                         SEQUENCE LISTING

<110>  Ospedale San Raffaele S.r.l.
       Fondazione Telethon
 
<120>  EPIDERMAL GROWTH FACTOR RECEPTOR

<130>  P120279PCT

<140>  PCT/EP2021/062873
<141>  2021-05-14

<150>  GB 2007169.2
<151>  2020-05-14

<160>  44    

<170>  PatentIn version 3.5

<210>  1
<211>  1210
<212>  PRT
<213>  Homo sapiens

<400>  1

Met Arg Pro Ser Gly Thr Ala Gly Ala Ala Leu Leu Ala Leu Leu Ala 
1               5                   10                  15      


Ala Leu Cys Pro Ala Ser Arg Ala Leu Glu Glu Lys Lys Val Cys Gln 
            20                  25                  30          


Gly Thr Ser Asn Lys Leu Thr Gln Leu Gly Thr Phe Glu Asp His Phe 
        35                  40                  45              


Leu Ser Leu Gln Arg Met Phe Asn Asn Cys Glu Val Val Leu Gly Asn 
    50                  55                  60                  


Leu Glu Ile Thr Tyr Val Gln Arg Asn Tyr Asp Leu Ser Phe Leu Lys 
65                  70                  75                  80  


Thr Ile Gln Glu Val Ala Gly Tyr Val Leu Ile Ala Leu Asn Thr Val 
                85                  90                  95      


Glu Arg Ile Pro Leu Glu Asn Leu Gln Ile Ile Arg Gly Asn Met Tyr 
            100                 105                 110         


Tyr Glu Asn Ser Tyr Ala Leu Ala Val Leu Ser Asn Tyr Asp Ala Asn 
        115                 120                 125             


Lys Thr Gly Leu Lys Glu Leu Pro Met Arg Asn Leu Gln Glu Ile Leu 
    130                 135                 140                 


His Gly Ala Val Arg Phe Ser Asn Asn Pro Ala Leu Cys Asn Val Glu 
145                 150                 155                 160 


Ser Ile Gln Trp Arg Asp Ile Val Ser Ser Asp Phe Leu Ser Asn Met 
                165                 170                 175     


Ser Met Asp Phe Gln Asn His Leu Gly Ser Cys Gln Lys Cys Asp Pro 
            180                 185                 190         


Ser Cys Pro Asn Gly Ser Cys Trp Gly Ala Gly Glu Glu Asn Cys Gln 
        195                 200                 205             


Lys Leu Thr Lys Ile Ile Cys Ala Gln Gln Cys Ser Gly Arg Cys Arg 
    210                 215                 220                 


Gly Lys Ser Pro Ser Asp Cys Cys His Asn Gln Cys Ala Ala Gly Cys 
225                 230                 235                 240 


Thr Gly Pro Arg Glu Ser Asp Cys Leu Val Cys Arg Lys Phe Arg Asp 
                245                 250                 255     


Glu Ala Thr Cys Lys Asp Thr Cys Pro Pro Leu Met Leu Tyr Asn Pro 
            260                 265                 270         


Thr Thr Tyr Gln Met Asp Val Asn Pro Glu Gly Lys Tyr Ser Phe Gly 
        275                 280                 285             


Ala Thr Cys Val Lys Lys Cys Pro Arg Asn Tyr Val Val Thr Asp His 
    290                 295                 300                 


Gly Ser Cys Val Arg Ala Cys Gly Ala Asp Ser Tyr Glu Met Glu Glu 
305                 310                 315                 320 


Asp Gly Val Arg Lys Cys Lys Lys Cys Glu Gly Pro Cys Arg Lys Val 
                325                 330                 335     


Cys Asn Gly Ile Gly Ile Gly Glu Phe Lys Asp Ser Leu Ser Ile Asn 
            340                 345                 350         


Ala Thr Asn Ile Lys His Phe Lys Asn Cys Thr Ser Ile Ser Gly Asp 
        355                 360                 365             


Leu His Ile Leu Pro Val Ala Phe Arg Gly Asp Ser Phe Thr His Thr 
    370                 375                 380                 


Pro Pro Leu Asp Pro Gln Glu Leu Asp Ile Leu Lys Thr Val Lys Glu 
385                 390                 395                 400 


Ile Thr Gly Phe Leu Leu Ile Gln Ala Trp Pro Glu Asn Arg Thr Asp 
                405                 410                 415     


Leu His Ala Phe Glu Asn Leu Glu Ile Ile Arg Gly Arg Thr Lys Gln 
            420                 425                 430         


His Gly Gln Phe Ser Leu Ala Val Val Ser Leu Asn Ile Thr Ser Leu 
        435                 440                 445             


Gly Leu Arg Ser Leu Lys Glu Ile Ser Asp Gly Asp Val Ile Ile Ser 
    450                 455                 460                 


Gly Asn Lys Asn Leu Cys Tyr Ala Asn Thr Ile Asn Trp Lys Lys Leu 
465                 470                 475                 480 


Phe Gly Thr Ser Gly Gln Lys Thr Lys Ile Ile Ser Asn Arg Gly Glu 
                485                 490                 495     


Asn Ser Cys Lys Ala Thr Gly Gln Val Cys His Ala Leu Cys Ser Pro 
            500                 505                 510         


Glu Gly Cys Trp Gly Pro Glu Pro Arg Asp Cys Val Ser Cys Arg Asn 
        515                 520                 525             


Val Ser Arg Gly Arg Glu Cys Val Asp Lys Cys Asn Leu Leu Glu Gly 
    530                 535                 540                 


Glu Pro Arg Glu Phe Val Glu Asn Ser Glu Cys Ile Gln Cys His Pro 
545                 550                 555                 560 


Glu Cys Leu Pro Gln Ala Met Asn Ile Thr Cys Thr Gly Arg Gly Pro 
                565                 570                 575     


Asp Asn Cys Ile Gln Cys Ala His Tyr Ile Asp Gly Pro His Cys Val 
            580                 585                 590         


Lys Thr Cys Pro Ala Gly Val Met Gly Glu Asn Asn Thr Leu Val Trp 
        595                 600                 605             


Lys Tyr Ala Asp Ala Gly His Val Cys His Leu Cys His Pro Asn Cys 
    610                 615                 620                 


Thr Tyr Gly Cys Thr Gly Pro Gly Leu Glu Gly Cys Pro Thr Asn Gly 
625                 630                 635                 640 


Pro Lys Ile Pro Ser Ile Ala Thr Gly Met Val Gly Ala Leu Leu Leu 
                645                 650                 655     


Leu Leu Val Val Ala Leu Gly Ile Gly Leu Phe Met Arg Arg Arg His 
            660                 665                 670         


Ile Val Arg Lys Arg Thr Leu Arg Arg Leu Leu Gln Glu Arg Glu Leu 
        675                 680                 685             


Val Glu Pro Leu Thr Pro Ser Gly Glu Ala Pro Asn Gln Ala Leu Leu 
    690                 695                 700                 


Arg Ile Leu Lys Glu Thr Glu Phe Lys Lys Ile Lys Val Leu Gly Ser 
705                 710                 715                 720 


Gly Ala Phe Gly Thr Val Tyr Lys Gly Leu Trp Ile Pro Glu Gly Glu 
                725                 730                 735     


Lys Val Lys Ile Pro Val Ala Ile Lys Glu Leu Arg Glu Ala Thr Ser 
            740                 745                 750         


Pro Lys Ala Asn Lys Glu Ile Leu Asp Glu Ala Tyr Val Met Ala Ser 
        755                 760                 765             


Val Asp Asn Pro His Val Cys Arg Leu Leu Gly Ile Cys Leu Thr Ser 
    770                 775                 780                 


Thr Val Gln Leu Ile Thr Gln Leu Met Pro Phe Gly Cys Leu Leu Asp 
785                 790                 795                 800 


Tyr Val Arg Glu His Lys Asp Asn Ile Gly Ser Gln Tyr Leu Leu Asn 
                805                 810                 815     


Trp Cys Val Gln Ile Ala Lys Gly Met Asn Tyr Leu Glu Asp Arg Arg 
            820                 825                 830         


Leu Val His Arg Asp Leu Ala Ala Arg Asn Val Leu Val Lys Thr Pro 
        835                 840                 845             


Gln His Val Lys Ile Thr Asp Phe Gly Leu Ala Lys Leu Leu Gly Ala 
    850                 855                 860                 


Glu Glu Lys Glu Tyr His Ala Glu Gly Gly Lys Val Pro Ile Lys Trp 
865                 870                 875                 880 


Met Ala Leu Glu Ser Ile Leu His Arg Ile Tyr Thr His Gln Ser Asp 
                885                 890                 895     


Val Trp Ser Tyr Gly Val Thr Val Trp Glu Leu Met Thr Phe Gly Ser 
            900                 905                 910         


Lys Pro Tyr Asp Gly Ile Pro Ala Ser Glu Ile Ser Ser Ile Leu Glu 
        915                 920                 925             


Lys Gly Glu Arg Leu Pro Gln Pro Pro Ile Cys Thr Ile Asp Val Tyr 
    930                 935                 940                 


Met Ile Met Val Lys Cys Trp Met Ile Asp Ala Asp Ser Arg Pro Lys 
945                 950                 955                 960 


Phe Arg Glu Leu Ile Ile Glu Phe Ser Lys Met Ala Arg Asp Pro Gln 
                965                 970                 975     


Arg Tyr Leu Val Ile Gln Gly Asp Glu Arg Met His Leu Pro Ser Pro 
            980                 985                 990         


Thr Asp Ser Asn Phe Tyr Arg Ala  Leu Met Asp Glu Glu  Asp Met Asp 
        995                 1000                 1005             


Asp Val  Val Asp Ala Asp Glu  Tyr Leu Ile Pro Gln  Gln Gly Phe 
    1010                 1015                 1020             


Phe Ser  Ser Pro Ser Thr Ser  Arg Thr Pro Leu Leu  Ser Ser Leu 
    1025                 1030                 1035             


Ser Ala  Thr Ser Asn Asn Ser  Thr Val Ala Cys Ile  Asp Arg Asn 
    1040                 1045                 1050             


Gly Leu  Gln Ser Cys Pro Ile  Lys Glu Asp Ser Phe  Leu Gln Arg 
    1055                 1060                 1065             


Tyr Ser  Ser Asp Pro Thr Gly  Ala Leu Thr Glu Asp  Ser Ile Asp 
    1070                 1075                 1080             


Asp Thr  Phe Leu Pro Val Pro  Glu Tyr Ile Asn Gln  Ser Val Pro 
    1085                 1090                 1095             


Lys Arg  Pro Ala Gly Ser Val  Gln Asn Pro Val Tyr  His Asn Gln 
    1100                 1105                 1110             


Pro Leu  Asn Pro Ala Pro Ser  Arg Asp Pro His Tyr  Gln Asp Pro 
    1115                 1120                 1125             


His Ser  Thr Ala Val Gly Asn  Pro Glu Tyr Leu Asn  Thr Val Gln 
    1130                 1135                 1140             


Pro Thr  Cys Val Asn Ser Thr  Phe Asp Ser Pro Ala  His Trp Ala 
    1145                 1150                 1155             


Gln Lys  Gly Ser His Gln Ile  Ser Leu Asp Asn Pro  Asp Tyr Gln 
    1160                 1165                 1170             


Gln Asp  Phe Phe Pro Lys Glu  Ala Lys Pro Asn Gly  Ile Phe Lys 
    1175                 1180                 1185             


Gly Ser  Thr Ala Glu Asn Ala  Glu Tyr Leu Arg Val  Ala Pro Gln 
    1190                 1195                 1200             


Ser Ser  Glu Phe Ile Gly Ala  
    1205                 1210 


<210>  2
<211>  335
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  example epidermal growth factor receptor (EGFRt) amino acid 
       sequence

<400>  2

Arg Lys Val Cys Asn Gly Ile Gly Ile Gly Glu Phe Lys Asp Ser Leu 
1               5                   10                  15      


Ser Ile Asn Ala Thr Asn Ile Lys His Phe Lys Asn Cys Thr Ser Ile 
            20                  25                  30          


Ser Gly Asp Leu His Ile Leu Pro Val Ala Phe Arg Gly Asp Ser Phe 
        35                  40                  45              


