                         SEQUENCE LISTING

<110>  Punyani Labs AB
 
<120>  Active and orthogonal hybrid pyrrolysyl tRNA

<130>  P5572PC00

<160>  30    

<170>  PatentIn version 3.5

<210>  1
<211>  71
<212>  RNA
<213>  Artificial sequence

<220>
<223>  Hybrid PylT G1-Mx1201


<220>
<221>  tRNA
<222>  (1)..(71)
<223>  Hybrid PylT G1-Mx1201

<400>  1
gggggacggu ccggcgagca aacgggucuc uaaaaccugu aagcgggguu cgaccccccg       60

gucucucgcc a                                                            71


<210>  2
<211>  72
<212>  RNA
<213>  Artificial sequence

<220>
<223>  Mutated hybrid PylT G1-Mx1201


<220>
<221>  misc_feature
<222>  (1)..(72)
<223>  Mutated hybrid PylT G1-Mx1201

<400>  2
gggggacggu ccggcgagca aacgggucuc uaaaaccugu aaagcggggu ucgacacccc       60

ggucucucgc ca                                                           72


<210>  3
<211>  10
<212>  RNA
<213>  Artificial sequence

<220>
<223>  Acceptor stem A of M. horonobensis HB1; M. zhilinae DSM 4017; M. 
       halophilus Z7982; M. vacuolata Z761; Me. barkeri 3; M. mazei 
       WWM610; M. siciliae T4; M. thermophila TM1; M. evestigatum Z7303;
       M. portucalensis FDF1T; M. hollandica DSM 5219


<220>
<221>  misc_feature
<222>  (1)..(7)
<223>  Acceptor stem A of M. horonobensis HB1; M. zhilinae DSM 4017; M. 
       halophilus Z7982; M. vacuolata Z761; Me. barkeri 3; M. mazei 
       WWM610; M. siciliae T4; M. thermophila TM1; M. evestigatum Z7303;
       M. portucalensis FDF1T; M. hollandica DSM 5219

<400>  3
ggaaacc                                                              7


<210>  4
<211>  10
<212>  RNA
<213>  Artificial sequence

<220>
<223>  Acceptor stem A of Methanosarcina barkeri MS; Methanosarcina 
       barkeri CM1


<220>
<221>  misc_feature
<222>  (1)..(7)
<223>  Methanosarcina barkeri MS; Methanosarcina barkeri CM1

<220>
<221>  misc_feature
<222>  (1)..(7)
<223>  Acceptor stem A of Methanosarcina barkeri MS; Methanosarcina 
       barkeri CM1

<400>  4
gggaacc                                                              7


<210>  5
<211>  10
<212>  RNA
<213>  Artificial sequence

<220>
<223>  Acceptor stem A of Candidatus Methanomethylophilus alvus Mx1201; 
       Thermoplasmatales archaeon BRNA1


<220>
<221>  misc_feature
<222>  (1)..(7)
<223>  Acceptor stem A of Candidatus Methanomethylophilus alvus Mx1201; 
       Thermoplasmatales archaeon BRNA1

<400>  5
gggggac                                                              7


<210>  6
<211>  10
<212>  RNA
<213>  Artificial sequence

<220>
<223>  Acceptor stem A of Methanogenic archaeon ISO4H5 chromosome


<220>
<221>  misc_feature
<222>  (1)..(7)
<223>  Acceptor stem A of Methanogenic archaeon ISO4H5 chromosome

<400>  6
ggggggc                                                              7


<210>  7
<211>  10
<212>  RNA
<213>  Artificial sequence

<220>
<223>  Acceptor stem A of Candidatus Methanoplasma termitum MpT1


<220>
<221>  misc_feature
<222>  (1)..(7)
<223>  Acceptor stem A of Candidatus Methanoplasma termitum MpT1

