                         SEQUENCE LISTING

<110>  Illumina Inc.
       Illumina Singapore Pte. Ltd.
       National University of Singapore
       Nirantar, Saurabh Rajendra
       Chua, Jasmine  Puay Suan
       Peisajovich, Sergio
       Yew, Wen Shan
       Go, Maybelle Darlene Kho
 
<120>  THERMOSTABLE TERMINAL DEOXYNUCLEOTIDYL TRANSFERASE

<130>  47CX-311971-WO

<150>  63/023,734
<151>  2020-05-12

<160>  33    

<170>  PatentIn version 3.5

<210>  1
<211>  382
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Bos taurus TdT 139-520


<220>
<221>  NON_TER
<222>  (1)..(1)

<400>  1

Arg Thr Asp Tyr Ser Ala Thr Pro Asn Pro Gly Phe Gln Lys Thr Pro 
1               5                   10                  15      


Pro Leu Ala Val Lys Lys Ile Ser Gln Tyr Ala Cys Gln Arg Lys Thr 
            20                  25                  30          


Thr Leu Asn Asn Tyr Asn His Ile Phe Thr Asp Ala Phe Glu Ile Leu 
        35                  40                  45              


Ala Glu Asn Ser Glu Phe Lys Glu Asn Glu Val Ser Tyr Val Thr Phe 
    50                  55                  60                  


Met Arg Ala Ala Ser Val Leu Lys Ser Leu Pro Phe Thr Ile Ile Ser 
65                  70                  75                  80  


Met Lys Asp Thr Glu Gly Ile Pro Cys Leu Gly Asp Lys Val Lys Cys 
                85                  90                  95      


Ile Ile Glu Glu Ile Ile Glu Asp Gly Glu Ser Ser Glu Val Lys Ala 
            100                 105                 110         


Val Leu Asn Asp Glu Arg Tyr Gln Ser Phe Lys Leu Phe Thr Ser Val 
        115                 120                 125             


Phe Gly Val Gly Leu Lys Thr Ser Glu Lys Trp Phe Arg Met Gly Phe 
    130                 135                 140                 


Arg Ser Leu Ser Lys Ile Met Ser Asp Lys Thr Leu Lys Phe Thr Lys 
145                 150                 155                 160 


Met Gln Lys Ala Gly Phe Leu Tyr Tyr Glu Asp Leu Val Ser Cys Val 
                165                 170                 175     


Thr Arg Ala Glu Ala Glu Ala Val Gly Val Leu Val Lys Glu Ala Val 
            180                 185                 190         


Trp Ala Phe Leu Pro Asp Ala Phe Val Thr Met Thr Gly Gly Phe Arg 
        195                 200                 205             


Arg Gly Lys Lys Ile Gly His Asp Val Asp Phe Leu Ile Thr Ser Pro 
    210                 215                 220                 


Gly Ser Ala Glu Asp Glu Glu Gln Leu Leu Pro Lys Val Ile Asn Leu 
225                 230                 235                 240 


Trp Glu Lys Lys Gly Leu Leu Leu Tyr Tyr Asp Leu Val Glu Ser Thr 
                245                 250                 255     


Phe Glu Lys Phe Lys Leu Pro Ser Arg Gln Val Asp Thr Leu Asp His 
            260                 265                 270         


Phe Gln Lys Cys Phe Leu Ile Leu Lys Leu His His Gln Arg Val Asp 
        275                 280                 285             


Ser Ser Lys Ser Asn Gln Gln Glu Gly Lys Thr Trp Lys Ala Ile Arg 
    290                 295                 300                 


Val Asp Leu Val Met Cys Pro Tyr Glu Asn Arg Ala Phe Ala Leu Leu 
305                 310                 315                 320 


Gly Trp Thr Gly Ser Arg Gln Phe Glu Arg Asp Ile Arg Arg Tyr Ala 
                325                 330                 335     


Thr His Glu Arg Lys Met Met Leu Asp Asn His Ala Leu Tyr Asp Lys 
            340                 345                 350         


Thr Lys Arg Val Phe Leu Lys Ala Glu Ser Glu Glu Glu Ile Phe Ala 
        355                 360                 365             


His Leu Gly Leu Asp Tyr Ile Glu Pro Trp Glu Arg Asn Ala 
    370                 375                 380         


<210>  2
<211>  382
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  TdT 139-520 with Glu53Val in TdT 139-520/Glu191Val in WT


<220>
<221>  NON_TER
<222>  (1)..(1)

<400>  2

Arg Thr Asp Tyr Ser Ala Thr Pro Asn Pro Gly Phe Gln Lys Thr Pro 
1               5                   10                  15      


Pro Leu Ala Val Lys Lys Ile Ser Gln Tyr Ala Cys Gln Arg Lys Thr 
            20                  25                  30          


Thr Leu Asn Asn Tyr Asn His Ile Phe Thr Asp Ala Phe Glu Ile Leu 
        35                  40                  45              


Ala Glu Asn Ser Val Phe Lys Glu Asn Glu Val Ser Tyr Val Thr Phe 
    50                  55                  60                  


Met Arg Ala Ala Ser Val Leu Lys Ser Leu Pro Phe Thr Ile Ile Ser 
65                  70                  75                  80  


Met Lys Asp Thr Glu Gly Ile Pro Cys Leu Gly Asp Lys Val Lys Cys 
                85                  90                  95      


Ile Ile Glu Glu Ile Ile Glu Asp Gly Glu Ser Ser Glu Val Lys Ala 
            100                 105                 110         


Val Leu Asn Asp Glu Arg Tyr Gln Ser Phe Lys Leu Phe Thr Ser Val 
        115                 120                 125             


Phe Gly Val Gly Leu Lys Thr Ser Glu Lys Trp Phe Arg Met Gly Phe 
    130                 135                 140                 


Arg Ser Leu Ser Lys Ile Met Ser Asp Lys Thr Leu Lys Phe Thr Lys 
145                 150                 155                 160 


Met Gln Lys Ala Gly Phe Leu Tyr Tyr Glu Asp Leu Val Ser Cys Val 
                165                 170                 175     


Thr Arg Ala Glu Ala Glu Ala Val Gly Val Leu Val Lys Glu Ala Val 
            180                 185                 190         


Trp Ala Phe Leu Pro Asp Ala Phe Val Thr Met Thr Gly Gly Phe Arg 
        195                 200                 205             


Arg Gly Lys Lys Ile Gly His Asp Val Asp Phe Leu Ile Thr Ser Pro 
    210                 215                 220                 


Gly Ser Ala Glu Asp Glu Glu Gln Leu Leu Pro Lys Val Ile Asn Leu 
225                 230                 235                 240 


Trp Glu Lys Lys Gly Leu Leu Leu Tyr Tyr Asp Leu Val Glu Ser Thr 
                245                 250                 255     


Phe Glu Lys Phe Lys Leu Pro Ser Arg Gln Val Asp Thr Leu Asp His 
            260                 265                 270         


Phe Gln Lys Cys Phe Leu Ile Leu Lys Leu His His Gln Arg Val Asp 
        275                 280                 285             


Ser Ser Lys Ser Asn Gln Gln Glu Gly Lys Thr Trp Lys Ala Ile Arg 
    290                 295                 300                 


Val Asp Leu Val Met Cys Pro Tyr Glu Asn Arg Ala Phe Ala Leu Leu 
305                 310                 315                 320 


Gly Trp Thr Gly Ser Arg Gln Phe Glu Arg Asp Ile Arg Arg Tyr Ala 
                325                 330                 335     


