                         SEQUENCE LISTING

<110>  Autolus Limited
 
<120>  METHOD

<130>  P120285PCT

<150>  GB 2007044.7
<151>  2020-05-13

<160>  104   

<170>  PatentIn version 3.5

<210>  1
<211>  540
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  flexi-IL-12 sequence

<400>  1

Met Glu Thr Asp Thr Leu Leu Leu Trp Val Leu Leu Leu Trp Val Pro 
1               5                   10                  15      


Gly Ser Thr Gly Met Trp Ile Trp Glu Leu Lys Lys Asp Val Tyr Val 
            20                  25                  30          


Val Glu Leu Asp Trp Tyr Pro Asp Ala Pro Gly Glu Met Val Val Leu 
        35                  40                  45              


Thr Cys Asp Thr Pro Glu Glu Asp Gly Ile Thr Trp Thr Leu Asp Gln 
    50                  55                  60                  


Ser Ser Glu Val Leu Gly Ser Gly Lys Thr Leu Thr Ile Gln Val Lys 
65                  70                  75                  80  


Glu Phe Gly Asp Ala Gly Gln Tyr Thr Cys His Lys Gly Gly Glu Val 
                85                  90                  95      


Leu Ser His Ser Leu Leu Leu Leu His Lys Lys Glu Asp Gly Ile Trp 
            100                 105                 110         


Ser Thr Asp Ile Leu Lys Asp Gln Lys Glu Pro Lys Asn Lys Thr Phe 
        115                 120                 125             


Leu Arg Cys Glu Ala Lys Asn Tyr Ser Gly Arg Phe Thr Cys Trp Trp 
    130                 135                 140                 


Leu Thr Thr Ile Ser Thr Asp Leu Thr Phe Ser Val Lys Ser Ser Arg 
145                 150                 155                 160 


Gly Ser Ser Asp Pro Gln Gly Val Thr Cys Gly Ala Ala Thr Leu Ser 
                165                 170                 175     


Ala Glu Arg Val Arg Gly Asp Asn Lys Glu Tyr Glu Tyr Ser Val Glu 
            180                 185                 190         


Cys Gln Glu Asp Ser Ala Cys Pro Ala Ala Glu Glu Ser Leu Pro Ile 
        195                 200                 205             


Glu Val Met Val Asp Ala Val His Lys Leu Lys Tyr Glu Asn Tyr Thr 
    210                 215                 220                 


Ser Ser Phe Phe Ile Arg Asp Ile Ile Lys Pro Asp Pro Pro Lys Asn 
225                 230                 235                 240 


Leu Gln Leu Lys Pro Leu Lys Asn Ser Arg Gln Val Glu Val Ser Trp 
                245                 250                 255     


Glu Tyr Pro Asp Thr Trp Ser Thr Pro His Ser Tyr Phe Ser Leu Thr 
            260                 265                 270         


Phe Cys Val Gln Val Gln Gly Lys Ser Lys Arg Glu Lys Lys Asp Arg 
        275                 280                 285             


Val Phe Thr Asp Lys Thr Ser Ala Thr Val Ile Cys Arg Lys Asn Ala 
    290                 295                 300                 


Ser Ile Ser Val Arg Ala Gln Asp Arg Tyr Tyr Ser Ser Ser Trp Ser 
305                 310                 315                 320 


Glu Trp Ala Ser Val Pro Cys Ser Gly Gly Gly Gly Ser Gly Gly Gly 
                325                 330                 335     


Gly Ser Gly Gly Gly Gly Ser Arg Asn Leu Pro Leu Ala Thr Pro Asp 
            340                 345                 350         


Pro Gly Met Phe Pro Cys Leu His His Ser Gln Asn Leu Leu Arg Ala 
        355                 360                 365             


Val Ser Asn Met Leu Gln Lys Ala Arg Gln Thr Leu Glu Phe Tyr Pro 
    370                 375                 380                 


Cys Thr Ser Glu Glu Ile Asp His Glu Asp Ile Thr Lys Asp Lys Thr 
385                 390                 395                 400 


Ser Thr Val Glu Ala Cys Leu Pro Leu Glu Leu Thr Lys Asn Glu Ser 
                405                 410                 415     


Cys Leu Asn Ser Arg Glu Thr Ser Phe Ile Thr Asn Gly Ser Cys Leu 
            420                 425                 430         


Ala Ser Arg Lys Thr Ser Phe Met Met Ala Leu Cys Leu Ser Ser Ile 
        435                 440                 445             


Tyr Glu Asp Ser Lys Met Tyr Gln Val Glu Phe Lys Thr Met Asn Ala 
    450                 455                 460                 


Lys Leu Leu Met Asp Pro Lys Arg Gln Ile Phe Leu Asp Gln Asn Met 
465                 470                 475                 480 


Leu Ala Val Ile Asp Glu Leu Met Gln Ala Leu Asn Phe Asn Ser Glu 
                485                 490                 495     


Thr Val Pro Gln Lys Ser Ser Leu Glu Glu Pro Asp Phe Tyr Lys Thr 
            500                 505                 510         


Lys Ile Lys Leu Cys Ile Leu Leu His Ala Phe Arg Ile Arg Ala Val 
        515                 520                 525             


Thr Ile Asp Arg Val Met Ser Tyr Leu Asn Ala Ser 
    530                 535                 540 


<210>  2
<211>  7
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  frame-slip motif (FSM) sequence

<400>  2
uuuuuuu                                                                  7


<210>  3
<211>  9
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  FSM sequence

<400>  3
uuuuuuuga                                                                9


<210>  4
<211>  9
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  FSM sequence

<400>  4
uuuuuuuag                                                                9


<210>  5
<211>  9
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  FSM sequence

<400>  5
uuuuuuuaa                                                                9


<210>  6
<211>  7
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  translational readthrough motif (TRM) sequence

<400>  6
ugacuag                                                                  7


<210>  7
<211>  7
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  TRM sequence

<400>  7
uagcuag                                                                  7


<210>  8
<211>  7
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  TRM sequence

<400>  8
uaacuag                                                                  7


<210>  9
<211>  9
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  TRM sequence

<400>  9
ugacaauua                                                                9


<210>  10
<211>  9
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  TRM sequence

<400>  10
uagcaauua                                                                9


<210>  11
<211>  9
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  TRM sequence

<400>  11
uaacaauua                                                                9


<210>  12
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  heavy chain variable region (VH) complementarity determining 
       region (CDR), CDR1

<400>  12

Ser Tyr Asn Ile His 
1               5   


<210>  13
<211>  16
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VH CDR, CDR2

<400>  13

Val Ile Trp Ala Gly Gly Ser Thr Asn Tyr Asn Ser Ala Leu Met Ser 
1               5                   10                  15      


<210>  14
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VH CDR, CDR3

<400>  14

Arg Ser Asp Asp Tyr Ser Trp Phe Ala Tyr 
1               5                   10  


<210>  15
<211>  12
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  light chain variable region (VL) CDR, CDR1

<400>  15

Arg Ala Ser Ser Ser Val Ser Ser Ser Tyr Leu His 
1               5                   10          


<210>  16
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VL CDR, CDR2

<400>  16

Ser Thr Ser Asn Leu Ala Ser 
1               5           


<210>  17
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VL CDR, CDR3

<400>  17

Gln Gln Tyr Ser Gly Tyr Pro Ile Thr 
1               5                   


<210>  18
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VH CDR, CDR1

<400>  18

Ser Ser Trp Met Asn 
1               5   


<210>  19
<211>  17
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VH CDR, CDR2

