                         SEQUENCE LISTING

<110>  Lycke, Nils
 
<120>  FUSION PROTEIN WITH IMMUNOENHANCING ACTIVITY

<130>  HSJ103117P.WOP

<150>  US 63/025,550
<151>  2020-05-15

<160>  43    

<170>  PatentIn version 3.5

<210>  1
<211>  592
<212>  DNA
<213>  Vibrio cholerae

<400>  1
atggatgata agttatatcg ggcagattct agacctcctg atgaaataaa gcagtcaggt       60

ggtcttatgc caagaggaca gagtgagtac tttgaccgag gtactcaaat gaatatcaac      120

ctttatgatc atgcaagagg aactcagacg ggatttgtta ggcacgatga tggatatgtt      180

tccacctcaa ttagtttgag aagtgcccac ttagtgggtc aaactatatt gtctggtcat      240

tctacttatt atatatatgt tatagccact gcacccaaca tgtttaacgt taatgatgta      300

ttaggggcat acagtcctca tccagatgaa caagaagttt ctgctttagg tgggattcca      360

tactcccaaa tatatggatg gtatcgagtt cattttgggg tgcttgatga acaattacat      420

cgtaataggg gctacagaga tagatattac agtaacttag atattgctcc agcagcagat      480

ggttatggat tggcaggttt ccctccggag catagagctt ggagggaaga gccgtggatt      540

catcatgcac cgccgggttg tgggaatgct ccaagatcat cgggatctac ta              592


<210>  2
<211>  774
<212>  DNA
<213>  Escherichia coli

<400>  2
atgaagaaca tcaccttcat cttcttcatc ctgctggcca gccccctgta cgccaacggc       60

gacaagctgt acagggccga cagcaggccc cccgacgaga tcaagcacag cggcggcctg      120

atgcccaggg gccacaacga gtacttcgac aggggcaccc agatcaacat caacctgtac      180

gaccacgcca ggggcaccca gaccggcttc gtgaggtacg acgacggcta cgtgagcacc      240

agcctgagcc tgaggagcgc ccacctggcc ggccagagca tcctgagcgg ctacagcacc      300

tactacatct acgtgatcgc caccgccccc aacatgttca acgtgaacga cgtgctgggc      360

gtgtacagcc cccaccccta cgagcaggag gtgagcgccc tgggcggcat cccctacagc      420

cagatctacg gctggtacag ggtgaacttc ggcgtgatcg acgagaggct gcacaggaac      480

agggagtaca gggacaggta ctacaggaac ctgaacatcg cccccgccga ggacggctac      540

aggctggccg gcttcccccc cgaccaccag gcctggaggg aggagccctg gatccaccac      600

gccccccagg gctgcggcaa cagcagcagg accatcaccg gcgacacctg caacgaggag      660

acccagaacc tgagcaccat ctacctgagg aagtaccaga gcaaggtgaa gaggcagatc      720

ttcagcgact accagagcga ggtggacatc tacaacagga tcagggacga gctg            774


<210>  3
<211>  705
<212>  DNA
<213>  Bordetella pertussis

<400>  3
gacgatcctc ccgccaccgt ataccgctat gactcccgcc cgccggagga cgttttccag       60

aacggattca cggcgtgggg aaacaacgac aatgtgctcg accatctgac cggacgttcc      120

tgccaggtcg gcagcagcaa cagcgctttc gtctccacca gcagcagccg gcgctatacc      180

gaggtctatc tcgaacatcg catgcaggaa gcggtcgagg ccgaacgcgc cggcaggggc      240

accggccact tcatcggcta catctacgaa gtccgcgccg acaacaattt ctacggcgcc      300

gccagctcgt acttcgaata cgtcgacact tatggcgaca atgccggccg tatcctcgcc      360

ggcgcgctgg ccacctacca gagcgaatat ctggcacacc ggcgcattcc gcccgaaaac      420

atccgcaggg taacgcgggt ctatcacaac ggcatcaccg gcgagaccac gaccacggag      480

tattccaacg ctcgctacgt cagccagcag actcgcgcca atcccaaccc ctacacatcg      540

cgaaggtccg tagcgtcgat cgtcggcaca ttggtgcgca tggcgccggt gataggcgct      600

tgcatggcgc ggcaggccga aagctccgag gccatggcag cctggtccga acgcgccggc      660

gaggcgatgg ttctcgtgta ctacgaaagc atcgcgtatt cgttc                      705


<210>  4
<211>  765
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CD103-binding scFv

<400>  4
cccggggagg tgcagctggt ggagtctggg ggaggcttag tgcagcctgg aaggtccctg       60

aaactctcct gtgcagcctc aggattcact ttcagtgact attacatggc ctgggtccgc      120

cagtctccaa agaagggtct ggagtgggtc gcatccatta gttatgaggg tactgacact      180

tactatggag actccgtgaa gggccgattc actatctcca gagataatgc aaaaagcacc      240

ctgtacctgc aaatgaacag tctgaggtct gaggacacgg ccacttatta ttgtgcaagc      300

ttgaatggct ataactatcg atactttgac ttctggggcc caggaaccat ggtcaccgtg      360

tcctcaggtg gaggcggttc aggcggaggt ggctctggcg gtggagggag cggtggaggg      420

agtaacatcc agatgaccca gtctccttca atactgtctg catctgtggg agacagagtc      480

actctcagct gcaaagcagg tcagaatatt aacaatttct tagcctggta tcagcaaaag      540

cttggagaag ctcccaaact cctgatatat aatgcaaaca gtttggaaac gggcatccca      600

tcaaggttca gtggcagtgg atctggcaca gattacacac tcaccatcag cagcctgcgg      660

cctgaagatg ttgccacata tttctgccag cagtataaca gttggtacac gtttggagct      720

gggaccaagc tggaactgaa acgggctgat gcggccgcaa agctt                      765


<210>  5
<211>  778
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CD205-binding scFv

<400>  5
caggtcaagc tgcaggagtc aggaggaggt ttggtacagc cggggggttc tctgagactc       60

tcctgtgcag cttctggatt caccttcaat gatttctaca tgaactggat ccgccagcct      120

ccagggcagg cacctgagtg gttgggtgtt attagaaaca aaggtaatgg ttacacaaca      180

gaggtcaata catctgtgaa ggggcggttc accatctcca gagataatac ccaaaacatc      240

ctctatcttc aaatgaacag cctgagagct gaggacaccg ccatttacta ctgtgcaaga      300

ggcggtcctt attactacag tggtgacgac gccccttact ggggccaagg gaccacggtc      360

accgtctcga gtggtggagg cggttcaggc ggaggtggct ctggcggtgg agggagcggt      420

ggagggagtg cagacattgt gctcactcaa tctccagctt ccttagctgt atctctgggg      480

cagagggcca ccatctcatg cagggccagc aaaagtgtca gtacatctgg ctatagttat      540

atgcactgga accaacagaa accaggacag ccacccagac tcctcatcta tcttgtatcc      600

aacctagaat ctggggtccc tgccaggttc agtggcagtg ggtctgggac agacttcacc      660

ctcaacatcc atcctgtgga ggaggaggat gctgcaacct attactgtca gcacattagg      720

gagctttaca cgttcggagg ggggaccaag ctggaaataa aacgggcggc cgcataga        778


<210>  6
<211>  16
<212>  PRT
<213>  Influenza A virus

<400>  6

Ser Leu Leu Thr Glu Val Glu Thr Pro Ile Arg Asn Glu Trp Gly Ser 
1               5                   10                  15      