Thr His Glu Arg Lys Met Met Leu Asp Asn His Ala Leu Tyr Asp Lys 
            340                 345                 350         


Thr Lys Arg Val Phe Leu Lys Ala Glu Ser Glu Glu Glu Ile Phe Ala 
        355                 360                 365             


His Leu Gly Leu Asp Tyr Ile Glu Pro Trp Glu Arg Asn Ala 
    370                 375                 380         


<210>  3
<211>  382
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  TdT 139-520 with Lys55Asn in TdT 139-520/Lys193Asn in WT


<220>
<221>  NON_TER
<222>  (1)..(1)

<400>  3

Arg Thr Asp Tyr Ser Ala Thr Pro Asn Pro Gly Phe Gln Lys Thr Pro 
1               5                   10                  15      


Pro Leu Ala Val Lys Lys Ile Ser Gln Tyr Ala Cys Gln Arg Lys Thr 
            20                  25                  30          


Thr Leu Asn Asn Tyr Asn His Ile Phe Thr Asp Ala Phe Glu Ile Leu 
        35                  40                  45              


Ala Glu Asn Ser Glu Phe Asn Glu Asn Glu Val Ser Tyr Val Thr Phe 
    50                  55                  60                  


Met Arg Ala Ala Ser Val Leu Lys Ser Leu Pro Phe Thr Ile Ile Ser 
65                  70                  75                  80  


Met Lys Asp Thr Glu Gly Ile Pro Cys Leu Gly Asp Lys Val Lys Cys 
                85                  90                  95      


Ile Ile Glu Glu Ile Ile Glu Asp Gly Glu Ser Ser Glu Val Lys Ala 
            100                 105                 110         


Val Leu Asn Asp Glu Arg Tyr Gln Ser Phe Lys Leu Phe Thr Ser Val 
        115                 120                 125             


Phe Gly Val Gly Leu Lys Thr Ser Glu Lys Trp Phe Arg Met Gly Phe 
    130                 135                 140                 


Arg Ser Leu Ser Lys Ile Met Ser Asp Lys Thr Leu Lys Phe Thr Lys 
145                 150                 155                 160 


Met Gln Lys Ala Gly Phe Leu Tyr Tyr Glu Asp Leu Val Ser Cys Val 
                165                 170                 175     


Thr Arg Ala Glu Ala Glu Ala Val Gly Val Leu Val Lys Glu Ala Val 
            180                 185                 190         


Trp Ala Phe Leu Pro Asp Ala Phe Val Thr Met Thr Gly Gly Phe Arg 
        195                 200                 205             


Arg Gly Lys Lys Ile Gly His Asp Val Asp Phe Leu Ile Thr Ser Pro 
    210                 215                 220                 


Gly Ser Ala Glu Asp Glu Glu Gln Leu Leu Pro Lys Val Ile Asn Leu 
225                 230                 235                 240 


Trp Glu Lys Lys Gly Leu Leu Leu Tyr Tyr Asp Leu Val Glu Ser Thr 
                245                 250                 255     


Phe Glu Lys Phe Lys Leu Pro Ser Arg Gln Val Asp Thr Leu Asp His 
            260                 265                 270         


Phe Gln Lys Cys Phe Leu Ile Leu Lys Leu His His Gln Arg Val Asp 
        275                 280                 285             


Ser Ser Lys Ser Asn Gln Gln Glu Gly Lys Thr Trp Lys Ala Ile Arg 
    290                 295                 300                 


Val Asp Leu Val Met Cys Pro Tyr Glu Asn Arg Ala Phe Ala Leu Leu 
305                 310                 315                 320 


Gly Trp Thr Gly Ser Arg Gln Phe Glu Arg Asp Ile Arg Arg Tyr Ala 
                325                 330                 335     


Thr His Glu Arg Lys Met Met Leu Asp Asn His Ala Leu Tyr Asp Lys 
            340                 345                 350         


Thr Lys Arg Val Phe Leu Lys Ala Glu Ser Glu Glu Glu Ile Phe Ala 
        355                 360                 365             


His Leu Gly Leu Asp Tyr Ile Glu Pro Trp Glu Arg Asn Ala 
    370                 375                 380         


<210>  4
<211>  382
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  TdT 139-520 with Glu56Gly in TdT 139-520/Glu194Gly in WT TdT


<220>
<221>  NON_TER
<222>  (1)..(1)

<400>  4

Arg Thr Asp Tyr Ser Ala Thr Pro Asn Pro Gly Phe Gln Lys Thr Pro 
1               5                   10                  15      


Pro Leu Ala Val Lys Lys Ile Ser Gln Tyr Ala Cys Gln Arg Lys Thr 
            20                  25                  30          


Thr Leu Asn Asn Tyr Asn His Ile Phe Thr Asp Ala Phe Glu Ile Leu 
        35                  40                  45              


Ala Glu Asn Ser Glu Phe Lys Gly Asn Glu Val Ser Tyr Val Thr Phe 
    50                  55                  60                  


Met Arg Ala Ala Ser Val Leu Lys Ser Leu Pro Phe Thr Ile Ile Ser 
65                  70                  75                  80  


Met Lys Asp Thr Glu Gly Ile Pro Cys Leu Gly Asp Lys Val Lys Cys 
                85                  90                  95      


Ile Ile Glu Glu Ile Ile Glu Asp Gly Glu Ser Ser Glu Val Lys Ala 
            100                 105                 110         


Val Leu Asn Asp Glu Arg Tyr Gln Ser Phe Lys Leu Phe Thr Ser Val 
        115                 120                 125             


Phe Gly Val Gly Leu Lys Thr Ser Glu Lys Trp Phe Arg Met Gly Phe 
    130                 135                 140                 


Arg Ser Leu Ser Lys Ile Met Ser Asp Lys Thr Leu Lys Phe Thr Lys 
145                 150                 155                 160 


Met Gln Lys Ala Gly Phe Leu Tyr Tyr Glu Asp Leu Val Ser Cys Val 
                165                 170                 175     


Thr Arg Ala Glu Ala Glu Ala Val Gly Val Leu Val Lys Glu Ala Val 
            180                 185                 190         


Trp Ala Phe Leu Pro Asp Ala Phe Val Thr Met Thr Gly Gly Phe Arg 
        195                 200                 205             


Arg Gly Lys Lys Ile Gly His Asp Val Asp Phe Leu Ile Thr Ser Pro 
    210                 215                 220                 


Gly Ser Ala Glu Asp Glu Glu Gln Leu Leu Pro Lys Val Ile Asn Leu 
225                 230                 235                 240 


Trp Glu Lys Lys Gly Leu Leu Leu Tyr Tyr Asp Leu Val Glu Ser Thr 
                245                 250                 255     


Phe Glu Lys Phe Lys Leu Pro Ser Arg Gln Val Asp Thr Leu Asp His 
            260                 265                 270         


Phe Gln Lys Cys Phe Leu Ile Leu Lys Leu His His Gln Arg Val Asp 
        275                 280                 285             


Ser Ser Lys Ser Asn Gln Gln Glu Gly Lys Thr Trp Lys Ala Ile Arg 
    290                 295                 300                 


Val Asp Leu Val Met Cys Pro Tyr Glu Asn Arg Ala Phe Ala Leu Leu 
305                 310                 315                 320 


Gly Trp Thr Gly Ser Arg Gln Phe Glu Arg Asp Ile Arg Arg Tyr Ala 
                325                 330                 335     


