                         SEQUENCE LISTING

<110>  The Regents of the University of Colorado, a body corporate
 
<120>  COMPOSITIONS, METHODS AND USES FOR QUANTIFYING TRANSCRIPTION AND 
       BIOSENSING OF SMALL MOLECULES USING A NOVEL TYPE VI CRISPR-CAS 
       ASSAY

<130>  101877-688452 (CU5268B-PCT1)

<150>  63/023,063
<151>  2020-05-11

<160>  45    

<170>  PatentIn version 3.5

<210>  1
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic construct - primer

<400>  1
gctagccaca gctaacacca cgtc                                              24


<210>  2
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic construct - primer

<400>  2
ctttatgctt ccggctcgta tgttgtg                                           27


<210>  3
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic construct - primer

<400>  3
ctaaggatga tttctggaat tc                                                22


<210>  4
<211>  31
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic construct - primer

<400>  4
atttatcaaa acatttaagt aaaggagttt g                                      31


<210>  5
<211>  34
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic construct - primer

<400>  5
aatagctatt taatttgaat atattatacg agcc                                   34


<210>  6
<211>  15
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic construct - primer

<400>  6
cgcttctgcg ttctg                                                        15


<210>  7
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic construct - primer

<400>  7
ggccagtgaa ttcgagctcg                                                   20


<210>  8
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic construct - primer

<400>  8
gaatatatta tacgagcctt atgcatg                                           27


<210>  9
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic construct - primer

<400>  9
gaagcttggg cccgaacaaa aac                                               23


<210>  10
<211>  26
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic construct - primer

<400>  10
agatctttag aattccagaa atcatc                                            26


<210>  11
<211>  31
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic construct - primer

<400>  11
gatcctctag aaatatggat tacttggtag g                                      31


<210>  12
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic construct - primer

<400>  12
ggcggcgaat tctaatacga ctcactatag                                        30


<210>  13
<211>  60
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic construct - primer

<400>  13
gcataaggct cgtataatat attccaaaaa attaataaca ttttctctta tcgagagttg       60


<210>  14
<211>  37
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic construct - primer

<400>  14
ctcatgggaa agaggctttt tggccagtga attcgag                                37


<210>  15
<211>  35
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic construct - tac promoter

<400>  15
ttgacaggca tgcataaggc tcgtataata tattc                                  35


<210>  16
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic construct - T7 promoter

<400>  16
taatacgact cactatagg                                                    19


<210>  17
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic construct - Cas13a target site

<400>  17
gatcctctag aaatatggat tacttggtag                                        30


<210>  18
<211>  66
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic construct - crRNA

<400>  18
gatttagact accccaaaaa cgaaggggac taaaacctac caagtaatcc atatttctag       60

aggatc                                                                  66


<210>  19
<211>  180
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic construct - guanine riboswitch

<400>  19
aattaaatag ctattatcac gatttttata atcgcgtgga tatggcacgc aagtttctac       60

cgggcaccgt aaatgtccga ctaaaaatcc tgattacaaa atttgtttat gacatttttt      120

gtaatcagga ttttttattt atcaaaacat ttaagtaaag gagtttgtta tgtttttttt      180


<210>  20
<211>  57
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic construct - guanine aptamer

<400>  20
tataatcgcg tggatatggc acgcaagttt ctaccgggca ccgtaaatgt ccgacta          57


<210>  21
<211>  253
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic construct -  FMN riboswitch

<400>  21
aattaaatag ctattatcac gatttttcgg ggcagggtgg aaatcccgac cggcggtagt       60

aaagcacatt tgctttagag cccgtgaccc gtgtgcataa gcacgcggtg gattcagttt      120

aagctgaagc cgacagtgaa agtctggatg ggagaaaaat cctgattaca aaatttgttt      180

atgacatttt ttgtaatcag gattttttta tttatcaaaa catttaagta aaggagtttg      240

ttatgttttt ttt                                                         253


<210>  22
<211>  127
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic construct - FMN aptamer

<400>  22
cggggcaggg tggaaatccc gaccggcggt agtaaagcac atttgcttta gagcccgtga       60

cccgtgtgca taagcacgcg gtggattcag tttaagctga agccgacagt gaaagtctgg      120

atgggag                                                                127


<210>  23
<211>  231
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic construct - SAM riboswitch