<400>  7
gggagac                                                              7


<210>  8
<211>  10
<212>  RNA
<213>  Artificial sequence

<220>
<223>  Acceptor stem A of Methanosarcina lacustris Z7289; Methanolobus 
       psychrophilus R15


<220>
<221>  misc_feature
<222>  (1)..(7)
<223>  Acceptor stem A of Methanosarcina lacustris Z7289; Methanolobus 
       psychrophilus R15

<400>  8
ggaaauc                                                              7


<210>  9
<211>  10
<212>  RNA
<213>  Artificial sequence

<220>
<223>  Acceptor stem A of Methanococcoides methylutens MM1; 
       Methanococcoides burtonii DSM 6242


<220>
<221>  misc_feature
<222>  (1)..(7)
<223>  Acceptor stem A of Methanococcoides methylutens MM1; 
       Methanococcoides burtonii DSM 6242

<400>  9
ggagacu                                                              7


<210>  10
<211>  10
<212>  RNA
<213>  Artificial sequence

<220>
<223>  Acceptor stem A of Desulfitobacterium dehalogenans; 
       Desulfosporosinus orientis DSM 765; Thermacetogenium phaeum; 
       Desulfosporosinus meridiei; Desulfitobacterium hafniense; 
       Dehalobacterium formicoaceticum


<220>
<221>  misc_feature
<222>  (1)..(7)
<223>  Acceptor stem A of Desulfitobacterium dehalogenans; 
       Desulfosporosinus orientis DSM 765; Thermacetogenium phaeum; 
       Desulfosporosinus meridiei; Desulfitobacterium hafniense; 
       Dehalobacterium formicoaceticum

<400>  10
ggggggu                                                              7


<210>  11
<211>  10
<212>  RNA
<213>  Artificial sequence

<220>
<223>  Acceptor stem A of Eubacterium limosum


<220>
<221>  misc_feature
<222>  (1)..(7)
<223>  Acceptor stem A of Eubacterium limosum

<400>  11
ggagggu                                                              7


<210>  12
<211>  10
<212>  RNA
<213>  Artificial sequence

<220>
<223>  Acceptor stem A of Methanogenic archaeon ISO4-G1


<220>
<221>  misc_feature
<222>  (1)..(7)
<223>  Acceptor stem A of Methanogenic archaeon ISO4-G1

<400>  12
ggagggc                                                              7


<210>  13
<211>  11
<212>  RNA
<213>  Artificial sequence

<220>
<223>  Acceptor stem C of M. horonobensis HB1; M. zhilinae DSM 4017; M. 
       barkeri MS; M. halophilus Z7982; M. vacuolata Z761; M. barkeri 3;
       M. mazei WWM610; M. siciliae T4; M. thermophila TM1; M. lacustris
       Z7289; M. barkeri CM1; M. evestigatum Z7303; M. portucalensis


<220>
<221>  misc_feature
<222>  (1)..(11)
<223>  Acceptor stem C of M. horonobensis HB1; M. zhilinae DSM 4017; M. 
       barkeri MS; M. halophilus Z7982; M. vacuolata Z761; M. barkeri 3;
       M. mazei WWM610; M. siciliae T4; M. thermophila TM1; M. lacustris
       Z7289; M. barkeri CM1; M. evestigatum Z7303; M. portucalensis

<400>  13
gguuuccgcc a                                                            11


<210>  14
<211>  11
<212>  RNA
<213>  Artificial sequence

<220>
<223>  Acceptor stem C of Candidatus Methanomethylophilus alvus Mx1201; 
       Thermoplasmatales archaeon BRNA1; Candidatus Methanoplasma 
       termitum MpT1


<220>
<221>  misc_feature
<222>  (1)..(11)
<223>  Acceptor stem C of Candidatus Methanomethylophilus alvus Mx1201; 
       Thermoplasmatales archaeon BRNA1; Candidatus Methanoplasma 
       termitum MpT1

<400>  14
gucucucgcc a                                                            11


