<110>    THE ASAN FOUNDATION
         University of Ulsan Foundation For Industry Cooperation

<120>    Discovery of novel Akkermansia muciniphila AK32 strain and
         application for preventing or treating intestinal injury

<130>    MPCT21-036

<150>    KR 10-2020-0056034
<151>    2020-05-11

<160>    25

<170>    KoPatentIn 3.0

<210>    1
<211>    20
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    PCR primer


<400>    1
cagcacgtga aggtggggac                                                     20


<210>    2
<211>    20
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    PCR primer


<400>    2
ccttgcggtt ggcttcagat                                                     20


<210>    3
<211>    553
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    AK32 MMD (Na+  translocating methymalonyl-CoA/oxaloacetate
         decarboxylase)


<400>    3
Met Ser Leu Phe Asp Ser Leu Ile Thr Phe Leu Gln Gly Met Gly Val
  1               5                  10                  15 

Phe Ser Leu Ser Trp Gln Met Val Gly Met Trp Gly Ile Ala Ile Leu
             20                  25                  30 

Leu Leu Tyr Leu Gly Val Ala Lys Gln Tyr Glu Pro Leu Leu Met Val
         35                  40                  45 

Pro Ile Ala Phe Gly Ala Leu Ile Ala Asn Ile Pro Asp Asn Gly Met
     50                  55                  60 

Leu Ile Thr Gln Leu Asn Gln Gln Val Ile Ser Ser Asn Glu Gln Gly
 65                  70                  75                  80 

Glu Val Thr Ser Thr Ser Leu Asn Asn Val Gly Tyr Leu Arg Val His
                 85                  90                  95 

Val Ala Pro Leu Gln Gln Thr Pro Ala Lys Val Pro Ala Asn Leu Thr
            100                 105                 110 

Thr Pro Glu Ala Arg Ala Gln Tyr Leu Glu Thr Met Gln Gln Pro Met
        115                 120                 125 

Gln Val Tyr Pro Gly Ser Gln Leu Thr Val Ser Lys Ile Lys Ser Val
    130                 135                 140 

Arg Glu Ser Gln Glu Lys Ala Lys Ala Asp Ala Ala Arg Leu Gly Asp
145                 150                 155                 160 

Asp Ser Leu Thr Val Asp Pro Asn Leu Lys Asp Phe Gln Asn Val Glu
                165                 170                 175 

Asp Asn Gly Asn Glu Pro Val Phe Leu Leu Thr Asn Gly Glu Gly Thr
            180                 185                 190 

Thr Val Val Arg Gln Gln Gly Val Asn Tyr Phe Asp Thr Ser Gly Asn
        195                 200                 205 

Arg Val Pro Val Asp Leu Lys Thr Gln Lys Leu Glu Pro Leu Val Val
    210                 215                 220 

Ser Ala Ala Gly Lys Tyr Val Ala Val Gly Gln His Thr Gln Glu Leu
225                 230                 235                 240 

Leu Val Thr Ser Ile His Gly Gly Leu Tyr Asp Trp Ile Gly Leu Gly
                245                 250                 255 

Ile Lys Ala Glu Ile Phe Pro Pro Ile Ile Phe Leu Gly Val Gly Ala
            260                 265                 270 

Leu Thr Asp Phe Gly Pro Leu Leu Ala Ala Pro Arg Thr Leu Leu Leu
        275                 280                 285 

Gly Ala Ala Ala Gln Val Gly Val Ala Ala Thr Phe Phe Met Ala Leu
    290                 295                 300 

Phe Met Gly Phe Asn Pro Asn Glu Ala Ala Ser Ile Gly Ile Ile Gly
305                 310                 315                 320 

Gly Ala Asp Gly Pro Thr Ser Ile Phe Leu Thr Met Lys Leu Ala Pro
                325                 330                 335 

His Leu Leu Gly Ala Val Ala Val Ala Ala Tyr Thr Tyr Met Ser Leu
            340                 345                 350 

Val Pro Leu Ile Gln Pro Pro Ile Met Ala Leu Leu Thr Thr Lys Lys
        355                 360                 365 

Glu Arg Leu Ile Arg Met Lys Ser Leu Arg Thr Val Ser Lys Ser Glu
    370                 375                 380 

Lys Leu Phe Phe Ala Val Leu Val Thr Ile Val Thr Ile Leu Leu Ile
385                 390                 395                 400 

