatc     8460

tgctttttgc ctgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg     8520

gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag     8580

tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag     8640

tgtggaaaat ctctagcagt agtagttcat gtcatcttat tattcagtat ttataacttg     8700

caaagaaatg aatatcagag agtgagagga acttgtttat tgcagcttat aatggttaca     8760

aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt     8820

gtggtttgtc caaactcatc aatgtatctt atcatgtctg gctctagcta tcccgcccct     8880

aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg     8940

actaattttt tttatttatg cagaggccga ggcc                                 8974


<210>  20
<211>  5527
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Construct 53: pCMVenvMtFDraLBGH

<400>  20
gctcgccttc tagttgccag ccatctgttg tttgcccctc ccccgtgcct tccttgaccc       60

tggaaggtgc cactcccact gtcctttcct aataaaatga ggaaattgca tcgcattgtc      120

tgagtaggtg tcattctatt ctggggggtg gggtggggca ggacagcaag ggggaggatt      180

gggaagacaa tagcaggcat gctggggatg cggtgggctc tatggcggcc gccaccgcgg      240

tggagctcca gcttttgttc cctttagtga gggttaattc cgagcttggc gtaatcatgg      300

tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc      360

ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg      420

ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc      480

ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact      540

gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta      600

atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag      660

caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc      720

cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta      780

taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg      840

ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc      900

tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac      960

gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac     1020

ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg     1080

aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga     1140

aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt     1200

agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag     1260

cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct     1320

gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg     1380

atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat     1440

gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc     1500

tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg     1560

gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct     1620

ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca     1680

actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg     1740

ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg     1800

tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc     1860

cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag     1920

ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg     1980

ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag     2040

tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat     2100

agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg     2160

atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca     2220

gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca     2280

aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat     2340

tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag     2400

aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgggacgcgc     2460

cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac     2520

ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg     2580

ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt     2640

tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc     2700

cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct     2760

tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga     2820

ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga     2880

attttaacaa aatattaacg tttacaattt cgcgccattc gccattcagg ctgcgcaact     2940

gttgggaagg gcgatcggtg cgggcctctt cgctattacg ccagctggcg aaagggggat     3000

gtgctgcaag gcgattaagt tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa     3060

cgacggccag tgaattgtaa tacgactcac tatagggcga attgggtacc gggccccccc     3120

tcgaggtcga cattgattat tgactagtta ttaatagtaa tcaattacgg ggtcattagt     3180

tcatagccca tatatggagt tccgcgttac ataacttacg gtaaatggcc cgcctggctg     3240

accgcccaac gacccccgcc cattgacgtc aataatgacg tatgttccca tagtaacgcc     3300

aatagggact ttccattgac gtcaatgggt ggagtattta cggtaaactg cccacttggc     3360

agtacatcaa gtgtatcata tgccaagtac gccccctatt gacgtcaatg acggtaaatg     3420

gcccgcctgg cattatgccc agtacatgac cttatgggac tttcctactt ggcagtacat     3480

ctacgtatta gtcatcgcta ttaccatggt gatgcggttt tggcagtaca tcaatgggcg     3540

tggatagcgg tttgactcac ggggatttcc aagtctccac cccattgacg tcaatgggag     3600

tttgttttgg caccaaaatc aacgggactt tccaaaatgt cgtaacaact ccgccccatt     3660

gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat ataagcagag ctcgtttagt     3720

gaaccgtcag atcgcctgga gacgccatcc acgctgtttt gacctccata gaagacaccg     3780

ggaccgatcc agcctccgcg gccgacggta tcgataagct tgatctagag actgggtgct     3840

cctactcccc ttagccctgt accgagcccg caacacgccg ggcccccatg gcctcacccc     3900

atatgagatc ttatatgggg cacccccgcc ccttgtaaac ttccctgacc ctgacatgtc     3960

catcatgggt ctcaaggtga acgtctctgc catattcatg gcagtactgt taactctcca     4020

aacacccacc ggtcaaatcc attggggcaa tctctctaag ataggggtgg taggaatagg     4080

aagtgcaagc tacaaagtta tgactcgttc cagccatcaa tcattagtca taaaattaat     4140

gcccaatata actctcctca ataactgcac gagggtagag attgcagaat acaggagact     4200

actgagaaca gttttggaac caattagaga tgcacttaat gcaatgaccc agaatataag     4260

accggttcag agtgtagctt caagtaggag acacaagaga tttgcgggag tagtcctggc     4320

aggtgcggcc ctaggcgttg ccacagctgc tcagataaca gccggcattg cacttcacca     4380

gtccatgctg aactctcaag ccatcgacaa tctgagagcg agcctggaaa ctactaatca     4440

ggcaattgag gcaatcagac aagcagggca ggagatgata ttggctgttc agggtgtcca     4500

agactacatc aataatgagc tgataccgtc tatgaaccaa ctatcttgtg atttaatcgg     4560

ccagaagctc gggctcaaat tgctcagata ctatacagaa atcctgtcat tatttggccc     4620

cagtttacgg gaccccatat ctgcggagat atctatccag gctttgagct atgcgcttgg     4680

aggagacatc aataaggtgt tagaaaagct cggatacagt ggaggtgatt tactgggcat     4740

cttagagagc ggaggaataa aggcccggat aactcacgtc gacacagagt cctacttcat     4800

tgtcctcagt atagcctatc cgacgctgtc cgagattaag ggggtgattg tccaccggct     4860

agagggggtc tcgtacaaca taggctctca agagtggtat accactgtgc ccaagtatgt     4920

tgcaacccaa gggtacctta tctcgaattt tgatgagtca tcgtgtactt tcatgccaga     4980

ggggactgtg tgcagccaaa atgccttgta cccgatgagt cctctgctcc aagaatgcct     5040

ccgggggtac accaagtcct gtgctcgtac actcgtatcc gggtcttttg ggaaccggtt     5100

cattttatca caagggaacc taatagccaa ttgtgcatca atcctttgca agtgttacac     5160

aacaggaacg atcattaatc aagaccctga caagatccta acatacattg ctgccgatca     5220

ctgcccggta gtcgaggtga acggcgtgac catccaagtc gggagcagga ggtatccaga     5280

cgctgtgtac ttgcacagaa ttgacctcgg tcctcccata tcattggaga ggttggacgt     5340

agggacaaat ctggggaatg caattgctaa gttggaggat gccaaggaat tgttggagtc     5400

atcggaccag atattgagga gtatgaaagg tttatcgagc actagcatag tctacatcct     5460

gattgcagtg tgtcttggag ggttgatagg gatccccgct ttaatatgtt gctgcagggg     5520

gcgttga                                                               5527


<210>  21
<211>  6847
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Construct 52: pCMVenvMFhlCD8DraLBGH


<220>
<221>  misc_feature
<222>  (4136)..(4136)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (4234)..(4234)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (4238)..(4239)
<223>  n is a, c, g, or t

