                         SEQUENCE LISTING

<110>  Lysando AG
 
<120>  Virus neutralization by soluble receptor fragments of the ACE-2 
       receptor

<130>  LYS-054 PCT

<140>  unknown
<141>  2021-05-11

<150>  EP20173886.1
<151>  2020-05-11

<160>  43    

<170>  PatentIn version 3.5

<210>  1
<211>  805
<212>  PRT
<213>  Homo sapiens Full-length ACE-2

<400>  1

Met Ser Ser Ser Ser Trp Leu Leu Leu Ser Leu Val Ala Val Thr Ala 
1               5                   10                  15      


Ala Gln Ser Thr Ile Glu Glu Gln Ala Lys Thr Phe Leu Asp Lys Phe 
            20                  25                  30          


Asn His Glu Ala Glu Asp Leu Phe Tyr Gln Ser Ser Leu Ala Ser Trp 
        35                  40                  45              


Asn Tyr Asn Thr Asn Ile Thr Glu Glu Asn Val Gln Asn Met Asn Asn 
    50                  55                  60                  


Ala Gly Asp Lys Trp Ser Ala Phe Leu Lys Glu Gln Ser Thr Leu Ala 
65                  70                  75                  80  


Gln Met Tyr Pro Leu Gln Glu Ile Gln Asn Leu Thr Val Lys Leu Gln 
                85                  90                  95      


Leu Gln Ala Leu Gln Gln Asn Gly Ser Ser Val Leu Ser Glu Asp Lys 
            100                 105                 110         


Ser Lys Arg Leu Asn Thr Ile Leu Asn Thr Met Ser Thr Ile Tyr Ser 
        115                 120                 125             


Thr Gly Lys Val Cys Asn Pro Asp Asn Pro Gln Glu Cys Leu Leu Leu 
    130                 135                 140                 


Glu Pro Gly Leu Asn Glu Ile Met Ala Asn Ser Leu Asp Tyr Asn Glu 
145                 150                 155                 160 


Arg Leu Trp Ala Trp Glu Ser Trp Arg Ser Glu Val Gly Lys Gln Leu 
                165                 170                 175     


Arg Pro Leu Tyr Glu Glu Tyr Val Val Leu Lys Asn Glu Met Ala Arg 
            180                 185                 190         


Ala Asn His Tyr Glu Asp Tyr Gly Asp Tyr Trp Arg Gly Asp Tyr Glu 
        195                 200                 205             


Val Asn Gly Val Asp Gly Tyr Asp Tyr Ser Arg Gly Gln Leu Ile Glu 
    210                 215                 220                 


Asp Val Glu His Thr Phe Glu Glu Ile Lys Pro Leu Tyr Glu His Leu 
225                 230                 235                 240 


His Ala Tyr Val Arg Ala Lys Leu Met Asn Ala Tyr Pro Ser Tyr Ile 
                245                 250                 255     


Ser Pro Ile Gly Cys Leu Pro Ala His Leu Leu Gly Asp Met Trp Gly 
            260                 265                 270         


Arg Phe Trp Thr Asn Leu Tyr Ser Leu Thr Val Pro Phe Gly Gln Lys 
        275                 280                 285             


Pro Asn Ile Asp Val Thr Asp Ala Met Val Asp Gln Ala Trp Asp Ala 
    290                 295                 300                 


Gln Arg Ile Phe Lys Glu Ala Glu Lys Phe Phe Val Ser Val Gly Leu 
305                 310                 315                 320 


Pro Asn Met Thr Gln Gly Phe Trp Glu Asn Ser Met Leu Thr Asp Pro 
                325                 330                 335     


Gly Asn Val Gln Lys Ala Val Cys His Pro Thr Ala Trp Asp Leu Gly 
            340                 345                 350         


Lys Gly Asp Phe Arg Ile Leu Met Cys Thr Lys Val Thr Met Asp Asp 
        355                 360                 365             


Phe Leu Thr Ala His His Glu Met Gly His Ile Gln Tyr Asp Met Ala 
    370                 375                 380                 


Tyr Ala Ala Gln Pro Phe Leu Leu Arg Asn Gly Ala Asn Glu Gly Phe 
385                 390                 395                 400 


His Glu Ala Val Gly Glu Ile Met Ser Leu Ser Ala Ala Thr Pro Lys 
                405                 410                 415     


His Leu Lys Ser Ile Gly Leu Leu Ser Pro Asp Phe Gln Glu Asp Asn 
            420                 425                 430         


Glu Thr Glu Ile Asn Phe Leu Leu Lys Gln Ala Leu Thr Ile Val Gly 
        435                 440                 445             


Thr Leu Pro Phe Thr Tyr Met Leu Glu Lys Trp Arg Trp Met Val Phe 
    450                 455                 460                 


Lys Gly Glu Ile Pro Lys Asp Gln Trp Met Lys Lys Trp Trp Glu Met 
465                 470                 475                 480 


Lys Arg Glu Ile Val Gly Val Val Glu Pro Val Pro His Asp Glu Thr 
                485                 490                 495     


Tyr Cys Asp Pro Ala Ser Leu Phe His Val Ser Asn Asp Tyr Ser Phe 
            500                 505                 510         


Ile Arg Tyr Tyr Thr Arg Thr Leu Tyr Gln Phe Gln Phe Gln Glu Ala 
        515                 520                 525             


Leu Cys Gln Ala Ala Lys His Glu Gly Pro Leu His Lys Cys Asp Ile 
    530                 535                 540                 


Ser Asn Ser Thr Glu Ala Gly Gln Lys Leu Phe Asn Met Leu Arg Leu 
545                 550                 555                 560 


Gly Lys Ser Glu Pro Trp Thr Leu Ala Leu Glu Asn Val Val Gly Ala 
                565                 570                 575     


Lys Asn Met Asn Val Arg Pro Leu Leu Asn Tyr Phe Glu Pro Leu Phe 
            580                 585                 590         


Thr Trp Leu Lys Asp Gln Asn Lys Asn Ser Phe Val Gly Trp Ser Thr 
        595                 600                 605             


Asp Trp Ser Pro Tyr Ala Asp Gln Ser Ile Lys Val Arg Ile Ser Leu 
    610                 615                 620                 


Lys Ser Ala Leu Gly Asp Lys Ala Tyr Glu Trp Asn Asp Asn Glu Met 
625                 630                 635                 640 


Tyr Leu Phe Arg Ser Ser Val Ala Tyr Ala Met Arg Gln Tyr Phe Leu 
                645                 650                 655     


Lys Val Lys Asn Gln Met Ile Leu Phe Gly Glu Glu Asp Val Arg Val 
            660                 665                 670         


Ala Asn Leu Lys Pro Arg Ile Ser Phe Asn Phe Phe Val Thr Ala Pro 
        675                 680                 685             


Lys Asn Val Ser Asp Ile Ile Pro Arg Thr Glu Val Glu Lys Ala Ile 
    690                 695                 700                 


Arg Met Ser Arg Ser Arg Ile Asn Asp Ala Phe Arg Leu Asn Asp Asn 
705                 710                 715                 720 


Ser Leu Glu Phe Leu Gly Ile Gln Pro Thr Leu Gly Pro Pro Asn Gln 
                725                 730                 735     


Pro Pro Val Ser Ile Trp Leu Ile Val Phe Gly Val Val Met Gly Val 
            740                 745                 750         


Ile Val Val Gly Ile Val Ile Leu Ile Phe Thr Gly Ile Arg Asp Arg 
        755                 760                 765             


Lys Lys Lys Asn Lys Ala Arg Ser Gly Glu Asn Pro Tyr Ala Ser Ile 
    770                 775                 780                 


Asp Ile Ser Lys Gly Glu Asn Asn Pro Gly Phe Gln Asn Thr Asp Asp 
785                 790                 795                 800 


Val Gln Thr Ser Phe 
                805 


<210>  2
<211>  112
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Neck domain (616-726) of human ACE-2

<400>  2

Gln Ser Ile Lys Val Arg Ile Ser Leu Lys Ser Ala Leu Gly Asp Lys 
1               5                   10                  15      


Ala Tyr Glu Trp Asn Asp Asn Glu Met Tyr Leu Phe Arg Ser Ser Val 
            20                  25                  30          


Ala Tyr Ala Met Arg Gln Tyr Phe Leu Lys Val Lys Asn Gln Met Ile 
        35                  40                  45              


Leu Phe Gly Glu Glu Asp Val Arg Val Ala Asn Leu Lys Pro Arg Ile 
    50                  55                  60                  


Ser Phe Asn Phe Phe Val Thr Ala Pro Lys Asn Val Ser Asp Ile Ile 
65                  70                  75                  80  


Pro Arg Thr Glu Val Glu Lys Ala Ile Arg Met Ser Arg Ser Arg Ile 
                85                  90                  95      


Asn Asp Ala Phe Arg Leu Asn Asp Asn Ser Leu Glu Phe Leu Gly Ile 
            100                 105                 110         


<210>  3
<211>  615
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Peptidase domain (1-615) of human ACE-2

<400>  3

Met Ser Ser Ser Ser Trp Leu Leu Leu Ser Leu Val Ala Val Thr Ala 
1               5                   10                  15      


Ala Gln Ser Thr Ile Glu Glu Gln Ala Lys Thr Phe Leu Asp Lys Phe 
            20                  25                  30          


Asn His Glu Ala Glu Asp Leu Phe Tyr Gln Ser Ser Leu Ala Ser Trp 
        35                  40                  45              


Asn Tyr Asn Thr Asn Ile Thr Glu Glu Asn Val Gln Asn Met Asn Asn 
    50                  55                  60                  


Ala Gly Asp Lys Trp Ser Ala Phe Leu Lys Glu Gln Ser Thr Leu Ala 
65                  70                  75                  80  


Gln Met Tyr Pro Leu Gln Glu Ile Gln Asn Leu Thr Val Lys Leu Gln 
                85                  90                  95      


Leu Gln Ala Leu Gln Gln Asn Gly Ser Ser Val Leu Ser Glu Asp Lys 
            100                 105                 110         


Ser Lys Arg Leu Asn Thr Ile Leu Asn Thr Met Ser Thr Ile Tyr Ser 
        115                 120                 125             


Thr Gly Lys Val Cys Asn Pro Asp Asn Pro Gln Glu Cys Leu Leu Leu 
    130                 135                 140                 


Glu Pro Gly Leu Asn Glu Ile Met Ala Asn Ser Leu Asp Tyr Asn Glu 
145                 150                 155                 160 


Arg Leu Trp Ala Trp Glu Ser Trp Arg Ser Glu Val Gly Lys Gln Leu 
                165                 170                 175     


Arg Pro Leu Tyr Glu Glu Tyr Val Val Leu Lys Asn Glu Met Ala Arg 
            180                 185                 190         


Ala Asn His Tyr Glu Asp Tyr Gly Asp Tyr Trp Arg Gly Asp Tyr Glu 
        195                 200                 205             


Val Asn Gly Val Asp Gly Tyr Asp Tyr Ser Arg Gly Gln Leu Ile Glu 
    210                 215                 220                 


Asp Val Glu His Thr Phe Glu Glu Ile Lys Pro Leu Tyr Glu His Leu 
225                 230                 235                 240 


His Ala Tyr Val Arg Ala Lys Leu Met Asn Ala Tyr Pro Ser Tyr Ile 
                245                 250                 255     