<400>  19

Arg Ile Tyr Pro Gly Asp Gly Asp Thr Asn Tyr Ala Gln Lys Phe Gln 
1               5                   10                  15      


Gly 
    


<210>  20
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VH CDR, CDR3

<400>  20

Gly Thr Gly Tyr Leu Trp Tyr Phe Asp Val 
1               5                   10  


<210>  21
<211>  11
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VL CDR, CDR1

<400>  21

Arg Ala Ser Gln Asp Ile Asn Glu Asn Leu Ala 
1               5                   10      


<210>  22
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VL CDR, CDR2

<400>  22

Tyr Thr Ser Asn Arg Ala Thr 
1               5           


<210>  23
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VL CDR, CDR3

<400>  23

Gln Gln Tyr Asp Asn Leu Pro Phe Thr 
1               5                   


<210>  24
<211>  197
<212>  PRT
<213>  Homo sapiens

<400>  24

Arg Asn Leu Pro Val Ala Thr Pro Asp Pro Gly Met Phe Pro Cys Leu 
1               5                   10                  15      


His His Ser Gln Asn Leu Leu Arg Ala Val Ser Asn Met Leu Gln Lys 
            20                  25                  30          


Ala Arg Gln Thr Leu Glu Phe Tyr Pro Cys Thr Ser Glu Glu Ile Asp 
        35                  40                  45              


His Glu Asp Ile Thr Lys Asp Lys Thr Ser Thr Val Glu Ala Cys Leu 
    50                  55                  60                  


Pro Leu Glu Leu Thr Lys Asn Glu Ser Cys Leu Asn Ser Arg Glu Thr 
65                  70                  75                  80  


Ser Phe Ile Thr Asn Gly Ser Cys Leu Ala Ser Arg Lys Thr Ser Phe 
                85                  90                  95      


Met Met Ala Leu Cys Leu Ser Ser Ile Tyr Glu Asp Leu Lys Met Tyr 
            100                 105                 110         


Gln Val Glu Phe Lys Thr Met Asn Ala Lys Leu Leu Met Asp Pro Lys 
        115                 120                 125             


Arg Gln Ile Phe Leu Asp Gln Asn Met Leu Ala Val Ile Asp Glu Leu 
    130                 135                 140                 


Met Gln Ala Leu Asn Phe Asn Ser Glu Thr Val Pro Gln Lys Ser Ser 
145                 150                 155                 160 


Leu Glu Glu Pro Asp Phe Tyr Lys Thr Lys Ile Lys Leu Cys Ile Leu 
                165                 170                 175     


Leu His Ala Phe Arg Ile Arg Ala Val Thr Ile Asp Arg Val Met Ser 
            180                 185                 190         


Tyr Leu Asn Ala Ser 
        195         


<210>  25
<211>  304
<212>  PRT
<213>  Homo sapiens

<400>  25

Trp Glu Leu Lys Lys Asp Val Tyr Val Val Glu Leu Asp Trp Tyr Pro 
1               5                   10                  15      


Asp Ala Pro Gly Glu Met Val Val Leu Thr Cys Asp Thr Pro Glu Glu 
            20                  25                  30          


Asp Gly Ile Thr Trp Thr Leu Asp Gln Ser Ser Glu Val Leu Gly Ser 
        35                  40                  45              


Gly Lys Thr Leu Thr Ile Gln Val Lys Glu Phe Gly Asp Ala Gly Gln 
    50                  55                  60                  


Tyr Thr Cys His Lys Gly Gly Glu Val Leu Ser His Ser Leu Leu Leu 
65                  70                  75                  80  


Leu His Lys Lys Glu Asp Gly Ile Trp Ser Thr Asp Ile Leu Lys Asp 
                85                  90                  95      


Gln Lys Glu Pro Lys Asn Lys Thr Phe Leu Arg Cys Glu Ala Lys Asn 
            100                 105                 110         


Tyr Ser Gly Arg Phe Thr Cys Trp Trp Leu Thr Thr Ile Ser Thr Asp 
        115                 120                 125             


Leu Thr Phe Ser Val Lys Ser Ser Arg Gly Ser Ser Asp Pro Gln Gly 
    130                 135                 140                 


Val Thr Cys Gly Ala Ala Thr Leu Ser Ala Glu Arg Val Arg Gly Asp 
145                 150                 155                 160 


Asn Lys Glu Tyr Glu Tyr Ser Val Glu Cys Gln Glu Asp Ser Ala Cys 
                165                 170                 175     


Pro Ala Ala Glu Glu Ser Leu Pro Ile Glu Val Met Val Asp Ala Val 
            180                 185                 190         


His Lys Leu Lys Tyr Glu Asn Tyr Thr Ser Ser Phe Phe Ile Arg Asp 
        195                 200                 205             


Ile Ile Lys Pro Asp Pro Pro Lys Asn Leu Gln Leu Lys Pro Leu Lys 
    210                 215                 220                 


Asn Ser Arg Gln Val Glu Val Ser Trp Glu Tyr Pro Asp Thr Trp Ser 
225                 230                 235                 240 


Thr Pro His Ser Tyr Phe Ser Leu Thr Phe Cys Val Gln Val Gln Gly 
                245                 250                 255     


Lys Ser Lys Arg Glu Lys Lys Asp Arg Val Phe Thr Asp Lys Thr Ser 
            260                 265                 270         


Ala Thr Val Ile Cys Arg Lys Asn Ala Ser Ile Ser Val Arg Ala Gln 
        275                 280                 285             


Asp Arg Tyr Tyr Ser Ser Ser Trp Ser Glu Trp Ala Ser Val Pro Cys 
    290                 295                 300                 


<210>  26
<211>  177
<212>  PRT
<213>  Homo sapiens

<400>  26

Met Phe His Val Ser Phe Arg Tyr Ile Phe Gly Leu Pro Pro Leu Ile 
1               5                   10                  15      


Leu Val Leu Leu Pro Val Ala Ser Ser Asp Cys Asp Ile Glu Gly Lys 
            20                  25                  30          


Asp Gly Lys Gln Tyr Glu Ser Val Leu Met Val Ser Ile Asp Gln Leu 
        35                  40                  45              


Leu Asp Ser Met Lys Glu Ile Gly Ser Asn Cys Leu Asn Asn Glu Phe 
    50                  55                  60                  


Asn Phe Phe Lys Arg His Ile Cys Asp Ala Asn Lys Glu Gly Met Phe 
65                  70                  75                  80  


Leu Phe Arg Ala Ala Arg Lys Leu Arg Gln Phe Leu Lys Met Asn Ser 
                85                  90                  95      


Thr Gly Asp Phe Asp Leu His Leu Leu Lys Val Ser Glu Gly Thr Thr 
            100                 105                 110         


Ile Leu Leu Asn Cys Thr Gly Gln Val Lys Gly Arg Lys Pro Ala Ala 
        115                 120                 125             


Leu Gly Glu Ala Gln Pro Thr Lys Ser Leu Glu Glu Asn Lys Ser Leu 
    130                 135                 140                 


Lys Glu Gln Lys Lys Leu Asn Asp Leu Cys Phe Leu Lys Arg Leu Leu 
145                 150                 155                 160 


