                         SEQUENCE LISTING

<110>  Oxford Biomedica (UK) Limited
 
<120>  Viral vector production

<130>  P297050WO

<150>  GB 2007199.9
<151>  2020-05-15

<160>  74    

<170>  PatentIn version 3.5

<210>  1
<211>  7
<212>  RNA
<213>  Homo sapiens


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  M is A or C

<220>
<221>  misc_feature
<222>  (6)..(7)
<223>  R is A or G

<400>  1
maggurr                                                                  7


<210>  2
<211>  9
<212>  RNA
<213>  Homo sapiens

<400>  2
acuuaccug                                                                9


<210>  3
<211>  8
<212>  RNA
<213>  Homo sapiens

<400>  3
auuugugg                                                                 8


<210>  4
<211>  153
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  main U1 snRNA sequence (nt 410-562) of the U1_256 sequence

<400>  4
gcaggggaga taccatgatc acgaaggtgg ttttcccagg gcgaggctta tccattgcac       60

tccggatgtg ctgacccctg cgatttcccc aaatgtggga aactcgactg cataatttgt      120

ggtagtgggg gactgcgttc gcgctttccc ctg                                   153


<210>  5
<211>  7
<212>  DNA
<213>  Unknown

<220>
<223>  branch point consensus sequence in eukaryotes


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  Y is a pyrimidine

<220>
<221>  misc_feature
<222>  (2)..(2)
<223>  N is any nucleotide

<220>
<221>  misc_feature
<222>  (3)..(3)
<223>  Y is a pyrimidine

<220>
<221>  misc_feature
<222>  (5)..(5)
<223>  R is a purine

<400>  5
ynytrac                                                                  7


<210>  6
<211>  3
<212>  RNA
<213>  Unknown

<220>
<223>  3' acceptor splice site consensus sequence in eukaryotes


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  Y is a pyrimidine

<400>  6
yag                                                                      3


<210>  7
<211>  8
<212>  DNA
<213>  Unknown

<220>
<223>  canonical splice donor site consensus sequence


<220>
<221>  misc_feature
<222>  (2)..(3)
<223>  cleavage site

<220>
<221>  misc_feature
<222>  (5)..(5)
<223>  R is a purine

<400>  7
aggtragt                                                                 8


<210>  8
<211>  8
<212>  DNA
<213>  Human immunodeficiency virus type 1


<220>
<221>  misc_feature
<222>  (2)..(3)
<223>  cleavage site

<220>
<221>  misc_feature
<222>  (5)..(5)
<223>  R is a purine

<400>  8
tggtragt                                                                 8


<210>  9
<211>  30
<212>  DNA
<213>  Unknown

<220>
<223>  splice donor region of the lentiviral vector genome

<400>  9
ggggcggcga ctggtgagta cgccaaaaat                                        30


<210>  10
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  MSD-2KO mutated splice donor region

<400>  10
ggggcggcga ctgcagacaa cgccaaaaat                                        30


<210>  11
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  MSD-2KOv2 mutated splice donor region

<400>  11
ggggcggcga gtggagacta cgccaaaaat                                        30


<210>  12
<211>  32
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  MSD-2KOm5 mutated splice donor region

<400>  12
ggggaaggca acagataaat atgccttaaa at                                     32


<210>  13
<211>  19
<212>  DNA
<213>  Unknown

<220>
<223>  splice donor region of lentiviral genome

<400>  13
ggcgactggt gagtacgcc                                                    19


<210>  14
<211>  7
<212>  DNA
<213>  Unknown

<220>
<223>  major splice donor and cryptic splice donor region core sequence


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  cleavage site

<220>
<221>  misc_feature
<222>  (4)..(5)
<223>  cleavage site

<400>  14
gtgagta                                                                  7


<210>  15
<211>  5
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  major splice donor consensus sequence

<400>  15
ctggt                                                                    5


<210>  16
<211>  5
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  cryptic splice donor site consensus sequence

<400>  16
tgagt                                                                    5


<210>  17
<211>  6
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  splice donor region

<400>  17
cagaca                                                                   6


<210>  18
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  mutated splice donor region

<400>  18
ggcgactgca gacaacgcc                                                    19


<210>  19
<211>  9
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  mutated splice donor region

<400>  19
gtggagact                                                                9


<210>  20
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  mutated splice donor region

<400>  20
ggcgagtgga gactacgcc                                                    19


<210>  21
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  mutated splice donor region

<400>  21
aaggcaacag ataaatatgc ctt                                               23