Thr His Thr Pro Pro Leu Asp Pro Gln Glu Leu Asp Ile Leu Lys Thr 
    50                  55                  60                  


Val Lys Glu Ile Thr Gly Phe Leu Leu Ile Gln Ala Trp Pro Glu Asn 
65                  70                  75                  80  


Arg Thr Asp Leu His Ala Phe Glu Asn Leu Glu Ile Ile Arg Gly Arg 
                85                  90                  95      


Thr Lys Gln His Gly Gln Phe Ser Leu Ala Val Val Ser Leu Asn Ile 
            100                 105                 110         


Thr Ser Leu Gly Leu Arg Ser Leu Lys Glu Ile Ser Asp Gly Asp Val 
        115                 120                 125             


Ile Ile Ser Gly Asn Lys Asn Leu Cys Tyr Ala Asn Thr Ile Asn Trp 
    130                 135                 140                 


Lys Lys Leu Phe Gly Thr Ser Gly Gln Lys Thr Lys Ile Ile Ser Asn 
145                 150                 155                 160 


Arg Gly Glu Asn Ser Cys Lys Ala Thr Gly Gln Val Cys His Ala Leu 
                165                 170                 175     


Cys Ser Pro Glu Gly Cys Trp Gly Pro Glu Pro Arg Asp Cys Val Ser 
            180                 185                 190         


Cys Arg Asn Val Ser Arg Gly Arg Glu Cys Val Asp Lys Cys Asn Leu 
        195                 200                 205             


Leu Glu Gly Glu Pro Arg Glu Phe Val Glu Asn Ser Glu Cys Ile Gln 
    210                 215                 220                 


Cys His Pro Glu Cys Leu Pro Gln Ala Met Asn Ile Thr Cys Thr Gly 
225                 230                 235                 240 


Arg Gly Pro Asp Asn Cys Ile Gln Cys Ala His Tyr Ile Asp Gly Pro 
                245                 250                 255     


His Cys Val Lys Thr Cys Pro Ala Gly Val Met Gly Glu Asn Asn Thr 
            260                 265                 270         


Leu Val Trp Lys Tyr Ala Asp Ala Gly His Val Cys His Leu Cys His 
        275                 280                 285             


Pro Asn Cys Thr Tyr Gly Cys Thr Gly Pro Gly Leu Glu Gly Cys Pro 
    290                 295                 300                 


Thr Asn Gly Pro Lys Ile Pro Ser Ile Ala Thr Gly Met Val Gly Ala 
305                 310                 315                 320 


Leu Leu Leu Leu Leu Val Val Ala Leu Gly Ile Gly Leu Phe Met 
                325                 330                 335 


<210>  3
<211>  1005
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  example nucleotide sequence encoding EGFRt

<400>  3
agaaaagtgt gcaacggcat cggcatcgga gagttcaagg acagcctgag catcaacgcc       60

accaacatca agcacttcaa gaactgcacc agcatcagcg gcgacctgca cattctgcct      120

gtggccttta gaggcgacag cttcacccac acacctccac tggatcccca agagctggac      180

atcctgaaaa ccgtgaaaga gatcaccgga tttctgttga tccaggcttg gcccgagaac      240

cggacagatc tgcacgcctt cgagaacctg gaaatcatca gaggccggac caagcagcac      300

ggccagtttt ctctggctgt ggtgtccctg aacatcacca gcctgggcct gagaagcctg      360

aaagaaatca gcgacggcga cgtgatcatc tccggcaaca agaacctgtg ctacgccaac      420

accatcaact ggaagaagct gttcggcacc agcggccaga aaacaaagat catcagcaac      480

cggggcgaga acagctgcaa ggctacaggc caagtgtgcc acgctctgtg tagccctgaa      540

ggctgttggg gacccgagcc tagagattgc gtgtcctgca gaaacgtgtc ccggggcaga      600

gaatgcgtgg acaagtgcaa tctgctggaa ggcgagcccc gcgagttcgt ggaaaacagc      660

gagtgcatcc agtgtcaccc cgagtgtctg ccccaggcca tgaacattac ctgtaccggc      720

agaggccccg acaactgtat tcagtgcgcc cactacatcg acggccctca ctgcgtgaaa      780

acatgtcctg ctggcgtgat gggagagaac aacaccctcg tgtggaagta tgccgacgcc      840

ggacatgtgt gccacctgtg tcaccctaat tgcacctacg gctgtacagg ccctggcctg      900

gaaggctgtc caacaaacgg acctaagatc ccctctatcg ccaccggcat ggttggagcc      960

ctgctgcttc tgctggtggt ggcccttgga atcggcctgt ttatg                     1005


<210>  4
<211>  28
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  example NGFR signal peptide

<400>  4

Met Gly Ala Gly Ala Thr Gly Arg Ala Met Asp Gly Pro Arg Leu Leu 
1               5                   10                  15      


Leu Leu Leu Leu Leu Gly Val Ser Leu Gly Gly Ala 
            20                  25              


<210>  5
<211>  84
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  example nucleotide sequence encoding an NGFR signal peptide

<400>  5
atgggagctg gtgctaccgg cagagctatg gatggaccta gactgctgct cctgctgctg       60

ctcggagttt ctcttggcgg agcc                                              84


<210>  6
<211>  22
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  example GMS SFR alpha signal peptide

<400>  6

Met Leu Leu Leu Val Thr Ser Leu Leu Leu Cys Glu Leu Pro His Pro 
1               5                   10                  15      


Ala Phe Leu Leu Ile Pro 
            20          


<210>  7
<211>  66
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  example GMS SFR alpha signal peptide

<400>  7
atgctgctgc tggtcacctc tctgctgctg tgcgaactgc cccatcctgc ctttctgctg       60

atcccc                                                                  66


<210>  8
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  example nucleotide sequence encoding a cytoplasmic tail

<400>  8
aagcggtgga accggggcat cctg                                              24


<210>  9
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  example nucleotide sequence encoding a cytoplasmic tail

<400>  9
cggcggagac acatcgtgcg gaag                                              24


<210>  10
<211>  23
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  epidermal growth factor receptor (EGFR) transmembrane domain

<400>  10

Ile Ala Thr Gly Met Val Gly Ala Leu Leu Leu Leu Leu Val Val Ala 
1               5                   10                  15      


Leu Gly Ile Gly Leu Phe Met 
            20              


<210>  11
<211>  69
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  nucleotide sequence encoding an EGFR transmembrane domain

<400>  11
atcgccaccg gcatggttgg agccctgctg cttctgctgg tggtggccct tggaatcggc       60

ctgtttatg                                                               69


<210>  12
<211>  22
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  NGFR transmembrane domain

<400>  12

Leu Ile Pro Val Tyr Cys Ser Ile Leu Ala Ala Val Val Val Gly Leu 
1               5                   10                  15      


Val Ala Tyr Ile Ala Phe 
            20          


<210>  13
<211>  66
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  nucleotide sequence encoding an NGFR transmembrane domain

<400>  13
ctgatccccg tgtactgtag catcctggcc gccgtggttg tgggactcgt ggcctatatc       60

gccttc                                                                  66


<210>  14
<211>  24
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  recycling signal sequence

<400>  14

Tyr Gln Pro Leu Ser Gln Ile Lys Arg Leu Leu Ser Asp Ser Phe Leu 
1               5                   10                  15      


Phe Asp Asn Pro Val Tyr Ala Ser 
            20                  


<210>  15
<211>  72
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  nucleotide sequence encoding a recycling signal

<400>  15
tatcagcctc tgagccagat caagcggctg ctgagcgact ccttcctgtt cgacaacccc       60

gtgtacgcta gc                                                           72


<210>  16
<211>  1089
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  nucleotide sequence encoding modified EGFRt (with NGFR signal 
       peptide)

<400>  16
atgggagctg gtgctaccgg cagagctatg gatggaccta gactgctgct cctgctgctg       60

ctcggagttt ctcttggcgg agccagaaaa gtgtgcaacg gcatcggcat cggagagttc      120

aaggacagcc tgagcatcaa cgccaccaac atcaagcact tcaagaactg caccagcatc      180

agcggcgacc tgcacattct gcctgtggcc tttagaggcg acagcttcac ccacacacct      240

ccactggatc cccaagagct ggacatcctg aaaaccgtga aagagatcac cggatttctg      300

ttgatccagg cttggcccga gaaccggaca gatctgcacg ccttcgagaa cctggaaatc      360

atcagaggcc ggaccaagca gcacggccag ttttctctgg ctgtggtgtc cctgaacatc      420

accagcctgg gcctgagaag cctgaaagaa atcagcgacg gcgacgtgat catctccggc      480

aacaagaacc tgtgctacgc caacaccatc aactggaaga agctgttcgg caccagcggc      540

cagaaaacaa agatcatcag caaccggggc gagaacagct gcaaggctac aggccaagtg      600

tgccacgctc tgtgtagccc tgaaggctgt tggggacccg agcctagaga ttgcgtgtcc      660

tgcagaaacg tgtcccgggg cagagaatgc gtggacaagt gcaatctgct ggaaggcgag      720

ccccgcgagt tcgtggaaaa cagcgagtgc atccagtgtc accccgagtg tctgccccag      780

gccatgaaca ttacctgtac cggcagaggc cccgacaact gtattcagtg cgcccactac      840

atcgacggcc ctcactgcgt gaaaacatgt cctgctggcg tgatgggaga gaacaacacc      900

ctcgtgtgga agtatgccga cgccggacat gtgtgccacc tgtgtcaccc taattgcacc      960

tacggctgta caggccctgg cctggaaggc tgtccaacaa acggacctaa gatcccctct     1020

atcgccaccg gcatggttgg agccctgctg cttctgctgg tggtggccct tggaatcggc     1080

ctgtttatg                                                             1089


<210>  17
<211>  363
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  modified EGFRt (with NGFR signal peptide)

<400>  17

Met Gly Ala Gly Ala Thr Gly Arg Ala Met Asp Gly Pro Arg Leu Leu 
1               5                   10                  15      


Leu Leu Leu Leu Leu Gly Val Ser Leu Gly Gly Ala Arg Lys Val Cys 
            20                  25                  30          


Asn Gly Ile Gly Ile Gly Glu Phe Lys Asp Ser Leu Ser Ile Asn Ala 
        35                  40                  45              


Thr Asn Ile Lys His Phe Lys Asn Cys Thr Ser Ile Ser Gly Asp Leu 
    50                  55                  60                  


His Ile Leu Pro Val Ala Phe Arg Gly Asp Ser Phe Thr His Thr Pro 
65                  70                  75                  80  


Pro Leu Asp Pro Gln Glu Leu Asp Ile Leu Lys Thr Val Lys Glu Ile 
                85                  90                  95      


Thr Gly Phe Leu Leu Ile Gln Ala Trp Pro Glu Asn Arg Thr Asp Leu 
            100                 105                 110         


His Ala Phe Glu Asn Leu Glu Ile Ile Arg Gly Arg Thr Lys Gln His 
        115                 120                 125             


Gly Gln Phe Ser Leu Ala Val Val Ser Leu Asn Ile Thr Ser Leu Gly 
    130                 135                 140                 


Leu Arg Ser Leu Lys Glu Ile Ser Asp Gly Asp Val Ile Ile Ser Gly 
145                 150                 155                 160 


Asn Lys Asn Leu Cys Tyr Ala Asn Thr Ile Asn Trp Lys Lys Leu Phe 
                165                 170                 175     


Gly Thr Ser Gly Gln Lys Thr Lys Ile Ile Ser Asn Arg Gly Glu Asn 
            180                 185                 190         


Ser Cys Lys Ala Thr Gly Gln Val Cys His Ala Leu Cys Ser Pro Glu 
        195                 200                 205             


Gly Cys Trp Gly Pro Glu Pro Arg Asp Cys Val Ser Cys Arg Asn Val 
    210                 215                 220                 


Ser Arg Gly Arg Glu Cys Val Asp Lys Cys Asn Leu Leu Glu Gly Glu 
225                 230                 235                 240 


Pro Arg Glu Phe Val Glu Asn Ser Glu Cys Ile Gln Cys His Pro Glu 
                245                 250                 255     


