               SEQUENCE LISTING

<110> Nordic Bioscience A/S

<120> Calprotectin Assay

<130> P21320WO

<140> EPPCT/2021/062753
<141> 2021-05-12

<150> GB2100902.2
<151> 2021-01-22
<150> GB2007087.6
<151> 2020-05-13

<160> 48

<170> BiSSAP 1.3.6

<210> 1
<211> 17
<212> PRT
<213> Artificial Sequence


<220> 
<223> Synthetic peptide

<400> 1
Lys Leu Gly His Pro Asp Thr Leu Asn Gln Gly Glu Phe Lys Glu Leu 
1               5                   10                  15      
Val 
    

<210> 2
<211> 17
<212> PRT
<213> Artificial Sequence


<220> 
<223> Synthetic peptide

<400> 2
Arg Lys Asp Leu Gln Asn Phe Leu Lys Lys Glu Asn Lys Asn Glu Lys 
1               5                   10                  15      
Val 
    

<210> 3
<211> 18
<212> PRT
<213> Artificial Sequence


<220> 
<223> Synthetic peptide

<400> 3
Arg Lys Asp Leu Gln Asn Phe Leu Lys Lys Glu Asn Lys Asn Glu Lys 
1               5                   10                  15      
Val Ile 
        

<210> 4
<211> 15
<212> PRT
<213> Artificial Sequence


<220> 
<223> Synthetic peptide

<400> 4
Glu His Ile Met Glu Asp Leu Asp Thr Asn Ala Asp Lys Gln Leu 
1               5                   10                  15  

<210> 5
<211> 25
<212> PRT
<213> Artificial Sequence


<220> 
<223> Synthetic peptide

<400> 5
Ser His Glu Lys Met His Glu Gly Asp Glu Gly Pro Gly His His His 
1               5                   10                  15      
Lys Pro Gly Leu Gly Glu Gly Thr Pro 
            20                  25  

<210> 6
<211> 11
<212> PRT
<213> Artificial Sequence


<220> 
<223> Synthetic peptide

<400> 6
Tyr Arg Asp Asp Leu Lys Lys Leu Leu Glu Thr 
1               5                   10      

<210> 7
<211> 13
<212> PRT
<213> Artificial Sequence


<220> 
<223> Synthetic peptide

<400> 7
Trp Phe Lys Glu Leu Asp Ile Asn Thr Asp Gly Ala Val 
1               5                   10              

<210> 8
<211> 11
<212> PRT
<213> Artificial Sequence


<220> 
<223> Synthetic peptide

<400> 8
Val Lys Leu Gly His Pro Asp Thr Leu Asn Gln 
1               5                   10      

<210> 9
<211> 9
<212> PRT
<213> Artificial Sequence


<220> 
<223> Synthetic peptide

<400> 9
Leu Gly His Pro Asp Thr Leu Asn Gln 
1               5                   

<210> 10
<211> 11
<212> PRT
<213> Artificial Sequence


<220> 
<223> Synthetic peptide

<400> 10
Leu Asn Gln Gly Glu Phe Lys Glu Leu Val Arg 
1               5                   10      

<210> 11
<211> 9
<212> PRT
<213> Artificial Sequence


<220> 
<223> Synthetic peptide

<400> 11
Leu Asn Gln Gly Glu Phe Lys Glu Leu 
1               5                   

<210> 12
<211> 10
<212> PRT
<213> Artificial Sequence


<220> 
<223> Synthetic peptide

<400> 12
Lys Leu Gly His Pro Asp Thr Leu Asn Gln 
1               5                   10  

<210> 13
<211> 17
<212> PRT
<213> Artificial Sequence


<220> 
<223> Synthetic peptide

<400> 13
Lys Ser Ser Gln Ser Leu Leu Asn Ser Gly Asn Gln Lys Asn Tyr Leu 
1               5                   10                  15      
Ala 
    