Ser Pro Ile Gly Cys Leu Pro Ala His Leu Leu Gly Asp Met Trp Gly 
            260                 265                 270         


Arg Phe Trp Thr Asn Leu Tyr Ser Leu Thr Val Pro Phe Gly Gln Lys 
        275                 280                 285             


Pro Asn Ile Asp Val Thr Asp Ala Met Val Asp Gln Ala Trp Asp Ala 
    290                 295                 300                 


Gln Arg Ile Phe Lys Glu Ala Glu Lys Phe Phe Val Ser Val Gly Leu 
305                 310                 315                 320 


Pro Asn Met Thr Gln Gly Phe Trp Glu Asn Ser Met Leu Thr Asp Pro 
                325                 330                 335     


Gly Asn Val Gln Lys Ala Val Cys His Pro Thr Ala Trp Asp Leu Gly 
            340                 345                 350         


Lys Gly Asp Phe Arg Ile Leu Met Cys Thr Lys Val Thr Met Asp Asp 
        355                 360                 365             


Phe Leu Thr Ala His His Glu Met Gly His Ile Gln Tyr Asp Met Ala 
    370                 375                 380                 


Tyr Ala Ala Gln Pro Phe Leu Leu Arg Asn Gly Ala Asn Glu Gly Phe 
385                 390                 395                 400 


His Glu Ala Val Gly Glu Ile Met Ser Leu Ser Ala Ala Thr Pro Lys 
                405                 410                 415     


His Leu Lys Ser Ile Gly Leu Leu Ser Pro Asp Phe Gln Glu Asp Asn 
            420                 425                 430         


Glu Thr Glu Ile Asn Phe Leu Leu Lys Gln Ala Leu Thr Ile Val Gly 
        435                 440                 445             


Thr Leu Pro Phe Thr Tyr Met Leu Glu Lys Trp Arg Trp Met Val Phe 
    450                 455                 460                 


Lys Gly Glu Ile Pro Lys Asp Gln Trp Met Lys Lys Trp Trp Glu Met 
465                 470                 475                 480 


Lys Arg Glu Ile Val Gly Val Val Glu Pro Val Pro His Asp Glu Thr 
                485                 490                 495     


Tyr Cys Asp Pro Ala Ser Leu Phe His Val Ser Asn Asp Tyr Ser Phe 
            500                 505                 510         


Ile Arg Tyr Tyr Thr Arg Thr Leu Tyr Gln Phe Gln Phe Gln Glu Ala 
        515                 520                 525             


Leu Cys Gln Ala Ala Lys His Glu Gly Pro Leu His Lys Cys Asp Ile 
    530                 535                 540                 


Ser Asn Ser Thr Glu Ala Gly Gln Lys Leu Phe Asn Met Leu Arg Leu 
545                 550                 555                 560 


Gly Lys Ser Glu Pro Trp Thr Leu Ala Leu Glu Asn Val Val Gly Ala 
                565                 570                 575     


Lys Asn Met Asn Val Arg Pro Leu Leu Asn Tyr Phe Glu Pro Leu Phe 
            580                 585                 590         


Thr Trp Leu Lys Asp Gln Asn Lys Asn Ser Phe Val Gly Trp Ser Thr 
        595                 600                 605             


Asp Trp Ser Pro Tyr Ala Asp 
    610                 615 


<210>  4
<211>  597
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Fragment of Peptidase domain (19-615) of human ACE-2

<400>  4

Ser Thr Ile Glu Glu Gln Ala Lys Thr Phe Leu Asp Lys Phe Asn His 
1               5                   10                  15      


Glu Ala Glu Asp Leu Phe Tyr Gln Ser Ser Leu Ala Ser Trp Asn Tyr 
            20                  25                  30          


Asn Thr Asn Ile Thr Glu Glu Asn Val Gln Asn Met Asn Asn Ala Gly 
        35                  40                  45              


Asp Lys Trp Ser Ala Phe Leu Lys Glu Gln Ser Thr Leu Ala Gln Met 
    50                  55                  60                  


Tyr Pro Leu Gln Glu Ile Gln Asn Leu Thr Val Lys Leu Gln Leu Gln 
65                  70                  75                  80  


Ala Leu Gln Gln Asn Gly Ser Ser Val Leu Ser Glu Asp Lys Ser Lys 
                85                  90                  95      


Arg Leu Asn Thr Ile Leu Asn Thr Met Ser Thr Ile Tyr Ser Thr Gly 
            100                 105                 110         


Lys Val Cys Asn Pro Asp Asn Pro Gln Glu Cys Leu Leu Leu Glu Pro 
        115                 120                 125             


Gly Leu Asn Glu Ile Met Ala Asn Ser Leu Asp Tyr Asn Glu Arg Leu 
    130                 135                 140                 


Trp Ala Trp Glu Ser Trp Arg Ser Glu Val Gly Lys Gln Leu Arg Pro 
145                 150                 155                 160 


Leu Tyr Glu Glu Tyr Val Val Leu Lys Asn Glu Met Ala Arg Ala Asn 
                165                 170                 175     


His Tyr Glu Asp Tyr Gly Asp Tyr Trp Arg Gly Asp Tyr Glu Val Asn 
            180                 185                 190         


Gly Val Asp Gly Tyr Asp Tyr Ser Arg Gly Gln Leu Ile Glu Asp Val 
        195                 200                 205             


Glu His Thr Phe Glu Glu Ile Lys Pro Leu Tyr Glu His Leu His Ala 
    210                 215                 220                 


Tyr Val Arg Ala Lys Leu Met Asn Ala Tyr Pro Ser Tyr Ile Ser Pro 
225                 230                 235                 240 


Ile Gly Cys Leu Pro Ala His Leu Leu Gly Asp Met Trp Gly Arg Phe 
                245                 250                 255     


Trp Thr Asn Leu Tyr Ser Leu Thr Val Pro Phe Gly Gln Lys Pro Asn 
            260                 265                 270         


Ile Asp Val Thr Asp Ala Met Val Asp Gln Ala Trp Asp Ala Gln Arg 
        275                 280                 285             


Ile Phe Lys Glu Ala Glu Lys Phe Phe Val Ser Val Gly Leu Pro Asn 
    290                 295                 300                 


Met Thr Gln Gly Phe Trp Glu Asn Ser Met Leu Thr Asp Pro Gly Asn 
305                 310                 315                 320 


Val Gln Lys Ala Val Cys His Pro Thr Ala Trp Asp Leu Gly Lys Gly 
                325                 330                 335     


Asp Phe Arg Ile Leu Met Cys Thr Lys Val Thr Met Asp Asp Phe Leu 
            340                 345                 350         


Thr Ala His His Glu Met Gly His Ile Gln Tyr Asp Met Ala Tyr Ala 
        355                 360                 365             


Ala Gln Pro Phe Leu Leu Arg Asn Gly Ala Asn Glu Gly Phe His Glu 
    370                 375                 380                 


Ala Val Gly Glu Ile Met Ser Leu Ser Ala Ala Thr Pro Lys His Leu 
385                 390                 395                 400 


Lys Ser Ile Gly Leu Leu Ser Pro Asp Phe Gln Glu Asp Asn Glu Thr 
                405                 410                 415     


Glu Ile Asn Phe Leu Leu Lys Gln Ala Leu Thr Ile Val Gly Thr Leu 
            420                 425                 430         


Pro Phe Thr Tyr Met Leu Glu Lys Trp Arg Trp Met Val Phe Lys Gly 
        435                 440                 445             


Glu Ile Pro Lys Asp Gln Trp Met Lys Lys Trp Trp Glu Met Lys Arg 
    450                 455                 460                 


Glu Ile Val Gly Val Val Glu Pro Val Pro His Asp Glu Thr Tyr Cys 
465                 470                 475                 480 


Asp Pro Ala Ser Leu Phe His Val Ser Asn Asp Tyr Ser Phe Ile Arg 
                485                 490                 495     


Tyr Tyr Thr Arg Thr Leu Tyr Gln Phe Gln Phe Gln Glu Ala Leu Cys 
            500                 505                 510         


Gln Ala Ala Lys His Glu Gly Pro Leu His Lys Cys Asp Ile Ser Asn 
        515                 520                 525             


Ser Thr Glu Ala Gly Gln Lys Leu Phe Asn Met Leu Arg Leu Gly Lys 
    530                 535                 540                 


Ser Glu Pro Trp Thr Leu Ala Leu Glu Asn Val Val Gly Ala Lys Asn 
545                 550                 555                 560 


Met Asn Val Arg Pro Leu Leu Asn Tyr Phe Glu Pro Leu Phe Thr Trp 
                565                 570                 575     


Leu Lys Asp Gln Asn Lys Asn Ser Phe Val Gly Trp Ser Thr Asp Trp 
            580                 585                 590         


Ser Pro Tyr Ala Asp 
        595         


<210>  5
<211>  597
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Fragment of Peptidase domain (19-615) of human ACE-2 with 
       substitution Glu375Gln

<400>  5

Ser Thr Ile Glu Glu Gln Ala Lys Thr Phe Leu Asp Lys Phe Asn His 
1               5                   10                  15      


Glu Ala Glu Asp Leu Phe Tyr Gln Ser Ser Leu Ala Ser Trp Asn Tyr 
            20                  25                  30          


Asn Thr Asn Ile Thr Glu Glu Asn Val Gln Asn Met Asn Asn Ala Gly 
        35                  40                  45              


Asp Lys Trp Ser Ala Phe Leu Lys Glu Gln Ser Thr Leu Ala Gln Met 
    50                  55                  60                  


Tyr Pro Leu Gln Glu Ile Gln Asn Leu Thr Val Lys Leu Gln Leu Gln 
65                  70                  75                  80  


Ala Leu Gln Gln Asn Gly Ser Ser Val Leu Ser Glu Asp Lys Ser Lys 
                85                  90                  95      


Arg Leu Asn Thr Ile Leu Asn Thr Met Ser Thr Ile Tyr Ser Thr Gly 
            100                 105                 110         


Lys Val Cys Asn Pro Asp Asn Pro Gln Glu Cys Leu Leu Leu Glu Pro 
        115                 120                 125             


Gly Leu Asn Glu Ile Met Ala Asn Ser Leu Asp Tyr Asn Glu Arg Leu 
    130                 135                 140                 


Trp Ala Trp Glu Ser Trp Arg Ser Glu Val Gly Lys Gln Leu Arg Pro 
145                 150                 155                 160 


Leu Tyr Glu Glu Tyr Val Val Leu Lys Asn Glu Met Ala Arg Ala Asn 
                165                 170                 175     


His Tyr Glu Asp Tyr Gly Asp Tyr Trp Arg Gly Asp Tyr Glu Val Asn 
            180                 185                 190         


Gly Val Asp Gly Tyr Asp Tyr Ser Arg Gly Gln Leu Ile Glu Asp Val 
        195                 200                 205             


Glu His Thr Phe Glu Glu Ile Lys Pro Leu Tyr Glu His Leu His Ala 
    210                 215                 220                 


Tyr Val Arg Ala Lys Leu Met Asn Ala Tyr Pro Ser Tyr Ile Ser Pro 
225                 230                 235                 240 


Ile Gly Cys Leu Pro Ala His Leu Leu Gly Asp Met Trp Gly Arg Phe 
                245                 250                 255     