<210>  15
<211>  11
<212>  RNA
<213>  Artificial sequence

<220>
<223>  Acceptor stem C of Methanogenic archaeon ISO4H5 chromosome


<220>
<221>  misc_feature
<222>  (1)..(11)
<223>  Acceptor stem C of Methanogenic archaeon ISO4H5 chromosome

<400>  15
gccucucgcc a                                                            11


<210>  16
<211>  11
<212>  RNA
<213>  Artificial sequence

<220>
<223>  Acceptor stem C of Methanococcoides methylutens MM1; 
       Methanococcoides burtonii DSM 6242


<220>
<221>  misc_feature
<222>  (1)..(11)
<223>  Acceptor stem C of Methanococcoides methylutens MM1; 
       Methanococcoides burtonii DSM 6242

<400>  16
aguuuccgcc a                                                            11


<210>  17
<211>  11
<212>  RNA
<213>  Artificial sequence

<220>
<223>  Acceptor stem C of Desulfitobacterium dehalogenans; 
       Desulfosporosinus orientis DSM 765; Desulfosporosinus meridiei; 
       Thermacetogenium phaeum; Desulfitobacterium hafniense; 
       Dehalobacterium formicoaceticum


<220>
<221>  misc_feature
<222>  (1)..(11)
<223>  Acceptor stem C of Desulfitobacterium dehalogenans; 
       Desulfosporosinus orientis DSM 765; Desulfosporosinus meridiei; 
       Thermacetogenium phaeum; Desulfitobacterium hafniense; 
       Dehalobacterium formicoaceticum

<400>  17
acuccccgcc a                                                            11


<210>  18
<211>  11
<212>  RNA
<213>  Artificial sequence

<220>
<223>  Acceptor stem C of Eubacterium limosum


<220>
<221>  misc_feature
<222>  (1)..(11)
<223>  Acceptor stem C of Eubacterium limosum

<400>  18
acucuccacc a                                                            11


<210>  19
<211>  11
<212>  RNA
<213>  Artificial sequence

<220>
<223>  Acceptor stem C of Methanogenic archaeon ISO4-G1


<220>
<221>  misc_feature
<222>  (1)..(11)
<223>  Acceptor stem C of Methanogenic archaeon ISO4-G1

<400>  19
gccuuucgcc a                                                            11


<210>  20
<211>  275
<212>  PRT
<213>  Candidatus Methanomethylophilus alvus Mx1201

<400>  20

Met Thr Val Lys Tyr Thr Asp Ala Gln Ile Gln Arg Leu Arg Glu Tyr 
1               5                   10                  15      


Gly Asn Gly Thr Tyr Glu Gln Lys Val Phe Glu Asp Leu Ala Ser Arg 
            20                  25                  30          


Asp Ala Ala Phe Ser Lys Glu Met Ser Val Ala Ser Thr Asp Asn Glu 
        35                  40                  45              


Lys Lys Ile Lys Gly Met Ile Ala Asn Pro Ser Arg His Gly Leu Thr 
    50                  55                  60                  


Gln Leu Met Asn Asp Ile Ala Asp Ala Leu Val Ala Glu Gly Phe Ile 
65                  70                  75                  80  


Glu Val Arg Thr Pro Ile Phe Ile Ser Lys Asp Ala Leu Ala Arg Met 
                85                  90                  95      


Thr Ile Thr Glu Asp Lys Pro Leu Phe Lys Gln Val Phe Trp Ile Asp 
            100                 105                 110         


Glu Lys Arg Ala Leu Arg Pro Met Leu Ala Pro Asn Leu Tyr Ser Val 
        115                 120                 125             


Met Arg Asp Leu Arg Asp His Thr Asp Gly Pro Val Lys Ile Phe Glu 
    130                 135                 140                 


Met Gly Ser Cys Phe Arg Lys Glu Ser His Ser Gly Met His Leu Glu 
145                 150                 155                 160 