<210>  7
<211>  23
<212>  PRT
<213>  Influenza A virus

<400>  7

Ser Leu Leu Thr Glu Val Glu Thr Pro Ile Arg Asn Glu Trp Gly Cys 
1               5                   10                  15      


Arg Cys Asn Asp Ser Ser Asp 
            20              


<210>  8
<211>  23
<212>  PRT
<213>  Influenza A virus

<400>  8

Met Ser Leu Leu Thr Glu Val Glu Thr Pro Thr Arg Asn Glu Trp Glu 
1               5                   10                  15      


Cys Arg Cys Ser Asp Ser Ser 
            20              


<210>  9
<211>  48
<212>  DNA
<213>  Influenza A virus

<400>  9
agcctgctga ccgaggtgga gacccccatc aggaacgagt ggggcagc                    48


<210>  10
<211>  69
<212>  DNA
<213>  Influenza A virus

<400>  10
agcctgctga ccgaggtgga gacccccatc aggaacgagt ggggctgcag gtgcaacgac       60

agcagcgac                                                               69


<210>  11
<211>  69
<212>  DNA
<213>  Influenza A virus

<400>  11
atgagcctgc tgaccgaggt ggagaccccc accaggaacg agtgggagtg caggtgcagc       60

gacagcagc                                                               69


<210>  12
<211>  1869
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nt sequence encoding CTA1 - 3M2e - CD103-binding scFv

<400>  12
atgaaatttt tggtgaacgt ggccttggtg tttatggtgg tgtacatttc gtacatttac       60

gccgacccgt cgccggacga caaattgtac agagccgact cgagaccgcc ggacgaaatt      120

aaacaatcgg gcggcttgat gccgagaggc caatcggaat actttgacag aggcacgcaa      180

atgaacatta acttgtacga ccacgccaga ggcacgcaaa cgggctttgt gagacacgac      240

gacggctacg tgtcgacgtc gatttcgttg agatcggccc acttggtggg ccaaacgatt      300

ttgtcgggcc actcgacgta ctacatttac gtgattgcca cggccccgaa catgtttaac      360

gtgaacgacg tgttgggcgc ctactcgccg cacccggacg aacaagaagt gtcggccttg      420

ggcggcattc cgtactcgca aatttacggc tggtacagag tgcactttgg cgtgttggac      480

gaacaattgc acagaaacag aggctacaga gacagatact actcgaactt ggacattgcc      540

ccggccgccg acggctacgg cttggccggc tttccgccgg aacacagagc ctggagagaa      600

gaaccgtgga ttcaccacgc cccgccgggc tgtggcaacg ccccgagatc gtcggggaat      660

tcgttgttga cggaagtgga aacgccgatt agaaacgaat ggggctcgag atcgaacgac      720

tcgtcgttgt tgacggaagt ggaaacgccg attagaaacg aatggggctc gagatcgaac      780

gactcgtcgt tgttgacgga agtggaaacg ccgattagaa acgaatgggg ctcgagatcg      840

aacgactcgt cggacggttt aattaagttt aaaccatatg aagccagctt cgaggcccag      900

ggcgccctgg ccaacatcgc cgtggacaag gccgccagct tcgaggccca gggcgccctg      960

gccaacatcg ccgtggacaa ggccgccagc ttcgaggccc agggcgccct ggccaacatc     1020

gccgtggaca aggccagttt aaaccccggg agcggggagg tgcagctggt ggagtctggg     1080

ggaggcttag tgcagcctgg aaggtccctg aaactctcct gtgcagcctc aggattcact     1140

ttcagtgact attacatggc ctgggtccgc cagtctccaa agaagggtct ggagtgggtc     1200

gcatccatta gttatgaggg tactgacact tactatggag actccgtgaa gggccgattc     1260

actatctcca gagataatgc aaaaagcacc ctgtacctgc aaatgaacag tctgaggtct     1320

gaggacacgg ccacttatta ttgtgcaagc ttgaatggct ataactatcg atactttgac     1380

ttctggggcc caggaaccat ggtcaccgtg tcctcaggtg gaggcggttc aggcggaggt     1440

ggctctggcg gtggagggag cggtggaggg agtaacatcc agatgaccca gtctccttca     1500

atactgtctg catctgtggg agacagagtc actctcagct gcaaagcagg tcagaatatt     1560

aacaattttt tagcctggta tcagcaaaag cttggagaag ctcccaaact cctgatatat     1620

aatgcaaaca gtttggaaac gggcatccca tcaaggttca gtggcagtgg atttggcaca     1680

gattacacac tcaccatcag cagcctgcgg cctgaagatg ttgccacata tttttgccag     1740

cagtataaca gttggtacac gtttggaggt gggaccaagc tggaaatgaa acgggctgat     1800

gcggccgcat attcgaagag ctccgactac aaagacgacg acgacaaaca ccaccaccac     1860

caccactag                                                             1869


<210>  13
<211>  197
<212>  PRT
<213>  Vibrio cholerae

<400>  13

Met Asp Asp Lys Leu Tyr Arg Ala Asp Ser Arg Pro Pro Asp Glu Ile 
1               5                   10                  15      


Lys Gln Ser Gly Gly Leu Met Pro Arg Gly Gln Ser Glu Tyr Phe Asp 
            20                  25                  30          


Arg Gly Thr Gln Met Asn Ile Asn Leu Tyr Asp His Ala Arg Gly Thr 
        35                  40                  45              


Gln Thr Gly Phe Val Arg His Asp Asp Gly Tyr Val Ser Thr Ser Ile 
    50                  55                  60                  


Ser Leu Arg Ser Ala His Leu Val Gly Gln Thr Ile Leu Ser Gly His 
65                  70                  75                  80  


Ser Thr Tyr Tyr Ile Tyr Val Ile Ala Thr Ala Pro Asn Met Phe Asn 
                85                  90                  95      


Val Asn Asp Val Leu Gly Ala Tyr Ser Pro His Pro Asp Glu Gln Glu 
            100                 105                 110         


Val Ser Ala Leu Gly Gly Ile Pro Tyr Ser Gln Ile Tyr Gly Trp Tyr 
        115                 120                 125             


Arg Val His Phe Gly Val Leu Asp Glu Gln Leu His Arg Asn Arg Gly 
    130                 135                 140                 


Tyr Arg Asp Arg Tyr Tyr Ser Asn Leu Asp Ile Ala Pro Ala Ala Asp 
145                 150                 155                 160 


Gly Tyr Gly Leu Ala Gly Phe Pro Pro Glu His Arg Ala Trp Arg Glu 
                165                 170                 175     


Glu Pro Trp Ile His His Ala Pro Pro Gly Cys Gly Asn Ala Pro Arg 
            180                 185                 190         


Ser Ser Gly Ser Thr 
        195         


<210>  14
<211>  212
<212>  PRT
<213>  Escherichia coli

<400>  14

Met Lys Asn Ile Thr Phe Ile Phe Phe Ile Leu Leu Ala Ser Pro Leu 
1               5                   10                  15      


Tyr Ala Asn Gly Asp Lys Leu Tyr Arg Ala Asp Ser Arg Pro Pro Asp 
            20                  25                  30          


Glu Ile Lys His Ser Gly Gly Leu Met Pro Arg Gly His Asn Glu Tyr 
        35                  40                  45              


Phe Asp Arg Gly Thr Gln Ile Asn Ile Asn Leu Tyr Asp His Ala Arg 
    50                  55                  60                  


Gly Thr Gln Thr Gly Phe Val Arg Tyr Asp Asp Gly Tyr Val Ser Thr 
65                  70                  75                  80  


