                         SEQUENCE LISTING

<110>  The Trustees of the University of Pennsylvania
 
<120>  COMPOSITIONS FOR TREATMENT OF KRABBE DISEASE

<130>  21-9660PCT

<150>  US 63/023,459
<151>  2020-05-12

<150>  US 63/070,653
<151>  2020-08-26

<150>  US 63/073,756
<151>  2020-09-02

<160>  27    

<170>  PatentIn version 3.5

<210>  1
<211>  2211
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  AAVhu68 vp1 capsid


<220>
<221>  CDS
<222>  (1)..(2211)

<400>  1
atg gct gcc gat ggt tat ctt cca gat tgg ctc gag gac aac ctc agt         48
Met Ala Ala Asp Gly Tyr Leu Pro Asp Trp Leu Glu Asp Asn Leu Ser           
1               5                   10                  15                

gaa ggc att cgc gag tgg tgg gct ttg aaa cct gga gcc cct caa ccc         96
Glu Gly Ile Arg Glu Trp Trp Ala Leu Lys Pro Gly Ala Pro Gln Pro           
            20                  25                  30                    

aag gca aat caa caa cat caa gac aac gct cgg ggt ctt gtg ctt ccg        144
Lys Ala Asn Gln Gln His Gln Asp Asn Ala Arg Gly Leu Val Leu Pro           
        35                  40                  45                        

ggt tac aaa tac ctt gga ccc ggc aac gga ctc gac aag ggg gag ccg        192
Gly Tyr Lys Tyr Leu Gly Pro Gly Asn Gly Leu Asp Lys Gly Glu Pro           
    50                  55                  60                            

gtc aac gaa gca gac gcg gcg gcc ctc gag cac gac aag gcc tac gac        240
Val Asn Glu Ala Asp Ala Ala Ala Leu Glu His Asp Lys Ala Tyr Asp           
65                  70                  75                  80            

cag cag ctc aag gcc gga gac aac ccg tac ctc aag tac aac cac gcc        288
Gln Gln Leu Lys Ala Gly Asp Asn Pro Tyr Leu Lys Tyr Asn His Ala           
                85                  90                  95                

gac gcc gag ttc cag gag cgg ctc aaa gaa gat acg tct ttt ggg ggc        336
Asp Ala Glu Phe Gln Glu Arg Leu Lys Glu Asp Thr Ser Phe Gly Gly           
            100                 105                 110                   

aac ctc ggg cga gca gtc ttc cag gcc aaa aag agg ctt ctt gaa cct        384
Asn Leu Gly Arg Ala Val Phe Gln Ala Lys Lys Arg Leu Leu Glu Pro           
        115                 120                 125                       

ctt ggt ctg gtt gag gaa gcg gct aag acg gct cct gga aag aag agg        432
Leu Gly Leu Val Glu Glu Ala Ala Lys Thr Ala Pro Gly Lys Lys Arg           
    130                 135                 140                           

cct gta gag cag tct cct cag gaa ccg gac tcc tcc gtg ggt att ggc        480
Pro Val Glu Gln Ser Pro Gln Glu Pro Asp Ser Ser Val Gly Ile Gly           
145                 150                 155                 160           

aaa tcg ggt gca cag ccc gct aaa aag aga ctc aat ttc ggt cag act        528
Lys Ser Gly Ala Gln Pro Ala Lys Lys Arg Leu Asn Phe Gly Gln Thr           
                165                 170                 175               

ggc gac aca gag tca gtc ccc gac cct caa cca atc gga gaa cct ccc        576
Gly Asp Thr Glu Ser Val Pro Asp Pro Gln Pro Ile Gly Glu Pro Pro           
            180                 185                 190                   

gca gcc ccc tca ggt gtg gga tct ctt aca atg gct tca ggt ggt ggc        624
Ala Ala Pro Ser Gly Val Gly Ser Leu Thr Met Ala Ser Gly Gly Gly           
        195                 200                 205                       

gca cca gtg gca gac aat aac gaa ggt gcc gat gga gtg ggt agt tcc        672
Ala Pro Val Ala Asp Asn Asn Glu Gly Ala Asp Gly Val Gly Ser Ser           
    210                 215                 220                           

tcg gga aat tgg cat tgc gat tcc caa tgg ctg ggg gac aga gtc atc        720
Ser Gly Asn Trp His Cys Asp Ser Gln Trp Leu Gly Asp Arg Val Ile           
225                 230                 235                 240           

acc acc agc acc cga acc tgg gcc ctg ccc acc tac aac aat cac ctc        768
Thr Thr Ser Thr Arg Thr Trp Ala Leu Pro Thr Tyr Asn Asn His Leu           
                245                 250                 255               

tac aag caa atc tcc aac agc aca tct gga gga tct tca aat gac aac        816
Tyr Lys Gln Ile Ser Asn Ser Thr Ser Gly Gly Ser Ser Asn Asp Asn           
            260                 265                 270                   

gcc tac ttc ggc tac agc acc ccc tgg ggg tat ttt gac ttc aac aga        864
Ala Tyr Phe Gly Tyr Ser Thr Pro Trp Gly Tyr Phe Asp Phe Asn Arg           
        275                 280                 285                       

ttc cac tgc cac ttc tca cca cgt gac tgg caa aga ctc atc aac aac        912
Phe His Cys His Phe Ser Pro Arg Asp Trp Gln Arg Leu Ile Asn Asn           
    290                 295                 300                           

aac tgg gga ttc cgg cct aag cga ctc aac ttc aag ctc ttc aac att        960
Asn Trp Gly Phe Arg Pro Lys Arg Leu Asn Phe Lys Leu Phe Asn Ile           
305                 310                 315                 320           

cag gtc aaa gag gtt acg gac aac aat gga gtc aag acc atc gct aat       1008
Gln Val Lys Glu Val Thr Asp Asn Asn Gly Val Lys Thr Ile Ala Asn           
                325                 330                 335               

aac ctt acc agc acg gtc cag gtc ttc acg gac tca gac tat cag ctc       1056
Asn Leu Thr Ser Thr Val Gln Val Phe Thr Asp Ser Asp Tyr Gln Leu           
            340                 345                 350                   

ccg tac gtg ctc ggg tcg gct cac gag ggc tgc ctc ccg ccg ttc cca       1104
Pro Tyr Val Leu Gly Ser Ala His Glu Gly Cys Leu Pro Pro Phe Pro           
        355                 360                 365                       

gcg gac gtt ttc atg att cct cag tac ggg tat cta acg ctt aat gat       1152
Ala Asp Val Phe Met Ile Pro Gln Tyr Gly Tyr Leu Thr Leu Asn Asp           
    370                 375                 380                           

gga agc caa gcc gtg ggt cgt tcg tcc ttt tac tgc ctg gaa tat ttc       1200
Gly Ser Gln Ala Val Gly Arg Ser Ser Phe Tyr Cys Leu Glu Tyr Phe           
385                 390                 395                 400           

ccg tcg caa atg cta aga acg ggt aac aac ttc cag ttc agc tac gag       1248
Pro Ser Gln Met Leu Arg Thr Gly Asn Asn Phe Gln Phe Ser Tyr Glu           
                405                 410                 415               

ttt gag aac gta cct ttc cat agc agc tat gct cac agc caa agc ctg       1296
Phe Glu Asn Val Pro Phe His Ser Ser Tyr Ala His Ser Gln Ser Leu           
            420                 425                 430                   

gac cga ctc atg aat cca ctc atc gac caa tac ttg tac tat ctc tca       1344
Asp Arg Leu Met Asn Pro Leu Ile Asp Gln Tyr Leu Tyr Tyr Leu Ser           
        435                 440                 445                       

aag act att aac ggt tct gga cag aat caa caa acg cta aaa ttc agt       1392
Lys Thr Ile Asn Gly Ser Gly Gln Asn Gln Gln Thr Leu Lys Phe Ser           
    450                 455                 460                           

gtg gcc gga ccc agc aac atg gct gtc cag gga aga aac tac ata cct       1440
Val Ala Gly Pro Ser Asn Met Ala Val Gln Gly Arg Asn Tyr Ile Pro           
465                 470                 475                 480           

gga ccc agc tac cga caa caa cgt gtc tca acc act gtg act caa aac       1488
Gly Pro Ser Tyr Arg Gln Gln Arg Val Ser Thr Thr Val Thr Gln Asn           
                485                 490                 495               

aac aac agc gaa ttt gct tgg cct gga gct tct tct tgg gct ctc aat       1536
Asn Asn Ser Glu Phe Ala Trp Pro Gly Ala Ser Ser Trp Ala Leu Asn           
            500                 505                 510                   

gga cgt aat agc ttg atg aat cct gga cct gct atg gcc agc cac aaa       1584
Gly Arg Asn Ser Leu Met Asn Pro Gly Pro Ala Met Ala Ser His Lys           
        515                 520                 525                       

gaa gga gag gac cgt ttc ttt cct ttg tct gga tct tta att ttt ggc       1632
Glu Gly Glu Asp Arg Phe Phe Pro Leu Ser Gly Ser Leu Ile Phe Gly           
    530                 535                 540                           

aaa caa gga act gga aga gac aac gtg gat gcg gac aaa gtc atg ata       1680
Lys Gln Gly Thr Gly Arg Asp Asn Val Asp Ala Asp Lys Val Met Ile           
545                 550                 555                 560           

acc aac gaa gaa gaa att aaa act acc aac cca gta gca acg gag tcc       1728
Thr Asn Glu Glu Glu Ile Lys Thr Thr Asn Pro Val Ala Thr Glu Ser           
                565                 570                 575               

tat gga caa gtg gcc aca aac cac cag agt gcc caa gca cag gcg cag       1776
Tyr Gly Gln Val Ala Thr Asn His Gln Ser Ala Gln Ala Gln Ala Gln           
            580                 585                 590                   

acc ggc tgg gtt caa aac caa gga ata ctt ccg ggt atg gtt tgg cag       1824
Thr Gly Trp Val Gln Asn Gln Gly Ile Leu Pro Gly Met Val Trp Gln           
        595                 600                 605                       

gac aga gat gtg tac ctg caa gga ccc att tgg gcc aaa att cct cac       1872
Asp Arg Asp Val Tyr Leu Gln Gly Pro Ile Trp Ala Lys Ile Pro His           
    610                 615                 620                           

acg gac ggc aac ttt cac cct tct ccg ctg atg gga ggg ttt gga atg       1920
Thr Asp Gly Asn Phe His Pro Ser Pro Leu Met Gly Gly Phe Gly Met           
625                 630                 635                 640           

aag cac ccg cct cct cag atc ctc atc aaa aac aca cct gta cct gcg       1968
Lys His Pro Pro Pro Gln Ile Leu Ile Lys Asn Thr Pro Val Pro Ala           
                645                 650                 655               

gat cct cca acg gct ttc aac aag gac aag ctg aac tct ttc atc acc       2016
Asp Pro Pro Thr Ala Phe Asn Lys Asp Lys Leu Asn Ser Phe Ile Thr           
            660                 665                 670                   

cag tat tct act ggc caa gtc agc gtg gag att gag tgg gag ctg cag       2064
Gln Tyr Ser Thr Gly Gln Val Ser Val Glu Ile Glu Trp Glu Leu Gln           
        675                 680                 685                       

aag gaa aac agc aag cgc tgg aac ccg gag atc cag tac act tcc aac       2112
Lys Glu Asn Ser Lys Arg Trp Asn Pro Glu Ile Gln Tyr Thr Ser Asn           
    690                 695                 700                           

tat tac aag tct aat aat gtt gaa ttt gct gtt aat act gaa ggt gtt       2160
Tyr Tyr Lys Ser Asn Asn Val Glu Phe Ala Val Asn Thr Glu Gly Val           
705                 710                 715                 720           

tat tct gaa ccc cgc ccc att ggc acc aga tac ctg act cgt aat ctg       2208
Tyr Ser Glu Pro Arg Pro Ile Gly Thr Arg Tyr Leu Thr Arg Asn Leu           
                725                 730                 735               

taa                                                                   2211


<210>  2
<211>  736
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  2

Met Ala Ala Asp Gly Tyr Leu Pro Asp Trp Leu Glu Asp Asn Leu Ser 
1               5                   10                  15      


Glu Gly Ile Arg Glu Trp Trp Ala Leu Lys Pro Gly Ala Pro Gln Pro 
            20                  25                  30          


Lys Ala Asn Gln Gln His Gln Asp Asn Ala Arg Gly Leu Val Leu Pro 
        35                  40                  45              


Gly Tyr Lys Tyr Leu Gly Pro Gly Asn Gly Leu Asp Lys Gly Glu Pro 
    50                  55                  60                  


Val Asn Glu Ala Asp Ala Ala Ala Leu Glu His Asp Lys Ala Tyr Asp 
65                  70                  75                  80  


Gln Gln Leu Lys Ala Gly Asp Asn Pro Tyr Leu Lys Tyr Asn His Ala 
                85                  90                  95      


Asp Ala Glu Phe Gln Glu Arg Leu Lys Glu Asp Thr Ser Phe Gly Gly 
            100                 105                 110         


Asn Leu Gly Arg Ala Val Phe Gln Ala Lys Lys Arg Leu Leu Glu Pro 
        115                 120                 125             


Leu Gly Leu Val Glu Glu Ala Ala Lys Thr Ala Pro Gly Lys Lys Arg 
    130                 135                 140                 


Pro Val Glu Gln Ser Pro Gln Glu Pro Asp Ser Ser Val Gly Ile Gly 
145                 150                 155                 160 


Lys Ser Gly Ala Gln Pro Ala Lys Lys Arg Leu Asn Phe Gly Gln Thr 
                165                 170                 175     


Gly Asp Thr Glu Ser Val Pro Asp Pro Gln Pro Ile Gly Glu Pro Pro 
            180                 185                 190         


Ala Ala Pro Ser Gly Val Gly Ser Leu Thr Met Ala Ser Gly Gly Gly 
        195                 200                 205             


Ala Pro Val Ala Asp Asn Asn Glu Gly Ala Asp Gly Val Gly Ser Ser 
    210                 215                 220                 


Ser Gly Asn Trp His Cys Asp Ser Gln Trp Leu Gly Asp Arg Val Ile 
225                 230                 235                 240 


Thr Thr Ser Thr Arg Thr Trp Ala Leu Pro Thr Tyr Asn Asn His Leu 
                245                 250                 255     


Tyr Lys Gln Ile Ser Asn Ser Thr Ser Gly Gly Ser Ser Asn Asp Asn 
            260                 265                 270         


Ala Tyr Phe Gly Tyr Ser Thr Pro Trp Gly Tyr Phe Asp Phe Asn Arg 
        275                 280                 285             


Phe His Cys His Phe Ser Pro Arg Asp Trp Gln Arg Leu Ile Asn Asn 
    290                 295                 300                 


Asn Trp Gly Phe Arg Pro Lys Arg Leu Asn Phe Lys Leu Phe Asn Ile 
305                 310                 315                 320 


Gln Val Lys Glu Val Thr Asp Asn Asn Gly Val Lys Thr Ile Ala Asn 
                325                 330                 335     


Asn Leu Thr Ser Thr Val Gln Val Phe Thr Asp Ser Asp Tyr Gln Leu 
            340                 345                 350         


Pro Tyr Val Leu Gly Ser Ala His Glu Gly Cys Leu Pro Pro Phe Pro 
        355                 360                 365             


Ala Asp Val Phe Met Ile Pro Gln Tyr Gly Tyr Leu Thr Leu Asn Asp 
    370                 375                 380                 


Gly Ser Gln Ala Val Gly Arg Ser Ser Phe Tyr Cys Leu Glu Tyr Phe 
385                 390                 395                 400 


Pro Ser Gln Met Leu Arg Thr Gly Asn Asn Phe Gln Phe Ser Tyr Glu 
                405                 410                 415     


Phe Glu Asn Val Pro Phe His Ser Ser Tyr Ala His Ser Gln Ser Leu 
            420                 425                 430         


Asp Arg Leu Met Asn Pro Leu Ile Asp Gln Tyr Leu Tyr Tyr Leu Ser 
        435                 440                 445             


Lys Thr Ile Asn Gly Ser Gly Gln Asn Gln Gln Thr Leu Lys Phe Ser 
    450                 455                 460                 


Val Ala Gly Pro Ser Asn Met Ala Val Gln Gly Arg Asn Tyr Ile Pro 
465                 470                 475                 480 


Gly Pro Ser Tyr Arg Gln Gln Arg Val Ser Thr Thr Val Thr Gln Asn 
                485                 490                 495     


Asn Asn Ser Glu Phe Ala Trp Pro Gly Ala Ser Ser Trp Ala Leu Asn 
            500                 505                 510         


Gly Arg Asn Ser Leu Met Asn Pro Gly Pro Ala Met Ala Ser His Lys 
        515                 520                 525             


Glu Gly Glu Asp Arg Phe Phe Pro Leu Ser Gly Ser Leu Ile Phe Gly 
    530                 535                 540                 


Lys Gln Gly Thr Gly Arg Asp Asn Val Asp Ala Asp Lys Val Met Ile 
545                 550                 555                 560 


Thr Asn Glu Glu Glu Ile Lys Thr Thr Asn Pro Val Ala Thr Glu Ser 
                565                 570                 575     


Tyr Gly Gln Val Ala Thr Asn His Gln Ser Ala Gln Ala Gln Ala Gln 
            580                 585                 590         


Thr Gly Trp Val Gln Asn Gln Gly Ile Leu Pro Gly Met Val Trp Gln 
        595                 600                 605             


Asp Arg Asp Val Tyr Leu Gln Gly Pro Ile Trp Ala Lys Ile Pro His 
    610                 615                 620                 


Thr Asp Gly Asn Phe His Pro Ser Pro Leu Met Gly Gly Phe Gly Met 
625                 630                 635                 640 


Lys His Pro Pro Pro Gln Ile Leu Ile Lys Asn Thr Pro Val Pro Ala 
                645                 650                 655     


Asp Pro Pro Thr Ala Phe Asn Lys Asp Lys Leu Asn Ser Phe Ile Thr 
            660                 665                 670         


Gln Tyr Ser Thr Gly Gln Val Ser Val Glu Ile Glu Trp Glu Leu Gln 
        675                 680                 685             


Lys Glu Asn Ser Lys Arg Trp Asn Pro Glu Ile Gln Tyr Thr Ser Asn 
    690                 695                 700                 


Tyr Tyr Lys Ser Asn Asn Val Glu Phe Ala Val Asn Thr Glu Gly Val 
705                 710                 715                 720 


Tyr Ser Glu Pro Arg Pro Ile Gly Thr Arg Tyr Leu Thr Arg Asn Leu 
                725                 730                 735     


<210>  3
<211>  2208
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  AAV9 VP1 capsid