<400>  21
gctcgccttc tagttgccag ccatctgttg tttgcccctc ccccgtgcct tccttgaccc       60

tggaaggtgc cactcccact gtcctttcct aataaaatga ggaaattgca tcgcattgtc      120

tgagtaggtg tcattctatt ctggggggtg gggtggggca ggacagcaag ggggaggatt      180

gggaagacaa tagcaggcat gctggggatg cggtgggctc tatggcggcc gccaccgcgg      240

tggagctcca gcttttgttc cctttagtga gggttaattc cgagcttggc gtaatcatgg      300

tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc      360

ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg      420

ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc      480

ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact      540

gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta      600

atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag      660

caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc      720

cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta      780

taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg      840

ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc      900

tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac      960

gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac     1020

ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg     1080

aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga     1140

aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt     1200

agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag     1260

cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct     1320

gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg     1380

atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat     1440

gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc     1500

tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg     1560

gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct     1620

ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca     1680

actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg     1740

ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg     1800

tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc     1860

cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag     1920

ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg     1980

ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag     2040

tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat     2100

agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg     2160

atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca     2220

gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca     2280

aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat     2340

tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag     2400

aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgggacgcgc     2460

cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac     2520

ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg     2580

ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt     2640

tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc     2700

cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct     2760

tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga     2820

ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga     2880

attttaacaa aatattaacg tttacaattt cgcgccattc gccattcagg ctgcgcaact     2940

gttgggaagg gcgatcggtg cgggcctctt cgctattacg ccagctggcg aaagggggat     3000

gtgctgcaag gcgattaagt tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa     3060

cgacggccag tgaattgtaa tacgactcac tatagggcga attgggtacc gggccccccc     3120

tcgaggtcga cattgattat tgactagtta ttaatagtaa tcaattacgg ggtcattagt     3180

tcatagccca tatatggagt tccgcgttac ataacttacg gtaaatggcc cgcctggctg     3240

accgcccaac gacccccgcc cattgacgtc aataatgacg tatgttccca tagtaacgcc     3300

aatagggact ttccattgac gtcaatgggt ggagtattta cggtaaactg cccacttggc     3360

agtacatcaa gtgtatcata tgccaagtac gccccctatt gacgtcaatg acggtaaatg     3420

gcccgcctgg cattatgccc agtacatgac cttatgggac tttcctactt ggcagtacat     3480

ctacgtatta gtcatcgcta ttaccatggt gatgcggttt tggcagtaca tcaatgggcg     3540

tggatagcgg tttgactcac ggggatttcc aagtctccac cccattgacg tcaatgggag     3600

tttgttttgg caccaaaatc aacgggactt tccaaaatgt cgtaacaact ccgccccatt     3660

gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat ataagcagag ctcgtttagt     3720

gaaccgtcag atcgcctgga gacgccatcc acgctgtttt gacctccata gaagacaccg     3780

ggaccgatcc agcctccgcg gccgacggta tcgataagct tgatctagag actgggtgct     3840

cctactcccc ttagccctgt accgagcccg caacacgccg ggcccccatg gcctcacccc     3900

atatgagatc ttatatgggg cacccccgcc ccttgtaaac ttccctgacc ctgacatgac     3960

cagagttact aacagcccct ctctccaagc tcacttacag gctctctact tagtccagca     4020

cgaagtttgg agaccactgg cggcagttac caagaacaac tggaccggcc ggtggtgctc     4080

acccttccgg gtcgcgacac agtgtgggtc gcgacatcaa cagactagac tgaagnaacc     4140

tagaacctcg ctggaaagga ccttacacag tcctgctgac cacccccacc gccctcaaag     4200

tagacggtat cgcagcttgg atacacgcag cccncgtnnc ggccgacacc cagagtggac     4260

catcctctgg acggacatgg gcagcagaat cgtgatcaac agagagcacc tgatgatcga     4320

cagaccctac gtgctgctgg ccgtgctgtt cgtgatgttc ctgagcctga tcggcctgct     4380

ggccatcgcc ggcatcagac tgcacagagc cgccatctac accgccgaga tccacaagag     4440

cctgagcacc aacctggacg tgaccaacag catcgagcac caggtgaagg acgtgctgac     4500

ccccctgttc aagatcatcg gcgacgaggt gggcctgaga accccccaga gattcaccga     4560

cctggtgaag ttcatcagcg acaagatcaa gttcctgaac cccgacagag agtacgactt     4620

cagagacctg acctggtgca tcaacccccc cgagagaatc aagctggact acgaccagta     4680

ctgcgccgac gtggccgccg aggagctgat gaacgccctg gtgaacagca ccctgctgga     4740

gaccagaacc accaaccagt tcctggccgt gagcaagggc aactgcagcg gccccaccac     4800

catcagaggc cagttcagca acatgagcct gagcctgctg gacctgtacc tgggcagagg     4860

ctacaacgtg agcagcatcg tgaccatgac cagccagggc atgtacggcg gcacctacct     4920

ggtggagaag cccaacctga gcagcaagag aagcgagctg agccagctga gcatgtacag     4980

agtgttcgag gtgggcgtga tcagaaaccc cggcctgggc gcccccgtgt tccacatgac     5040

caactacctg gagcagcccg tgagcaacga cctgagcaac tgcatggtgg ccctgggcga     5100

gctgaagctg gccgccctgt gccacggcga ggacagcatc accatcccct accagggcag     5160

cggcaagggc gtgagcttcc agctggtgaa gctgggcgtg tggaagagcc ccaccgacat     5220

gcagagctgg gtgcccctga gcaccgacga ccccgtgatc gacagactgt acctgagcag     5280

ccacagaggc gtgatcgccg acaaccaggc caagtgggcc gtgcccacca ccagaaccga     5340

cgacaagctg agaatggaga cctgcttcca gcaggcctgc aagggcaaga tccaggccct     5400

gtgcgagaac cccgagtggg cccccctgaa ggacaacaga atccccagct acggcgtgct     5460

gagcgtggac ctgagcctga ccgtggagct gaagatcaag atcgccagcg gcttcggccc     5520

cctgatcacc cacggcagcg gcatggacct gtacaagagc aaccacaaca acgtgtactg     5580

gctgaccatc ccccccatga agaacctggc cctgggcgtg atcaacaccc tggagtggat     5640

ccccagattc aaggtgagcc ccgccctgtt caccgtgccc atcaaggagg ccggcggcga     5700

ctgccacgcc cccacctacc tgcccgccga ggtggacggc gacgtgaagc tgagcagcaa     5760

cctggtgatc ctgcccggcc aggacctgca gtacgtgctg gccacctacg acaccagcgc     5820

cgtggagcac gccgtggtgt actacgtgta cagccccagc agactgagca gctacttcta     5880

ccccttcaga ctgcccatca agggcgtgcc catcgagctg caggtggagt gcttcacctg     5940

ggaccagaag ctgtggtgca gacacttctg cgtgctggcc gacagcgaga gcggcggcca     6000

catcacccac agcggcatgg tgggcatggg cgtgagctgc accgtgacca gagaggacgg     6060

caccaacggc ggcggcggca gcggcggcgg cggcagcggc ggcggcggca gcgccgccca     6120

gcccgccgag gtgcagctgc agcagagcgg cgccgagctg gtgaagcccg gcgccagcgt     6180

gaagctgagc tgcaccgcca gcggcttcaa catcaaggac acctacatcc acttcgtgag     6240

acagagaccc gagcagggcc tggagtggat cggcagaatc gaccccgcca acgacaacac     6300

cctgtacgcc agcaagttcc agggcaaggc caccatcacc gccgacacca gcagcaacac     6360

cgcctacatg cacctgtgca gcctgaccag cggcgacacc gccgtgtact actgcggcag     6420

aggctacggc tactacgtgt tcgaccactg gggccagggc accaccctga ccgtgagcag     6480

cggcggcggc ggcagcggag gaggaggatc tggggggggg gggtccgacg tgcagatcaa     6540

ccagagcccc agcttcctgg ccgccagccc cggcgagacc atcaccatca actgcagaac     6600

cagcagaagc atcagccagt acctggcctg gtaccaggag aagcccggca agaccaacaa     6660

gctgctgatc tacagcggca gcaccctgca gagcggcatc cccagcagat tcagcggcag     6720

cggcagcggc accgacttca ccctgaccat cagcggcctg gagcccgagg acttcgccat     6780

gtactactgc cagcagcaca acgagaaccc cctgaccttc ggcgccggca ccaagctgga     6840

gctgaag                                                               6847


<210>  22
<211>  4712
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Construct 6: pBA_9b-hCD19CAR-mir223