Ser Leu Ser Leu Arg Ser Ala His Leu Ala Gly Gln Ser Ile Leu Ser 
                85                  90                  95      


Gly Tyr Ser Thr Tyr Tyr Ile Tyr Val Ile Ala Thr Ala Pro Asn Met 
            100                 105                 110         


Phe Asn Val Asn Asp Val Leu Gly Val Tyr Ser Pro His Pro Tyr Glu 
        115                 120                 125             


Gln Glu Val Ser Ala Leu Gly Gly Ile Pro Tyr Ser Gln Ile Tyr Gly 
    130                 135                 140                 


Trp Tyr Arg Val Asn Phe Gly Val Ile Asp Glu Arg Leu His Arg Asn 
145                 150                 155                 160 


Arg Glu Tyr Arg Asp Arg Tyr Tyr Arg Asn Leu Asn Ile Ala Pro Ala 
                165                 170                 175     


Glu Asp Gly Tyr Arg Leu Ala Gly Phe Pro Pro Asp His Gln Ala Trp 
            180                 185                 190         


Arg Glu Glu Pro Trp Ile His His Ala Pro Gln Gly Cys Gly Asn Ser 
        195                 200                 205             


Ser Arg Thr Ile 
    210         


<210>  15
<211>  235
<212>  PRT
<213>  Bordetella pertussis

<400>  15

Asp Asp Pro Pro Ala Thr Val Tyr Arg Tyr Asp Ser Arg Pro Pro Glu 
1               5                   10                  15      


Asp Val Phe Gln Asn Gly Phe Thr Ala Trp Gly Asn Asn Asp Asn Val 
            20                  25                  30          


Leu Asp His Leu Thr Gly Arg Ser Cys Gln Val Gly Ser Ser Asn Ser 
        35                  40                  45              


Ala Phe Val Ser Thr Ser Ser Ser Arg Arg Tyr Thr Glu Val Tyr Leu 
    50                  55                  60                  


Glu His Arg Met Gln Glu Ala Val Glu Ala Glu Arg Ala Gly Arg Gly 
65                  70                  75                  80  


Thr Gly His Phe Ile Gly Tyr Ile Tyr Glu Val Arg Ala Asp Asn Asn 
                85                  90                  95      


Phe Tyr Gly Ala Ala Ser Ser Tyr Phe Glu Tyr Val Asp Thr Tyr Gly 
            100                 105                 110         


Asp Asn Ala Gly Arg Ile Leu Ala Gly Ala Leu Ala Thr Tyr Gln Ser 
        115                 120                 125             


Glu Tyr Leu Ala His Arg Arg Ile Pro Pro Glu Asn Ile Arg Arg Val 
    130                 135                 140                 


Thr Arg Val Tyr His Asn Gly Ile Thr Gly Glu Thr Thr Thr Thr Glu 
145                 150                 155                 160 


Tyr Ser Asn Ala Arg Tyr Val Ser Gln Gln Thr Arg Ala Asn Pro Asn 
                165                 170                 175     


Pro Tyr Thr Ser Arg Arg Ser Val Ala Ser Ile Val Gly Thr Leu Val 
            180                 185                 190         


Arg Met Ala Pro Val Ile Gly Ala Cys Met Ala Arg Gln Ala Glu Ser 
        195                 200                 205             


Ser Glu Ala Met Ala Ala Trp Ser Glu Arg Ala Gly Glu Ala Met Val 
    210                 215                 220                 


Leu Val Tyr Tyr Glu Ser Ile Ala Tyr Ser Phe 
225                 230                 235 


<210>  16
<211>  253
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CD103-binding scFv

<400>  16

Pro Gly Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro 
1               5                   10                  15      


Gly Arg Ser Leu Lys Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser 
            20                  25                  30          


Asp Tyr Tyr Met Ala Trp Val Arg Gln Ser Pro Lys Lys Gly Leu Glu 
        35                  40                  45              


Trp Val Ala Ser Ile Ser Tyr Glu Gly Thr Asp Thr Tyr Tyr Gly Asp 
    50                  55                  60                  


Ser Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys Ser Thr 
65                  70                  75                  80  


Leu Tyr Leu Gln Met Asn Ser Leu Arg Ser Glu Asp Thr Ala Thr Tyr 
                85                  90                  95      


Tyr Cys Ala Ser Leu Asn Gly Tyr Asn Tyr Arg Tyr Phe Asp Phe Trp 
            100                 105                 110         


Gly Pro Gly Thr Met Val Thr Val Ser Ser Gly Gly Gly Gly Ser Gly 
        115                 120                 125             


Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Ser Asn Ile Gln 
    130                 135                 140                 


Met Thr Gln Ser Pro Ser Ile Leu Ser Ala Ser Val Gly Asp Arg Val 
145                 150                 155                 160 


Thr Leu Ser Cys Lys Ala Gly Gln Asn Ile Asn Asn Phe Leu Ala Trp 
                165                 170                 175     


Tyr Gln Gln Lys Leu Gly Glu Ala Pro Lys Leu Leu Ile Tyr Asn Ala 
            180                 185                 190         


Asn Ser Leu Glu Thr Gly Ile Pro Ser Arg Phe Ser Gly Ser Gly Ser 
        195                 200                 205             


Gly Thr Asp Tyr Thr Leu Thr Ile Ser Ser Leu Arg Pro Glu Asp Val 
    210                 215                 220                 


Ala Thr Tyr Phe Cys Gln Gln Tyr Asn Ser Trp Tyr Thr Phe Gly Ala 
225                 230                 235                 240 


Gly Thr Lys Leu Glu Leu Lys Arg Ala Asp Ala Ala Ala 
                245                 250             


<210>  17
<211>  22
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  SARS-CoV-2

<400>  17

Phe Gly Ala Gly Ala Ala Leu Gln Ile Pro Phe Ala Met Gln Met Ala 
1               5                   10                  15      


Tyr Arg Phe Asn Gly Ile 
            20          


<210>  18
<211>  18
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  SARS-CoV-2

<400>  18

Gln Leu Ile Arg Ala Ala Glu Ile Arg Ala Ser Ala Asn Leu Ala Ala 
1               5                   10                  15      


Thr Lys 
        


<210>  19
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  SARS-CoV-2

<400>  19

Met Met Ile Ser Ala Gly Phe Ser Leu 
1               5                   


<210>  20
<211>  60
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SARS-CoV-2

<400>  20
tttggcgcgg gcgcggcgct gcagattccg tttgcgatgc agatggcgta tcgctttaac       60


<210>  21
<211>  54
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SARS-CoV-2

<400>  21
cagctgattc gcgcggcgga aattcgcgcg agcgcgaacc tggcggcgac caaa             54


<210>  22
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SARS-CoV-2

<400>  22
atgatgatta gcgcgggctt tagcctg                                           27


<210>  23
<211>  468
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Amplified cDNA from M290 hybridoma

<400>  23
gatagataca gttggtgcag catcagcccg tttcatttcc agcttggtcc cacctccaaa       60

cgtgtaccaa ctgttatact gctggcaaaa atatgtggca acatcttcag gccgcaggct      120

gctgatggtg agtgtgtaat ctgtgccaaa tccactgcca ctgaaccttg atgggatgcc      180

cgtttccaaa ctgtttgcat tatatatcag gagtttggga gcttctccaa gcttttgctg      240

ataccaggct aaaaaattgt taatattctg acctgctttg cagctgagag tgactctgtc      300

tcccacagat gcagacagta ttgaaggaga ctgggtcatc tggatgttac atctcagggc      360

tgggagccag agcaacacca gccctaagag ttgaactgga gccatcatga ctggcctgtg      420

tcctgtctga gactgaatac caaagcctgc cggggggggg ggggaaca                   468


<210>  24
<211>  564
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Amplified cDNA from M290 hybridoma