Glu Phe Thr Met Leu Asn Leu Val Asp Met Gly Pro Arg Gly Asp Ala 
                165                 170                 175     


Thr Glu Val Leu Lys Asn Tyr Ile Ser Val Val Met Lys Ala Ala Gly 
            180                 185                 190         


Leu Pro Asp Tyr Asp Leu Val Gln Glu Glu Ser Asp Val Tyr Lys Glu 
        195                 200                 205             


Thr Ile Asp Val Glu Ile Asn Gly Gln Glu Val Cys Ser Ala Ala Val 
    210                 215                 220                 


Gly Pro His Tyr Leu Asp Ala Ala His Asp Val His Glu Pro Trp Ser 
225                 230                 235                 240 


Gly Ala Gly Phe Gly Leu Glu Arg Leu Leu Thr Ile Arg Glu Lys Tyr 
                245                 250                 255     


Ser Thr Val Lys Lys Gly Gly Ala Ser Ile Ser Tyr Leu Asn Gly Ala 
            260                 265                 270         


Lys Ile Asn 
        275 


<210>  21
<211>  828
<212>  DNA
<213>  Candidatus Methanomethylophilus alvus Mx1201

<400>  21
atgacggtaa agtatacgga tgcacagata cagcgcctca gggaatacgg caacgggacc       60

tatgagcaga aggtcttcga ggacctcgca tcgagggatg ctgccttctc caaggagatg      120

tccgtcgcct ctaccgacaa cgagaagaag atcaagggga tgatcgccaa tccgtcccgt      180

catggattga cccagctgat gaacgacatc gccgacgcat tggtcgccga gggtttcatc      240

gaggtccgta cgcccatatt catatcgaag gatgcgctgg cacgtatgac catcaccgag      300

gacaagcccc ttttcaagca ggtcttctgg atcgacgaga aaagggcgct caggcctatg      360

ctggcaccta acctttattc cgtcatgagg gacctgaggg accatacgga cggtccggtg      420

aagatcttcg agatgggttc ctgcttcagg aaggagtccc acagcgggat gcatctggag      480

gagttcacca tgctgaacct cgtggacatg ggtccccgcg gagacgccac ggaggtcctg      540

aagaactaca tatcggtcgt gatgaaggcg gccggtctcc cggactacga cctcgtacag      600

gaggagtccg acgtatacaa ggagaccata gacgtggaga tcaacggtca ggaggtctgt      660

tccgcagccg tcggtccaca ctatctcgat gcggcccacg atgtccacga gccttggtcc      720

ggagcgggat tcggtctcga acgcctgctg accatcaggg agaagtacag caccgtgaag      780

aagggaggag ccagcatcag ctacctcaac ggtgcgaaga tcaactga                   828


<210>  22
<211>  273
<212>  PRT
<213>  Methanogenic archaeon ISO4-G1

<400>  22

Met Val Val Lys Phe Thr Asp Ser Gln Ile Gln His Leu Met Glu Tyr 
1               5                   10                  15      


Gly Asp Asn Asp Trp Ser Glu Ala Glu Phe Glu Asp Ala Ala Ala Arg 
            20                  25                  30          


Asp Lys Glu Phe Ser Ser Gln Phe Ser Lys Leu Lys Ser Ala Asn Asp 
        35                  40                  45              


Lys Gly Leu Lys Asp Val Ile Ala Asn Pro Arg Asn Asp Leu Thr Asp 
    50                  55                  60                  


Leu Glu Asn Lys Ile Arg Glu Lys Leu Ala Ala Arg Gly Phe Ile Glu 
65                  70                  75                  80  


Val His Thr Pro Ile Phe Val Ser Lys Ser Ala Leu Ala Lys Met Thr 
                85                  90                  95      


Ile Thr Glu Asp His Pro Leu Phe Lys Gln Val Phe Trp Ile Asp Asp 
            100                 105                 110         