Cys Leu Pro Gln Ala Met Asn Ile Thr Cys Thr Gly Arg Gly Pro Asp 
            260                 265                 270         


Asn Cys Ile Gln Cys Ala His Tyr Ile Asp Gly Pro His Cys Val Lys 
        275                 280                 285             


Thr Cys Pro Ala Gly Val Met Gly Glu Asn Asn Thr Leu Val Trp Lys 
    290                 295                 300                 


Tyr Ala Asp Ala Gly His Val Cys His Leu Cys His Pro Asn Cys Thr 
305                 310                 315                 320 


Tyr Gly Cys Thr Gly Pro Gly Leu Glu Gly Cys Pro Thr Asn Gly Pro 
                325                 330                 335     


Lys Ile Pro Ser Ile Ala Thr Gly Met Val Gly Ala Leu Leu Leu Leu 
            340                 345                 350         


Leu Val Val Ala Leu Gly Ile Gly Leu Phe Met 
        355                 360             


<210>  18
<211>  1038
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  nucleotide sequence encoding modified EGFRt (with NGFR 
       cytoplasmic domain)

<400>  18
agaaaagtgt gcaacggcat cggcatcgga gagttcaagg acagcctgag catcaacgcc       60

accaacatca agcacttcaa gaactgcacc agcatcagcg gcgacctgca cattctgcct      120

gtggccttta gaggcgacag cttcacccac acacctccac tggatcccca agagctggac      180

atcctgaaaa ccgtgaaaga gatcaccgga tttctgttga tccaggcttg gcccgagaac      240

cggacagatc tgcacgcctt cgagaacctg gaaatcatca gaggccggac caagcagcac      300

ggccagtttt ctctggctgt ggtgtccctg aacatcacca gcctgggcct gagaagcctg      360

aaagaaatca gcgacggcga cgtgatcatc tccggcaaca agaacctgtg ctacgccaac      420

accatcaact ggaagaagct gttcggcacc agcggccaga aaacaaagat catcagcaac      480

cggggcgaga acagctgcaa ggctacaggc caagtgtgcc acgctctgtg tagccctgaa      540

ggctgttggg gacccgagcc tagagattgc gtgtcctgca gaaacgtgtc ccggggcaga      600

gaatgcgtgg acaagtgcaa tctgctggaa ggcgagcccc gcgagttcgt ggaaaacagc      660

gagtgcatcc agtgtcaccc cgagtgtctg ccccaggcca tgaacattac ctgtaccggc      720

agaggccccg acaactgtat tcagtgcgcc cactacatcg acggccctca ctgcgtgaaa      780

acatgtcctg ctggcgtgat gggagagaac aacaccctcg tgtggaagta tgccgacgcc      840

ggacatgtgt gccacctgtg tcaccctaat tgcacctacg gctgtacagg ccctggcctg      900

gaaggctgtc caacaaacgg acctaagatc ccctctatcg ccaccggcat ggttggagcc      960

ctgctgcttc tgctggtggt ggcccttgga atcggcctgt ttatgaagcg gtggaaccgg     1020

ggcatcctgg ctagctga                                                   1038


<210>  19
<211>  345
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  modified EGFRt (with NGFR cytoplasmic domain)

<400>  19

Arg Lys Val Cys Asn Gly Ile Gly Ile Gly Glu Phe Lys Asp Ser Leu 
1               5                   10                  15      


Ser Ile Asn Ala Thr Asn Ile Lys His Phe Lys Asn Cys Thr Ser Ile 
            20                  25                  30          


Ser Gly Asp Leu His Ile Leu Pro Val Ala Phe Arg Gly Asp Ser Phe 
        35                  40                  45              


Thr His Thr Pro Pro Leu Asp Pro Gln Glu Leu Asp Ile Leu Lys Thr 
    50                  55                  60                  


Val Lys Glu Ile Thr Gly Phe Leu Leu Ile Gln Ala Trp Pro Glu Asn 
65                  70                  75                  80  


Arg Thr Asp Leu His Ala Phe Glu Asn Leu Glu Ile Ile Arg Gly Arg 
                85                  90                  95      


Thr Lys Gln His Gly Gln Phe Ser Leu Ala Val Val Ser Leu Asn Ile 
            100                 105                 110         


Thr Ser Leu Gly Leu Arg Ser Leu Lys Glu Ile Ser Asp Gly Asp Val 
        115                 120                 125             


Ile Ile Ser Gly Asn Lys Asn Leu Cys Tyr Ala Asn Thr Ile Asn Trp 
    130                 135                 140                 


Lys Lys Leu Phe Gly Thr Ser Gly Gln Lys Thr Lys Ile Ile Ser Asn 
145                 150                 155                 160 


Arg Gly Glu Asn Ser Cys Lys Ala Thr Gly Gln Val Cys His Ala Leu 
                165                 170                 175     


Cys Ser Pro Glu Gly Cys Trp Gly Pro Glu Pro Arg Asp Cys Val Ser 
            180                 185                 190         


Cys Arg Asn Val Ser Arg Gly Arg Glu Cys Val Asp Lys Cys Asn Leu 
        195                 200                 205             


Leu Glu Gly Glu Pro Arg Glu Phe Val Glu Asn Ser Glu Cys Ile Gln 
    210                 215                 220                 


Cys His Pro Glu Cys Leu Pro Gln Ala Met Asn Ile Thr Cys Thr Gly 
225                 230                 235                 240 


Arg Gly Pro Asp Asn Cys Ile Gln Cys Ala His Tyr Ile Asp Gly Pro 
                245                 250                 255     


His Cys Val Lys Thr Cys Pro Ala Gly Val Met Gly Glu Asn Asn Thr 
            260                 265                 270         


Leu Val Trp Lys Tyr Ala Asp Ala Gly His Val Cys His Leu Cys His 
        275                 280                 285             


Pro Asn Cys Thr Tyr Gly Cys Thr Gly Pro Gly Leu Glu Gly Cys Pro 
    290                 295                 300                 


Thr Asn Gly Pro Lys Ile Pro Ser Ile Ala Thr Gly Met Val Gly Ala 
305                 310                 315                 320 


Leu Leu Leu Leu Leu Val Val Ala Leu Gly Ile Gly Leu Phe Met Lys 
                325                 330                 335     


Arg Trp Asn Arg Gly Ile Leu Ala Ser 
            340                 345 


<210>  20
<211>  1038
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  nucleotide sequence encoding modified EGFRt (with EGFR 
       cytoplasmic domain)

<400>  20
agaaaagtgt gcaacggcat cggcatcgga gagttcaagg acagcctgag catcaacgcc       60

accaacatca agcacttcaa gaactgcacc agcatcagcg gcgacctgca cattctgcct      120

gtggccttta gaggcgacag cttcacccac acacctccac tggatcccca agagctggac      180

atcctgaaaa ccgtgaaaga gatcaccgga tttctgttga tccaggcttg gcccgagaac      240

cggacagatc tgcacgcctt cgagaacctg gaaatcatca gaggccggac caagcagcac      300

ggccagtttt ctctggctgt ggtgtccctg aacatcacca gcctgggcct gagaagcctg      360

aaagaaatca gcgacggcga cgtgatcatc tccggcaaca agaacctgtg ctacgccaac      420

accatcaact ggaagaagct gttcggcacc agcggccaga aaacaaagat catcagcaac      480

cggggcgaga acagctgcaa ggctacaggc caagtgtgcc acgctctgtg tagccctgaa      540

ggctgttggg gacccgagcc tagagattgc gtgtcctgca gaaacgtgtc ccggggcaga      600

gaatgcgtgg acaagtgcaa tctgctggaa ggcgagcccc gcgagttcgt ggaaaacagc      660

gagtgcatcc agtgtcaccc cgagtgtctg ccccaggcca tgaacattac ctgtaccggc      720

agaggccccg acaactgtat tcagtgcgcc cactacatcg acggccctca ctgcgtgaaa      780

acatgtcctg ctggcgtgat gggagagaac aacaccctcg tgtggaagta tgccgacgcc      840

ggacatgtgt gccacctgtg tcaccctaat tgcacctacg gctgtacagg ccctggcctg      900

gaaggctgtc caacaaacgg acctaagatc ccctctatcg ccaccggcat ggttggagcc      960

ctgctgcttc tgctggtggt ggcccttgga atcggcctgt ttatgcggcg gagacacatc     1020

gtgcggaagg ctagctga                                                   1038


<210>  21
<211>  345
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  modified EGFRt (with EGFR cytoplasmic domain)

<400>  21

Arg Lys Val Cys Asn Gly Ile Gly Ile Gly Glu Phe Lys Asp Ser Leu 
1               5                   10                  15      


Ser Ile Asn Ala Thr Asn Ile Lys His Phe Lys Asn Cys Thr Ser Ile 
            20                  25                  30          


Ser Gly Asp Leu His Ile Leu Pro Val Ala Phe Arg Gly Asp Ser Phe 
        35                  40                  45              


Thr His Thr Pro Pro Leu Asp Pro Gln Glu Leu Asp Ile Leu Lys Thr 
    50                  55                  60                  


Val Lys Glu Ile Thr Gly Phe Leu Leu Ile Gln Ala Trp Pro Glu Asn 
65                  70                  75                  80  


Arg Thr Asp Leu His Ala Phe Glu Asn Leu Glu Ile Ile Arg Gly Arg 
                85                  90                  95      


Thr Lys Gln His Gly Gln Phe Ser Leu Ala Val Val Ser Leu Asn Ile 
            100                 105                 110         


Thr Ser Leu Gly Leu Arg Ser Leu Lys Glu Ile Ser Asp Gly Asp Val 
        115                 120                 125             


Ile Ile Ser Gly Asn Lys Asn Leu Cys Tyr Ala Asn Thr Ile Asn Trp 
    130                 135                 140                 


Lys Lys Leu Phe Gly Thr Ser Gly Gln Lys Thr Lys Ile Ile Ser Asn 
145                 150                 155                 160 


Arg Gly Glu Asn Ser Cys Lys Ala Thr Gly Gln Val Cys His Ala Leu 
                165                 170                 175     


Cys Ser Pro Glu Gly Cys Trp Gly Pro Glu Pro Arg Asp Cys Val Ser 
            180                 185                 190         


Cys Arg Asn Val Ser Arg Gly Arg Glu Cys Val Asp Lys Cys Asn Leu 
        195                 200                 205             


Leu Glu Gly Glu Pro Arg Glu Phe Val Glu Asn Ser Glu Cys Ile Gln 
    210                 215                 220                 


Cys His Pro Glu Cys Leu Pro Gln Ala Met Asn Ile Thr Cys Thr Gly 
225                 230                 235                 240 


Arg Gly Pro Asp Asn Cys Ile Gln Cys Ala His Tyr Ile Asp Gly Pro 
                245                 250                 255     


His Cys Val Lys Thr Cys Pro Ala Gly Val Met Gly Glu Asn Asn Thr 
            260                 265                 270         


Leu Val Trp Lys Tyr Ala Asp Ala Gly His Val Cys His Leu Cys His 
        275                 280                 285             


Pro Asn Cys Thr Tyr Gly Cys Thr Gly Pro Gly Leu Glu Gly Cys Pro 
    290                 295                 300                 


Thr Asn Gly Pro Lys Ile Pro Ser Ile Ala Thr Gly Met Val Gly Ala 
305                 310                 315                 320 


Leu Leu Leu Leu Leu Val Val Ala Leu Gly Ile Gly Leu Phe Met Arg 
                325                 330                 335     


Arg Arg His Ile Val Arg Lys Ala Ser 
            340                 345 


<210>  22
<211>  1122
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  nucleotide sequence encoding modified EGFRt (with NGFR signal 
       peptide and NGFR cytoplasmic domain)