<400>  24
taagtacttt tgagagcagt tccaggagcc agtggataga cagatggggc tgttgtttca       60

gctgaggaca cggtgaccat ggttcctggg ccccagaagt caaagtatcg atagttatag      120

ccattcaagc ttgcacaata ataagtggcc gtgtcctcag acctcagact gttcatttgc      180

aggtacaggg tgctttttgc attatctctg gagatagtga atcggccctt cacggagtct      240

ccatagtaag tgtcagtacc ctcataacta atggatgcga cccactccag acccttcttt      300

ggagactggc ggacccaggc catgtaatag tcactgaaag tgaatcctga ggctgcacag      360

gagagtttca gggaccttcc aggctgcact aagcctcccc cagactccac cagctgcacc      420

tcacactgga caccttttat gaaaaggaca aggaaaacca agctgagcct ggtgtccatg      480

gtgagtagtc tgtgcagtgc tgcagtactg attactgaat gggagagcct cagagtccag      540

gactgggctc ctgcgtccga gggg                                             564


<210>  25
<211>  43
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  M290 VH forward primer

<400>  25
ggcggataga tctactagtc ccggggaggt gcagctggtg gag                         43


<210>  26
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  M290 VH reverse primer

<400>  26
tgaggacacg gtgaccatgg ttcctgggcc cca                                    33


<210>  27
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  M290 VL forward primer

<400>  27
aacatccaga tgacccagtc tccttcaata ctg                                    33


<210>  28
<211>  42
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  M290 VL reverse primer

<400>  28
gccgccaagc ttctatgcgg ccgcgaaatg aaacgggctg at                          42


<210>  29
<211>  366
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  VH sequence

<400>  29
cccggggagg tgcagctggt ggagtctggg ggaggcttag tgcagcctgg aaggtccctg       60

aaactctcct gtgcagcctc aggattcact ttcagtgact attacatggc ctgggtccgc      120

cagtctccaa agaagggtct ggagtgggtc gcatccatta gttatgaggg tactgacact      180

tactatggag actccgtgaa gggccgattc actatctcca gagataatgc aaaaagcacc      240

ctgtacctgc aaatgaacag tctgaggtct gaggacacgg ccacttatta ttgtgcaagc      300

ttgaatggct ataactatcg atactttgac ttctggggcc caggaaccat ggtcaccgtg      360

tcctca                                                                 366


<210>  30
<211>  351
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  VL sequence

<400>  30
aacatccaga tgacccagtc tccttcaata ctgtctgcat ctgtgggaga cagagtcact       60

ctcagctgca aagcaggtca gaatattaac aattttttag cctggtatca gcaaaagctt      120

ggagaagctc ccaaactcct gatatataat gcaaacagtt tggaaacggg catcccatca      180

aggttcagtg gcagtggatt tggcacagat tacacactca ccatcagcag cctgcggcct      240

gaagatgttg ccacatattt ttgccagcag tataacagtt ggtacacgtt tggaggtggg      300

accaagctgg aaatgaaacg ggctgatgcg gccgcataga agcttggcgg c               351


<210>  31
<211>  87
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  GS-M290 forward primer

<400>  31
gtcaccgtgt cctcaggtgg aggcggttca ggcggaggtg gctctggcgg tggagggagc       60

ggtggaggga gtaacatcca gatgacc                                           87


<210>  32
<211>  87
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  GS-M290 reverse primer

<400>  32
ggtcatctgg atgttactcc ctccaccgct ccctccaccg ccagagccac ctccgcctga       60

accgcctcca cctgaggaca cggtgac                                           87


<210>  33
<211>  793
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CD103 scFv

<400>  33
ggcggataga tctactagtc ccggggaggt gcagctggtg gagtctgggg gaggcttagt       60

gcagcctgga aggtccctga aactctcctg tgcagcctca ggattcactt tcagtgacta      120

ttacatggcc tgggtccgcc agtctccaaa gaagggtctg gagtgggtcg catccattag      180

ttatgagggt actgacactt actatggaga ctccgtgaag ggccgattca ctatctccag      240

agataatgca aaaagcaccc tgtacctgca aatgaacagt ctgaggtctg aggacacggc      300

cacttattat tgtgcaagct tgaatggcta taactatcga tactttgact tctggggccc      360

aggaaccatg gtcaccgtgt cctcaggtgg aggcggttca ggcggaggtg gctctggcgg      420

tggagggagc ggtggaggga gtaacatcca gatgacccag tctccttcaa tactgtctgc      480

atctgtggga gacagagtca ctctcagctg caaagcaggt cagaatatta acaatttttt      540

agcctggtat cagcaaaagc ttggagaagc tcccaaactc ctgatatata atgcaaacag      600

tttggaaacg ggcatcccat caaggttcag tggcagtgga tttggcacag attacacact      660

caccatcagc agcctgcggc ctgaagatgt tgccacatat ttttgccagc agtataacag      720

ttggtacacg tttggaggtg ggaccaagct ggaaatgaaa cgggctgatg cggccgcata      780

gaagcttggc ggc                                                         793


<210>  34
<211>  604
<212>  DNA
<213>  Vibrio cholerae

<400>  34
ggtctcccca tgaaaaatga tgataagtta tatcgcgcag attctcgtcc gcctgatgaa       60

attaaacaga gcggtggtct gatgcctcgt ggtcagagcg aatattttga tcgtggcacc      120

cagatgaaca tcaacctgta tgatcatgca cgtggtacac agaccggttt tgttcgtcat      180

gatgatggtt atgttagcac cagcattagc ctgcgtagcg cacatctggt tggtcagacc      240

attctgagcg gtcatagcac ctattatatc tatgttattg caaccgcacc gaatatgttt      300

aacgttaatg atgttctggg tgcctatagt ccgcatccgg atgaacaaga ggttagcgca      360

ctgggtggta ttccgtatag ccagatttat ggttggtatc gtgttcattt tggtgtgctg      420

gatgaacagc tgcatcgtaa tcgtggttat cgtgatcgtt attatagcaa cctggatatt      480

gcaccggcag cagatggtta tggtctggca ggttttccgc ctgaacatcg tgcatggcgt      540

gaagaaccgt ggattcatca tgcaccgcct ggtgcaggta atgcaccgcg tagctctcga      600

gacc                                                                   604


<210>  35
<211>  444
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  3M2e-3Ea-SIIN-p323

<400>  35
ggtctccctc tagcctgctg accgaagttg aaaccccgat tcgtaatgaa tggggtagcc       60

gtagcaatga tagcagtctg ttaacggaag tggaaacacc gatccgcaac gagtggggta      120

gtcgttcaaa tgatagctca ctgctgacag aggtggaaac gcctataaga aacgaatggg      180

gttcacgtag taatgattca agcgcaagct ttgaagcaca gggtgcactg gcaaatattg      240

cagttgataa agcagccagt tttgaggccc aaggtgccct ggccaacatt gccgtggaca      300

aagcagcgtc attcgaggcg cagggtgcat tagccaatat cgccgtggat aaggcaagca      360

ttatcaactt tgagaaactg aaaattagcc aggcagttca tgcagcacat gccgaaatta      420

atgaagcagg tcgagcacga gacc                                             444


<210>  36
<211>  836
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CD103-binding scFv with FLAG and His tag

