                         SEQUENCE LISTING

<110>  UNIVERSITY OF PITTSBURGH - OF THE COMMONWEALTH SYSTEM OF 
       HIGHER EDUCATION
 
<120>  RCOM PROTEIN BASED CARBON MONOXIDE SCAVENGERS AND PREPARATIONS 
       FOR THE TREATMENT OF CARBON MONOXIDE POISONING

<130>  8123-104120-02

<150>  US 63/022,821
<151>  2020-05-11

<160>  14    

<170>  PatentIn version 3.5

<210>  1
<211>  267
<212>  PRT
<213>  Paraburkholderia xenovorans

<400>  1

Met Lys Ser Ser Glu Pro Ala Ser Val Ser Ala Ala Glu Arg Arg Ala 
1               5                   10                  15      


Glu Thr Phe Gln His Lys Leu Glu Gln Phe Asn Pro Gly Ile Val Trp 
            20                  25                  30          


Leu Asp Gln His Gly Arg Val Thr Ala Phe Asn Asp Val Ala Leu Gln 
        35                  40                  45              


Ile Leu Gly Pro Ala Gly Glu Gln Ser Leu Gly Val Ala Gln Asp Ser 
    50                  55                  60                  


Leu Phe Gly Ile Asp Val Val Gln Leu His Pro Glu Lys Ser Arg Asp 
65                  70                  75                  80  


Lys Leu Arg Phe Leu Leu Gln Ser Lys Asp Val Gly Gly Cys Pro Val 
                85                  90                  95      


Lys Ser Pro Pro Pro Val Ala Met Met Ile Asn Ile Pro Asp Arg Ile 
            100                 105                 110         


Leu Met Ile Lys Val Ser Ser Met Ile Ala Ala Gly Gly Ala Cys Gly 
        115                 120                 125             


Thr Cys Met Ile Phe Tyr Asp Val Thr Asp Leu Thr Thr Glu Pro Ser 
    130                 135                 140                 


Gly Leu Pro Ala Gly Gly Ser Ala Pro Ser Pro Arg Arg Leu Phe Lys 
145                 150                 155                 160 


Ile Pro Val Tyr Arg Lys Asn Arg Val Ile Leu Leu Asp Leu Lys Asp 
                165                 170                 175     


Ile Val Arg Phe Gln Gly Asp Gly His Tyr Thr Thr Ile Val Thr Arg 
            180                 185                 190         


Asp Asp Arg Tyr Leu Ser Asn Leu Ser Leu Ala Asp Leu Glu Leu Arg 
        195                 200                 205             


Leu Asp Ser Ser Ile Tyr Leu Arg Val His Arg Ser His Ile Val Ser 
    210                 215                 220                 


Leu Gln Tyr Ala Val Glu Leu Val Lys Leu Asp Glu Ser Val Asn Leu 
225                 230                 235                 240 


Val Met Asp Asp Ala Glu Gln Thr Gln Val Pro Val Ser Arg Ser Arg 
                245                 250                 255     


Thr Ala Gln Leu Lys Glu Leu Leu Gly Val Val 
            260                 265         


<210>  2
<211>  154
<212>  PRT
<213>  Paraburkholderia xenovorans

<400>  2

Met Lys Ser Ser Glu Pro Ala Ser Val Ser Ala Ala Glu Arg Arg Ala 
1               5                   10                  15      


Glu Thr Phe Gln His Lys Leu Glu Gln Phe Asn Pro Gly Ile Val Trp 
            20                  25                  30          


Leu Asp Gln His Gly Arg Val Thr Ala Phe Asn Asp Val Ala Leu Gln 
        35                  40                  45              


Ile Leu Gly Pro Ala Gly Glu Gln Ser Leu Gly Val Ala Gln Asp Ser 
    50                  55                  60                  


Leu Phe Gly Ile Asp Val Val Gln Leu His Pro Glu Lys Ser Arg Asp 
65                  70                  75                  80  


Lys Leu Arg Phe Leu Leu Gln Ser Lys Asp Val Gly Gly Cys Pro Val 
                85                  90                  95      