Gln Glu Ile Lys Thr Cys Trp Asn Lys Ile Leu Met Gly Thr Lys Glu 
                165                 170                 175     


His 
    


<210>  27
<211>  162
<212>  PRT
<213>  Homo sapiens

<400>  27

Met Arg Ile Ser Lys Pro His Leu Arg Ser Ile Ser Ile Gln Cys Tyr 
1               5                   10                  15      


Leu Cys Leu Leu Leu Asn Ser His Phe Leu Thr Glu Ala Gly Ile His 
            20                  25                  30          


Val Phe Ile Leu Gly Cys Phe Ser Ala Gly Leu Pro Lys Thr Glu Ala 
        35                  40                  45              


Asn Trp Val Asn Val Ile Ser Asp Leu Lys Lys Ile Glu Asp Leu Ile 
    50                  55                  60                  


Gln Ser Met His Ile Asp Ala Thr Leu Tyr Thr Glu Ser Asp Val His 
65                  70                  75                  80  


Pro Ser Cys Lys Val Thr Ala Met Lys Cys Phe Leu Leu Glu Leu Gln 
                85                  90                  95      


Val Ile Ser Leu Glu Ser Gly Asp Ala Ser Ile His Asp Thr Val Glu 
            100                 105                 110         


Asn Leu Ile Ile Leu Ala Asn Asn Ser Leu Ser Ser Asn Gly Asn Val 
        115                 120                 125             


Thr Glu Ser Gly Cys Lys Glu Cys Glu Glu Leu Glu Glu Lys Asn Ile 
    130                 135                 140                 


Lys Glu Phe Leu Gln Ser Phe Val His Ile Val Gln Met Phe Ile Asn 
145                 150                 155                 160 


Thr Ser 
        


<210>  28
<211>  98
<212>  PRT
<213>  Homo sapiens

<400>  28

Met Ala Leu Leu Leu Ala Leu Ser Leu Leu Val Leu Trp Thr Ser Pro 
1               5                   10                  15      


Ala Pro Thr Leu Ser Gly Thr Asn Asp Ala Glu Asp Cys Cys Leu Ser 
            20                  25                  30          


Val Thr Gln Lys Pro Ile Pro Gly Tyr Ile Val Arg Asn Phe His Tyr 
        35                  40                  45              


Leu Leu Ile Lys Asp Gly Cys Arg Val Pro Ala Val Val Phe Thr Thr 
    50                  55                  60                  


Leu Arg Gly Arg Gln Leu Cys Ala Pro Pro Asp Gln Pro Trp Val Glu 
65                  70                  75                  80  


Arg Ile Ile Gln Arg Leu Gln Arg Thr Ser Ala Lys Met Lys Arg Arg 
                85                  90                  95      


Ser Ser 
        


<210>  29
<211>  93
<212>  PRT
<213>  Homo sapiens

<400>  29

Met Asn Ala Lys Val Val Val Val Leu Val Leu Val Leu Thr Ala Leu 
1               5                   10                  15      


Cys Leu Ser Asp Gly Lys Pro Val Ser Leu Ser Tyr Arg Cys Pro Cys 
            20                  25                  30          


Arg Phe Phe Glu Ser His Val Ala Arg Ala Asn Val Lys His Leu Lys 
        35                  40                  45              


Ile Leu Asn Thr Pro Asn Cys Ala Leu Gln Ile Val Ala Arg Leu Lys 
    50                  55                  60                  


Asn Asn Asn Arg Gln Val Cys Ile Asp Pro Lys Leu Lys Trp Ile Gln 
65                  70                  75                  80  


Glu Tyr Leu Glu Lys Ala Leu Asn Lys Arg Phe Lys Met 
                85                  90              


<210>  30
<211>  10
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  multiple stop translational readthrough motif

<400>  30
ugaugacuag                                                              10


<210>  31
<211>  7
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  construct DNA sequence encoding a TRM

<400>  31
tgactag                                                                  7


<210>  32
<211>  7
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  construct DNA sequence encoding a TRM

<400>  32
tagctag                                                                  7


<210>  33
<211>  7
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  construct DNA sequence encoding a TRM

<400>  33
taactag                                                                  7


<210>  34
<211>  9
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  construct DNA sequence encoding a TRM

<400>  34
tgacaatta                                                                9


<210>  35
<211>  9
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  construct DNA sequence encoding a TRM

<400>  35
tagcaatta                                                                9


<210>  36
<211>  9
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  construct DNA sequence encoding a TRM

<400>  36
taacaatta                                                                9


<210>  37
<211>  9
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  construct DNA sequence encoding a TRM

<400>  37
tgactagca                                                                9


<210>  38
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  basic amino acid furin target sequence


<220>
<221>  misc_feature
<222>  (2)..(2)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  MISC_FEATURE
<222>  (3)..(3)
<223>  Xaa may be Arg or Lys

<400>  38

Arg Xaa Xaa Arg 
1               


<210>  39
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  consensus Tobacco Etch Virus (TEV) cleavage site

<400>  39

Glu Asn Leu Tyr Phe Gln Ser 
1               5           


<210>  40
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  motif involved in cleavage activity


<220>
<221>  misc_feature
<222>  (2)..(2)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (4)..(4)
<223>  Xaa can be any naturally occurring amino acid

<400>  40

Asp Xaa Glu Xaa Asn Pro Gly Pro 
1               5               


<210>  41
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  cleavage site motif

<400>  41

Asp Ile Glu Thr Asn Pro Gly Pro 
1               5               


<210>  42
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  cleavage site motif

<400>  42

Asp Val Glu Thr Asn Pro Gly Pro 
1               5               


<210>  43
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  cleavage site motif

<400>  43

Asp Val Glu Met Asn Pro Gly Pro 
1               5               


<210>  44
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  cleavage site motif

<400>  44

Asp Val Glu Ser Asn Pro Gly Pro 
1               5               


<210>  45
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  cleavage site motif

<400>  45

Asp Met Glu Ser Asn Pro Gly Pro 
1               5               


<210>  46
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  cleavage site motif

<400>  46

Asp Val Glu Glu Asn Pro Gly Pro 
1               5               


<210>  47
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  cleavage site motif

<400>  47

Asp Ile Glu Leu Asn Pro Gly Pro 
1               5               


<210>  48
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  cleavage site motif

<400>  48

Asp Ile Glu Gln Asn Pro Gly Pro 
1               5               


<210>  49
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  cleavage site motif

<400>  49

Asp Ser Glu Phe Asn Pro Gly Pro 
1               5               


<210>  50
<211>  19
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  cleavage sequence

<400>  50

Leu Leu Asn Phe Asp Leu Leu Lys Leu Ala Gly Asp Val Glu Ser Asn 
1               5                   10                  15      


Pro Gly Pro 
            


<210>  51
<211>  19
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  cleavage sequence

<400>  51

Leu Leu Asn Phe Asp Leu Leu Lys Leu Ala Gly Asp Val Gln Ser Asn 
1               5                   10                  15      


Pro Gly Pro 
            


<210>  52
<211>  19
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  cleavage sequence

<400>  52

Leu Leu Asn Phe Asp Leu Leu Lys Leu Ala Gly Asp Val Glu Ile Asn 
1               5                   10                  15      


Pro Gly Pro 
            


<210>  53
<211>  19
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  cleavage sequence