Thr His Glu Arg Lys Met Met Leu Asp Asn His Ala Leu Tyr Asp Lys 
            340                 345                 350         


Thr Lys Arg Val Phe Leu Lys Ala Glu Ser Glu Glu Glu Ile Phe Ala 
        355                 360                 365             


His Leu Gly Leu Asp Tyr Ile Glu Pro Trp Glu Arg Asn Ala 
    370                 375                 380         


<210>  5
<211>  382
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  TdT 139-520 with Asp104Tyr in TdT 139-520/Asp242Tyr in WT TdT


<220>
<221>  NON_TER
<222>  (1)..(1)

<400>  5

Arg Thr Asp Tyr Ser Ala Thr Pro Asn Pro Gly Phe Gln Lys Thr Pro 
1               5                   10                  15      


Pro Leu Ala Val Lys Lys Ile Ser Gln Tyr Ala Cys Gln Arg Lys Thr 
            20                  25                  30          


Thr Leu Asn Asn Tyr Asn His Ile Phe Thr Asp Ala Phe Glu Ile Leu 
        35                  40                  45              


Ala Glu Asn Ser Glu Phe Lys Glu Asn Glu Val Ser Tyr Val Thr Phe 
    50                  55                  60                  


Met Arg Ala Ala Ser Val Leu Lys Ser Leu Pro Phe Thr Ile Ile Ser 
65                  70                  75                  80  


Met Lys Asp Thr Glu Gly Ile Pro Cys Leu Gly Asp Lys Val Lys Cys 
                85                  90                  95      


Ile Ile Glu Glu Ile Ile Glu Tyr Gly Glu Ser Ser Glu Val Lys Ala 
            100                 105                 110         


Val Leu Asn Asp Glu Arg Tyr Gln Ser Phe Lys Leu Phe Thr Ser Val 
        115                 120                 125             


Phe Gly Val Gly Leu Lys Thr Ser Glu Lys Trp Phe Arg Met Gly Phe 
    130                 135                 140                 


Arg Ser Leu Ser Lys Ile Met Ser Asp Lys Thr Leu Lys Phe Thr Lys 
145                 150                 155                 160 


Met Gln Lys Ala Gly Phe Leu Tyr Tyr Glu Asp Leu Val Ser Cys Val 
                165                 170                 175     


Thr Arg Ala Glu Ala Glu Ala Val Gly Val Leu Val Lys Glu Ala Val 
            180                 185                 190         


Trp Ala Phe Leu Pro Asp Ala Phe Val Thr Met Thr Gly Gly Phe Arg 
        195                 200                 205             


Arg Gly Lys Lys Ile Gly His Asp Val Asp Phe Leu Ile Thr Ser Pro 
    210                 215                 220                 


Gly Ser Ala Glu Asp Glu Glu Gln Leu Leu Pro Lys Val Ile Asn Leu 
225                 230                 235                 240 


Trp Glu Lys Lys Gly Leu Leu Leu Tyr Tyr Asp Leu Val Glu Ser Thr 
                245                 250                 255     


Phe Glu Lys Phe Lys Leu Pro Ser Arg Gln Val Asp Thr Leu Asp His 
            260                 265                 270         


Phe Gln Lys Cys Phe Leu Ile Leu Lys Leu His His Gln Arg Val Asp 
        275                 280                 285             


Ser Ser Lys Ser Asn Gln Gln Glu Gly Lys Thr Trp Lys Ala Ile Arg 
    290                 295                 300                 


Val Asp Leu Val Met Cys Pro Tyr Glu Asn Arg Ala Phe Ala Leu Leu 
305                 310                 315                 320 


Gly Trp Thr Gly Ser Arg Gln Phe Glu Arg Asp Ile Arg Arg Tyr Ala 
                325                 330                 335     


Thr His Glu Arg Lys Met Met Leu Asp Asn His Ala Leu Tyr Asp Lys 
            340                 345                 350         


Thr Lys Arg Val Phe Leu Lys Ala Glu Ser Glu Glu Glu Ile Phe Ala 
        355                 360                 365             


His Leu Gly Leu Asp Tyr Ile Glu Pro Trp Glu Arg Asn Ala 
    370                 375                 380         


<210>  6
<211>  382
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  TdT 139-520 with Lys149Glu in TdT 139-520/Lys287Glu in WT TdT


<220>
<221>  NON_TER
<222>  (1)..(1)

<400>  6

Arg Thr Asp Tyr Ser Ala Thr Pro Asn Pro Gly Phe Gln Lys Thr Pro 
1               5                   10                  15      


Pro Leu Ala Val Lys Lys Ile Ser Gln Tyr Ala Cys Gln Arg Lys Thr 
            20                  25                  30          


Thr Leu Asn Asn Tyr Asn His Ile Phe Thr Asp Ala Phe Glu Ile Leu 
        35                  40                  45              


Ala Glu Asn Ser Glu Phe Lys Glu Asn Glu Val Ser Tyr Val Thr Phe 
    50                  55                  60                  


Met Arg Ala Ala Ser Val Leu Lys Ser Leu Pro Phe Thr Ile Ile Ser 
65                  70                  75                  80  


Met Lys Asp Thr Glu Gly Ile Pro Cys Leu Gly Asp Lys Val Lys Cys 
                85                  90                  95      


Ile Ile Glu Glu Ile Ile Glu Asp Gly Glu Ser Ser Glu Val Lys Ala 
            100                 105                 110         


Val Leu Asn Asp Glu Arg Tyr Gln Ser Phe Lys Leu Phe Thr Ser Val 
        115                 120                 125             


Phe Gly Val Gly Leu Lys Thr Ser Glu Lys Trp Phe Arg Met Gly Phe 
    130                 135                 140                 


Arg Ser Leu Ser Glu Ile Met Ser Asp Lys Thr Leu Lys Phe Thr Lys 
145                 150                 155                 160 


Met Gln Lys Ala Gly Phe Leu Tyr Tyr Glu Asp Leu Val Ser Cys Val 
                165                 170                 175     


Thr Arg Ala Glu Ala Glu Ala Val Gly Val Leu Val Lys Glu Ala Val 
            180                 185                 190         


Trp Ala Phe Leu Pro Asp Ala Phe Val Thr Met Thr Gly Gly Phe Arg 
        195                 200                 205             


Arg Gly Lys Lys Ile Gly His Asp Val Asp Phe Leu Ile Thr Ser Pro 
    210                 215                 220                 


Gly Ser Ala Glu Asp Glu Glu Gln Leu Leu Pro Lys Val Ile Asn Leu 
225                 230                 235                 240 


Trp Glu Lys Lys Gly Leu Leu Leu Tyr Tyr Asp Leu Val Glu Ser Thr 
                245                 250                 255     


Phe Glu Lys Phe Lys Leu Pro Ser Arg Gln Val Asp Thr Leu Asp His 
            260                 265                 270         


Phe Gln Lys Cys Phe Leu Ile Leu Lys Leu His His Gln Arg Val Asp 
        275                 280                 285             


Ser Ser Lys Ser Asn Gln Gln Glu Gly Lys Thr Trp Lys Ala Ile Arg 
    290                 295                 300                 


Val Asp Leu Val Met Cys Pro Tyr Glu Asn Arg Ala Phe Ala Leu Leu 
305                 310                 315                 320 


Gly Trp Thr Gly Ser Arg Gln Phe Glu Arg Asp Ile Arg Arg Tyr Ala 
                325                 330                 335     


Thr His Glu Arg Lys Met Met Leu Asp Asn His Ala Leu Tyr Asp Lys 
            340                 345                 350         


Thr Lys Arg Val Phe Leu Lys Ala Glu Ser Glu Glu Glu Ile Phe Ala 
        355                 360                 365             


His Leu Gly Leu Asp Tyr Ile Glu Pro Trp Glu Arg Asn Ala 
    370                 375                 380         


<210>  7
<211>  382
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  TdT 139-520 with Phe158Leu in TdT 139-520/Phe296Leu in WT TdT.