<400>  22
atgggagctg gtgctaccgg cagagctatg gatggaccta gactgctgct cctgctgctg       60

ctcggagttt ctcttggcgg agccagaaaa gtgtgcaacg gcatcggcat cggagagttc      120

aaggacagcc tgagcatcaa cgccaccaac atcaagcact tcaagaactg caccagcatc      180

agcggcgacc tgcacattct gcctgtggcc tttagaggcg acagcttcac ccacacacct      240

ccactggatc cccaagagct ggacatcctg aaaaccgtga aagagatcac cggatttctg      300

ttgatccagg cttggcccga gaaccggaca gatctgcacg ccttcgagaa cctggaaatc      360

atcagaggcc ggaccaagca gcacggccag ttttctctgg ctgtggtgtc cctgaacatc      420

accagcctgg gcctgagaag cctgaaagaa atcagcgacg gcgacgtgat catctccggc      480

aacaagaacc tgtgctacgc caacaccatc aactggaaga agctgttcgg caccagcggc      540

cagaaaacaa agatcatcag caaccggggc gagaacagct gcaaggctac aggccaagtg      600

tgccacgctc tgtgtagccc tgaaggctgt tggggacccg agcctagaga ttgcgtgtcc      660

tgcagaaacg tgtcccgggg cagagaatgc gtggacaagt gcaatctgct ggaaggcgag      720

ccccgcgagt tcgtggaaaa cagcgagtgc atccagtgtc accccgagtg tctgccccag      780

gccatgaaca ttacctgtac cggcagaggc cccgacaact gtattcagtg cgcccactac      840

atcgacggcc ctcactgcgt gaaaacatgt cctgctggcg tgatgggaga gaacaacacc      900

ctcgtgtgga agtatgccga cgccggacat gtgtgccacc tgtgtcaccc taattgcacc      960

tacggctgta caggccctgg cctggaaggc tgtccaacaa acggacctaa gatcccctct     1020

atcgccaccg gcatggttgg agccctgctg cttctgctgg tggtggccct tggaatcggc     1080

ctgtttatga agcggtggaa ccggggcatc ctggctagct ga                        1122


<210>  23
<211>  373
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  modified EGFRt (with NGFR signal peptide and NGFR cytoplasmic 
       domain)

<400>  23

Met Gly Ala Gly Ala Thr Gly Arg Ala Met Asp Gly Pro Arg Leu Leu 
1               5                   10                  15      


Leu Leu Leu Leu Leu Gly Val Ser Leu Gly Gly Ala Arg Lys Val Cys 
            20                  25                  30          


Asn Gly Ile Gly Ile Gly Glu Phe Lys Asp Ser Leu Ser Ile Asn Ala 
        35                  40                  45              


Thr Asn Ile Lys His Phe Lys Asn Cys Thr Ser Ile Ser Gly Asp Leu 
    50                  55                  60                  


His Ile Leu Pro Val Ala Phe Arg Gly Asp Ser Phe Thr His Thr Pro 
65                  70                  75                  80  


Pro Leu Asp Pro Gln Glu Leu Asp Ile Leu Lys Thr Val Lys Glu Ile 
                85                  90                  95      


Thr Gly Phe Leu Leu Ile Gln Ala Trp Pro Glu Asn Arg Thr Asp Leu 
            100                 105                 110         


His Ala Phe Glu Asn Leu Glu Ile Ile Arg Gly Arg Thr Lys Gln His 
        115                 120                 125             


Gly Gln Phe Ser Leu Ala Val Val Ser Leu Asn Ile Thr Ser Leu Gly 
    130                 135                 140                 


Leu Arg Ser Leu Lys Glu Ile Ser Asp Gly Asp Val Ile Ile Ser Gly 
145                 150                 155                 160 


Asn Lys Asn Leu Cys Tyr Ala Asn Thr Ile Asn Trp Lys Lys Leu Phe 
                165                 170                 175     


Gly Thr Ser Gly Gln Lys Thr Lys Ile Ile Ser Asn Arg Gly Glu Asn 
            180                 185                 190         


Ser Cys Lys Ala Thr Gly Gln Val Cys His Ala Leu Cys Ser Pro Glu 
        195                 200                 205             


Gly Cys Trp Gly Pro Glu Pro Arg Asp Cys Val Ser Cys Arg Asn Val 
    210                 215                 220                 


Ser Arg Gly Arg Glu Cys Val Asp Lys Cys Asn Leu Leu Glu Gly Glu 
225                 230                 235                 240 


Pro Arg Glu Phe Val Glu Asn Ser Glu Cys Ile Gln Cys His Pro Glu 
                245                 250                 255     


Cys Leu Pro Gln Ala Met Asn Ile Thr Cys Thr Gly Arg Gly Pro Asp 
            260                 265                 270         


Asn Cys Ile Gln Cys Ala His Tyr Ile Asp Gly Pro His Cys Val Lys 
        275                 280                 285             


Thr Cys Pro Ala Gly Val Met Gly Glu Asn Asn Thr Leu Val Trp Lys 
    290                 295                 300                 


Tyr Ala Asp Ala Gly His Val Cys His Leu Cys His Pro Asn Cys Thr 
305                 310                 315                 320 


Tyr Gly Cys Thr Gly Pro Gly Leu Glu Gly Cys Pro Thr Asn Gly Pro 
                325                 330                 335     


Lys Ile Pro Ser Ile Ala Thr Gly Met Val Gly Ala Leu Leu Leu Leu 
            340                 345                 350         


Leu Val Val Ala Leu Gly Ile Gly Leu Phe Met Lys Arg Trp Asn Arg 
        355                 360                 365             


Gly Ile Leu Ala Ser 
    370             


<210>  24
<211>  1122
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  nucleotide sequence encoding modified EGFRt (with NGFR signal 
       peptide and EGFR cytoplasmic domain)

<400>  24
atgggagctg gtgctaccgg cagagctatg gatggaccta gactgctgct cctgctgctg       60

ctcggagttt ctcttggcgg agccagaaaa gtgtgcaacg gcatcggcat cggagagttc      120

aaggacagcc tgagcatcaa cgccaccaac atcaagcact tcaagaactg caccagcatc      180

agcggcgacc tgcacattct gcctgtggcc tttagaggcg acagcttcac ccacacacct      240

ccactggatc cccaagagct ggacatcctg aaaaccgtga aagagatcac cggatttctg      300

ttgatccagg cttggcccga gaaccggaca gatctgcacg ccttcgagaa cctggaaatc      360

atcagaggcc ggaccaagca gcacggccag ttttctctgg ctgtggtgtc cctgaacatc      420

accagcctgg gcctgagaag cctgaaagaa atcagcgacg gcgacgtgat catctccggc      480

aacaagaacc tgtgctacgc caacaccatc aactggaaga agctgttcgg caccagcggc      540

cagaaaacaa agatcatcag caaccggggc gagaacagct gcaaggctac aggccaagtg      600

tgccacgctc tgtgtagccc tgaaggctgt tggggacccg agcctagaga ttgcgtgtcc      660

tgcagaaacg tgtcccgggg cagagaatgc gtggacaagt gcaatctgct ggaaggcgag      720

ccccgcgagt tcgtggaaaa cagcgagtgc atccagtgtc accccgagtg tctgccccag      780

gccatgaaca ttacctgtac cggcagaggc cccgacaact gtattcagtg cgcccactac      840

atcgacggcc ctcactgcgt gaaaacatgt cctgctggcg tgatgggaga gaacaacacc      900

ctcgtgtgga agtatgccga cgccggacat gtgtgccacc tgtgtcaccc taattgcacc      960

tacggctgta caggccctgg cctggaaggc tgtccaacaa acggacctaa gatcccctct     1020

atcgccaccg gcatggttgg agccctgctg cttctgctgg tggtggccct tggaatcggc     1080

ctgtttatgc ggcggagaca catcgtgcgg aaggctagct ga                        1122


<210>  25
<211>  373
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  modified EGFRt (with NGFR signal peptide and EGFR cytoplasmic 
       domain)

<400>  25

Met Gly Ala Gly Ala Thr Gly Arg Ala Met Asp Gly Pro Arg Leu Leu 
1               5                   10                  15      


Leu Leu Leu Leu Leu Gly Val Ser Leu Gly Gly Ala Arg Lys Val Cys 
            20                  25                  30          


Asn Gly Ile Gly Ile Gly Glu Phe Lys Asp Ser Leu Ser Ile Asn Ala 
        35                  40                  45              


Thr Asn Ile Lys His Phe Lys Asn Cys Thr Ser Ile Ser Gly Asp Leu 
    50                  55                  60                  


His Ile Leu Pro Val Ala Phe Arg Gly Asp Ser Phe Thr His Thr Pro 
65                  70                  75                  80  


Pro Leu Asp Pro Gln Glu Leu Asp Ile Leu Lys Thr Val Lys Glu Ile 
                85                  90                  95      


Thr Gly Phe Leu Leu Ile Gln Ala Trp Pro Glu Asn Arg Thr Asp Leu 
            100                 105                 110         


His Ala Phe Glu Asn Leu Glu Ile Ile Arg Gly Arg Thr Lys Gln His 
        115                 120                 125             


Gly Gln Phe Ser Leu Ala Val Val Ser Leu Asn Ile Thr Ser Leu Gly 
    130                 135                 140                 


Leu Arg Ser Leu Lys Glu Ile Ser Asp Gly Asp Val Ile Ile Ser Gly 
145                 150                 155                 160 


Asn Lys Asn Leu Cys Tyr Ala Asn Thr Ile Asn Trp Lys Lys Leu Phe 
                165                 170                 175     


Gly Thr Ser Gly Gln Lys Thr Lys Ile Ile Ser Asn Arg Gly Glu Asn 
            180                 185                 190         


Ser Cys Lys Ala Thr Gly Gln Val Cys His Ala Leu Cys Ser Pro Glu 
        195                 200                 205             


Gly Cys Trp Gly Pro Glu Pro Arg Asp Cys Val Ser Cys Arg Asn Val 
    210                 215                 220                 


Ser Arg Gly Arg Glu Cys Val Asp Lys Cys Asn Leu Leu Glu Gly Glu 
225                 230                 235                 240 


Pro Arg Glu Phe Val Glu Asn Ser Glu Cys Ile Gln Cys His Pro Glu 
                245                 250                 255     


Cys Leu Pro Gln Ala Met Asn Ile Thr Cys Thr Gly Arg Gly Pro Asp 
            260                 265                 270         


Asn Cys Ile Gln Cys Ala His Tyr Ile Asp Gly Pro His Cys Val Lys 
        275                 280                 285             


Thr Cys Pro Ala Gly Val Met Gly Glu Asn Asn Thr Leu Val Trp Lys 
    290                 295                 300                 


Tyr Ala Asp Ala Gly His Val Cys His Leu Cys His Pro Asn Cys Thr 
305                 310                 315                 320 


Tyr Gly Cys Thr Gly Pro Gly Leu Glu Gly Cys Pro Thr Asn Gly Pro 
                325                 330                 335     


Lys Ile Pro Ser Ile Ala Thr Gly Met Val Gly Ala Leu Leu Leu Leu 
            340                 345                 350         


Leu Val Val Ala Leu Gly Ile Gly Leu Phe Met Arg Arg Arg His Ile 
        355                 360                 365             


Val Arg Lys Ala Ser 
    370             


<210>  26
<211>  1074
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  comparative nucleotide sequence encoding an EGFRt

<400>  26
atgctgctgc tggtcacctc tctgctgctg tgcgaactgc cccatcctgc ctttctgctg       60

atccccagaa aagtgtgcaa cggcatcggc atcggagagt tcaaggacag cctgagcatc      120

aacgccacca acatcaagca cttcaagaac tgcaccagca tcagcggcga cctgcacatt      180

ctgcctgtgg cctttagagg cgacagcttc acccacacac ctccactgga tccccaagag      240

ctggacatcc tgaaaaccgt gaaagagatc accggatttc tgttgatcca ggcttggccc      300

gagaaccgga cagatctgca cgccttcgag aacctggaaa tcatcagagg ccggaccaag      360

cagcacggcc agttttctct ggctgtggtg tccctgaaca tcaccagcct gggcctgaga      420

agcctgaaag aaatcagcga cggcgacgtg atcatctccg gcaacaagaa cctgtgctac      480

gccaacacca tcaactggaa gaagctgttc ggcaccagcg gccagaaaac aaagatcatc      540

agcaaccggg gcgagaacag ctgcaaggct acaggccaag tgtgccacgc tctgtgtagc      600

cctgaaggct gttggggacc cgagcctaga gattgcgtgt cctgcagaaa cgtgtcccgg      660

ggcagagaat gcgtggacaa gtgcaatctg ctggaaggcg agccccgcga gttcgtggaa      720

aacagcgagt gcatccagtg tcaccccgag tgtctgcccc aggccatgaa cattacctgt      780

accggcagag gccccgacaa ctgtattcag tgcgcccact acatcgacgg ccctcactgc      840

gtgaaaacat gtcctgctgg cgtgatggga gagaacaaca ccctcgtgtg gaagtatgcc      900

gacgccggac atgtgtgcca cctgtgtcac cctaattgca cctacggctg tacaggccct      960

ggcctggaag gctgtccaac aaacggacct aagatcccct ctatcgccac cggcatggtt     1020

ggagccctgc tgcttctgct ggtggtggcc cttggaatcg gcctgtttat gtag           1074


<210>  27
<211>  357
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  comparative EGFRt sequence