<400>  36
ggtctccagc aagtccgggt gaagttcagc tggttgaaag cggtggtggt ctggttcagc       60

ctggtcgtag cctgaaactg agctgtgcag caagcggttt tacctttagc gattattaca      120

tggcatgggt tcgtcagagc ccgaaaaaag gtctggaatg ggttgcaagc attagctatg      180

aaggcaccga tacctattat ggtgatagcg ttaaaggtcg ctttaccatt agccgtgata      240

atgcaaaaag caccctgtac ctgcagatga atagcctgcg tagcgaagat accgcaacct      300

attattgtgc aagcctgaat ggttataact accgctattt tgatttttgg ggtccgggta      360

caatggttac cgttagctca ggtggtggtg gtagcggtgg cggtggttct ggtggtggcg      420

gatcaggtgg cggtagcaat attcagatga cccagagtcc gagcattctg agcgcaagcg      480

ttggtgatcg tgttaccctg agctgtaaag caggtcagaa tattaacaat ttcctggcat      540

ggtatcagca gaaactgggt gaagcaccga aactgctgat ttataacgca aatagcctgg      600

aaaccggtat tccgagccgt tttagcggta gcggttctgg caccgattat accctgacca      660

ttagcagcct gcgtccggaa gatgttgcca cctatttttg tcagcagtat aatagctggt      720

atacctttgg tgccggtaca aaactggaac tgaaacgtgc agatgcagca gccaaactgg      780

attataaaga tgatgatgat aagcaccatc atcaccacca ctaataagcc gagacc          836


<210>  37
<211>  4243
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  E. coli expression vector

<400>  37
ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc       60

gcaacgcaat taatgtgagt tagcgcgaat tgatctggtt tgacagctta tcatcgactg      120

cacggtgcac caatgcttct ggcgtcaggc agccatcgga agctgtggta tggctgtgca      180

ggtcgtaaat cactgcataa ttcgtgtcgc tcaaggcgca ctcccgttct ggataatgtt      240

ttttgcgccg acatcataac ggttctggca aatattctga aatgagctgt tgacaattaa      300

tcatccggct cgtataatgt gtggaattgt gagcggataa caatttcaca caggaaacag      360

accatggaga cctaataact gcagtaataa ggtctctaag cttggctgtt ttggcggatg      420

agagaagatt ttcagcctga tacagattaa atcagaacgc agaagcggtc tgataaaaca      480

gaatttgcct ggcggcagta gcgcggtggt cccacctgac cccatgccga actcagaagt      540

gaaacgccgt agcgccgatg gtagtgtggg atctccccat gcgagagtag ggaactgcca      600

ggcatcaaat aaaacgaaag gctcagtcga aagactgggc ctttcgtttt atctgttgtt      660

tgtcggtgaa cgctctcctg agtaggacaa atccgccggg agcggatttg aacgttgcga      720

agcaacggcc cggagggtgg cgggcaggac gcccgccata aactgccagg catcaaatta      780

agcagaaggc catcctgacg gatggccttt ttgcgtttct acaaactctt tttgtttatt      840

tttctaaata cattcaaata tgtatccgct catggtgtgt ctcaaaatct ctgatgttac      900

attgcacaag ataaaaatat atcatcatga acaataaaac tgtctgctta cataaacagt      960

aatacaaggg gtgttatgag ccatattcaa cgggaaacgt cttgctcgag gccgcgatta     1020

aattccaaca tggatgctga tttatatggg tataaatggg ctcgcgataa tgtcgggcaa     1080

tcaggtgcga caatctatcg attgtatggg aagcccgatg cgccagagtt gtttctgaaa     1140

catggcaaag gtagcgttgc caatgatgtt acagatgaga tggtcagact aaactggctg     1200

acggaattta tgcctcttcc gaccatcaag cattttatcc gtactcctga tgatgcatgg     1260

ttactcacca ctgcgatccc cgggaaaaca gcattccagg tattagaaga atatcctgat     1320

tcaggtgaaa atattgttga tgcgctggca gtgttcctgc gccggttgca ttcgattcct     1380

gtttgtaatt gtccttttaa cagcgatcgc gtatttcgtc tcgctcaggc gcaatcacga     1440

atgaataacg gtttggttga tgcgagtgat tttgatgacg agcgtaatgg ctggcctgtt     1500

gaacaagtct ggaaagaaat gcataagctt ttgccattct caccggattc agtcgtcact     1560

catggtgatt tctcacttga taaccttatt tttgacgagg ggaaattaat aggttgtatt     1620

gatgttggac gagtcggaat cgcagaccga taccaggatc ttgccatcct atggaactgc     1680

ctcggtgagt tttctccttc attacagaaa cggctttttc aaaaatatgg tattgataat     1740

cctgatatga ataaattgca gtttcatttg atgctcgatg agtttttcta atcagaattg     1800

gttaattggt tgtaacactg gcagagcatt acgctgactt gacgggacgg cggctttgtt     1860

gaataaatcg aacttttgct gagttgaagg atcagatcac gcatcttccc gacaacgcag     1920

accgttccgt ggcaaagcaa aagttcaaaa tcaccaactg gtccccatga ccaaaatccc     1980

ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc     2040

ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc     2100

agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt     2160

cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt     2220

caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc     2280

tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa     2340

ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac     2400

ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg     2460

gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga     2520

gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact     2580

tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa     2640

cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc     2700

gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg     2760

ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat     2820

gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatggt gcactctcag     2880

tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac     2940

tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt     3000

ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag     3060

aggttttcac cgtcatcacc gaaacgcgcg aggcagcaga tcaattcgcg cgcgaaggcg     3120

aagcggcatg catttacgtt gacaccatcg aatggtgcaa aacctttcgc ggtatggcat     3180

gatagcgccc ggaagagagt caattcaggg tggtgaatgt gaaaccagta acgttatacg     3240

atgtcgcaga gtatgccggt gtctcttatc agaccgtttc ccgcgtggtg aaccaggcca     3300

gccacgtttc tgcgaaaacg cgggaaaaag tggaagcggc gatggcggag ctgaattaca     3360

ttcccaaccg cgtggcacaa caactggcgg gcaaacagtc gttgctgatt ggcgttgcca     3420

cctccagtct ggccctgcac gcgccgtcgc aaattgtcgc ggcgattaaa tctcgcgccg     3480

atcaactggg tgccagcgtg gtggtgtcga tggtagaacg aagcggcgtc gaagcctgta     3540

aagcggcggt gcacaatctt ctcgcgcaac gcgtcagtgg gctgatcatt aactatccgc     3600

tggatgacca ggatgccatt gctgtggaag ctgcctgcac taatgttccg gcgttatttc     3660

ttgatgtctc tgaccagaca cccatcaaca gtattatttt ctcccatgaa gacggtacgc     3720

gactgggcgt ggagcatctg gtcgcattgg gtcaccagca aatcgcgctg ttagcgggcc     3780

cattaagttc tgtctcggcg cgtctgcgtc tggctggctg gcataaatat ctcactcgca     3840

atcaaattca gccgatagcg gaacgggaag gcgactggag tgccatgtcc ggttttcaac     3900

aaaccatgca aatgctgaat gagggcatcg ttcccactgc gatgctggtt gccaacgatc     3960

agatggcgct gggcgcaatg cgcgccatta ccgagtccgg gctgcgcgtt ggtgcggata     4020

tctcggtagt gggatacgac gataccgaag acagctcatg ttatatcccg ccgtcaacca     4080

ccatcaaaca ggattttcgc ctgctggggc aaaccagcgt ggaccgcttg ctgcaactct     4140

ctcagggcca ggcggtgaag ggcaatcagc tgttgcccgt ctcactggtg aaaagaaaaa     4200

ccaccctggc gcccaatacg caaaccgcct ctccccgcgc gtt                       4243


<210>  38
<211>  1519
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CTA1-SIINFEKL(I)-P232(II)-CD103 scFv-FLAG tag-His Tag (