<400>  22
ctagcttaag taacgccatt ttgcaaggca tggaaaaata cataactgag aatagagaag       60

ttcagatcaa ggtcaggaac agatggaaca gctgaatatg ggccaaacag gatatctgtg      120

gtaagcagtt cctgccccgg ctcagggcca agaacagatg gaacagctga atatgggcca      180

aacaggatat ctgtggtaag cagttcctgc cccggctcag ggccaagaac agatggtccc      240

cagatgcggt ccagccctca gcagtttcta gagaaccatc agatgtttcc agggtgcccc      300

aaggacctga aatgaccctg tgccttattt gaactaacca atcagttcgc ttctcgcttc      360

tgttcgcgcg cttctgctcc ccgagctcaa taaaagagcc cacaacccct cactcggcgc      420

gccagtcctc cgattgactg agtcgcccgg gtacccgtgt atccaataaa ccctcttgca      480

gttgcatccg acttgtggtc tcgctgttcc ttgggagggt ctcctctgag tgattgacta      540

cccgtcagcg ggggtctttc atttgggggc tcgtccggga tcgggagacc cctgcccagg      600

gaccaccgac ccaccaccgg gaggtaagct ggccagcaac ttatctgtgt ctgtccgatt      660

gtctagtgtc tatgactgat tttatgcgcc tgcgtcggta ctagttagct aactagctct      720

gtatctggcg gacccgtggt ggaactgacg agttcggaac acccggccgc aaccctggga      780

gacgtcccag ggacttcggg ggccgttttt gtggcccgac ctgagtccaa aaatcccgat      840

cgttttggac tctttggtgc acccccctta gaggagggat atgtggttct ggtaggagac      900

gagaacctaa aacagttccc gcctccgtct gaatttttgc tttcggtttg ggaccgaagc      960

cgcgccgcgc gtcttgtctg ctgcagcatc gttctgtgtt gtctctgtct gactgtgttt     1020

ctgtatttgt ctgagaatta aggccagact gttaccactc cctgaagttt gaccttaggt     1080

cactggaaag atgtcgagcg gatcgctcac aaccagtcgg tagatgtcaa gaagagacgt     1140

tgggttacct tctgctctgc agaatggcca acctttaacg tcggatggcc gcgagacggc     1200

acctttaacc gagacctcat cacccaggtt aagatcaagg tcttttcacc tggcccgcat     1260

ggacacccag accaggtccc ctacatcgtg acctgggaag ccttggcttt tgacccccct     1320

ccctgggtca agccctttgt acaccctaag cctccgcctc ctcttcctcc atccgccccg     1380

tctctccccc ttgaacctcc tcgttcgacc ccgcctcgat cctcccttta tccagccctc     1440

actccttctc taggcgccgg aattaattct cgaggggccc agatctgcgg ccgcatggcc     1500

ttaccagtga ccgccttgct cctgccgctg gccttgctgc tccacgccgc caggccggac     1560

atccagatga cacagactac atcctccctg tctgcctctc tgggagacag agtcaccatc     1620

agttgcaggg caagtcagga cattagtaaa tatttaaatt ggtatcagca gaaaccagat     1680

ggaactgtta aactcctgat ctaccataca tcaagattac actcaggagt cccatcaagg     1740

ttcagtggca gtgggtctgg aacagattat tctctcacca ttagcaacct ggagcaagaa     1800

gatattgcca cttacttttg ccaacagggt aatacgcttc cgtacacgtt cggagggggg     1860

accaagctgg agatcacagg tggcggtggc tcgggcggtg gtgggtcggg tggcggcgga     1920

tctgaggtga aactgcagga gtcaggacct ggcctggtgg cgccctcaca gagcctgtcc     1980

gtcacatgca ctgtctcagg ggtctcatta cccgactatg gtgtaagctg gattcgccag     2040

cctccacgaa agggtctgga gtggctggga gtaatatggg gtagtgaaac cacatactat     2100

aattcagctc tcaaatccag actgaccatc atcaaggaca actccaagag ccaagttttc     2160

ttaaaaatga acagtctgca aactgatgac acagccattt actactgtgc caaacattat     2220

tactacggtg gtagctatgc tatggactac tggggccaag gaacctcagt caccgtctcc     2280

tcaaccacga cgccagcgcc gcgaccacca acaccggcgc ccaccatcgc gtcgcagccc     2340

ctgtccctgc gcccagaggc gtgccggcca gcggcggggg gcgcagtgca cacgaggggg     2400

ctggacttcg cctgtgatat ctacatctgg gcgcccttgg ccgggacttg tggggtcctt     2460

ctcctgtcac tggttatcac cctttactgc aaacggggca gaaagaaact cctgtatata     2520

ttcaaacaac catttatgag accagtacaa actactcaag aggaagatgg ctgtagctgc     2580

cgatttccag aagaagaaga aggaggatgt gaactgagag tgaagttcag caggagcgca     2640

gacgcccccg cgtacaagca gggccagaac cagctctata acgagctcaa tctaggacga     2700

agagaggagt acgatgtttt ggacaagaga cgtggccggg accctgagat ggggggaaag     2760

ccgagaagga agaaccctca ggaaggcctg tacaatgaac tgcagaaaga taagatggcg     2820

gaggcctaca gtgagattgg gatgaaaggc gagcgccgga ggggcaaggg gcacgatggc     2880

ctttaccagg gtctcagtac agccaccaag gacacctacg acgcccttca catgcaggcc     2940

ctgccccctc gctaggtcga cacgcgttac tggccgaagc cgcttggaat aaggccggtg     3000

tgcgtttgtc tatatgttat tttccaccat attgccgtct tttggcaatg tgagggcccg     3060

gaaacctggc cctgtcttct tgacgagcat tcctaggggt ctttcccctc tcgccaaagg     3120

aatgcaaggt ctgttgaatg tcgtgaagga agcagttcct ctggaagctt cttgaagaca     3180

aacaacgtct gtagcgaccc tttgcaggca gcggaacccc ccacctggcg acaggtgcct     3240

ctgcggccaa aagccacgtg tataagatac acctgcaaag gcggcacaac cccagtgcca     3300

cgttgtgagt tggatagttg tggaaagagt caaatggctc tcctcaagcg tattcaacaa     3360

ggggctgaag gatgcccaga aggtacccca ttgtatggga tctgatctgg ggcctcggtg     3420

cacatgcttt acatgtgttt agtcgaggtt aaaaaacgtc taggcccccc gaaccacggg     3480

gacgtggttt tcctttgaaa aacacgatta taaatggtga ccggcggcat ggcctccaag     3540

tgggatcaaa agggcatgga tatcgcttac gaggaggccc tgctgggcta caaggagggc     3600

ggcgtgccta tcggcggctg tctgatcaac aacaaggacg gcagtgtgct gggcaggggc     3660

cacaacatga ggttccagaa gggctccgcc accctgcacg gcgagatctc caccctggag     3720

aactgtggca ggctggaggg caaggtgtac aaggacacca ccctgtacac caccctgtcc     3780

ccttgtgaca tgtgtaccgg cgctatcatc atgtacggca tccctaggtg tgtgatcggc     3840

gagaacgtga acttcaagtc caagggcgag aagtacctgc aaaccagggg ccacgaggtg     3900

gtggttgttg acgatgagag gtgtaagaag ctgatgaagc agttcatcga cgagaggcct     3960

caggactggt tcgaggatat cggcgagtaa tcgttggggt atttgacaaa ctgacacgta     4020

tggggtattt gacaaactga catcgatggg gtatttgaca aactgacatc actggggtat     4080

ttgacaaact gacagtcgac tctccagaaa aaggggggaa tgaaagaccc cacctgtagg     4140

tttggcaagc tagcttaagt aacgccattt tgcaaggcat ggaaaaatac ataactgaga     4200

atagagaagt tcagatcaag gtcaggaaca gatggaacag ctgaatatgg gccaaacagg     4260

atatctgtgg taagcagttc ctgccccggc tcagggccaa gaacagatgg aacagctgaa     4320

tatgggccaa acaggatatc tgtggtaagc agttcctgcc ccggctcagg gccaagaaca     4380

gatggtcccc agatgcggtc cagccctcag cagtttctag agaaccatca gatgtttcca     4440

gggtgcccca aggacctgaa atgaccctgt gccttatttg aactaaccaa tcagttcgct     4500

tctcgcttct gttcgcgcgc ttctgctccc cgagctcaat aaaagagccc acaacccctc     4560

actcggcgcg ccagtcctcc gattgactga gtcgcccggg tacccgtgta tccaataaac     4620

cctcttgcag ttgcatccga cttgtggtct cgctgttcct tgggagggtc tcctctgagt     4680

gattgactac ccgtcagcgg gggtctttca tt                                   4712


<210>  23
<211>  7294
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Construct 56: pBA-9b-BCMACAR-IRES-CD

