                         SEQUENCE LISTING

<110>  SEKKAL, Fayal </110>
 
<120>  SEQUENCES GENOMIQUES ET POLYPEPTIDIQUES DU SARS-COV-2, POUR UNE 
       DOUBLE UTILISATION VACCINALE ET DE TEST DE L'IMMUNITE CELLULAIRE 
       CUTANEE DU SARS-COV-2 </120>

<130>  BRV000063991 </130>

<140>  BRV000063991 </140>
<141>  2020-05-09

<160>  6     

<170>  PatentIn version 3.5

<210>  1
<211>  1623
<212>  DNA
<213>  SARS-CoV-2 </213>


<220>
<221>  CDS
<222>  (1)..(1623)
<223>  Domaine S2 protine S

<400>  1
caa tcc atc att gcc tac act atg tca ctt cgt gca gaa aat tca gtt         48
Gln Ser Ile Ile Ala Tyr Thr Met Ser Leu Arg Ala Glu Asn Ser Val           
1               5                   10                  15                

gct tac tct aat aac tct att gcc ata ccc aca aat ttt act att agt         96
Ala Tyr Ser Asn Asn Ser Ile Ala Ile Pro Thr Asn Phe Thr Ile Ser           
            20                  25                  30                    

gtt acc aca gaa att cta cca gtg tct atg acc aag aca tca gta gat        144
Val Thr Thr Glu Ile Leu Pro Val Ser Met Thr Lys Thr Ser Val Asp           
        35                  40                  45                        

tgt aca atg tac att tgt ggt gat tca act gaa tgc agc aat ctt ttg        192
Cys Thr Met Tyr Ile Cys Gly Asp Ser Thr Glu Cys Ser Asn Leu Leu           
    50                  55                  60                            

ttg caa tat ggc agt ttt tgt aca caa tta aac cgt gct tta act gga        240
Leu Gln Tyr Gly Ser Phe Cys Thr Gln Leu Asn Arg Ala Leu Thr Gly           
65                  70                  75                  80            

ata gct gtt gaa caa gac aaa aac acc caa gaa gtt ttt gca caa gtc        288
Ile Ala Val Glu Gln Asp Lys Asn Thr Gln Glu Val Phe Ala Gln Val           
                85                  90                  95                

aaa caa att tac aaa aca cca cca att aaa gat ttt ggt ggt ttt aat        336
Lys Gln Ile Tyr Lys Thr Pro Pro Ile Lys Asp Phe Gly Gly Phe Asn           
            100                 105                 110                   

ttt tca caa ata tta cca gat cca tca aaa cca agc aaa agg tca ttt        384
Phe Ser Gln Ile Leu Pro Asp Pro Ser Lys Pro Ser Lys Arg Ser Phe           
        115                 120                 125                       

att gaa gat cta ctt ttc aac aaa gtg aca ctt gca gat gct ggc ttc        432
Ile Glu Asp Leu Leu Phe Asn Lys Val Thr Leu Ala Asp Ala Gly Phe           
    130                 135                 140                           

atc aaa caa tat ggt gat tgc ctt ggt gat att gct gct aga gac ctc        480
Ile Lys Gln Tyr Gly Asp Cys Leu Gly Asp Ile Ala Ala Arg Asp Leu           
145                 150                 155                 160           

att tgt gca caa aag ttt aac ggc ctt act gtt ttg cca cct ttg ctc        528
Ile Cys Ala Gln Lys Phe Asn Gly Leu Thr Val Leu Pro Pro Leu Leu           
                165                 170                 175               

aca gat gaa atg att gct caa tac act tct gca ctg tta gcg ggt aca        576
Thr Asp Glu Met Ile Ala Gln Tyr Thr Ser Ala Leu Leu Ala Gly Thr           
            180                 185                 190                   

atc act tct ggt tgg acc ttt ggt gca ggt gct gca tta caa ata cca        624
Ile Thr Ser Gly Trp Thr Phe Gly Ala Gly Ala Ala Leu Gln Ile Pro           
        195                 200                 205                       