<210> 14
<211> 7
<212> PRT
<213> Artificial Sequence


<220> 
<223> Synthetic peptide

<400> 14
Gly Ala Ser Thr Arg Glu Ser 
1               5           

<210> 15
<211> 9
<212> PRT
<213> Artificial Sequence


<220> 
<223> Synthetic peptide

<400> 15
Leu Asn Asp His Ser Tyr Pro Tyr Thr 
1               5                   

<210> 16
<211> 5
<212> PRT
<213> Artificial Sequence


<220> 
<223> Synthetic peptide

<400> 16
Asp His Val Ile Asn 
1               5   

<210> 17
<211> 17
<212> PRT
<213> Artificial Sequence


<220> 
<223> Synthetic peptide

<400> 17
Glu Ile Tyr Pro Gly Ser Gly Ser Thr Tyr Tyr Asn Glu Lys Phe Lys 
1               5                   10                  15      
Gly 
    

<210> 18
<211> 3
<212> PRT
<213> Artificial Sequence


<220> 
<223> Synthetic peptide

<400> 18
Phe Ala Tyr 
1           

<210> 19
<211> 80
<212> PRT
<213> Artificial Sequence


<220> 
<223> Synthetic peptide

<400> 19
Lys Ser Ser Gln Ser Leu Leu Asn Ser Gly Asn Gln Lys Asn Tyr Leu 
1               5                   10                  15      
Ala Trp Tyr Gln Gln Lys Pro Gly Gln Pro Pro Lys Leu Leu Ile Tyr 
            20                  25                  30          
Gly Ala Ser Thr Arg Glu Ser Gly Val Pro Asp Arg Phe Thr Gly Ser 
        35                  40                  45              
Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Val Gln Ala Glu 
    50                  55                  60                  
Asp Leu Ala Val Tyr Tyr Cys Leu Asn Asp His Ser Tyr Pro Tyr Thr 
65                  70                  75                  80  



<210> 20
<211> 71
<212> PRT
<213> Artificial Sequence


<220> 
<223> Synthetic peptide

<400> 20
Asp His Val Ile Asn Trp Val Arg Gln Arg Thr Gly Gln Gly Leu Glu 
1               5                   10                  15      
Trp Ile Gly Glu Ile Tyr Pro Gly Ser Gly Ser Thr Tyr Tyr Asn Glu 
            20                  25                  30          
Lys Phe Lys Gly Lys Ala Thr Leu Thr Ala Asp Lys Ser Ser Asn Thr 
        35                  40                  45              
Ala Tyr Met Gln Leu Ser Ser Leu Thr Ser Glu Asp Ser Ala Val Tyr 
    50                  55                  60                  
Phe Cys Ala Trp Phe Ala Tyr 
65                  70      

<210> 21
<211> 113
<212> PRT
<213> Artificial Sequence


<220> 
<223> Synthetic peptide

<400> 21
Asp Ile Val Met Thr Gln Ser Pro Ser Ser Leu Ser Val Ser Ala Gly 
1               5                   10                  15      
Glu Lys Val Thr Met Ser Cys Lys Ser Ser Gln Ser Leu Leu Asn Ser 
            20                  25                  30          
Gly Asn Gln Lys Asn Tyr Leu Ala Trp Tyr Gln Gln Lys Pro Gly Gln 
        35                  40                  45              
Pro Pro Lys Leu Leu Ile Tyr Gly Ala Ser Thr Arg Glu Ser Gly Val 
    50                  55                  60                  
Pro Asp Arg Phe Thr Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr 
65                  70                  75                  80  
Ile Ser Ser Val Gln Ala Glu Asp Leu Ala Val Tyr Tyr Cys Leu Asn 
                85                  90                  95      
Asp His Ser Tyr Pro Tyr Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile 
            100                 105                 110         
Lys 
    