<400>  23
tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca       60

cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg      120

ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc      180

accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc      240

attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat      300

tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt      360

tttcccagtc acgacgttgt aaaacgacgg ccagtgccaa gctgactcta gaggatcgat      420

ccccggccgc tctagcttaa gtaacgccat tttgcaaggc atggaaaaat acataactga      480

gaatagagaa gttcagatca aggtcaggaa cagatggaac agctgaatat gggccaaaca      540

ggatatctgt ggtaagcagt tcctgccccg gctcagggcc aagaacagat ggaacagctg      600

aatatgggcc aaacaggata tctgtggtaa gcagttcctg ccccggctca gggccaagaa      660

cagatggtcc ccagatgcgg tccagccctc agcagtttct agagaaccat cagatgtttc      720

cagggtgccc caaggacctg aaatgaccct gtgccttatt tgaactaacc aatcagttcg      780

cttctcgctt ctgttcgcgc gcttctgctc cccgagctca ataaaagagc ccacaacccc      840

tcactcggcg cgccagtcct ccgattgact gagtcgcccg ggtacccgtg tatccaataa      900

accctcttgc agttgcatcc gacttgtggt ctcgctgttc cttgggaggg tctcctctga      960

gtgattgact acccgtcagc gggggtcttt catttggggg ctcgtccggg atcgggagac     1020

ccctgcccag ggaccaccga cccaccaccg ggaggtaagc tggccagcaa cttatctgtg     1080

tctgtccgat tgtctagtgt ctatgactga ttttatgcgc ctgcgtcggt actagttagc     1140

taactagctc tgtatctggc ggacccgtgg tggaactgac gagttcggaa cacccggccg     1200

caaccctggg agacgtccca gggacttcgg gggccgtttt tgtggcccga cctgagtcca     1260

aaaatcccga tcgttttgga ctctttggtg cacccccctt agaggaggga tatgtggttc     1320

tggtaggaga cgagaaccta aaacagttcc cgcctccgtc tgaatttttg ctttcggttt     1380

gggaccgaag ccgcgccgcg cgtcttgtct gctgcagcat cgttctgtgt tgtctctgtc     1440

tgactgtgtt tctgtatttg tctgagaatt aaggccagac tgttaccact ccctgaagtt     1500

tgaccttagg tcactggaaa gatgtcgagc ggatcgctca caaccagtcg gtagatgtca     1560

agaagagacg ttgggttacc ttctgctctg cagaatggcc aacctttaac gtcggatggc     1620

cgcgagacgg cacctttaac cgagacctca tcacccaggt taagatcaag gtcttttcac     1680

ctggcccgca tggacaccca gaccaggtcc cctacatcgt gacctgggaa gccttggctt     1740

ttgacccccc tccctgggtc aagccctttg tacaccctaa gcctccgcct cctcttcctc     1800

catccgcccc gtctctcccc cttgaacctc ctcgttcgac cccgcctcga tcctcccttt     1860

atccagccct cactccttct ctaggcgccg gaattaattc tcgaggggcc cagatctgcg     1920

gccgcatggc cctgcccgtg accgccctgc tgctgcccct ggccctgctg ctgcacgccg     1980

ccagacccca ggtgcagctg gtgcagagcg gcgccgaggt gaagaagccc ggcgccagcg     2040

tgaaggtgag ctgcaaggcc agcggctaca gcttccccga ctactacatc aactgggtga     2100

gacaggcccc cggccagggc ctggagtgga tgggctggat ctacttcgcc agcggcaaca     2160

gcgagtacaa ccagaagttc accggcagag tgaccatgac cagagacacc agcagcagca     2220

ccgcctacat ggagctgagc agcctgagaa gcgaggacac cgccgtgtac ttctgcgcca     2280

gcctgtacga ctacgactgg tacttcgacg tgtggggcca gggcaccatg gtgaccgtga     2340

gcagcggcgg cggcggcagc ggcggcggcg gcagcggcgg cggcggcagc gacatcgtga     2400

tgacccagac ccccctgagc ctgagcgtga cccccggcga gcccgccagc atcagctgct     2460

ggagcagcca gagcctggtg cacagcaacg gcaacaccta cctgcactgg tacctgcaga     2520

agcccggcca gagcccccag ctgctgatct acaaggtgag caacagattc agcggcgtgc     2580

ccgacagatt cagcggcagc ggcagcggcg ccgacttcac cctgaagatc agcagagtgg     2640

aggccgagga cgtgggcgtg tactactgcg ccgagaccag ccacgtgccc tggaccttcg     2700

gccagggcac caagctggag atcaagacca ccacccccgc ccccagaccc cccacccccg     2760

cccccaccat cgccagccag cccctgagcc tgagacccga ggcctgcaga cccgccgccg     2820

gcggcgccgt gcacaccaga ggcctggact tcgcctgcga catctacatc tgggcccccc     2880

tggccggcac ctgcggcgtg ctgctgctga gcctggtgat caccctgtac tgcaagagag     2940

gcagaaagaa gctgctgtac atcttcaagc agcccttcat gagacccgtg cagaccaccc     3000

aggaggagga cggctgcagc tgcagattcc ccgaggagga ggagggcggc tgcgagctga     3060

gagtgaagtt cagcagaagc gccgacgccc ccgcctacca gcagggccag aaccagctgt     3120

acaacgagct gaacctgggc agaagagagg agtacgacgt gctggacaag agaagaggca     3180

gagaccccga gatgggcggc aagcccagaa gaaagaaccc ccaggagggc ctgtacaacg     3240

agctgcagaa ggacaagatg gccgaggcct acagcgagat cggcatgaag ggcgagagaa     3300

gaagaggcaa gggccacgac ggcctgtacc agggcctgag caccgccacc aaggacacct     3360

acgacgccct gcacatgcag gccctgcccc ccagataagt cgacacgcgt tactggccga     3420

agccgcttgg aataaggccg gtgtgcgttt gtctatatgt tattttccac catattgccg     3480

tcttttggca atgtgagggc ccggaaacct ggccctgtct tcttgacgag cattcctagg     3540

ggtctttccc ctctcgccaa aggaatgcaa ggtctgttga atgtcgtgaa ggaagcagtt     3600

cctctggaag cttcttgaag acaaacaacg tctgtagcga ccctttgcag gcagcggaac     3660

cccccacctg gcgacaggtg cctctgcggc caaaagccac gtgtataaga tacacctgca     3720

aaggcggcac aaccccagtg ccacgttgtg agttggatag ttgtggaaag agtcaaatgg     3780

ctctcctcaa gcgtattcaa caaggggctg aaggatgccc agaaggtacc ccattgtatg     3840

ggatctgatc tggggcctcg gtgcacatgc tttacatgtg tttagtcgag gttaaaaaac     3900

gtctaggccc cccgaaccac ggggacgtgg ttttcctttg aaaaacacga ttataaatgg     3960

tgaccggcgg catggcctcc aagtgggatc aaaagggcat ggatatcgct tacgaggagg     4020

ccctgctggg ctacaaggag ggcggcgtgc ctatcggcgg ctgtctgatc aacaacaagg     4080

acggcagtgt gctgggcagg ggccacaaca tgaggttcca gaagggctcc gccaccctgc     4140

acggcgagat ctccaccctg gagaactgtg gcaggctgga gggcaaggtg tacaaggaca     4200

ccaccctgta caccaccctg tccccttgtg acatgtgtac cggcgctatc atcatgtacg     4260

gcatccctag gtgtgtgatc ggcgagaacg tgaacttcaa gtccaagggc gagaagtacc     4320

tgcaaaccag gggccacgag gtggtggttg ttgacgatga gaggtgtaag aagctgatga     4380

agcagttcat cgacgagagg cctcaggact ggttcgagga tatcggcgag taatgcgctc     4440

tccagaaaaa ggggggaatg aaagacccca cctgtaggtt tggcaagcta gcttaagtaa     4500

cgccattttg caaggcatgg aaaaatacat aactgagaat agagaagttc agatcaaggt     4560

caggaacaga tggaacagct gaatatgggc caaacaggat atctgtggta agcagttcct     4620

gccccggctc agggccaaga acagatggaa cagctgaata tgggccaaac aggatatctg     4680

tggtaagcag ttcctgcccc ggctcagggc caagaacaga tggtccccag atgcggtcca     4740

gccctcagca gtttctagag aaccatcaga tgtttccagg gtgccccaag gacctgaaat     4800

gaccctgtgc cttatttgaa ctaaccaatc agttcgcttc tcgcttctgt tcgcgcgctt     4860

ctgctccccg agctcaataa aagagcccac aacccctcac tcggcgcgcc agtcctccga     4920

ttgactgagt cgcccgggta cccgtgtatc caataaaccc tcttgcagtt gcatccgact     4980

tgtggtctcg ctgttccttg ggagggtctc ctctgagtga ttgactaccc gtcagcgggg     5040

gtctttcatt tgaagccgaa ttcgtaatca tggtcatagc tgtttcctgt gtgaaattgt     5100

tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt     5160

gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg     5220

ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg     5280

cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg     5340

cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat     5400

aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc     5460

gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc     5520

tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga     5580

agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt     5640

ctcccttcgg gaagcgtggc gctttctcaa agctcacgct gtaggtatct cagttcggtg     5700

taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc     5760

gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg     5820

gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc     5880

ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg     5940

ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc     6000

gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct     6060

caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt     6120

taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa     6180

aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa     6240

tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc     6300

tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct     6360

gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca     6420

gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt     6480

aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt     6540

gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc     6600

ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc     6660

tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt     6720

atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact     6780

ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc     6840

ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt     6900

ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg     6960

atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct     7020

gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa     7080

tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt     7140

ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc     7200

acatttcccc gaaaagtgcc acctgacgtc taagaaacca ttattatcat gacattaacc     7260

tataaaaata ggcgtatcac gaggcccttt cgtc                                 7294


<210>  24
<211>  9398
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Construct 57: pBA-9b-BCMACAR-IRES-CD-CMV-hIL12