<400>  27

Met Leu Leu Leu Val Thr Ser Leu Leu Leu Cys Glu Leu Pro His Pro 
1               5                   10                  15      


Ala Phe Leu Leu Ile Pro Arg Lys Val Cys Asn Gly Ile Gly Ile Gly 
            20                  25                  30          


Glu Phe Lys Asp Ser Leu Ser Ile Asn Ala Thr Asn Ile Lys His Phe 
        35                  40                  45              


Lys Asn Cys Thr Ser Ile Ser Gly Asp Leu His Ile Leu Pro Val Ala 
    50                  55                  60                  


Phe Arg Gly Asp Ser Phe Thr His Thr Pro Pro Leu Asp Pro Gln Glu 
65                  70                  75                  80  


Leu Asp Ile Leu Lys Thr Val Lys Glu Ile Thr Gly Phe Leu Leu Ile 
                85                  90                  95      


Gln Ala Trp Pro Glu Asn Arg Thr Asp Leu His Ala Phe Glu Asn Leu 
            100                 105                 110         


Glu Ile Ile Arg Gly Arg Thr Lys Gln His Gly Gln Phe Ser Leu Ala 
        115                 120                 125             


Val Val Ser Leu Asn Ile Thr Ser Leu Gly Leu Arg Ser Leu Lys Glu 
    130                 135                 140                 


Ile Ser Asp Gly Asp Val Ile Ile Ser Gly Asn Lys Asn Leu Cys Tyr 
145                 150                 155                 160 


Ala Asn Thr Ile Asn Trp Lys Lys Leu Phe Gly Thr Ser Gly Gln Lys 
                165                 170                 175     


Thr Lys Ile Ile Ser Asn Arg Gly Glu Asn Ser Cys Lys Ala Thr Gly 
            180                 185                 190         


Gln Val Cys His Ala Leu Cys Ser Pro Glu Gly Cys Trp Gly Pro Glu 
        195                 200                 205             


Pro Arg Asp Cys Val Ser Cys Arg Asn Val Ser Arg Gly Arg Glu Cys 
    210                 215                 220                 


Val Asp Lys Cys Asn Leu Leu Glu Gly Glu Pro Arg Glu Phe Val Glu 
225                 230                 235                 240 


Asn Ser Glu Cys Ile Gln Cys His Pro Glu Cys Leu Pro Gln Ala Met 
                245                 250                 255     


Asn Ile Thr Cys Thr Gly Arg Gly Pro Asp Asn Cys Ile Gln Cys Ala 
            260                 265                 270         


His Tyr Ile Asp Gly Pro His Cys Val Lys Thr Cys Pro Ala Gly Val 
        275                 280                 285             


Met Gly Glu Asn Asn Thr Leu Val Trp Lys Tyr Ala Asp Ala Gly His 
    290                 295                 300                 


Val Cys His Leu Cys His Pro Asn Cys Thr Tyr Gly Cys Thr Gly Pro 
305                 310                 315                 320 


Gly Leu Glu Gly Cys Pro Thr Asn Gly Pro Lys Ile Pro Ser Ile Ala 
                325                 330                 335     


Thr Gly Met Val Gly Ala Leu Leu Leu Leu Leu Val Val Ala Leu Gly 
            340                 345                 350         


Ile Gly Leu Phe Met 
        355         


<210>  28
<211>  1146
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  nucleotide sequence encoding modified EGFRt (with GMS SFR alpha 
       signal peptide; and NGFR transmembrane and cytoplasmic domain)

<400>  28
atgctgctgc tggtcacctc tctgctgctg tgcgaactgc cccatcctgc ctttctgctg       60

atccccagaa aagtgtgcaa cggcatcggc atcggagagt tcaaggacag cctgagcatc      120

aacgccacca acatcaagca cttcaagaac tgcaccagca tcagcggcga cctgcacatt      180

ctgcctgtgg cctttagagg cgacagcttc acccacacac ctccactgga tccccaagag      240

ctggacatcc tgaaaaccgt gaaagagatc accggatttc tgttgatcca ggcttggccc      300

gagaaccgga cagatctgca cgccttcgag aacctggaaa tcatcagagg ccggaccaag      360

cagcacggcc agttttctct ggctgtggtg tccctgaaca tcaccagcct gggcctgaga      420

agcctgaaag aaatcagcga cggcgacgtg atcatctccg gcaacaagaa cctgtgctac      480

gccaacacca tcaactggaa gaagctgttc ggcaccagcg gccagaaaac aaagatcatc      540

agcaaccggg gcgagaacag ctgcaaggct acaggccaag tgtgccacgc tctgtgtagc      600

cctgaaggct gttggggacc cgagcctaga gattgcgtgt cctgcagaaa cgtgtcccgg      660

ggcagagaat gcgtggacaa gtgcaatctg ctggaaggcg agccccgcga gttcgtggaa      720

aacagcgagt gcatccagtg tcaccccgag tgtctgcccc aggccatgaa cattacctgt      780

accggcagag gccccgacaa ctgtattcag tgcgcccact acatcgacgg ccctcactgc      840

gtgaaaacat gtcctgctgg cgtgatggga gagaacaaca ccctcgtgtg gaagtatgcc      900

gacgccggac atgtgtgcca cctgtgtcac cctaattgca cctacggctg tacaggccct      960

ggcctggaag gctgtccaac aaacggacct aagatcccct ctggcggcgg aggatctggc     1020

ggaggtggaa gcggaggcgg tggatctctg atccccgtgt actgtagcat cctggccgcc     1080

gtggttgtgg gactcgtggc ctatatcgcc ttcaagcggt ggaaccgggg catcctggct     1140

agctga                                                                1146


<210>  29
<211>  381
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  modified EGFRt (with GMS SFR alpha signal peptide; and NGFR 
       transmembrane and cytoplasmic domain)

<400>  29

Met Leu Leu Leu Val Thr Ser Leu Leu Leu Cys Glu Leu Pro His Pro 
1               5                   10                  15      


Ala Phe Leu Leu Ile Pro Arg Lys Val Cys Asn Gly Ile Gly Ile Gly 
            20                  25                  30          


Glu Phe Lys Asp Ser Leu Ser Ile Asn Ala Thr Asn Ile Lys His Phe 
        35                  40                  45              


Lys Asn Cys Thr Ser Ile Ser Gly Asp Leu His Ile Leu Pro Val Ala 
    50                  55                  60                  


Phe Arg Gly Asp Ser Phe Thr His Thr Pro Pro Leu Asp Pro Gln Glu 
65                  70                  75                  80  


Leu Asp Ile Leu Lys Thr Val Lys Glu Ile Thr Gly Phe Leu Leu Ile 
                85                  90                  95      


Gln Ala Trp Pro Glu Asn Arg Thr Asp Leu His Ala Phe Glu Asn Leu 
            100                 105                 110         


Glu Ile Ile Arg Gly Arg Thr Lys Gln His Gly Gln Phe Ser Leu Ala 
        115                 120                 125             


Val Val Ser Leu Asn Ile Thr Ser Leu Gly Leu Arg Ser Leu Lys Glu 
    130                 135                 140                 


Ile Ser Asp Gly Asp Val Ile Ile Ser Gly Asn Lys Asn Leu Cys Tyr 
145                 150                 155                 160 


Ala Asn Thr Ile Asn Trp Lys Lys Leu Phe Gly Thr Ser Gly Gln Lys 
                165                 170                 175     


Thr Lys Ile Ile Ser Asn Arg Gly Glu Asn Ser Cys Lys Ala Thr Gly 
            180                 185                 190         


Gln Val Cys His Ala Leu Cys Ser Pro Glu Gly Cys Trp Gly Pro Glu 
        195                 200                 205             


Pro Arg Asp Cys Val Ser Cys Arg Asn Val Ser Arg Gly Arg Glu Cys 
    210                 215                 220                 


Val Asp Lys Cys Asn Leu Leu Glu Gly Glu Pro Arg Glu Phe Val Glu 
225                 230                 235                 240 


Asn Ser Glu Cys Ile Gln Cys His Pro Glu Cys Leu Pro Gln Ala Met 
                245                 250                 255     


Asn Ile Thr Cys Thr Gly Arg Gly Pro Asp Asn Cys Ile Gln Cys Ala 
            260                 265                 270         


His Tyr Ile Asp Gly Pro His Cys Val Lys Thr Cys Pro Ala Gly Val 
        275                 280                 285             


Met Gly Glu Asn Asn Thr Leu Val Trp Lys Tyr Ala Asp Ala Gly His 
    290                 295                 300                 


Val Cys His Leu Cys His Pro Asn Cys Thr Tyr Gly Cys Thr Gly Pro 
305                 310                 315                 320 


Gly Leu Glu Gly Cys Pro Thr Asn Gly Pro Lys Ile Pro Ser Gly Gly 
                325                 330                 335     


Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Leu Ile Pro 
            340                 345                 350         


Val Tyr Cys Ser Ile Leu Ala Ala Val Val Val Gly Leu Val Ala Tyr 
        355                 360                 365             


Ile Ala Phe Lys Arg Trp Asn Arg Gly Ile Leu Ala Ser 
    370                 375                 380     


<210>  30
<211>  1194
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  nucleotide sequence encoding modified EGFRt (with NGFR signal 
       peptide, NGFR cytoplasmic domain and recycling signal)

<400>  30
atgggagctg gtgctaccgg cagagctatg gatggaccta gactgctgct cctgctgctg       60

ctcggagttt ctcttggcgg agccagaaaa gtgtgcaacg gcatcggcat cggagagttc      120

aaggacagcc tgagcatcaa cgccaccaac atcaagcact tcaagaactg caccagcatc      180

agcggcgacc tgcacattct gcctgtggcc tttagaggcg acagcttcac ccacacacct      240

ccactggatc cccaagagct ggacatcctg aaaaccgtga aagagatcac cggatttctg      300

ttgatccagg cttggcccga gaaccggaca gatctgcacg ccttcgagaa cctggaaatc      360

atcagaggcc ggaccaagca gcacggccag ttttctctgg ctgtggtgtc cctgaacatc      420

accagcctgg gcctgagaag cctgaaagaa atcagcgacg gcgacgtgat catctccggc      480

aacaagaacc tgtgctacgc caacaccatc aactggaaga agctgttcgg caccagcggc      540

cagaaaacaa agatcatcag caaccggggc gagaacagct gcaaggctac aggccaagtg      600

tgccacgctc tgtgtagccc tgaaggctgt tggggacccg agcctagaga ttgcgtgtcc      660

tgcagaaacg tgtcccgggg cagagaatgc gtggacaagt gcaatctgct ggaaggcgag      720

ccccgcgagt tcgtggaaaa cagcgagtgc atccagtgtc accccgagtg tctgccccag      780

gccatgaaca ttacctgtac cggcagaggc cccgacaact gtattcagtg cgcccactac      840

atcgacggcc ctcactgcgt gaaaacatgt cctgctggcg tgatgggaga gaacaacacc      900

ctcgtgtgga agtatgccga cgccggacat gtgtgccacc tgtgtcaccc taattgcacc      960

tacggctgta caggccctgg cctggaaggc tgtccaacaa acggacctaa gatcccctct     1020

atcgccaccg gcatggttgg agccctgctg cttctgctgg tggtggccct tggaatcggc     1080

ctgtttatga agcggtggaa ccggggcatc ctggctagct atcagcctct gagccagatc     1140

aagcggctgc tgagcgactc cttcctgttc gacaaccccg tgtacgctag ctga           1194


<210>  31
<211>  397
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  modified EGFRt (with NGFR signal peptide, NGFR cytoplasmic domain
       and recycling signal)