<400>  23
aattaaatag ctattatcac gattttatca agagaagcag agggactggc ccgacgaagc       60

ttcagcaacc ggtgtaatgg cgatcagcca tgaccaaggt gctaaatcca gcaagctcga      120

acagcttgga agataaaatc ctgattacaa aatttgttta tgacattttt tgtaatcagg      180

attttttatt tatcaaaaca tttaagtaaa ggagtttgtt atgttttttt t               231


<210>  24
<211>  108
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic construct - SAM aptamer

<400>  24
atcaagagaa gcagagggac tggcccgacg aagcttcagc aaccggtgta atggcgatca       60

gccatgacca aggtgctaaa tccagcaagc tcgaacagct tggaagat                   108


<210>  25
<211>  100
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic construct - adenine riboswitch

<400>  25
cacttgtata acctcaataa tatggtttga gggtgtctac caggaaccgt aaaatcctga       60

ttacaagccg ttttttcggc ttgtaatcag gatttttttt                            100


<210>  26
<211>  57
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic construct - adenine aptamer

<400>  26
tataacctca ataatatggt ttgagggtgt ctaccaggaa ccgtaaaatc ctgatta          57


<210>  27
<211>  79
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic construct - fluoride riboswitch

<400>  27
taggcgatgg agttcgccat aaacgctgct tagctaatga ctcctaccag tatcactact       60

ggtaggagtc tattttttt                                                    79


<210>  28
<211>  229
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic construct - SAM riboswitch

<400>  28
caaaaaatta ataacatttt ctcttatcga gagttgggcg agggattggc cttttgaccc       60

caacagcaac cgaccgtaat accattgtga aatggggcgc actgcttttc gcgccgagac      120

tgatgtctca taaggcacgg tgctaattcc atcagattgt gtctgagaga tgagagaggc      180

agtgttttac gtagaaaagc ctctttctct catgggaaag aggcttttt                  229


<210>  29
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic construct - zinc aTF operator

<400>  29
cacatgaaca gttattcaga ta                                                22


<210>  30
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic construct - tetracycline aTF operator

<400>  30
tccctatcag tgatagaga                                                    19


<210>  31
<211>  104
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic construct

<400>  31
gctagccaca gctaacacca cgtcgtccct atctgctgcc ctaggtctat gagtggttgc       60

tggataactt gacaggcatg cataaggctc gtataatata ttca                       104


<210>  32
<211>  52
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic construct

<400>  32
ctaaggatga tttctggaat tctaaagatc ttaatacgac tcactatagg ga               52


<210>  33
<211>  173
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic construct

<400>  33
ggccagtgaa ttcgagctcg gtacccgggg atcctctaga aatatggatt acttggtaga       60

acagcaatct actcgacctg caggcatgca agcttggcgt aatcatggtc atagctgttt      120

cctgtgttta tccgctcaca attccacaca acatacgagc cggaagcata aag             173


<210>  34
<211>  2905
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic construct - plasmid