Trp Thr Asn Leu Tyr Ser Leu Thr Val Pro Phe Gly Gln Lys Pro Asn 
            260                 265                 270         


Ile Asp Val Thr Asp Ala Met Val Asp Gln Ala Trp Asp Ala Gln Arg 
        275                 280                 285             


Ile Phe Lys Glu Ala Glu Lys Phe Phe Val Ser Val Gly Leu Pro Asn 
    290                 295                 300                 


Met Thr Gln Gly Phe Trp Glu Asn Ser Met Leu Thr Asp Pro Gly Asn 
305                 310                 315                 320 


Val Gln Lys Ala Val Cys His Pro Thr Ala Trp Asp Leu Gly Lys Gly 
                325                 330                 335     


Asp Phe Arg Ile Leu Met Cys Thr Lys Val Thr Met Asp Asp Phe Leu 
            340                 345                 350         


Thr Ala His His Gln Met Gly His Ile Gln Tyr Asp Met Ala Tyr Ala 
        355                 360                 365             


Ala Gln Pro Phe Leu Leu Arg Asn Gly Ala Asn Glu Gly Phe His Glu 
    370                 375                 380                 


Ala Val Gly Glu Ile Met Ser Leu Ser Ala Ala Thr Pro Lys His Leu 
385                 390                 395                 400 


Lys Ser Ile Gly Leu Leu Ser Pro Asp Phe Gln Glu Asp Asn Glu Thr 
                405                 410                 415     


Glu Ile Asn Phe Leu Leu Lys Gln Ala Leu Thr Ile Val Gly Thr Leu 
            420                 425                 430         


Pro Phe Thr Tyr Met Leu Glu Lys Trp Arg Trp Met Val Phe Lys Gly 
        435                 440                 445             


Glu Ile Pro Lys Asp Gln Trp Met Lys Lys Trp Trp Glu Met Lys Arg 
    450                 455                 460                 


Glu Ile Val Gly Val Val Glu Pro Val Pro His Asp Glu Thr Tyr Cys 
465                 470                 475                 480 


Asp Pro Ala Ser Leu Phe His Val Ser Asn Asp Tyr Ser Phe Ile Arg 
                485                 490                 495     


Tyr Tyr Thr Arg Thr Leu Tyr Gln Phe Gln Phe Gln Glu Ala Leu Cys 
            500                 505                 510         


Gln Ala Ala Lys His Glu Gly Pro Leu His Lys Cys Asp Ile Ser Asn 
        515                 520                 525             


Ser Thr Glu Ala Gly Gln Lys Leu Phe Asn Met Leu Arg Leu Gly Lys 
    530                 535                 540                 


Ser Glu Pro Trp Thr Leu Ala Leu Glu Asn Val Val Gly Ala Lys Asn 
545                 550                 555                 560 


Met Asn Val Arg Pro Leu Leu Asn Tyr Phe Glu Pro Leu Phe Thr Trp 
                565                 570                 575     


Leu Lys Asp Gln Asn Lys Asn Ser Phe Val Gly Trp Ser Thr Asp Trp 
            580                 585                 590         


Ser Pro Tyr Ala Asp 
        595         


<210>  6
<211>  614
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Peptidase domain (2-165) of human ACE-2

<400>  6

Ser Ser Ser Ser Trp Leu Leu Leu Ser Leu Val Ala Val Thr Ala Ala 
1               5                   10                  15      


Gln Ser Thr Ile Glu Glu Gln Ala Lys Thr Phe Leu Asp Lys Phe Asn 
            20                  25                  30          


His Glu Ala Glu Asp Leu Phe Tyr Gln Ser Ser Leu Ala Ser Trp Asn 
        35                  40                  45              


Tyr Asn Thr Asn Ile Thr Glu Glu Asn Val Gln Asn Met Asn Asn Ala 
    50                  55                  60                  


Gly Asp Lys Trp Ser Ala Phe Leu Lys Glu Gln Ser Thr Leu Ala Gln 
65                  70                  75                  80  


Met Tyr Pro Leu Gln Glu Ile Gln Asn Leu Thr Val Lys Leu Gln Leu 
                85                  90                  95      


Gln Ala Leu Gln Gln Asn Gly Ser Ser Val Leu Ser Glu Asp Lys Ser 
            100                 105                 110         


Lys Arg Leu Asn Thr Ile Leu Asn Thr Met Ser Thr Ile Tyr Ser Thr 
        115                 120                 125             


Gly Lys Val Cys Asn Pro Asp Asn Pro Gln Glu Cys Leu Leu Leu Glu 
    130                 135                 140                 


Pro Gly Leu Asn Glu Ile Met Ala Asn Ser Leu Asp Tyr Asn Glu Arg 
145                 150                 155                 160 


Leu Trp Ala Trp Glu Ser Trp Arg Ser Glu Val Gly Lys Gln Leu Arg 
                165                 170                 175     


Pro Leu Tyr Glu Glu Tyr Val Val Leu Lys Asn Glu Met Ala Arg Ala 
            180                 185                 190         


Asn His Tyr Glu Asp Tyr Gly Asp Tyr Trp Arg Gly Asp Tyr Glu Val 
        195                 200                 205             


Asn Gly Val Asp Gly Tyr Asp Tyr Ser Arg Gly Gln Leu Ile Glu Asp 
    210                 215                 220                 


Val Glu His Thr Phe Glu Glu Ile Lys Pro Leu Tyr Glu His Leu His 
225                 230                 235                 240 


Ala Tyr Val Arg Ala Lys Leu Met Asn Ala Tyr Pro Ser Tyr Ile Ser 
                245                 250                 255     


Pro Ile Gly Cys Leu Pro Ala His Leu Leu Gly Asp Met Trp Gly Arg 
            260                 265                 270         


Phe Trp Thr Asn Leu Tyr Ser Leu Thr Val Pro Phe Gly Gln Lys Pro 
        275                 280                 285             


Asn Ile Asp Val Thr Asp Ala Met Val Asp Gln Ala Trp Asp Ala Gln 
    290                 295                 300                 


Arg Ile Phe Lys Glu Ala Glu Lys Phe Phe Val Ser Val Gly Leu Pro 
305                 310                 315                 320 


Asn Met Thr Gln Gly Phe Trp Glu Asn Ser Met Leu Thr Asp Pro Gly 
                325                 330                 335     


Asn Val Gln Lys Ala Val Cys His Pro Thr Ala Trp Asp Leu Gly Lys 
            340                 345                 350         


Gly Asp Phe Arg Ile Leu Met Cys Thr Lys Val Thr Met Asp Asp Phe 
        355                 360                 365             


Leu Thr Ala His His Glu Met Gly His Ile Gln Tyr Asp Met Ala Tyr 
    370                 375                 380                 


Ala Ala Gln Pro Phe Leu Leu Arg Asn Gly Ala Asn Glu Gly Phe His 
385                 390                 395                 400 


Glu Ala Val Gly Glu Ile Met Ser Leu Ser Ala Ala Thr Pro Lys His 
                405                 410                 415     


Leu Lys Ser Ile Gly Leu Leu Ser Pro Asp Phe Gln Glu Asp Asn Glu 
            420                 425                 430         


Thr Glu Ile Asn Phe Leu Leu Lys Gln Ala Leu Thr Ile Val Gly Thr 
        435                 440                 445             


Leu Pro Phe Thr Tyr Met Leu Glu Lys Trp Arg Trp Met Val Phe Lys 
    450                 455                 460                 


Gly Glu Ile Pro Lys Asp Gln Trp Met Lys Lys Trp Trp Glu Met Lys 
465                 470                 475                 480 


Arg Glu Ile Val Gly Val Val Glu Pro Val Pro His Asp Glu Thr Tyr 
                485                 490                 495     


Cys Asp Pro Ala Ser Leu Phe His Val Ser Asn Asp Tyr Ser Phe Ile 
            500                 505                 510         


Arg Tyr Tyr Thr Arg Thr Leu Tyr Gln Phe Gln Phe Gln Glu Ala Leu 
        515                 520                 525             


Cys Gln Ala Ala Lys His Glu Gly Pro Leu His Lys Cys Asp Ile Ser 
    530                 535                 540                 


Asn Ser Thr Glu Ala Gly Gln Lys Leu Phe Asn Met Leu Arg Leu Gly 
545                 550                 555                 560 


Lys Ser Glu Pro Trp Thr Leu Ala Leu Glu Asn Val Val Gly Ala Lys 
                565                 570                 575     


Asn Met Asn Val Arg Pro Leu Leu Asn Tyr Phe Glu Pro Leu Phe Thr 
            580                 585                 590         


Trp Leu Lys Asp Gln Asn Lys Asn Ser Phe Val Gly Trp Ser Thr Asp 
        595                 600                 605             


Trp Ser Pro Tyr Ala Asp 
    610                 


<210>  7
<211>  165
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Fragment combination of Peptidase domain (19-103;301-365) of 
       ACE-2

<400>  7

Ser Thr Ile Glu Glu Gln Ala Lys Thr Phe Leu Asp Lys Phe Asn His 
1               5                   10                  15      


Glu Ala Glu Asp Leu Phe Tyr Gln Ser Ser Leu Ala Ser Trp Asn Tyr 
            20                  25                  30          


Asn Thr Asn Ile Thr Glu Glu Asn Val Gln Asn Met Asn Asn Ala Gly 
        35                  40                  45              


Asp Lys Trp Ser Ala Phe Leu Lys Glu Gln Ser Thr Leu Ala Gln Met 
    50                  55                  60                  


Tyr Pro Leu Gln Glu Ile Gln Asn Leu Thr Val Lys Leu Gln Leu Gln 
65                  70                  75                  80  


Ala Leu Gln Gln Asn Gly Ser Ala Gly Ser Ala Ala Gly Gly Ser Ala 
                85                  90                  95      


Gly Ser Ala Ala Gly Ala Trp Asp Ala Gln Arg Ile Phe Lys Glu Ala 
            100                 105                 110         


Glu Lys Phe Phe Val Ser Val Gly Leu Pro Asn Met Thr Gln Gly Phe 
        115                 120                 125             


Trp Glu Asn Ser Met Leu Thr Asp Pro Gly Asn Val Gln Lys Ala Val 
    130                 135                 140                 


Cys His Pro Thr Ala Trp Asp Leu Gly Lys Gly Asp Phe Arg Ile Leu 
145                 150                 155                 160 


Met Cys Thr Lys Val 
                165 


<210>  8
<211>  85
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Fragment of Peptidase domain (19-103) of ACE-2

<400>  8

Ser Thr Ile Glu Glu Gln Ala Lys Thr Phe Leu Asp Lys Phe Asn His 
1               5                   10                  15      


Glu Ala Glu Asp Leu Phe Tyr Gln Ser Ser Leu Ala Ser Trp Asn Tyr 
            20                  25                  30          


Asn Thr Asn Ile Thr Glu Glu Asn Val Gln Asn Met Asn Asn Ala Gly 
        35                  40                  45              


Asp Lys Trp Ser Ala Phe Leu Lys Glu Gln Ser Thr Leu Ala Gln Met 
    50                  55                  60                  


Tyr Pro Leu Gln Glu Ile Gln Asn Leu Thr Val Lys Leu Gln Leu Gln 
65                  70                  75                  80  