<210> 22
<211> 112
<212> PRT
<213> Artificial Sequence


<220> 
<223> Synthetic peptide

<400> 22
Gln Val Gln Leu Gln Gln Ser Gly Pro Glu Leu Val Lys Pro Gly Ala 
1               5                   10                  15      
Ser Val Lys Met Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Asp His 
            20                  25                  30          
Val Ile Asn Trp Val Arg Gln Arg Thr Gly Gln Gly Leu Glu Trp Ile 
        35                  40                  45              
Gly Glu Ile Tyr Pro Gly Ser Gly Ser Thr Tyr Tyr Asn Glu Lys Phe 
    50                  55                  60                  
Lys Gly Lys Ala Thr Leu Thr Ala Asp Lys Ser Ser Asn Thr Ala Tyr 
65                  70                  75                  80  
Met Gln Leu Ser Ser Leu Thr Ser Glu Asp Ser Ala Val Tyr Phe Cys 
                85                  90                  95      
Ala Trp Phe Ala Tyr Trp Gly Gln Gly Thr Leu Val Thr Val Ser Ala 
            100                 105                 110         



<210> 23
<211> 10
<212> PRT
<213> Artificial Sequence


<220> 
<223> Synthetic peptide

<220> 
<221> MOD_RES
<222> 10
<223> Gln is biotinylated, optionally via a linker

<400> 23
Lys Leu Gly His Pro Asp Thr Leu Asn Gln 
1               5                   10  

<210> 24
<211> 13
<212> PRT
<213> Artificial Sequence


<220> 
<223> Synthetic peptide

<220> 
<221> MOD_RES
<222> 13
<223> Cys is bound to Keyhole Limpet Hemocyanin

<400> 24
Lys Leu Gly His Pro Asp Thr Leu Asn Gln Gly Gly Cys 
1               5                   10              

<210> 25
<211> 454
<212> PRT
<213> Artificial Sequence


<220> 
<223> Synthetic peptide

<400> 25
Met Glu Trp Arg Ile Phe Leu Phe Ile Leu Ser Gly Thr Ala Gly Val 
1               5                   10                  15      
His Ser Gln Val Gln Leu Gln Gln Ser Gly Pro Glu Leu Val Lys Pro 
            20                  25                  30          
Gly Ala Ser Val Lys Met Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr 
        35                  40                  45              
Asp His Val Ile Asn Trp Val Arg Gln Arg Thr Gly Gln Gly Leu Glu 
    50                  55                  60                  
Trp Ile Gly Glu Ile Tyr Pro Gly Ser Gly Ser Thr Tyr Tyr Asn Glu 
65                  70                  75                  80  
Lys Phe Lys Gly Lys Ala Thr Leu Thr Ala Asp Lys Ser Ser Asn Thr 
                85                  90                  95      
Ala Tyr Met Gln Leu Ser Ser Leu Thr Ser Glu Asp Ser Ala Val Tyr 
            100                 105                 110         
Phe Cys Ala Trp Phe Ala Tyr Trp Gly Gln Gly Thr Leu Val Thr Val 
        115                 120                 125             
Ser Ala Ala Lys Thr Thr Pro Pro Ser Val Tyr Pro Leu Ala Pro Gly 
    130                 135                 140                 
Ser Ala Ala Gln Thr Asn Ser Met Val Thr Leu Gly Cys Leu Val Lys 
145                 150                 155                 160 
Gly Tyr Phe Pro Glu Pro Val Thr Val Thr Trp Asn Ser Gly Ser Leu 
                165                 170                 175     
Ser Ser Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Asp Leu Tyr 
            180                 185                 190         
Thr Leu Ser Ser Ser Val Thr Val Pro Ser Ser Thr Trp Pro Ser Glu 
        195                 200                 205             
Thr Val Thr Cys Asn Val Ala His Pro Ala Ser Ser Thr Lys Val Asp 
    210                 215                 220                 
Lys Lys Ile Val Pro Arg Asp Cys Gly Cys Lys Pro Cys Ile Cys Thr 
225                 230                 235                 240 
Val Pro Glu Val Ser Ser Val Phe Ile Phe Pro Pro Lys Pro Lys Asp 
                245                 250                 255     
Val Leu Thr Ile Thr Leu Thr Pro Lys Val Thr Cys Val Val Val Asp 
            260                 265                 270         
Ile Ser Lys Asp Asp Pro Glu Val Gln Phe Ser Trp Phe Val Asp Asp 
        275                 280                 285             
Val Glu Val His Thr Ala Gln Thr Gln Pro Arg Glu Glu Gln Phe Asn 
    290                 295                 300                 
Ser Thr Phe Arg Ser Val Ser Glu Leu Pro Ile Met His Gln Asp Trp 
305                 310                 315                 320 
Leu Asn Gly Lys Glu Phe Lys Cys Arg Val Asn Ser Ala Ala Phe Pro 
                325                 330                 335     
Ala Pro Ile Glu Lys Thr Ile Ser Lys Thr Lys Gly Arg Pro Lys Ala 
            340                 345                 350         
Pro Gln Val Tyr Thr Ile Pro Pro Pro Lys Glu Gln Met Ala Lys Asp 
        355                 360                 365             
Lys Val Ser Leu Thr Cys Met Ile Thr Asp Phe Phe Pro Glu Asp Ile 
    370                 375                 380                 
Thr Val Glu Trp Gln Trp Asn Gly Gln Pro Ala Glu Asn Tyr Lys Asn 
385                 390                 395                 400 
Thr Gln Pro Ile Met Asp Thr Asp Gly Ser Tyr Phe Val Tyr Ser Lys 
                405                 410                 415     
Leu Asn Val Gln Lys Ser Asn Trp Glu Ala Gly Asn Thr Phe Thr Cys 
            420                 425                 430         
Ser Val Leu His Glu Gly Leu His Asn His His Thr Glu Lys Ser Leu 
        435                 440                 445             
Ser His Ser Pro Gly Lys 
    450                 