<220>
<221>  NON_TER
<222>  (1)..(1)

<400>  7

Arg Thr Asp Tyr Ser Ala Thr Pro Asn Pro Gly Phe Gln Lys Thr Pro 
1               5                   10                  15      


Pro Leu Ala Val Lys Lys Ile Ser Gln Tyr Ala Cys Gln Arg Lys Thr 
            20                  25                  30          


Thr Leu Asn Asn Tyr Asn His Ile Phe Thr Asp Ala Phe Glu Ile Leu 
        35                  40                  45              


Ala Glu Asn Ser Glu Phe Lys Glu Asn Glu Val Ser Tyr Val Thr Phe 
    50                  55                  60                  


Met Arg Ala Ala Ser Val Leu Lys Ser Leu Pro Phe Thr Ile Ile Ser 
65                  70                  75                  80  


Met Lys Asp Thr Glu Gly Ile Pro Cys Leu Gly Asp Lys Val Lys Cys 
                85                  90                  95      


Ile Ile Glu Glu Ile Ile Glu Asp Gly Glu Ser Ser Glu Val Lys Ala 
            100                 105                 110         


Val Leu Asn Asp Glu Arg Tyr Gln Ser Phe Lys Leu Phe Thr Ser Val 
        115                 120                 125             


Phe Gly Val Gly Leu Lys Thr Ser Glu Lys Trp Phe Arg Met Gly Phe 
    130                 135                 140                 


Arg Ser Leu Ser Lys Ile Met Ser Asp Lys Thr Leu Lys Leu Thr Lys 
145                 150                 155                 160 


Met Gln Lys Ala Gly Phe Leu Tyr Tyr Glu Asp Leu Val Ser Cys Val 
                165                 170                 175     


Thr Arg Ala Glu Ala Glu Ala Val Gly Val Leu Val Lys Glu Ala Val 
            180                 185                 190         


Trp Ala Phe Leu Pro Asp Ala Phe Val Thr Met Thr Gly Gly Phe Arg 
        195                 200                 205             


Arg Gly Lys Lys Ile Gly His Asp Val Asp Phe Leu Ile Thr Ser Pro 
    210                 215                 220                 


Gly Ser Ala Glu Asp Glu Glu Gln Leu Leu Pro Lys Val Ile Asn Leu 
225                 230                 235                 240 


Trp Glu Lys Lys Gly Leu Leu Leu Tyr Tyr Asp Leu Val Glu Ser Thr 
                245                 250                 255     


Phe Glu Lys Phe Lys Leu Pro Ser Arg Gln Val Asp Thr Leu Asp His 
            260                 265                 270         


Phe Gln Lys Cys Phe Leu Ile Leu Lys Leu His His Gln Arg Val Asp 
        275                 280                 285             


Ser Ser Lys Ser Asn Gln Gln Glu Gly Lys Thr Trp Lys Ala Ile Arg 
    290                 295                 300                 


Val Asp Leu Val Met Cys Pro Tyr Glu Asn Arg Ala Phe Ala Leu Leu 
305                 310                 315                 320 


Gly Trp Thr Gly Ser Arg Gln Phe Glu Arg Asp Ile Arg Arg Tyr Ala 
                325                 330                 335     


Thr His Glu Arg Lys Met Met Leu Asp Asn His Ala Leu Tyr Asp Lys 
            340                 345                 350         


Thr Lys Arg Val Phe Leu Lys Ala Glu Ser Glu Glu Glu Ile Phe Ala 
        355                 360                 365             


His Leu Gly Leu Asp Tyr Ile Glu Pro Trp Glu Arg Asn Ala 
    370                 375                 380         


<210>  8
<211>  382
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  TdT 139-520 with Met161Lys in TdT 139-520/Met299Lys in WT TdT


<220>
<221>  NON_TER
<222>  (1)..(1)

<400>  8

Arg Thr Asp Tyr Ser Ala Thr Pro Asn Pro Gly Phe Gln Lys Thr Pro 
1               5                   10                  15      


Pro Leu Ala Val Lys Lys Ile Ser Gln Tyr Ala Cys Gln Arg Lys Thr 
            20                  25                  30          


Thr Leu Asn Asn Tyr Asn His Ile Phe Thr Asp Ala Phe Glu Ile Leu 
        35                  40                  45              


Ala Glu Asn Ser Glu Phe Lys Glu Asn Glu Val Ser Tyr Val Thr Phe 
    50                  55                  60                  


Met Arg Ala Ala Ser Val Leu Lys Ser Leu Pro Phe Thr Ile Ile Ser 
65                  70                  75                  80  


Met Lys Asp Thr Glu Gly Ile Pro Cys Leu Gly Asp Lys Val Lys Cys 
                85                  90                  95      


Ile Ile Glu Glu Ile Ile Glu Asp Gly Glu Ser Ser Glu Val Lys Ala 
            100                 105                 110         


Val Leu Asn Asp Glu Arg Tyr Gln Ser Phe Lys Leu Phe Thr Ser Val 
        115                 120                 125             


Phe Gly Val Gly Leu Lys Thr Ser Glu Lys Trp Phe Arg Met Gly Phe 
    130                 135                 140                 


Arg Ser Leu Ser Lys Ile Met Ser Asp Lys Thr Leu Lys Phe Thr Lys 
145                 150                 155                 160 


Lys Gln Lys Ala Gly Phe Leu Tyr Tyr Glu Asp Leu Val Ser Cys Val 
                165                 170                 175     


Thr Arg Ala Glu Ala Glu Ala Val Gly Val Leu Val Lys Glu Ala Val 
            180                 185                 190         


Trp Ala Phe Leu Pro Asp Ala Phe Val Thr Met Thr Gly Gly Phe Arg 
        195                 200                 205             


Arg Gly Lys Lys Ile Gly His Asp Val Asp Phe Leu Ile Thr Ser Pro 
    210                 215                 220                 


Gly Ser Ala Glu Asp Glu Glu Gln Leu Leu Pro Lys Val Ile Asn Leu 
225                 230                 235                 240 


Trp Glu Lys Lys Gly Leu Leu Leu Tyr Tyr Asp Leu Val Glu Ser Thr 
                245                 250                 255     


Phe Glu Lys Phe Lys Leu Pro Ser Arg Gln Val Asp Thr Leu Asp His 
            260                 265                 270         


Phe Gln Lys Cys Phe Leu Ile Leu Lys Leu His His Gln Arg Val Asp 
        275                 280                 285             


Ser Ser Lys Ser Asn Gln Gln Glu Gly Lys Thr Trp Lys Ala Ile Arg 
    290                 295                 300                 


Val Asp Leu Val Met Cys Pro Tyr Glu Asn Arg Ala Phe Ala Leu Leu 
305                 310                 315                 320 


Gly Trp Thr Gly Ser Arg Gln Phe Glu Arg Asp Ile Arg Arg Tyr Ala 
                325                 330                 335     


Thr His Glu Arg Lys Met Met Leu Asp Asn His Ala Leu Tyr Asp Lys 
            340                 345                 350         