Ala Leu Gln Gln Asn 
                85  


<210>  9
<211>  114
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Fragment of Peptidase domain (19-132) of ACE-2

<400>  9

Ser Thr Ile Glu Glu Gln Ala Lys Thr Phe Leu Asp Lys Phe Asn His 
1               5                   10                  15      


Glu Ala Glu Asp Leu Phe Tyr Gln Ser Ser Leu Ala Ser Trp Asn Tyr 
            20                  25                  30          


Asn Thr Asn Ile Thr Glu Glu Asn Val Gln Asn Met Asn Asn Ala Gly 
        35                  40                  45              


Asp Lys Trp Ser Ala Phe Leu Lys Glu Gln Ser Thr Leu Ala Gln Met 
    50                  55                  60                  


Tyr Pro Leu Gln Glu Ile Gln Asn Leu Thr Val Lys Leu Gln Leu Gln 
65                  70                  75                  80  


Ala Leu Gln Gln Asn Gly Ser Ser Val Leu Ser Glu Asp Lys Ser Lys 
                85                  90                  95      


Arg Leu Asn Thr Ile Leu Asn Thr Met Ser Thr Ile Tyr Ser Thr Gly 
            100                 105                 110         


Lys Val 
        


<210>  10
<211>  610
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  (3)_ACE2_19-615_E375Q

<400>  10

Met Ser Thr Ile Glu Glu Gln Ala Lys Thr Phe Leu Asp Lys Phe Asn 
1               5                   10                  15      


His Glu Ala Glu Asp Leu Phe Tyr Gln Ser Ser Leu Ala Ser Trp Asn 
            20                  25                  30          


Tyr Asn Thr Asn Ile Thr Glu Glu Asn Val Gln Asn Met Asn Asn Ala 
        35                  40                  45              


Gly Asp Lys Trp Ser Ala Phe Leu Lys Glu Gln Ser Thr Leu Ala Gln 
    50                  55                  60                  


Met Tyr Pro Leu Gln Glu Ile Gln Asn Leu Thr Val Lys Leu Gln Leu 
65                  70                  75                  80  


Gln Ala Leu Gln Gln Asn Gly Ser Ser Val Leu Ser Glu Asp Lys Ser 
                85                  90                  95      


Lys Arg Leu Asn Thr Ile Leu Asn Thr Met Ser Thr Ile Tyr Ser Thr 
            100                 105                 110         


Gly Lys Val Cys Asn Pro Asp Asn Pro Gln Glu Cys Leu Leu Leu Glu 
        115                 120                 125             


Pro Gly Leu Asn Glu Ile Met Ala Asn Ser Leu Asp Tyr Asn Glu Arg 
    130                 135                 140                 


Leu Trp Ala Trp Glu Ser Trp Arg Ser Glu Val Gly Lys Gln Leu Arg 
145                 150                 155                 160 


Pro Leu Tyr Glu Glu Tyr Val Val Leu Lys Asn Glu Met Ala Arg Ala 
                165                 170                 175     


Asn His Tyr Glu Asp Tyr Gly Asp Tyr Trp Arg Gly Asp Tyr Glu Val 
            180                 185                 190         


Asn Gly Val Asp Gly Tyr Asp Tyr Ser Arg Gly Gln Leu Ile Glu Asp 
        195                 200                 205             


Val Glu His Thr Phe Glu Glu Ile Lys Pro Leu Tyr Glu His Leu His 
    210                 215                 220                 


Ala Tyr Val Arg Ala Lys Leu Met Asn Ala Tyr Pro Ser Tyr Ile Ser 
225                 230                 235                 240 


Pro Ile Gly Cys Leu Pro Ala His Leu Leu Gly Asp Met Trp Gly Arg 
                245                 250                 255     


Phe Trp Thr Asn Leu Tyr Ser Leu Thr Val Pro Phe Gly Gln Lys Pro 
            260                 265                 270         


Asn Ile Asp Val Thr Asp Ala Met Val Asp Gln Ala Trp Asp Ala Gln 
        275                 280                 285             


Arg Ile Phe Lys Glu Ala Glu Lys Phe Phe Val Ser Val Gly Leu Pro 
    290                 295                 300                 


Asn Met Thr Gln Gly Phe Trp Glu Asn Ser Met Leu Thr Asp Pro Gly 
305                 310                 315                 320 


Asn Val Gln Lys Ala Val Cys His Pro Thr Ala Trp Asp Leu Gly Lys 
                325                 330                 335     


Gly Asp Phe Arg Ile Leu Met Cys Thr Lys Val Thr Met Asp Asp Phe 
            340                 345                 350         


Leu Thr Ala His His Gln Met Gly His Ile Gln Tyr Asp Met Ala Tyr 
        355                 360                 365             


Ala Ala Gln Pro Phe Leu Leu Arg Asn Gly Ala Asn Glu Gly Phe His 
    370                 375                 380                 


Glu Ala Val Gly Glu Ile Met Ser Leu Ser Ala Ala Thr Pro Lys His 
385                 390                 395                 400 


Leu Lys Ser Ile Gly Leu Leu Ser Pro Asp Phe Gln Glu Asp Asn Glu 
                405                 410                 415     


Thr Glu Ile Asn Phe Leu Leu Lys Gln Ala Leu Thr Ile Val Gly Thr 
            420                 425                 430         


Leu Pro Phe Thr Tyr Met Leu Glu Lys Trp Arg Trp Met Val Phe Lys 
        435                 440                 445             


Gly Glu Ile Pro Lys Asp Gln Trp Met Lys Lys Trp Trp Glu Met Lys 
    450                 455                 460                 


Arg Glu Ile Val Gly Val Val Glu Pro Val Pro His Asp Glu Thr Tyr 
465                 470                 475                 480 


Cys Asp Pro Ala Ser Leu Phe His Val Ser Asn Asp Tyr Ser Phe Ile 
                485                 490                 495     


Arg Tyr Tyr Thr Arg Thr Leu Tyr Gln Phe Gln Phe Gln Glu Ala Leu 
            500                 505                 510         


Cys Gln Ala Ala Lys His Glu Gly Pro Leu His Lys Cys Asp Ile Ser 
        515                 520                 525             


Asn Ser Thr Glu Ala Gly Gln Lys Leu Phe Asn Met Leu Arg Leu Gly 
    530                 535                 540                 


Lys Ser Glu Pro Trp Thr Leu Ala Leu Glu Asn Val Val Gly Ala Lys 
545                 550                 555                 560 


Asn Met Asn Val Arg Pro Leu Leu Asn Tyr Phe Glu Pro Leu Phe Thr 
                565                 570                 575     


Trp Leu Lys Asp Gln Asn Lys Asn Ser Phe Val Gly Trp Ser Thr Asp 
            580                 585                 590         


Trp Ser Pro Tyr Ala Asp Gly Gly Gly Ser His His His His His His 
        595                 600                 605             


His His 
    610 


<210>  11
<211>  178
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  (4)_ACE_2_19-103;301-365

<400>  11

Met Ser Thr Ile Glu Glu Gln Ala Lys Thr Phe Leu Asp Lys Phe Asn 
1               5                   10                  15      


His Glu Ala Glu Asp Leu Phe Tyr Gln Ser Ser Leu Ala Ser Trp Asn 
            20                  25                  30          


Tyr Asn Thr Asn Ile Thr Glu Glu Asn Val Gln Asn Met Asn Asn Ala 
        35                  40                  45              


Gly Asp Lys Trp Ser Ala Phe Leu Lys Glu Gln Ser Thr Leu Ala Gln 
    50                  55                  60                  


Met Tyr Pro Leu Gln Glu Ile Gln Asn Leu Thr Val Lys Leu Gln Leu 
65                  70                  75                  80  


Gln Ala Leu Gln Gln Asn Gly Ser Ala Gly Ser Ala Ala Gly Gly Ser 
                85                  90                  95      


Ala Gly Ser Ala Ala Gly Ala Trp Asp Ala Gln Arg Ile Phe Lys Glu 
            100                 105                 110         


Ala Glu Lys Phe Phe Val Ser Val Gly Leu Pro Asn Met Thr Gln Gly 
        115                 120                 125             


Phe Trp Glu Asn Ser Met Leu Thr Asp Pro Gly Asn Val Gln Lys Ala 
    130                 135                 140                 


Val Cys His Pro Thr Ala Trp Asp Leu Gly Lys Gly Asp Phe Arg Ile 
145                 150                 155                 160 


Leu Met Cys Thr Lys Val Gly Gly Gly Ser His His His His His His 
                165                 170                 175     


His His 
        


<210>  12
<211>  610
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  (9)_ACE2_19-615

<400>  12

Met Ser Thr Ile Glu Glu Gln Ala Lys Thr Phe Leu Asp Lys Phe Asn 
1               5                   10                  15      


His Glu Ala Glu Asp Leu Phe Tyr Gln Ser Ser Leu Ala Ser Trp Asn 
            20                  25                  30          


Tyr Asn Thr Asn Ile Thr Glu Glu Asn Val Gln Asn Met Asn Asn Ala 
        35                  40                  45              


Gly Asp Lys Trp Ser Ala Phe Leu Lys Glu Gln Ser Thr Leu Ala Gln 
    50                  55                  60                  


Met Tyr Pro Leu Gln Glu Ile Gln Asn Leu Thr Val Lys Leu Gln Leu 
65                  70                  75                  80  


Gln Ala Leu Gln Gln Asn Gly Ser Ser Val Leu Ser Glu Asp Lys Ser 
                85                  90                  95      


Lys Arg Leu Asn Thr Ile Leu Asn Thr Met Ser Thr Ile Tyr Ser Thr 
            100                 105                 110         


Gly Lys Val Cys Asn Pro Asp Asn Pro Gln Glu Cys Leu Leu Leu Glu 
        115                 120                 125             


Pro Gly Leu Asn Glu Ile Met Ala Asn Ser Leu Asp Tyr Asn Glu Arg 
    130                 135                 140                 


Leu Trp Ala Trp Glu Ser Trp Arg Ser Glu Val Gly Lys Gln Leu Arg 
145                 150                 155                 160 


Pro Leu Tyr Glu Glu Tyr Val Val Leu Lys Asn Glu Met Ala Arg Ala 
                165                 170                 175     


Asn His Tyr Glu Asp Tyr Gly Asp Tyr Trp Arg Gly Asp Tyr Glu Val 
            180                 185                 190         


Asn Gly Val Asp Gly Tyr Asp Tyr Ser Arg Gly Gln Leu Ile Glu Asp 
        195                 200                 205             


Val Glu His Thr Phe Glu Glu Ile Lys Pro Leu Tyr Glu His Leu His 
    210                 215                 220                 


Ala Tyr Val Arg Ala Lys Leu Met Asn Ala Tyr Pro Ser Tyr Ile Ser 
225                 230                 235                 240 


Pro Ile Gly Cys Leu Pro Ala His Leu Leu Gly Asp Met Trp Gly Arg 
                245                 250                 255     


Phe Trp Thr Asn Leu Tyr Ser Leu Thr Val Pro Phe Gly Gln Lys Pro 
            260                 265                 270         


Asn Ile Asp Val Thr Asp Ala Met Val Asp Gln Ala Trp Asp Ala Gln 
        275                 280                 285             


Arg Ile Phe Lys Glu Ala Glu Lys Phe Phe Val Ser Val Gly Leu Pro 
    290                 295                 300                 