<400>  53

Leu Leu Asn Phe Asp Leu Leu Lys Leu Ala Gly Asp Val Glu Phe Asn 
1               5                   10                  15      


Pro Gly Pro 
            


<210>  54
<211>  19
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  cleavage sequence

<400>  54

Leu Leu Asn Phe Asp Leu Leu Lys Leu Ala Gly Asp Val Glu Ser His 
1               5                   10                  15      


Pro Gly Pro 
            


<210>  55
<211>  19
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  cleavage sequence

<400>  55

Leu Leu Asn Phe Asp Leu Leu Lys Leu Ala Gly Asp Val Glu Ser Glu 
1               5                   10                  15      


Pro Gly Pro 
            


<210>  56
<211>  19
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  cleavage sequence

<400>  56

Leu Leu Asn Phe Asp Leu Leu Lys Leu Ala Gly Asp Val Glu Ser Gln 
1               5                   10                  15      


Pro Gly Pro 
            


<210>  57
<211>  19
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  cleavage sequence

<400>  57

Leu Leu Asn Phe Asp Leu Leu Lys Leu Ala Gly Asp Val Glu Ser Asn 
1               5                   10                  15      


Pro Gly Gly 
            


<210>  58
<211>  20
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  cleavage site, 2A-like sequence

<400>  58

Tyr His Ala Asp Tyr Tyr Lys Gln Arg Leu Ile His Asp Val Glu Met 
1               5                   10                  15      


Asn Pro Gly Pro 
            20  


<210>  59
<211>  20
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  cleavage site, 2A-like sequence

<400>  59

His Tyr Ala Gly Tyr Phe Ala Asp Leu Leu Ile His Asp Ile Glu Thr 
1               5                   10                  15      


Asn Pro Gly Pro 
            20  


<210>  60
<211>  20
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  cleavage site, 2A-like sequence

<400>  60

Gln Cys Thr Asn Tyr Ala Leu Leu Lys Leu Ala Gly Asp Val Glu Ser 
1               5                   10                  15      


Asn Pro Gly Pro 
            20  


<210>  61
<211>  19
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  cleavage site, 2A-like sequence

<400>  61

Ala Thr Asn Phe Ser Leu Leu Lys Gln Ala Gly Asp Val Glu Glu Asn 
1               5                   10                  15      


Pro Gly Pro 
            


<210>  62
<211>  19
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  cleavage site, 2A-like sequence

<400>  62

Ala Ala Arg Gln Met Leu Leu Leu Leu Ser Gly Asp Val Glu Thr Asn 
1               5                   10                  15      


Pro Gly Pro 
            


<210>  63
<211>  20
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  cleavage site, 2A-like sequence

<400>  63

Arg Ala Glu Gly Arg Gly Ser Leu Leu Thr Cys Gly Asp Val Glu Glu 
1               5                   10                  15      


Asn Pro Gly Pro 
            20  


<210>  64
<211>  20
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  cleavage site, 2A-like sequence

<400>  64

Thr Arg Ala Glu Ile Glu Asp Glu Leu Ile Arg Ala Gly Ile Glu Ser 
1               5                   10                  15      


Asn Pro Gly Pro 
            20  


<210>  65
<211>  20
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  cleavage site, 2A-like sequence

<400>  65

Thr Arg Ala Glu Ile Glu Asp Glu Leu Ile Arg Ala Asp Ile Glu Ser 
1               5                   10                  15      


Asn Pro Gly Pro 
            20  


<210>  66
<211>  20
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  cleavage site, 2A-like sequence

<400>  66

Ala Lys Phe Gln Ile Asp Lys Ile Leu Ile Ser Gly Asp Val Glu Leu 
1               5                   10                  15      


Asn Pro Gly Pro 
            20  


<210>  67
<211>  20
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  cleavage site, 2A-like sequence

<400>  67

Ser Ser Ile Ile Arg Thr Lys Met Leu Val Ser Gly Asp Val Glu Glu 
1               5                   10                  15      


Asn Pro Gly Pro 
            20  


<210>  68
<211>  20
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  cleavage site, 2A-like sequence

<400>  68

Cys Asp Ala Gln Arg Gln Lys Leu Leu Leu Ser Gly Asp Ile Glu Gln 
1               5                   10                  15      


Asn Pro Gly Pro 
            20  


<210>  69
<211>  20
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  cleavage site, 2A-like sequence

<400>  69

Tyr Pro Ile Asp Phe Gly Gly Phe Leu Val Lys Ala Asp Ser Glu Phe 
1               5                   10                  15      


Asn Pro Gly Pro 
            20  


<210>  70
<211>  18
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  2A peptide from Thosea asigna virus capsid protein

<400>  70

Glu Gly Arg Gly Ser Leu Leu Thr Cys Gly Asp Val Glu Glu Asn Pro 
1               5                   10                  15      


Gly Pro 
        


<210>  71
<211>  57
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  cleavage sequence

<400>  71

Val Thr Glu Leu Leu Tyr Arg Met Lys Arg Ala Glu Thr Tyr Cys Pro 
1               5                   10                  15      


Arg Pro Leu Ala Ile His Pro Thr Glu Ala Arg His Lys Gln Lys Ile 
            20                  25                  30          


Val Ala Pro Val Lys Gln Thr Leu Asn Phe Asp Leu Leu Lys Leu Ala 
        35                  40                  45              


Gly Asp Val Glu Ser Asn Pro Gly Pro 
    50                  55          


<210>  72
<211>  40
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  cleavage sequence

<400>  72

Leu Leu Ala Ile His Pro Thr Glu Ala Arg His Lys Gln Lys Ile Val 
1               5                   10                  15      


Ala Pro Val Lys Gln Thr Leu Asn Phe Asp Leu Leu Lys Leu Ala Gly 
            20                  25                  30          


Asp Val Glu Ser Asn Pro Gly Pro 
        35                  40  


<210>  73
<211>  33
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  cleavage sequence

<400>  73

Glu Ala Arg His Lys Gln Lys Ile Val Ala Pro Val Lys Gln Thr Leu 
1               5                   10                  15      


Asn Phe Asp Leu Leu Lys Leu Ala Gly Asp Val Glu Ser Asn Pro Gly 
            20                  25                  30          


Pro 
    


<210>  74
<211>  24
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  cleavage sequence

<400>  74

Ala Pro Val Lys Gln Thr Leu Asn Phe Asp Leu Leu Lys Leu Ala Gly 
1               5                   10                  15      


Asp Val Glu Ser Asn Pro Gly Pro 
            20                  


<210>  75
<211>  118
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Humanised KM666 VH sequence

<400>  75

Gln Val Gln Leu Gln Glu Ser Gly Pro Gly Leu Val Lys Pro Ser Gln 
1               5                   10                  15      


Thr Leu Ser Ile Thr Cys Thr Val Ser Gly Phe Ser Leu Ala Ser Tyr 
            20                  25                  30          


Asn Ile His Trp Val Arg Gln Pro Pro Gly Lys Gly Leu Glu Trp Leu 
        35                  40                  45              


Gly Val Ile Trp Ala Gly Gly Ser Thr Asn Tyr Asn Ser Ala Leu Met 
    50                  55                  60                  


Ser Arg Leu Thr Ile Ser Lys Asp Asn Ser Lys Asn Gln Val Phe Leu 
65                  70                  75                  80  