Lys Arg Ala Leu Arg Pro Met His Ala Met Asn Leu Tyr Lys Val Met 
        115                 120                 125             


Arg Glu Leu Arg Asp His Thr Lys Gly Pro Val Lys Ile Phe Glu Ile 
    130                 135                 140                 


Gly Ser Cys Phe Arg Lys Glu Ser Lys Ser Ser Thr His Leu Glu Glu 
145                 150                 155                 160 


Phe Thr Met Leu Asn Leu Val Glu Met Gly Pro Asp Gly Asp Pro Met 
                165                 170                 175     


Glu His Leu Lys Met Tyr Ile Gly Asp Ile Met Asp Ala Val Gly Val 
            180                 185                 190         


Glu Tyr Thr Thr Ser Arg Glu Glu Ser Asp Val Tyr Val Glu Thr Leu 
        195                 200                 205             


Asp Val Glu Ile Asn Gly Thr Glu Val Ala Ser Gly Ala Val Gly Pro 
    210                 215                 220                 


His Lys Leu Asp Pro Ala His Asp Val His Glu Pro Trp Ala Gly Ile 
225                 230                 235                 240 


Gly Phe Gly Leu Glu Arg Leu Leu Met Leu Lys Asn Gly Lys Ser Asn 
                245                 250                 255     


Ala Arg Lys Thr Gly Lys Ser Ile Thr Tyr Leu Asn Gly Tyr Lys Leu 
            260                 265                 270         


Asp 
    


<210>  23
<211>  822
<212>  DNA
<213>  Methanogenic archaeon ISO4-G1

<400>  23
atggttgtca agttcaccga ttcccagatc cagcacctaa tggagtatgg ggacaatgat       60

tggtcggagg cagagttcga ggatgccgcc gccagggata aggaattctc atcacagttc      120

tccaaactga aatccgccaa cgataagggc ctcaaggatg tcatcgccaa cccgcgcaac      180

gacctcaccg acctggagaa caagatcagg gagaagcttg cagccagggg tttcattgaa      240

gttcacacgc ccatattcgt ttcaaagagc gccctcgcca agatgacgat caccgaggac      300

cacccgctgt tcaagcaggt gttctggatc gacgacaagc gtgcgctcag gcccatgcac      360

gcaatgaacc tgtacaaggt catgagggag ctcagggacc acacgaaggg tccggtcaag      420

atcttcgaga tcggctcctg cttcaggaag gagtccaaga gctccaccca cctcgaggag      480

ttcacgatgc tgaacctggt ggagatgggc cccgacgggg atccgatgga gcatctgaag      540

atgtacatcg gggacatcat ggacgccgtc ggcgtcgaat ataccacatc ccgcgaggag      600

tccgacgtgt acgtcgagac cctcgacgtg gagatcaacg gtacggaggt cgcttccgga      660

gcggtcggac ctcacaagct ggatccggca cacgacgtcc acgagccgtg ggcagggatc      720

ggattcggac tggagcgcct gctcatgctg aagaacggca aaagcaacgc caggaagaca      780

ggcaagagca tcacgtatct gaacggatac aaactggact ga                         822


<210>  24
<211>  454
<212>  PRT
<213>  Methanosarcina mazei

<400>  24

Met Asp Lys Lys Pro Leu Asn Thr Leu Ile Ser Ala Thr Gly Leu Trp 
1               5                   10                  15      


Met Ser Arg Thr Gly Thr Ile His Lys Ile Lys His His Glu Val Ser 
            20                  25                  30          


Arg Ser Lys Ile Tyr Ile Glu Met Ala Cys Gly Asp His Leu Val Val 
        35                  40                  45              


Asn Asn Ser Arg Ser Ser Arg Thr Ala Arg Ala Leu Arg His His Lys 
    50                  55                  60                  