Lys Ser Pro Pro Pro Val Ala Met Met Ile Asn Ile Pro Asp Arg Ile 
            100                 105                 110         


Leu Met Ile Lys Val Ser Ser Met Ile Ala Ala Gly Gly Ala Cys Gly 
        115                 120                 125             


Thr Cys Met Ile Phe Tyr Asp Val Thr Asp Leu Thr Thr Glu Pro Ser 
    130                 135                 140                 


Gly Leu Pro Ala Gly Gly Ser Ala Pro Ser 
145                 150                 


<210>  3
<211>  108
<212>  PRT
<213>  Paraburkholderia xenovorans

<400>  3

Asn Pro Gly Ile Val Trp Leu Asp Gln His Gly Arg Val Thr Ala Phe 
1               5                   10                  15      


Asn Asp Val Ala Leu Gln Ile Leu Gly Pro Ala Gly Glu Gln Ser Leu 
            20                  25                  30          


Gly Val Ala Gln Asp Ser Leu Phe Gly Ile Asp Val Val Gln Leu His 
        35                  40                  45              


Pro Glu Lys Ser Arg Asp Lys Leu Arg Phe Leu Leu Gln Ser Lys Asp 
    50                  55                  60                  


Val Gly Gly Cys Pro Val Lys Ser Pro Pro Pro Val Ala Met Met Ile 
65                  70                  75                  80  


Asn Ile Pro Asp Arg Ile Leu Met Ile Lys Val Ser Ser Met Ile Ala 
                85                  90                  95      


Ala Gly Gly Ala Cys Gly Thr Cys Met Ile Phe Tyr 
            100                 105             


<210>  4
<211>  250
<212>  PRT
<213>  Hydrogenophaga crassostreae

<400>  4

Met Glu Ala Glu Val Ala Asn Lys Ser Pro Leu Tyr Leu Leu Glu Lys 
1               5                   10                  15      


Phe Glu Val Gly Val Ile His Leu Asp Ala Lys Arg Thr Val Leu Ala 
            20                  25                  30          


Met Asn Asp Phe Ala Arg Lys Val Leu Pro Val Gly Glu Lys Gln Pro 
        35                  40                  45              


Phe Asp Lys Leu Val Ser Ser Phe His Pro Ala Arg Ser Lys Pro Lys 
    50                  55                  60                  


Val Asp Phe Leu Leu Asp Gln Ala Ser Ser Cys Pro Met Val Ser Ala 
65                  70                  75                  80  


Val Pro Met Thr Met Ile Ile Asn Ile Pro Glu Gln Val Leu Leu Ile 
                85                  90                  95      


Lys Val Thr Arg Leu Ala Asp His Met Gly Lys Thr Thr Gly Phe Val 
            100                 105                 110         


Leu Val Phe Tyr Asp Val Thr Gln Val Val Ser Gln Glu Val Ala Ala 
        115                 120                 125             


Ser Glu Pro Pro Ser Thr Ser Val Arg Leu Thr Arg Ile Pro Met Val 
    130                 135                 140                 


Ala Asn His Lys Val Ala Phe Val Asp Thr Gln Asp Val Leu Cys Leu 
145                 150                 155                 160 


Glu Ser Gln Ala His Ser Thr Arg Ile Leu Thr Arg Asp Gly Phe His 
                165                 170                 175     


Phe Cys Asn Leu Ser Ile Gly Asp Leu Glu Ser Arg Leu Asp Pro Glu 
            180                 185                 190         


Gln Phe Met Arg Ile His Arg Cys Phe Ile Val Asn Leu Gln Gly Val 
        195                 200                 205             


Ala Glu Leu Gly Arg Glu Gly Ser Lys Thr His Val Val Leu Lys Gly 
    210                 215                 220                 


Lys Asn Lys Glu Pro Val Pro Val Ala Arg Gly Asp Val Leu Arg Leu 
225                 230                 235                 240 