<220>
<221>  CDS
<222>  (1)..(2208)
<223>  AAV9 VP1 Capsid

<400>  3
atg gct gcc gat ggt tat ctt cca gat tgg ctc gag gac aac ctt agt         48
Met Ala Ala Asp Gly Tyr Leu Pro Asp Trp Leu Glu Asp Asn Leu Ser           
1               5                   10                  15                

gaa gga att cgc gag tgg tgg gct ttg aaa cct gga gcc cct caa ccc         96
Glu Gly Ile Arg Glu Trp Trp Ala Leu Lys Pro Gly Ala Pro Gln Pro           
            20                  25                  30                    

aag gca aat caa caa cat caa gac aac gct cga ggt ctt gtg ctt ccg        144
Lys Ala Asn Gln Gln His Gln Asp Asn Ala Arg Gly Leu Val Leu Pro           
        35                  40                  45                        

ggt tac aaa tac ctt gga ccc ggc aac gga ctc gac aag ggg gag ccg        192
Gly Tyr Lys Tyr Leu Gly Pro Gly Asn Gly Leu Asp Lys Gly Glu Pro           
    50                  55                  60                            

gtc aac gca gca gac gcg gcg gcc ctc gag cac gac aag gcc tac gac        240
Val Asn Ala Ala Asp Ala Ala Ala Leu Glu His Asp Lys Ala Tyr Asp           
65                  70                  75                  80            

cag cag ctc aag gcc gga gac aac ccg tac ctc aag tac aac cac gcc        288
Gln Gln Leu Lys Ala Gly Asp Asn Pro Tyr Leu Lys Tyr Asn His Ala           
                85                  90                  95                

gac gcc gag ttc cag gag cgg ctc aaa gaa gat acg tct ttt ggg ggc        336
Asp Ala Glu Phe Gln Glu Arg Leu Lys Glu Asp Thr Ser Phe Gly Gly           
            100                 105                 110                   

aac ctc ggg cga gca gtc ttc cag gcc aaa aag agg ctt ctt gaa cct        384
Asn Leu Gly Arg Ala Val Phe Gln Ala Lys Lys Arg Leu Leu Glu Pro           
        115                 120                 125                       

ctt ggt ctg gtt gag gaa gcg gct aag acg gct cct gga aag aag agg        432
Leu Gly Leu Val Glu Glu Ala Ala Lys Thr Ala Pro Gly Lys Lys Arg           
    130                 135                 140                           

cct gta gag cag tct cct cag gaa ccg gac tcc tcc gcg ggt att ggc        480
Pro Val Glu Gln Ser Pro Gln Glu Pro Asp Ser Ser Ala Gly Ile Gly           
145                 150                 155                 160           

aaa tcg ggt gca cag ccc gct aaa aag aga ctc aat ttc ggt cag act        528
Lys Ser Gly Ala Gln Pro Ala Lys Lys Arg Leu Asn Phe Gly Gln Thr           
                165                 170                 175               

ggc gac aca gag tca gtc cca gac cct caa cca atc gga gaa cct ccc        576
Gly Asp Thr Glu Ser Val Pro Asp Pro Gln Pro Ile Gly Glu Pro Pro           
            180                 185                 190                   

gca gcc ccc tca ggt gtg gga tct ctt aca atg gct tca ggt ggt ggc        624
Ala Ala Pro Ser Gly Val Gly Ser Leu Thr Met Ala Ser Gly Gly Gly           
        195                 200                 205                       

gca cca gtg gca gac aat aac gaa ggt gcc gat gga gtg ggt agt tcc        672
Ala Pro Val Ala Asp Asn Asn Glu Gly Ala Asp Gly Val Gly Ser Ser           
    210                 215                 220                           

tcg gga aat tgg cat tgc gat tcc caa tgg ctg ggg gac aga gtc atc        720
Ser Gly Asn Trp His Cys Asp Ser Gln Trp Leu Gly Asp Arg Val Ile           
225                 230                 235                 240           

acc acc agc acc cga acc tgg gcc ctg ccc acc tac aac aat cac ctc        768
Thr Thr Ser Thr Arg Thr Trp Ala Leu Pro Thr Tyr Asn Asn His Leu           
                245                 250                 255               

tac aag caa atc tcc aac agc aca tct gga gga tct tca aat gac aac        816
Tyr Lys Gln Ile Ser Asn Ser Thr Ser Gly Gly Ser Ser Asn Asp Asn           
            260                 265                 270                   

gcc tac ttc ggc tac agc acc ccc tgg ggg tat ttt gac ttc aac aga        864
Ala Tyr Phe Gly Tyr Ser Thr Pro Trp Gly Tyr Phe Asp Phe Asn Arg           
        275                 280                 285                       

ttc cac tgc cac ttc tca cca cgt gac tgg cag cga ctc atc aac aac        912
Phe His Cys His Phe Ser Pro Arg Asp Trp Gln Arg Leu Ile Asn Asn           
    290                 295                 300                           

aac tgg gga ttc cgg cct aag cga ctc aac ttc aag ctc ttc aac att        960
Asn Trp Gly Phe Arg Pro Lys Arg Leu Asn Phe Lys Leu Phe Asn Ile           
305                 310                 315                 320           

cag gtc aaa gag gtt acg gac aac aat gga gtc aag acc atc gcc aat       1008
Gln Val Lys Glu Val Thr Asp Asn Asn Gly Val Lys Thr Ile Ala Asn           
                325                 330                 335               

aac ctt acc agc acg gtc cag gtc ttc acg gac tca gac tat cag ctc       1056
Asn Leu Thr Ser Thr Val Gln Val Phe Thr Asp Ser Asp Tyr Gln Leu           
            340                 345                 350                   

ccg tac gtg ctc ggg tcg gct cac gag ggc tgc ctc ccg ccg ttc cca       1104
Pro Tyr Val Leu Gly Ser Ala His Glu Gly Cys Leu Pro Pro Phe Pro           
        355                 360                 365                       

gcg gac gtt ttc atg att cct cag tac ggg tat ctg acg ctt aat gat       1152
Ala Asp Val Phe Met Ile Pro Gln Tyr Gly Tyr Leu Thr Leu Asn Asp           
    370                 375                 380                           

gga agc cag gcc gtg ggt cgt tcg tcc ttt tac tgc ctg gaa tat ttc       1200
Gly Ser Gln Ala Val Gly Arg Ser Ser Phe Tyr Cys Leu Glu Tyr Phe           
385                 390                 395                 400           

ccg tcg caa atg cta aga acg ggt aac aac ttc cag ttc agc tac gag       1248
Pro Ser Gln Met Leu Arg Thr Gly Asn Asn Phe Gln Phe Ser Tyr Glu           
                405                 410                 415               

ttt gag aac gta cct ttc cat agc agc tac gct cac agc caa agc ctg       1296
Phe Glu Asn Val Pro Phe His Ser Ser Tyr Ala His Ser Gln Ser Leu           
            420                 425                 430                   

gac cga cta atg aat cca ctc atc gac caa tac ttg tac tat ctc tca       1344
Asp Arg Leu Met Asn Pro Leu Ile Asp Gln Tyr Leu Tyr Tyr Leu Ser           
        435                 440                 445                       

aag act att aac ggt tct gga cag aat caa caa acg cta aaa ttc agt       1392
Lys Thr Ile Asn Gly Ser Gly Gln Asn Gln Gln Thr Leu Lys Phe Ser           
    450                 455                 460                           

gtg gcc gga ccc agc aac atg gct gtc cag gga aga aac tac ata cct       1440
Val Ala Gly Pro Ser Asn Met Ala Val Gln Gly Arg Asn Tyr Ile Pro           
465                 470                 475                 480           

gga ccc agc tac cga caa caa cgt gtc tca acc act gtg act caa aac       1488
Gly Pro Ser Tyr Arg Gln Gln Arg Val Ser Thr Thr Val Thr Gln Asn           
                485                 490                 495               

aac aac agc gaa ttt gct tgg cct gga gct tct tct tgg gct ctc aat       1536
Asn Asn Ser Glu Phe Ala Trp Pro Gly Ala Ser Ser Trp Ala Leu Asn           
            500                 505                 510                   

gga cgt aat agc ttg atg aat cct gga cct gct atg gcc agc cac aaa       1584
Gly Arg Asn Ser Leu Met Asn Pro Gly Pro Ala Met Ala Ser His Lys           
        515                 520                 525                       

gaa gga gag gac cgt ttc ttt cct ttg tct gga tct tta att ttt ggc       1632
Glu Gly Glu Asp Arg Phe Phe Pro Leu Ser Gly Ser Leu Ile Phe Gly           
    530                 535                 540                           

aaa caa gga act gga aga gac aac gtg gat gcg gac aaa gtc atg ata       1680
Lys Gln Gly Thr Gly Arg Asp Asn Val Asp Ala Asp Lys Val Met Ile           
545                 550                 555                 560           

acc aac gaa gaa gaa att aaa act act aac ccg gta gca acg gag tcc       1728
Thr Asn Glu Glu Glu Ile Lys Thr Thr Asn Pro Val Ala Thr Glu Ser           
                565                 570                 575               

tat gga caa gtg gcc aca aac cac cag agt gcc caa gca cag gcg cag       1776
Tyr Gly Gln Val Ala Thr Asn His Gln Ser Ala Gln Ala Gln Ala Gln           
            580                 585                 590                   

acc ggc tgg gtt caa aac caa gga ata ctt ccg ggt atg gtt tgg cag       1824
Thr Gly Trp Val Gln Asn Gln Gly Ile Leu Pro Gly Met Val Trp Gln           
        595                 600                 605                       

gac aga gat gtg tac ctg caa gga ccc att tgg gcc aaa att cct cac       1872
Asp Arg Asp Val Tyr Leu Gln Gly Pro Ile Trp Ala Lys Ile Pro His           
    610                 615                 620                           

acg gac ggc aac ttt cac cct tct ccg ctg atg gga ggg ttt gga atg       1920
Thr Asp Gly Asn Phe His Pro Ser Pro Leu Met Gly Gly Phe Gly Met           
625                 630                 635                 640           

aag cac ccg cct cct cag atc ctc atc aaa aac aca cct gta cct gcg       1968
Lys His Pro Pro Pro Gln Ile Leu Ile Lys Asn Thr Pro Val Pro Ala           
                645                 650                 655               

gat cct cca acg gcc ttc aac aag gac aag ctg aac tct ttc atc acc       2016
Asp Pro Pro Thr Ala Phe Asn Lys Asp Lys Leu Asn Ser Phe Ile Thr           
            660                 665                 670                   

cag tat tct act ggc caa gtc agc gtg gag atc gag tgg gag ctg cag       2064
Gln Tyr Ser Thr Gly Gln Val Ser Val Glu Ile Glu Trp Glu Leu Gln           
        675                 680                 685                       

aag gaa aac agc aag cgc tgg aac ccg gag atc cag tac act tcc aac       2112
Lys Glu Asn Ser Lys Arg Trp Asn Pro Glu Ile Gln Tyr Thr Ser Asn           
    690                 695                 700                           

tat tac aag tct aat aat gtt gaa ttt gct gtt aat act gaa ggt gta       2160
Tyr Tyr Lys Ser Asn Asn Val Glu Phe Ala Val Asn Thr Glu Gly Val           
705                 710                 715                 720           

tat agt gaa ccc cgc ccc att ggc acc aga tac ctg act cgt aat ctg       2208
Tyr Ser Glu Pro Arg Pro Ile Gly Thr Arg Tyr Leu Thr Arg Asn Leu           
                725                 730                 735               


<210>  4
<211>  736
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  4

Met Ala Ala Asp Gly Tyr Leu Pro Asp Trp Leu Glu Asp Asn Leu Ser 
1               5                   10                  15      


Glu Gly Ile Arg Glu Trp Trp Ala Leu Lys Pro Gly Ala Pro Gln Pro 
            20                  25                  30          


Lys Ala Asn Gln Gln His Gln Asp Asn Ala Arg Gly Leu Val Leu Pro 
        35                  40                  45              


Gly Tyr Lys Tyr Leu Gly Pro Gly Asn Gly Leu Asp Lys Gly Glu Pro 
    50                  55                  60                  


Val Asn Ala Ala Asp Ala Ala Ala Leu Glu His Asp Lys Ala Tyr Asp 
65                  70                  75                  80  


Gln Gln Leu Lys Ala Gly Asp Asn Pro Tyr Leu Lys Tyr Asn His Ala 
                85                  90                  95      


Asp Ala Glu Phe Gln Glu Arg Leu Lys Glu Asp Thr Ser Phe Gly Gly 
            100                 105                 110         


Asn Leu Gly Arg Ala Val Phe Gln Ala Lys Lys Arg Leu Leu Glu Pro 
        115                 120                 125             


Leu Gly Leu Val Glu Glu Ala Ala Lys Thr Ala Pro Gly Lys Lys Arg 
    130                 135                 140                 


Pro Val Glu Gln Ser Pro Gln Glu Pro Asp Ser Ser Ala Gly Ile Gly 
145                 150                 155                 160 


Lys Ser Gly Ala Gln Pro Ala Lys Lys Arg Leu Asn Phe Gly Gln Thr 
                165                 170                 175     


Gly Asp Thr Glu Ser Val Pro Asp Pro Gln Pro Ile Gly Glu Pro Pro 
            180                 185                 190         


Ala Ala Pro Ser Gly Val Gly Ser Leu Thr Met Ala Ser Gly Gly Gly 
        195                 200                 205             


Ala Pro Val Ala Asp Asn Asn Glu Gly Ala Asp Gly Val Gly Ser Ser 
    210                 215                 220                 


Ser Gly Asn Trp His Cys Asp Ser Gln Trp Leu Gly Asp Arg Val Ile 
225                 230                 235                 240 


Thr Thr Ser Thr Arg Thr Trp Ala Leu Pro Thr Tyr Asn Asn His Leu 
                245                 250                 255     


Tyr Lys Gln Ile Ser Asn Ser Thr Ser Gly Gly Ser Ser Asn Asp Asn 
            260                 265                 270         


Ala Tyr Phe Gly Tyr Ser Thr Pro Trp Gly Tyr Phe Asp Phe Asn Arg 
        275                 280                 285             


Phe His Cys His Phe Ser Pro Arg Asp Trp Gln Arg Leu Ile Asn Asn 
    290                 295                 300                 


Asn Trp Gly Phe Arg Pro Lys Arg Leu Asn Phe Lys Leu Phe Asn Ile 
305                 310                 315                 320 


Gln Val Lys Glu Val Thr Asp Asn Asn Gly Val Lys Thr Ile Ala Asn 
                325                 330                 335     


Asn Leu Thr Ser Thr Val Gln Val Phe Thr Asp Ser Asp Tyr Gln Leu 
            340                 345                 350         


Pro Tyr Val Leu Gly Ser Ala His Glu Gly Cys Leu Pro Pro Phe Pro 
        355                 360                 365             


Ala Asp Val Phe Met Ile Pro Gln Tyr Gly Tyr Leu Thr Leu Asn Asp 
    370                 375                 380                 


Gly Ser Gln Ala Val Gly Arg Ser Ser Phe Tyr Cys Leu Glu Tyr Phe 
385                 390                 395                 400 


Pro Ser Gln Met Leu Arg Thr Gly Asn Asn Phe Gln Phe Ser Tyr Glu 
                405                 410                 415     


Phe Glu Asn Val Pro Phe His Ser Ser Tyr Ala His Ser Gln Ser Leu 
            420                 425                 430         


Asp Arg Leu Met Asn Pro Leu Ile Asp Gln Tyr Leu Tyr Tyr Leu Ser 
        435                 440                 445             


Lys Thr Ile Asn Gly Ser Gly Gln Asn Gln Gln Thr Leu Lys Phe Ser 
    450                 455                 460                 


Val Ala Gly Pro Ser Asn Met Ala Val Gln Gly Arg Asn Tyr Ile Pro 
465                 470                 475                 480 


Gly Pro Ser Tyr Arg Gln Gln Arg Val Ser Thr Thr Val Thr Gln Asn 
                485                 490                 495     


Asn Asn Ser Glu Phe Ala Trp Pro Gly Ala Ser Ser Trp Ala Leu Asn 
            500                 505                 510         


Gly Arg Asn Ser Leu Met Asn Pro Gly Pro Ala Met Ala Ser His Lys 
        515                 520                 525             


Glu Gly Glu Asp Arg Phe Phe Pro Leu Ser Gly Ser Leu Ile Phe Gly 
    530                 535                 540                 


Lys Gln Gly Thr Gly Arg Asp Asn Val Asp Ala Asp Lys Val Met Ile 
545                 550                 555                 560 


Thr Asn Glu Glu Glu Ile Lys Thr Thr Asn Pro Val Ala Thr Glu Ser 
                565                 570                 575     


Tyr Gly Gln Val Ala Thr Asn His Gln Ser Ala Gln Ala Gln Ala Gln 
            580                 585                 590         


Thr Gly Trp Val Gln Asn Gln Gly Ile Leu Pro Gly Met Val Trp Gln 
        595                 600                 605             


Asp Arg Asp Val Tyr Leu Gln Gly Pro Ile Trp Ala Lys Ile Pro His 
    610                 615                 620                 


Thr Asp Gly Asn Phe His Pro Ser Pro Leu Met Gly Gly Phe Gly Met 
625                 630                 635                 640 


Lys His Pro Pro Pro Gln Ile Leu Ile Lys Asn Thr Pro Val Pro Ala 
                645                 650                 655     


Asp Pro Pro Thr Ala Phe Asn Lys Asp Lys Leu Asn Ser Phe Ile Thr 
            660                 665                 670         


Gln Tyr Ser Thr Gly Gln Val Ser Val Glu Ile Glu Trp Glu Leu Gln 
        675                 680                 685             


Lys Glu Asn Ser Lys Arg Trp Asn Pro Glu Ile Gln Tyr Thr Ser Asn 
    690                 695                 700                 


Tyr Tyr Lys Ser Asn Asn Val Glu Phe Ala Val Asn Thr Glu Gly Val 
705                 710                 715                 720 


Tyr Ser Glu Pro Arg Pro Ile Gly Thr Arg Tyr Leu Thr Arg Asn Leu 
                725                 730                 735     


<210>  5
<211>  2058
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Human GALC coding sequence


<220>
<221>  sig_peptide
<222>  (1)..(126)

<220>
<221>  CDS
<222>  (1)..(2058)