<400>  38
atcacctgca taccatggat gataagttat atcgggcaga ttctagacct cctgatgaaa       60

taaagcagtc aggtggtctt atgccaagag gacagagtga gtactttgac cgaggtactc      120

aaatgaatat caacctttat gatcatgcaa gaggaactca gacgggattt gttaggcacg      180

atgatggata tgtttccacc tcaattagtt tgagaagtgc ccacttagtg ggtcaaacta      240

tattgtctgg tcattctact tattatatat atgttatagc cactgcaccc aacatgttta      300

acgttaatga tgtattaggg gcatacagtc ctcatccaga tgaacaagaa gtttctgctt      360

taggtgggat tccatactcc caaatatatg gatggtatcg agttcatttt ggggtgcttg      420

atgaacaatt acatcgtaat aggggctaca gagatagata ttacagtaac ttagatattg      480

ctccagcagc agatggttat ggattggcag gtttccctcc ggagcataga gcttggaggg      540

aagagccgtg gattcatcat gcaccgccgg gttgtgggaa tgctccaaga tcatcgggat      600

ctactagtag tataatcaac tttgaaaaac tgatctccca ggctgttcac gctgctcacg      660

ctgaaatcaa cgaagctggt cgtgccagat ctcccgggga ggtgcagctg gtggagtctg      720

ggggaggctt agtgcagcct ggaaggtccc tgaaactctc ctgtgcagcc tcaggattca      780

ctttcagtga ctattacatg gcctgggtcc gccagtctcc aaagaagggt ctggagtggg      840

tcgcatccat tagttatgag ggtactgaca cttactatgg agactccgtg aagggccgat      900

tcactatctc cagagataat gcaaaaagca ccctgtacct gcaaatgaac agtctgaggt      960

ctgaggacac ggccacttat tattgtgcaa gcttgaatgg ctataactat cgatactttg     1020

acttctgggg cccaggaacc atggtcaccg tgtcctcagg tggaggcggt tcaggcggag     1080

gtggctctgg cggtggaggg agcggtggag ggagtaacat ccagatgacc cagtctcctt     1140

caatactgtc tgcatctgtg ggagacagag tcactctcag ctgcaaagca ggtcagaata     1200

ttaacaattt cttagcctgg tatcagcaaa agcttggaga agctcccaaa ctcctgatat     1260

ataatgcaaa cagtttggaa acgggcatcc catcaaggtt cagtggcagt ggatctggca     1320

cagattacac actcaccatc agcagcctgc ggcctgaaga tgttgccaca tatttctgcc     1380

agcagtataa cagttggtac acgtttggag ctgggaccaa gctggaactg aaacgggctg     1440

atgcggccgc aaagcttgat tacaaggatg acgatgacaa gcatcaccat catcaccatt     1500

agagcttcgc aggtgcttc                                                  1519


<210>  39
<211>  1499
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CTA1-P232(II)-CD103 scFv-FLAG tag-His Tag

<400>  39
atcacctgca taccatggat ggatgataag ttatataagg cagattctag acctcctgat       60

gaaataaagc agtcaggtgg tcttatgcca agaggacaga gtgagtactt tgaccgaggt      120

actcaaatga atatcaacct ttatgatcat gcaagaggaa ctcagacggg atttgttagg      180

cacgatgatg gatatgtttc cacctcaatt agtttgagaa gtgcccactt agtgggtcaa      240

actatattgt ctggtcattc tacttattat atatatgtta tagccactgc acccaacatg      300

tttaacgtta atgatgtatt aggggcatac agtcctcatc cagatgaaca agaagtttct      360

gctttaggtg ggattccata ctcccaaata tatggatggt atcgagttca ttttggggtg      420

cttgatgaac aattacatcg taataggggc tacagagata gatattacag taacttagat      480

attgctccag cagcagatgg ttatggattg gcaggtttcc ctccggagca tagagcttgg      540

agggaagagc cgtggattca tcatgcaccg ccgggttgtg ggaatgctcc aagatcatcg      600

ggatctacta gtatctccca ggctgttcac gctgctcacg ctgaaatcaa cgaagctggt      660

cgtgccagat ctcccgggga ggtgcagctg gtggagtctg ggggaggctt agtgcagcct      720

ggaaggtccc tgaaactctc ctgtgcagcc tcaggattca ctttcagtga ctattacatg      780

gcctgggtcc gccagtctcc aaagaagggt ctggagtggg tcgcatccat tagttatgag      840

ggtactgaca cttactatgg agactccgtg aagggccgat tcactatctc cagagataat      900

gcaaaaagca ccctgtacct gcaaatgaac agtctgaggt ctgaggacac ggccacttat      960

tattgtgcaa gcttgaatgg ctataactat cgatactttg acttctgggg cccaggaacc     1020

atggtcaccg tgtcctcagg tggaggcggt tcaggcggag gtggctctgg cggtggaggg     1080

agcggtggag ggagtaacat ccagatgacc cagtctcctt caatactgtc tgcatctgtg     1140

ggagacagag tcactctcag ctgcaaagca ggtcagaata ttaacaattt tttagcctgg     1200

tatcagcaaa agcttggaga agctcccaaa ctcctgatat ataatgcaaa cagtttggaa     1260

acgggcatcc catcaaggtt cagtggcagt ggatttggca cagattacac actcaccatc     1320

agcagcctgc ggcctgaaga tgttgccaca tatttttgcc agcagtataa cagttggtac     1380

acgtttggag gtgggaccaa gctggaaatg aaacgggctg atgcggccgc aaagcttgat     1440

tacaaggatg acgatgacaa gcatcaccat catcaccatt agagcttcgc aggtgcttc      1499


<210>  40
<211>  1499
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CTA1(R7k)-P323- CD103 scFv-FLAG tag-His Tag

<400>  40
atcacctgca taccatggat ggatgataag ttatataagg cagattctag acctcctgat       60