Lys Met Ser Ser Leu Thr Ala Ala Asp Thr Ala Val Tyr Tyr Cys Ala 
                85                  90                  95      


Lys Arg Ser Asp Asp Tyr Ser Trp Phe Ala Tyr Trp Gly Gln Gly Thr 
            100                 105                 110         


Leu Val Thr Val Ser Ser 
        115             


<210>  76
<211>  108
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Humanised KM666 VL sequence

<400>  76

Glu Asn Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly 
1               5                   10                  15      


Asp Arg Val Thr Met Thr Cys Arg Ala Ser Ser Ser Val Ser Ser Ser 
            20                  25                  30          


Tyr Leu His Trp Tyr Gln Gln Lys Ser Gly Lys Ala Pro Lys Val Trp 
        35                  40                  45              


Ile Tyr Ser Thr Ser Asn Leu Ala Ser Gly Val Pro Ser Arg Phe Ser 
    50                  55                  60                  


Gly Ser Gly Ser Gly Thr Asp Tyr Thr Leu Thr Ile Ser Ser Leu Gln 
65                  70                  75                  80  


Pro Glu Asp Phe Ala Thr Tyr Tyr Cys Gln Gln Tyr Ser Gly Tyr Pro 
                85                  90                  95      


Ile Thr Phe Gly Gln Gly Thr Lys Val Glu Ile Lys 
            100                 105             


<210>  77
<211>  119
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Humanised 7A12 VH sequence

<400>  77

Gln Val Gln Leu Val Gln Ser Gly Ala Glu Val Lys Lys Pro Gly Ala 
1               5                   10                  15      


Ser Val Lys Val Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Ser Ser 
            20                  25                  30          


Trp Met Asn Trp Val Arg Gln Ala Pro Gly Gln Gly Leu Glu Trp Met 
        35                  40                  45              


Gly Arg Ile Tyr Pro Gly Asp Gly Asp Thr Asn Tyr Ala Gln Lys Phe 
    50                  55                  60                  


Gln Gly Arg Val Thr Met Thr Arg Asp Thr Ser Ile Ser Thr Ala Tyr 
65                  70                  75                  80  


Met Glu Leu Ser Arg Leu Arg Ser Asp Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Gly Thr Gly Tyr Leu Trp Tyr Phe Asp Val Trp Gly Gln Gly 
            100                 105                 110         


Thr Met Val Thr Val Ser Ser 
        115                 


<210>  78
<211>  108
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Humanised 7A12 VL sequence

<400>  78

Glu Ile Val Leu Thr Gln Ser Pro Ala Thr Leu Ser Leu Ser Pro Gly 
1               5                   10                  15      


Glu Arg Ala Thr Leu Ser Cys Arg Ala Ser Gln Asp Ile Asn Glu Asn 
            20                  25                  30          


Leu Ala Trp Tyr Gln Gln Lys Pro Gly Gln Ala Pro Arg Leu Leu Ile 
        35                  40                  45              


Tyr Tyr Thr Ser Asn Arg Ala Thr Gly Ile Pro Ala Arg Phe Ser Gly 
    50                  55                  60                  


Ser Gly Ser Gly Arg Asp Phe Thr Leu Thr Ile Ser Ser Leu Glu Pro 
65                  70                  75                  80  


Glu Asp Phe Ala Val Tyr Tyr Cys Gln Gln Tyr Asp Asn Leu Pro Phe 
                85                  90                  95      


Thr Phe Gly Gln Gly Thr Lys Val Glu Ile Lys Arg 
            100                 105             


<210>  79
<211>  41
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CD28 endodomain

<400>  79

Arg Ser Lys Arg Ser Arg Leu Leu His Ser Asp Tyr Met Asn Met Thr 
1               5                   10                  15      


Pro Arg Arg Pro Gly Pro Thr Arg Lys His Tyr Gln Pro Tyr Ala Pro 
            20                  25                  30          


Pro Arg Asp Phe Ala Ala Tyr Arg Ser 
        35                  40      


<210>  80
<211>  112
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CD3z endodomain

<400>  80

Arg Val Lys Phe Ser Arg Ser Ala Asp Ala Pro Ala Tyr Gln Gln Gly 
1               5                   10                  15      


Gln Asn Gln Leu Tyr Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr 
            20                  25                  30          


Asp Val Leu Asp Lys Arg Arg Gly Arg Asp Pro Glu Met Gly Gly Lys 
        35                  40                  45              


Pro Arg Arg Lys Asn Pro Gln Glu Gly Leu Tyr Asn Glu Leu Gln Lys 
    50                  55                  60                  


Asp Lys Met Ala Glu Ala Tyr Ser Glu Ile Gly Met Lys Gly Glu Arg 
65                  70                  75                  80  


Arg Arg Gly Lys Gly His Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala 
                85                  90                  95      


Thr Lys Asp Thr Tyr Asp Ala Leu His Met Gln Ala Leu Pro Pro Arg 
            100                 105                 110         


<210>  81
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ITAM (immunoreceptor tyrosine-based activation motif)


<220>
<221>  misc_feature
<222>  (2)..(3)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  MISC_FEATURE
<222>  (4)..(4)
<223>  Xaa may be Leu or Ile

<400>  81

Tyr Xaa Xaa Xaa 
1               


<210>  82
<211>  214
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  SHP-1 SH2 complete domain

<400>  82

Met Val Arg Trp Phe His Arg Asp Leu Ser Gly Leu Asp Ala Glu Thr 
1               5                   10                  15      


Leu Leu Lys Gly Arg Gly Val His Gly Ser Phe Leu Ala Arg Pro Ser 
            20                  25                  30          


Arg Lys Asn Gln Gly Asp Phe Ser Leu Ser Val Arg Val Gly Asp Gln 
        35                  40                  45              


Val Thr His Ile Arg Ile Gln Asn Ser Gly Asp Phe Tyr Asp Leu Tyr 
    50                  55                  60                  


Gly Gly Glu Lys Phe Ala Thr Leu Thr Glu Leu Val Glu Tyr Tyr Thr 
65                  70                  75                  80  


Gln Gln Gln Gly Val Leu Gln Asp Arg Asp Gly Thr Ile Ile His Leu 
                85                  90                  95      


Lys Tyr Pro Leu Asn Cys Ser Asp Pro Thr Ser Glu Arg Trp Tyr His 
            100                 105                 110         


Gly His Met Ser Gly Gly Gln Ala Glu Thr Leu Leu Gln Ala Lys Gly 
        115                 120                 125             


Glu Pro Trp Thr Phe Leu Val Arg Glu Ser Leu Ser Gln Pro Gly Asp 
    130                 135                 140                 


Phe Val Leu Ser Val Leu Ser Asp Gln Pro Lys Ala Gly Pro Gly Ser 
145                 150                 155                 160 


Pro Leu Arg Val Thr His Ile Lys Val Met Cys Glu Gly Gly Arg Tyr 
                165                 170                 175     


Thr Val Gly Gly Leu Glu Thr Phe Asp Ser Leu Thr Asp Leu Val Glu 
            180                 185                 190         


His Phe Lys Lys Thr Gly Ile Glu Glu Ala Ser Gly Ala Phe Val Tyr 
        195                 200                 205             


Leu Arg Gln Pro Tyr Tyr 
    210                 


<210>  83
<211>  97
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  SHP-1 SH2 domain 1