<400>  34
gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat       60

cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa      120

ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa      180

gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga      240

taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg      300

gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc      360

acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg      420

aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact      480

cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat      540

atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt      600

gccacctgac gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat      660

cacgaggcag aatttcagat aaaaaaaatc cttagctttc gctaaggatg atttctggaa      720

ttctaaagat ctgcgctagc cacagctaac accacgtcgt ccctatctgc tgccctaggt      780

ctatgagtgg ttgctggata acttgacagg catgcataag gctcgtataa tatattcaaa      840

ttaaatagct attatcacga tttttataat cgcgtggata tggcacgcaa gtttctaccg      900

ggcaccgtaa atgtccgact aaaaatcctg attacaaaat ttgtttatga cattttttgt      960

aatcaggatt ttttatttat caaaacattt aagtaaagga gtttgttatg ttttttttgg     1020

ccagtgaatt cgagctcggt acccggggat cctctagaaa tatggattac ttggtagaac     1080

agcaatctac tcgacctgca ggcatgcaag cttggcgtaa tcatggtcat agctgtttcc     1140

tgtgtttatc cgctcacaat tccacacaac atacgagccg gaagcataaa ggaagcttgg     1200

gcccgaacaa aaactcatct cagaagagga tctgaatagc gccgtcgacc atcatcatca     1260

tcatcattga gtttaaacgg tctccagctt ggctgttttg gcggatgaga gaagattttc     1320

agcctgatac agattaaatc agaacgcaga agcggtctga taaaacagaa tttgcctggc     1380

ggcagtagcg cggtggtccc acctgacccc atgccgaact cagaagtgaa acgccgtagc     1440

gccgatggta gtgtggggtc tccccatgcg agagtaggga actgccaggc atcaaataaa     1500

acgaaaggct cagtcgaaag actgggcctt tcgttttatc tgttgtttgt cggtgaactg     1560

gatccttact cgagtctaga ctgcaggctt cctcgctcac tgactcgctg cgctcggtcg     1620

ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat     1680

caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta     1740

aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa     1800

atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc     1860

cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt     1920

ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca     1980

gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg     2040

accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat     2100

cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta     2160

cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct     2220

gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac     2280

aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa     2340

aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa     2400

actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt     2460

taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca     2520

gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca     2580

tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc     2640

ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa     2700

accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc     2760

agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca     2820

acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat     2880

tcagctccgg ttcccaacga tcaag                                           2905


<210>  35
<211>  101
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic construct

<400>  35
ggcggcgaat tctaatacga ctcactatag gggaagattt agactacccc aaaaacgaag       60

gggactaaaa cctaccaagt aatccatatt tctagaggat c                          101


<210>  36
<211>  276
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic construct

<400>  36
gctagccaca gctaacacca cgtcgtccct atctgctgcc ctaggtctat gagtggttgc       60

tggataactt gacaggcatg cataaggctc gtataatata ttcggccagt gaattcgagc      120

tcggtacccg gggatcctct agaaatatgg attacttggt agaacagcaa tctactcgac      180

ctgcaggcat gcaagcttgg cgtaatcatg gtcatagctg tttcctgtgt ttatccgctc      240

acaattccac acaacatacg agccggaagc ataaag                                276


<210>  37
<211>  457
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic construct

<400>  37
gctagccaca gctaacacca cgtcgtccct atctgctgcc ctaggtctat gagtggttgc       60

tggataactt gacaggcatg cataaggctc gtataatata ttcaaattaa atagctatta      120

tcacgatttt tataatcgcg tggatatggc acgcaagttt ctaccgggca ccgtaaatgt      180

ccgactaaaa atcctgatta caaaatttgt ttatgacatt ttttgtaatc aggatttttt      240

atttatcaaa acatttaagt aaaggagttt gttatgtttt ttttggccag tgaattcgag      300

ctcggtaccc ggggatcctc tagaaatatg gattacttgg tagaacagca atctactcga      360

cctgcaggca tgcaagcttg gcgtaatcat ggtcatagct gtttcctgtg tttatccgct      420

cacaattcca cacaacatac gagccggaag cataaag                               457


<210>  38
<211>  530
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic construct

<400>  38
gctagccaca gctaacacca cgtcgtccct atctgctgcc ctaggtctat gagtggttgc       60

tggataactt gacaggcatg cataaggctc gtataatata ttcaaattaa atagctatta      120

tcacgatttt tcggggcagg gtggaaatcc cgaccggcgg tagtaaagca catttgcttt      180

agagcccgtg acccgtgtgc ataagcacgc ggtggattca gtttaagctg aagccgacag      240

tgaaagtctg gatgggagaa aaatcctgat tacaaaattt gtttatgaca ttttttgtaa      300

tcaggatttt tttatttatc aaaacattta agtaaaggag tttgttatgt tttttttggc      360

cagtgaattc gagctcggta cccggggatc ctctagaaat atggattact tggtagaaca      420

gcaatctact cgacctgcag gcatgcaagc ttggcgtaat catggtcata gctgtttcct      480

gtgtttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag                 530


<210>  39
<211>  508
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic construct

<400>  39
gctagccaca gctaacacca cgtcgtccct atctgctgcc ctaggtctat gagtggttgc       60

tggataactt gacaggcatg cataaggctc gtataatata ttcaaattaa atagctatta      120

tcacgatttt atcaagagaa gcagagggac tggcccgacg aagcttcagc aaccggtgta      180

atggcgatca gccatgacca aggtgctaaa tccagcaagc tcgaacagct tggaagataa      240

aatcctgatt acaaaatttg tttatgacat tttttgtaat caggattttt tatttatcaa      300

aacatttaag taaaggagtt tgttatgttt tttttggcca gtgaattcga gctcggtacc      360

cggggatcct ctagaaatat ggattacttg gtagaacagc aatctactcg acctgcaggc      420

atgcaagctt ggcgtaatca tggtcatagc tgtttcctgt gtttatccgc tcacaattcc      480

acacaacata cgagccggaa gcataaag                                         508


<210>  40
<211>  377
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic construct