Arg Lys Ala Leu Gly Leu Leu Ser Arg His 
                245                 250 


<210>  5
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic peptide


<220>
<221>  MISC_FEATURE
<222>  (7)..(7)
<223>  Xaa = Gly or Ser

<400>  5

Glu Asn Leu Tyr Phe Gln Xaa 
1               5           


<210>  6
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic peptide

<400>  6

Leu Val Pro Arg Gly Ser 
1               5       


<210>  7
<211>  154
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic polypeptide

<400>  7

Met Lys Ser Ser Glu Pro Ala Ser Val Ser Ala Ala Glu Arg Arg Ala 
1               5                   10                  15      


Glu Thr Phe Gln His Lys Leu Glu Gln Phe Asn Pro Gly Ile Val Trp 
            20                  25                  30          


Leu Asp Gln His Gly Arg Val Thr Ala Phe Asn Asp Val Ala Leu Gln 
        35                  40                  45              


Ile Leu Gly Pro Ala Gly Glu Gln Ser Leu Gly Val Ala Gln Asp Ser 
    50                  55                  60                  


Leu Phe Gly Ile Asp Val Val Gln Leu His Pro Glu Lys Ser Arg Asp 
65                  70                  75                  80  


Lys Leu Arg Phe Leu Leu Gln Ser Lys Asp Val Gly Gly Ser Pro Val 
                85                  90                  95      


Lys Ser Pro Pro Pro Val Ala Met Met Ile Asn Ile Pro Asp Arg Ile 
            100                 105                 110         


Leu Met Ile Lys Val Ser Ser Met Ile Ala Ala Gly Gly Ala Cys Gly 
        115                 120                 125             


Thr Cys Met Ile Phe Tyr Asp Val Thr Asp Leu Thr Thr Glu Pro Ser 
    130                 135                 140                 


Gly Leu Pro Ala Gly Gly Ser Ala Pro Ser 
145                 150                 


<210>  8
<211>  154
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic polypeptide

<400>  8

Met Lys Ser Ser Glu Pro Ala Ser Val Ser Ala Ala Glu Arg Arg Ala 
1               5                   10                  15      


Glu Thr Phe Gln His Lys Leu Glu Gln Phe Asn Pro Gly Ile Val Trp 
            20                  25                  30          


Leu Asp Gln His Gly Arg Val Thr Ala Phe Asn Asp Val Ala Leu Gln 
        35                  40                  45              


Ile Leu Gly Pro Ala Gly Glu Gln Ser Leu Gly Val Ala Gln Asp Ser 
    50                  55                  60                  


Leu Phe Gly Ile Asp Val Val Gln Leu His Pro Glu Lys Ser Arg Asp 
65                  70                  75                  80  


Lys Leu Arg Phe Leu Leu Gln Ser Lys Asp Val Gly Gly Cys Pro Val 
                85                  90                  95      


Lys Ser Pro Pro Pro Val Ala Met Met Ile Asn Ile Pro Asp Arg Ile 
            100                 105                 110         


Leu Met Ile Lys Val Ser Ser Met Ile Ala Ala Gly Gly Ala Ser Gly 
        115                 120                 125             


Thr Ser Met Ile Phe Tyr Asp Val Thr Asp Leu Thr Thr Glu Pro Ser 
    130                 135                 140                 


Gly Leu Pro Ala Gly Gly Ser Ala Pro Ser 
145                 150                 


<210>  9
<211>  154
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic polypeptide

<400>  9

Met Lys Ser Ser Glu Pro Ala Ser Val Ser Ala Ala Glu Arg Arg Ala 
1               5                   10                  15      


Glu Thr Phe Gln His Lys Leu Glu Gln Phe Asn Pro Gly Ile Val Trp 
            20                  25                  30          


Leu Asp Gln His Gly Arg Val Thr Ala Phe Asn Asp Val Ala Leu Gln 
        35                  40                  45              


Ile Leu Gly Pro Ala Gly Glu Gln Ser Leu Gly Val Ala Gln Asp Ser 
    50                  55                  60                  


Leu Phe Gly Ile Asp Val Val Gln Leu His Pro Glu Lys Ser Arg Asp 
65                  70                  75                  80  