<400>  83

Trp Phe His Arg Asp Leu Ser Gly Leu Asp Ala Glu Thr Leu Leu Lys 
1               5                   10                  15      


Gly Arg Gly Val His Gly Ser Phe Leu Ala Arg Pro Ser Arg Lys Asn 
            20                  25                  30          


Gln Gly Asp Phe Ser Leu Ser Val Arg Val Gly Asp Gln Val Thr His 
        35                  40                  45              


Ile Arg Ile Gln Asn Ser Gly Asp Phe Tyr Asp Leu Tyr Gly Gly Glu 
    50                  55                  60                  


Lys Phe Ala Thr Leu Thr Glu Leu Val Glu Tyr Tyr Thr Gln Gln Gln 
65                  70                  75                  80  


Gly Val Leu Gln Asp Arg Asp Gly Thr Ile Ile His Leu Lys Tyr Pro 
                85                  90                  95      


Leu 
    


<210>  84
<211>  104
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  SHP-1 SH2 domain 2

<400>  84

Trp Tyr His Gly His Met Ser Gly Gly Gln Ala Glu Thr Leu Leu Gln 
1               5                   10                  15      


Ala Lys Gly Glu Pro Trp Thr Phe Leu Val Arg Glu Ser Leu Ser Gln 
            20                  25                  30          


Pro Gly Asp Phe Val Leu Ser Val Leu Ser Asp Gln Pro Lys Ala Gly 
        35                  40                  45              


Pro Gly Ser Pro Leu Arg Val Thr His Ile Lys Val Met Cys Glu Gly 
    50                  55                  60                  


Gly Arg Tyr Thr Val Gly Gly Leu Glu Thr Phe Asp Ser Leu Thr Asp 
65                  70                  75                  80  


Leu Val Glu His Phe Lys Lys Thr Gly Ile Glu Glu Ala Ser Gly Ala 
                85                  90                  95      


Phe Val Tyr Leu Arg Gln Pro Tyr 
            100                 


<210>  85
<211>  97
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  SHP-2 first SH2 domain

<400>  85

Trp Phe His Pro Asn Ile Thr Gly Val Glu Ala Glu Asn Leu Leu Leu 
1               5                   10                  15      


Thr Arg Gly Val Asp Gly Ser Phe Leu Ala Arg Pro Ser Lys Ser Asn 
            20                  25                  30          


Pro Gly Asp Phe Thr Leu Ser Val Arg Arg Asn Gly Ala Val Thr His 
        35                  40                  45              


Ile Lys Ile Gln Asn Thr Gly Asp Tyr Tyr Asp Leu Tyr Gly Gly Glu 
    50                  55                  60                  


Lys Phe Ala Thr Leu Ala Glu Leu Val Gln Tyr Tyr Met Glu His His 
65                  70                  75                  80  


Gly Gln Leu Lys Glu Lys Asn Gly Asp Val Ile Glu Leu Lys Tyr Pro 
                85                  90                  95      


Leu 
    


<210>  86
<211>  105
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  SHP-2 second SH2 domain

<400>  86

Trp Phe His Gly His Leu Ser Gly Lys Glu Ala Glu Lys Leu Leu Thr 
1               5                   10                  15      


Glu Lys Gly Lys His Gly Ser Phe Leu Val Arg Glu Ser Gln Ser His 
            20                  25                  30          


Pro Gly Asp Phe Val Leu Ser Val Arg Thr Gly Asp Asp Lys Gly Glu 
        35                  40                  45              


Ser Asn Asp Gly Lys Ser Lys Val Thr His Val Met Ile Arg Cys Gln 
    50                  55                  60                  


Glu Leu Lys Tyr Asp Val Gly Gly Gly Glu Arg Phe Asp Ser Leu Thr 
65                  70                  75                  80  


Asp Leu Val Glu His Tyr Lys Lys Asn Pro Met Val Glu Thr Leu Gly 
                85                  90                  95      


Thr Val Leu Gln Leu Lys Gln Pro Leu 
            100                 105 


<210>  87
<211>  211
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  SHP-2 tandem SH2 domains

<400>  87

Trp Phe His Pro Asn Ile Thr Gly Val Glu Ala Glu Asn Leu Leu Leu 
1               5                   10                  15      


Thr Arg Gly Val Asp Gly Ser Phe Leu Ala Arg Pro Ser Lys Ser Asn 
            20                  25                  30          


Pro Gly Asp Phe Thr Leu Ser Val Arg Arg Asn Gly Ala Val Thr His 
        35                  40                  45              


Ile Lys Ile Gln Asn Thr Gly Asp Tyr Tyr Asp Leu Tyr Gly Gly Glu 
    50                  55                  60                  


Lys Phe Ala Thr Leu Ala Glu Leu Val Gln Tyr Tyr Met Glu His His 
65                  70                  75                  80  


Gly Gln Leu Lys Glu Lys Asn Gly Asp Val Ile Glu Leu Lys Tyr Pro 
                85                  90                  95      


Leu Asn Cys Ala Asp Pro Thr Ser Glu Arg Trp Phe His Gly His Leu 
            100                 105                 110         


Ser Gly Lys Glu Ala Glu Lys Leu Leu Thr Glu Lys Gly Lys His Gly 
        115                 120                 125             


Ser Phe Leu Val Arg Glu Ser Gln Ser His Pro Gly Asp Phe Val Leu 
    130                 135                 140                 


Ser Val Arg Thr Gly Asp Asp Lys Gly Glu Ser Asn Asp Gly Lys Ser 
145                 150                 155                 160 


Lys Val Thr His Val Met Ile Arg Cys Gln Glu Leu Lys Tyr Asp Val 
                165                 170                 175     


Gly Gly Gly Glu Arg Phe Asp Ser Leu Thr Asp Leu Val Glu His Tyr 
            180                 185                 190         


Lys Lys Asn Pro Met Val Glu Thr Leu Gly Thr Val Leu Gln Leu Lys 
        195                 200                 205             


Gln Pro Leu 
    210     


<210>  88
<211>  177
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  dominant negative TGFbeta receptor II

<400>  88

Thr Ile Pro Pro His Val Gln Lys Ser Val Asn Asn Asp Met Ile Val 
1               5                   10                  15      


Thr Asp Asn Asn Gly Ala Val Lys Phe Pro Gln Leu Cys Lys Phe Cys 
            20                  25                  30          


Asp Val Arg Phe Ser Thr Cys Asp Asn Gln Lys Ser Cys Met Ser Asn 
        35                  40                  45              


Cys Ser Ile Thr Ser Ile Cys Glu Lys Pro Gln Glu Val Cys Val Ala 
    50                  55                  60                  


Val Trp Arg Lys Asn Asp Glu Asn Ile Thr Leu Glu Thr Val Cys His 
65                  70                  75                  80  


Asp Pro Lys Leu Pro Tyr His Asp Phe Ile Leu Glu Asp Ala Ala Ser 
                85                  90                  95      


Pro Lys Cys Ile Met Lys Glu Lys Lys Lys Pro Gly Glu Thr Phe Phe 
            100                 105                 110         


Met Cys Ser Cys Ser Ser Asp Glu Cys Asn Asp Asn Ile Ile Phe Ser 
        115                 120                 125             


Glu Glu Tyr Asn Thr Ser Asn Pro Asp Leu Leu Leu Val Ile Phe Gln 
    130                 135                 140                 