Thr Lys Arg Val Phe Leu Lys Ala Glu Ser Glu Glu Glu Ile Phe Ala 
        355                 360                 365             


His Leu Gly Leu Asp Tyr Ile Glu Pro Trp Glu Arg Asn Ala 
    370                 375                 380         


<210>  9
<211>  382
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  TdT 139-520 with Thr204Ser in TdT 139-520/Thr342Ser in WT TdT


<220>
<221>  NON_TER
<222>  (1)..(1)

<400>  9

Arg Thr Asp Tyr Ser Ala Thr Pro Asn Pro Gly Phe Gln Lys Thr Pro 
1               5                   10                  15      


Pro Leu Ala Val Lys Lys Ile Ser Gln Tyr Ala Cys Gln Arg Lys Thr 
            20                  25                  30          


Thr Leu Asn Asn Tyr Asn His Ile Phe Thr Asp Ala Phe Glu Ile Leu 
        35                  40                  45              


Ala Glu Asn Ser Glu Phe Lys Glu Asn Glu Val Ser Tyr Val Thr Phe 
    50                  55                  60                  


Met Arg Ala Ala Ser Val Leu Lys Ser Leu Pro Phe Thr Ile Ile Ser 
65                  70                  75                  80  


Met Lys Asp Thr Glu Gly Ile Pro Cys Leu Gly Asp Lys Val Lys Cys 
                85                  90                  95      


Ile Ile Glu Glu Ile Ile Glu Asp Gly Glu Ser Ser Glu Val Lys Ala 
            100                 105                 110         


Val Leu Asn Asp Glu Arg Tyr Gln Ser Phe Lys Leu Phe Thr Ser Val 
        115                 120                 125             


Phe Gly Val Gly Leu Lys Thr Ser Glu Lys Trp Phe Arg Met Gly Phe 
    130                 135                 140                 


Arg Ser Leu Ser Lys Ile Met Ser Asp Lys Thr Leu Lys Phe Thr Lys 
145                 150                 155                 160 


Met Gln Lys Ala Gly Phe Leu Tyr Tyr Glu Asp Leu Val Ser Cys Val 
                165                 170                 175     


Thr Arg Ala Glu Ala Glu Ala Val Gly Val Leu Val Lys Glu Ala Val 
            180                 185                 190         


Trp Ala Phe Leu Pro Asp Ala Phe Val Thr Met Ser Gly Gly Phe Arg 
        195                 200                 205             


Arg Gly Lys Lys Ile Gly His Asp Val Asp Phe Leu Ile Thr Ser Pro 
    210                 215                 220                 


Gly Ser Ala Glu Asp Glu Glu Gln Leu Leu Pro Lys Val Ile Asn Leu 
225                 230                 235                 240 


Trp Glu Lys Lys Gly Leu Leu Leu Tyr Tyr Asp Leu Val Glu Ser Thr 
                245                 250                 255     


Phe Glu Lys Phe Lys Leu Pro Ser Arg Gln Val Asp Thr Leu Asp His 
            260                 265                 270         


Phe Gln Lys Cys Phe Leu Ile Leu Lys Leu His His Gln Arg Val Asp 
        275                 280                 285             


Ser Ser Lys Ser Asn Gln Gln Glu Gly Lys Thr Trp Lys Ala Ile Arg 
    290                 295                 300                 


Val Asp Leu Val Met Cys Pro Tyr Glu Asn Arg Ala Phe Ala Leu Leu 
305                 310                 315                 320 


Gly Trp Thr Gly Ser Arg Gln Phe Glu Arg Asp Ile Arg Arg Tyr Ala 
                325                 330                 335     


Thr His Glu Arg Lys Met Met Leu Asp Asn His Ala Leu Tyr Asp Lys 
            340                 345                 350         


Thr Lys Arg Val Phe Leu Lys Ala Glu Ser Glu Glu Glu Ile Phe Ala 
        355                 360                 365             


His Leu Gly Leu Asp Tyr Ile Glu Pro Trp Glu Arg Asn Ala 
    370                 375                 380         


<210>  10
<211>  382
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  TdT 139-520 with His283Pro in TdT 139-520/His421Pro in WT TdT


<220>
<221>  NON_TER
<222>  (1)..(1)

<400>  10

Arg Thr Asp Tyr Ser Ala Thr Pro Asn Pro Gly Phe Gln Lys Thr Pro 
1               5                   10                  15      


Pro Leu Ala Val Lys Lys Ile Ser Gln Tyr Ala Cys Gln Arg Lys Thr 
            20                  25                  30          


Thr Leu Asn Asn Tyr Asn His Ile Phe Thr Asp Ala Phe Glu Ile Leu 
        35                  40                  45              


Ala Glu Asn Ser Glu Phe Lys Glu Asn Glu Val Ser Tyr Val Thr Phe 
    50                  55                  60                  


Met Arg Ala Ala Ser Val Leu Lys Ser Leu Pro Phe Thr Ile Ile Ser 
65                  70                  75                  80  


Met Lys Asp Thr Glu Gly Ile Pro Cys Leu Gly Asp Lys Val Lys Cys 
                85                  90                  95      


Ile Ile Glu Glu Ile Ile Glu Asp Gly Glu Ser Ser Glu Val Lys Ala 
            100                 105                 110         


Val Leu Asn Asp Glu Arg Tyr Gln Ser Phe Lys Leu Phe Thr Ser Val 
        115                 120                 125             


Phe Gly Val Gly Leu Lys Thr Ser Glu Lys Trp Phe Arg Met Gly Phe 
    130                 135                 140                 


Arg Ser Leu Ser Lys Ile Met Ser Asp Lys Thr Leu Lys Phe Thr Lys 
145                 150                 155                 160 


Met Gln Lys Ala Gly Phe Leu Tyr Tyr Glu Asp Leu Val Ser Cys Val 
                165                 170                 175     


Thr Arg Ala Glu Ala Glu Ala Val Gly Val Leu Val Lys Glu Ala Val 
            180                 185                 190         


Trp Ala Phe Leu Pro Asp Ala Phe Val Thr Met Thr Gly Gly Phe Arg 
        195                 200                 205             


Arg Gly Lys Lys Ile Gly His Asp Val Asp Phe Leu Ile Thr Ser Pro 
    210                 215                 220                 


Gly Ser Ala Glu Asp Glu Glu Gln Leu Leu Pro Lys Val Ile Asn Leu 
225                 230                 235                 240 


Trp Glu Lys Lys Gly Leu Leu Leu Tyr Tyr Asp Leu Val Glu Ser Thr 
                245                 250                 255     


Phe Glu Lys Phe Lys Leu Pro Ser Arg Gln Val Asp Thr Leu Asp His 
            260                 265                 270         


Phe Gln Lys Cys Phe Leu Ile Leu Lys Leu Pro His Gln Arg Val Asp 
        275                 280                 285             


Ser Ser Lys Ser Asn Gln Gln Glu Gly Lys Thr Trp Lys Ala Ile Arg 
    290                 295                 300                 


Val Asp Leu Val Met Cys Pro Tyr Glu Asn Arg Ala Phe Ala Leu Leu 
305                 310                 315                 320 


Gly Trp Thr Gly Ser Arg Gln Phe Glu Arg Asp Ile Arg Arg Tyr Ala 
                325                 330                 335     


Thr His Glu Arg Lys Met Met Leu Asp Asn His Ala Leu Tyr Asp Lys 
            340                 345                 350         


Thr Lys Arg Val Phe Leu Lys Ala Glu Ser Glu Glu Glu Ile Phe Ala 
        355                 360                 365             