<400>  24
tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca       60

cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg      120

ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc      180

accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc      240

attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat      300

tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt      360

tttcccagtc acgacgttgt aaaacgacgg ccagtgccaa gctgactcta gaggatcgat      420

ccccggccgc tctagcttaa gtaacgccat tttgcaaggc atggaaaaat acataactga      480

gaatagagaa gttcagatca aggtcaggaa cagatggaac agctgaatat gggccaaaca      540

ggatatctgt ggtaagcagt tcctgccccg gctcagggcc aagaacagat ggaacagctg      600

aatatgggcc aaacaggata tctgtggtaa gcagttcctg ccccggctca gggccaagaa      660

cagatggtcc ccagatgcgg tccagccctc agcagtttct agagaaccat cagatgtttc      720

cagggtgccc caaggacctg aaatgaccct gtgccttatt tgaactaacc aatcagttcg      780

cttctcgctt ctgttcgcgc gcttctgctc cccgagctca ataaaagagc ccacaacccc      840

tcactcggcg cgccagtcct ccgattgact gagtcgcccg ggtacccgtg tatccaataa      900

accctcttgc agttgcatcc gacttgtggt ctcgctgttc cttgggaggg tctcctctga      960

gtgattgact acccgtcagc gggggtcttt catttggggg ctcgtccggg atcgggagac     1020

ccctgcccag ggaccaccga cccaccaccg ggaggtaagc tggccagcaa cttatctgtg     1080

tctgtccgat tgtctagtgt ctatgactga ttttatgcgc ctgcgtcggt actagttagc     1140

taactagctc tgtatctggc ggacccgtgg tggaactgac gagttcggaa cacccggccg     1200

caaccctggg agacgtccca gggacttcgg gggccgtttt tgtggcccga cctgagtcca     1260

aaaatcccga tcgttttgga ctctttggtg cacccccctt agaggaggga tatgtggttc     1320

tggtaggaga cgagaaccta aaacagttcc cgcctccgtc tgaatttttg ctttcggttt     1380

gggaccgaag ccgcgccgcg cgtcttgtct gctgcagcat cgttctgtgt tgtctctgtc     1440

tgactgtgtt tctgtatttg tctgagaatt aaggccagac tgttaccact ccctgaagtt     1500

tgaccttagg tcactggaaa gatgtcgagc ggatcgctca caaccagtcg gtagatgtca     1560

agaagagacg ttgggttacc ttctgctctg cagaatggcc aacctttaac gtcggatggc     1620

cgcgagacgg cacctttaac cgagacctca tcacccaggt taagatcaag gtcttttcac     1680

ctggcccgca tggacaccca gaccaggtcc cctacatcgt gacctgggaa gccttggctt     1740

ttgacccccc tccctgggtc aagccctttg tacaccctaa gcctccgcct cctcttcctc     1800

catccgcccc gtctctcccc cttgaacctc ctcgttcgac cccgcctcga tcctcccttt     1860

atccagccct cactccttct ctaggcgccg gaattaattc tcgaggggcc cagatctgcg     1920

gccgcatggc cctgcccgtg accgccctgc tgctgcccct ggccctgctg ctgcacgccg     1980

ccagacccca ggtgcagctg gtgcagagcg gcgccgaggt gaagaagccc ggcgccagcg     2040

tgaaggtgag ctgcaaggcc agcggctaca gcttccccga ctactacatc aactgggtga     2100

gacaggcccc cggccagggc ctggagtgga tgggctggat ctacttcgcc agcggcaaca     2160

gcgagtacaa ccagaagttc accggcagag tgaccatgac cagagacacc agcagcagca     2220

ccgcctacat ggagctgagc agcctgagaa gcgaggacac cgccgtgtac ttctgcgcca     2280

gcctgtacga ctacgactgg tacttcgacg tgtggggcca gggcaccatg gtgaccgtga     2340

gcagcggcgg cggcggcagc ggcggcggcg gcagcggcgg cggcggcagc gacatcgtga     2400

tgacccagac ccccctgagc ctgagcgtga cccccggcga gcccgccagc atcagctgct     2460

ggagcagcca gagcctggtg cacagcaacg gcaacaccta cctgcactgg tacctgcaga     2520

agcccggcca gagcccccag ctgctgatct acaaggtgag caacagattc agcggcgtgc     2580

ccgacagatt cagcggcagc ggcagcggcg ccgacttcac cctgaagatc agcagagtgg     2640

aggccgagga cgtgggcgtg tactactgcg ccgagaccag ccacgtgccc tggaccttcg     2700

gccagggcac caagctggag atcaagacca ccacccccgc ccccagaccc cccacccccg     2760

cccccaccat cgccagccag cccctgagcc tgagacccga ggcctgcaga cccgccgccg     2820

gcggcgccgt gcacaccaga ggcctggact tcgcctgcga catctacatc tgggcccccc     2880

tggccggcac ctgcggcgtg ctgctgctga gcctggtgat caccctgtac tgcaagagag     2940

gcagaaagaa gctgctgtac atcttcaagc agcccttcat gagacccgtg cagaccaccc     3000

aggaggagga cggctgcagc tgcagattcc ccgaggagga ggagggcggc tgcgagctga     3060

gagtgaagtt cagcagaagc gccgacgccc ccgcctacca gcagggccag aaccagctgt     3120

acaacgagct gaacctgggc agaagagagg agtacgacgt gctggacaag agaagaggca     3180

gagaccccga gatgggcggc aagcccagaa gaaagaaccc ccaggagggc ctgtacaacg     3240

agctgcagaa ggacaagatg gccgaggcct acagcgagat cggcatgaag ggcgagagaa     3300

gaagaggcaa gggccacgac ggcctgtacc agggcctgag caccgccacc aaggacacct     3360

acgacgccct gcacatgcag gccctgcccc ccagataagt cgacacgcgt tactggccga     3420

agccgcttgg aataaggccg gtgtgcgttt gtctatatgt tattttccac catattgccg     3480

tcttttggca atgtgagggc ccggaaacct ggccctgtct tcttgacgag cattcctagg     3540

ggtctttccc ctctcgccaa aggaatgcaa ggtctgttga atgtcgtgaa ggaagcagtt     3600

cctctggaag cttcttgaag acaaacaacg tctgtagcga ccctttgcag gcagcggaac     3660

cccccacctg gcgacaggtg cctctgcggc caaaagccac gtgtataaga tacacctgca     3720

aaggcggcac aaccccagtg ccacgttgtg agttggatag ttgtggaaag agtcaaatgg     3780

ctctcctcaa gcgtattcaa caaggggctg aaggatgccc agaaggtacc ccattgtatg     3840

ggatctgatc tggggcctcg gtgcacatgc tttacatgtg tttagtcgag gttaaaaaac     3900

gtctaggccc cccgaaccac ggggacgtgg ttttcctttg aaaaacacga ttataaatgg     3960

tgaccggcgg catggcctcc aagtgggatc aaaagggcat ggatatcgct tacgaggagg     4020

ccctgctggg ctacaaggag ggcggcgtgc ctatcggcgg ctgtctgatc aacaacaagg     4080

acggcagtgt gctgggcagg ggccacaaca tgaggttcca gaagggctcc gccaccctgc     4140

acggcgagat ctccaccctg gagaactgtg gcaggctgga gggcaaggtg tacaaggaca     4200

ccaccctgta caccaccctg tccccttgtg acatgtgtac cggcgctatc atcatgtacg     4260

gcatccctag gtgtgtgatc ggcgagaacg tgaacttcaa gtccaagggc gagaagtacc     4320

tgcaaaccag gggccacgag gtggtggttg ttgacgatga gaggtgtaag aagctgatga     4380

agcagttcat cgacgagagg cctcaggact ggttcgagga tatcggcgag taaattagtc     4440

gactgcgctg acattgatta ttgactagtt attaatagta atcaattacg gggtcattag     4500

ttcatagccc atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct     4560

gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc     4620

caatagggac tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg     4680

cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat     4740

ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca     4800

tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc     4860

gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga     4920

gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat     4980

tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta tataagcaga gcttaaggcc     5040