ttt gct atg caa atg gct tat agg ttt aat ggt att gga gtt aca cag        672
Phe Ala Met Gln Met Ala Tyr Arg Phe Asn Gly Ile Gly Val Thr Gln           
    210                 215                 220                           

aat gtt ctc tat gag aac caa aaa ttg att gcc aac caa ttt aat agt        720
Asn Val Leu Tyr Glu Asn Gln Lys Leu Ile Ala Asn Gln Phe Asn Ser           
225                 230                 235                 240           

gct att ggc aaa att caa gac tca ctt tct tcc aca gca agt gca ctt        768
Ala Ile Gly Lys Ile Gln Asp Ser Leu Ser Ser Thr Ala Ser Ala Leu           
                245                 250                 255               

gga aaa ctt caa gat gtg gtc aac caa aat gca caa gct tta aac acg        816
Gly Lys Leu Gln Asp Val Val Asn Gln Asn Ala Gln Ala Leu Asn Thr           
            260                 265                 270                   

ctt gtt aaa caa ctt agc tcc aat ttt ggt gca att tca agt gtt tta        864
Leu Val Lys Gln Leu Ser Ser Asn Phe Gly Ala Ile Ser Ser Val Leu           
        275                 280                 285                       

aat gat atc ctt tca cgt ctt gac aaa gtt gag gct gaa gtg caa att        912
Asn Asp Ile Leu Ser Arg Leu Asp Lys Val Glu Ala Glu Val Gln Ile           
    290                 295                 300                           

gat agg ttg atc aca ggc aga ctt caa agt ttg cag aca tat gtg act        960
Asp Arg Leu Ile Thr Gly Arg Leu Gln Ser Leu Gln Thr Tyr Val Thr           
305                 310                 315                 320           

caa caa tta att aga gct gca gaa atc aga gct tct gct aat ctt gct       1008
Gln Gln Leu Ile Arg Ala Ala Glu Ile Arg Ala Ser Ala Asn Leu Ala           
                325                 330                 335               

gct act aaa atg tca gag tgt gta ctt gga caa tca aaa aga gtt gat       1056
Ala Thr Lys Met Ser Glu Cys Val Leu Gly Gln Ser Lys Arg Val Asp           
            340                 345                 350                   

ttt ttg gga aag ggc tat cat ctt atg tcc ttc cct cag tca gca cct       1104
Phe Leu Gly Lys Gly Tyr His Leu Met Ser Phe Pro Gln Ser Ala Pro           
        355                 360                 365                       

cat ggt gta gtc ttc ttg cat gtg act tat gtc cct gca caa gaa aag       1152
His Gly Val Val Phe Leu His Val Thr Tyr Val Pro Ala Gln Glu Lys           
    370                 375                 380                           

aac ttc aca act gct cct gcc att tgt cat gat gga aaa gca cac ttt       1200
Asn Phe Thr Thr Ala Pro Ala Ile Cys His Asp Gly Lys Ala His Phe           
385                 390                 395                 400           

cct cgt gaa ggt gtc ttt gtt tca aat ggc aca cac tgg ttt gta aca       1248
Pro Arg Glu Gly Val Phe Val Ser Asn Gly Thr His Trp Phe Val Thr           
                405                 410                 415               

caa agg aat ttt tat gaa cca caa atc att act aca gac aac aca ttt       1296
Gln Arg Asn Phe Tyr Glu Pro Gln Ile Ile Thr Thr Asp Asn Thr Phe           
            420                 425                 430                   

gtg tct ggt aac tgt gat gtt gta ata gga att gtc aac aac aca gtt       1344
Val Ser Gly Asn Cys Asp Val Val Ile Gly Ile Val Asn Asn Thr Val           
        435                 440                 445                       

tat gat cct ttg caa cct gaa tta gac tca ttc aag gag gag tta gat       1392
Tyr Asp Pro Leu Gln Pro Glu Leu Asp Ser Phe Lys Glu Glu Leu Asp           
    450                 455                 460                           