His Leu Gly Leu Asp Tyr Ile Glu Pro Trp Glu Arg Asn Ala 
    370                 375                 380         


<210>  11
<211>  382
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  TdT 139-520 with TdT3-2 Substitution Mutations


<220>
<221>  NON_TER
<222>  (1)..(1)

<400>  11

Arg Thr Asp Tyr Ser Ala Thr Pro Asn Pro Gly Phe Gln Lys Thr Pro 
1               5                   10                  15      


Pro Leu Ala Val Lys Lys Ile Ser Gln Tyr Ala Cys Gln Arg Lys Thr 
            20                  25                  30          


Thr Leu Asn Asn Tyr Asn His Ile Phe Thr Asp Ala Phe Glu Ile Leu 
        35                  40                  45              


Ala Glu Asn Ser Val Phe Asn Gly Asn Glu Val Ser Tyr Val Thr Phe 
    50                  55                  60                  


Met Arg Ala Ala Ser Val Leu Lys Ser Leu Pro Phe Thr Ile Ile Ser 
65                  70                  75                  80  


Met Lys Asp Thr Glu Gly Ile Pro Cys Leu Gly Asp Lys Val Lys Cys 
                85                  90                  95      


Ile Ile Glu Glu Ile Ile Glu Tyr Gly Glu Ser Ser Glu Val Lys Ala 
            100                 105                 110         


Val Leu Asn Asp Glu Arg Tyr Gln Ser Phe Lys Leu Phe Thr Ser Val 
        115                 120                 125             


Phe Gly Val Gly Leu Lys Thr Ser Glu Lys Trp Phe Arg Met Gly Phe 
    130                 135                 140                 


Arg Ser Leu Ser Glu Ile Met Ser Asp Lys Thr Leu Lys Leu Thr Lys 
145                 150                 155                 160 


Lys Gln Lys Ala Gly Phe Leu Tyr Tyr Glu Asp Leu Val Ser Cys Val 
                165                 170                 175     


Thr Arg Ala Glu Ala Glu Ala Val Gly Val Leu Val Lys Glu Ala Val 
            180                 185                 190         


Trp Ala Phe Leu Pro Asp Ala Phe Val Thr Met Thr Gly Gly Phe Arg 
        195                 200                 205             


Arg Gly Lys Lys Ile Gly His Asp Val Asp Phe Leu Ile Thr Ser Pro 
    210                 215                 220                 


Gly Ser Ala Glu Asp Glu Glu Gln Leu Leu Pro Lys Val Ile Asn Leu 
225                 230                 235                 240 


Trp Glu Lys Lys Gly Leu Leu Leu Tyr Tyr Asp Leu Val Glu Ser Thr 
                245                 250                 255     


Phe Glu Lys Phe Lys Leu Pro Ser Arg Gln Val Asp Thr Leu Asp His 
            260                 265                 270         


Phe Gln Lys Cys Phe Leu Ile Leu Lys Leu Pro His Gln Arg Val Asp 
        275                 280                 285             


Ser Ser Lys Ser Asn Gln Gln Glu Gly Lys Thr Trp Lys Ala Ile Arg 
    290                 295                 300                 


Val Asp Leu Val Met Cys Pro Tyr Glu Asn Arg Ala Phe Ala Leu Leu 
305                 310                 315                 320 


Gly Trp Thr Gly Ser Arg Gln Phe Glu Arg Asp Ile Arg Arg Tyr Ala 
                325                 330                 335     


Thr His Glu Arg Lys Met Met Leu Asp Asn His Ala Leu Tyr Asp Lys 
            340                 345                 350         


Thr Lys Arg Val Phe Leu Lys Ala Glu Ser Glu Glu Glu Ile Phe Ala 
        355                 360                 365             


His Leu Gly Leu Asp Tyr Ile Glu Pro Trp Glu Arg Asn Ala 
    370                 375                 380         


<210>  12
<211>  520
<212>  PRT
<213>  Bos taurus

<400>  12

Met Ala Gln Gln Arg Gln His Gln Arg Leu Pro Met Asp Pro Leu Cys 
1               5                   10                  15      


Thr Ala Ser Ser Gly Pro Arg Lys Lys Arg Pro Arg Gln Val Gly Ala 
            20                  25                  30          


Ser Met Ala Ser Pro Pro His Asp Ile Lys Phe Gln Asn Leu Val Leu 
        35                  40                  45              


Phe Ile Leu Glu Lys Lys Met Gly Thr Thr Arg Arg Asn Phe Leu Met 
    50                  55                  60                  


Glu Leu Ala Arg Arg Lys Gly Phe Arg Val Glu Asn Glu Leu Ser Asp 
65                  70                  75                  80  


Ser Val Thr His Ile Val Ala Glu Asn Asn Ser Gly Ser Glu Val Leu 
                85                  90                  95      


Glu Trp Leu Gln Val Gln Asn Ile Arg Ala Ser Ser Gln Leu Glu Leu 
            100                 105                 110         


Leu Asp Val Ser Trp Leu Ile Glu Ser Met Gly Ala Gly Lys Pro Val 
        115                 120                 125             


Glu Ile Thr Gly Lys His Gln Leu Val Val Arg Thr Asp Tyr Ser Ala 
    130                 135                 140                 


Thr Pro Asn Pro Gly Phe Gln Lys Thr Pro Pro Leu Ala Val Lys Lys 
145                 150                 155                 160 


Ile Ser Gln Tyr Ala Cys Gln Arg Lys Thr Thr Leu Asn Asn Tyr Asn 
                165                 170                 175     


His Ile Phe Thr Asp Ala Phe Glu Ile Leu Ala Glu Asn Ser Glu Phe 
            180                 185                 190         


Lys Glu Asn Glu Val Ser Tyr Val Thr Phe Met Arg Ala Ala Ser Val 
        195                 200                 205             


Leu Lys Ser Leu Pro Phe Thr Ile Ile Ser Met Lys Asp Thr Glu Gly 
    210                 215                 220                 


Ile Pro Cys Leu Gly Asp Lys Val Lys Cys Ile Ile Glu Glu Ile Ile 
225                 230                 235                 240 


Glu Asp Gly Glu Ser Ser Glu Val Lys Ala Val Leu Asn Asp Glu Arg 
                245                 250                 255     


Tyr Gln Ser Phe Lys Leu Phe Thr Ser Val Phe Gly Val Gly Leu Lys 
            260                 265                 270         


Thr Ser Glu Lys Trp Phe Arg Met Gly Phe Arg Ser Leu Ser Lys Ile 
        275                 280                 285             


Met Ser Asp Lys Thr Leu Lys Phe Thr Lys Met Gln Lys Ala Gly Phe 
    290                 295                 300                 


Leu Tyr Tyr Glu Asp Leu Val Ser Cys Val Thr Arg Ala Glu Ala Glu 
305                 310                 315                 320 


Ala Val Gly Val Leu Val Lys Glu Ala Val Trp Ala Phe Leu Pro Asp 
                325                 330                 335     


Ala Phe Val Thr Met Thr Gly Gly Phe Arg Arg Gly Lys Lys Ile Gly 
            340                 345                 350         


His Asp Val Asp Phe Leu Ile Thr Ser Pro Gly Ser Ala Glu Asp Glu 
        355                 360                 365             


Glu Gln Leu Leu Pro Lys Val Ile Asn Leu Trp Glu Lys Lys Gly Leu 
    370                 375                 380                 