Asn Met Thr Gln Gly Phe Trp Glu Asn Ser Met Leu Thr Asp Pro Gly 
305                 310                 315                 320 


Asn Val Gln Lys Ala Val Cys His Pro Thr Ala Trp Asp Leu Gly Lys 
                325                 330                 335     


Gly Asp Phe Arg Ile Leu Met Cys Thr Lys Val Thr Met Asp Asp Phe 
            340                 345                 350         


Leu Thr Ala His His Glu Met Gly His Ile Gln Tyr Asp Met Ala Tyr 
        355                 360                 365             


Ala Ala Gln Pro Phe Leu Leu Arg Asn Gly Ala Asn Glu Gly Phe His 
    370                 375                 380                 


Glu Ala Val Gly Glu Ile Met Ser Leu Ser Ala Ala Thr Pro Lys His 
385                 390                 395                 400 


Leu Lys Ser Ile Gly Leu Leu Ser Pro Asp Phe Gln Glu Asp Asn Glu 
                405                 410                 415     


Thr Glu Ile Asn Phe Leu Leu Lys Gln Ala Leu Thr Ile Val Gly Thr 
            420                 425                 430         


Leu Pro Phe Thr Tyr Met Leu Glu Lys Trp Arg Trp Met Val Phe Lys 
        435                 440                 445             


Gly Glu Ile Pro Lys Asp Gln Trp Met Lys Lys Trp Trp Glu Met Lys 
    450                 455                 460                 


Arg Glu Ile Val Gly Val Val Glu Pro Val Pro His Asp Glu Thr Tyr 
465                 470                 475                 480 


Cys Asp Pro Ala Ser Leu Phe His Val Ser Asn Asp Tyr Ser Phe Ile 
                485                 490                 495     


Arg Tyr Tyr Thr Arg Thr Leu Tyr Gln Phe Gln Phe Gln Glu Ala Leu 
            500                 505                 510         


Cys Gln Ala Ala Lys His Glu Gly Pro Leu His Lys Cys Asp Ile Ser 
        515                 520                 525             


Asn Ser Thr Glu Ala Gly Gln Lys Leu Phe Asn Met Leu Arg Leu Gly 
    530                 535                 540                 


Lys Ser Glu Pro Trp Thr Leu Ala Leu Glu Asn Val Val Gly Ala Lys 
545                 550                 555                 560 


Asn Met Asn Val Arg Pro Leu Leu Asn Tyr Phe Glu Pro Leu Phe Thr 
                565                 570                 575     


Trp Leu Lys Asp Gln Asn Lys Asn Ser Phe Val Gly Trp Ser Thr Asp 
            580                 585                 590         


Trp Ser Pro Tyr Ala Asp Gly Gly Gly Ser His His His His His His 
        595                 600                 605             


His His 
    610 


<210>  13
<211>  98
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  (1)_ACE2_19-103

<400>  13

Met Ser Thr Ile Glu Glu Gln Ala Lys Thr Phe Leu Asp Lys Phe Asn 
1               5                   10                  15      


His Glu Ala Glu Asp Leu Phe Tyr Gln Ser Ser Leu Ala Ser Trp Asn 
            20                  25                  30          


Tyr Asn Thr Asn Ile Thr Glu Glu Asn Val Gln Asn Met Asn Asn Ala 
        35                  40                  45              


Gly Asp Lys Trp Ser Ala Phe Leu Lys Glu Gln Ser Thr Leu Ala Gln 
    50                  55                  60                  


Met Tyr Pro Leu Gln Glu Ile Gln Asn Leu Thr Val Lys Leu Gln Leu 
65                  70                  75                  80  


Gln Ala Leu Gln Gln Asn Gly Gly Gly Ser His His His His His His 
                85                  90                  95      


His His 
        


<210>  14
<211>  127
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  (8)_ACE2_19-132

<400>  14

Met Ser Thr Ile Glu Glu Gln Ala Lys Thr Phe Leu Asp Lys Phe Asn 
1               5                   10                  15      


His Glu Ala Glu Asp Leu Phe Tyr Gln Ser Ser Leu Ala Ser Trp Asn 
            20                  25                  30          


Tyr Asn Thr Asn Ile Thr Glu Glu Asn Val Gln Asn Met Asn Asn Ala 
        35                  40                  45              


Gly Asp Lys Trp Ser Ala Phe Leu Lys Glu Gln Ser Thr Leu Ala Gln 
    50                  55                  60                  


Met Tyr Pro Leu Gln Glu Ile Gln Asn Leu Thr Val Lys Leu Gln Leu 
65                  70                  75                  80  


Gln Ala Leu Gln Gln Asn Gly Ser Ser Val Leu Ser Glu Asp Lys Ser 
                85                  90                  95      


Lys Arg Leu Asn Thr Ile Leu Asn Thr Met Ser Thr Ile Tyr Ser Thr 
            100                 105                 110         


Gly Lys Val Gly Gly Gly Ser His His His His His His His His 
        115                 120                 125         


<210>  15
<211>  65
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Fragment 301-365 of Peptidase domain (1-615) of human ACE-2

<400>  15

Ala Trp Asp Ala Gln Arg Ile Phe Lys Glu Ala Glu Lys Phe Phe Val 
1               5                   10                  15      


Ser Val Gly Leu Pro Asn Met Thr Gln Gly Phe Trp Glu Asn Ser Met 
            20                  25                  30          


Leu Thr Asp Pro Gly Asn Val Gln Lys Ala Val Cys His Pro Thr Ala 
        35                  40                  45              


Trp Asp Leu Gly Lys Gly Asp Phe Arg Ile Leu Met Cys Thr Lys Val 
    50                  55                  60                  


Thr 
65  


<210>  16
<211>  17
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Linker 1

<400>  16

Asn Gly Ser Ala Gly Ser Ala Ala Gly Gly Ser Ala Gly Ser Ala Ala 
1               5                   10                  15      


Gly 
    


<210>  17
<211>  584
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Fragment of Peptidase domain (19-602) of human ACE-2

<400>  17

Ser Thr Ile Glu Glu Gln Ala Lys Thr Phe Leu Asp Lys Phe Asn His 
1               5                   10                  15      


Glu Ala Glu Asp Leu Phe Tyr Gln Ser Ser Leu Ala Ser Trp Asn Tyr 
            20                  25                  30          


Asn Thr Asn Ile Thr Glu Glu Asn Val Gln Asn Met Asn Asn Ala Gly 
        35                  40                  45              


Asp Lys Trp Ser Ala Phe Leu Lys Glu Gln Ser Thr Leu Ala Gln Met 
    50                  55                  60                  


Tyr Pro Leu Gln Glu Ile Gln Asn Leu Thr Val Lys Leu Gln Leu Gln 
65                  70                  75                  80  


Ala Leu Gln Gln Asn Gly Ser Ser Val Leu Ser Glu Asp Lys Ser Lys 
                85                  90                  95      


Arg Leu Asn Thr Ile Leu Asn Thr Met Ser Thr Ile Tyr Ser Thr Gly 
            100                 105                 110         


Lys Val Cys Asn Pro Asp Asn Pro Gln Glu Cys Leu Leu Leu Glu Pro 
        115                 120                 125             


Gly Leu Asn Glu Ile Met Ala Asn Ser Leu Asp Tyr Asn Glu Arg Leu 
    130                 135                 140                 


Trp Ala Trp Glu Ser Trp Arg Ser Glu Val Gly Lys Gln Leu Arg Pro 
145                 150                 155                 160 


Leu Tyr Glu Glu Tyr Val Val Leu Lys Asn Glu Met Ala Arg Ala Asn 
                165                 170                 175     


His Tyr Glu Asp Tyr Gly Asp Tyr Trp Arg Gly Asp Tyr Glu Val Asn 
            180                 185                 190         


Gly Val Asp Gly Tyr Asp Tyr Ser Arg Gly Gln Leu Ile Glu Asp Val 
        195                 200                 205             


Glu His Thr Phe Glu Glu Ile Lys Pro Leu Tyr Glu His Leu His Ala 
    210                 215                 220                 


Tyr Val Arg Ala Lys Leu Met Asn Ala Tyr Pro Ser Tyr Ile Ser Pro 
225                 230                 235                 240 


Ile Gly Cys Leu Pro Ala His Leu Leu Gly Asp Met Trp Gly Arg Phe 
                245                 250                 255     


Trp Thr Asn Leu Tyr Ser Leu Thr Val Pro Phe Gly Gln Lys Pro Asn 
            260                 265                 270         


Ile Asp Val Thr Asp Ala Met Val Asp Gln Ala Trp Asp Ala Gln Arg 
        275                 280                 285             


Ile Phe Lys Glu Ala Glu Lys Phe Phe Val Ser Val Gly Leu Pro Asn 
    290                 295                 300                 


Met Thr Gln Gly Phe Trp Glu Asn Ser Met Leu Thr Asp Pro Gly Asn 
305                 310                 315                 320 


Val Gln Lys Ala Val Cys His Pro Thr Ala Trp Asp Leu Gly Lys Gly 
                325                 330                 335     


Asp Phe Arg Ile Leu Met Cys Thr Lys Val Thr Met Asp Asp Phe Leu 
            340                 345                 350         


Thr Ala His His Glu Met Gly His Ile Gln Tyr Asp Met Ala Tyr Ala 
        355                 360                 365             


Ala Gln Pro Phe Leu Leu Arg Asn Gly Ala Asn Glu Gly Phe His Glu 
    370                 375                 380                 


Ala Val Gly Glu Ile Met Ser Leu Ser Ala Ala Thr Pro Lys His Leu 
385                 390                 395                 400 


Lys Ser Ile Gly Leu Leu Ser Pro Asp Phe Gln Glu Asp Asn Glu Thr 
                405                 410                 415     


Glu Ile Asn Phe Leu Leu Lys Gln Ala Leu Thr Ile Val Gly Thr Leu 
            420                 425                 430         


Pro Phe Thr Tyr Met Leu Glu Lys Trp Arg Trp Met Val Phe Lys Gly 
        435                 440                 445             


Glu Ile Pro Lys Asp Gln Trp Met Lys Lys Trp Trp Glu Met Lys Arg 
    450                 455                 460                 


Glu Ile Val Gly Val Val Glu Pro Val Pro His Asp Glu Thr Tyr Cys 
465                 470                 475                 480 


Asp Pro Ala Ser Leu Phe His Val Ser Asn Asp Tyr Ser Phe Ile Arg 
                485                 490                 495     


Tyr Tyr Thr Arg Thr Leu Tyr Gln Phe Gln Phe Gln Glu Ala Leu Cys 
            500                 505                 510         


Gln Ala Ala Lys His Glu Gly Pro Leu His Lys Cys Asp Ile Ser Asn 
        515                 520                 525             


Ser Thr Glu Ala Gly Gln Lys Leu Phe Asn Met Leu Arg Leu Gly Lys 
    530                 535                 540                 


Ser Glu Pro Trp Thr Leu Ala Leu Glu Asn Val Val Gly Ala Lys Asn 
545                 550                 555                 560 


Met Asn Val Arg Pro Leu Leu Asn Tyr Phe Glu Pro Leu Phe Thr Trp 
                565                 570                 575     