Lys Leu Arg Phe Leu Leu Gln Ser Lys Asp Val Gly Gly Ser Pro Val 
                85                  90                  95      


Lys Ser Pro Pro Pro Val Ala Met Met Ile Asn Ile Pro Asp Arg Ile 
            100                 105                 110         


Leu Met Ile Lys Val Ser Ser Met Ile Ala Ala Gly Gly Ala Ser Gly 
        115                 120                 125             


Thr Ser Met Ile Phe Tyr Asp Val Thr Asp Leu Thr Thr Glu Pro Ser 
    130                 135                 140                 


Gly Leu Pro Ala Gly Gly Ser Ala Pro Ser 
145                 150                 


<210>  10
<211>  154
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic polypeptide

<400>  10

Met Lys Ser Ser Glu Pro Ala Ser Val Ser Ala Ala Glu Arg Arg Ala 
1               5                   10                  15      


Glu Thr Phe Gln His Lys Leu Glu Gln Phe Asn Pro Gly Ile Val Trp 
            20                  25                  30          


Leu Asp Gln His Gly Arg Val Thr Ala Phe Asn Asp Val Ala Leu Gln 
        35                  40                  45              


Ile Leu Gly Pro Ala Gly Glu Gln Ser Leu Gly Val Ala Gln Asp Ser 
    50                  55                  60                  


Leu Phe Gly Ile Asp Val Val Gln Leu His Pro Glu Lys Ser Arg Asp 
65                  70                  75                  80  


Lys Leu Arg Phe Leu Leu Gln Ser Lys Asp Val Gly Gly Cys Pro Val 
                85                  90                  95      


Lys Ser Pro Pro Pro Val Ala Ala Met Ile Asn Ile Pro Asp Arg Ile 
            100                 105                 110         


Leu Met Ile Lys Val Ser Ser Met Ile Ala Ala Gly Gly Ala Ser Gly 
        115                 120                 125             


Thr Ser Met Ile Phe Tyr Asp Val Thr Asp Leu Thr Thr Glu Pro Ser 
    130                 135                 140                 


Gly Leu Pro Ala Gly Gly Ser Ala Pro Ser 
145                 150                 


<210>  11
<211>  154
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic polypeptide

<400>  11

Met Lys Ser Ser Glu Pro Ala Ser Val Ser Ala Ala Glu Arg Arg Ala 
1               5                   10                  15      


Glu Thr Phe Gln His Lys Leu Glu Gln Phe Asn Pro Gly Ile Val Trp 
            20                  25                  30          


Leu Asp Gln His Gly Arg Val Thr Ala Phe Asn Asp Val Ala Leu Gln 
        35                  40                  45              


Ile Leu Gly Pro Ala Gly Glu Gln Ser Leu Gly Val Ala Gln Asp Ser 
    50                  55                  60                  


Leu Phe Gly Ile Asp Val Val Gln Leu His Pro Glu Lys Ser Arg Asp 
65                  70                  75                  80  


Lys Leu Arg Phe Leu Leu Gln Ser Lys Asp Val Gly Gly Cys Pro Val 
                85                  90                  95      


Lys Ser Pro Pro Pro Val Ala His Met Ile Asn Ile Pro Asp Arg Ile 
            100                 105                 110         


Leu Met Ile Lys Val Ser Ser Met Ile Ala Ala Gly Gly Ala Ser Gly 
        115                 120                 125             


Thr Ser Met Ile Phe Tyr Asp Val Thr Asp Leu Thr Thr Glu Pro Ser 
    130                 135                 140                 


Gly Leu Pro Ala Gly Gly Ser Ala Pro Ser 
145                 150                 


<210>  12
<211>  154
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic polypeptide

<400>  12

Met Lys Ser Ser Glu Pro Ala Ser Val Ser Ala Ala Glu Arg Arg Ala 
1               5                   10                  15      


Glu Thr Phe Gln His Lys Leu Glu Gln Phe Asn Pro Gly Ile Val Trp 
            20                  25                  30          