aaa tat ttt aag aat cat aca tca cca gat gtt gat tta ggt gac atc       1440
Lys Tyr Phe Lys Asn His Thr Ser Pro Asp Val Asp Leu Gly Asp Ile           
465                 470                 475                 480           

tct ggc att aat gct tca gtt gta aac att caa aaa gaa att gac cgc       1488
Ser Gly Ile Asn Ala Ser Val Val Asn Ile Gln Lys Glu Ile Asp Arg           
                485                 490                 495               

ctc aat gag gtt gcc aag aat tta aat gaa tct ctc atc gat ctc caa       1536
Leu Asn Glu Val Ala Lys Asn Leu Asn Glu Ser Leu Ile Asp Leu Gln           
            500                 505                 510                   

gaa ctt gga aag tat gag cag tat ata aaa tgg cca tgg tac att tgg       1584
Glu Leu Gly Lys Tyr Glu Gln Tyr Ile Lys Trp Pro Trp Tyr Ile Trp           
        515                 520                 525                       

cta ggt ttt ata gct ggc ttg att gcc ata gta atg gtg                   1623
Leu Gly Phe Ile Ala Gly Leu Ile Ala Ile Val Met Val                       
    530                 535                 540                           


<210>  2
<211>  541
<212>  PRT
<213>  SARS-CoV-2 </213>

<400>  2

Gln Ser Ile Ile Ala Tyr Thr Met Ser Leu Arg Ala Glu Asn Ser Val 
1               5                   10                  15      


Ala Tyr Ser Asn Asn Ser Ile Ala Ile Pro Thr Asn Phe Thr Ile Ser 
            20                  25                  30          


Val Thr Thr Glu Ile Leu Pro Val Ser Met Thr Lys Thr Ser Val Asp 
        35                  40                  45              


Cys Thr Met Tyr Ile Cys Gly Asp Ser Thr Glu Cys Ser Asn Leu Leu 
    50                  55                  60                  


Leu Gln Tyr Gly Ser Phe Cys Thr Gln Leu Asn Arg Ala Leu Thr Gly 
65                  70                  75                  80  


Ile Ala Val Glu Gln Asp Lys Asn Thr Gln Glu Val Phe Ala Gln Val 
                85                  90                  95      


Lys Gln Ile Tyr Lys Thr Pro Pro Ile Lys Asp Phe Gly Gly Phe Asn 
            100                 105                 110         


Phe Ser Gln Ile Leu Pro Asp Pro Ser Lys Pro Ser Lys Arg Ser Phe 
        115                 120                 125             


Ile Glu Asp Leu Leu Phe Asn Lys Val Thr Leu Ala Asp Ala Gly Phe 
    130                 135                 140                 


Ile Lys Gln Tyr Gly Asp Cys Leu Gly Asp Ile Ala Ala Arg Asp Leu 
145                 150                 155                 160 


Ile Cys Ala Gln Lys Phe Asn Gly Leu Thr Val Leu Pro Pro Leu Leu 
                165                 170                 175     


Thr Asp Glu Met Ile Ala Gln Tyr Thr Ser Ala Leu Leu Ala Gly Thr 
            180                 185                 190         


Ile Thr Ser Gly Trp Thr Phe Gly Ala Gly Ala Ala Leu Gln Ile Pro 
        195                 200                 205             


Phe Ala Met Gln Met Ala Tyr Arg Phe Asn Gly Ile Gly Val Thr Gln 
    210                 215                 220                 


Asn Val Leu Tyr Glu Asn Gln Lys Leu Ile Ala Asn Gln Phe Asn Ser 
225                 230                 235                 240 


Ala Ile Gly Lys Ile Gln Asp Ser Leu Ser Ser Thr Ala Ser Ala Leu 
                245                 250                 255     


Gly Lys Leu Gln Asp Val Val Asn Gln Asn Ala Gln Ala Leu Asn Thr 
            260                 265                 270         


Leu Val Lys Gln Leu Ser Ser Asn Phe Gly Ala Ile Ser Ser Val Leu 
        275                 280                 285             