<210>  22
<211>  642
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DNA-based expression constructs for the modified U1 snRNAs

<400>  22
taaggaccag cttctttggg agagaacaga cgcaggggcg ggagggaaaa agggagaggc       60

agacgtcact tccccttggc ggctctggca gcagattggt cggttgagtg gcagaaaggc      120

agacggggac tgggcaaggc actgtcggtg acatcacgga cagggcgact tctatgtaga      180

tgaggcagcg cagaggctgc tgcttcgcca cttgctgctt caccacgaag gagttcccgt      240

gccctgggag cgggttcagg accgctgatc ggaagtgaga atcccagctg tgtgtcaggg      300

ctggaaaggg ctcgggagtg cgcggggcaa gtgaccgtgt gtgtaaagag tgaggcgtat      360

gaggctgtgt cggggcagag gcccaagatc tcatttgccg tgcgcgcttg caggggagat      420

accatgatca cgaaggtggt tttcccaggg cgaggcttat ccattgcact ccggatgtgc      480

tgacccctgc gatttcccca aatgtgggaa actcgactgc ataatttgtg gtagtggggg      540

actgcgttcg cgctttcccc tggtttcaaa agtagactgt acgctaaggg tcatatcttt      600

ttttgttttg gtttgtgtct tggttggcgt cttaaatgtt aa                         642


<210>  23
<211>  15
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  U1_16 HIV-1 target sequence [NL4-3]

<400>  23
gaccagatct gagcc                                                        15


<210>  24
<211>  17
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  U1_16 U1 snRNA target-annealing sequence

<400>  24
atggctcaga tctggtc                                                      17


<210>  25
<211>  15
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  U1_31 HIV-1 target sequence [NL4-3]

<400>  25
tgggagctct ctggc                                                        15


<210>  26
<211>  17
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  U1_31 U1 snRNA target-annealing sequence

<400>  26
atgccagaga gctccca                                                      17


<210>  27
<211>  15
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  U1_76 HIV-1 target sequence [NL4-3]

<400>  27
taaagcttgc cttga                                                        15


<210>  28
<211>  17
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  U1_76 U1 snRNA target-annealing sequence

<400>  28
attcaaggca agcttta                                                      17


<210>  29
<211>  15
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  U1_136 HIV-1 target sequence [NL4-3]

<400>  29
tagagatccc tcaga                                                        15


<210>  30
<211>  17
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  U1_136 U1 snRNA target-annealing sequence

<400>  30
attctgaggg atctcta                                                      17


<210>  31
<211>  9
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  U1_179(9nt) HIV-1 target sequence [NL4-3]

<400>  31
gcagtggcg                                                                9


<210>  32
<211>  11
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  U1_179(9nt) U1 snRNA target-annealing sequence

<400>  32
atcgccactg c                                                            11


<210>  33
<211>  15
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  U1_181 HIV-1 target sequence [NL4-3]

<400>  33
agtggcgccc gaaca                                                        15


<210>  34
<211>  17
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  U1_181 U1 snRNA target-annealing sequence

<400>  34
attgttcggg cgccact                                                      17


<210>  35
<211>  15
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  U1_196 HIV-1 target sequence [NL4-3]

<400>  35
gggacttgaa agcga                                                        15


<210>  36
<211>  17
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  U1_196 U1 snRNA target-annealing sequence

<400>  36
attcgctttc aagtccc                                                      17


<210>  37
<211>  15
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  U1_211 HIV-1 target sequence [NL4-3]

<400>  37
aagggaaacc agagg                                                        15


<210>  38
<211>  17
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  U1_211 U1 snRNA target-annealing sequence

<400>  38
atcctctggt ttccctt                                                      17


<210>  39
<211>  15
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  U1_226 HIV-1 target sequence [NL4-3]

<400>  39
agctctctcg acgca                                                        15


<210>  40
<211>  17
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  U1_226 U1 snRNA target-annealing sequence

<400>  40
attgcgtcga gagagct                                                      17


<210>  41
<211>  15
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  U1_241 HIV-1 target sequence [NL4-3]

<400>  41
ggactcggct tgctg                                                        15


<210>  42
<211>  17
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  U1_241 U1 snRNA target-annealing sequence

<400>  42
atcagcaagc cgagtcc                                                      17


<210>  43
<211>  15
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  U1_256 HIV-1 target sequence [NL4-3]

<400>  43
aagcgcgcac ggcaa                                                        15


<210>  44
<211>  17
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  U1_256 U1 snRNA target-annealing sequence