Val Thr Gly Ile Ser Leu Leu Pro Pro Leu Gly Val Ala Ile Ser Val 
145                 150                 155                 160 


Ile Ile Ile Phe Tyr Cys Tyr Arg Val Asn Arg Gln Gln Lys Leu Ser 
                165                 170                 175     


Ser 
    


<210>  89
<211>  107
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Human Ig Kappa constant domain

<400>  89

Arg Thr Val Ala Ala Pro Ser Val Phe Ile Phe Pro Pro Ser Asp Glu 
1               5                   10                  15      


Gln Leu Lys Ser Gly Thr Ala Ser Val Val Cys Leu Leu Asn Asn Phe 
            20                  25                  30          


Tyr Pro Arg Glu Ala Lys Val Gln Trp Lys Val Asp Asn Ala Leu Gln 
        35                  40                  45              


Ser Gly Asn Ser Gln Glu Ser Val Thr Glu Gln Asp Ser Lys Asp Ser 
    50                  55                  60                  


Thr Tyr Ser Leu Ser Ser Thr Leu Thr Leu Ser Lys Ala Asp Tyr Glu 
65                  70                  75                  80  


Lys His Lys Val Tyr Ala Cys Glu Val Thr His Gln Gly Leu Ser Ser 
                85                  90                  95      


Pro Val Thr Lys Ser Phe Asn Arg Gly Glu Cys 
            100                 105         


<210>  90
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Human Hinge

<400>  90

Glu Pro Lys Ser Cys Asp Lys Thr His Thr Cys Pro Pro Cys Pro 
1               5                   10                  15  


<210>  91
<211>  97
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Human IgG1 CH1 domain

<400>  91

Ser Thr Lys Gly Pro Ser Val Phe Pro Leu Ala Pro Ser Ser Lys Ser 
1               5                   10                  15      


Thr Ser Gly Gly Thr Ala Ala Leu Gly Cys Leu Val Lys Asp Tyr Phe 
            20                  25                  30          


Pro Glu Pro Val Thr Val Ser Trp Asn Ser Gly Ala Leu Thr Ser Gly 
        35                  40                  45              


Val His Thr Phe Pro Ala Val Leu Gln Ser Ser Gly Leu Tyr Ser Leu 
    50                  55                  60                  


Ser Ser Val Val Thr Val Pro Ser Ser Ser Leu Gly Thr Gln Thr Tyr 
65                  70                  75                  80  


Ile Cys Asn Val Asn His Lys Pro Ser Asn Thr Lys Val Asp Lys Arg 
                85                  90                  95      


Val 
    


<210>  92
<211>  21
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Transmembrane domain from human IL2R common gamma chain

<400>  92

Val Val Ile Ser Val Gly Ser Met Gly Leu Ile Ile Ser Leu Leu Cys 
1               5                   10                  15      


Val Tyr Phe Trp Leu 
            20      


<210>  93
<211>  25
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Transmembrane domain from human IL-2R beta

<400>  93

Ile Pro Trp Leu Gly His Leu Leu Val Gly Leu Ser Gly Ala Phe Gly 
1               5                   10                  15      


Phe Ile Ile Leu Val Tyr Leu Leu Ile 
            20                  25  


<210>  94
<211>  25
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Transmembrane domain from human IL-7R alpha

<400>  94

Pro Ile Leu Leu Thr Ile Ser Ile Leu Ser Phe Phe Ser Val Ala Leu 
1               5                   10                  15      


Leu Val Ile Leu Ala Cys Val Leu Trp 
            20                  25  


<210>  95
<211>  26
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Transmembrane domain from Human GF-CSFR alpha

<400>  95

Asn Leu Gly Ser Val Tyr Ile Tyr Val Leu Leu Ile Val Gly Thr Leu 
1               5                   10                  15      


Val Cys Gly Ile Val Leu Gly Phe Leu Phe 
            20                  25      


<210>  96
<211>  17
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Transmembrane domain from Human GM-CSFR common beta chain

<400>  96

Val Leu Ala Leu Ile Val Ile Phe Leu Thr Ile Ala Val Leu Leu Ala 
1               5                   10                  15      


Leu 
    


<210>  97
<211>  86
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Endodomain from human IL2R common gamma chain

<400>  97

Glu Arg Thr Met Pro Arg Ile Pro Thr Leu Lys Asn Leu Glu Asp Leu 
1               5                   10                  15      


Val Thr Glu Tyr His Gly Asn Phe Ser Ala Trp Ser Gly Val Ser Lys 
            20                  25                  30          


Gly Leu Ala Glu Ser Leu Gln Pro Asp Tyr Ser Glu Arg Leu Cys Leu 
        35                  40                  45              


Val Ser Glu Ile Pro Pro Lys Gly Gly Ala Leu Gly Glu Gly Pro Gly 
    50                  55                  60                  


Ala Ser Pro Cys Asn Gln His Ser Pro Tyr Trp Ala Pro Pro Cys Tyr 
65                  70                  75                  80  


Thr Leu Lys Pro Glu Thr 
                85      


<210>  98
<211>  286
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Endodomain from human IL-2R beta

<400>  98

Asn Cys Arg Asn Thr Gly Pro Trp Leu Lys Lys Val Leu Lys Cys Asn 
1               5                   10                  15      


Thr Pro Asp Pro Ser Lys Phe Phe Ser Gln Leu Ser Ser Glu His Gly 
            20                  25                  30          


Gly Asp Val Gln Lys Trp Leu Ser Ser Pro Phe Pro Ser Ser Ser Phe 
        35                  40                  45              


Ser Pro Gly Gly Leu Ala Pro Glu Ile Ser Pro Leu Glu Val Leu Glu 
    50                  55                  60                  


Arg Asp Lys Val Thr Gln Leu Leu Leu Gln Gln Asp Lys Val Pro Glu 
65                  70                  75                  80  


Pro Ala Ser Leu Ser Ser Asn His Ser Leu Thr Ser Cys Phe Thr Asn 
                85                  90                  95      


Gln Gly Tyr Phe Phe Phe His Leu Pro Asp Ala Leu Glu Ile Glu Ala 
            100                 105                 110         


Cys Gln Val Tyr Phe Thr Tyr Asp Pro Tyr Ser Glu Glu Asp Pro Asp 
        115                 120                 125             


Glu Gly Val Ala Gly Ala Pro Thr Gly Ser Ser Pro Gln Pro Leu Gln 
    130                 135                 140                 


Pro Leu Ser Gly Glu Asp Asp Ala Tyr Cys Thr Phe Pro Ser Arg Asp 
145                 150                 155                 160 


Asp Leu Leu Leu Phe Ser Pro Ser Leu Leu Gly Gly Pro Ser Pro Pro 
                165                 170                 175     


Ser Thr Ala Pro Gly Gly Ser Gly Ala Gly Glu Glu Arg Met Pro Pro 
            180                 185                 190         


Ser Leu Gln Glu Arg Val Pro Arg Asp Trp Asp Pro Gln Pro Leu Gly 
        195                 200                 205             


Pro Pro Thr Pro Gly Val Pro Asp Leu Val Asp Phe Gln Pro Pro Pro 
    210                 215                 220                 


Glu Leu Val Leu Arg Glu Ala Gly Glu Glu Val Pro Asp Ala Gly Pro 
225                 230                 235                 240 