Tyr Arg Lys Thr Cys Lys Arg Cys Arg Val Ser Asp Glu Asp Leu Asn 
65                  70                  75                  80  


Lys Phe Leu Thr Lys Ala Asn Glu Asp Gln Thr Ser Val Lys Val Lys 
                85                  90                  95      


Val Val Ser Ala Pro Thr Arg Thr Lys Lys Ala Met Pro Lys Ser Val 
            100                 105                 110         


Ala Arg Ala Pro Lys Pro Leu Glu Asn Thr Glu Ala Ala Gln Ala Gln 
        115                 120                 125             


Pro Ser Gly Ser Lys Phe Ser Pro Ala Ile Pro Val Ser Thr Gln Glu 
    130                 135                 140                 


Ser Val Ser Val Pro Ala Ser Val Ser Thr Ser Ile Ser Ser Ile Ser 
145                 150                 155                 160 


Thr Gly Ala Thr Ala Ser Ala Leu Val Lys Gly Asn Thr Asn Pro Ile 
                165                 170                 175     


Thr Ser Met Ser Ala Pro Val Gln Ala Ser Ala Pro Ala Leu Thr Lys 
            180                 185                 190         


Ser Gln Thr Asp Arg Leu Glu Val Leu Leu Asn Pro Lys Asp Glu Ile 
        195                 200                 205             


Ser Leu Asn Ser Gly Lys Pro Phe Arg Glu Leu Glu Ser Glu Leu Leu 
    210                 215                 220                 


Ser Arg Arg Lys Lys Asp Leu Gln Gln Ile Tyr Ala Glu Glu Arg Glu 
225                 230                 235                 240 


Asn Tyr Leu Gly Lys Leu Glu Arg Glu Ile Thr Arg Phe Phe Val Asp 
                245                 250                 255     


Arg Gly Phe Leu Glu Ile Lys Ser Pro Ile Leu Ile Pro Leu Glu Tyr 
            260                 265                 270         


Ile Glu Arg Met Gly Ile Asp Asn Asp Thr Glu Leu Ser Lys Gln Ile 
        275                 280                 285             


Phe Arg Val Asp Lys Asn Phe Cys Leu Arg Pro Met Leu Ala Pro Asn 
    290                 295                 300                 


Leu Tyr Asn Tyr Leu Arg Lys Leu Asp Arg Ala Leu Pro Asp Pro Ile 
305                 310                 315                 320 


Lys Ile Phe Glu Ile Gly Pro Cys Tyr Arg Lys Glu Ser Asp Gly Lys 
                325                 330                 335     


Glu His Leu Glu Glu Phe Thr Met Leu Asn Phe Cys Gln Met Gly Ser 
            340                 345                 350         


Gly Cys Thr Arg Glu Asn Leu Glu Ser Ile Ile Thr Asp Phe Leu Asn 
        355                 360                 365             


His Leu Gly Ile Asp Phe Lys Ile Val Gly Asp Ser Cys Met Val Tyr 
    370                 375                 380                 


Gly Asp Thr Leu Asp Val Met His Gly Asp Leu Glu Leu Ser Ser Ala 
385                 390                 395                 400 


Val Val Gly Pro Ile Pro Leu Asp Arg Glu Trp Gly Ile Asp Lys Pro 
                405                 410                 415     


Trp Ile Gly Ala Gly Phe Gly Leu Glu Arg Leu Leu Lys Val Lys His 
            420                 425                 430         


Asp Phe Lys Asn Ile Lys Arg Ala Ala Arg Ser Glu Ser Tyr Tyr Asn 
        435                 440                 445             