<400>  40
gctagccaca gctaacacca cgtcgtccct atctgctgcc ctaggtctat gagtggttgc       60

tggataactt gacaggcatg cataaggctc gtataatata ttcacacttg tataacctca      120

ataatatggt ttgagggtgt ctaccaggaa ccgtaaaatc ctgattacaa gccgtttttt      180

cggcttgtaa tcaggatttt ttttggccag tgaattcgag ctcggtaccc ggggatcctc      240

tagaaatatg gattacttgg tagaacagca atctactcga cctgcaggca tgcaagcttg      300

gcgtaatcat ggtcatagct gtttcctgtg tttatccgct cacaattcca cacaacatac      360

gagccggaag cataaag                                                     377


<210>  41
<211>  355
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic construct

<400>  41
gctagccaca gctaacacca cgtcgtccct atctgctgcc ctaggtctat gagtggttgc       60

tggataactt gacaggcatg cataaggctc gtataatata ttctaggcga tggagttcgc      120

cataaacgct gcttagctaa tgactcctac cagtatcact actggtagga gtctattttt      180

ttggccagtg aattcgagct cggtacccgg ggatcctcta gaaatatgga ttacttggta      240

gaacagcaat ctactcgacc tgcaggcatg caagcttggc gtaatcatgg tcatagctgt      300

ttcctgtgtt tatccgctca caattccaca caacatacga gccggaagca taaag           355


<210>  42
<211>  505
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic construct

<400>  42
gctagccaca gctaacacca cgtcgtccct atctgctgcc ctaggtctat gagtggttgc       60

tggataactt gacaggcatg cataaggctc gtataatata ttccaaaaaa ttaataacat      120

tttctcttat cgagagttgg gcgagggatt ggccttttga ccccaacagc aaccgaccgt      180

aataccattg tgaaatgggg cgcactgctt ttcgcgccga gactgatgtc tcataaggca      240

cggtgctaat tccatcagat tgtgtctgag agatgagaga ggcagtgttt tacgtagaaa      300

agcctctttc tctcatggga aagaggcttt ttggccagtg aattcgagct cggtacccgg      360

ggatcctcta gaaatatgga ttacttggta gaacagcaat ctactcgacc tgcaggcatg      420

caagcttggc gtaatcatgg tcatagctgt ttcctgtgtt tatccgctca caattccaca      480

caacatacga gccggaagca taaag                                            505


<210>  43
<211>  223
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic construct

<400>  43
ctaaggatga tttctggaat tctaaagatc ttaatacgac tcactatagg ggccagtgaa       60

ttcgagctcg gtacccgggg atcctctaga aatatggatt acttggtaga acagcaatct      120

actcgacctg caggcatgca agcttggcgt aatcatggtc atagctgttt cctgtgttta      180

tccgctcaca attccacaca acatacgagc cggaagcata aag                        223


<210>  44
<211>  247
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic construct

<400>  44
ctaaggatga tttctggaat tctaaagatc ttaatacgac tcactatagg gacacatgaa       60

cagttattca gataggccag tgaattcgag ctcggtaccc ggggatcctc tagaaatatg      120

gattacttgg tagaacagca atctactcga cctgcaggca tgcaagcttg gcgtaatcat      180

ggtcatagct gtttcctgtg tttatccgct cacaattcca cacaacatac gagccggaag      240

cataaag                                                                247


<210>  45
<211>  244
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic construct

<400>  45
ctaaggatga tttctggaat tctaaagatc ttaatacgac tcactatagg gatccctatc       60

agtgatagag aggccagtga attcgagctc ggtacccggg gatcctctag aaatatggat      120

tacttggtag aacagcaatc tactcgacct gcaggcatgc aagcttggcg taatcatggt      180

catagctgtt tcctgtgttt atccgctcac aattccacac aacatacgag ccggaagcat      240

aaag                                                                   244