Asn Asp Ile Leu Ser Arg Leu Asp Lys Val Glu Ala Glu Val Gln Ile 
    290                 295                 300                 


Asp Arg Leu Ile Thr Gly Arg Leu Gln Ser Leu Gln Thr Tyr Val Thr 
305                 310                 315                 320 


Gln Gln Leu Ile Arg Ala Ala Glu Ile Arg Ala Ser Ala Asn Leu Ala 
                325                 330                 335     


Ala Thr Lys Met Ser Glu Cys Val Leu Gly Gln Ser Lys Arg Val Asp 
            340                 345                 350         


Phe Leu Gly Lys Gly Tyr His Leu Met Ser Phe Pro Gln Ser Ala Pro 
        355                 360                 365             


His Gly Val Val Phe Leu His Val Thr Tyr Val Pro Ala Gln Glu Lys 
    370                 375                 380                 


Asn Phe Thr Thr Ala Pro Ala Ile Cys His Asp Gly Lys Ala His Phe 
385                 390                 395                 400 


Pro Arg Glu Gly Val Phe Val Ser Asn Gly Thr His Trp Phe Val Thr 
                405                 410                 415     


Gln Arg Asn Phe Tyr Glu Pro Gln Ile Ile Thr Thr Asp Asn Thr Phe 
            420                 425                 430         


Val Ser Gly Asn Cys Asp Val Val Ile Gly Ile Val Asn Asn Thr Val 
        435                 440                 445             


Tyr Asp Pro Leu Gln Pro Glu Leu Asp Ser Phe Lys Glu Glu Leu Asp 
    450                 455                 460                 


Lys Tyr Phe Lys Asn His Thr Ser Pro Asp Val Asp Leu Gly Asp Ile 
465                 470                 475                 480 


Ser Gly Ile Asn Ala Ser Val Val Asn Ile Gln Lys Glu Ile Asp Arg 
                485                 490                 495     


Leu Asn Glu Val Ala Lys Asn Leu Asn Glu Ser Leu Ile Asp Leu Gln 
            500                 505                 510         


Glu Leu Gly Lys Tyr Glu Gln Tyr Ile Lys Trp Pro Trp Tyr Ile Trp 
        515                 520                 525             


Leu Gly Phe Ile Ala Gly Leu Ile Ala Ile Val Met Val 
    530                 535                 540     


<210>  3
<211>  648
<212>  DNA
<213>  SARS-CoV-2 </213>


<220>
<221>  CDS
<222>  (1)..(648)
<223>  Domaine S1 de la protine S

<400>  3
act agg ttt caa act tta ctt gct tta cat aga agt tat ttg act cct         48
Thr Arg Phe Gln Thr Leu Leu Ala Leu His Arg Ser Tyr Leu Thr Pro           
1               5                   10                  15                

ggt gat tct tct tca ggt tgg aca gct ggt gct gca gct tat tat gtg         96
Gly Asp Ser Ser Ser Gly Trp Thr Ala Gly Ala Ala Ala Tyr Tyr Val           
            20                  25                  30                    

ggt tat ctt caa cct agg act ttt cta tta aaa tat aat gaa aat gga        144
Gly Tyr Leu Gln Pro Arg Thr Phe Leu Leu Lys Tyr Asn Glu Asn Gly           
        35                  40                  45                        

acc att aca gat gct gta gac tgt gca ctt gac cct ctc tca gaa aca        192
Thr Ile Thr Asp Ala Val Asp Cys Ala Leu Asp Pro Leu Ser Glu Thr           
    50                  55                  60                            

aag tgt acg ttg aaa tcc ttc act gta gaa aaa gga atc tat caa act        240
Lys Cys Thr Leu Lys Ser Phe Thr Val Glu Lys Gly Ile Tyr Gln Thr           
65                  70                  75                  80            

tct aac ttt aga gtc caa cca aca gaa tct att gtt aga ttt cct aat        288
Ser Asn Phe Arg Val Gln Pro Thr Glu Ser Ile Val Arg Phe Pro Asn           
                85                  90                  95                