Leu Leu Tyr Tyr Asp Leu Val Glu Ser Thr Phe Glu Lys Phe Lys Leu 
385                 390                 395                 400 


Pro Ser Arg Gln Val Asp Thr Leu Asp His Phe Gln Lys Cys Phe Leu 
                405                 410                 415     


Ile Leu Lys Leu His His Gln Arg Val Asp Ser Ser Lys Ser Asn Gln 
            420                 425                 430         


Gln Glu Gly Lys Thr Trp Lys Ala Ile Arg Val Asp Leu Val Met Cys 
        435                 440                 445             


Pro Tyr Glu Asn Arg Ala Phe Ala Leu Leu Gly Trp Thr Gly Ser Arg 
    450                 455                 460                 


Gln Phe Glu Arg Asp Ile Arg Arg Tyr Ala Thr His Glu Arg Lys Met 
465                 470                 475                 480 


Met Leu Asp Asn His Ala Leu Tyr Asp Lys Thr Lys Arg Val Phe Leu 
                485                 490                 495     


Lys Ala Glu Ser Glu Glu Glu Ile Phe Ala His Leu Gly Leu Asp Tyr 
            500                 505                 510         


Ile Glu Pro Trp Glu Arg Asn Ala 
        515                 520 


<210>  13
<211>  98
<212>  PRT
<213>  Artificial Sequence

<220>
<223>   Saccharomyces cerevisiae SUMO 1-98


<220>
<221>  NON_TER
<222>  (98)..(98)

<400>  13

Met Ser Asp Ser Glu Val Asn Gln Glu Ala Lys Pro Glu Val Lys Pro 
1               5                   10                  15      


Glu Val Lys Pro Glu Thr His Ile Asn Leu Lys Val Ser Asp Gly Ser 
            20                  25                  30          


Ser Glu Ile Phe Phe Lys Ile Lys Lys Thr Thr Pro Leu Arg Arg Leu 
        35                  40                  45              


Met Glu Ala Phe Ala Lys Arg Gln Gly Lys Glu Met Asp Ser Leu Arg 
    50                  55                  60                  


Phe Leu Tyr Asp Gly Ile Arg Ile Gln Ala Asp Gln Thr Pro Glu Asp 
65                  70                  75                  80  


Leu Asp Met Glu Asp Asn Asp Ile Ile Glu Ala His Arg Glu Gln Ile 
                85                  90                  95      


Gly Gly 
        


<210>  14
<211>  504
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HIS-SUMO-TdT 139-520

<400>  14

Met Gly Ser Ser His His His His His His Gly Ser Gly Leu Val Pro 
1               5                   10                  15      


Arg Gly Ser Ala Ser Met Ser Asp Ser Glu Val Asn Gln Glu Ala Lys 
            20                  25                  30          


Pro Glu Val Lys Pro Glu Val Lys Pro Glu Thr His Ile Asn Leu Lys 
        35                  40                  45              


Val Ser Asp Gly Ser Ser Glu Ile Phe Phe Lys Ile Lys Lys Thr Thr 
    50                  55                  60                  


Pro Leu Arg Arg Leu Met Glu Ala Phe Ala Lys Arg Gln Gly Lys Glu 
65                  70                  75                  80  


Met Asp Ser Leu Arg Phe Leu Tyr Asp Gly Ile Arg Ile Gln Ala Asp 
                85                  90                  95      


Gln Thr Pro Glu Asp Leu Asp Met Glu Asp Asn Asp Ile Ile Glu Ala 
            100                 105                 110         


His Arg Glu Gln Ile Gly Gly Glu Leu Met Arg Thr Asp Tyr Ser Ala 
        115                 120                 125             


Thr Pro Asn Pro Gly Phe Gln Lys Thr Pro Pro Leu Ala Val Lys Lys 
    130                 135                 140                 


Ile Ser Gln Tyr Ala Cys Gln Arg Lys Thr Thr Leu Asn Asn Tyr Asn 
145                 150                 155                 160 


His Ile Phe Thr Asp Ala Phe Glu Ile Leu Ala Glu Asn Ser Glu Phe 
                165                 170                 175     


Lys Glu Asn Glu Val Ser Tyr Val Thr Phe Met Arg Ala Ala Ser Val 
            180                 185                 190         


Leu Lys Ser Leu Pro Phe Thr Ile Ile Ser Met Lys Asp Thr Glu Gly 
        195                 200                 205             


Ile Pro Cys Leu Gly Asp Lys Val Lys Cys Ile Ile Glu Glu Ile Ile 
    210                 215                 220                 


Glu Asp Gly Glu Ser Ser Glu Val Lys Ala Val Leu Asn Asp Glu Arg 
225                 230                 235                 240 


Tyr Gln Ser Phe Lys Leu Phe Thr Ser Val Phe Gly Val Gly Leu Lys 
                245                 250                 255     


Thr Ser Glu Lys Trp Phe Arg Met Gly Phe Arg Ser Leu Ser Lys Ile 
            260                 265                 270         


Met Ser Asp Lys Thr Leu Lys Phe Thr Lys Met Gln Lys Ala Gly Phe 
        275                 280                 285             


Leu Tyr Tyr Glu Asp Leu Val Ser Cys Val Thr Arg Ala Glu Ala Glu 
    290                 295                 300                 


Ala Val Gly Val Leu Val Lys Glu Ala Val Trp Ala Phe Leu Pro Asp 
305                 310                 315                 320 


Ala Phe Val Thr Met Thr Gly Gly Phe Arg Arg Gly Lys Lys Ile Gly 
                325                 330                 335     


His Asp Val Asp Phe Leu Ile Thr Ser Pro Gly Ser Ala Glu Asp Glu 
            340                 345                 350         


Glu Gln Leu Leu Pro Lys Val Ile Asn Leu Trp Glu Lys Lys Gly Leu 
        355                 360                 365             


Leu Leu Tyr Tyr Asp Leu Val Glu Ser Thr Phe Glu Lys Phe Lys Leu 
    370                 375                 380                 


Pro Ser Arg Gln Val Asp Thr Leu Asp His Phe Gln Lys Cys Phe Leu 
385                 390                 395                 400 


Ile Leu Lys Leu His His Gln Arg Val Asp Ser Ser Lys Ser Asn Gln 
                405                 410                 415     


Gln Glu Gly Lys Thr Trp Lys Ala Ile Arg Val Asp Leu Val Met Cys 
            420                 425                 430         


Pro Tyr Glu Asn Arg Ala Phe Ala Leu Leu Gly Trp Thr Gly Ser Arg 
        435                 440                 445             


Gln Phe Glu Arg Asp Ile Arg Arg Tyr Ala Thr His Glu Arg Lys Met 
    450                 455                 460                 


Met Leu Asp Asn His Ala Leu Tyr Asp Lys Thr Lys Arg Val Phe Leu 
465                 470                 475                 480 


Lys Ala Glu Ser Glu Glu Glu Ile Phe Ala His Leu Gly Leu Asp Tyr 
                485                 490                 495     


Ile Glu Pro Trp Glu Arg Asn Ala 
            500                 


<210>  15
<211>  504
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  TdT3-2

<400>  15

Met Gly Ser Ser His His His His His His Gly Ser Gly Leu Val Pro 
1               5                   10                  15      


Arg Gly Ser Ala Ser Met Ser Asp Ser Glu Val Asn Gln Glu Ala Lys 
            20                  25                  30          


Pro Glu Val Lys Pro Glu Val Lys Pro Glu Thr His Ile Asn Leu Lys 
        35                  40                  45              