Gly Ile Ser Thr Asn Leu 
    450                 


<210>  25
<211>  1365
<212>  DNA
<213>  Methanosarcina mazei

<400>  25
atggataaaa aaccactaaa cactctgata tctgcaaccg ggctctggat gtccaggacc       60

ggaacaattc ataaaataaa acaccacgaa gtctctcgaa gcaaaatcta tattgaaatg      120

gcatgcggag accaccttgt tgtaaacaac tccaggagca gcaggactgc aagagcgctc      180

aggcaccaca aatacaggaa gacctgcaaa cgctgcaggg tttcggatga ggatctcaat      240

aagttcctca caaaggcaaa cgaagaccag acaagcgtaa aagtcaaggt cgtttctgcc      300

cctaccagaa cgaaaaaggc aatgccaaaa tccgttgcga gagccccgaa acctcttgag      360

aatacagaag cggcacaggc tcaaccttct ggatctaaat tttcacctgc gataccggtt      420

tccacccaag agtcagtttc tgtcccggca tctgtttcaa catcaatatc aagcatttct      480

acaggagcaa ctgcatccgc actggtaaaa gggaatacga accccattac atccatgtct      540

gcccctgttc aggcaagtgc ccccgcactt acgaagagcc agactgacag gcttgaagtc      600

ctgttaaacc caaaagatga gatttccctg aattccggca agcctttcag ggagcttgag      660

tccgaattgc tctctcgcag aaaaaaagac ctgcagcaga tctacgcgga agaaagggag      720

aattatctgg ggaaactcga gcgtgaaatt accaggttct ttgtggacag gggttttctg      780

gaaataaaat ccccgatcct gatccctctt gagtatatcg aaaggatggg cattgataat      840

gataccgaac tttcaaaaca gatcttcagg gttgacaaga acttctgcct gagacccatg      900

cttgctccaa acctttacaa ctacctgcgc aagcttgaca gggccctgcc tgatccaata      960

aaaatttttg aaataggccc atgctacaga aaagagtccg acggcaaaga acacctcgaa     1020

gagtttacca tgctgaactt ctgccagatg ggatcgggat gcacacggga aaatcttgaa     1080

agcataatta cagacttcct gaaccacctg ggaattgatt tcaagatcgt aggcgattcc     1140

tgcatggtct atggggatac ccttgatgta atgcacggag acctggaact ttcctctgca     1200

gtagtcggac ccataccgct tgaccgggaa tggggtattg ataaaccctg gataggggca     1260

ggtttcgggc tcgaacgcct tctcaaggtt aaacacgact ttaaaaatat caagagagct     1320

gcaaggtccg agtcttacta taacgggatt tctaccaacc tgtaa                     1365


<210>  26
<211>  273
<212>  PRT
<213>  Methanogenium archaeon ISO4-G1 Y125A mutant

<400>  26

Met Val Val Lys Phe Thr Asp Ser Gln Ile Gln His Leu Met Glu Tyr 
1               5                   10                  15      


Gly Asp Asn Asp Trp Ser Glu Ala Glu Phe Glu Asp Ala Ala Ala Arg 
            20                  25                  30          


Asp Lys Glu Phe Ser Ser Gln Phe Ser Lys Leu Lys Ser Ala Asn Asp 
        35                  40                  45              


Lys Gly Leu Lys Asp Val Ile Ala Asn Pro Arg Asn Asp Leu Thr Asp 
    50                  55                  60                  


Leu Glu Asn Lys Ile Arg Glu Lys Leu Ala Ala Arg Gly Phe Ile Glu 
65                  70                  75                  80  


Val His Thr Pro Ile Phe Val Ser Lys Ser Ala Leu Ala Lys Met Thr 
                85                  90                  95      


Ile Thr Glu Asp His Pro Leu Phe Lys Gln Val Phe Trp Ile Asp Asp 
            100                 105                 110         


Lys Arg Ala Leu Arg Pro Met His Ala Met Asn Leu Ala Lys Val Met 
        115                 120                 125             


Arg Glu Leu Arg Asp His Thr Lys Gly Pro Val Lys Ile Phe Glu Ile 
    130                 135                 140                 


Gly Ser Cys Phe Arg Lys Glu Ser Lys Ser Ser Thr His Leu Glu Glu 
145                 150                 155                 160 