<210> 26
<211> 240
<212> PRT
<213> Artificial Sequence


<220> 
<223> Synthetic peptide

<400> 26
Met Glu Ser Gln Thr Gln Val Leu Ile Ser Leu Leu Phe Trp Val Ser 
1               5                   10                  15      
Gly Ala Cys Gly Asp Ile Val Met Thr Gln Ser Pro Ser Ser Leu Ser 
            20                  25                  30          
Val Ser Ala Gly Glu Lys Val Thr Met Ser Cys Lys Ser Ser Gln Ser 
        35                  40                  45              
Leu Leu Asn Ser Gly Asn Gln Lys Asn Tyr Leu Ala Trp Tyr Gln Gln 
    50                  55                  60                  
Lys Pro Gly Gln Pro Pro Lys Leu Leu Ile Tyr Gly Ala Ser Thr Arg 
65                  70                  75                  80  
Glu Ser Gly Val Pro Asp Arg Phe Thr Gly Ser Gly Ser Gly Thr Asp 
                85                  90                  95      
Phe Thr Leu Thr Ile Ser Ser Val Gln Ala Glu Asp Leu Ala Val Tyr 
            100                 105                 110         
Tyr Cys Leu Asn Asp His Ser Tyr Pro Tyr Thr Phe Gly Gly Gly Thr 
        115                 120                 125             
Lys Leu Glu Ile Lys Arg Ala Asp Ala Ala Pro Thr Val Ser Ile Phe 
    130                 135                 140                 
Pro Pro Ser Ser Glu Gln Leu Thr Ser Gly Gly Ala Ser Val Val Cys 
145                 150                 155                 160 
Phe Leu Asn Asn Phe Tyr Pro Lys Asp Ile Asn Val Lys Trp Lys Ile 
                165                 170                 175     
Asp Gly Ser Glu Arg Gln Asn Gly Val Leu Asn Ser Trp Thr Asp Gln 
            180                 185                 190         
Asp Ser Lys Asp Ser Thr Tyr Ser Met Ser Ser Thr Leu Thr Leu Thr 
        195                 200                 205             
Lys Asp Glu Tyr Glu Arg His Asn Ser Tyr Thr Cys Glu Ala Thr His 
    210                 215                 220                 
Lys Thr Ser Thr Ser Pro Ile Val Lys Ser Phe Asn Arg Asn Glu Cys 
225                 230                 235                 240 