Arg Glu Gly Val Ser Phe Pro Trp Ser Arg Pro Pro Gly Gln Gly Glu 
                245                 250                 255     


Phe Arg Ala Leu Asn Ala Arg Leu Pro Leu Asn Thr Asp Ala Tyr Leu 
            260                 265                 270         


Ser Leu Gln Glu Leu Gln Gly Gln Asp Pro Thr His Leu Val 
        275                 280                 285     


<210>  99
<211>  195
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Endodomain from human IL-7R alpha

<400>  99

Lys Lys Arg Ile Lys Pro Ile Val Trp Pro Ser Leu Pro Asp His Lys 
1               5                   10                  15      


Lys Thr Leu Glu His Leu Cys Lys Lys Pro Arg Lys Asn Leu Asn Val 
            20                  25                  30          


Ser Phe Asn Pro Glu Ser Phe Leu Asp Cys Gln Ile His Arg Val Asp 
        35                  40                  45              


Asp Ile Gln Ala Arg Asp Glu Val Glu Gly Phe Leu Gln Asp Thr Phe 
    50                  55                  60                  


Pro Gln Gln Leu Glu Glu Ser Glu Lys Gln Arg Leu Gly Gly Asp Val 
65                  70                  75                  80  


Gln Ser Pro Asn Cys Pro Ser Glu Asp Val Val Ile Thr Pro Glu Ser 
                85                  90                  95      


Phe Gly Arg Asp Ser Ser Leu Thr Cys Leu Ala Gly Asn Val Ser Ala 
            100                 105                 110         


Cys Asp Ala Pro Ile Leu Ser Ser Ser Arg Ser Leu Asp Cys Arg Glu 
        115                 120                 125             


Ser Gly Lys Asn Gly Pro His Val Tyr Gln Asp Leu Leu Leu Ser Leu 
    130                 135                 140                 


Gly Thr Thr Asn Ser Thr Leu Pro Pro Pro Phe Ser Leu Gln Ser Gly 
145                 150                 155                 160 


Ile Leu Thr Leu Asn Pro Val Ala Gln Gly Gln Pro Ile Leu Thr Ser 
                165                 170                 175     


Leu Gly Ser Asn Gln Glu Glu Ala Tyr Val Thr Met Ser Ser Phe Tyr 
            180                 185                 190         


Gln Asn Gln 
        195 


<210>  100
<211>  54
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Endodomain Derived from Human GM-CSFR alpha

<400>  100

Lys Arg Phe Leu Arg Ile Gln Arg Leu Phe Pro Pro Val Pro Gln Ile 
1               5                   10                  15      


Lys Asp Lys Leu Asn Asp Asn His Glu Val Glu Asp Glu Ile Ile Trp 
            20                  25                  30          


Glu Glu Phe Thr Pro Glu Glu Gly Lys Gly Tyr Arg Glu Glu Val Leu 
        35                  40                  45              


Thr Val Lys Glu Ile Thr 
    50                  


<210>  101
<211>  437
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Endodomain from GM-CSFR common beta chain

<400>  101

Arg Phe Cys Gly Ile Tyr Gly Tyr Arg Leu Arg Arg Lys Trp Glu Glu 
1               5                   10                  15      


Lys Ile Pro Asn Pro Ser Lys Ser His Leu Phe Gln Asn Gly Ser Ala 
            20                  25                  30          


Glu Leu Trp Pro Pro Gly Ser Met Ser Ala Phe Thr Ser Gly Ser Pro 
        35                  40                  45              


Pro His Gln Gly Pro Trp Gly Ser Arg Phe Pro Glu Leu Glu Gly Val 
    50                  55                  60                  


Phe Pro Val Gly Phe Gly Asp Ser Glu Val Ser Pro Leu Thr Ile Glu 
65                  70                  75                  80  


Asp Pro Lys His Val Cys Asp Pro Pro Ser Gly Pro Asp Thr Thr Pro 
                85                  90                  95      


Ala Ala Ser Asp Leu Pro Thr Glu Gln Pro Pro Ser Pro Gln Pro Gly 
            100                 105                 110         


Pro Pro Ala Ala Ser His Thr Pro Glu Lys Gln Ala Ser Ser Phe Asp 
        115                 120                 125             


Phe Asn Gly Pro Tyr Leu Gly Pro Pro His Ser Arg Ser Leu Pro Asp 
    130                 135                 140                 


Ile Leu Gly Gln Pro Glu Pro Pro Gln Glu Gly Gly Ser Gln Lys Ser 
145                 150                 155                 160 


Pro Pro Pro Gly Ser Leu Glu Tyr Leu Cys Leu Pro Ala Gly Gly Gln 
                165                 170                 175     


Val Gln Leu Val Pro Leu Ala Gln Ala Met Gly Pro Gly Gln Ala Val 
            180                 185                 190         


Glu Val Glu Arg Arg Pro Ser Gln Gly Ala Ala Gly Ser Pro Ser Leu 
        195                 200                 205             


Glu Ser Gly Gly Gly Pro Ala Pro Pro Ala Leu Gly Pro Arg Val Gly 
    210                 215                 220                 


Gly Gln Asp Gln Lys Asp Ser Pro Val Ala Ile Pro Met Ser Ser Gly 
225                 230                 235                 240 


Asp Thr Glu Asp Pro Gly Val Ala Ser Gly Tyr Val Ser Ser Ala Asp 
                245                 250                 255     


Leu Val Phe Thr Pro Asn Ser Gly Ala Ser Ser Val Ser Leu Val Pro 
            260                 265                 270         


Ser Leu Gly Leu Pro Ser Asp Gln Thr Pro Ser Leu Cys Pro Gly Leu 
        275                 280                 285             


Ala Ser Gly Pro Pro Gly Ala Pro Gly Pro Val Lys Ser Gly Phe Glu 
    290                 295                 300                 


Gly Tyr Val Glu Leu Pro Pro Ile Glu Gly Arg Ser Pro Arg Ser Pro 
305                 310                 315                 320 


Arg Asn Asn Pro Val Pro Pro Glu Ala Lys Ser Pro Val Leu Asn Pro 
                325                 330                 335     


Gly Glu Arg Pro Ala Asp Val Ser Pro Thr Ser Pro Gln Pro Glu Gly 
            340                 345                 350         


Leu Leu Val Leu Gln Gln Val Gly Asp Tyr Cys Phe Leu Pro Gly Leu 
        355                 360                 365             


Gly Pro Gly Pro Leu Ser Leu Arg Ser Lys Pro Ser Ser Pro Gly Pro 
    370                 375                 380                 


Gly Pro Glu Ile Lys Asn Leu Asp Gln Ala Phe Gln Val Lys Lys Pro 
385                 390                 395                 400 


Pro Gly Gln Ala Val Pro Gln Val Pro Val Ile Gln Leu Phe Lys Ala 
                405                 410                 415     


Leu Lys Gln Gln Asp Tyr Leu Ser Leu Pro Pro Trp Glu Val Asn Lys 
            420                 425                 430         


Pro Gly Glu Val Cys 
        435         


<210>  102
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CXCL12 loop region motif

<400>  102

Arg Phe Phe Glu Ser His 
1               5       


<210>  103
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  cleavage site motif

<400>  103

Asp Val Glu Leu Asn Pro Gly Pro 
1               5               


<210>  104
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Mucin 1 motif

<400>  104

Pro Asp Thr Arg 
1               