<400>  31

Met Gly Ala Gly Ala Thr Gly Arg Ala Met Asp Gly Pro Arg Leu Leu 
1               5                   10                  15      


Leu Leu Leu Leu Leu Gly Val Ser Leu Gly Gly Ala Arg Lys Val Cys 
            20                  25                  30          


Asn Gly Ile Gly Ile Gly Glu Phe Lys Asp Ser Leu Ser Ile Asn Ala 
        35                  40                  45              


Thr Asn Ile Lys His Phe Lys Asn Cys Thr Ser Ile Ser Gly Asp Leu 
    50                  55                  60                  


His Ile Leu Pro Val Ala Phe Arg Gly Asp Ser Phe Thr His Thr Pro 
65                  70                  75                  80  


Pro Leu Asp Pro Gln Glu Leu Asp Ile Leu Lys Thr Val Lys Glu Ile 
                85                  90                  95      


Thr Gly Phe Leu Leu Ile Gln Ala Trp Pro Glu Asn Arg Thr Asp Leu 
            100                 105                 110         


His Ala Phe Glu Asn Leu Glu Ile Ile Arg Gly Arg Thr Lys Gln His 
        115                 120                 125             


Gly Gln Phe Ser Leu Ala Val Val Ser Leu Asn Ile Thr Ser Leu Gly 
    130                 135                 140                 


Leu Arg Ser Leu Lys Glu Ile Ser Asp Gly Asp Val Ile Ile Ser Gly 
145                 150                 155                 160 


Asn Lys Asn Leu Cys Tyr Ala Asn Thr Ile Asn Trp Lys Lys Leu Phe 
                165                 170                 175     


Gly Thr Ser Gly Gln Lys Thr Lys Ile Ile Ser Asn Arg Gly Glu Asn 
            180                 185                 190         


Ser Cys Lys Ala Thr Gly Gln Val Cys His Ala Leu Cys Ser Pro Glu 
        195                 200                 205             


Gly Cys Trp Gly Pro Glu Pro Arg Asp Cys Val Ser Cys Arg Asn Val 
    210                 215                 220                 


Ser Arg Gly Arg Glu Cys Val Asp Lys Cys Asn Leu Leu Glu Gly Glu 
225                 230                 235                 240 


Pro Arg Glu Phe Val Glu Asn Ser Glu Cys Ile Gln Cys His Pro Glu 
                245                 250                 255     


Cys Leu Pro Gln Ala Met Asn Ile Thr Cys Thr Gly Arg Gly Pro Asp 
            260                 265                 270         


Asn Cys Ile Gln Cys Ala His Tyr Ile Asp Gly Pro His Cys Val Lys 
        275                 280                 285             


Thr Cys Pro Ala Gly Val Met Gly Glu Asn Asn Thr Leu Val Trp Lys 
    290                 295                 300                 


Tyr Ala Asp Ala Gly His Val Cys His Leu Cys His Pro Asn Cys Thr 
305                 310                 315                 320 


Tyr Gly Cys Thr Gly Pro Gly Leu Glu Gly Cys Pro Thr Asn Gly Pro 
                325                 330                 335     


Lys Ile Pro Ser Ile Ala Thr Gly Met Val Gly Ala Leu Leu Leu Leu 
            340                 345                 350         


Leu Val Val Ala Leu Gly Ile Gly Leu Phe Met Lys Arg Trp Asn Arg 
        355                 360                 365             


Gly Ile Leu Ala Ser Tyr Gln Pro Leu Ser Gln Ile Lys Arg Leu Leu 
    370                 375                 380                 


Ser Asp Ser Phe Leu Phe Asp Asn Pro Val Tyr Ala Ser 
385                 390                 395         


<210>  32
<211>  1194
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  nucleotide sequence encoding modified EGFRt (with NGFR signal 
       peptide; and EGFR cytoplasmic domain and recycling signal)

<400>  32
atgggagctg gtgctaccgg cagagctatg gatggaccta gactgctgct cctgctgctg       60

ctcggagttt ctcttggcgg agccagaaaa gtgtgcaacg gcatcggcat cggagagttc      120

aaggacagcc tgagcatcaa cgccaccaac atcaagcact tcaagaactg caccagcatc      180

agcggcgacc tgcacattct gcctgtggcc tttagaggcg acagcttcac ccacacacct      240

ccactggatc cccaagagct ggacatcctg aaaaccgtga aagagatcac cggatttctg      300

ttgatccagg cttggcccga gaaccggaca gatctgcacg ccttcgagaa cctggaaatc      360

atcagaggcc ggaccaagca gcacggccag ttttctctgg ctgtggtgtc cctgaacatc      420

accagcctgg gcctgagaag cctgaaagaa atcagcgacg gcgacgtgat catctccggc      480

aacaagaacc tgtgctacgc caacaccatc aactggaaga agctgttcgg caccagcggc      540

cagaaaacaa agatcatcag caaccggggc gagaacagct gcaaggctac aggccaagtg      600

tgccacgctc tgtgtagccc tgaaggctgt tggggacccg agcctagaga ttgcgtgtcc      660

tgcagaaacg tgtcccgggg cagagaatgc gtggacaagt gcaatctgct ggaaggcgag      720

ccccgcgagt tcgtggaaaa cagcgagtgc atccagtgtc accccgagtg tctgccccag      780

gccatgaaca ttacctgtac cggcagaggc cccgacaact gtattcagtg cgcccactac      840

atcgacggcc ctcactgcgt gaaaacatgt cctgctggcg tgatgggaga gaacaacacc      900

ctcgtgtgga agtatgccga cgccggacat gtgtgccacc tgtgtcaccc taattgcacc      960

tacggctgta caggccctgg cctggaaggc tgtccaacaa acggacctaa gatcccctct     1020

atcgccaccg gcatggttgg agccctgctg cttctgctgg tggtggccct tggaatcggc     1080

ctgtttatgc ggcggagaca catcgtgcgg aaggctagct atcagcccct gagccagatc     1140

aagagactgc tgagcgactc cttcctgttc gacaaccccg tgtacgctag ctga           1194


<210>  33
<211>  397
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  modified EGFRt (with NGFR signal peptide; and EGFR cytoplasmic 
       domain and recycling signal)

<400>  33

Met Gly Ala Gly Ala Thr Gly Arg Ala Met Asp Gly Pro Arg Leu Leu 
1               5                   10                  15      


Leu Leu Leu Leu Leu Gly Val Ser Leu Gly Gly Ala Arg Lys Val Cys 
            20                  25                  30          


Asn Gly Ile Gly Ile Gly Glu Phe Lys Asp Ser Leu Ser Ile Asn Ala 
        35                  40                  45              


Thr Asn Ile Lys His Phe Lys Asn Cys Thr Ser Ile Ser Gly Asp Leu 
    50                  55                  60                  


His Ile Leu Pro Val Ala Phe Arg Gly Asp Ser Phe Thr His Thr Pro 
65                  70                  75                  80  


Pro Leu Asp Pro Gln Glu Leu Asp Ile Leu Lys Thr Val Lys Glu Ile 
                85                  90                  95      


Thr Gly Phe Leu Leu Ile Gln Ala Trp Pro Glu Asn Arg Thr Asp Leu 
            100                 105                 110         


His Ala Phe Glu Asn Leu Glu Ile Ile Arg Gly Arg Thr Lys Gln His 
        115                 120                 125             


Gly Gln Phe Ser Leu Ala Val Val Ser Leu Asn Ile Thr Ser Leu Gly 
    130                 135                 140                 


Leu Arg Ser Leu Lys Glu Ile Ser Asp Gly Asp Val Ile Ile Ser Gly 
145                 150                 155                 160 


Asn Lys Asn Leu Cys Tyr Ala Asn Thr Ile Asn Trp Lys Lys Leu Phe 
                165                 170                 175     


Gly Thr Ser Gly Gln Lys Thr Lys Ile Ile Ser Asn Arg Gly Glu Asn 
            180                 185                 190         


Ser Cys Lys Ala Thr Gly Gln Val Cys His Ala Leu Cys Ser Pro Glu 
        195                 200                 205             


Gly Cys Trp Gly Pro Glu Pro Arg Asp Cys Val Ser Cys Arg Asn Val 
    210                 215                 220                 


Ser Arg Gly Arg Glu Cys Val Asp Lys Cys Asn Leu Leu Glu Gly Glu 
225                 230                 235                 240 


Pro Arg Glu Phe Val Glu Asn Ser Glu Cys Ile Gln Cys His Pro Glu 
                245                 250                 255     


Cys Leu Pro Gln Ala Met Asn Ile Thr Cys Thr Gly Arg Gly Pro Asp 
            260                 265                 270         


Asn Cys Ile Gln Cys Ala His Tyr Ile Asp Gly Pro His Cys Val Lys 
        275                 280                 285             


Thr Cys Pro Ala Gly Val Met Gly Glu Asn Asn Thr Leu Val Trp Lys 
    290                 295                 300                 


Tyr Ala Asp Ala Gly His Val Cys His Leu Cys His Pro Asn Cys Thr 
305                 310                 315                 320 


Tyr Gly Cys Thr Gly Pro Gly Leu Glu Gly Cys Pro Thr Asn Gly Pro 
                325                 330                 335     


Lys Ile Pro Ser Ile Ala Thr Gly Met Val Gly Ala Leu Leu Leu Leu 
            340                 345                 350         


Leu Val Val Ala Leu Gly Ile Gly Leu Phe Met Arg Arg Arg His Ile 
        355                 360                 365             


Val Arg Lys Ala Ser Tyr Gln Pro Leu Ser Gln Ile Lys Arg Leu Leu 
    370                 375                 380                 


Ser Asp Ser Phe Leu Phe Asp Asn Pro Val Tyr Ala Ser 
385                 390                 395         


<210>  34
<211>  1218
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  nucleotide sequence encoding modified EGFRt (with GMS SFR alpha 
       signal peptide; and NGFR transmembrane and cytoplasmic domain and
       recycling signal)

<400>  34
atgctgctgc tggtcacctc tctgctgctg tgcgaactgc cccatcctgc ctttctgctg       60

atccccagaa aagtgtgcaa cggcatcggc atcggagagt tcaaggacag cctgagcatc      120

aacgccacca acatcaagca cttcaagaac tgcaccagca tcagcggcga cctgcacatt      180

ctgcctgtgg cctttagagg cgacagcttc acccacacac ctccactgga tccccaagag      240

ctggacatcc tgaaaaccgt gaaagagatc accggatttc tgttgatcca ggcttggccc      300

gagaaccgga cagatctgca cgccttcgag aacctggaaa tcatcagagg ccggaccaag      360

cagcacggcc agttttctct ggctgtggtg tccctgaaca tcaccagcct gggcctgaga      420

agcctgaaag aaatcagcga cggcgacgtg atcatctccg gcaacaagaa cctgtgctac      480

gccaacacca tcaactggaa gaagctgttc ggcaccagcg gccagaaaac aaagatcatc      540

agcaaccggg gcgagaacag ctgcaaggct acaggccaag tgtgccacgc tctgtgtagc      600

cctgaaggct gttggggacc cgagcctaga gattgcgtgt cctgcagaaa cgtgtcccgg      660

ggcagagaat gcgtggacaa gtgcaatctg ctggaaggcg agccccgcga gttcgtggaa      720

aacagcgagt gcatccagtg tcaccccgag tgtctgcccc aggccatgaa cattacctgt      780

accggcagag gccccgacaa ctgtattcag tgcgcccact acatcgacgg ccctcactgc      840

gtgaaaacat gtcctgctgg cgtgatggga gagaacaaca ccctcgtgtg gaagtatgcc      900

gacgccggac atgtgtgcca cctgtgtcac cctaattgca cctacggctg tacaggccct      960

ggcctggaag gctgtccaac aaacggacct aagatcccct ctggcggcgg aggatctggc     1020

ggaggtggaa gcggaggcgg tggatctctg atccccgtgt actgtagcat cctggccgcc     1080

gtggttgtgg gactcgtggc ctatatcgcc ttcaagcggt ggaaccgggg catcctggct     1140

agctatcagc ctctgagcca gatcaagcgg ctgctgagcg actccttcct gttcgacaac     1200

cctgtgtacg ctagctga                                                   1218


<210>  35
<211>  405
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  modified EGFRt (with GMS SFR alpha signal peptide; and NGFR 
       transmembrane and cytoplasmic domain and recycling signal)