att aca aac ttg tgc cct ttt ggt gaa gtt ttt aac gcc acc aga ttt        336
Ile Thr Asn Leu Cys Pro Phe Gly Glu Val Phe Asn Ala Thr Arg Phe           
            100                 105                 110                   

gca tct gtt tat gct tgg aac agg aag aga atc agc aac tgt gtt gct        384
Ala Ser Val Tyr Ala Trp Asn Arg Lys Arg Ile Ser Asn Cys Val Ala           
        115                 120                 125                       

gat tat tct gtc cta tat aat tcc gca tca ttt tcc act ttt aag tgt        432
Asp Tyr Ser Val Leu Tyr Asn Ser Ala Ser Phe Ser Thr Phe Lys Cys           
    130                 135                 140                           

tat gga gtg tct cct act aaa tta aat gat ctc tgc ttt act aat gtc        480
Tyr Gly Val Ser Pro Thr Lys Leu Asn Asp Leu Cys Phe Thr Asn Val           
145                 150                 155                 160           

tat gca gat tca ttt gta att aga ggt gat gaa gtc aga caa atc gct        528
Tyr Ala Asp Ser Phe Val Ile Arg Gly Asp Glu Val Arg Gln Ile Ala           
                165                 170                 175               

cca ggg caa act gga aag att gct gat tat aat tat aaa tta cca gat        576
Pro Gly Gln Thr Gly Lys Ile Ala Asp Tyr Asn Tyr Lys Leu Pro Asp           
            180                 185                 190                   

gat ttt aca ggc tgc gtt ata gct tgg aat tct aac aat ctt gat tct        624
Asp Phe Thr Gly Cys Val Ile Ala Trp Asn Ser Asn Asn Leu Asp Ser           
        195                 200                 205                       

aag gtt ggt ggt aat tat aat tac                                        648
Lys Val Gly Gly Asn Tyr Asn Tyr                                           
    210                 215                                               


<210>  4
<211>  216
<212>  PRT
<213>  SARS-CoV-2 </213>

<400>  4

Thr Arg Phe Gln Thr Leu Leu Ala Leu His Arg Ser Tyr Leu Thr Pro 
1               5                   10                  15      


Gly Asp Ser Ser Ser Gly Trp Thr Ala Gly Ala Ala Ala Tyr Tyr Val 
            20                  25                  30          


Gly Tyr Leu Gln Pro Arg Thr Phe Leu Leu Lys Tyr Asn Glu Asn Gly 
        35                  40                  45              


Thr Ile Thr Asp Ala Val Asp Cys Ala Leu Asp Pro Leu Ser Glu Thr 
    50                  55                  60                  


Lys Cys Thr Leu Lys Ser Phe Thr Val Glu Lys Gly Ile Tyr Gln Thr 
65                  70                  75                  80  


Ser Asn Phe Arg Val Gln Pro Thr Glu Ser Ile Val Arg Phe Pro Asn 
                85                  90                  95      


Ile Thr Asn Leu Cys Pro Phe Gly Glu Val Phe Asn Ala Thr Arg Phe 
            100                 105                 110         


Ala Ser Val Tyr Ala Trp Asn Arg Lys Arg Ile Ser Asn Cys Val Ala 
        115                 120                 125             


Asp Tyr Ser Val Leu Tyr Asn Ser Ala Ser Phe Ser Thr Phe Lys Cys 
    130                 135                 140                 


Tyr Gly Val Ser Pro Thr Lys Leu Asn Asp Leu Cys Phe Thr Asn Val 
145                 150                 155                 160 


Tyr Ala Asp Ser Phe Val Ile Arg Gly Asp Glu Val Arg Gln Ile Ala 
                165                 170                 175     


Pro Gly Gln Thr Gly Lys Ile Ala Asp Tyr Asn Tyr Lys Leu Pro Asp 
            180                 185                 190         


Asp Phe Thr Gly Cys Val Ile Ala Trp Asn Ser Asn Asn Leu Asp Ser 
        195                 200                 205             