<400>  5
atg gct gag tgg cta ctc tcg gct tcc tgg caa cgc cga gcg aaa gct         48
Met Ala Glu Trp Leu Leu Ser Ala Ser Trp Gln Arg Arg Ala Lys Ala           
1               5                   10                  15                

atg act gcg gcc gcg ggt tcg gcg ggc cgc gcc gcg gtg ccc ttg ctg         96
Met Thr Ala Ala Ala Gly Ser Ala Gly Arg Ala Ala Val Pro Leu Leu           
            20                  25                  30                    

ctg tgt gcg ctg ctg gcg ccc ggc ggc gcg tac gtg ctc gac gac tcc        144
Leu Cys Ala Leu Leu Ala Pro Gly Gly Ala Tyr Val Leu Asp Asp Ser           
        35                  40                  45                        

gac ggg ctg ggc cgg gag ttc gac ggc atc ggc gcg gtc agc ggc ggc        192
Asp Gly Leu Gly Arg Glu Phe Asp Gly Ile Gly Ala Val Ser Gly Gly           
    50                  55                  60                            

ggg gca acc tcc cga ctt cta gta aat tac cca gag ccc tat cgt tct        240
Gly Ala Thr Ser Arg Leu Leu Val Asn Tyr Pro Glu Pro Tyr Arg Ser           
65                  70                  75                  80            

cag ata ttg gat tat ctc ttt aag ccg aat ttt ggt gcc tct ttg cat        288
Gln Ile Leu Asp Tyr Leu Phe Lys Pro Asn Phe Gly Ala Ser Leu His           
                85                  90                  95                

att tta aaa gtg gaa ata ggt ggt gat ggg cag aca aca gac ggc act        336
Ile Leu Lys Val Glu Ile Gly Gly Asp Gly Gln Thr Thr Asp Gly Thr           
            100                 105                 110                   

gag ccc tcc cac atg cat tat gca cta gat gag aat tat ttc cga gga        384
Glu Pro Ser His Met His Tyr Ala Leu Asp Glu Asn Tyr Phe Arg Gly           
        115                 120                 125                       

tac gag tgg tgg ttg atg aaa gaa gct aag aag agg aat ccc aat att        432
Tyr Glu Trp Trp Leu Met Lys Glu Ala Lys Lys Arg Asn Pro Asn Ile           
    130                 135                 140                           

aca ctc att ggg ttg cca tgg tca ttc cct gga tgg ctg gga aaa ggt        480
Thr Leu Ile Gly Leu Pro Trp Ser Phe Pro Gly Trp Leu Gly Lys Gly           
145                 150                 155                 160           

ttc gac tgg cct tat gtc aat ctt cag ctg act gcc tat tat gtc gtg        528
Phe Asp Trp Pro Tyr Val Asn Leu Gln Leu Thr Ala Tyr Tyr Val Val           
                165                 170                 175               

acc tgg att gtg ggc gcc aag cgt tac cat gat ttg gac att gat tat        576
Thr Trp Ile Val Gly Ala Lys Arg Tyr His Asp Leu Asp Ile Asp Tyr           
            180                 185                 190                   

att gga att tgg aat gag agg tca tat aat gcc aat tat att aag ata        624
Ile Gly Ile Trp Asn Glu Arg Ser Tyr Asn Ala Asn Tyr Ile Lys Ile           
        195                 200                 205                       

tta aga aaa atg ctg aat tat caa ggt ctc cag cga gtg aaa atc ata        672
Leu Arg Lys Met Leu Asn Tyr Gln Gly Leu Gln Arg Val Lys Ile Ile           
    210                 215                 220                           

gca agt gat aat ctc tgg gag tcc atc tct gca tcc atg ctc ctt gat        720
Ala Ser Asp Asn Leu Trp Glu Ser Ile Ser Ala Ser Met Leu Leu Asp           
225                 230                 235                 240           

gcc gaa ctc ttc aag gtg gtt gat gtt ata ggg gct cat tat cct gga        768
Ala Glu Leu Phe Lys Val Val Asp Val Ile Gly Ala His Tyr Pro Gly           
                245                 250                 255               

acc cat tca gca aaa gat gca aag ttg act ggg aag aag ctt tgg tct        816
Thr His Ser Ala Lys Asp Ala Lys Leu Thr Gly Lys Lys Leu Trp Ser           
            260                 265                 270                   

tct gaa gac ttt agc act tta aat agt gac atg ggt gca ggc tgc tgg        864
Ser Glu Asp Phe Ser Thr Leu Asn Ser Asp Met Gly Ala Gly Cys Trp           
        275                 280                 285                       

ggt cgc att tta aat cag aat tat atc aat ggc tat atg act tcc aca        912
Gly Arg Ile Leu Asn Gln Asn Tyr Ile Asn Gly Tyr Met Thr Ser Thr           
    290                 295                 300                           

atc gca tgg aat tta gtg gct agt tac tat gaa cag ttg cct tat ggg        960
Ile Ala Trp Asn Leu Val Ala Ser Tyr Tyr Glu Gln Leu Pro Tyr Gly           
305                 310                 315                 320           

aga tgc ggg ttg atg acg gcc cag gag cca tgg agt ggg cac tac gtg       1008
Arg Cys Gly Leu Met Thr Ala Gln Glu Pro Trp Ser Gly His Tyr Val           
                325                 330                 335               

gta gaa tct cct gtc tgg gta tca gct cat acc act cag ttt act caa       1056
Val Glu Ser Pro Val Trp Val Ser Ala His Thr Thr Gln Phe Thr Gln           
            340                 345                 350                   

cct ggc tgg tat tac ctg aag aca gtt ggc cat tta gag aaa gga gga       1104
Pro Gly Trp Tyr Tyr Leu Lys Thr Val Gly His Leu Glu Lys Gly Gly           
        355                 360                 365                       

agc tac gta gct ctg act gat ggc tta ggg aac ctc acc atc atc att       1152
Ser Tyr Val Ala Leu Thr Asp Gly Leu Gly Asn Leu Thr Ile Ile Ile           
    370                 375                 380                           

gaa acc atg agt cat aaa cat tct aag tgc ata cgg cca ttt ctt cct       1200
Glu Thr Met Ser His Lys His Ser Lys Cys Ile Arg Pro Phe Leu Pro           
385                 390                 395                 400           

tat ttc aat gtg tca caa caa ttt gcc acc ttt gtt ctt aag gga tct       1248
Tyr Phe Asn Val Ser Gln Gln Phe Ala Thr Phe Val Leu Lys Gly Ser           
                405                 410                 415               

ttt agt gaa ata cca gag cta cag gta tgg tat acc aaa ctt gga aaa       1296
Phe Ser Glu Ile Pro Glu Leu Gln Val Trp Tyr Thr Lys Leu Gly Lys           
            420                 425                 430                   

aca tcc gaa aga ttt ctt ttt aag cag ctg gat tct cta tgg ctc ctt       1344
Thr Ser Glu Arg Phe Leu Phe Lys Gln Leu Asp Ser Leu Trp Leu Leu           
        435                 440                 445                       

gac agc gat ggc agt ttc aca ctg agc ctg cat gaa gat gag ctg ttc       1392
Asp Ser Asp Gly Ser Phe Thr Leu Ser Leu His Glu Asp Glu Leu Phe           
    450                 455                 460                           

aca ctc acc act ctc acc act ggt cgc aaa ggc agc tac ccg ctt cct       1440
Thr Leu Thr Thr Leu Thr Thr Gly Arg Lys Gly Ser Tyr Pro Leu Pro           
465                 470                 475                 480           

cca aaa tcc cag ccc ttc cca agt acc tat aag gat gat ttc aat gtt       1488
Pro Lys Ser Gln Pro Phe Pro Ser Thr Tyr Lys Asp Asp Phe Asn Val           
                485                 490                 495               

gat tac cca ttt ttt agt gaa gct cca aac ttt gct gat caa act ggt       1536
Asp Tyr Pro Phe Phe Ser Glu Ala Pro Asn Phe Ala Asp Gln Thr Gly           
            500                 505                 510                   

gta ttt gaa tat ttt aca aat att gaa gac cct ggc gag cat cac ttc       1584
Val Phe Glu Tyr Phe Thr Asn Ile Glu Asp Pro Gly Glu His His Phe           
        515                 520                 525                       

acg cta cgc caa gtt ctc aac cag aga ccc att acg tgg gct gcc gat       1632
Thr Leu Arg Gln Val Leu Asn Gln Arg Pro Ile Thr Trp Ala Ala Asp           
    530                 535                 540                           

gca tcc aac aca atc agt att ata gga gac tac aac tgg acc aat ctg       1680
Ala Ser Asn Thr Ile Ser Ile Ile Gly Asp Tyr Asn Trp Thr Asn Leu           
545                 550                 555                 560           

act ata aag tgt gat gta tac ata gag acc cct gac aca gga ggt gtg       1728
Thr Ile Lys Cys Asp Val Tyr Ile Glu Thr Pro Asp Thr Gly Gly Val           
                565                 570                 575               

ttc att gca gga aga gta aat aaa ggt ggt att ttg att aga agt gcc       1776
Phe Ile Ala Gly Arg Val Asn Lys Gly Gly Ile Leu Ile Arg Ser Ala           
            580                 585                 590                   

aga gga att ttc ttc tgg att ttt gca aat gga tct tac agg gtt aca       1824
Arg Gly Ile Phe Phe Trp Ile Phe Ala Asn Gly Ser Tyr Arg Val Thr           
        595                 600                 605                       

ggt gat tta gct gga tgg att ata tat gct tta gga cgt gtt gaa gtt       1872
Gly Asp Leu Ala Gly Trp Ile Ile Tyr Ala Leu Gly Arg Val Glu Val           
    610                 615                 620                           

aca gca aaa aaa tgg tat aca ctc acg tta act att aag ggt cat ttc       1920
Thr Ala Lys Lys Trp Tyr Thr Leu Thr Leu Thr Ile Lys Gly His Phe           
625                 630                 635                 640           

acc tct ggc atg ctg aat gac aag tct ctg tgg aca gac atc cct gtg       1968
Thr Ser Gly Met Leu Asn Asp Lys Ser Leu Trp Thr Asp Ile Pro Val           
                645                 650                 655               

aat ttt cca aag aat ggc tgg gct gca att gga act cac tcc ttt gaa       2016
Asn Phe Pro Lys Asn Gly Trp Ala Ala Ile Gly Thr His Ser Phe Glu           
            660                 665                 670                   

ttt gca cag ttt gac aac ttt ctt gtg gaa gcc aca cgc taa               2058
Phe Ala Gln Phe Asp Asn Phe Leu Val Glu Ala Thr Arg                       
        675                 680                 685                       


<210>  6
<211>  685
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Synthetic Construct

<400>  6

Met Ala Glu Trp Leu Leu Ser Ala Ser Trp Gln Arg Arg Ala Lys Ala 
1               5                   10                  15      


Met Thr Ala Ala Ala Gly Ser Ala Gly Arg Ala Ala Val Pro Leu Leu 
            20                  25                  30          


Leu Cys Ala Leu Leu Ala Pro Gly Gly Ala Tyr Val Leu Asp Asp Ser 
        35                  40                  45              


Asp Gly Leu Gly Arg Glu Phe Asp Gly Ile Gly Ala Val Ser Gly Gly 
    50                  55                  60                  


Gly Ala Thr Ser Arg Leu Leu Val Asn Tyr Pro Glu Pro Tyr Arg Ser 
65                  70                  75                  80  


Gln Ile Leu Asp Tyr Leu Phe Lys Pro Asn Phe Gly Ala Ser Leu His 
                85                  90                  95      


Ile Leu Lys Val Glu Ile Gly Gly Asp Gly Gln Thr Thr Asp Gly Thr 
            100                 105                 110         


Glu Pro Ser His Met His Tyr Ala Leu Asp Glu Asn Tyr Phe Arg Gly 
        115                 120                 125             


Tyr Glu Trp Trp Leu Met Lys Glu Ala Lys Lys Arg Asn Pro Asn Ile 
    130                 135                 140                 


Thr Leu Ile Gly Leu Pro Trp Ser Phe Pro Gly Trp Leu Gly Lys Gly 
145                 150                 155                 160 


Phe Asp Trp Pro Tyr Val Asn Leu Gln Leu Thr Ala Tyr Tyr Val Val 
                165                 170                 175     


Thr Trp Ile Val Gly Ala Lys Arg Tyr His Asp Leu Asp Ile Asp Tyr 
            180                 185                 190         


Ile Gly Ile Trp Asn Glu Arg Ser Tyr Asn Ala Asn Tyr Ile Lys Ile 
        195                 200                 205             


Leu Arg Lys Met Leu Asn Tyr Gln Gly Leu Gln Arg Val Lys Ile Ile 
    210                 215                 220                 


Ala Ser Asp Asn Leu Trp Glu Ser Ile Ser Ala Ser Met Leu Leu Asp 
225                 230                 235                 240 


Ala Glu Leu Phe Lys Val Val Asp Val Ile Gly Ala His Tyr Pro Gly 
                245                 250                 255     


Thr His Ser Ala Lys Asp Ala Lys Leu Thr Gly Lys Lys Leu Trp Ser 
            260                 265                 270         


Ser Glu Asp Phe Ser Thr Leu Asn Ser Asp Met Gly Ala Gly Cys Trp 
        275                 280                 285             


Gly Arg Ile Leu Asn Gln Asn Tyr Ile Asn Gly Tyr Met Thr Ser Thr 
    290                 295                 300                 


Ile Ala Trp Asn Leu Val Ala Ser Tyr Tyr Glu Gln Leu Pro Tyr Gly 
305                 310                 315                 320 


Arg Cys Gly Leu Met Thr Ala Gln Glu Pro Trp Ser Gly His Tyr Val 
                325                 330                 335     


Val Glu Ser Pro Val Trp Val Ser Ala His Thr Thr Gln Phe Thr Gln 
            340                 345                 350         


Pro Gly Trp Tyr Tyr Leu Lys Thr Val Gly His Leu Glu Lys Gly Gly 
        355                 360                 365             


Ser Tyr Val Ala Leu Thr Asp Gly Leu Gly Asn Leu Thr Ile Ile Ile 
    370                 375                 380                 


Glu Thr Met Ser His Lys His Ser Lys Cys Ile Arg Pro Phe Leu Pro 
385                 390                 395                 400 


Tyr Phe Asn Val Ser Gln Gln Phe Ala Thr Phe Val Leu Lys Gly Ser 
                405                 410                 415     


Phe Ser Glu Ile Pro Glu Leu Gln Val Trp Tyr Thr Lys Leu Gly Lys 
            420                 425                 430         


Thr Ser Glu Arg Phe Leu Phe Lys Gln Leu Asp Ser Leu Trp Leu Leu 
        435                 440                 445             


Asp Ser Asp Gly Ser Phe Thr Leu Ser Leu His Glu Asp Glu Leu Phe 
    450                 455                 460                 


Thr Leu Thr Thr Leu Thr Thr Gly Arg Lys Gly Ser Tyr Pro Leu Pro 
465                 470                 475                 480 


Pro Lys Ser Gln Pro Phe Pro Ser Thr Tyr Lys Asp Asp Phe Asn Val 
                485                 490                 495     


Asp Tyr Pro Phe Phe Ser Glu Ala Pro Asn Phe Ala Asp Gln Thr Gly 
            500                 505                 510         


Val Phe Glu Tyr Phe Thr Asn Ile Glu Asp Pro Gly Glu His His Phe 
        515                 520                 525             


Thr Leu Arg Gln Val Leu Asn Gln Arg Pro Ile Thr Trp Ala Ala Asp 
    530                 535                 540                 


Ala Ser Asn Thr Ile Ser Ile Ile Gly Asp Tyr Asn Trp Thr Asn Leu 
545                 550                 555                 560 


Thr Ile Lys Cys Asp Val Tyr Ile Glu Thr Pro Asp Thr Gly Gly Val 
                565                 570                 575     


Phe Ile Ala Gly Arg Val Asn Lys Gly Gly Ile Leu Ile Arg Ser Ala 
            580                 585                 590         


Arg Gly Ile Phe Phe Trp Ile Phe Ala Asn Gly Ser Tyr Arg Val Thr 
        595                 600                 605             


Gly Asp Leu Ala Gly Trp Ile Ile Tyr Ala Leu Gly Arg Val Glu Val 
    610                 615                 620                 


Thr Ala Lys Lys Trp Tyr Thr Leu Thr Leu Thr Ile Lys Gly His Phe 
625                 630                 635                 640 


Thr Ser Gly Met Leu Asn Asp Lys Ser Leu Trp Thr Asp Ile Pro Val 
                645                 650                 655     


Asn Phe Pro Lys Asn Gly Trp Ala Ala Ile Gly Thr His Ser Phe Glu 
            660                 665                 670         


Phe Ala Gln Phe Asp Asn Phe Leu Val Glu Ala Thr Arg 
        675                 680                 685 


<210>  7
<211>  2007
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Engineered canine GALC


<220>
<221>  CDS
<222>  (1)..(2007)

<400>  7
atg aca gcc gct gct gga tct gct gga cac gct gct gtt cct ctg ctg         48
Met Thr Ala Ala Ala Gly Ser Ala Gly His Ala Ala Val Pro Leu Leu           
1               5                   10                  15                

ctg tgt gca ctg ctg gtg cct ggc gga gct tac gtg ctg gat gat tct         96
Leu Cys Ala Leu Leu Val Pro Gly Gly Ala Tyr Val Leu Asp Asp Ser           
            20                  25                  30                    

gat gga cta ggc cgc gag ttc gac ggc gtg gga gct gtt tct ggt ggc        144
Asp Gly Leu Gly Arg Glu Phe Asp Gly Val Gly Ala Val Ser Gly Gly           
        35                  40                  45                        

gga gcc aca tct agg ctg ctg gtc aat tac ccc gag cct tac agg tcc        192
Gly Ala Thr Ser Arg Leu Leu Val Asn Tyr Pro Glu Pro Tyr Arg Ser           
    50                  55                  60                            

cag atc ctg gac tac ctg ttc aag ccc aac ttc ggc gcc agc ctg cac        240
Gln Ile Leu Asp Tyr Leu Phe Lys Pro Asn Phe Gly Ala Ser Leu His           
65                  70                  75                  80            

atc ctg aag gtg gaa att ggc ggc gac ggc cag acc acc gac gga aca        288
Ile Leu Lys Val Glu Ile Gly Gly Asp Gly Gln Thr Thr Asp Gly Thr           
                85                  90                  95                

gaa cct agc cac atg cac tac gcc ctg gac gag aac ttc ttc agg ggc        336
Glu Pro Ser His Met His Tyr Ala Leu Asp Glu Asn Phe Phe Arg Gly           
            100                 105                 110                   

tac gag tgg tgg ctg atg aag gaa gcc aag aag agg aac ccc aac atc        384
Tyr Glu Trp Trp Leu Met Lys Glu Ala Lys Lys Arg Asn Pro Asn Ile           
        115                 120                 125                       

atc ctg atg ggc ctg cct tgg tct ttc cct ggc tgg atc ggc aag ggc        432
Ile Leu Met Gly Leu Pro Trp Ser Phe Pro Gly Trp Ile Gly Lys Gly           
    130                 135                 140                           

ttc aac tgg cct tac gtg aac ctg cag ctg acc gcc tac tac atc atg        480
Phe Asn Trp Pro Tyr Val Asn Leu Gln Leu Thr Ala Tyr Tyr Ile Met           
145                 150                 155                 160           

acc tgg atc gtg ggc gcc aag cac tac cac gac ctg gac atc gac tac        528
Thr Trp Ile Val Gly Ala Lys His Tyr His Asp Leu Asp Ile Asp Tyr           
                165                 170                 175               

atc ggc atc tgg aac gag cgg agc ttc gac atc aat tac atc aag gtg        576
Ile Gly Ile Trp Asn Glu Arg Ser Phe Asp Ile Asn Tyr Ile Lys Val           
            180                 185                 190                   

ctg cgg cgg atg ctg aac tac cag ggc ctc gac aga gtg aag atc att        624
Leu Arg Arg Met Leu Asn Tyr Gln Gly Leu Asp Arg Val Lys Ile Ile           
        195                 200                 205                       

gcc agc gac aac ctg tgg gag ccc atc agc gct tct atg ctg ctg gac        672
Ala Ser Asp Asn Leu Trp Glu Pro Ile Ser Ala Ser Met Leu Leu Asp           
    210                 215                 220                           

tcc gag ctg ctg aaa gtg atc gac gtg atc ggc gct cac tac ccc gga        720
Ser Glu Leu Leu Lys Val Ile Asp Val Ile Gly Ala His Tyr Pro Gly           
225                 230                 235                 240           

aca cac acc gtg aag gac gcc aaa ctg acc aag aag aag ctg tgg tcc        768
Thr His Thr Val Lys Asp Ala Lys Leu Thr Lys Lys Lys Leu Trp Ser           
                245                 250                 255               

agc gag gac ttc agc acc ctg aat agt gac gtc gga gcc ggc tgc ctg        816
Ser Glu Asp Phe Ser Thr Leu Asn Ser Asp Val Gly Ala Gly Cys Leu           
            260                 265                 270                   

ggc aga atc ctg aac cag aac tac gtg aac ggc tac atg acc gcc aca        864
Gly Arg Ile Leu Asn Gln Asn Tyr Val Asn Gly Tyr Met Thr Ala Thr           
        275                 280                 285                       

atc gcc tgg aat ctg gtg gcc agc tac tac gag cag ctg ccc tac gga        912
Ile Ala Trp Asn Leu Val Ala Ser Tyr Tyr Glu Gln Leu Pro Tyr Gly           
    290                 295                 300                           

agg tgc ggc ctg atg aca gcc caa gag cct tgg agc gga cac tac gtg        960
Arg Cys Gly Leu Met Thr Ala Gln Glu Pro Trp Ser Gly His Tyr Val           
305                 310                 315                 320           