<400>  35

Met Leu Leu Leu Val Thr Ser Leu Leu Leu Cys Glu Leu Pro His Pro 
1               5                   10                  15      


Ala Phe Leu Leu Ile Pro Arg Lys Val Cys Asn Gly Ile Gly Ile Gly 
            20                  25                  30          


Glu Phe Lys Asp Ser Leu Ser Ile Asn Ala Thr Asn Ile Lys His Phe 
        35                  40                  45              


Lys Asn Cys Thr Ser Ile Ser Gly Asp Leu His Ile Leu Pro Val Ala 
    50                  55                  60                  


Phe Arg Gly Asp Ser Phe Thr His Thr Pro Pro Leu Asp Pro Gln Glu 
65                  70                  75                  80  


Leu Asp Ile Leu Lys Thr Val Lys Glu Ile Thr Gly Phe Leu Leu Ile 
                85                  90                  95      


Gln Ala Trp Pro Glu Asn Arg Thr Asp Leu His Ala Phe Glu Asn Leu 
            100                 105                 110         


Glu Ile Ile Arg Gly Arg Thr Lys Gln His Gly Gln Phe Ser Leu Ala 
        115                 120                 125             


Val Val Ser Leu Asn Ile Thr Ser Leu Gly Leu Arg Ser Leu Lys Glu 
    130                 135                 140                 


Ile Ser Asp Gly Asp Val Ile Ile Ser Gly Asn Lys Asn Leu Cys Tyr 
145                 150                 155                 160 


Ala Asn Thr Ile Asn Trp Lys Lys Leu Phe Gly Thr Ser Gly Gln Lys 
                165                 170                 175     


Thr Lys Ile Ile Ser Asn Arg Gly Glu Asn Ser Cys Lys Ala Thr Gly 
            180                 185                 190         


Gln Val Cys His Ala Leu Cys Ser Pro Glu Gly Cys Trp Gly Pro Glu 
        195                 200                 205             


Pro Arg Asp Cys Val Ser Cys Arg Asn Val Ser Arg Gly Arg Glu Cys 
    210                 215                 220                 


Val Asp Lys Cys Asn Leu Leu Glu Gly Glu Pro Arg Glu Phe Val Glu 
225                 230                 235                 240 


Asn Ser Glu Cys Ile Gln Cys His Pro Glu Cys Leu Pro Gln Ala Met 
                245                 250                 255     


Asn Ile Thr Cys Thr Gly Arg Gly Pro Asp Asn Cys Ile Gln Cys Ala 
            260                 265                 270         


His Tyr Ile Asp Gly Pro His Cys Val Lys Thr Cys Pro Ala Gly Val 
        275                 280                 285             


Met Gly Glu Asn Asn Thr Leu Val Trp Lys Tyr Ala Asp Ala Gly His 
    290                 295                 300                 


Val Cys His Leu Cys His Pro Asn Cys Thr Tyr Gly Cys Thr Gly Pro 
305                 310                 315                 320 


Gly Leu Glu Gly Cys Pro Thr Asn Gly Pro Lys Ile Pro Ser Gly Gly 
                325                 330                 335     


Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Leu Ile Pro 
            340                 345                 350         


Val Tyr Cys Ser Ile Leu Ala Ala Val Val Val Gly Leu Val Ala Tyr 
        355                 360                 365             


Ile Ala Phe Lys Arg Trp Asn Arg Gly Ile Leu Ala Ser Tyr Gln Pro 
    370                 375                 380                 


Leu Ser Gln Ile Lys Arg Leu Leu Ser Asp Ser Phe Leu Phe Asp Asn 
385                 390                 395                 400 


Pro Val Tyr Ala Ser 
                405 


<210>  36
<211>  3477
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  unmodified EGFRt

<400>  36
tttacatgtg ctcttaatta cagcagaacc ggtctgacct cttctcttcc tcccacagat       60

cgaggacgag agaaacctgc acgaggactt cgtgttcatg aagaccatcc agcggtgcaa      120

caccggcgag agaagtctga gcctgctgaa ctgcgaggaa atcaagagcc agttcgaggg      180

cttcgtgaag gacatcatgc tgaacaaaga ggaaacgaag aaagaaaact ccttcgagat      240

gcagaagggc gaccagaatc ctcagatcgc cgctcacgtg atcagcgagg ccagcagcaa      300

gacaacaagc gtgctgcagt gggccgagaa gggctactac accatgagca acaacctggt      360

caccctggaa aacggcaagc agctgacagt gaagcggcag ggcctgtact acatctacgc      420

ccaagtgacc ttctgcagca acagagaggc cagctctcag gcccctttta tcgccagcct      480

gtgcctgaag tcccctggca gattcgagcg gattctgctg agagccgcca acacacacag      540

cagcgccaaa ccttgtggcc agcagtctat tcacctcggc ggagtgtttg agctgcagcc      600

tggcgcaagc gtgttcgtga atgtgacaga ccctagccag gtgtcccacg gcaccggctt      660

tacatctttc ggactgctga agctgtgaac agtgtcacct tgcaggctgt ggtggagctg      720

acgctgggag tcttcataat acagcacagc ggttaagccc accccctgtt aactgcctat      780

ttataaccct agggaattaa ctcgaggaat tccgcccctc tccctccccc ccccctaacg      840

ttactggccg aagccgcttg gaataaggcc ggtgtgcgtt tgtctatatg ttattttcca      900

ccatattgcc gtcttttggc aatgtgaggg cccggaaacc tggccctgtc ttcttgacga      960

gcattctagg ggtctttccc ctctcgccaa aggaatgcaa ggtctgttga atgtcgtgaa     1020

ggaagcagtt cctctggaag cttcttgaag acaaacaacg tctgtagcga ccctttgcag     1080

gcagcggaac cccccacctg gcgacaggtg cctctgcggc caaaagccaa cgtgtataag     1140

atacacctgc aaaggcggca caaccccagt gccacgttgt gagttggata gttgtggaaa     1200

gagtcaaatg gctctcctca agcgtattca acaaggggct gaaggatgcc cagaaggtac     1260

cccattgtat gggatctgat ctggggcctc ggtgcacatg ctttacatgt gtttagtcga     1320

ggttaaaaaa cgtctaggcc ccccgaacca cggggacgtg gttttccttt gaaaaacacg     1380

atgataatat ggccacaacc atgctgctgc tggtcacctc tctgctgctg tgcgaactgc     1440

cccatcctgc ctttctgctg atccccagaa aagtgtgcaa cggcatcggc atcggagagt     1500

tcaaggacag cctgagcatc aacgccacca acatcaagca cttcaagaac tgcaccagca     1560

tcagcggcga cctgcacatt ctgcctgtgg cctttagagg cgacagcttc acccacacac     1620

ctccactgga tccccaagag ctggacatcc tgaaaaccgt gaaagagatc accggatttc     1680

tgttgatcca ggcttggccc gagaaccgga cagatctgca cgccttcgag aacctggaaa     1740

tcatcagagg ccggaccaag cagcacggcc agttttctct ggctgtggtg tccctgaaca     1800

tcaccagcct gggcctgaga agcctgaaag aaatcagcga cggcgacgtg atcatctccg     1860

gcaacaagaa cctgtgctac gccaacacca tcaactggaa gaagctgttc ggcaccagcg     1920

gccagaaaac aaagatcatc agcaaccggg gcgagaacag ctgcaaggct acaggccaag     1980

tgtgccacgc tctgtgtagc cctgaaggct gttggggacc cgagcctaga gattgcgtgt     2040

cctgcagaaa cgtgtcccgg ggcagagaat gcgtggacaa gtgcaatctg ctggaaggcg     2100

agccccgcga gttcgtggaa aacagcgagt gcatccagtg tcaccccgag tgtctgcccc     2160

aggccatgaa cattacctgt accggcagag gccccgacaa ctgtattcag tgcgcccact     2220

acatcgacgg ccctcactgc gtgaaaacat gtcctgctgg cgtgatggga gagaacaaca     2280

ccctcgtgtg gaagtatgcc gacgccggac atgtgtgcca cctgtgtcac cctaattgca     2340

cctacggctg tacaggccct ggcctggaag gctgtccaac aaacggacct aagatcccct     2400

ctatcgccac cggcatggtt ggagccctgc tgcttctgct ggtggtggcc cttggaatcg     2460

gcctgtttat gtagcctagg atcctcctta tggagaacta tttattatac actccaaggc     2520

atgtagaact gtaataagtg aattacaggt cacatgaaac caaaacgggc cctgctccat     2580

aagagcttat atatctgaag cagcaacccc actgatgcag acatccagag agtcctatga     2640

aaagacaagg ccattatgca caggttgaat tctgagtaaa cagcagataa cttgccaagt     2700

tcagttttgt ttctttgcgt gcagtgtctt tccatggata atgcatttga tttatcagtg     2760

aagatgcaga agggaaatgg ggagcctcag ctcacattca gttatggttg actctgggtt     2820

cctatggcct tgttggaggg ggccaggctc tagaacgtct aacacagtgg agaaccgaaa     2880

cccccccccc ccgccaccct ctcggacagt tattcattct ctttcaatct ctctctctcc     2940

atctctctct ttcagtctct ctctctcaac ctctttcttc caatctctct ttctcaatct     3000

ctctgtttcc ctttgtcagt ctcttccctc ccccagtctc tcttctcaat ccccctttct     3060

aacacacaca cacacacaca cacacacaca cacacacaca cacacacaca cacagagtca     3120

ggccgttgct agtcagttct cttctttcca ccctgtccct atctctacca ctatagatga     3180

gggtgaggag tagggagtgc agccctgagc ctgcccactc ctcattacga aatgactgta     3240

tttaaaggaa atctattgta tctacgatgt ctccattgtt tccagagtga acttgtaatt     3300

atcttgttat ttattttttg aataataaag acctcttaac attacgcgct taacattatc     3360

gttgttgttt gagtacctaa agctcccagc caggttgggg aaagaggaag catttggagg     3420

gaattttccc aacctttgtg atgttttcat aaactttgtt ctcaagctac ttacatt        3477


<210>  37
<211>  3525
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  modified EGFRt with NGFR signal peptide and NGFR cytoplasmic 
       domain