Leu Lys Asp Gln Asn Lys Asn Ser 
            580                 


<210>  18
<211>  584
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Fragment of Peptidase domain (19-602) of human ACE-2 wiht 
       substitution Glu375Gln

<400>  18

Ser Thr Ile Glu Glu Gln Ala Lys Thr Phe Leu Asp Lys Phe Asn His 
1               5                   10                  15      


Glu Ala Glu Asp Leu Phe Tyr Gln Ser Ser Leu Ala Ser Trp Asn Tyr 
            20                  25                  30          


Asn Thr Asn Ile Thr Glu Glu Asn Val Gln Asn Met Asn Asn Ala Gly 
        35                  40                  45              


Asp Lys Trp Ser Ala Phe Leu Lys Glu Gln Ser Thr Leu Ala Gln Met 
    50                  55                  60                  


Tyr Pro Leu Gln Glu Ile Gln Asn Leu Thr Val Lys Leu Gln Leu Gln 
65                  70                  75                  80  


Ala Leu Gln Gln Asn Gly Ser Ser Val Leu Ser Glu Asp Lys Ser Lys 
                85                  90                  95      


Arg Leu Asn Thr Ile Leu Asn Thr Met Ser Thr Ile Tyr Ser Thr Gly 
            100                 105                 110         


Lys Val Cys Asn Pro Asp Asn Pro Gln Glu Cys Leu Leu Leu Glu Pro 
        115                 120                 125             


Gly Leu Asn Glu Ile Met Ala Asn Ser Leu Asp Tyr Asn Glu Arg Leu 
    130                 135                 140                 


Trp Ala Trp Glu Ser Trp Arg Ser Glu Val Gly Lys Gln Leu Arg Pro 
145                 150                 155                 160 


Leu Tyr Glu Glu Tyr Val Val Leu Lys Asn Glu Met Ala Arg Ala Asn 
                165                 170                 175     


His Tyr Glu Asp Tyr Gly Asp Tyr Trp Arg Gly Asp Tyr Glu Val Asn 
            180                 185                 190         


Gly Val Asp Gly Tyr Asp Tyr Ser Arg Gly Gln Leu Ile Glu Asp Val 
        195                 200                 205             


Glu His Thr Phe Glu Glu Ile Lys Pro Leu Tyr Glu His Leu His Ala 
    210                 215                 220                 


Tyr Val Arg Ala Lys Leu Met Asn Ala Tyr Pro Ser Tyr Ile Ser Pro 
225                 230                 235                 240 


Ile Gly Cys Leu Pro Ala His Leu Leu Gly Asp Met Trp Gly Arg Phe 
                245                 250                 255     


Trp Thr Asn Leu Tyr Ser Leu Thr Val Pro Phe Gly Gln Lys Pro Asn 
            260                 265                 270         


Ile Asp Val Thr Asp Ala Met Val Asp Gln Ala Trp Asp Ala Gln Arg 
        275                 280                 285             


Ile Phe Lys Glu Ala Glu Lys Phe Phe Val Ser Val Gly Leu Pro Asn 
    290                 295                 300                 


Met Thr Gln Gly Phe Trp Glu Asn Ser Met Leu Thr Asp Pro Gly Asn 
305                 310                 315                 320 


Val Gln Lys Ala Val Cys His Pro Thr Ala Trp Asp Leu Gly Lys Gly 
                325                 330                 335     


Asp Phe Arg Ile Leu Met Cys Thr Lys Val Thr Met Asp Asp Phe Leu 
            340                 345                 350         


Thr Ala His His Gln Met Gly His Ile Gln Tyr Asp Met Ala Tyr Ala 
        355                 360                 365             


Ala Gln Pro Phe Leu Leu Arg Asn Gly Ala Asn Glu Gly Phe His Glu 
    370                 375                 380                 


Ala Val Gly Glu Ile Met Ser Leu Ser Ala Ala Thr Pro Lys His Leu 
385                 390                 395                 400 


Lys Ser Ile Gly Leu Leu Ser Pro Asp Phe Gln Glu Asp Asn Glu Thr 
                405                 410                 415     


Glu Ile Asn Phe Leu Leu Lys Gln Ala Leu Thr Ile Val Gly Thr Leu 
            420                 425                 430         


Pro Phe Thr Tyr Met Leu Glu Lys Trp Arg Trp Met Val Phe Lys Gly 
        435                 440                 445             


Glu Ile Pro Lys Asp Gln Trp Met Lys Lys Trp Trp Glu Met Lys Arg 
    450                 455                 460                 


Glu Ile Val Gly Val Val Glu Pro Val Pro His Asp Glu Thr Tyr Cys 
465                 470                 475                 480 


Asp Pro Ala Ser Leu Phe His Val Ser Asn Asp Tyr Ser Phe Ile Arg 
                485                 490                 495     


Tyr Tyr Thr Arg Thr Leu Tyr Gln Phe Gln Phe Gln Glu Ala Leu Cys 
            500                 505                 510         


Gln Ala Ala Lys His Glu Gly Pro Leu His Lys Cys Asp Ile Ser Asn 
        515                 520                 525             


Ser Thr Glu Ala Gly Gln Lys Leu Phe Asn Met Leu Arg Leu Gly Lys 
    530                 535                 540                 


Ser Glu Pro Trp Thr Leu Ala Leu Glu Asn Val Val Gly Ala Lys Asn 
545                 550                 555                 560 


Met Asn Val Arg Pro Leu Leu Asn Tyr Phe Glu Pro Leu Phe Thr Trp 
                565                 570                 575     


Leu Lys Asp Gln Asn Lys Asn Ser 
            580                 


<210>  19
<211>  597
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  (2)_ACE_19 - 602_E375Q

<400>  19

Met Ser Thr Ile Glu Glu Gln Ala Lys Thr Phe Leu Asp Lys Phe Asn 
1               5                   10                  15      


His Glu Ala Glu Asp Leu Phe Tyr Gln Ser Ser Leu Ala Ser Trp Asn 
            20                  25                  30          


Tyr Asn Thr Asn Ile Thr Glu Glu Asn Val Gln Asn Met Asn Asn Ala 
        35                  40                  45              


Gly Asp Lys Trp Ser Ala Phe Leu Lys Glu Gln Ser Thr Leu Ala Gln 
    50                  55                  60                  


Met Tyr Pro Leu Gln Glu Ile Gln Asn Leu Thr Val Lys Leu Gln Leu 
65                  70                  75                  80  


Gln Ala Leu Gln Gln Asn Gly Ser Ser Val Leu Ser Glu Asp Lys Ser 
                85                  90                  95      


Lys Arg Leu Asn Thr Ile Leu Asn Thr Met Ser Thr Ile Tyr Ser Thr 
            100                 105                 110         


Gly Lys Val Cys Asn Pro Asp Asn Pro Gln Glu Cys Leu Leu Leu Glu 
        115                 120                 125             


Pro Gly Leu Asn Glu Ile Met Ala Asn Ser Leu Asp Tyr Asn Glu Arg 
    130                 135                 140                 


Leu Trp Ala Trp Glu Ser Trp Arg Ser Glu Val Gly Lys Gln Leu Arg 
145                 150                 155                 160 


Pro Leu Tyr Glu Glu Tyr Val Val Leu Lys Asn Glu Met Ala Arg Ala 
                165                 170                 175     


Asn His Tyr Glu Asp Tyr Gly Asp Tyr Trp Arg Gly Asp Tyr Glu Val 
            180                 185                 190         


Asn Gly Val Asp Gly Tyr Asp Tyr Ser Arg Gly Gln Leu Ile Glu Asp 
        195                 200                 205             


Val Glu His Thr Phe Glu Glu Ile Lys Pro Leu Tyr Glu His Leu His 
    210                 215                 220                 


Ala Tyr Val Arg Ala Lys Leu Met Asn Ala Tyr Pro Ser Tyr Ile Ser 
225                 230                 235                 240 


Pro Ile Gly Cys Leu Pro Ala His Leu Leu Gly Asp Met Trp Gly Arg 
                245                 250                 255     


Phe Trp Thr Asn Leu Tyr Ser Leu Thr Val Pro Phe Gly Gln Lys Pro 
            260                 265                 270         


Asn Ile Asp Val Thr Asp Ala Met Val Asp Gln Ala Trp Asp Ala Gln 
        275                 280                 285             


Arg Ile Phe Lys Glu Ala Glu Lys Phe Phe Val Ser Val Gly Leu Pro 
    290                 295                 300                 


Asn Met Thr Gln Gly Phe Trp Glu Asn Ser Met Leu Thr Asp Pro Gly 
305                 310                 315                 320 


Asn Val Gln Lys Ala Val Cys His Pro Thr Ala Trp Asp Leu Gly Lys 
                325                 330                 335     


Gly Asp Phe Arg Ile Leu Met Cys Thr Lys Val Thr Met Asp Asp Phe 
            340                 345                 350         


Leu Thr Ala His His Gln Met Gly His Ile Gln Tyr Asp Met Ala Tyr 
        355                 360                 365             


Ala Ala Gln Pro Phe Leu Leu Arg Asn Gly Ala Asn Glu Gly Phe His 
    370                 375                 380                 


Glu Ala Val Gly Glu Ile Met Ser Leu Ser Ala Ala Thr Pro Lys His 
385                 390                 395                 400 


Leu Lys Ser Ile Gly Leu Leu Ser Pro Asp Phe Gln Glu Asp Asn Glu 
                405                 410                 415     


Thr Glu Ile Asn Phe Leu Leu Lys Gln Ala Leu Thr Ile Val Gly Thr 
            420                 425                 430         


Leu Pro Phe Thr Tyr Met Leu Glu Lys Trp Arg Trp Met Val Phe Lys 
        435                 440                 445             


Gly Glu Ile Pro Lys Asp Gln Trp Met Lys Lys Trp Trp Glu Met Lys 
    450                 455                 460                 


Arg Glu Ile Val Gly Val Val Glu Pro Val Pro His Asp Glu Thr Tyr 
465                 470                 475                 480 


Cys Asp Pro Ala Ser Leu Phe His Val Ser Asn Asp Tyr Ser Phe Ile 
                485                 490                 495     


Arg Tyr Tyr Thr Arg Thr Leu Tyr Gln Phe Gln Phe Gln Glu Ala Leu 
            500                 505                 510         


Cys Gln Ala Ala Lys His Glu Gly Pro Leu His Lys Cys Asp Ile Ser 
        515                 520                 525             


Asn Ser Thr Glu Ala Gly Gln Lys Leu Phe Asn Met Leu Arg Leu Gly 
    530                 535                 540                 


Lys Ser Glu Pro Trp Thr Leu Ala Leu Glu Asn Val Val Gly Ala Lys 
545                 550                 555                 560 


Asn Met Asn Val Arg Pro Leu Leu Asn Tyr Phe Glu Pro Leu Phe Thr 
                565                 570                 575     


Trp Leu Lys Asp Gln Asn Lys Asn Ser Gly Gly Gly Ser His His His 
            580                 585                 590         


His His His His His 
        595         


<210>  20
<211>  187
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Fragment combination of Peptidase domain (19-103; 301-387)

<400>  20

Ser Thr Ile Glu Glu Gln Ala Lys Thr Phe Leu Asp Lys Phe Asn His 
1               5                   10                  15      


Glu Ala Glu Asp Leu Phe Tyr Gln Ser Ser Leu Ala Ser Trp Asn Tyr 
            20                  25                  30          