Val Ser Asp Gly Ser Ser Glu Ile Phe Phe Lys Ile Lys Lys Thr Thr 
    50                  55                  60                  


Pro Leu Arg Arg Leu Met Glu Ala Phe Ala Lys Arg Gln Gly Lys Glu 
65                  70                  75                  80  


Met Asp Ser Leu Arg Phe Leu Tyr Asp Gly Ile Arg Ile Gln Ala Asp 
                85                  90                  95      


Gln Thr Pro Glu Asp Leu Asp Met Glu Asp Asn Asp Ile Ile Glu Ala 
            100                 105                 110         


His Arg Glu Gln Ile Gly Gly Glu Leu Met Arg Thr Asp Tyr Ser Ala 
        115                 120                 125             


Thr Pro Asn Pro Gly Phe Gln Lys Thr Pro Pro Leu Ala Val Lys Lys 
    130                 135                 140                 


Ile Ser Gln Tyr Ala Cys Gln Arg Lys Thr Thr Leu Asn Asn Tyr Asn 
145                 150                 155                 160 


His Ile Phe Thr Asp Ala Phe Glu Ile Leu Ala Glu Asn Ser Val Phe 
                165                 170                 175     


Asn Gly Asn Glu Val Ser Tyr Val Thr Phe Met Arg Ala Ala Ser Val 
            180                 185                 190         


Leu Lys Ser Leu Pro Phe Thr Ile Ile Ser Met Lys Asp Thr Glu Gly 
        195                 200                 205             


Ile Pro Cys Leu Gly Asp Lys Val Lys Cys Ile Ile Glu Glu Ile Ile 
    210                 215                 220                 


Glu Tyr Gly Glu Ser Ser Glu Val Lys Ala Val Leu Asn Asp Glu Arg 
225                 230                 235                 240 


Tyr Gln Ser Phe Lys Leu Phe Thr Ser Val Phe Gly Val Gly Leu Lys 
                245                 250                 255     


Thr Ser Glu Lys Trp Phe Arg Met Gly Phe Arg Ser Leu Ser Glu Ile 
            260                 265                 270         


Met Ser Asp Lys Thr Leu Lys Leu Thr Lys Lys Gln Lys Ala Gly Phe 
        275                 280                 285             


Leu Tyr Tyr Glu Asp Leu Val Ser Cys Val Thr Arg Ala Glu Ala Glu 
    290                 295                 300                 


Ala Val Gly Val Leu Val Lys Glu Ala Val Trp Ala Phe Leu Pro Asp 
305                 310                 315                 320 


Ala Phe Val Thr Met Thr Gly Gly Phe Arg Arg Gly Lys Lys Ile Gly 
                325                 330                 335     


His Asp Val Asp Phe Leu Ile Thr Ser Pro Gly Ser Ala Glu Asp Glu 
            340                 345                 350         


Glu Gln Leu Leu Pro Lys Val Ile Asn Leu Trp Glu Lys Lys Gly Leu 
        355                 360                 365             


Leu Leu Tyr Tyr Asp Leu Val Glu Ser Thr Phe Glu Lys Phe Lys Leu 
    370                 375                 380                 


Pro Ser Arg Gln Val Asp Thr Leu Asp His Phe Gln Lys Cys Phe Leu 
385                 390                 395                 400 


Ile Leu Lys Leu Pro His Gln Arg Val Asp Ser Ser Lys Ser Asn Gln 
                405                 410                 415     


Gln Glu Gly Lys Thr Trp Lys Ala Ile Arg Val Asp Leu Val Met Cys 
            420                 425                 430         


Pro Tyr Glu Asn Arg Ala Phe Ala Leu Leu Gly Trp Thr Gly Ser Arg 
        435                 440                 445             


Gln Phe Glu Arg Asp Ile Arg Arg Tyr Ala Thr His Glu Arg Lys Met 
    450                 455                 460                 


Met Leu Asp Asn His Ala Leu Tyr Asp Lys Thr Lys Arg Val Phe Leu 
465                 470                 475                 480 


Lys Ala Glu Ser Glu Glu Glu Ile Phe Ala His Leu Gly Leu Asp Tyr 
                485                 490                 495     


Ile Glu Pro Trp Glu Arg Asn Ala 
            500                 


<210>  16
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Oligonucleotide


<220>
<221>  modified_base
<222>  (19)..(19)
<223>  Fluorescein T

<400>  16
cgcttgcaca ggtgcgtttc a                                                 21


<210>  17
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Oligonucleotide


<220>
<221>  misc_difference
<222>  (1)..(1)
<223>  5' 6-Fluorescein

<400>  17
cgcttgcaca ggtgcgttcg                                                   20


<210>  18
<211>  43
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Oligonucleotide

<400>  18
aaaaaacacc tgcggcctgg tgccgcgcgg cagcgctagc atg                         43


<210>  19
<211>  37
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Oligonucleotide

<400>  19
aaaaaacacc tgcgggatcc ggtaccgcgg ccgccta                                37


<210>  20
<211>  35
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Oligonucleotide

<400>  20
attctgtgtt taawgraaat gaagtctctt atgtg                                  35


<210>  21
<211>  38
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Oligonucleotide

<400>  21
agatctctga gtraaataat gtcagacaaa accctgaa                               38


<210>  22
<211>  41
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Oligonucleotide

<400>  22
agacaaaacc ctgaaattma caaaaawgca gaaagcagga t                           41


<210>  23
<211>  29
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Oligonucleotide

<400>  23
tttgtcacca tgwcaggagg attccgcag                                         29


<210>  24
<211>  34
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Oligonucleotide

<400>  24
gattttaaaa ttgcmccatc agagagtaga cagt                                   34


<210>  25
<211>  35
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Oligonucleotide

<400>  25
tctagagccc gcctaatcag cgggcttttt tttat                                  35


<210>  26
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Oligonucleotide


<220>
<221>  misc_difference
<222>  (1)..(1)
<223>  5' 6-Fluorescein

<400>  26
attcaggacg agcctcagac c                                                 21


<210>  27
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Oligonucleotide


<220>
<221>  misc_difference
<222>  (1)..(1)
<223>  5' 6-Fluorescein

<400>  27
tttcggtggt cgccgtatcc gc                                                22


<210>  28
<211>  35
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Oligonucleotide


<220>
<221>  misc_difference
<222>  (35)..(35)
<223>  3' Phosphate

<400>  28
gcggatacgg cgaccaccga gatctacact ctgag                                  35


<210>  29
<211>  38
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Oligonucleotide


<220>
<221>  misc_difference
<222>  (38)..(38)
<223>  3' Phosphate

<400>  29
tttctcagag tgtagatctc ggtggtcgcc gtatccgc                               38


<210>  30
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Oligonucleotide


<220>
<221>  modified_base
<222>  (20)..(20)
<223>  Fluorescein T

<400>  30
cgcttgcaca ggtgcgttct a                                                 21


<210>  31
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Oligonucleotide


<220>
<221>  modified_base
<222>  (16)..(16)
<223>  Fluorescein T

<400>  31
cgcttgcaca ggtgctgttc a                                                 21


<210>  32
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Oligonucleotide


<220>
<221>  modified_base
<222>  (11)..(11)
<223>  Fluorescein T

<400>  32
cgcttgcaca tggtgcgttc a                                                 21


<210>  33
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Oligonucleotide


<220>
<221>  misc_difference
<222>  (17)..(18)
<223>  T Cy3 T

<400>  33
cgcttgcaca ggtgcgttca                                                   20