<400>  44
atttgccgtg cgcgctt                                                      17


<210>  45
<211>  15
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  U1_271 HIV-1 target sequence [NL4-3]

<400>  45
gaggcgaggg gcggc                                                        15


<210>  46
<211>  17
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  U1_271 U1 snRNA target-annealing sequence

<400>  46
atgccgcccc tcgcctc                                                      17


<210>  47
<211>  15
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  U1_286 HIV-1 target sequence [NL4-3]

<400>  47
gactggtgag tacgc                                                        15


<210>  48
<211>  17
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  U1_286 U1 snRNA target-annealing sequence

<400>  48
atgcgtactc accagtc                                                      17


<210>  49
<211>  11
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  U1_305(9nt) HIV-1 target sequence [NL4-3]

<400>  49
aattttgact a                                                            11


<210>  50
<211>  11
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  U1_305(9nt) U1 snRNA target-annealing sequence

<400>  50
atgtcaaaat t                                                            11


<210>  51
<211>  15
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  U1_305 HIV-1 target sequence [NL4-3]

<400>  51
aattttgact agcgg                                                        15


<210>  52
<211>  17
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  U1_305 U1 snRNA target-annealing sequence

<400>  52
atccgctagt caaaatt                                                      17


<210>  53
<211>  15
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  U1_316 HIV-1 target sequence [NL4-3]

<400>  53
gcggaggcta gaagg                                                        15


<210>  54
<211>  17
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  U1_316 U1 snRNA target-annealing sequence

<400>  54
atccttctag cctccgc                                                      17


<210>  55
<211>  15
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  U1_331 HIV-1 target sequence [NL4-3]

<400>  55
agagagatgg gtgcg                                                        15


<210>  56
<211>  17
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  U1_331 U1 snRNA target-annealing sequence

<400>  56
atcgcaccca tctctct                                                      17


<210>  57
<211>  15
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  U1_346 HIV-1 target sequence [NL4-3]

<400>  57
agagcgtcgg tatta                                                        15


<210>  58
<211>  17
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  U1_346 U1 snRNA target-annealing sequence

<400>  58
attaatactg acgctct                                                      17


<210>  59
<211>  15
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  U1_361 HIV-1 target sequence [NL4-3]

<400>  59
agcgggggag aatta                                                        15


<210>  60
<211>  17
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  U1_361 U1 snRNA target-annealing sequence

<400>  60
attaattctc ccccgct                                                      17


<210>  61
<211>  15
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  U1_376 HIV-1 target sequence [NL4-3]

<400>  61
gatcgcgatg ggaaa                                                        15


<210>  62
<211>  17
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  U1_376 U1 snRNA target-annealing sequence

<400>  62
attttcccat cgcgatc                                                      17


<210>  63
<211>  15
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  U1_391 HIV-1 target sequence [NL4-3]

<400>  63
aaattcggtt aaggc                                                        15


<210>  64
<211>  17
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  U1_391 U1 snRNA target-annealing sequence

<400>  64
atgccttaac cgaattt                                                      17


<210>  65
<211>  15
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  U1_690 HIV-1 target sequence [NL4-3]

<400>  65
gatcttcaga cctgg                                                        15


<210>  66
<211>  17
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  U1_690 U1 snRNA target-annealing sequence

<400>  66
atccaggtct gaagatc                                                      17


<210>  67
<211>  15
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  U1_1203 HIV-1 target sequence [NL4-3]

<400>  67
ttacacaagc ttaat                                                        15


<210>  68
<211>  17
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  U1_1203 U1 snRNA target-annealing sequence

<400>  68
atattaagct tgtgtaa                                                      17


<210>  69
<211>  15
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  U1_1546 HIV-1 target sequence [NL4-3]

<400>  69
tagtagacat aatag                                                        15


<210>  70
<211>  17
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  U1_1546 U1 snRNA target-annealing sequence

<400>  70
atctattatg tctacta                                                      17


<210>  71
<211>  9
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  U1_LacZ1 Target sequence

<400>  71
ctacaggaa                                                                9


<210>  72
<211>  11
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  U1_LacZ1 U1 snRNA target-annealing sequence

<400>  72
atttcctgta g                                                            11


<210>  73
<211>  9
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  U1_LacZ2 Target sequence

<400>  73
tcatctgtg                                                                9


<210>  74
<211>  11
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  U1_LacZ2 U1 snRNA target-annealing sequence

<400>  74
atcacagatg a                                                            11