Asn Thr Asn Ile Thr Glu Glu Asn Val Gln Asn Met Asn Asn Ala Gly 
        35                  40                  45              


Asp Lys Trp Ser Ala Phe Leu Lys Glu Gln Ser Thr Leu Ala Gln Met 
    50                  55                  60                  


Tyr Pro Leu Gln Glu Ile Gln Asn Leu Thr Val Lys Leu Gln Leu Gln 
65                  70                  75                  80  


Ala Leu Gln Gln Asn Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly 
                85                  90                  95      


Gly Gly Gly Ser Ala Trp Asp Ala Gln Arg Ile Phe Lys Glu Ala Glu 
            100                 105                 110         


Lys Phe Phe Val Ser Val Gly Leu Pro Asn Met Thr Gln Gly Phe Trp 
        115                 120                 125             


Glu Asn Ser Met Leu Thr Asp Pro Gly Asn Val Gln Lys Ala Val Cys 
    130                 135                 140                 


His Pro Thr Ala Trp Asp Leu Gly Lys Gly Asp Phe Arg Ile Leu Met 
145                 150                 155                 160 


Cys Thr Lys Val Thr Met Asp Asp Phe Leu Thr Ala His His Glu Met 
                165                 170                 175     


Gly His Ile Gln Tyr Asp Met Ala Tyr Ala Ala 
            180                 185         


<210>  21
<211>  187
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Fragment combination of Peptidase domain (19-103; 301-387) with 
       substitution Glu375Gln

<400>  21

Ser Thr Ile Glu Glu Gln Ala Lys Thr Phe Leu Asp Lys Phe Asn His 
1               5                   10                  15      


Glu Ala Glu Asp Leu Phe Tyr Gln Ser Ser Leu Ala Ser Trp Asn Tyr 
            20                  25                  30          


Asn Thr Asn Ile Thr Glu Glu Asn Val Gln Asn Met Asn Asn Ala Gly 
        35                  40                  45              


Asp Lys Trp Ser Ala Phe Leu Lys Glu Gln Ser Thr Leu Ala Gln Met 
    50                  55                  60                  


Tyr Pro Leu Gln Glu Ile Gln Asn Leu Thr Val Lys Leu Gln Leu Gln 
65                  70                  75                  80  


Ala Leu Gln Gln Asn Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly 
                85                  90                  95      


Gly Gly Gly Ser Ala Trp Asp Ala Gln Arg Ile Phe Lys Glu Ala Glu 
            100                 105                 110         


Lys Phe Phe Val Ser Val Gly Leu Pro Asn Met Thr Gln Gly Phe Trp 
        115                 120                 125             


Glu Asn Ser Met Leu Thr Asp Pro Gly Asn Val Gln Lys Ala Val Cys 
    130                 135                 140                 


His Pro Thr Ala Trp Asp Leu Gly Lys Gly Asp Phe Arg Ile Leu Met 
145                 150                 155                 160 


Cys Thr Lys Val Thr Met Asp Asp Phe Leu Thr Ala His His Gln Met 
                165                 170                 175     


Gly His Ile Gln Tyr Asp Met Ala Tyr Ala Ala 
            180                 185         


<210>  22
<211>  200
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  (5)_ACE2_19-103;301-387_E375Q

<400>  22

Met Ser Thr Ile Glu Glu Gln Ala Lys Thr Phe Leu Asp Lys Phe Asn 
1               5                   10                  15      


His Glu Ala Glu Asp Leu Phe Tyr Gln Ser Ser Leu Ala Ser Trp Asn 
            20                  25                  30          


Tyr Asn Thr Asn Ile Thr Glu Glu Asn Val Gln Asn Met Asn Asn Ala 
        35                  40                  45              


Gly Asp Lys Trp Ser Ala Phe Leu Lys Glu Gln Ser Thr Leu Ala Gln 
    50                  55                  60                  


Met Tyr Pro Leu Gln Glu Ile Gln Asn Leu Thr Val Lys Leu Gln Leu 
65                  70                  75                  80  


Gln Ala Leu Gln Gln Asn Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
                85                  90                  95      


Gly Gly Gly Gly Ser Ala Trp Asp Ala Gln Arg Ile Phe Lys Glu Ala 
            100                 105                 110         


Glu Lys Phe Phe Val Ser Val Gly Leu Pro Asn Met Thr Gln Gly Phe 
        115                 120                 125             


Trp Glu Asn Ser Met Leu Thr Asp Pro Gly Asn Val Gln Lys Ala Val 
    130                 135                 140                 


Cys His Pro Thr Ala Trp Asp Leu Gly Lys Gly Asp Phe Arg Ile Leu 
145                 150                 155                 160 


Met Cys Thr Lys Val Thr Met Asp Asp Phe Leu Thr Ala His His Gln 
                165                 170                 175     


Met Gly His Ile Gln Tyr Asp Met Ala Tyr Ala Ala Gly Gly Gly Ser 
            180                 185                 190         


His His His His His His His His 
        195                 200 


<210>  23
<211>  87
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Fragment 301-387 of Peptidase domain (1-615) of human ACE-2

<400>  23

Ala Trp Asp Ala Gln Arg Ile Phe Lys Glu Ala Glu Lys Phe Phe Val 
1               5                   10                  15      


Ser Val Gly Leu Pro Asn Met Thr Gln Gly Phe Trp Glu Asn Ser Met 
            20                  25                  30          


Leu Thr Asp Pro Gly Asn Val Gln Lys Ala Val Cys His Pro Thr Ala 
        35                  40                  45              


Trp Asp Leu Gly Lys Gly Asp Phe Arg Ile Leu Met Cys Thr Lys Val 
    50                  55                  60                  


Thr Met Asp Asp Phe Leu Thr Ala His His Glu Met Gly His Ile Gln 
65                  70                  75                  80  


Tyr Asp Met Ala Tyr Ala Ala 
                85          


<210>  24
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Linker 2

<400>  24

Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
1               5                   10                  15  


<210>  25
<211>  225
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Fragment combination of Peptidase domain (19-103; 301-425) of 
       ACE-2

<400>  25

Ser Thr Ile Glu Glu Gln Ala Lys Thr Phe Leu Asp Lys Phe Asn His 
1               5                   10                  15      


Glu Ala Glu Asp Leu Phe Tyr Gln Ser Ser Leu Ala Ser Trp Asn Tyr 
            20                  25                  30          


Asn Thr Asn Ile Thr Glu Glu Asn Val Gln Asn Met Asn Asn Ala Gly 
        35                  40                  45              


Asp Lys Trp Ser Ala Phe Leu Lys Glu Gln Ser Thr Leu Ala Gln Met 
    50                  55                  60                  


Tyr Pro Leu Gln Glu Ile Gln Asn Leu Thr Val Lys Leu Gln Leu Gln 
65                  70                  75                  80  


Ala Leu Gln Gln Asn Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly 
                85                  90                  95      


Gly Gly Gly Ser Ala Trp Asp Ala Gln Arg Ile Phe Lys Glu Ala Glu 
            100                 105                 110         


Lys Phe Phe Val Ser Val Gly Leu Pro Asn Met Thr Gln Gly Phe Trp 
        115                 120                 125             


Glu Asn Ser Met Leu Thr Asp Pro Gly Asn Val Gln Lys Ala Val Cys 
    130                 135                 140                 


His Pro Thr Ala Trp Asp Leu Gly Lys Gly Asp Phe Arg Ile Leu Met 
145                 150                 155                 160 


Cys Thr Lys Val Thr Met Asp Asp Phe Leu Thr Ala His His Glu Met 
                165                 170                 175     


Gly His Ile Gln Tyr Asp Met Ala Tyr Ala Ala Gln Pro Phe Leu Leu 
            180                 185                 190         


Arg Asn Gly Ala Asn Glu Gly Phe His Glu Ala Val Gly Glu Ile Met 
        195                 200                 205             


Ser Leu Ser Ala Ala Thr Pro Lys His Leu Lys Ser Ile Gly Leu Leu 
    210                 215                 220                 


Ser 
225 


<210>  26
<211>  225
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Fragment combination of Peptidase domain (19-103; 301-425) of 
       ACE-2 with substitution Glu375Gln

<400>  26

Ser Thr Ile Glu Glu Gln Ala Lys Thr Phe Leu Asp Lys Phe Asn His 
1               5                   10                  15      


Glu Ala Glu Asp Leu Phe Tyr Gln Ser Ser Leu Ala Ser Trp Asn Tyr 
            20                  25                  30          


Asn Thr Asn Ile Thr Glu Glu Asn Val Gln Asn Met Asn Asn Ala Gly 
        35                  40                  45              


Asp Lys Trp Ser Ala Phe Leu Lys Glu Gln Ser Thr Leu Ala Gln Met 
    50                  55                  60                  


Tyr Pro Leu Gln Glu Ile Gln Asn Leu Thr Val Lys Leu Gln Leu Gln 
65                  70                  75                  80  


Ala Leu Gln Gln Asn Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly 
                85                  90                  95      


Gly Gly Gly Ser Ala Trp Asp Ala Gln Arg Ile Phe Lys Glu Ala Glu 
            100                 105                 110         


Lys Phe Phe Val Ser Val Gly Leu Pro Asn Met Thr Gln Gly Phe Trp 
        115                 120                 125             


Glu Asn Ser Met Leu Thr Asp Pro Gly Asn Val Gln Lys Ala Val Cys 
    130                 135                 140                 


His Pro Thr Ala Trp Asp Leu Gly Lys Gly Asp Phe Arg Ile Leu Met 
145                 150                 155                 160 


Cys Thr Lys Val Thr Met Asp Asp Phe Leu Thr Ala His His Gln Met 
                165                 170                 175     


Gly His Ile Gln Tyr Asp Met Ala Tyr Ala Ala Gln Pro Phe Leu Leu 
            180                 185                 190         


Arg Asn Gly Ala Asn Glu Gly Phe His Glu Ala Val Gly Glu Ile Met 
        195                 200                 205             


Ser Leu Ser Ala Ala Thr Pro Lys His Leu Lys Ser Ile Gly Leu Leu 
    210                 215                 220                 


Ser 
225 


<210>  27
<211>  238
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  (6)_ACE2_19-103;301-425_E375Q

<400>  27

Met Ser Thr Ile Glu Glu Gln Ala Lys Thr Phe Leu Asp Lys Phe Asn 
1               5                   10                  15      


His Glu Ala Glu Asp Leu Phe Tyr Gln Ser Ser Leu Ala Ser Trp Asn 
            20                  25                  30          


Tyr Asn Thr Asn Ile Thr Glu Glu Asn Val Gln Asn Met Asn Asn Ala 
        35                  40                  45              


Gly Asp Lys Trp Ser Ala Phe Leu Lys Glu Gln Ser Thr Leu Ala Gln 
    50                  55                  60                  


Met Tyr Pro Leu Gln Glu Ile Gln Asn Leu Thr Val Lys Leu Gln Leu 
65                  70                  75                  80  


Gln Ala Leu Gln Gln Asn Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
                85                  90                  95      


Gly Gly Gly Gly Ser Ala Trp Asp Ala Gln Arg Ile Phe Lys Glu Ala 
            100                 105                 110         


Glu Lys Phe Phe Val Ser Val Gly Leu Pro Asn Met Thr Gln Gly Phe 
        115                 120                 125             