accatgtggc cccccggcag cgccagccag ccccccccca gccccgccgc cgccaccggc     5100

ctgcaccccg ccgccagacc cgtgagcctg cagtgcagac tgagcatgtg ccccgccaga     5160

agcctgctgc tggtggccac cctggtgctg ctggaccacc tgagcctggc cagaaacctg     5220

cccgtggcca cccccgaccc cggcatgttc ccctgcctgc accacagcca gaacctgctg     5280

agagccgtga gcaacatgct gcagaaggcc agacagaccc tggagttcta cccctgcacc     5340

agcgaggaga tcgaccacga ggacatcacc aaggacaaga ccagcaccgt ggaggcctgc     5400

ctgcccctgg agctgaccaa gaacgagagc tgcctgaaca gcagagagac cagcttcatc     5460

accaacggca gctgcctggc cagcagaaag accagcttca tgatggccct gtgcctgagc     5520

agcatctacg aggacctgaa gatgtaccag gtggagttca agaccatgaa cgccaagctg     5580

ctgatggacc ccaagagaca gatcttcctg gaccagaaca tgctggccgt gatcgacgag     5640

ctgatgcagg ccctgaactt caacagcgag accgtgcccc agaagagcag cctggaggag     5700

cccgacttct acaagaccaa gatcaagctg tgcatcctgc tgcacgcctt cagaatcaga     5760

gccgtgacca tcgacagagt gatgagctac ctgaacgcca gcggcagcac cagcggcagc     5820

ggcctgcccg gcagcggcgg cggcagcacc ctgggcagaa acctgcccgt ggccaccccc     5880

gaccccggca tgttcccctg cctgcaccac agccagaacc tgctgagagc cgtgagcaac     5940

atgctgcaga aggccagaca gaccctggag ttctacccct gcaccagcga ggagatcgac     6000

cacgaggaca tcaccaagga caagaccagc accgtggagg cctgcctgcc cctggagctg     6060

accaagaacg agagctgcct gaacagcaga gagaccagct tcatcaccaa cggcagctgc     6120

ctggccagca gaaagaccag cttcatgatg gccctgtgcc tgagcagcat ctacgaggac     6180

ctgaagatgt accaggtgga gttcaagacc atgaacgcca agctgctgat ggaccccaag     6240

agacagatct tcctggacca gaacatgctg gccgtgatcg acgagctgat gcaggccctg     6300

aacttcaaca gcgagaccgt gccccagaag agcagcctgg aggagcccga cttctacaag     6360

accaagatca agctgtgcat cctgctgcac gccttcagaa tcagagccgt gaccatcgac     6420

agagtgatga gctacctgaa cgccagcgat caagcttgga tccatcgata aaataaaaga     6480

ttttatttag tctccagaaa aaggggggaa tgaaagaccc cacctgtagg tttggcaagc     6540

tatctccaga aaaagggggg aatgaaagac cccacctgta ggtttggcaa gctagcttaa     6600

gtaacgccat tttgcaaggc atggaaaaat acataactga gaatagagaa gttcagatca     6660

aggtcaggaa cagatggaac agctgaatat gggccaaaca ggatatctgt ggtaagcagt     6720

tcctgccccg gctcagggcc aagaacagat ggaacagctg aatatgggcc aaacaggata     6780

tctgtggtaa gcagttcctg ccccggctca gggccaagaa cagatggtcc ccagatgcgg     6840

tccagccctc agcagtttct agagaaccat cagatgtttc cagggtgccc caaggacctg     6900

aaatgaccct gtgccttatt tgaactaacc aatcagttcg cttctcgctt ctgttcgcgc     6960

gcttctgctc cccgagctca ataaaagagc ccacaacccc tcactcggcg cgccagtcct     7020

ccgattgact gagtcgcccg ggtacccgtg tatccaataa accctcttgc agttgcatcc     7080

gacttgtggt ctcgctgttc cttgggaggg tctcctctga gtgattgact acccgtcagc     7140

gggggtcttt catttgaagc cgaattcgta atcatggtca tagctgtttc ctgtgtgaaa     7200

ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg     7260

gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca     7320

gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg     7380

tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg     7440

gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg     7500

ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa     7560

ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg     7620

acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc     7680

tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc     7740

ctttctccct tcgggaagcg tggcgctttc tcaaagctca cgctgtaggt atctcagttc     7800

ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg     7860

ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc     7920

actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga     7980

gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc     8040

tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac     8100

caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg     8160

atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc     8220

acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa     8280

ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta     8340

ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt     8400

tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag     8460

tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca     8520

gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc     8580

tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt     8640

tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag     8700

ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt     8760

tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat     8820

ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt     8880

gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc     8940

ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat     9000

cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag     9060

ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt     9120

ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg     9180

gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta     9240

ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc     9300

gcgcacattt ccccgaaaag tgccacctga cgtctaagaa accattatta tcatgacatt     9360

aacctataaa aataggcgta tcacgaggcc ctttcgtc                             9398


<210>  25
<211>  6051
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  vBA-9b-hCD19CAR-IRES-TKmiRT+CMVprom-IL15

<400>  25
gcgccagucc uccgauugac ugagucgccc ggguacccgu guauccaaua aacccucuug       60