gtg gaa agc cct atc tgg gtg tca gcc cac acc aca cag ttc acc cag       1008
Val Glu Ser Pro Ile Trp Val Ser Ala His Thr Thr Gln Phe Thr Gln           
                325                 330                 335               

cct ggc tgg tac tac ctg aaa acc gtg ggc cac ctg gaa aaa ggc ggc       1056
Pro Gly Trp Tyr Tyr Leu Lys Thr Val Gly His Leu Glu Lys Gly Gly           
            340                 345                 350                   

tct tat gtg gcc ctg acc gac ggc ctg gga aac ctg aca atc atc gtg       1104
Ser Tyr Val Ala Leu Thr Asp Gly Leu Gly Asn Leu Thr Ile Ile Val           
        355                 360                 365                       

gaa acc atg agc cac aag cag agc gcc tgc atc aga ccc ttt ctg ccc       1152
Glu Thr Met Ser His Lys Gln Ser Ala Cys Ile Arg Pro Phe Leu Pro           
    370                 375                 380                           

tac ttc aac gtg tcc agg cag ttc gcc acc ttc gtg ctg aag ggc agc       1200
Tyr Phe Asn Val Ser Arg Gln Phe Ala Thr Phe Val Leu Lys Gly Ser           
385                 390                 395                 400           

ttc agc gag atc ccc gaa ctg caa gtg tgg tac acc aag ctg ggc aag       1248
Phe Ser Glu Ile Pro Glu Leu Gln Val Trp Tyr Thr Lys Leu Gly Lys           
                405                 410                 415               

ccc agc gag aga tac ctg ttt aag cag ctg gac agc ctg tgg ctg ctc       1296
Pro Ser Glu Arg Tyr Leu Phe Lys Gln Leu Asp Ser Leu Trp Leu Leu           
            420                 425                 430                   

gac agc agc tct acc ttc aca ctg gaa ctc caa gag gac gag atc ttc       1344
Asp Ser Ser Ser Thr Phe Thr Leu Glu Leu Gln Glu Asp Glu Ile Phe           
        435                 440                 445                       

acc ctg acc aca ctg acc gtg ggc agc aag gga tct tac cct ctg cct       1392
Thr Leu Thr Thr Leu Thr Val Gly Ser Lys Gly Ser Tyr Pro Leu Pro           
    450                 455                 460                           

cct aag agc gag ccc ttt cca cag atc tac gag gac gac ttc gac gtg       1440
Pro Lys Ser Glu Pro Phe Pro Gln Ile Tyr Glu Asp Asp Phe Asp Val           
465                 470                 475                 480           

gac tac cca ttc ttt agc gag gcc cct aac ttc gcc gat cag acc ggc       1488
Asp Tyr Pro Phe Phe Ser Glu Ala Pro Asn Phe Ala Asp Gln Thr Gly           
                485                 490                 495               

gtg ttc gag tac ttc acc aac atc gag gac ccc ggc gag cac agg ttt       1536
Val Phe Glu Tyr Phe Thr Asn Ile Glu Asp Pro Gly Glu His Arg Phe           
            500                 505                 510                   

acc ctg aga cag gtg ctg aac cag cgg cct att acc tgg gcc gct gac       1584
Thr Leu Arg Gln Val Leu Asn Gln Arg Pro Ile Thr Trp Ala Ala Asp           
        515                 520                 525                       

gcc tac aac acc atc tct atc atc ggc gac tac aag tgg tcc aac ctg       1632
Ala Tyr Asn Thr Ile Ser Ile Ile Gly Asp Tyr Lys Trp Ser Asn Leu           
    530                 535                 540                           

acc gtc aga tgc gac gtg tac atc gag aca ccc gag aaa ggc gga gtg       1680
Thr Val Arg Cys Asp Val Tyr Ile Glu Thr Pro Glu Lys Gly Gly Val           
545                 550                 555                 560           

ttt atc gcc ggc aga gtg aac aaa ggc ggc atc ctg atc aga agc gcc       1728
Phe Ile Ala Gly Arg Val Asn Lys Gly Gly Ile Leu Ile Arg Ser Ala           
                565                 570                 575               

agg gga atc ttc ttc tgg atc ttc gcc aac ggc acc tac aga gtg aca       1776
Arg Gly Ile Phe Phe Trp Ile Phe Ala Asn Gly Thr Tyr Arg Val Thr           
            580                 585                 590                   

ggc gat ctg gcc gga tgg gtc atc tat gcc ctg gga aga gtg gac gtg       1824
Gly Asp Leu Ala Gly Trp Val Ile Tyr Ala Leu Gly Arg Val Asp Val           
        595                 600                 605                       

acc gcc aag aag tgg tac act ctg acc ctg atc atc aag ggc aga ctg       1872
Thr Ala Lys Lys Trp Tyr Thr Leu Thr Leu Ile Ile Lys Gly Arg Leu           
    610                 615                 620                           

agc agc ggc atg ctg aat ggc aag acc gtg tgg aag aac atc ccc gtg       1920
Ser Ser Gly Met Leu Asn Gly Lys Thr Val Trp Lys Asn Ile Pro Val           
625                 630                 635                 640           

tct ttc ccc aaa aac ggc tgg gcc gcc atc gga acc cac agc ttc gaa       1968
Ser Phe Pro Lys Asn Gly Trp Ala Ala Ile Gly Thr His Ser Phe Glu           
                645                 650                 655               

ttt gcc cag ttc gac aac ttt cac gtg gaa gcc acc aga                   2007
Phe Ala Gln Phe Asp Asn Phe His Val Glu Ala Thr Arg                       
            660                 665                                       


<210>  8
<211>  669
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Synthetic Construct

<400>  8

Met Thr Ala Ala Ala Gly Ser Ala Gly His Ala Ala Val Pro Leu Leu 
1               5                   10                  15      


Leu Cys Ala Leu Leu Val Pro Gly Gly Ala Tyr Val Leu Asp Asp Ser 
            20                  25                  30          


Asp Gly Leu Gly Arg Glu Phe Asp Gly Val Gly Ala Val Ser Gly Gly 
        35                  40                  45              


Gly Ala Thr Ser Arg Leu Leu Val Asn Tyr Pro Glu Pro Tyr Arg Ser 
    50                  55                  60                  


Gln Ile Leu Asp Tyr Leu Phe Lys Pro Asn Phe Gly Ala Ser Leu His 
65                  70                  75                  80  


Ile Leu Lys Val Glu Ile Gly Gly Asp Gly Gln Thr Thr Asp Gly Thr 
                85                  90                  95      


Glu Pro Ser His Met His Tyr Ala Leu Asp Glu Asn Phe Phe Arg Gly 
            100                 105                 110         


Tyr Glu Trp Trp Leu Met Lys Glu Ala Lys Lys Arg Asn Pro Asn Ile 
        115                 120                 125             


Ile Leu Met Gly Leu Pro Trp Ser Phe Pro Gly Trp Ile Gly Lys Gly 
    130                 135                 140                 


Phe Asn Trp Pro Tyr Val Asn Leu Gln Leu Thr Ala Tyr Tyr Ile Met 
145                 150                 155                 160 


Thr Trp Ile Val Gly Ala Lys His Tyr His Asp Leu Asp Ile Asp Tyr 
                165                 170                 175     


Ile Gly Ile Trp Asn Glu Arg Ser Phe Asp Ile Asn Tyr Ile Lys Val 
            180                 185                 190         


Leu Arg Arg Met Leu Asn Tyr Gln Gly Leu Asp Arg Val Lys Ile Ile 
        195                 200                 205             


Ala Ser Asp Asn Leu Trp Glu Pro Ile Ser Ala Ser Met Leu Leu Asp 
    210                 215                 220                 


Ser Glu Leu Leu Lys Val Ile Asp Val Ile Gly Ala His Tyr Pro Gly 
225                 230                 235                 240 


Thr His Thr Val Lys Asp Ala Lys Leu Thr Lys Lys Lys Leu Trp Ser 
                245                 250                 255     


Ser Glu Asp Phe Ser Thr Leu Asn Ser Asp Val Gly Ala Gly Cys Leu 
            260                 265                 270         


Gly Arg Ile Leu Asn Gln Asn Tyr Val Asn Gly Tyr Met Thr Ala Thr 
        275                 280                 285             


Ile Ala Trp Asn Leu Val Ala Ser Tyr Tyr Glu Gln Leu Pro Tyr Gly 
    290                 295                 300                 


Arg Cys Gly Leu Met Thr Ala Gln Glu Pro Trp Ser Gly His Tyr Val 
305                 310                 315                 320 


Val Glu Ser Pro Ile Trp Val Ser Ala His Thr Thr Gln Phe Thr Gln 
                325                 330                 335     


Pro Gly Trp Tyr Tyr Leu Lys Thr Val Gly His Leu Glu Lys Gly Gly 
            340                 345                 350         


Ser Tyr Val Ala Leu Thr Asp Gly Leu Gly Asn Leu Thr Ile Ile Val 
        355                 360                 365             


Glu Thr Met Ser His Lys Gln Ser Ala Cys Ile Arg Pro Phe Leu Pro 
    370                 375                 380                 


Tyr Phe Asn Val Ser Arg Gln Phe Ala Thr Phe Val Leu Lys Gly Ser 
385                 390                 395                 400 


Phe Ser Glu Ile Pro Glu Leu Gln Val Trp Tyr Thr Lys Leu Gly Lys 
                405                 410                 415     


Pro Ser Glu Arg Tyr Leu Phe Lys Gln Leu Asp Ser Leu Trp Leu Leu 
            420                 425                 430         


Asp Ser Ser Ser Thr Phe Thr Leu Glu Leu Gln Glu Asp Glu Ile Phe 
        435                 440                 445             


Thr Leu Thr Thr Leu Thr Val Gly Ser Lys Gly Ser Tyr Pro Leu Pro 
    450                 455                 460                 


Pro Lys Ser Glu Pro Phe Pro Gln Ile Tyr Glu Asp Asp Phe Asp Val 
465                 470                 475                 480 


Asp Tyr Pro Phe Phe Ser Glu Ala Pro Asn Phe Ala Asp Gln Thr Gly 
                485                 490                 495     


Val Phe Glu Tyr Phe Thr Asn Ile Glu Asp Pro Gly Glu His Arg Phe 
            500                 505                 510         


Thr Leu Arg Gln Val Leu Asn Gln Arg Pro Ile Thr Trp Ala Ala Asp 
        515                 520                 525             


Ala Tyr Asn Thr Ile Ser Ile Ile Gly Asp Tyr Lys Trp Ser Asn Leu 
    530                 535                 540                 


Thr Val Arg Cys Asp Val Tyr Ile Glu Thr Pro Glu Lys Gly Gly Val 
545                 550                 555                 560 


Phe Ile Ala Gly Arg Val Asn Lys Gly Gly Ile Leu Ile Arg Ser Ala 
                565                 570                 575     


Arg Gly Ile Phe Phe Trp Ile Phe Ala Asn Gly Thr Tyr Arg Val Thr 
            580                 585                 590         


Gly Asp Leu Ala Gly Trp Val Ile Tyr Ala Leu Gly Arg Val Asp Val 
        595                 600                 605             


Thr Ala Lys Lys Trp Tyr Thr Leu Thr Leu Ile Ile Lys Gly Arg Leu 
    610                 615                 620                 


Ser Ser Gly Met Leu Asn Gly Lys Thr Val Trp Lys Asn Ile Pro Val 
625                 630                 635                 640 


Ser Phe Pro Lys Asn Gly Trp Ala Ala Ile Gly Thr His Ser Phe Glu 
                645                 650                 655     


Phe Ala Gln Phe Asp Asn Phe His Val Glu Ala Thr Arg 
            660                 665                 


<210>  9
<211>  2055
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Engineered human GALC coding sequence


<220>
<221>  CDS
<222>  (1)..(2055)

<220>
<221>  sig_peptide
<222>  (1)..(126)

<220>
<221>  mat
<222>  (127)..(2055)

<400>  9
atg gcc gag tgg ctg ctg tct gct agc tgg cag aga agg gcc aag gcc         48
Met Ala Glu Trp Leu Leu Ser Ala Ser Trp Gln Arg Arg Ala Lys Ala           
1               5                   10                  15                

atg aca gcc gcc gct gga tct gca ggc aga gct gct gtg cct ctg ctg         96
Met Thr Ala Ala Ala Gly Ser Ala Gly Arg Ala Ala Val Pro Leu Leu           
            20                  25                  30                    

ctg tgt gca ctg ctg gca cct ggc gga gcc tac gtg ctg gat gat tct        144
Leu Cys Ala Leu Leu Ala Pro Gly Gly Ala Tyr Val Leu Asp Asp Ser           
        35                  40                  45                        

gac ggc ctg ggc aga gag ttc gac ggc atc gga gct gtg tct ggc ggc        192
Asp Gly Leu Gly Arg Glu Phe Asp Gly Ile Gly Ala Val Ser Gly Gly           
    50                  55                  60                            

gga gcc aca agc aga ctg ctc gtg aac tac ccc gag ccc tac aga agc        240
Gly Ala Thr Ser Arg Leu Leu Val Asn Tyr Pro Glu Pro Tyr Arg Ser           
65                  70                  75                  80            

cag atc ctg gac tac ctg ttc aag ccc aac ttc ggc gcc agc ctg cac        288
Gln Ile Leu Asp Tyr Leu Phe Lys Pro Asn Phe Gly Ala Ser Leu His           
                85                  90                  95                

atc ctg aag gtg gaa atc ggc ggc gac ggc cag acc acc gat ggc aca        336
Ile Leu Lys Val Glu Ile Gly Gly Asp Gly Gln Thr Thr Asp Gly Thr           
            100                 105                 110                   

gaa cct agc cac atg cac tac gcc ctg gac gag aac tac ttc cgg ggc        384
Glu Pro Ser His Met His Tyr Ala Leu Asp Glu Asn Tyr Phe Arg Gly           
        115                 120                 125                       

tac gag tgg tgg ctg atg aag gaa gcc aag aag cgg aac ccc aac atc        432
Tyr Glu Trp Trp Leu Met Lys Glu Ala Lys Lys Arg Asn Pro Asn Ile           
    130                 135                 140                           

acc ctg atc ggc ctg cct tgg agc ttc cct ggc tgg ctg ggc aag ggc        480
Thr Leu Ile Gly Leu Pro Trp Ser Phe Pro Gly Trp Leu Gly Lys Gly           
145                 150                 155                 160           

ttc gac tgg cct tac gtg aac ctg cag ctg acc gcc tac tac gtc gtg        528
Phe Asp Trp Pro Tyr Val Asn Leu Gln Leu Thr Ala Tyr Tyr Val Val           
                165                 170                 175               

acc tgg atc gtg ggc gcc aag aga tac cac gac ctg gac atc gac tac        576
Thr Trp Ile Val Gly Ala Lys Arg Tyr His Asp Leu Asp Ile Asp Tyr           
            180                 185                 190                   

atc ggc atc tgg aac gag cgg agc tac aac gcc aac tac atc aag atc        624
Ile Gly Ile Trp Asn Glu Arg Ser Tyr Asn Ala Asn Tyr Ile Lys Ile           
        195                 200                 205                       

ctg cgg aag atg ctg aac tac cag ggc ctg cag aga gtg aag atc att        672
Leu Arg Lys Met Leu Asn Tyr Gln Gly Leu Gln Arg Val Lys Ile Ile           
    210                 215                 220                           

gcc agc gac aac ctg tgg gag agc atc agc gcc tcc atg ctg ctg gac        720
Ala Ser Asp Asn Leu Trp Glu Ser Ile Ser Ala Ser Met Leu Leu Asp           
225                 230                 235                 240           

gcc gag ctg ttc aag gtg gtg gat gtg atc ggc gcc cac tac cct ggc        768
Ala Glu Leu Phe Lys Val Val Asp Val Ile Gly Ala His Tyr Pro Gly           
                245                 250                 255               

aca cac tct gcc aag gac gcc aag ctg acc ggc aag aag ctg tgg tcc        816
Thr His Ser Ala Lys Asp Ala Lys Leu Thr Gly Lys Lys Leu Trp Ser           
            260                 265                 270                   

agc gag gac ttc agc acc ctg aac agc gac atg gga gcc ggc tgc tgg        864
Ser Glu Asp Phe Ser Thr Leu Asn Ser Asp Met Gly Ala Gly Cys Trp           
        275                 280                 285                       

ggc aga atc ctg aac cag aat tac atc aac ggc tac atg acc agc aca        912
Gly Arg Ile Leu Asn Gln Asn Tyr Ile Asn Gly Tyr Met Thr Ser Thr           
    290                 295                 300                           

atc gcc tgg aac ctg gtg gcc agc tac tac gag cag ctg ccc tac ggc        960
Ile Ala Trp Asn Leu Val Ala Ser Tyr Tyr Glu Gln Leu Pro Tyr Gly           
305                 310                 315                 320           

aga tgc ggc ctg atg aca gcc cag gaa cct tgg agc ggc cac tac gtg       1008
Arg Cys Gly Leu Met Thr Ala Gln Glu Pro Trp Ser Gly His Tyr Val           
                325                 330                 335               

gtg gaa agc cct gtg tgg gtg tcc gcc cac acc acc cag ttt aca cag       1056
Val Glu Ser Pro Val Trp Val Ser Ala His Thr Thr Gln Phe Thr Gln           
            340                 345                 350                   

ccc ggc tgg tac tac ctg aaa acc gtg ggc cac ctg gaa aag ggc ggc       1104
Pro Gly Trp Tyr Tyr Leu Lys Thr Val Gly His Leu Glu Lys Gly Gly           
        355                 360                 365                       

agc tat gtg gcc ctg aca gac gga ctg ggc aac ctg acc atc atc atc       1152
Ser Tyr Val Ala Leu Thr Asp Gly Leu Gly Asn Leu Thr Ile Ile Ile           
    370                 375                 380                           

gag aca atg tcc cac aag cac agc aag tgc atc aga ccc ttt ctg ccc       1200
Glu Thr Met Ser His Lys His Ser Lys Cys Ile Arg Pro Phe Leu Pro           
385                 390                 395                 400           

tac ttc aac gtg tcc cag cag ttc gcc acc ttc gtg ctg aag ggc agc       1248
Tyr Phe Asn Val Ser Gln Gln Phe Ala Thr Phe Val Leu Lys Gly Ser           
                405                 410                 415               

ttc agc gag atc ccc gaa ctg caa gtg tgg tac acc aag ctg gga aag       1296
Phe Ser Glu Ile Pro Glu Leu Gln Val Trp Tyr Thr Lys Leu Gly Lys           
            420                 425                 430                   

acc agc gag cgg ttc ctg ttt aag cag ctg gac agc ctg tgg ctg ctg       1344
Thr Ser Glu Arg Phe Leu Phe Lys Gln Leu Asp Ser Leu Trp Leu Leu           
        435                 440                 445                       

gac agc gac ggc agc ttt acc ctg agc ctg cac gag gac gag ctg ttt       1392
Asp Ser Asp Gly Ser Phe Thr Leu Ser Leu His Glu Asp Glu Leu Phe           
    450                 455                 460                           

aca ctg acc acc ctg acc aca ggc cgg aag ggc tct tac ccc ctg cct       1440
Thr Leu Thr Thr Leu Thr Thr Gly Arg Lys Gly Ser Tyr Pro Leu Pro           
465                 470                 475                 480           

cct aag agc cag ccc ttc cca agc acc tac aag gac gac ttc aat gtg       1488
Pro Lys Ser Gln Pro Phe Pro Ser Thr Tyr Lys Asp Asp Phe Asn Val           
                485                 490                 495               