gaaataaagc agtcaggtgg tcttatgcca agaggacaga gtgagtactt tgaccgaggt      120

actcaaatga atatcaacct ttatgatcat gcaagaggaa ctcagacggg atttgttagg      180

cacgatgatg gatatgtttc cacctcaatt agtttgagaa gtgcccactt agtgggtcaa      240

actatattgt ctggtcattc tacttattat atatatgtta tagccactgc acccaacatg      300

tttaacgtta atgatgtatt aggggcatac agtcctcatc cagatgaaca agaagtttct      360

gctttaggtg ggattccata ctcccaaata tatggatggt atcgagttca ttttggggtg      420

cttgatgaac aattacatcg taataggggc tacagagata gatattacag taacttagat      480

attgctccag cagcagatgg ttatggattg gcaggtttcc ctccggagca tagagcttgg      540

agggaagagc cgtggattca tcatgcaccg ccgggttgtg ggaatgctcc aagatcatcg      600

ggatctacta gtatctccca ggctgttcac gctgctcacg ctgaaatcaa cgaagctggt      660

cgtgccagat ctcccgggga ggtgcagctg gtggagtctg ggggaggctt agtgcagcct      720

ggaaggtccc tgaaactctc ctgtgcagcc tcaggattca ctttcagtga ctattacatg      780

gcctgggtcc gccagtctcc aaagaagggt ctggagtggg tcgcatccat tagttatgag      840

ggtactgaca cttactatgg agactccgtg aagggccgat tcactatctc cagagataat      900

gcaaaaagca ccctgtacct gcaaatgaac agtctgaggt ctgaggacac ggccacttat      960

tattgtgcaa gcttgaatgg ctataactat cgatactttg acttctgggg cccaggaacc     1020

atggtcaccg tgtcctcagg tggaggcggt tcaggcggag gtggctctgg cggtggaggg     1080

agcggtggag ggagtaacat ccagatgacc cagtctcctt caatactgtc tgcatctgtg     1140

ggagacagag tcactctcag ctgcaaagca ggtcagaata ttaacaattt tttagcctgg     1200

tatcagcaaa agcttggaga agctcccaaa ctcctgatat ataatgcaaa cagtttggaa     1260

acgggcatcc catcaaggtt cagtggcagt ggatttggca cagattacac actcaccatc     1320

agcagcctgc ggcctgaaga tgttgccaca tatttttgcc agcagtataa cagttggtac     1380

acgtttggag gtgggaccaa gctggaaatg aaacgggctg atgcggccgc aaagcttgat     1440

tacaaggatg acgatgacaa gcatcaccat catcaccatt agagcttcgc aggtgcttc      1499


<210>  41
<211>  4151
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pSY-AaRI

<400>  41
ctccaaaagg agcctttaat tgtatcggtt tatcagcttg ctttcgaggt gaatttcgac       60

ctctagacca cgcttggctg caggtcgacg gatctcgatc ccgcgaaatt aatacgactc      120

actataggga gaccacaacg gtttccctct agaaataatt ttgtttaact ttaagaagga      180

gatataccat ggatggcagg tgctataagt taatcatcac cacctgccat tagagcttct      240

caaataagat ggtcccatag tctgtatcca aataatgaat cttcgggtgt ttccctttag      300

ctaagcacag atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc      360

gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg      420

ctgaaaggag gaactatatc cggatgggaa ttccccgcgc gcgatgccct ttcgtcttcg      480

aataaatacc tgtgacggaa gatcacttcg cagaataaat aaatcctggt gtccctgttg      540

ataccgggaa gccctgggcc aacttttggc gaaaatgaga cgttgatcgg cacgtaagag      600

gttccaactt tcaccataat gaaataagat cactaccggg cgtatttttt gagttatcga      660

gattttcagg agctaaggaa gctaaaatgg agaaaaaaat cactggatat accaccgttg      720

atatatccca atggcatcgt aaagaacatt ttgaggcatt tcagtcagtt gctcaatgta      780

cctataacca gaccgttcag ctggatatta cggccttttt aaagaccgta aagaaaaata      840

agcacaagtt ttatccggcc tttattcaca ttcttgcccg cctgatgaat gctcatccgg      900

aatttcgtat ggcaatgaaa gacggtgagc tggtgatatg ggatagtgtt cacccttgtt      960

acaccgtttt ccatgagcaa actgaaacgt tttcatcgct ctggagtgaa taccacgacg     1020

atttccggca gtttctacac atatattcgc aagatgtggc gtgttacggt gaaaacctgg     1080

cctatttccc taaagggttt attgagaata tgtttttcgt ctcagccaat ccctgggtga     1140

gtttcaccag ttttgattta aacgtggcga atatggacaa cttcttcgcc cccgttttca     1200

ctatgggcaa atattatacg caaggcgaca aggtgctgat gccgctggcg attcaggttc     1260

atcatgccgt ttgtgatggc ttccatgtcg gcagaatgct taatgaatta caacagtact     1320

gcgatgagtg gcagggcggg gcgtaatttt tttaaggcag ttattggtgc ccttaaacgc     1380

ctggttgcta cgcctgaata agtgataata agcggatgaa tggcagaaat tcgagcccgc     1440

ctaatgagcg ggcttttttt tagcccgcct aatgagcggg cttttttttc gaaagcaaat     1500

tcgacccatc gcgcgcgggg agtcaactca gcaaaagttc gatttattca acaaagccac     1560

gttgtgtctc aaaatctctg atgttacatt gcacaagata aaaatatatc atcatgaaca     1620

ataaaactgt ctgcttacat aatattgaaa aaggaagagt atgagtattc aacatttccg     1680

tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac     1740

gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact     1800

ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat     1860

gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga     1920

gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac     1980

agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat     2040

gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac     2100

cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct     2160

gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac     2220

gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga     2280

ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg     2340

gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact     2400

ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac     2460

tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta     2520

actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt     2580

taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga     2640

gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc     2700

tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt     2760

ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc     2820

gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc     2880

tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg     2940

cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg     3000

gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga     3060

actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc     3120

ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg     3180

gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg     3240

atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt     3300

tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc     3360

tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg     3420

aacgaccgag cgcagatcaa attgtaaacg ttaatatttt gttaaaattc gcgttaaatt     3480

tttgttaaat cagctcattt tttaaccaat aggccgaaat cggcaaaatc ccttataaat     3540

caaaagaata gaccgagata gggttgagtg ttgttccagt ttggaacaag agtccactat     3600

taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt ctatcagggc gatggcccac     3660

tacgtgaacc atcaccctaa tcaagttttt tggggtcgag gtgccgtaaa gcactaaatc     3720

ggaaccctaa agggagcccc cgatttagag cttgacgggg aaagccggcg aacgtggcga     3780

gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc gctggcaagt gtagcggtca     3840

cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc gctacagggc gcgtgatctg     3900

catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac     3960

cgtcatcacc gaaacgcgcg aggcagagct tggaattgcg aataataatt ttttcacgtt     4020

gaaaatctcc aaaaaaaaag gctccaaaag gagcctttaa ttgtatcggt ttatcagctt     4080

gctttcgagg tgaattagct tggaattgcg aataataatt ttttcacgtt gaaaatctcc     4140

aaaaaaaaag g                                                          4151


<210>  42
<211>  1887
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  mSS-CTA1-3(M2e)-3(Ea)-CD103 scFv- FLAG tag- His Tag

<400>  42
gcgcggatcc atgaaatttt tggtgaacgt ggccttggtg tttatggtgg tgtacatttc       60