caguugcauc cgacuugugg ucucgcuguu ccuugggagg gucuccucug agugauugac      120

uacccgucag cgggggucuu ucauuugggg gcucguccgg gaucgggaga ccccugccca      180

gggaccaccg acccaccacc gggagguaag cuggccagca acuuaucugu gucuguccga      240

uugucuagug ucuaugacug auuuuaugcg ccugcgucgg uacuaguuag cuaacuagcu      300

cuguaucugg cggacccgug guggaacuga cgaguucgga acacccggcc gcaacccugg      360

gagacguccc agggacuucg ggggccguuu uuguggcccg accugagucc aaaaaucccg      420

aucguuuugg acucuuuggu gcaccccccu uagaggaggg auaugugguu cugguaggag      480

acgagaaccu aaaacaguuc ccgccuccgu cugaauuuuu gcuuucgguu ugggaccgaa      540

gccgcgccgc gcgucuuguc ugcugcagca ucguucugug uugucucugu cugacugugu      600

uucuguauuu gucugagaau uaaggccaga cuguuaccac ucccugaagu uugaccuuag      660

gucacuggaa agaugucgag cggaucgcuc acaaccaguc gguagauguc aagaagagac      720

guuggguuac cuucugcucu gcagaauggc caaccuuuaa cgucggaugg ccgcgagacg      780

gcaccuuuaa ccgagaccuc aucacccagg uuaagaucaa ggucuuuuca ccuggcccgc      840

auggacaccc agaccagguc cccuacaucg ugaccuggga agccuuggcu uuugaccccc      900

cucccugggu caagcccuuu guacacccua agccuccgcc uccucuuccu ccauccgccc      960

cgucucuccc ccuugaaccu ccucguucga ccccgccucg auccucccuu uauccagccc     1020

ucacuccuuc ucuaggcgcc ggaauuaauu cucgaggggc ccagaucugc ggccgcaugg     1080

ccuuaccagu gaccgccuug cuccugccgc uggccuugcu gcuccacgcc gccaggccgg     1140

acauccagau gacacagacu acauccuccc ugucugccuc ucugggagac agagucacca     1200

ucaguugcag ggcaagucag gacauuagua aauauuuaaa uugguaucag cagaaaccag     1260

auggaacugu uaaacuccug aucuaccaua caucaagauu acacucagga gucccaucaa     1320

gguucagugg cagugggucu ggaacagauu auucucucac cauuagcaac cuggagcaag     1380

aagauauugc cacuuacuuu ugccaacagg guaauacgcu uccguacacg uucggagggg     1440

ggaccaagcu ggagaucaca gguggcggug gcucgggcgg uggugggucg gguggcggcg     1500

gaucugaggu gaaacugcag gagucaggac cuggccuggu ggcgcccuca cagagccugu     1560

ccgucacaug cacugucuca ggggucucau uacccgacua ugguguaagc uggauucgcc     1620

agccuccacg aaagggucug gaguggcugg gaguaauaug ggguagugaa accacauacu     1680

auaauucagc ucucaaaucc agacugacca ucaucaagga caacuccaag agccaaguuu     1740

ucuuaaaaau gaacagucug caaacugaug acacagccau uuacuacugu gccaaacauu     1800

auuacuacgg ugguagcuau gcuauggacu acuggggcca aggaaccuca gucaccgucu     1860

ccucaaccac gacgccagcg ccgcgaccac caacaccggc gcccaccauc gcgucgcagc     1920

cccugucccu gcgcccagag gcgugccggc cagcggcggg gggcgcagug cacacgaggg     1980

ggcuggacuu cgccugugau aucuacaucu gggcgcccuu ggccgggacu uguggggucc     2040

uucuccuguc acugguuauc acccuuuacu gcaaacgggg cagaaagaaa cuccuguaua     2100

uauucaaaca accauuuaug agaccaguac aaacuacuca agaggaagau ggcuguagcu     2160

gccgauuucc agaagaagaa gaaggaggau gugaacugag agugaaguuc agcaggagcg     2220

cagacgcccc cgcguacaag cagggccaga accagcucua uaacgagcuc aaucuaggac     2280

gaagagagga guacgauguu uuggacaaga gacguggccg ggacccugag auggggggaa     2340

agccgagaag gaagaacccu caggaaggcc uguacaauga acugcagaaa gauaagaugg     2400

cggaggccua cagugagauu gggaugaaag gcgagcgccg gaggggcaag gggcacgaug     2460

gccuuuacca gggucucagu acagccacca aggacaccua cgacgcccuu cacaugcagg     2520

cccugccccc ucgcuagguc gacacgcguu acuggccgaa gccgcuugga auaaggccgg     2580

ugugcguuug ucuauauguu auuuuccacc auauugccgu cuuuuggcaa ugugagggcc     2640

cggaaaccug gcccugucuu cuugacgagc auuccuaggg gucuuucccc ucucgccaaa     2700

ggaaugcaag gucuguugaa ugucgugaag gaagcaguuc cucuggaagc uucuugaaga     2760

caaacaacgu cuguagcgac ccuuugcagg cagcggaacc ccccaccugg cgacaggugc     2820

cucugcggcc aaaagccacg uguauaagau acaccugcaa aggcggcaca accccagugc     2880

cacguuguga guuggauagu uguggaaaga gucaaauggc ucuccucaag cguauucaac     2940

aaggggcuga aggaugccca gaagguaccc cauuguaugg gaucugaucu ggggccucgg     3000

ugcacaugcu uuacaugugu uuagucgagg uuaaaaaacg ucuaggcccc ccgaaccacg     3060

gggacguggu uuuccuuuga aaaacacgau uauaaauggc uucauauccu ugccaccaac     3120

augcuuccgc uuucgaccaa gccgcacggu cuaggggcca caauaaucgc cggacugccc     3180

ugcggccucg gagacagcag aaggcaaccg aagucaggcu cgagcaaaag augccaaccc     3240

uccugcgggu cuauaucgau ggaccccaug gaauggggaa gaccacuacc acacaacucc     3300