<400>  37
tttacatgtg ctcttaatta cagcagaacc ggtctgacct cttctcttcc tcccacagat       60

cgaggacgag agaaacctgc acgaggactt cgtgttcatg aagaccatcc agcggtgcaa      120

caccggcgag agaagtctga gcctgctgaa ctgcgaggaa atcaagagcc agttcgaggg      180

cttcgtgaag gacatcatgc tgaacaaaga ggaaacgaag aaagaaaact ccttcgagat      240

gcagaagggc gaccagaatc ctcagatcgc cgctcacgtg atcagcgagg ccagcagcaa      300

gacaacaagc gtgctgcagt gggccgagaa gggctactac accatgagca acaacctggt      360

caccctggaa aacggcaagc agctgacagt gaagcggcag ggcctgtact acatctacgc      420

ccaagtgacc ttctgcagca acagagaggc cagctctcag gcccctttta tcgccagcct      480

gtgcctgaag tcccctggca gattcgagcg gattctgctg agagccgcca acacacacag      540

cagcgccaaa ccttgtggcc agcagtctat tcacctcggc ggagtgtttg agctgcagcc      600

tggcgcaagc gtgttcgtga atgtgacaga ccctagccag gtgtcccacg gcaccggctt      660

tacatctttc ggactgctga agctgtgaac agtgtcacct tgcaggctgt ggtggagctg      720

acgctgggag tcttcataat acagcacagc ggttaagccc accccctgtt aactgcctat      780

ttataaccct agggaattaa ctcgaggaat tccgcccctc tccctccccc ccccctaacg      840

ttactggccg aagccgcttg gaataaggcc ggtgtgcgtt tgtctatatg ttattttcca      900

ccatattgcc gtcttttggc aatgtgaggg cccggaaacc tggccctgtc ttcttgacga      960

gcattctagg ggtctttccc ctctcgccaa aggaatgcaa ggtctgttga atgtcgtgaa     1020

ggaagcagtt cctctggaag cttcttgaag acaaacaacg tctgtagcga ccctttgcag     1080

gcagcggaac cccccacctg gcgacaggtg cctctgcggc caaaagccaa cgtgtataag     1140

atacacctgc aaaggcggca caaccccagt gccacgttgt gagttggata gttgtggaaa     1200

gagtcaaatg gctctcctca agcgtattca acaaggggct gaaggatgcc cagaaggtac     1260

cccattgtat gggatctgat ctggggcctc ggtgcacatg ctttacatgt gtttagtcga     1320

ggttaaaaaa cgtctaggcc ccccgaacca cggggacgtg gttttccttt gaaaaacacg     1380

atgataatat ggccacaacc atgggagctg gtgctaccgg cagagctatg gatggaccta     1440

gactgctgct cctgctgctg ctcggagttt ctcttggcgg agccagaaaa gtgtgcaacg     1500

gcatcggcat cggagagttc aaggacagcc tgagcatcaa cgccaccaac atcaagcact     1560

tcaagaactg caccagcatc agcggcgacc tgcacattct gcctgtggcc tttagaggcg     1620

acagcttcac ccacacacct ccactggatc cccaagagct ggacatcctg aaaaccgtga     1680

aagagatcac cggatttctg ttgatccagg cttggcccga gaaccggaca gatctgcacg     1740

ccttcgagaa cctggaaatc atcagaggcc ggaccaagca gcacggccag ttttctctgg     1800

ctgtggtgtc cctgaacatc accagcctgg gcctgagaag cctgaaagaa atcagcgacg     1860

gcgacgtgat catctccggc aacaagaacc tgtgctacgc caacaccatc aactggaaga     1920

agctgttcgg caccagcggc cagaaaacaa agatcatcag caaccggggc gagaacagct     1980

gcaaggctac aggccaagtg tgccacgctc tgtgtagccc tgaaggctgt tggggacccg     2040

agcctagaga ttgcgtgtcc tgcagaaacg tgtcccgggg cagagaatgc gtggacaagt     2100

gcaatctgct ggaaggcgag ccccgcgagt tcgtggaaaa cagcgagtgc atccagtgtc     2160

accccgagtg tctgccccag gccatgaaca ttacctgtac cggcagaggc cccgacaact     2220

gtattcagtg cgcccactac atcgacggcc ctcactgcgt gaaaacatgt cctgctggcg     2280

tgatgggaga gaacaacacc ctcgtgtgga agtatgccga cgccggacat gtgtgccacc     2340

tgtgtcaccc taattgcacc tacggctgta caggccctgg cctggaaggc tgtccaacaa     2400

acggacctaa gatcccctct atcgccaccg gcatggttgg agccctgctg cttctgctgg     2460

tggtggccct tggaatcggc ctgtttatga agcggtggaa ccggggcatc ctggctagct     2520

gacctaggat cctccttatg gagaactatt tattatacac tccaaggcat gtagaactgt     2580

aataagtgaa ttacaggtca catgaaacca aaacgggccc tgctccataa gagcttatat     2640

atctgaagca gcaaccccac tgatgcagac atccagagag tcctatgaaa agacaaggcc     2700

attatgcaca ggttgaattc tgagtaaaca gcagataact tgccaagttc agttttgttt     2760

ctttgcgtgc agtgtctttc catggataat gcatttgatt tatcagtgaa gatgcagaag     2820

ggaaatgggg agcctcagct cacattcagt tatggttgac tctgggttcc tatggccttg     2880

ttggaggggg ccaggctcta gaacgtctaa cacagtggag aaccgaaacc cccccccccc     2940

gccaccctct cggacagtta ttcattctct ttcaatctct ctctctccat ctctctcttt     3000

cagtctctct ctctcaacct ctttcttcca atctctcttt ctcaatctct ctgtttccct     3060

ttgtcagtct cttccctccc ccagtctctc ttctcaatcc ccctttctaa cacacacaca     3120

cacacacaca cacacacaca cacacacaca cacacacaca cagagtcagg ccgttgctag     3180

tcagttctct tctttccacc ctgtccctat ctctaccact atagatgagg gtgaggagta     3240

gggagtgcag ccctgagcct gcccactcct cattacgaaa tgactgtatt taaaggaaat     3300

ctattgtatc tacgatgtct ccattgtttc cagagtgaac ttgtaattat cttgttattt     3360

attttttgaa taataaagac ctcttaacat tacgcgctta acattatcgt tgttgtttga     3420

gtacctaaag ctcccagcca ggttggggaa agaggaagca tttggaggga attttcccaa     3480

cctttgtgat gttttcataa actttgttct caagctactt acatt                     3525


<210>  38
<211>  3525
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  modified EGFRt with NGFR signal peptide and EGFR cytoplasmic 
       domain

<400>  38
tttacatgtg ctcttaatta cagcagaacc ggtctgacct cttctcttcc tcccacagat       60

cgaggacgag agaaacctgc acgaggactt cgtgttcatg aagaccatcc agcggtgcaa      120

caccggcgag agaagtctga gcctgctgaa ctgcgaggaa atcaagagcc agttcgaggg      180

cttcgtgaag gacatcatgc tgaacaaaga ggaaacgaag aaagaaaact ccttcgagat      240

gcagaagggc gaccagaatc ctcagatcgc cgctcacgtg atcagcgagg ccagcagcaa      300

gacaacaagc gtgctgcagt gggccgagaa gggctactac accatgagca acaacctggt      360

caccctggaa aacggcaagc agctgacagt gaagcggcag ggcctgtact acatctacgc      420

ccaagtgacc ttctgcagca acagagaggc cagctctcag gcccctttta tcgccagcct      480

gtgcctgaag tcccctggca gattcgagcg gattctgctg agagccgcca acacacacag      540

cagcgccaaa ccttgtggcc agcagtctat tcacctcggc ggagtgtttg agctgcagcc      600

tggcgcaagc gtgttcgtga atgtgacaga ccctagccag gtgtcccacg gcaccggctt      660

tacatctttc ggactgctga agctgtgaac agtgtcacct tgcaggctgt ggtggagctg      720

acgctgggag tcttcataat acagcacagc ggttaagccc accccctgtt aactgcctat      780

ttataaccct agggaattaa ctcgaggaat tccgcccctc tccctccccc ccccctaacg      840

ttactggccg aagccgcttg gaataaggcc ggtgtgcgtt tgtctatatg ttattttcca      900

ccatattgcc gtcttttggc aatgtgaggg cccggaaacc tggccctgtc ttcttgacga      960

gcattctagg ggtctttccc ctctcgccaa aggaatgcaa ggtctgttga atgtcgtgaa     1020

ggaagcagtt cctctggaag cttcttgaag acaaacaacg tctgtagcga ccctttgcag     1080

gcagcggaac cccccacctg gcgacaggtg cctctgcggc caaaagccaa cgtgtataag     1140

atacacctgc aaaggcggca caaccccagt gccacgttgt gagttggata gttgtggaaa     1200

gagtcaaatg gctctcctca agcgtattca acaaggggct gaaggatgcc cagaaggtac     1260

cccattgtat gggatctgat ctggggcctc ggtgcacatg ctttacatgt gtttagtcga     1320

ggttaaaaaa cgtctaggcc ccccgaacca cggggacgtg gttttccttt gaaaaacacg     1380

atgataatat ggccacaacc atgggagctg gtgctaccgg cagagctatg gatggaccta     1440

gactgctgct cctgctgctg ctcggagttt ctcttggcgg agccagaaaa gtgtgcaacg     1500

gcatcggcat cggagagttc aaggacagcc tgagcatcaa cgccaccaac atcaagcact     1560

tcaagaactg caccagcatc agcggcgacc tgcacattct gcctgtggcc tttagaggcg     1620

acagcttcac ccacacacct ccactggatc cccaagagct ggacatcctg aaaaccgtga     1680

aagagatcac cggatttctg ttgatccagg cttggcccga gaaccggaca gatctgcacg     1740

ccttcgagaa cctggaaatc atcagaggcc ggaccaagca gcacggccag ttttctctgg     1800

ctgtggtgtc cctgaacatc accagcctgg gcctgagaag cctgaaagaa atcagcgacg     1860

gcgacgtgat catctccggc aacaagaacc tgtgctacgc caacaccatc aactggaaga     1920

agctgttcgg caccagcggc cagaaaacaa agatcatcag caaccggggc gagaacagct     1980

gcaaggctac aggccaagtg tgccacgctc tgtgtagccc tgaaggctgt tggggacccg     2040

agcctagaga ttgcgtgtcc tgcagaaacg tgtcccgggg cagagaatgc gtggacaagt     2100

gcaatctgct ggaaggcgag ccccgcgagt tcgtggaaaa cagcgagtgc atccagtgtc     2160

accccgagtg tctgccccag gccatgaaca ttacctgtac cggcagaggc cccgacaact     2220

gtattcagtg cgcccactac atcgacggcc ctcactgcgt gaaaacatgt cctgctggcg     2280

tgatgggaga gaacaacacc ctcgtgtgga agtatgccga cgccggacat gtgtgccacc     2340

tgtgtcaccc taattgcacc tacggctgta caggccctgg cctggaaggc tgtccaacaa     2400

acggacctaa gatcccctct atcgccaccg gcatggttgg agccctgctg cttctgctgg     2460

tggtggccct tggaatcggc ctgtttatgc ggcggagaca catcgtgcgg aaggctagct     2520

gacctaggat cctccttatg gagaactatt tattatacac tccaaggcat gtagaactgt     2580

aataagtgaa ttacaggtca catgaaacca aaacgggccc tgctccataa gagcttatat     2640

atctgaagca gcaaccccac tgatgcagac atccagagag tcctatgaaa agacaaggcc     2700

attatgcaca ggttgaattc tgagtaaaca gcagataact tgccaagttc agttttgttt     2760

ctttgcgtgc agtgtctttc catggataat gcatttgatt tatcagtgaa gatgcagaag     2820

ggaaatgggg agcctcagct cacattcagt tatggttgac tctgggttcc tatggccttg     2880

ttggaggggg ccaggctcta gaacgtctaa cacagtggag aaccgaaacc cccccccccc     2940

gccaccctct cggacagtta ttcattctct ttcaatctct ctctctccat ctctctcttt     3000

cagtctctct ctctcaacct ctttcttcca atctctcttt ctcaatctct ctgtttccct     3060

ttgtcagtct cttccctccc ccagtctctc ttctcaatcc ccctttctaa cacacacaca     3120

cacacacaca cacacacaca cacacacaca cacacacaca cagagtcagg ccgttgctag     3180

tcagttctct tctttccacc ctgtccctat ctctaccact atagatgagg gtgaggagta     3240

gggagtgcag ccctgagcct gcccactcct cattacgaaa tgactgtatt taaaggaaat     3300

ctattgtatc tacgatgtct ccattgtttc cagagtgaac ttgtaattat cttgttattt     3360

attttttgaa taataaagac ctcttaacat tacgcgctta acattatcgt tgttgtttga     3420

gtacctaaag ctcccagcca ggttggggaa agaggaagca tttggaggga attttcccaa     3480

cctttgtgat gttttcataa actttgttct caagctactt acatt                     3525


<210>  39
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  cytoplasmic tail sequence

<400>  39

Lys Arg Trp Asn Arg Gly Ile Leu 
1               5               


<210>  40
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  cytoplasmic tail sequence

<400>  40

Arg Arg Arg His Ile Val Arg Lys 
1               5               


<210>  41
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  cytoplasmic tail sequence

<400>  41

Lys Arg Trp Asn Arg Gly Ile Leu Ala Ser 
1               5                   10  


<210>  42
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  cytoplasmic tail sequence

<400>  42

Arg Arg Arg His Ile Val Arg Lys Ala Ser 
1               5                   10  


<210>  43
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  example nucleotide sequence encoding a cytoplasmic tail

<400>  43
aagcggtgga accggggcat cctggctagc                                        30


<210>  44
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  example nucleotide sequence encoding a cytoplasmic tail

<400>  44
cggcggagac acatcgtgcg gaaggctagc                                        30