Lys Val Gly Gly Asn Tyr Asn Tyr 
    210                 215     


<210>  5
<211>  432
<212>  DNA
<213>  SARS-CoV-2 </213>


<220>
<221>  CDS
<222>  (1)..(432)
<223>  Protine N

<400>  5
gct gct ctt gct ttg ctg ctg ctt gac aga ttg aac cag ctt gag agc         48
Ala Ala Leu Ala Leu Leu Leu Leu Asp Arg Leu Asn Gln Leu Glu Ser           
1               5                   10                  15                

aaa atg tct ggt aaa ggc caa caa caa caa ggc caa act gtc act aag         96
Lys Met Ser Gly Lys Gly Gln Gln Gln Gln Gly Gln Thr Val Thr Lys           
            20                  25                  30                    

aaa tct gct gct gag gct tct aag aag cct cgg caa aaa cgt act gcc        144
Lys Ser Ala Ala Glu Ala Ser Lys Lys Pro Arg Gln Lys Arg Thr Ala           
        35                  40                  45                        

act aaa gca tac aat gta aca caa gct ttc ggc aga cgt ggt cca gaa        192
Thr Lys Ala Tyr Asn Val Thr Gln Ala Phe Gly Arg Arg Gly Pro Glu           
    50                  55                  60                            

caa acc caa gga aat ttt ggg gac cag gaa cta atc aga caa gga act        240
Gln Thr Gln Gly Asn Phe Gly Asp Gln Glu Leu Ile Arg Gln Gly Thr           
65                  70                  75                  80            

gat tac aaa cat tgg ccg caa att gca caa ttt gcc ccc agc gct tca        288
Asp Tyr Lys His Trp Pro Gln Ile Ala Gln Phe Ala Pro Ser Ala Ser           
                85                  90                  95                

gcg ttc ttc gga atg tcg cgc att ggc atg gaa gtc aca cct tcg gga        336
Ala Phe Phe Gly Met Ser Arg Ile Gly Met Glu Val Thr Pro Ser Gly           
            100                 105                 110                   

acg tgg ttg acc tac aca ggt gcc atc aaa ttg gat gac aaa gat cca        384
Thr Trp Leu Thr Tyr Thr Gly Ala Ile Lys Leu Asp Asp Lys Asp Pro           
        115                 120                 125                       

aat ttc aaa gat caa gtc att ttg ctg aat aag cat att gac gca tac        432
Asn Phe Lys Asp Gln Val Ile Leu Leu Asn Lys His Ile Asp Ala Tyr           
    130                 135                 140                           


<210>  6
<211>  144
<212>  PRT
<213>  SARS-CoV-2 </213>

<400>  6

Ala Ala Leu Ala Leu Leu Leu Leu Asp Arg Leu Asn Gln Leu Glu Ser 
1               5                   10                  15      


Lys Met Ser Gly Lys Gly Gln Gln Gln Gln Gly Gln Thr Val Thr Lys 
            20                  25                  30          


Lys Ser Ala Ala Glu Ala Ser Lys Lys Pro Arg Gln Lys Arg Thr Ala 
        35                  40                  45              


Thr Lys Ala Tyr Asn Val Thr Gln Ala Phe Gly Arg Arg Gly Pro Glu 
    50                  55                  60                  


Gln Thr Gln Gly Asn Phe Gly Asp Gln Glu Leu Ile Arg Gln Gly Thr 
65                  70                  75                  80  


Asp Tyr Lys His Trp Pro Gln Ile Ala Gln Phe Ala Pro Ser Ala Ser 
                85                  90                  95      


Ala Phe Phe Gly Met Ser Arg Ile Gly Met Glu Val Thr Pro Ser Gly 
            100                 105                 110         


Thr Trp Leu Thr Tyr Thr Gly Ala Ile Lys Leu Asp Asp Lys Asp Pro 
        115                 120                 125             


Asn Phe Lys Asp Gln Val Ile Leu Leu Asn Lys His Ile Asp Ala Tyr 
    130                 135                 140                 