ugguggcacu cgguagccgg gacgacaucg ucuacgugcc cgaacccaug acuuacuggc     3360

ggguucucgg ugcuuccgag acaaucgcca auaucuacac aacccaacac cgccucgauc     3420

aaggagaaau uagcgcaggg gacgcugccg uggugaugac aucagcccaa aucaccaugg     3480

gaaugcccua cgccgucacc gaugcugucc uggcaccaca cauuggcgga gaggccgggu     3540

caagucaugc accaccacca gcccugacua ucuuucucga ccggcaucca auugcauuca     3600

ugcugugcua uccugccgca cgcuaccuga ugggaaguau gacaccacag gccguccucg     3660

ccuucguugc ucugaucccu ccaacccugc cuggcacuaa caucguucuc ggcgcacucc     3720

ccgaagacag acacauugau cggcuggcca agaggcaacg gccuggcgag agacucgauc     3780

uggcuaugcu ggcugcuauu aggagagugu acgggcugcu ggccaauacu gugagauacc     3840

uccaaggggg aggaagcugg cgcgaggauu ggggccaacu gucuggcgcu gcugugccac     3900

cucaaggcgc cgagccacag ucaaaugcug guccuaggcc ccacaucggc gauacucucu     3960

uuacacuguu ccgggcacca gagcugcucg caccuaaugg agaucuguac aauguuuucg     4020

cuugggcccu cgauguccug gcuaagcggc uccggccuau gcacguguuc auccucgacu     4080

acgaccagag cccagcuggu ugucgggaug cucuccugca acugaccagc gggauggugc     4140

agacacacgu uacuacuccc ggcuccaucc ccacuaucug ugaccucgcc cggacauuug     4200

cccgggaaau gggcgaagcc aacugaucgu gcggucccgc ggcgccccgc cucgaugcgg     4260

ucccgcggcg ccccgccugc augcgguccc gcggcgcccc gccucuacgc ggucccgcgg     4320

cgccccgccu auuauggggu auuugacaaa cugacacgua ugggguauuu gacaaacuga     4380

caucgauggg guauuugaca aacugacauc acugggguau uugacaaacu gacagucgac     4440

cuaugcacug ucuuagacau ugauuauuga cuaguuauua auaguaauca auuacggggu     4500

cauuaguuca uagcccauau auggaguucc gcguuacaua acuuacggua aauggcccgc     4560

cuggcugacc gcccaacgac ccccgcccau ugacgucaau aaugacguau guucccauag     4620

uaacgccaau agggacuuuc cauugacguc aaugggugga guauuuacgg uaaacugccc     4680

acuuggcagu acaucaagug uaucauaugc caaguacgcc cccuauugac gucaaugacg     4740

guaaauggcc cgccuggcau uaugcccagu acaugaccuu augggacuuu ccuacuuggc     4800

aguacaucua cguauuaguc aucgcuauua ccauggugau gcgguuuugg caguacauca     4860

augggcgugg auagcgguuu gacucacggg gauuuccaag ucuccacccc auugacguca     4920

augggaguuu guuuuggcac caaaaucaac gggacuuucc aaaaugucgu aacaacuccg     4980

ccccauugac gcaaaugggc gguaggcgug uacgguggga ggucuauaua agcagagcug     5040

ccaccauggc ccccagaaga gccagaggcu gcagaacccu gggccugccc gcccugcugc     5100

ugcugcugcu gcugagaccc cccgccacca gaggcaacug ggugaacgug aucagcgacc     5160

ugaagaagau cgaggaccug auccagagca ugcacaucga cgccacccug uacaccgaga     5220

gcgacgugca ccccagcugc aaggugaccg ccaugaagug cuuccugcug gagcugcagg     5280

ugaucagccu ggagagcggc gacgccagca uccacgacac cguggaggac cugaucaucc     5340

uggccaacaa cagccugagc agcaacggca acgugaccga gagcggcugc aaggagugcg     5400

aggagcugga ggagaagaac aucaaggagu uccugcagag cuucgugcac aucgugcaga     5460

uguucaucaa caccagcaag cuuggaucca ucgauaaaau aaaagauuuu auuuagucuc     5520

cagaaaaagg ggggaaugaa agaccccacc uguagguuug gcaagcuagc uuaaguaacg     5580

ccauuuugca aggcauggaa aaauacauaa cugagaauag agaaguucag aucaagguca     5640

ggaacagaug gaacagcuga auaugggcca aacaggauau cugugguaag caguuccugc     5700

cccggcucag ggccaagaac agauggaaca gcugaauaug ggccaaacag gauaucugug     5760

guaagcaguu ccugccccgg cucagggcca agaacagaug guccccagau gcgguccagc     5820

ccucagcagu uucuagagaa ccaucagaug uuuccagggu gccccaagga ccugaaauga     5880

cccugugccu uauuugaacu aaccaaucag uucgcuucuc gcuucuguuc gcgcgcuucu     5940

gcuccccgag cucaauaaaa gagcccacaa ccccucacuc ggcgcgccag uccuccgauu     6000

gacugagucg cccggguacc cguguaucca auaaacccuc uugcaguugc a              6051


<210>  26
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  MLV specific forward primer

<400>  26
gcgcctgcgt cggtactag                                                    19


<210>  27
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  MLV specific reverse primer

<400>  27
gactcaggtc gggccacaa                                                    19


<210>  28
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  MLV Probe sequence

<400>  28
agttcggaac acccggccgc                                                   20