gac tac cca ttc ttt agc gag gcc ccc aat ttc gcc gac cag acc ggc       1536
Asp Tyr Pro Phe Phe Ser Glu Ala Pro Asn Phe Ala Asp Gln Thr Gly           
            500                 505                 510                   

gtg ttc gag tac ttc acc aac atc gag gac ccc ggc gag cac cac ttc       1584
Val Phe Glu Tyr Phe Thr Asn Ile Glu Asp Pro Gly Glu His His Phe           
        515                 520                 525                       

acc ctg aga cag gtg ctg aac cag cgg ccc atc acc tgg gct gcc gat       1632
Thr Leu Arg Gln Val Leu Asn Gln Arg Pro Ile Thr Trp Ala Ala Asp           
    530                 535                 540                           

gcc agc aac acc atc agc atc atc ggc gac tac aac tgg acc aat ctg       1680
Ala Ser Asn Thr Ile Ser Ile Ile Gly Asp Tyr Asn Trp Thr Asn Leu           
545                 550                 555                 560           

aca atc aag tgc gac gtg tac atc gaa acc ccc gac acc ggc gga gtg       1728
Thr Ile Lys Cys Asp Val Tyr Ile Glu Thr Pro Asp Thr Gly Gly Val           
                565                 570                 575               

ttt atc gcc ggc aga gtg aac aag ggc ggg atc ctg atc aga agc gcc       1776
Phe Ile Ala Gly Arg Val Asn Lys Gly Gly Ile Leu Ile Arg Ser Ala           
            580                 585                 590                   

aga ggc atc ttc ttt tgg atc ttc gcc aac ggc agc tac aga gtg acc       1824
Arg Gly Ile Phe Phe Trp Ile Phe Ala Asn Gly Ser Tyr Arg Val Thr           
        595                 600                 605                       

ggc gat ctg gcc ggc tgg atc atc tac gct ctg ggc agg gtg gaa gtg       1872
Gly Asp Leu Ala Gly Trp Ile Ile Tyr Ala Leu Gly Arg Val Glu Val           
    610                 615                 620                           

acc gcc aag aaa tgg tac acc ctg aca ctg act atc aag ggc cac ttc       1920
Thr Ala Lys Lys Trp Tyr Thr Leu Thr Leu Thr Ile Lys Gly His Phe           
625                 630                 635                 640           

gcc tcc ggc atg ctg aac gac aag agc ctg tgg acc gac atc ccc gtg       1968
Ala Ser Gly Met Leu Asn Asp Lys Ser Leu Trp Thr Asp Ile Pro Val           
                645                 650                 655               

aac ttc ccc aag aat ggc tgg gcc gcc atc ggc acc cac agc ttt gag       2016
Asn Phe Pro Lys Asn Gly Trp Ala Ala Ile Gly Thr His Ser Phe Glu           
            660                 665                 670                   

ttc gcc cag ttc gac aac ttc ctg gtg gaa gcc acc aga                   2055
Phe Ala Gln Phe Asp Asn Phe Leu Val Glu Ala Thr Arg                       
        675                 680                 685                       


<210>  10
<211>  685
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Synthetic Construct

<400>  10

Met Ala Glu Trp Leu Leu Ser Ala Ser Trp Gln Arg Arg Ala Lys Ala 
1               5                   10                  15      


Met Thr Ala Ala Ala Gly Ser Ala Gly Arg Ala Ala Val Pro Leu Leu 
            20                  25                  30          


Leu Cys Ala Leu Leu Ala Pro Gly Gly Ala Tyr Val Leu Asp Asp Ser 
        35                  40                  45              


Asp Gly Leu Gly Arg Glu Phe Asp Gly Ile Gly Ala Val Ser Gly Gly 
    50                  55                  60                  


Gly Ala Thr Ser Arg Leu Leu Val Asn Tyr Pro Glu Pro Tyr Arg Ser 
65                  70                  75                  80  


Gln Ile Leu Asp Tyr Leu Phe Lys Pro Asn Phe Gly Ala Ser Leu His 
                85                  90                  95      


Ile Leu Lys Val Glu Ile Gly Gly Asp Gly Gln Thr Thr Asp Gly Thr 
            100                 105                 110         


Glu Pro Ser His Met His Tyr Ala Leu Asp Glu Asn Tyr Phe Arg Gly 
        115                 120                 125             


Tyr Glu Trp Trp Leu Met Lys Glu Ala Lys Lys Arg Asn Pro Asn Ile 
    130                 135                 140                 


Thr Leu Ile Gly Leu Pro Trp Ser Phe Pro Gly Trp Leu Gly Lys Gly 
145                 150                 155                 160 


Phe Asp Trp Pro Tyr Val Asn Leu Gln Leu Thr Ala Tyr Tyr Val Val 
                165                 170                 175     


Thr Trp Ile Val Gly Ala Lys Arg Tyr His Asp Leu Asp Ile Asp Tyr 
            180                 185                 190         


Ile Gly Ile Trp Asn Glu Arg Ser Tyr Asn Ala Asn Tyr Ile Lys Ile 
        195                 200                 205             


Leu Arg Lys Met Leu Asn Tyr Gln Gly Leu Gln Arg Val Lys Ile Ile 
    210                 215                 220                 


Ala Ser Asp Asn Leu Trp Glu Ser Ile Ser Ala Ser Met Leu Leu Asp 
225                 230                 235                 240 


Ala Glu Leu Phe Lys Val Val Asp Val Ile Gly Ala His Tyr Pro Gly 
                245                 250                 255     


Thr His Ser Ala Lys Asp Ala Lys Leu Thr Gly Lys Lys Leu Trp Ser 
            260                 265                 270         


Ser Glu Asp Phe Ser Thr Leu Asn Ser Asp Met Gly Ala Gly Cys Trp 
        275                 280                 285             


Gly Arg Ile Leu Asn Gln Asn Tyr Ile Asn Gly Tyr Met Thr Ser Thr 
    290                 295                 300                 


Ile Ala Trp Asn Leu Val Ala Ser Tyr Tyr Glu Gln Leu Pro Tyr Gly 
305                 310                 315                 320 


Arg Cys Gly Leu Met Thr Ala Gln Glu Pro Trp Ser Gly His Tyr Val 
                325                 330                 335     


Val Glu Ser Pro Val Trp Val Ser Ala His Thr Thr Gln Phe Thr Gln 
            340                 345                 350         


Pro Gly Trp Tyr Tyr Leu Lys Thr Val Gly His Leu Glu Lys Gly Gly 
        355                 360                 365             


Ser Tyr Val Ala Leu Thr Asp Gly Leu Gly Asn Leu Thr Ile Ile Ile 
    370                 375                 380                 


Glu Thr Met Ser His Lys His Ser Lys Cys Ile Arg Pro Phe Leu Pro 
385                 390                 395                 400 


Tyr Phe Asn Val Ser Gln Gln Phe Ala Thr Phe Val Leu Lys Gly Ser 
                405                 410                 415     


Phe Ser Glu Ile Pro Glu Leu Gln Val Trp Tyr Thr Lys Leu Gly Lys 
            420                 425                 430         


Thr Ser Glu Arg Phe Leu Phe Lys Gln Leu Asp Ser Leu Trp Leu Leu 
        435                 440                 445             


Asp Ser Asp Gly Ser Phe Thr Leu Ser Leu His Glu Asp Glu Leu Phe 
    450                 455                 460                 


Thr Leu Thr Thr Leu Thr Thr Gly Arg Lys Gly Ser Tyr Pro Leu Pro 
465                 470                 475                 480 


Pro Lys Ser Gln Pro Phe Pro Ser Thr Tyr Lys Asp Asp Phe Asn Val 
                485                 490                 495     


Asp Tyr Pro Phe Phe Ser Glu Ala Pro Asn Phe Ala Asp Gln Thr Gly 
            500                 505                 510         


Val Phe Glu Tyr Phe Thr Asn Ile Glu Asp Pro Gly Glu His His Phe 
        515                 520                 525             


Thr Leu Arg Gln Val Leu Asn Gln Arg Pro Ile Thr Trp Ala Ala Asp 
    530                 535                 540                 


Ala Ser Asn Thr Ile Ser Ile Ile Gly Asp Tyr Asn Trp Thr Asn Leu 
545                 550                 555                 560 


Thr Ile Lys Cys Asp Val Tyr Ile Glu Thr Pro Asp Thr Gly Gly Val 
                565                 570                 575     


Phe Ile Ala Gly Arg Val Asn Lys Gly Gly Ile Leu Ile Arg Ser Ala 
            580                 585                 590         


Arg Gly Ile Phe Phe Trp Ile Phe Ala Asn Gly Ser Tyr Arg Val Thr 
        595                 600                 605             


Gly Asp Leu Ala Gly Trp Ile Ile Tyr Ala Leu Gly Arg Val Glu Val 
    610                 615                 620                 


Thr Ala Lys Lys Trp Tyr Thr Leu Thr Leu Thr Ile Lys Gly His Phe 
625                 630                 635                 640 


Ala Ser Gly Met Leu Asn Asp Lys Ser Leu Trp Thr Asp Ile Pro Val 
                645                 650                 655     


Asn Phe Pro Lys Asn Gly Trp Ala Ala Ile Gly Thr His Ser Phe Glu 
            660                 665                 670         


Phe Ala Gln Phe Asp Asn Phe Leu Val Glu Ala Thr Arg 
        675                 680                 685 


<210>  11
<211>  130
<212>  DNA
<213>  Artificial sequence

<220>
<223>  AAV2 - 5' ITR

<400>  11
ctgcgcgctc gctcgctcac tgaggccgcc cgggcaaagc ccgggcgtcg ggcgaccttt       60

ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact      120

aggggttcct                                                             130


<210>  12
<211>  382
<212>  DNA
<213>  Artificial sequence

<220>
<223>  CMV IE enhancer

<400>  12
ctagtcgaca ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc       60

atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac      120

cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa      180

tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag      240

tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc      300

ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct      360

acgtattagt catcgctatt ac                                               382


<210>  13
<211>  282
<212>  DNA
<213>  Artificial sequence

<220>
<223>  CB promoter

<400>  13
tggtcgaggt gagccccacg ttctgcttca ctctccccat ctcccccccc tccccacccc       60

caattttgta tttatttatt ttttaattat tttgtgcagc gatgggggcg gggggggggg      120

gggggcgcgc gccaggcggg gcggggcggg gcgaggggcg gggcggggcg aggcggagag      180

gtgcggcggc agccaatcag agcggcgcgc tccgaaagtt tccttttatg gcgaggcggc      240

ggcggcggcg gccctataaa aagcgaagcg cgcggcgggc gg                         282


<210>  14
<211>  666
<212>  DNA
<213>  Artificial sequence

<220>
<223>  CB7 promoter

<400>  14
ctagtcgaca ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc       60

atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac      120

cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa      180

tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag      240

tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc      300

ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct      360

acgtattagt catcgctatt accatggtcg aggtgagccc cacgttctgc ttcactctcc      420

ccatctcccc cccctcccca cccccaattt tgtatttatt tattttttaa ttattttgtg      480

cagcgatggg ggcggggggg gggggggggc gcgcgccagg cggggcgggg cggggcgagg      540

ggcggggcgg ggcgaggcgg agaggtgcgg cggcagccaa tcagagcggc gcgctccgaa      600

agtttccttt tatggcgagg cggcggcggc ggcggcccta taaaaagcga agcgcgcggc      660

gggcgg                                                                 666


<210>  15
<211>  972
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  chimeric intron

<400>  15
gtgagcgggc gggacggccc ttctcctccg ggctgtaatt agcgcttggt ttaatgacgg       60

cttgtttctt ttctgtggct gcgtgaaagc cttgaggggc tccgggaggg ccctttgtgc      120

ggggggagcg gctcgggggg tgcgtgcgtg tgtgtgtgcg tggggagcgc cgcgtgcggc      180

tccgcgctgc ccggcggctg tgagcgctgc gggcgcggcg cggggctttg tgcgctccgc      240

agtgtgcgcg aggggagcgc ggccgggggc ggtgccccgc ggtgcggggg gggctgcgag      300

gggaacaaag gctgcgtgcg gggtgtgtgc gtgggggggt gagcaggggg tgtgggcgcg      360

tcggtcgggc tgcaaccccc cctgcacccc cctccccgag ttgctgagca cggcccggct      420

tcgggtgcgg ggctccgtac ggggcgtggc gcggggctcg ccgtgccggg cggggggtgg      480

cggcaggtgg gggtgccggg cggggcgggg ccgcctcggg ccggggaggg ctcgggggag      540

gggcgcggcg gcccccggag cgccggcggc tgtcgaggcg cggcgagccg cagccattgc      600

cttttatggt aatcgtgcga gagggcgcag ggacttcctt tgtcccaaat ctgtgcggag      660

ccgaaatctg ggaggcgccg ccgcaccccc tctagcgggc gcggggcgaa gcggtgcggc      720

gccggcagga aggaaatggg cggggagggc cttcgtgcgt cgccgcgccg ccgtcccctt      780

ctccctctcc agcctcgggg ctgtccgcgg ggggacggct gccttcgggg gggacggggc      840

agggcggggt tcggcttctg gcgtgtgacc ggcggctcta gagcctctgc taaccatgtt      900

catgccttct tctttttcct acagctcctg ggcaacgtgc tggttattgt gctgtctcat      960

cattttggca aa                                                          972


<210>  16
<211>  127
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Rabbit globin polyA

<400>  16
gatctttttc cctctgccaa aaattatggg gacatcatga agccccttga gcatctgact       60

tctggctaat aaaggaaatt tattttcatt gcaatagtgt gttggaattt tttgtgtctc      120

tcactcg                                                                127


<210>  17
<211>  130
<212>  DNA
<213>  Artificial sequence

<220>
<223>  AAV2 - 3' ITR

<400>  17
aggaacccct agtgatggag ttggccactc cctctctgcg cgctcgctcg ctcactgagg       60

ccgggcgacc aaaggtcgcc cgacgcccgg gctttgcccg ggcggcctca gtgagcgagc      120

gagcgcgcag                                                             130


<210>  18
<211>  4337
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Vector genome with canine GALC

<400>  18
ctgcgcgctc gctcgctcac tgaggccgcc cgggcaaagc ccgggcgtcg ggcgaccttt       60

ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact      120

aggggttcct tgtagttaat gattaacccg ccatgctact tatctaccag ggtaatgggg      180

atcctctaga actatagcta gtcgacattg attattgact agttattaat agtaatcaat      240

tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa      300

tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt      360

tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta      420

aactgcccac ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt      480

caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc      540

tacttggcag tacatctacg tattagtcat cgctattacc atggtcgagg tgagccccac      600

gttctgcttc actctcccca tctccccccc ctccccaccc ccaattttgt atttatttat      660

tttttaatta ttttgtgcag cgatgggggc gggggggggg ggggggcgcg cgccaggcgg      720

ggcggggcgg ggcgaggggc ggggcggggc gaggcggaga ggtgcggcgg cagccaatca      780

gagcggcgcg ctccgaaagt ttccttttat ggcgaggcgg cggcggcggc ggccctataa      840

aaagcgaagc gcgcggcggg cgggagtcgc tgcgcgctgc cttcgccccg tgccccgctc      900

cgccgccgcc tcgcgccgcc cgccccggct ctgactgacc gcgttactcc cacaggtgag      960

cgggcgggac ggcccttctc ctccgggctg taattagcgc ttggtttaat gacggcttgt     1020

ttcttttctg tggctgcgtg aaagccttga ggggctccgg gagggccctt tgtgcggggg     1080

gagcggctcg gggggtgcgt gcgtgtgtgt gtgcgtgggg agcgccgcgt gcggctccgc     1140

gctgcccggc ggctgtgagc gctgcgggcg cggcgcgggg ctttgtgcgc tccgcagtgt     1200

gcgcgagggg agcgcggccg ggggcggtgc cccgcggtgc ggggggggct gcgaggggaa     1260

caaaggctgc gtgcggggtg tgtgcgtggg ggggtgagca gggggtgtgg gcgcgtcggt     1320

cgggctgcaa ccccccctgc acccccctcc ccgagttgct gagcacggcc cggcttcggg     1380

tgcggggctc cgtacggggc gtggcgcggg gctcgccgtg ccgggcgggg ggtggcggca     1440

ggtgggggtg ccgggcgggg cggggccgcc tcgggccggg gagggctcgg gggaggggcg     1500

cggcggcccc cggagcgccg gcggctgtcg aggcgcggcg agccgcagcc attgcctttt     1560

atggtaatcg tgcgagaggg cgcagggact tcctttgtcc caaatctgtg cggagccgaa     1620

atctgggagg cgccgccgca ccccctctag cgggcgcggg gcgaagcggt gcggcgccgg     1680

caggaaggaa atgggcgggg agggccttcg tgcgtcgccg cgccgccgtc cccttctccc     1740

tctccagcct cggggctgtc cgcgggggga cggctgcctt cgggggggac ggggcagggc     1800

ggggttcggc ttctggcgtg tgaccggcgg ctctagagcc tctgctaacc atgttcatgc     1860

cttcttcttt ttcctacagc tcctgggcaa cgtgctggtt attgtgctgt ctcatcattt     1920

tggcaaagaa ttcgccacca tgacagccgc tgctggatct gctggacacg ctgctgttcc     1980

tctgctgctg tgtgcactgc tggtgcctgg cggagcttac gtgctggatg attctgatgg     2040

actaggccgc gagttcgacg gcgtgggagc tgtttctggt ggcggagcca catctaggct     2100

gctggtcaat taccccgagc cttacaggtc ccagatcctg gactacctgt tcaagcccaa     2160

cttcggcgcc agcctgcaca tcctgaaggt ggaaattggc ggcgacggcc agaccaccga     2220

cggaacagaa cctagccaca tgcactacgc cctggacgag aacttcttca ggggctacga     2280

gtggtggctg atgaaggaag ccaagaagag gaaccccaac atcatcctga tgggcctgcc     2340

ttggtctttc cctggctgga tcggcaaggg cttcaactgg ccttacgtga acctgcagct     2400

gaccgcctac tacatcatga cctggatcgt gggcgccaag cactaccacg acctggacat     2460

cgactacatc ggcatctgga acgagcggag cttcgacatc aattacatca aggtgctgcg     2520

gcggatgctg aactaccagg gcctcgacag agtgaagatc attgccagcg acaacctgtg     2580

ggagcccatc agcgcttcta tgctgctgga ctccgagctg ctgaaagtga tcgacgtgat     2640

cggcgctcac taccccggaa cacacaccgt gaaggacgcc aaactgacca agaagaagct     2700

gtggtccagc gaggacttca gcaccctgaa tagtgacgtc ggagccggct gcctgggcag     2760

aatcctgaac cagaactacg tgaacggcta catgaccgcc acaatcgcct ggaatctggt     2820

ggccagctac tacgagcagc tgccctacgg aaggtgcggc ctgatgacag cccaagagcc     2880

ttggagcgga cactacgtgg tggaaagccc tatctgggtg tcagcccaca ccacacagtt     2940

cacccagcct ggctggtact acctgaaaac cgtgggccac ctggaaaaag gcggctctta     3000

tgtggccctg accgacggcc tgggaaacct gacaatcatc gtggaaacca tgagccacaa     3060

gcagagcgcc tgcatcagac cctttctgcc ctacttcaac gtgtccaggc agttcgccac     3120

cttcgtgctg aagggcagct tcagcgagat ccccgaactg caagtgtggt acaccaagct     3180

gggcaagccc agcgagagat acctgtttaa gcagctggac agcctgtggc tgctcgacag     3240

cagctctacc ttcacactgg aactccaaga ggacgagatc ttcaccctga ccacactgac     3300

cgtgggcagc aagggatctt accctctgcc tcctaagagc gagccctttc cacagatcta     3360

cgaggacgac ttcgacgtgg actacccatt ctttagcgag gcccctaact tcgccgatca     3420

gaccggcgtg ttcgagtact tcaccaacat cgaggacccc ggcgagcaca ggtttaccct     3480

gagacaggtg ctgaaccagc ggcctattac ctgggccgct gacgcctaca acaccatctc     3540

tatcatcggc gactacaagt ggtccaacct gaccgtcaga tgcgacgtgt acatcgagac     3600

acccgagaaa ggcggagtgt ttatcgccgg cagagtgaac aaaggcggca tcctgatcag     3660

aagcgccagg ggaatcttct tctggatctt cgccaacggc acctacagag tgacaggcga     3720

tctggccgga tgggtcatct atgccctggg aagagtggac gtgaccgcca agaagtggta     3780

cactctgacc ctgatcatca agggcagact gagcagcggc atgctgaatg gcaagaccgt     3840

gtggaagaac atccccgtgt ctttccccaa aaacggctgg gccgccatcg gaacccacag     3900

cttcgaattt gcccagttcg acaactttca cgtggaagcc accagatgat aacctcaggc     3960

tcgaggacgg ggtgaactac gcctgaggat ccgatctttt tccctctgcc aaaaattatg     4020

gggacatcat gaagcccctt gagcatctga cttctggcta ataaaggaaa tttattttca     4080

ttgcaatagt gtgttggaat tttttgtgtc tctcactcgg aagcaattcg ttgatctgaa     4140

tttcgaccac ccataatacc cattaccctg gtagataagt agcatggcgg gttaatcatt     4200

aactacaagg aacccctagt gatggagttg gccactccct ctctgcgcgc tcgctcgctc     4260

actgaggccg ggcgaccaaa ggtcgcccga cgcccgggct ttgcccgggc ggcctcagtg     4320

agcgagcgag cgcgcag                                                    4337


<210>  19
<211>  4386
<212>  DNA
<213>  Artificial sequence

<220>
<223>  CB7.CI.hGALC.rBG


<220>
<221>  misc_feature
<222>  (1)..(130)
<223>  5' ITR

<220>
<221>  misc_feature
<222>  (198)..(579)
<223>  CMV IE Enhancer

<220>
<221>  misc_feature
<222>  (582)..(863)
<223>  CB promoter

<220>
<221>  misc_feature
<222>  (836)..(839)
<223>  TATA

<220>
<221>  misc_feature
<222>  (958)..(1930)
<223>  chimeric intron

<220>
<221>  misc_feature
<222>  (1948)..(4002)
<223>  hGALCco

<220>
<221>  misc_feature
<222>  (4042)..(4168)
<223>  Rabbit globin poly A

<220>
<221>  misc_feature
<222>  (4257)..(4386)
<223>  3' ITR

<400>  19
ctgcgcgctc gctcgctcac tgaggccgcc cgggcaaagc ccgggcgtcg ggcgaccttt       60

ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact      120

aggggttcct tgtagttaat gattaacccg ccatgctact tatctaccag ggtaatgggg      180

atcctctaga actatagcta gtcgacattg attattgact agttattaat agtaatcaat      240

tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa      300

tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt      360

tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggact atttacggta      420

aactgcccac ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt      480

caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc      540

tacttggcag tacatctacg tattagtcat cgctattacc atggtcgagg tgagccccac      600

gttctgcttc actctcccca tctccccccc ctccccaccc ccaattttgt atttatttat      660

tttttaatta ttttgtgcag cgatgggggc gggggggggg ggggggcgcg cgccaggcgg      720

ggcggggcgg ggcgaggggc ggggcggggc gaggcggaga ggtgcggcgg cagccaatca      780

gagcggcgcg ctccgaaagt ttccttttat ggcgaggcgg cggcggcggc ggccctataa      840

aaagcgaagc gcgcggcggg cggggagtcg ctgcgacgct gccttcgccc cgtgccccgc      900

tccgccgccg cctcgcgccg cccgccccgg ctctgactga ccgcgttact cccacaggtg      960

agcgggcggg acggcccttc tcctccgggc tgtaattagc gcttggttta atgacggctt     1020

gtttcttttc tgtggctgcg tgaaagcctt gaggggctcc gggagggccc tttgtgcggg     1080

gggagcggct cggggggtgc gtgcgtgtgt gtgtgcgtgg ggagcgccgc gtgcggctcc     1140

gcgctgcccg gcggctgtga gcgctgcggg cgcggcgcgg ggctttgtgc gctccgcagt     1200

gtgcgcgagg ggagcgcggc cgggggcggt gccccgcggt gcgggggggg ctgcgagggg     1260

aacaaaggct gcgtgcgggg tgtgtgcgtg ggggggtgag cagggggtgt gggcgcgtcg     1320

gtcgggctgc aaccccccct gcacccccct ccccgagttg ctgagcacgg cccggcttcg     1380

ggtgcggggc tccgtacggg gcgtggcgcg gggctcgccg tgccgggcgg ggggtggcgg     1440

caggtggggg tgccgggcgg ggcggggccg cctcgggccg gggagggctc gggggagggg     1500

cgcggcggcc cccggagcgc cggcggctgt cgaggcgcgg cgagccgcag ccattgcctt     1560

ttatggtaat cgtgcgagag ggcgcaggga cttcctttgt cccaaatctg tgcggagccg     1620

aaatctggga ggcgccgccg caccccctct agcgggcgcg gggcgaagcg gtgcggcgcc     1680

ggcaggaagg aaatgggcgg ggagggcctt cgtgcgtcgc cgcgccgccg tccccttctc     1740

cctctccagc ctcggggctg tccgcggggg gacggctgcc ttcggggggg acggggcagg     1800

gcggggttcg gcttctggcg tgtgaccggc ggctctagag cctctgctaa ccatgttcat     1860

gccttcttct ttttcctaca gctcctgggc aacgtgctgg ttattgtgct gtctcatcat     1920

tttggcaaag aattcacgcg tgccaccatg gccgagtggc tgctgtctgc tagctggcag     1980

agaagggcca aggccatgac agccgccgct ggatctgcag gcagagctgc tgtgcctctg     2040

ctgctgtgtg cactgctggc acctggcgga gcctacgtgc tggatgattc tgacggcctg     2100

ggcagagagt tcgacggcat cggagctgtg tctggcggcg gagccacaag cagactgctc     2160

gtgaactacc ccgagcccta cagaagccag atcctggact acctgttcaa gcccaacttc     2220

ggcgccagcc tgcacatcct gaaggtggaa atcggcggcg acggccagac caccgatggc     2280

acagaaccta gccacatgca ctacgccctg gacgagaact acttccgggg ctacgagtgg     2340

tggctgatga aggaagccaa gaagcggaac cccaacatca ccctgatcgg cctgccttgg     2400

agcttccctg gctggctggg caagggcttc gactggcctt acgtgaacct gcagctgacc     2460

gcctactacg tcgtgacctg gatcgtgggc gccaagagat accacgacct ggacatcgac     2520

tacatcggca tctggaacga gcggagctac aacgccaact acatcaagat cctgcggaag     2580

atgctgaact accagggcct gcagagagtg aagatcattg ccagcgacaa cctgtgggag     2640

agcatcagcg cctccatgct gctggacgcc gagctgttca aggtggtgga tgtgatcggc     2700

gcccactacc ctggcacaca ctctgccaag gacgccaagc tgaccggcaa gaagctgtgg     2760

tccagcgagg acttcagcac cctgaacagc gacatgggag ccggctgctg gggcagaatc     2820

ctgaaccaga attacatcaa cggctacatg accagcacaa tcgcctggaa cctggtggcc     2880

agctactacg agcagctgcc ctacggcaga tgcggcctga tgacagccca ggaaccttgg     2940

agcggccact acgtggtgga aagccctgtg tgggtgtccg cccacaccac ccagtttaca     3000

cagcccggct ggtactacct gaaaaccgtg ggccacctgg aaaagggcgg cagctatgtg     3060

gccctgacag acggactggg caacctgacc atcatcatcg agacaatgtc ccacaagcac     3120

agcaagtgca tcagaccctt tctgccctac ttcaacgtgt cccagcagtt cgccaccttc     3180

gtgctgaagg gcagcttcag cgagatcccc gaactgcaag tgtggtacac caagctggga     3240

aagaccagcg agcggttcct gtttaagcag ctggacagcc tgtggctgct ggacagcgac     3300

ggcagcttta ccctgagcct gcacgaggac gagctgttta cactgaccac cctgaccaca     3360

ggccggaagg gctcttaccc cctgcctcct aagagccagc ccttcccaag cacctacaag     3420

gacgacttca atgtggacta cccattcttt agcgaggccc ccaatttcgc cgaccagacc     3480

ggcgtgttcg agtacttcac caacatcgag gaccccggcg agcaccactt caccctgaga     3540

caggtgctga accagcggcc catcacctgg gctgccgatg ccagcaacac catcagcatc     3600

atcggcgact acaactggac caatctgaca atcaagtgcg acgtgtacat cgaaaccccc     3660

gacaccggcg gagtgtttat cgccggcaga gtgaacaagg gcgggatcct gatcagaagc     3720

gccagaggca tcttcttttg gatcttcgcc aacggcagct acagagtgac cggcgatctg     3780

gccggctgga tcatctacgc tctgggcagg gtggaagtga ccgccaagaa atggtacacc     3840

ctgacactga ctatcaaggg ccacttcgcc tccggcatgc tgaacgacaa gagcctgtgg     3900

accgacatcc ccgtgaactt ccccaagaat ggctgggccg ccatcggcac ccacagcttt     3960

gagttcgccc agttcgacaa cttcctggtg gaagccacca gatgatgact cgaggacggg     4020

gtgaactacg cctgaggatc cgatcttttt ccctctgcca aaaattatgg ggacatcatg     4080

aagccccttg agcatctgac ttctggctaa taaaggaaat ttattttcat tgcaatagtg     4140

tgttggaatt ttttgtgtct ctcactcgga agcaattcgt tgatctgaat ttcgaccacc     4200

cataataccc attaccctgg tagataagta gcatggcggg ttaatcatta actacaagga     4260

acccctagtg atggagttgg ccactccctc tctgcgcgct cgctcgctca ctgaggccgg     4320

gcgaccaaag gtcgcccgac gcccgggctt tgcccgggcg gcctcagtga gcgagcgagc     4380

gcgcag                                                                4386


<210>  20
<211>  2208
<212>  DNA
<213>  Artificial sequence

<220>
<223>  AAV1 VP1


<220>
<221>  CDS
<222>  (1)..(2208)

<400>  20
atg gct gcc gat ggt tat ctt cca gat tgg ctc gag gac aac ctc tct         48
Met Ala Ala Asp Gly Tyr Leu Pro Asp Trp Leu Glu Asp Asn Leu Ser           
1               5                   10                  15                

gag ggc att cgc gag tgg tgg gac ttg aaa cct gga gcc ccg aag ccc         96
Glu Gly Ile Arg Glu Trp Trp Asp Leu Lys Pro Gly Ala Pro Lys Pro           
            20                  25                  30                    

aaa gcc aac cag caa aag cag gac gac ggc cgg ggt ctg gtg ctt cct        144
Lys Ala Asn Gln Gln Lys Gln Asp Asp Gly Arg Gly Leu Val Leu Pro           
        35                  40                  45                        

ggc tac aag tac ctc gga ccc ttc aac gga ctc gac aag ggg gag ccc        192
Gly Tyr Lys Tyr Leu Gly Pro Phe Asn Gly Leu Asp Lys Gly Glu Pro           
    50                  55                  60                            

gtc aac gcg gcg gac gca gcg gcc ctc gag cac gac aag gcc tac gac        240
Val Asn Ala Ala Asp Ala Ala Ala Leu Glu His Asp Lys Ala Tyr Asp           
65                  70                  75                  80            

cag cag ctc aaa gcg ggt gac aat ccg tac ctg cgg tat aac cac gcc        288
Gln Gln Leu Lys Ala Gly Asp Asn Pro Tyr Leu Arg Tyr Asn His Ala           
                85                  90                  95                

gac gcc gag ttt cag gag cgt ctg caa gaa gat acg tct ttt ggg ggc        336
Asp Ala Glu Phe Gln Glu Arg Leu Gln Glu Asp Thr Ser Phe Gly Gly           
            100                 105                 110                   

aac ctc ggg cga gca gtc ttc cag gcc aag aag cgg gtt ctc gaa cct        384
Asn Leu Gly Arg Ala Val Phe Gln Ala Lys Lys Arg Val Leu Glu Pro           
        115                 120                 125                       

ctc ggt ctg gtt gag gaa ggc gct aag acg gct cct gga aag aaa cgt        432
Leu Gly Leu Val Glu Glu Gly Ala Lys Thr Ala Pro Gly Lys Lys Arg           
    130                 135                 140                           

ccg gta gag cag tcg cca caa gag cca gac tcc tcc tcg ggc atc ggc        480
Pro Val Glu Gln Ser Pro Gln Glu Pro Asp Ser Ser Ser Gly Ile Gly           
145                 150                 155                 160           

aag aca ggc cag cag ccc gct aaa aag aga ctc aat ttt ggt cag act        528
Lys Thr Gly Gln Gln Pro Ala Lys Lys Arg Leu Asn Phe Gly Gln Thr           
                165                 170                 175               

ggc gac tca gag tca gtc ccc gat cca caa cct ctc gga gaa cct cca        576
Gly Asp Ser Glu Ser Val Pro Asp Pro Gln Pro Leu Gly Glu Pro Pro           
            180                 185                 190                   

gca acc ccc gct gct gtg gga cct act aca atg gct tca ggc ggt ggc        624
Ala Thr Pro Ala Ala Val Gly Pro Thr Thr Met Ala Ser Gly Gly Gly           
        195                 200                 205                       

gca cca atg gca gac aat aac gaa ggc gcc gac gga gtg ggt aat gcc        672
Ala Pro Met Ala Asp Asn Asn Glu Gly Ala Asp Gly Val Gly Asn Ala           
    210                 215                 220                           

tca gga aat tgg cat tgc gat tcc aca tgg ctg ggc gac aga gtc atc        720
Ser Gly Asn Trp His Cys Asp Ser Thr Trp Leu Gly Asp Arg Val Ile           
225                 230                 235                 240           

acc acc agc acc cgc acc tgg gcc ttg ccc acc tac aat aac cac ctc        768
Thr Thr Ser Thr Arg Thr Trp Ala Leu Pro Thr Tyr Asn Asn His Leu           
                245                 250                 255               

tac aag caa atc tcc agt gct tca acg ggg gcc agc aac gac aac cac        816
Tyr Lys Gln Ile Ser Ser Ala Ser Thr Gly Ala Ser Asn Asp Asn His           
            260                 265                 270                   

tac ttc ggc tac agc acc ccc tgg ggg tat ttt gat ttc aac aga ttc        864
Tyr Phe Gly Tyr Ser Thr Pro Trp Gly Tyr Phe Asp Phe Asn Arg Phe           
        275                 280                 285                       

cac tgc cac ttt tca cca cgt gac tgg cag cga ctc atc aac aac aat        912
His Cys His Phe Ser Pro Arg Asp Trp Gln Arg Leu Ile Asn Asn Asn           
    290                 295                 300                           

tgg gga ttc cgg ccc aag aga ctc aac ttc aaa ctc ttc aac atc caa        960
Trp Gly Phe Arg Pro Lys Arg Leu Asn Phe Lys Leu Phe Asn Ile Gln           
305                 310                 315                 320           

gtc aag gag gtc acg acg aat gat ggc gtc aca acc atc gct aat aac       1008
Val Lys Glu Val Thr Thr Asn Asp Gly Val Thr Thr Ile Ala Asn Asn           
                325                 330                 335               

ctt acc agc acg gtt caa gtc ttc tcg gac tcg gag tac cag ctt ccg       1056
Leu Thr Ser Thr Val Gln Val Phe Ser Asp Ser Glu Tyr Gln Leu Pro           
            340                 345                 350                   

tac gtc ctc ggc tct gcg cac cag ggc tgc ctc cct ccg ttc ccg gcg       1104
Tyr Val Leu Gly Ser Ala His Gln Gly Cys Leu Pro Pro Phe Pro Ala           
        355                 360                 365                       

gac gtg ttc atg att ccg caa tac ggc tac ctg acg ctc aac aat ggc       1152
Asp Val Phe Met Ile Pro Gln Tyr Gly Tyr Leu Thr Leu Asn Asn Gly           
    370                 375                 380                           

agc caa gcc gtg gga cgt tca tcc ttt tac tgc ctg gaa tat ttc cct       1200
Ser Gln Ala Val Gly Arg Ser Ser Phe Tyr Cys Leu Glu Tyr Phe Pro           
385                 390                 395                 400           

tct cag atg ctg aga acg ggc aac aac ttt acc ttc agc tac acc ttt       1248
Ser Gln Met Leu Arg Thr Gly Asn Asn Phe Thr Phe Ser Tyr Thr Phe           
                405                 410                 415               

gag gaa gtg cct ttc cac agc agc tac gcg cac agc cag agc ctg gac       1296
Glu Glu Val Pro Phe His Ser Ser Tyr Ala His Ser Gln Ser Leu Asp           
            420                 425                 430                   

cgg ctg atg aat cct ctc atc gac caa tac ctg tat tac ctg aac aga       1344
Arg Leu Met Asn Pro Leu Ile Asp Gln Tyr Leu Tyr Tyr Leu Asn Arg           
        435                 440                 445                       

act caa aat cag tcc gga agt gcc caa aac aag gac ttg ctg ttt agc       1392
Thr Gln Asn Gln Ser Gly Ser Ala Gln Asn Lys Asp Leu Leu Phe Ser           
    450                 455                 460                           

cgt ggg tct cca gct ggc atg tct gtt cag ccc aaa aac tgg cta cct       1440
Arg Gly Ser Pro Ala Gly Met Ser Val Gln Pro Lys Asn Trp Leu Pro           
465                 470                 475                 480           

gga ccc tgt tat cgg cag cag cgc gtt tct aaa aca aaa aca gac aac       1488
Gly Pro Cys Tyr Arg Gln Gln Arg Val Ser Lys Thr Lys Thr Asp Asn           
                485                 490                 495               

aac aac agc aat ttt acc tgg act ggt gct tca aaa tat aac ctc aat       1536
Asn Asn Ser Asn Phe Thr Trp Thr Gly Ala Ser Lys Tyr Asn Leu Asn           
            500                 505                 510                   

ggg cgt gaa tcc atc atc aac cct ggc act gct atg gcc tca cac aaa       1584
Gly Arg Glu Ser Ile Ile Asn Pro Gly Thr Ala Met Ala Ser His Lys           
        515                 520                 525                       

gac gac gaa gac aag ttc ttt ccc atg agc ggt gtc atg att ttt gga       1632
Asp Asp Glu Asp Lys Phe Phe Pro Met Ser Gly Val Met Ile Phe Gly           
    530                 535                 540                           

aaa gag agc gcc gga gct tca aac act gca ttg gac aat gtc atg att       1680
Lys Glu Ser Ala Gly Ala Ser Asn Thr Ala Leu Asp Asn Val Met Ile           
545                 550                 555                 560           

aca gac gaa gag gaa att aaa gcc act aac cct gtg gcc acc gaa aga       1728
Thr Asp Glu Glu Glu Ile Lys Ala Thr Asn Pro Val Ala Thr Glu Arg           
                565                 570                 575               

ttt ggg acc gtg gca gtc aat ttc cag agc agc agc aca gac cct gcg       1776
Phe Gly Thr Val Ala Val Asn Phe Gln Ser Ser Ser Thr Asp Pro Ala           
            580                 585                 590                   

acc gga gat gtg cat gct atg gga gca tta cct ggc atg gtg tgg caa       1824
Thr Gly Asp Val His Ala Met Gly Ala Leu Pro Gly Met Val Trp Gln           
        595                 600                 605                       

gat aga gac gtg tac ctg cag ggt ccc att tgg gcc aaa att cct cac       1872
Asp Arg Asp Val Tyr Leu Gln Gly Pro Ile Trp Ala Lys Ile Pro His           
    610                 615                 620                           

aca gat gga cac ttt cac ccg tct cct ctt atg ggc ggc ttt gga ctc       1920
Thr Asp Gly His Phe His Pro Ser Pro Leu Met Gly Gly Phe Gly Leu           
625                 630                 635                 640           

aag aac ccg cct cct cag atc ctc atc aaa aac acg cct gtt cct gcg       1968
Lys Asn Pro Pro Pro Gln Ile Leu Ile Lys Asn Thr Pro Val Pro Ala           
                645                 650                 655               

aat cct ccg gcg gag ttt tca gct aca aag ttt gct tca ttc atc acc       2016
Asn Pro Pro Ala Glu Phe Ser Ala Thr Lys Phe Ala Ser Phe Ile Thr           
            660                 665                 670                   

caa tac tcc aca gga caa gtg agt gtg gaa att gaa tgg gag ctg cag       2064
Gln Tyr Ser Thr Gly Gln Val Ser Val Glu Ile Glu Trp Glu Leu Gln           
        675                 680                 685                       

aaa gaa aac agc aag cgc tgg aat ccc gaa gtg cag tac aca tcc aat       2112
Lys Glu Asn Ser Lys Arg Trp Asn Pro Glu Val Gln Tyr Thr Ser Asn           
    690                 695                 700                           

tat gca aaa tct gcc aac gtt gat ttt act gtg gac aac aat gga ctt       2160
Tyr Ala Lys Ser Ala Asn Val Asp Phe Thr Val Asp Asn Asn Gly Leu           
705                 710                 715                 720           

tat act gag cct cgc ccc att ggc acc cgt tac ctt acc cgt ccc ctg       2208
Tyr Thr Glu Pro Arg Pro Ile Gly Thr Arg Tyr Leu Thr Arg Pro Leu           
                725                 730                 735               