Phe Thr Met Leu Asn Leu Val Glu Met Gly Pro Asp Gly Asp Pro Met 
                165                 170                 175     


Glu His Leu Lys Met Tyr Ile Gly Asp Ile Met Asp Ala Val Gly Val 
            180                 185                 190         


Glu Tyr Thr Thr Ser Arg Glu Glu Ser Asp Val Tyr Val Glu Thr Leu 
        195                 200                 205             


Asp Val Glu Ile Asn Gly Thr Glu Val Ala Ser Gly Ala Val Gly Pro 
    210                 215                 220                 


His Lys Leu Asp Pro Ala His Asp Val His Glu Pro Trp Ala Gly Ile 
225                 230                 235                 240 


Gly Phe Gly Leu Glu Arg Leu Leu Met Leu Lys Asn Gly Lys Ser Asn 
                245                 250                 255     


Ala Arg Lys Thr Gly Lys Ser Ile Thr Tyr Leu Asn Gly Tyr Lys Leu 
            260                 265                 270         


Asp 
    


<210>  27
<211>  822
<212>  DNA
<213>  Methanogenium archaeon ISO4- G1, Y125A mutant

<400>  27
atggttgtca agttcaccga ttcccagatc cagcacctaa tggagtatgg ggacaatgat       60

tggtcggagg cagagttcga ggatgccgcc gccagggata aggaattctc atcacagttc      120

tccaaactga aatccgccaa cgataagggc ctcaaggatg tcatcgccaa cccgcgcaac      180

gacctcaccg acctggagaa caagatcagg gagaagcttg cagccagggg tttcattgaa      240

gttcacacgc ccatattcgt ttcaaagagc gccctcgcca agatgacgat caccgaggac      300

cacccgctgt tcaagcaggt gttctggatc gacgacaagc gtgcgctcag gcccatgcac      360

gcaatgaacc tggccaaggt catgagggag ctcagggacc acacgaaggg tccggtcaag      420

atcttcgaga tcggctcctg cttcaggaag gagtccaaga gctccaccca cctcgaggag      480

ttcacgatgc tgaacctggt ggagatgggc cccgacgggg atccgatgga gcatctgaag      540

atgtacatcg gggacatcat ggacgccgtc ggcgtcgaat ataccacatc ccgcgaggag      600

tccgacgtgt acgtcgagac cctcgacgtg gagatcaacg gtacggaggt cgcttccgga      660

gcggtcggac ctcacaagct ggatccggca cacgacgtcc acgagccgtg ggcagggatc      720

ggattcggac tggagcgcct gctcatgctg aagaacggca aaagcaacgc caggaagaca      780

ggcaagagca tcacgtatct gaacggatac aaactggact ga                         822


<210>  28
<211>  71
<212>  RNA
<213>  Artificial sequence

<220>
<223>  Hybrid PylT G1-ISO-H5


<220>
<221>  tRNA
<222>  (1)..(71)
<223>  Hybrid PylT G1-ISO-H5

<400>  28
ggggggcgcu ccggcgagca aacgggucuc uaaaaccugu aagcgggguu cgaccccccg       60

gccucucgcc a                                                            71


<210>  29
<211>  71
<212>  RNA
<213>  Artificial sequence

<220>
<223>  Hybrid PylT G1-MpT1


<220>
<221>  tRNA
<222>  (1)..(71)
<223>  Hybrid PylT G1-MpT1

<400>  29
ggggggcgcu ccggcgagca aacgggucuc uaaaaccugu aagcgggguu cgaccccccg       60

gccucucgcc a                                                            71


<210>  30
<211>  72
<212>  RNA
<213>  Methanosarcina mazeii

<400>  30
ggaaaccuga ucauguagau cgaacggacu cuaaauccgu ucagccgggu uagauucccg       60

ggguuuccgc ca                                                           72