Pro Asp Ala Ser Pro Leu Ile Gly Met Leu Met Leu Gly Asn Phe Leu
                405                 410                 415 

Arg Glu Cys Lys Val Thr Glu Arg Leu Val Gln Ala Ser Gln Asn Glu
            420                 425                 430 

Ile Ile Asn Ile Val Thr Ile Phe Leu Gly Thr Ser Val Gly Leu Thr
        435                 440                 445 

Met Gln Gly Asp Arg Phe Leu Gln Ala Glu Thr Leu Leu Ile Ile Leu
    450                 455                 460 

Leu Gly Ile Val Ala Phe Gly Val Ala Thr Ala Gly Gly Val Ile Ala
465                 470                 475                 480 

Ala Lys Leu Met Asn Leu Ile Trp Arg Lys Asn Pro Val Asn Pro Leu
                485                 490                 495 

Ile Gly Ser Ala Gly Val Ser Ala Val Pro Met Ala Ala Arg Val Ser
            500                 505                 510 

His Asn Val Gly Gln Lys Tyr Asp Pro Ser Asn Tyr Leu Leu Met His
        515                 520                 525 

Ala Met Gly Pro Asn Val Ala Gly Val Ile Gly Thr Ala Val Ile Ala
    530                 535                 540 

Gly Tyr Tyr Ile Ala Thr Leu Ala Lys
545                 550            


<210>    4
<211>    20
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    Muc2 forward primer


<400>    4
ccttagccaa gggctcggaa                                                     20


<210>    5
<211>    20
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    Muc2 reverse primer


<400>    5
ggcccgagag tagaccttgg                                                     20


<210>    6
<211>    20
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    Lyz1 forward primer


<400>    6
atggcgaaca caatgtcaaa                                                     20


<210>    7
<211>    20
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    Lyz1 reverse primer


<400>    7
gccctgtttc tgctgaagtc                                                     20


<210>    8
<211>    20
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    Wnt3 forward primer


<400>    8
cttctaatgg agccccacct                                                     20


<210>    9
<211>    21
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    Wnt3 reverse primer


<400>    9
gaggccagag atgtgtactg c                                                   21


<210>    10
<211>    20
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    Axin2 forward primer


<400>    10
aacctatgcc cgtttcctct                                                     20


<210>    11
<211>    20
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    Axin2 reverse primer


<400>    11
gagtgtaaag acttggtcca                                                     20


<210>    12
<211>    20
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    Ctnnb1 forward primer


<400>    12
atggagccgg acagaaaagc                                                     20


<210>    13
<211>    20
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    Ctnnb1 reverse primer


<400>    13
tgggaggtgt caacatcttc                                                     20


<210>    14
<211>    20
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    Lgr5 forward primer


<400>    14
cctgtccagg ctttcagaag                                                     20


<210>    15
<211>    20
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    Lgr5 reverse primer


<400>    15
ctgtggagtc catcaaagca                                                     20


<210>    16
<211>    25
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    beta-actin forward primer


<400>    16
tggaatcctg tggcatccat gaaac                                               25


<210>    17
<211>    25
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    beta-actin reverse primer


<400>    17
taaaacgcag ctcagtaaca gtccg                                               25


<210>    18
<211>    20
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    pdh forward primer


<400>    18
aaccgattat tgaagcggca                                                     20


<210>    19
<211>    20
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    pdh reverse primer


<400>    19
atattggcgg cttcgtgaaa                                                     20


<210>    20
<211>    20
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    mmd forward primer


<400>    20
gaccaagaag gaacgcctca                                                     20


<210>    21
<211>    20
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    mmd reverse primer


<400>    21
gttccgtcac cttgcattcg                                                     20


<210>    22
<211>    20
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    Universal 16S rDNA forward primer


<400>    22
actcctacgg gaggcagcag                                                     20


<210>    23
<211>    17
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    Universal 16S rDNA reverse primer


<400>    23
attaccgcgg ctgctgg                                                        17


<210>    24
<211>    20
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    A. muciniphila 16S rDNA forward primer


<400>    24
cagcacgtga aggtggggac                                                     20


<210>    25
<211>    20
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    A. muciniphila 16S rDNA reverse primer


<400>    25
ccttgcggtt ggcttcagat                                                     20