Trp Glu Asn Ser Met Leu Thr Asp Pro Gly Asn Val Gln Lys Ala Val 
    130                 135                 140                 


Cys His Pro Thr Ala Trp Asp Leu Gly Lys Gly Asp Phe Arg Ile Leu 
145                 150                 155                 160 


Met Cys Thr Lys Val Thr Met Asp Asp Phe Leu Thr Ala His His Gln 
                165                 170                 175     


Met Gly His Ile Gln Tyr Asp Met Ala Tyr Ala Ala Gln Pro Phe Leu 
            180                 185                 190         


Leu Arg Asn Gly Ala Asn Glu Gly Phe His Glu Ala Val Gly Glu Ile 
        195                 200                 205             


Met Ser Leu Ser Ala Ala Thr Pro Lys His Leu Lys Ser Ile Gly Leu 
    210                 215                 220                 


Leu Ser Gly Gly Gly Ser His His His His His His His His 
225                 230                 235             


<210>  28
<211>  125
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Fragment 301-425 of Peptidase domain (1-615) of human ACE-2

<400>  28

Ala Trp Asp Ala Gln Arg Ile Phe Lys Glu Ala Glu Lys Phe Phe Val 
1               5                   10                  15      


Ser Val Gly Leu Pro Asn Met Thr Gln Gly Phe Trp Glu Asn Ser Met 
            20                  25                  30          


Leu Thr Asp Pro Gly Asn Val Gln Lys Ala Val Cys His Pro Thr Ala 
        35                  40                  45              


Trp Asp Leu Gly Lys Gly Asp Phe Arg Ile Leu Met Cys Thr Lys Val 
    50                  55                  60                  


Thr Met Asp Asp Phe Leu Thr Ala His His Glu Met Gly His Ile Gln 
65                  70                  75                  80  


Tyr Asp Met Ala Tyr Ala Ala Gln Pro Phe Leu Leu Arg Asn Gly Ala 
                85                  90                  95      


Asn Glu Gly Phe His Glu Ala Val Gly Glu Ile Met Ser Leu Ser Ala 
            100                 105                 110         


Ala Thr Pro Lys His Leu Lys Ser Ile Gly Leu Leu Ser 
        115                 120                 125 


<210>  29
<211>  194
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Fragment combination of Peptidase domain (19-103; 342-425) of 
       ACE-2

<400>  29

Ser Thr Ile Glu Glu Gln Ala Lys Thr Phe Leu Asp Lys Phe Asn His 
1               5                   10                  15      


Glu Ala Glu Asp Leu Phe Tyr Gln Ser Ser Leu Ala Ser Trp Asn Tyr 
            20                  25                  30          


Asn Thr Asn Ile Thr Glu Glu Asn Val Gln Asn Met Asn Asn Ala Gly 
        35                  40                  45              


Asp Lys Trp Ser Ala Phe Leu Lys Glu Gln Ser Thr Leu Ala Gln Met 
    50                  55                  60                  


Tyr Pro Leu Gln Glu Ile Gln Asn Leu Thr Val Lys Leu Gln Leu Gln 
65                  70                  75                  80  


Ala Leu Gln Gln Asn Glu Ala Ala Ala Lys Glu Ala Ala Ala Lys Glu 
                85                  90                  95      


Ala Ala Ala Lys Glu Ala Ala Ala Lys Glu Ala Ala Ala Lys Ala Val 
            100                 105                 110         


Cys His Pro Thr Ala Trp Asp Leu Gly Lys Gly Asp Phe Arg Ile Leu 
        115                 120                 125             


Met Cys Thr Lys Val Thr Met Asp Asp Phe Leu Thr Ala His His Glu 
    130                 135                 140                 


Met Gly His Ile Gln Tyr Asp Met Ala Tyr Ala Ala Gln Pro Phe Leu 
145                 150                 155                 160 


Leu Arg Asn Gly Ala Asn Glu Gly Phe His Glu Ala Val Gly Glu Ile 
                165                 170                 175     


Met Ser Leu Ser Ala Ala Thr Pro Lys His Leu Lys Ser Ile Gly Leu 
            180                 185                 190         


Leu Ser 
        


<210>  30
<211>  194
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Fragment combination of Peptidase domain (19-103; 342-425) of 
       ACE-2 with substitution Glu375Gln

<400>  30

Ser Thr Ile Glu Glu Gln Ala Lys Thr Phe Leu Asp Lys Phe Asn His 
1               5                   10                  15      


Glu Ala Glu Asp Leu Phe Tyr Gln Ser Ser Leu Ala Ser Trp Asn Tyr 
            20                  25                  30          


Asn Thr Asn Ile Thr Glu Glu Asn Val Gln Asn Met Asn Asn Ala Gly 
        35                  40                  45              


Asp Lys Trp Ser Ala Phe Leu Lys Glu Gln Ser Thr Leu Ala Gln Met 
    50                  55                  60                  


Tyr Pro Leu Gln Glu Ile Gln Asn Leu Thr Val Lys Leu Gln Leu Gln 
65                  70                  75                  80  


Ala Leu Gln Gln Asn Glu Ala Ala Ala Lys Glu Ala Ala Ala Lys Glu 
                85                  90                  95      


Ala Ala Ala Lys Glu Ala Ala Ala Lys Glu Ala Ala Ala Lys Ala Val 
            100                 105                 110         


Cys His Pro Thr Ala Trp Asp Leu Gly Lys Gly Asp Phe Arg Ile Leu 
        115                 120                 125             


Met Cys Thr Lys Val Thr Met Asp Asp Phe Leu Thr Ala His His Gln 
    130                 135                 140                 


Met Gly His Ile Gln Tyr Asp Met Ala Tyr Ala Ala Gln Pro Phe Leu 
145                 150                 155                 160 


Leu Arg Asn Gly Ala Asn Glu Gly Phe His Glu Ala Val Gly Glu Ile 
                165                 170                 175     


Met Ser Leu Ser Ala Ala Thr Pro Lys His Leu Lys Ser Ile Gly Leu 
            180                 185                 190         


Leu Ser 
        


<210>  31
<211>  207
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  (7)_ACE2_19-103;342-425_E375Q

<400>  31

Met Ser Thr Ile Glu Glu Gln Ala Lys Thr Phe Leu Asp Lys Phe Asn 
1               5                   10                  15      


His Glu Ala Glu Asp Leu Phe Tyr Gln Ser Ser Leu Ala Ser Trp Asn 
            20                  25                  30          


Tyr Asn Thr Asn Ile Thr Glu Glu Asn Val Gln Asn Met Asn Asn Ala 
        35                  40                  45              


Gly Asp Lys Trp Ser Ala Phe Leu Lys Glu Gln Ser Thr Leu Ala Gln 
    50                  55                  60                  


Met Tyr Pro Leu Gln Glu Ile Gln Asn Leu Thr Val Lys Leu Gln Leu 
65                  70                  75                  80  


Gln Ala Leu Gln Gln Asn Glu Ala Ala Ala Lys Glu Ala Ala Ala Lys 
                85                  90                  95      


Glu Ala Ala Ala Lys Glu Ala Ala Ala Lys Glu Ala Ala Ala Lys Ala 
            100                 105                 110         


Val Cys His Pro Thr Ala Trp Asp Leu Gly Lys Gly Asp Phe Arg Ile 
        115                 120                 125             


Leu Met Cys Thr Lys Val Thr Met Asp Asp Phe Leu Thr Ala His His 
    130                 135                 140                 


Gln Met Gly His Ile Gln Tyr Asp Met Ala Tyr Ala Ala Gln Pro Phe 
145                 150                 155                 160 


Leu Leu Arg Asn Gly Ala Asn Glu Gly Phe His Glu Ala Val Gly Glu 
                165                 170                 175     


Ile Met Ser Leu Ser Ala Ala Thr Pro Lys His Leu Lys Ser Ile Gly 
            180                 185                 190         


Leu Leu Ser Gly Gly Gly Ser His His His His His His His His 
        195                 200                 205         


<210>  32
<211>  84
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Fragment 342-425 of Peptidase domain (1-615) of human ACE-2

<400>  32

Ala Val Cys His Pro Thr Ala Trp Asp Leu Gly Lys Gly Asp Phe Arg 
1               5                   10                  15      


Ile Leu Met Cys Thr Lys Val Thr Met Asp Asp Phe Leu Thr Ala His 
            20                  25                  30          


His Glu Met Gly His Ile Gln Tyr Asp Met Ala Tyr Ala Ala Gln Pro 
        35                  40                  45              


Phe Leu Leu Arg Asn Gly Ala Asn Glu Gly Phe His Glu Ala Val Gly 
    50                  55                  60                  


Glu Ile Met Ser Leu Ser Ala Ala Thr Pro Lys His Leu Lys Ser Ile 
65                  70                  75                  80  


Gly Leu Leu Ser 
                


<210>  33
<211>  25
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Linker 3

<400>  33

Glu Ala Ala Ala Lys Glu Ala Ala Ala Lys Glu Ala Ala Ala Lys Glu 
1               5                   10                  15      


Ala Ala Ala Lys Glu Ala Ala Ala Lys 
            20                  25  


<210>  34
<211>  33
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acids 21-53 of human ACE-2

<400>  34

Ile Glu Glu Gln Ala Lys Thr Phe Leu Asp Lys Phe Asn His Glu Ala 
1               5                   10                  15      


Glu Asp Leu Phe Tyr Gln Ser Ser Leu Ala Ser Trp Asn Tyr Asn Thr 
            20                  25                  30          


Asn 
    


<210>  35
<211>  25
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acids 56-80 of human ACE-2

<400>  35

Glu Glu Asn Val Gln Asn Met Asn Asn Ala Gly Asp Lys Trp Ser Ala 
1               5                   10                  15      


Phe Leu Lys Glu Gln Ser Thr Leu Ala 
            20                  25  


<210>  36
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acids 91-100 of human ACE-2

<400>  36

Leu Thr Val Lys Leu Gln Leu Gln Ala Leu 
1               5                   10  


<210>  37
<211>  20
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acids 110-129 of human ACE-2

<400>  37

Glu Asp Lys Ser Lys Arg Leu Asn Thr Ile Leu Asn Thr Met Ser Thr 
1               5                   10                  15      


Ile Tyr Ser Thr 
            20  


<210>  38
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acids 294-300

<400>  38

Thr Asp Ala Met Val Asp Gln 
1               5           


<210>  39
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acids 304-318 of human ACE-2

<400>  39

Ala Gln Arg Ile Phe Lys Glu Ala Glu Lys Phe Phe Val Ser Val 
1               5                   10                  15  


<210>  40
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acids 325-330 of human ACE-2

<400>  40

Gln Gly Phe Trp Glu Asn 
1               5       


<210>  41
<211>  20
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acids 366-385 of human ACE-2

<400>  41

Met Asp Asp Phe Leu Thr Ala His His Glu Met Gly His Ile Gln Tyr 
1               5                   10                  15      


Asp Met Ala Tyr 
            20  


<210>  42
<211>  13
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acids 400-412 of human ACE-2

<400>  42

Phe His Glu Ala Val Gly Glu Ile Met Ser Leu Ser Ala 
1               5                   10              


<210>  43
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acids 415-421 of human ACE-2

<400>  43

Pro Lys His Leu Lys Ser Ile 
1               5           