Leu Asp Gln His Gly Arg Val Thr Ala Phe Asn Asp Val Ala Leu Gln 
        35                  40                  45              


Ile Leu Gly Pro Ala Gly Glu Gln Ser Leu Gly Val Ala Gln Asp Ser 
    50                  55                  60                  


Leu Phe Gly Ile Asp Val Val Gln Leu His Pro Glu Lys Ser Arg Asp 
65                  70                  75                  80  


Lys Leu Arg Phe Leu Leu Gln Ser Lys Asp Val Gly Gly Ser Pro Val 
                85                  90                  95      


Lys Ser Pro Pro Pro Val Ala Ala Met Ile Asn Ile Pro Asp Arg Ile 
            100                 105                 110         


Leu Met Ile Lys Val Ser Ser Met Ile Ala Ala Gly Gly Ala Ser Gly 
        115                 120                 125             


Thr Ser Met Ile Phe Tyr Asp Val Thr Asp Leu Thr Thr Glu Pro Ser 
    130                 135                 140                 


Gly Leu Pro Ala Gly Gly Ser Ala Pro Ser 
145                 150                 


<210>  13
<211>  154
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic polypeptide

<400>  13

Met Lys Ser Ser Glu Pro Ala Ser Val Ser Ala Ala Glu Arg Arg Ala 
1               5                   10                  15      


Glu Thr Phe Gln His Lys Leu Glu Gln Phe Asn Pro Gly Ile Val Trp 
            20                  25                  30          


Leu Asp Gln His Gly Arg Val Thr Ala Phe Asn Asp Val Ala Leu Gln 
        35                  40                  45              


Ile Leu Gly Pro Ala Gly Glu Gln Ser Leu Gly Val Ala Gln Asp Ser 
    50                  55                  60                  


Leu Phe Gly Ile Asp Val Val Gln Leu His Pro Glu Lys Ser Arg Asp 
65                  70                  75                  80  


Lys Leu Arg Phe Leu Leu Gln Ser Lys Asp Val Gly Gly Ser Pro Val 
                85                  90                  95      


Lys Ser Pro Pro Pro Val Ala His Met Ile Asn Ile Pro Asp Arg Ile 
            100                 105                 110         


Leu Met Ile Lys Val Ser Ser Met Ile Ala Ala Gly Gly Ala Ser Gly 
        115                 120                 125             


Thr Ser Met Ile Phe Tyr Asp Val Thr Asp Leu Thr Thr Glu Pro Ser 
    130                 135                 140                 


Gly Leu Pro Ala Gly Gly Ser Ala Pro Ser 
145                 150                 


<210>  14
<211>  154
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic polypeptide

<400>  14

Met Lys Ser Ser Glu Pro Ala Ser Val Ser Ala Ala Glu Arg Arg Ala 
1               5                   10                  15      


Glu Thr Phe Gln His Lys Leu Glu Gln Phe Asn Pro Gly Ile Val Trp 
            20                  25                  30          


Leu Asp Gln His Gly Arg Val Thr Ala Phe Asn Asp Val Ala Leu Gln 
        35                  40                  45              


Ile Leu Gly Pro Ala Gly Glu Gln Ser Leu Gly Val Ala Gln Asp Ser 
    50                  55                  60                  


Leu Phe Gly Ile Asp Val Val Gln Leu His Pro Glu Lys Ser Arg Asp 
65                  70                  75                  80  


Lys Leu Arg Phe Leu Leu Gln Ser Lys Asp Val Gly Gly Ser Pro Val 
                85                  90                  95      


Lys Ser Pro Pro Pro Val Ala Leu Met Ile Asn Ile Pro Asp Arg Ile 
            100                 105                 110         


Leu Met Ile Lys Val Ser Ser Met Ile Ala Ala Gly Gly Ala Ser Gly 
        115                 120                 125             


Thr Ser Met Ile Phe Tyr Asp Val Thr Asp Leu Thr Thr Glu Pro Ser 
    130                 135                 140                 


Gly Leu Pro Ala Gly Gly Ser Ala Pro Ser 
145                 150                 