<210> 27
<211> 11
<212> PRT
<213> Artificial Sequence


<220> 
<223> Synthetic peptide

<220> 
<221> MOD_RES
<222> 11
<223> Lys is biotinylated

<400> 27
Lys Leu Gly His Pro Asp Thr Leu Asn Gln Lys 
1               5                   10      

<210> 28
<211> 12
<212> PRT
<213> Artificial Sequence


<220> 
<223> Synthetic peptide

<400> 28
Met Glu Asp Leu Asp Thr Asn Ala Asp Lys Gln Leu 
1               5                   10          

<210> 29
<211> 16
<212> PRT
<213> Artificial Sequence


<220> 
<223> Synthetic peptide

<400> 29
Glu His Ile Met Glu Asp Leu Asp Thr Asn Ala Asp Lys Gln Leu Ser 
1               5                   10                  15      



<210> 30
<211> 10
<212> PRT
<213> Artificial Sequence


<220> 
<223> Synthetic peptide

<400> 30
Ser Gln Leu Glu Arg Asn Ile Glu Thr Ile 
1               5                   10  

<210> 31
<211> 16
<212> PRT
<213> Artificial Sequence


<220> 
<223> Synthetic peptide

<400> 31
Lys Leu Gly His Pro Asp Thr Leu Asn Gln Gly Glu Phe Lys Glu Leu 
1               5                   10                  15      



<210> 32
<211> 24
<212> PRT
<213> Artificial Sequence


<220> 
<223> Synthetic peptide

<400> 32
His Glu Lys Met His Glu Gly Asp Glu Gly Pro Gly His His His Lys 
1               5                   10                  15      
Pro Gly Leu Gly Glu Gly Thr Pro 
            20                  

<210> 33
<211> 27
<212> PRT
<213> Artificial Sequence


<220> 
<223> Synthetic peptide

<400> 33
Trp Ala Ser His Glu Lys Met His Glu Gly Asp Glu Gly Pro Gly His 
1               5                   10                  15      
His His Lys Pro Gly Leu Gly Glu Gly Thr Pro 
            20                  25          

<210> 34
<211> 20
<212> PRT
<213> Artificial Sequence


<220> 
<223> Synthetic peptide

<400> 34
His Glu Gly Asp Glu Gly Pro Gly His His His Lys Pro Gly Leu Gly 
1               5                   10                  15      
Glu Gly Thr Pro 
            20  

<210> 35
<211> 13
<212> PRT
<213> Artificial Sequence


<220> 
<223> Synthetic peptide

<400> 35
Met Glu Asp Leu Asp Thr Asn Ala Asp Lys Gln Leu Ser 
1               5                   10              

<210> 36
<211> 19
<212> PRT
<213> Artificial Sequence


<220> 
<223> Synthetic peptide

<400> 36
Ser His Glu Lys Met His Glu Gly Asp Glu Gly Pro Gly His His His 
1               5                   10                  15      
Lys Pro Gly 
            

<210> 37
<211> 10
<212> PRT
<213> Artificial Sequence


<220> 
<223> Synthetic peptide

<400> 37
Ile Asp Val Tyr His Lys Tyr Ser Leu Ile 
1               5                   10  