<210>  21
<211>  736
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Synthetic Construct

<400>  21

Met Ala Ala Asp Gly Tyr Leu Pro Asp Trp Leu Glu Asp Asn Leu Ser 
1               5                   10                  15      


Glu Gly Ile Arg Glu Trp Trp Asp Leu Lys Pro Gly Ala Pro Lys Pro 
            20                  25                  30          


Lys Ala Asn Gln Gln Lys Gln Asp Asp Gly Arg Gly Leu Val Leu Pro 
        35                  40                  45              


Gly Tyr Lys Tyr Leu Gly Pro Phe Asn Gly Leu Asp Lys Gly Glu Pro 
    50                  55                  60                  


Val Asn Ala Ala Asp Ala Ala Ala Leu Glu His Asp Lys Ala Tyr Asp 
65                  70                  75                  80  


Gln Gln Leu Lys Ala Gly Asp Asn Pro Tyr Leu Arg Tyr Asn His Ala 
                85                  90                  95      


Asp Ala Glu Phe Gln Glu Arg Leu Gln Glu Asp Thr Ser Phe Gly Gly 
            100                 105                 110         


Asn Leu Gly Arg Ala Val Phe Gln Ala Lys Lys Arg Val Leu Glu Pro 
        115                 120                 125             


Leu Gly Leu Val Glu Glu Gly Ala Lys Thr Ala Pro Gly Lys Lys Arg 
    130                 135                 140                 


Pro Val Glu Gln Ser Pro Gln Glu Pro Asp Ser Ser Ser Gly Ile Gly 
145                 150                 155                 160 


Lys Thr Gly Gln Gln Pro Ala Lys Lys Arg Leu Asn Phe Gly Gln Thr 
                165                 170                 175     


Gly Asp Ser Glu Ser Val Pro Asp Pro Gln Pro Leu Gly Glu Pro Pro 
            180                 185                 190         


Ala Thr Pro Ala Ala Val Gly Pro Thr Thr Met Ala Ser Gly Gly Gly 
        195                 200                 205             


Ala Pro Met Ala Asp Asn Asn Glu Gly Ala Asp Gly Val Gly Asn Ala 
    210                 215                 220                 


Ser Gly Asn Trp His Cys Asp Ser Thr Trp Leu Gly Asp Arg Val Ile 
225                 230                 235                 240 


Thr Thr Ser Thr Arg Thr Trp Ala Leu Pro Thr Tyr Asn Asn His Leu 
                245                 250                 255     


Tyr Lys Gln Ile Ser Ser Ala Ser Thr Gly Ala Ser Asn Asp Asn His 
            260                 265                 270         


Tyr Phe Gly Tyr Ser Thr Pro Trp Gly Tyr Phe Asp Phe Asn Arg Phe 
        275                 280                 285             


His Cys His Phe Ser Pro Arg Asp Trp Gln Arg Leu Ile Asn Asn Asn 
    290                 295                 300                 


Trp Gly Phe Arg Pro Lys Arg Leu Asn Phe Lys Leu Phe Asn Ile Gln 
305                 310                 315                 320 


Val Lys Glu Val Thr Thr Asn Asp Gly Val Thr Thr Ile Ala Asn Asn 
                325                 330                 335     


Leu Thr Ser Thr Val Gln Val Phe Ser Asp Ser Glu Tyr Gln Leu Pro 
            340                 345                 350         


Tyr Val Leu Gly Ser Ala His Gln Gly Cys Leu Pro Pro Phe Pro Ala 
        355                 360                 365             


Asp Val Phe Met Ile Pro Gln Tyr Gly Tyr Leu Thr Leu Asn Asn Gly 
    370                 375                 380                 


Ser Gln Ala Val Gly Arg Ser Ser Phe Tyr Cys Leu Glu Tyr Phe Pro 
385                 390                 395                 400 


Ser Gln Met Leu Arg Thr Gly Asn Asn Phe Thr Phe Ser Tyr Thr Phe 
                405                 410                 415     


Glu Glu Val Pro Phe His Ser Ser Tyr Ala His Ser Gln Ser Leu Asp 
            420                 425                 430         


Arg Leu Met Asn Pro Leu Ile Asp Gln Tyr Leu Tyr Tyr Leu Asn Arg 
        435                 440                 445             


Thr Gln Asn Gln Ser Gly Ser Ala Gln Asn Lys Asp Leu Leu Phe Ser 
    450                 455                 460                 


Arg Gly Ser Pro Ala Gly Met Ser Val Gln Pro Lys Asn Trp Leu Pro 
465                 470                 475                 480 


Gly Pro Cys Tyr Arg Gln Gln Arg Val Ser Lys Thr Lys Thr Asp Asn 
                485                 490                 495     


Asn Asn Ser Asn Phe Thr Trp Thr Gly Ala Ser Lys Tyr Asn Leu Asn 
            500                 505                 510         


Gly Arg Glu Ser Ile Ile Asn Pro Gly Thr Ala Met Ala Ser His Lys 
        515                 520                 525             


Asp Asp Glu Asp Lys Phe Phe Pro Met Ser Gly Val Met Ile Phe Gly 
    530                 535                 540                 


Lys Glu Ser Ala Gly Ala Ser Asn Thr Ala Leu Asp Asn Val Met Ile 
545                 550                 555                 560 


Thr Asp Glu Glu Glu Ile Lys Ala Thr Asn Pro Val Ala Thr Glu Arg 
                565                 570                 575     


Phe Gly Thr Val Ala Val Asn Phe Gln Ser Ser Ser Thr Asp Pro Ala 
            580                 585                 590         


Thr Gly Asp Val His Ala Met Gly Ala Leu Pro Gly Met Val Trp Gln 
        595                 600                 605             


Asp Arg Asp Val Tyr Leu Gln Gly Pro Ile Trp Ala Lys Ile Pro His 
    610                 615                 620                 


Thr Asp Gly His Phe His Pro Ser Pro Leu Met Gly Gly Phe Gly Leu 
625                 630                 635                 640 


Lys Asn Pro Pro Pro Gln Ile Leu Ile Lys Asn Thr Pro Val Pro Ala 
                645                 650                 655     


Asn Pro Pro Ala Glu Phe Ser Ala Thr Lys Phe Ala Ser Phe Ile Thr 
            660                 665                 670         


Gln Tyr Ser Thr Gly Gln Val Ser Val Glu Ile Glu Trp Glu Leu Gln 
        675                 680                 685             


Lys Glu Asn Ser Lys Arg Trp Asn Pro Glu Val Gln Tyr Thr Ser Asn 
    690                 695                 700                 


Tyr Ala Lys Ser Ala Asn Val Asp Phe Thr Val Asp Asn Asn Gly Leu 
705                 710                 715                 720 


Tyr Thr Glu Pro Arg Pro Ile Gly Thr Arg Tyr Leu Thr Arg Pro Leu 
                725                 730                 735     


<210>  22
<211>  2172
<212>  DNA
<213>  Artificial sequence

<220>
<223>  AAV5 capsid VP1


<220>
<221>  CDS
<222>  (1)..(2172)

<400>  22
atg tct ttt gtt gat cac cct cca gat tgg ttg gaa gaa gtt ggt gaa         48
Met Ser Phe Val Asp His Pro Pro Asp Trp Leu Glu Glu Val Gly Glu           
1               5                   10                  15                

ggt ctt cgc gag ttt ttg ggc ctt gaa gcg ggc cca ccg aaa cca aaa         96
Gly Leu Arg Glu Phe Leu Gly Leu Glu Ala Gly Pro Pro Lys Pro Lys           
            20                  25                  30                    

ccc aat cag cag cat caa gat caa gcc cgt ggt ctt gtg ctg cct ggt        144
Pro Asn Gln Gln His Gln Asp Gln Ala Arg Gly Leu Val Leu Pro Gly           
        35                  40                  45                        

tat aac tat ctc gga ccc gga aac ggt ctc gat cga gga gag cct gtc        192
Tyr Asn Tyr Leu Gly Pro Gly Asn Gly Leu Asp Arg Gly Glu Pro Val           
    50                  55                  60                            

aac agg gca gac gag gtc gcg cga gag cac gac atc tcg tac aac gag        240
Asn Arg Ala Asp Glu Val Ala Arg Glu His Asp Ile Ser Tyr Asn Glu           
65                  70                  75                  80            

cag ctt gag gcg gga gac aac ccc tac ctc aag tac aac cac gcg gac        288
Gln Leu Glu Ala Gly Asp Asn Pro Tyr Leu Lys Tyr Asn His Ala Asp           
                85                  90                  95                

gcc gag ttt cag gag aag ctc gcc gac gac aca tcc ttc ggg gga aac        336
Ala Glu Phe Gln Glu Lys Leu Ala Asp Asp Thr Ser Phe Gly Gly Asn           
            100                 105                 110                   

ctc gga aag gca gtc ttt cag gcc aag aaa agg gtt ctc gaa cct ttt        384
Leu Gly Lys Ala Val Phe Gln Ala Lys Lys Arg Val Leu Glu Pro Phe           
        115                 120                 125                       

ggc ctg gtt gaa gag ggt gct aag acg gcc cct acc gga aag cgg ata        432
Gly Leu Val Glu Glu Gly Ala Lys Thr Ala Pro Thr Gly Lys Arg Ile           
    130                 135                 140                           

gac gac cac ttt cca aaa aga aag aag gcc cgg acc gaa gag gac tcc        480
Asp Asp His Phe Pro Lys Arg Lys Lys Ala Arg Thr Glu Glu Asp Ser           
145                 150                 155                 160           

aag cct tcc acc tcg tca gac gcc gaa gct gga ccc agc gga tcc cag        528
Lys Pro Ser Thr Ser Ser Asp Ala Glu Ala Gly Pro Ser Gly Ser Gln           
                165                 170                 175               

cag ctg caa atc cca gcc caa cca gcc tca agt ttg gga gct gat aca        576
Gln Leu Gln Ile Pro Ala Gln Pro Ala Ser Ser Leu Gly Ala Asp Thr           
            180                 185                 190                   

atg tct gcg gga ggt ggc ggc cca ttg ggc gac aat aac caa ggt gcc        624
Met Ser Ala Gly Gly Gly Gly Pro Leu Gly Asp Asn Asn Gln Gly Ala           
        195                 200                 205                       

gat gga gtg ggc aat gcc tcg gga gat tgg cat tgc gat tcc acg tgg        672
Asp Gly Val Gly Asn Ala Ser Gly Asp Trp His Cys Asp Ser Thr Trp           
    210                 215                 220                           

atg ggg gac aga gtc gtc acc aag tcc acc cga acc tgg gtg ctg ccc        720
Met Gly Asp Arg Val Val Thr Lys Ser Thr Arg Thr Trp Val Leu Pro           
225                 230                 235                 240           

agc tac aac aac cac cag tac cga gag atc aaa agc ggc tcc gtc gac        768
Ser Tyr Asn Asn His Gln Tyr Arg Glu Ile Lys Ser Gly Ser Val Asp           
                245                 250                 255               

gga agc aac gcc aac gcc tac ttt gga tac agc acc ccc tgg ggg tac        816
Gly Ser Asn Ala Asn Ala Tyr Phe Gly Tyr Ser Thr Pro Trp Gly Tyr           
            260                 265                 270                   

ttt gac ttt aac cgc ttc cac agc cac tgg agc ccc cga gac tgg caa        864
Phe Asp Phe Asn Arg Phe His Ser His Trp Ser Pro Arg Asp Trp Gln           
        275                 280                 285                       

aga ctc atc aac aac tac tgg ggc ttc aga ccc cgg tcc ctc aga gtc        912
Arg Leu Ile Asn Asn Tyr Trp Gly Phe Arg Pro Arg Ser Leu Arg Val           
    290                 295                 300                           

aaa atc ttc aac att caa gtc aaa gag gtc acg gtg cag gac tcc acc        960
Lys Ile Phe Asn Ile Gln Val Lys Glu Val Thr Val Gln Asp Ser Thr           
305                 310                 315                 320           

acc acc atc gcc aac aac ctc acc tcc acc gtc caa gtg ttt acg gac       1008
Thr Thr Ile Ala Asn Asn Leu Thr Ser Thr Val Gln Val Phe Thr Asp           
                325                 330                 335               

gac gac tac cag ctg ccc tac gtc gtc ggc aac ggg acc gag gga tgc       1056
Asp Asp Tyr Gln Leu Pro Tyr Val Val Gly Asn Gly Thr Glu Gly Cys           
            340                 345                 350                   

ctg ccg gcc ttc cct ccg cag gtc ttt acg ctg ccg cag tac ggt tac       1104
Leu Pro Ala Phe Pro Pro Gln Val Phe Thr Leu Pro Gln Tyr Gly Tyr           
        355                 360                 365                       

gcg acg ctg aac cgc gac aac aca gaa aat ccc acc gag agg agc agc       1152
Ala Thr Leu Asn Arg Asp Asn Thr Glu Asn Pro Thr Glu Arg Ser Ser           
    370                 375                 380                           

ttc ttc tgc cta gag tac ttt ccc agc aag atg ctg aga acg ggc aac       1200
Phe Phe Cys Leu Glu Tyr Phe Pro Ser Lys Met Leu Arg Thr Gly Asn           
385                 390                 395                 400           

aac ttt gag ttt acc tac aac ttt gag gag gtg ccc ttc cac tcc agc       1248
Asn Phe Glu Phe Thr Tyr Asn Phe Glu Glu Val Pro Phe His Ser Ser           
                405                 410                 415               

ttc gct ccc agt cag aac ctc ttc aag ctg gcc aac ccg ctg gtg gac       1296
Phe Ala Pro Ser Gln Asn Leu Phe Lys Leu Ala Asn Pro Leu Val Asp           
            420                 425                 430                   

cag tac ttg tac cgc ttc gtg agc aca aat aac act ggc gga gtc cag       1344
Gln Tyr Leu Tyr Arg Phe Val Ser Thr Asn Asn Thr Gly Gly Val Gln           
        435                 440                 445                       

ttc aac aag aac ctg gcc ggg aga tac gcc aac acc tac aaa aac tgg       1392
Phe Asn Lys Asn Leu Ala Gly Arg Tyr Ala Asn Thr Tyr Lys Asn Trp           
    450                 455                 460                           

ttc ccg ggg ccc atg ggc cga acc cag ggc tgg aac ctg ggc tcc ggg       1440
Phe Pro Gly Pro Met Gly Arg Thr Gln Gly Trp Asn Leu Gly Ser Gly           
465                 470                 475                 480           

gtc aac cgc gcc agt gtc agc gcc ttc gcc acg acc aat agg atg gag       1488
Val Asn Arg Ala Ser Val Ser Ala Phe Ala Thr Thr Asn Arg Met Glu           
                485                 490                 495               

ctc gag ggc gcg agt tac cag gtg ccc ccg cag ccg aac ggc atg acc       1536
Leu Glu Gly Ala Ser Tyr Gln Val Pro Pro Gln Pro Asn Gly Met Thr           
            500                 505                 510                   

aac aac ctc cag ggc agc aac acc tat gcc ctg gag aac act atg atc       1584
Asn Asn Leu Gln Gly Ser Asn Thr Tyr Ala Leu Glu Asn Thr Met Ile           
        515                 520                 525                       

ttc aac agc cag ccg gcg aac ccg ggc acc acc gcc acg tac ctc gag       1632
Phe Asn Ser Gln Pro Ala Asn Pro Gly Thr Thr Ala Thr Tyr Leu Glu           
    530                 535                 540                           

ggc aac atg ctc atc acc agc gag agc gag acg cag ccg gtg aac cgc       1680
Gly Asn Met Leu Ile Thr Ser Glu Ser Glu Thr Gln Pro Val Asn Arg           
545                 550                 555                 560           

gtg gcg tac aac gtc ggc ggg cag atg gcc acc aac aac cag agc tcc       1728
Val Ala Tyr Asn Val Gly Gly Gln Met Ala Thr Asn Asn Gln Ser Ser           
                565                 570                 575               

acc act gcc ccc gcg acc ggc acg tac aac ctc cag gaa atc gtg ccc       1776
Thr Thr Ala Pro Ala Thr Gly Thr Tyr Asn Leu Gln Glu Ile Val Pro           
            580                 585                 590                   

ggc agc gtg tgg atg gag agg gac gtg tac ctc caa gga ccc atc tgg       1824
Gly Ser Val Trp Met Glu Arg Asp Val Tyr Leu Gln Gly Pro Ile Trp           
        595                 600                 605                       

gcc aag atc cca gag acg ggg gcg cac ttt cac ccc tct ccg gcc atg       1872
Ala Lys Ile Pro Glu Thr Gly Ala His Phe His Pro Ser Pro Ala Met           
    610                 615                 620                           

ggc gga ttc gga ctc aaa cac cca ccg ccc atg atg ctc atc aag aac       1920
Gly Gly Phe Gly Leu Lys His Pro Pro Pro Met Met Leu Ile Lys Asn           
625                 630                 635                 640           

acg cct gtg ccc gga aat atc acc agc ttc tcg gac gtg ccc gtc agc       1968
Thr Pro Val Pro Gly Asn Ile Thr Ser Phe Ser Asp Val Pro Val Ser           
                645                 650                 655               

agc ttc atc acc cag tac agc acc ggg cag gtc acc gtg gag atg gag       2016
Ser Phe Ile Thr Gln Tyr Ser Thr Gly Gln Val Thr Val Glu Met Glu           
            660                 665                 670                   

tgg gag ctc aag aag gaa aac tcc aag agg tgg aac cca gag atc cag       2064
Trp Glu Leu Lys Lys Glu Asn Ser Lys Arg Trp Asn Pro Glu Ile Gln           
        675                 680                 685                       

tac aca aac aac tac aac gac ccc cag ttt gtg gac ttt gcc ccg gac       2112
Tyr Thr Asn Asn Tyr Asn Asp Pro Gln Phe Val Asp Phe Ala Pro Asp           
    690                 695                 700                           

agc acc ggg gaa tac aga acc acc aga cct atc gga acc cga tac ctt       2160
Ser Thr Gly Glu Tyr Arg Thr Thr Arg Pro Ile Gly Thr Arg Tyr Leu           
705                 710                 715                 720           

acc cga ccc ctt                                                       2172
Thr Arg Pro Leu                                                           
                                                                          