gtacatttac gccgacccgt cgccggacga caaattgtac agagccgact cgagaccgcc      120

ggacgaaatt aaacaatcgg gcggcttgat gccgagaggc caatcggaat actttgacag      180

aggcacgcaa atgaacatta acttgtacga ccacgccaga ggcacgcaaa cgggctttgt      240

gagacacgac gacggctacg tgtcgacgtc gatttcgttg agatcggccc acttggtggg      300

ccaaacgatt ttgtcgggcc actcgacgta ctacatttac gtgattgcca cggccccgaa      360

catgtttaac gtgaacgacg tgttgggcgc ctactcgccg cacccggacg aacaagaagt      420

gtcggccttg ggcggcattc cgtactcgca aatttacggc tggtacagag tgcactttgg      480

cgtgttggac gaacaattgc acagaaacag aggctacaga gacagatact actcgaactt      540

ggacattgcc ccggccgccg acggctacgg cttggccggc tttccgccgg aacacagagc      600

ctggagagaa gaaccgtgga ttcaccacgc cccgccgggc tgtggcaacg ccccgagatc      660

gtcggggaat tcgttgttga cggaagtgga aacgccgatt agaaacgaat ggggctcgag      720

atcgaacgac tcgtcgttgt tgacggaagt ggaaacgccg attagaaacg aatggggctc      780

gagatcgaac gactcgtcgt tgttgacgga agtggaaacg ccgattagaa acgaatgggg      840

ctcgagatcg aacgactcgt cggacggttt aattaagttt aaaccatatg aagccagctt      900

cgaggcccag ggcgccctgg ccaacatcgc cgtggacaag gccgccagct tcgaggccca      960

gggcgccctg gccaacatcg ccgtggacaa ggccgccagc ttcgaggccc agggcgccct     1020

ggccaacatc gccgtggaca aggccagttt aaaccccggg agcggggagg tgcagctggt     1080

ggagtctggg ggaggcttag tgcagcctgg aaggtccctg aaactctcct gtgcagcctc     1140

aggattcact ttcagtgact attacatggc ctgggtccgc cagtctccaa agaagggtct     1200

ggagtgggtc gcatccatta gttatgaggg tactgacact tactatggag actccgtgaa     1260

gggccgattc actatctcca gagataatgc aaaaagcacc ctgtacctgc aaatgaacag     1320

tctgaggtct gaggacacgg ccacttatta ttgtgcaagc ttgaatggct ataactatcg     1380

atactttgac ttctggggcc caggaaccat ggtcaccgtg tcctcaggtg gaggcggttc     1440

aggcggaggt ggctctggcg gtggagggag cggtggaggg agtaacatcc agatgaccca     1500

gtctccttca atactgtctg catctgtggg agacagagtc actctcagct gcaaagcagg     1560

tcagaatatt aacaattttt tagcctggta tcagcaaaag cttggagaag ctcccaaact     1620

cctgatatat aatgcaaaca gtttggaaac gggcatccca tcaaggttca gtggcagtgg     1680

atttggcaca gattacacac tcaccatcag cagcctgcgg cctgaagatg ttgccacata     1740

tttttgccag cagtataaca gttggtacac gtttggaggt gggaccaagc tggaaatgaa     1800

acgggctgat gcggccgcat attcgaagag ctccgactac aaagacgacg acgacaaaca     1860

ccaccaccac caccactagg gtaccaa                                         1887


<210>  43
<211>  601
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CTA1 - 3M2e - 3Ea - CD103-binding scFv - FLAG tag - His tag

<400>  43

Asp Pro Ser Pro Asp Asp Lys Leu Tyr Arg Ala Asp Ser Arg Pro Pro 
1               5                   10                  15      


Asp Glu Ile Lys Gln Ser Gly Gly Leu Met Pro Arg Gly Gln Ser Glu 
            20                  25                  30          


Tyr Phe Asp Arg Gly Thr Gln Met Asn Ile Asn Leu Tyr Asp His Ala 
        35                  40                  45              


Arg Gly Thr Gln Thr Gly Phe Val Arg His Asp Asp Gly Tyr Val Ser 
    50                  55                  60                  


Thr Ser Ile Ser Leu Arg Ser Ala His Leu Val Gly Gln Thr Ile Leu 
65                  70                  75                  80  


Ser Gly His Ser Thr Tyr Tyr Ile Tyr Val Ile Ala Thr Ala Pro Asn 
                85                  90                  95      


Met Phe Asn Val Asn Asp Val Leu Gly Ala Tyr Ser Pro His Pro Asp 
            100                 105                 110         


Glu Gln Glu Val Ser Ala Leu Gly Gly Ile Pro Tyr Ser Gln Ile Tyr 
        115                 120                 125             


Gly Trp Tyr Arg Val His Phe Gly Val Leu Asp Glu Gln Leu His Arg 
    130                 135                 140                 


Asn Arg Gly Tyr Arg Asp Arg Tyr Tyr Ser Asn Leu Asp Ile Ala Pro 
145                 150                 155                 160 


Ala Ala Asp Gly Tyr Gly Leu Ala Gly Phe Pro Pro Glu His Arg Ala 
                165                 170                 175     


Trp Arg Glu Glu Pro Trp Ile His His Ala Pro Pro Gly Cys Gly Asn 
            180                 185                 190         


Ala Pro Arg Ser Ser Gly Asn Ser Leu Leu Thr Glu Val Glu Thr Pro 
        195                 200                 205             


Ile Arg Asn Glu Trp Gly Ser Arg Ser Asn Asp Ser Ser Leu Leu Thr 
    210                 215                 220                 


Glu Val Glu Thr Pro Ile Arg Asn Glu Trp Gly Ser Arg Ser Asn Asp 
225                 230                 235                 240 


Ser Ser Leu Leu Thr Glu Val Glu Thr Pro Ile Arg Asn Glu Trp Gly 
                245                 250                 255     


Ser Arg Ser Asn Asp Ser Ser Asp Gly Leu Ile Lys Phe Lys Pro Tyr 
            260                 265                 270         


Glu Ala Ser Phe Glu Ala Gln Gly Ala Leu Ala Asn Ile Ala Val Asp 
        275                 280                 285             


Lys Ala Ala Ser Phe Glu Ala Gln Gly Ala Leu Ala Asn Ile Ala Val 
    290                 295                 300                 


Asp Lys Ala Ala Ser Phe Glu Ala Gln Gly Ala Leu Ala Asn Ile Ala 
305                 310                 315                 320 


Val Asp Lys Ala Ser Leu Asn Pro Gly Ser Gly Glu Val Gln Leu Val 
                325                 330                 335     


Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Arg Ser Leu Lys Leu Ser 
            340                 345                 350         


Cys Ala Ala Ser Gly Phe Thr Phe Ser Asp Tyr Tyr Met Ala Trp Val 
        355                 360                 365             


Arg Gln Ser Pro Lys Lys Gly Leu Glu Trp Val Ala Ser Ile Ser Tyr 
    370                 375                 380                 


Glu Gly Thr Asp Thr Tyr Tyr Gly Asp Ser Val Lys Gly Arg Phe Thr 
385                 390                 395                 400 


Ile Ser Arg Asp Asn Ala Lys Ser Thr Leu Tyr Leu Gln Met Asn Ser 
                405                 410                 415     


Leu Arg Ser Glu Asp Thr Ala Thr Tyr Tyr Cys Ala Ser Leu Asn Gly 
            420                 425                 430         


Tyr Asn Tyr Arg Tyr Phe Asp Phe Trp Gly Pro Gly Thr Met Val Thr 
        435                 440                 445             


Val Ser Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly 
    450                 455                 460                 


Gly Ser Gly Gly Gly Ser Asn Ile Gln Met Thr Gln Ser Pro Ser Ile 
465                 470                 475                 480 


Leu Ser Ala Ser Val Gly Asp Arg Val Thr Leu Ser Cys Lys Ala Gly 
                485                 490                 495     


Gln Asn Ile Asn Asn Phe Leu Ala Trp Tyr Gln Gln Lys Leu Gly Glu 
            500                 505                 510         


Ala Pro Lys Leu Leu Ile Tyr Asn Ala Asn Ser Leu Glu Thr Gly Ile 
        515                 520                 525             


Pro Ser Arg Phe Ser Gly Ser Gly Phe Gly Thr Asp Tyr Thr Leu Thr 
    530                 535                 540                 


Ile Ser Ser Leu Arg Pro Glu Asp Val Ala Thr Tyr Phe Cys Gln Gln 
545                 550                 555                 560 


Tyr Asn Ser Trp Tyr Thr Phe Gly Gly Gly Thr Lys Leu Glu Met Lys 
                565                 570                 575     


Arg Ala Asp Ala Ala Ala Tyr Ser Lys Ser Ser Asp Tyr Lys Asp Asp 
            580                 585                 590         


Asp Asp Lys His His His His His His 
        595                 600     