<210> 38
<211> 114
<212> PRT
<213> Homo sapiens


<220> 
<223> S100A9 Sequence

<400> 38
Met Thr Cys Lys Met Ser Gln Leu Glu Arg Asn Ile Glu Thr Ile Ile 
1               5                   10                  15      
Asn Thr Phe His Gln Tyr Ser Val Lys Leu Gly His Pro Asp Thr Leu 
            20                  25                  30          
Asn Gln Gly Glu Phe Lys Glu Leu Val Arg Lys Asp Leu Gln Asn Phe 
        35                  40                  45              
Leu Lys Lys Glu Asn Lys Asn Glu Lys Val Ile Glu His Ile Met Glu 
    50                  55                  60                  
Asp Leu Asp Thr Asn Ala Asp Lys Gln Leu Ser Phe Glu Glu Phe Ile 
65                  70                  75                  80  
Met Leu Met Ala Arg Leu Thr Trp Ala Ser His Glu Lys Met His Glu 
                85                  90                  95      
Gly Asp Glu Gly Pro Gly His His His Lys Pro Gly Leu Gly Glu Gly 
            100                 105                 110         
Thr Pro 
        

<210> 39
<211> 5
<212> PRT
<213> Artificial Sequence


<220> 
<223> S100A9 fragment

<400> 39
Met Thr Cys Lys Met 
1               5   

<210> 40
<211> 9
<212> PRT
<213> Artificial Sequence


<220> 
<223> S100A9 fragment

<400> 40
Ile Asn Thr Phe His Gln Tyr Ser Val 
1               5                   

<210> 41
<211> 12
<212> PRT
<213> Artificial Sequence


<220> 
<223> S100A9 Fragment

<400> 41
Phe Glu Glu Phe Ile Met Leu Met Ala Arg Leu Thr 
1               5                   10          

<210> 42
<211> 18
<212> PRT
<213> Artificial Sequence


<220> 
<223> S100A9 fragment

<400> 42
Ser His Glu Lys Met His Glu Gly Asp Glu Gly Pro Gly His His His 
1               5                   10                  15      
Lys Pro 
        

<210> 43
<211> 7
<212> PRT
<213> Artificial Sequence


<220> 
<223> S100A9 fragment

<400> 43
Gly Leu Gly Glu Gly Thr Pro 
1               5           

<210> 44
<211> 93
<212> PRT
<213> Homo sapiens


<220> 
<223> S100A8 Sequence

<400> 44
Met Leu Thr Glu Leu Glu Lys Ala Leu Asn Ser Ile Ile Asp Val Tyr 
1               5                   10                  15      
His Lys Tyr Ser Leu Ile Lys Gly Asn Phe His Ala Val Tyr Arg Asp 
            20                  25                  30          
Asp Leu Lys Lys Leu Leu Glu Thr Glu Cys Pro Gln Tyr Ile Arg Lys 
        35                  40                  45              
Lys Gly Ala Asp Val Trp Phe Lys Glu Leu Asp Ile Asn Thr Asp Gly 
    50                  55                  60                  
Ala Val Asn Phe Gln Glu Phe Leu Ile Leu Val Ile Lys Met Gly Val 
65                  70                  75                  80  
Ala Ala His Lys Lys Ser His Glu Glu Ser His Lys Glu 
                85                  90              

<210> 45
<211> 12
<212> PRT
<213> Artificial Sequence


<220> 
<223> S100A8 fragment

<400> 45
Met Leu Thr Glu Leu Glu Lys Ala Leu Asn Ser Ile 
1               5                   10          

<210> 46
<211> 7
<212> PRT
<213> Artificial Sequence


<220> 
<223> S100A8 fragment

<400> 46
Lys Gly Asn Phe His Ala Val 
1               5           

<210> 47
<211> 13
<212> PRT
<213> Artificial Sequence


<220> 
<223> S100A8 fragment

<400> 47
Glu Cys Pro Gln Tyr Ile Arg Lys Lys Gly Ala Asp Val 
1               5                   10              

<210> 48
<211> 27
<212> PRT
<213> Artificial Sequence


<220> 
<223> S100A8 fragment

<400> 48
Asn Phe Gln Glu Phe Leu Ile Leu Val Ile Lys Met Gly Val Ala Ala 
1               5                   10                  15      
His Lys Lys Ser His Glu Glu Ser His Lys Glu 
            20                  25          