<210>  23
<211>  724
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Synthetic Construct

<400>  23

Met Ser Phe Val Asp His Pro Pro Asp Trp Leu Glu Glu Val Gly Glu 
1               5                   10                  15      


Gly Leu Arg Glu Phe Leu Gly Leu Glu Ala Gly Pro Pro Lys Pro Lys 
            20                  25                  30          


Pro Asn Gln Gln His Gln Asp Gln Ala Arg Gly Leu Val Leu Pro Gly 
        35                  40                  45              


Tyr Asn Tyr Leu Gly Pro Gly Asn Gly Leu Asp Arg Gly Glu Pro Val 
    50                  55                  60                  


Asn Arg Ala Asp Glu Val Ala Arg Glu His Asp Ile Ser Tyr Asn Glu 
65                  70                  75                  80  


Gln Leu Glu Ala Gly Asp Asn Pro Tyr Leu Lys Tyr Asn His Ala Asp 
                85                  90                  95      


Ala Glu Phe Gln Glu Lys Leu Ala Asp Asp Thr Ser Phe Gly Gly Asn 
            100                 105                 110         


Leu Gly Lys Ala Val Phe Gln Ala Lys Lys Arg Val Leu Glu Pro Phe 
        115                 120                 125             


Gly Leu Val Glu Glu Gly Ala Lys Thr Ala Pro Thr Gly Lys Arg Ile 
    130                 135                 140                 


Asp Asp His Phe Pro Lys Arg Lys Lys Ala Arg Thr Glu Glu Asp Ser 
145                 150                 155                 160 


Lys Pro Ser Thr Ser Ser Asp Ala Glu Ala Gly Pro Ser Gly Ser Gln 
                165                 170                 175     


Gln Leu Gln Ile Pro Ala Gln Pro Ala Ser Ser Leu Gly Ala Asp Thr 
            180                 185                 190         


Met Ser Ala Gly Gly Gly Gly Pro Leu Gly Asp Asn Asn Gln Gly Ala 
        195                 200                 205             


Asp Gly Val Gly Asn Ala Ser Gly Asp Trp His Cys Asp Ser Thr Trp 
    210                 215                 220                 


Met Gly Asp Arg Val Val Thr Lys Ser Thr Arg Thr Trp Val Leu Pro 
225                 230                 235                 240 


Ser Tyr Asn Asn His Gln Tyr Arg Glu Ile Lys Ser Gly Ser Val Asp 
                245                 250                 255     


Gly Ser Asn Ala Asn Ala Tyr Phe Gly Tyr Ser Thr Pro Trp Gly Tyr 
            260                 265                 270         


Phe Asp Phe Asn Arg Phe His Ser His Trp Ser Pro Arg Asp Trp Gln 
        275                 280                 285             


Arg Leu Ile Asn Asn Tyr Trp Gly Phe Arg Pro Arg Ser Leu Arg Val 
    290                 295                 300                 


Lys Ile Phe Asn Ile Gln Val Lys Glu Val Thr Val Gln Asp Ser Thr 
305                 310                 315                 320 


Thr Thr Ile Ala Asn Asn Leu Thr Ser Thr Val Gln Val Phe Thr Asp 
                325                 330                 335     


Asp Asp Tyr Gln Leu Pro Tyr Val Val Gly Asn Gly Thr Glu Gly Cys 
            340                 345                 350         


Leu Pro Ala Phe Pro Pro Gln Val Phe Thr Leu Pro Gln Tyr Gly Tyr 
        355                 360                 365             


Ala Thr Leu Asn Arg Asp Asn Thr Glu Asn Pro Thr Glu Arg Ser Ser 
    370                 375                 380                 


Phe Phe Cys Leu Glu Tyr Phe Pro Ser Lys Met Leu Arg Thr Gly Asn 
385                 390                 395                 400 


Asn Phe Glu Phe Thr Tyr Asn Phe Glu Glu Val Pro Phe His Ser Ser 
                405                 410                 415     


Phe Ala Pro Ser Gln Asn Leu Phe Lys Leu Ala Asn Pro Leu Val Asp 
            420                 425                 430         


Gln Tyr Leu Tyr Arg Phe Val Ser Thr Asn Asn Thr Gly Gly Val Gln 
        435                 440                 445             


Phe Asn Lys Asn Leu Ala Gly Arg Tyr Ala Asn Thr Tyr Lys Asn Trp 
    450                 455                 460                 


Phe Pro Gly Pro Met Gly Arg Thr Gln Gly Trp Asn Leu Gly Ser Gly 
465                 470                 475                 480 


Val Asn Arg Ala Ser Val Ser Ala Phe Ala Thr Thr Asn Arg Met Glu 
                485                 490                 495     


Leu Glu Gly Ala Ser Tyr Gln Val Pro Pro Gln Pro Asn Gly Met Thr 
            500                 505                 510         


Asn Asn Leu Gln Gly Ser Asn Thr Tyr Ala Leu Glu Asn Thr Met Ile 
        515                 520                 525             


Phe Asn Ser Gln Pro Ala Asn Pro Gly Thr Thr Ala Thr Tyr Leu Glu 
    530                 535                 540                 


Gly Asn Met Leu Ile Thr Ser Glu Ser Glu Thr Gln Pro Val Asn Arg 
545                 550                 555                 560 


Val Ala Tyr Asn Val Gly Gly Gln Met Ala Thr Asn Asn Gln Ser Ser 
                565                 570                 575     


Thr Thr Ala Pro Ala Thr Gly Thr Tyr Asn Leu Gln Glu Ile Val Pro 
            580                 585                 590         


Gly Ser Val Trp Met Glu Arg Asp Val Tyr Leu Gln Gly Pro Ile Trp 
        595                 600                 605             


Ala Lys Ile Pro Glu Thr Gly Ala His Phe His Pro Ser Pro Ala Met 
    610                 615                 620                 


Gly Gly Phe Gly Leu Lys His Pro Pro Pro Met Met Leu Ile Lys Asn 
625                 630                 635                 640 


Thr Pro Val Pro Gly Asn Ile Thr Ser Phe Ser Asp Val Pro Val Ser 
                645                 650                 655     


Ser Phe Ile Thr Gln Tyr Ser Thr Gly Gln Val Thr Val Glu Met Glu 
            660                 665                 670         


Trp Glu Leu Lys Lys Glu Asn Ser Lys Arg Trp Asn Pro Glu Ile Gln 
        675                 680                 685             


Tyr Thr Asn Asn Tyr Asn Asp Pro Gln Phe Val Asp Phe Ala Pro Asp 
    690                 695                 700                 


Ser Thr Gly Glu Tyr Arg Thr Thr Arg Pro Ile Gly Thr Arg Tyr Leu 
705                 710                 715                 720 


Thr Arg Pro Leu 
                


<210>  24
<211>  736
<212>  PRT
<213>  Artificial sequence

<220>
<223>  AAV3B VP1 Capsid

<400>  24

Met Ala Ala Asp Gly Tyr Leu Pro Asp Trp Leu Glu Asp Asn Leu Ser 
1               5                   10                  15      


Glu Gly Ile Arg Glu Trp Trp Ala Leu Lys Pro Gly Val Pro Gln Pro 
            20                  25                  30          


Lys Ala Asn Gln Gln His Gln Asp Asn Arg Arg Gly Leu Val Leu Pro 
        35                  40                  45              


Gly Tyr Lys Tyr Leu Gly Pro Gly Asn Gly Leu Asp Lys Gly Glu Pro 
    50                  55                  60                  


Val Asn Glu Ala Asp Ala Ala Ala Leu Glu His Asp Lys Ala Tyr Asp 
65                  70                  75                  80  


Gln Gln Leu Lys Ala Gly Asp Asn Pro Tyr Leu Lys Tyr Asn His Ala 
                85                  90                  95      


Asp Ala Glu Phe Gln Glu Arg Leu Gln Glu Asp Thr Ser Phe Gly Gly 
            100                 105                 110         


Asn Leu Gly Arg Ala Val Phe Gln Ala Lys Lys Arg Ile Leu Glu Pro 
        115                 120                 125             


Leu Gly Leu Val Glu Glu Ala Ala Lys Thr Ala Pro Gly Lys Lys Arg 
    130                 135                 140                 


Pro Val Asp Gln Ser Pro Gln Glu Pro Asp Ser Ser Ser Gly Val Gly 
145                 150                 155                 160 


Lys Ser Gly Lys Gln Pro Ala Arg Lys Arg Leu Asn Phe Gly Gln Thr 
                165                 170                 175     


Gly Asp Ser Glu Ser Val Pro Asp Pro Gln Pro Leu Gly Glu Pro Pro 
            180                 185                 190         


Ala Ala Pro Thr Ser Leu Gly Ser Asn Thr Met Ala Ser Gly Gly Gly 
        195                 200                 205             


Ala Pro Met Ala Asp Asn Asn Glu Gly Ala Asp Gly Val Gly Asn Ser 
    210                 215                 220                 


Ser Gly Asn Trp His Cys Asp Ser Gln Trp Leu Gly Asp Arg Val Ile 
225                 230                 235                 240 


Thr Thr Ser Thr Arg Thr Trp Ala Leu Pro Thr Tyr Asn Asn His Leu 
                245                 250                 255     


Tyr Lys Gln Ile Ser Ser Gln Ser Gly Ala Ser Asn Asp Asn His Tyr 
            260                 265                 270         


Phe Gly Tyr Ser Thr Pro Trp Gly Tyr Phe Asp Phe Asn Arg Phe His 
        275                 280                 285             


Cys His Phe Ser Pro Arg Asp Trp Gln Arg Leu Ile Asn Asn Asn Trp 
    290                 295                 300                 


Gly Phe Arg Pro Lys Lys Leu Ser Phe Lys Leu Phe Asn Ile Gln Val 
305                 310                 315                 320 


Lys Glu Val Thr Gln Asn Asp Gly Thr Thr Thr Ile Ala Asn Asn Leu 
                325                 330                 335     


Thr Ser Thr Val Gln Val Phe Thr Asp Ser Glu Tyr Gln Leu Pro Tyr 
            340                 345                 350         


Val Leu Gly Ser Ala His Gln Gly Cys Leu Pro Pro Phe Pro Ala Asp 
        355                 360                 365             


Val Phe Met Val Pro Gln Tyr Gly Tyr Leu Thr Leu Asn Asn Gly Ser 
    370                 375                 380                 


Gln Ala Val Gly Arg Ser Ser Phe Tyr Cys Leu Glu Tyr Phe Pro Ser 
385                 390                 395                 400 


Gln Met Leu Arg Thr Gly Asn Asn Phe Gln Phe Ser Tyr Thr Phe Glu 
                405                 410                 415     


Asp Val Pro Phe His Ser Ser Tyr Ala His Ser Gln Ser Leu Asp Arg 
            420                 425                 430         


Leu Met Asn Pro Leu Ile Asp Gln Tyr Leu Tyr Tyr Leu Asn Arg Thr 
        435                 440                 445             


Gln Gly Thr Thr Ser Gly Thr Thr Asn Gln Ser Arg Leu Leu Phe Ser 
    450                 455                 460                 


Gln Ala Gly Pro Gln Ser Met Ser Leu Gln Ala Arg Asn Trp Leu Pro 
465                 470                 475                 480 


Gly Pro Cys Tyr Arg Gln Gln Arg Leu Ser Lys Thr Ala Asn Asp Asn 
                485                 490                 495     


Asn Asn Ser Asn Phe Pro Trp Thr Ala Ala Ser Lys Tyr His Leu Asn 
            500                 505                 510         


Gly Arg Asp Ser Leu Val Asn Pro Gly Pro Ala Met Ala Ser His Lys 
        515                 520                 525             


Asp Asp Glu Glu Lys Phe Phe Pro Met His Gly Asn Leu Ile Phe Gly 
    530                 535                 540                 


Lys Glu Gly Thr Thr Ala Ser Asn Ala Glu Leu Asp Asn Val Met Ile 
545                 550                 555                 560 


Thr Asp Glu Glu Glu Ile Arg Thr Thr Asn Pro Val Ala Thr Glu Gln 
                565                 570                 575     


Tyr Gly Thr Val Ala Asn Asn Leu Gln Ser Ser Asn Thr Ala Pro Thr 
            580                 585                 590         


Thr Arg Thr Val Asn Asp Gln Gly Ala Leu Pro Gly Met Val Trp Gln 
        595                 600                 605             


Asp Arg Asp Val Tyr Leu Gln Gly Pro Ile Trp Ala Lys Ile Pro His 
    610                 615                 620                 


Thr Asp Gly His Phe His Pro Ser Pro Leu Met Gly Gly Phe Gly Leu 
625                 630                 635                 640 


Lys His Pro Pro Pro Gln Ile Met Ile Lys Asn Thr Pro Val Pro Ala 
                645                 650                 655     


Asn Pro Pro Thr Thr Phe Ser Pro Ala Lys Phe Ala Ser Phe Ile Thr 
            660                 665                 670         


Gln Tyr Ser Thr Gly Gln Val Ser Val Glu Ile Glu Trp Glu Leu Gln 
        675                 680                 685             


Lys Glu Asn Ser Lys Arg Trp Asn Pro Glu Ile Gln Tyr Thr Ser Asn 
    690                 695                 700                 


Tyr Asn Lys Ser Val Asn Val Asp Phe Thr Val Asp Thr Asn Gly Val 
705                 710                 715                 720 


Tyr Ser Glu Pro Arg Pro Ile Gly Thr Arg Tyr Leu Thr Arg Asn Leu 
                725                 730                 735     


<210>  25
<211>  1227
<212>  DNA
<213>  Artificial sequence

<220>
<223>  UbC promoter

<400>  25
ggcctccgcg ccgggttttg gcgcctcccg cgggcgcccc cctcctcacg gcgagcgctg       60

ccacgtcaga cgaagggcgc aggagcgtcc tgatccttcc gcccggacgc tcaggacagc      120

ggcccgctgc tcataagact cggccttaga accccagtat cagcagaagg acattttagg      180

acgggacttg ggtgactcta gggcactggt tttctttcca gagagcggaa caggcgagga      240

aaagtagtcc cttctcggcg attctgcgga gggatctccg tggggcggtg aacgccgatg      300

attatataag gacgcgccgg gtgtggcaca gctagttccg tcgcagccgg gatttgggtc      360

gcggttcttg tttgtggatc gctgtgatcg tcacttggtg agtagcgggc tgctgggctg      420

gccggggctt tcgtggccgc cgggccgctc ggtgggacgg aagcgtgtgg agagaccgcc      480

aagggctgta gtctgggtcc gcgagcaagg ttgccctgaa ctgggggttg gggggagcgc      540

agcaaaatgg cggctgttcc cgagtcttga atggaagacg cttgtgaggc gggctgtgag      600

gtcgttgaaa caaggtgggg ggcatggtgg gcggcaagaa cccaaggtct tgaggccttc      660

gctaatgcgg gaaagctctt attcgggtga gatgggctgg ggcaccatct ggggaccctg      720

acgtgaagtt tgtcactgac tggagaactc ggtttgtcgt ctgttgcggg ggcggcagtt      780

atgcggtgcc gttgggcagt gcacccgtac ctttgggagc gcgcgccctc gtcgtgtcgt      840

gacgtcaccc gttctgttgg cttataatgc agggtggggc cacctgccgg taggtgtgcg      900

gtaggctttt ctccgtcgca ggacgcaggg ttcgggccta gggtaggctc tcctgaatcg      960

acaggcgccg gacctctggt gaggggaggg ataagtgagg cgtcagtttc tttggtcggt     1020

tttatgtacc tatcttctta agtagctgaa gctccggttt tgaactatgc gctcggggtt     1080

ggcgagtgtg ttttgtgaag ttttttaggc accttttgaa atgtaatcat ttgggtcaat     1140

atgtaatttt cagtgttaga ctagtaaatt gtccgctaaa ttctggccgt ttttggcttt     1200

tttgttagac gaagctttat tgcggta                                         1227


<210>  26
<211>  232
<212>  DNA
<213>  Artificial sequence

<220>
<223>  SV40 late polyA

<400>  26
cgagcagaca tgataagata cattgatgag tttggacaaa ccacaactag aatgcagtga       60

aaaaaatgct ttatttgtga aatttgtgat gctattgctt tatttgtaac cattataagc      120

tgcaataaac aagttaacaa caacaattgc attcatttta tgtttcaggt tcagggggag      180

atgtgggagg ttttttaaag caagtaaaac ctctacaaat gtggtaaaat cg              232


<210>  27
<211>  1184
<212>  DNA
<213>  Artificial sequence

<220>
<223>  EF-1a promoter

<400>  27
cgtgaggctc cggtgcccgt cagtgggcag agcgcacatc gcccacagtc cccgagaagt       60

tggggggagg ggtcggcaat tgaaccggtg cctagagaag gtggcgcggg gtaaactggg      120

aaagtgatgt cgtgtactgg ctccgccttt ttcccgaggg tgggggagaa ccgtatataa      180

gtgcagtagt cgccgtgaac gttctttttc gcaacgggtt tgccgccaga acacaggtaa      240

gtgccgtgtg tggttcccgc gggcctggcc tctttacggg ttatggccct tgcgtgcctt      300

gaattacttc cacctggctg cagtacgtga ttcttgatcc cgagcttcgg gttggaagtg      360

ggtgggagag ttcgaggcct tgcgcttaag gagccccttc gcctcgtgct tgagttgagg      420

cctggcctgg gcgctggggc cgccgcgtgc gaatctggtg gcaccttcgc gcctgtctcg      480

ctgctttcga taagtctcta gccatttaaa atttttgatg acctgctgcg acgctttttt      540

tctggcaaga tagtcttgta aatgcgggcc aagatctgca cactggtatt tcggtttttg      600

gggccgcggg cggcgacggg gcccgtgcgt cccagcgcac atgttcggcg aggcggggcc      660

tgcgagcgcg gccaccgaga atcggacggg ggtagtctca agctggccgg cctgctctgg      720

tgcctggcct cgcgccgccg tgtatcgccc cgccctgggc ggcaaggctg gcccggtcgg      780

caccagttgc gtgagcggaa agatggccgc ttcccggccc tgctgcaggg agctcaaaat      840

ggaggacgcg gcgctcggga gagcgggcgg gtgagtcacc cacacaaagg aaaagggcct      900

ttccgtcctc agccgtcgct tcatgtgact ccacggagta ccgggcgccg tccaggcacc      960

tcgattagtt ctcgagcttt tggagtacgt cgtctttagg ttggggggag gggttttatg     1020

cgatggagtt tccccacact gagtgggtgg agactgaagt taggccagct tggcacttga     1080

tgtaattctc cttggaattt gccctttttg agtttggatc ttggttcatt ctcaagcctc     1140

agacagtggt tcaaagtttt tttcttccat ttcaggtgtc gtga                      1184


