                         SEQUENCE LISTING

<110>  Deutsches Krebsforschungszentrum
 
<120>  Polynucleotide detection by Cas nucleases

<130>  DK16159PC

<150>  EP20173912.5
<151>  2020-05-11

<150>  EP20190953.8
<151>  2020-08-13

<160>  38    

<170>  PatentIn version 3.5

<210>  1
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  S-RPA-Forward

<400>  1
tcctaggttg aagataaccc acataataag                                        30


<210>  2
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  S-RPA-Reverse

<400>  2
tcctaggttg aagataaccc acataataag                                        30


<210>  3
<211>  55
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Orf1ab-RPA-Forward

<400>  3
gaaattaata cgactcacta tagggcgaag ttgtaggaga cattatactt aaacc            55


<210>  4
<211>  31
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Orf1ab-RPA-Reverse

<400>  4
tagtaagact agaattgtct acataagcag c                                      31


<210>  5
<211>  64
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  S-crRNA

<400>  5
gauuuagacu accccaaaaa cgaaggggac uaaaacgcag caccagcugu ccaaccugaa       60

gaag                                                                    64


<210>  6
<211>  64
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Orf1ab-crRNA

<400>  6
gauuuagacu accccaaaaa cgaaggggac uaaaacccaa ccucuucugu aauuuuuaaa       60

cuau                                                                    64


<210>  7
<211>  14
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  lateral flow reporter RNA


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  2'-O-methyl-adenosine

<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  6-carboxyfluorescein

<220>
<221>  misc_feature
<222>  (7)..(7)
<223>  2'-O-methyl-adenosine

<220>
<221>  misc_feature
<222>  (8)..(8)
<223>  2'-O-methyl-adenosine

<220>
<221>  misc_feature
<222>  (14)..(14)
<223>  biotin

<220>
<221>  misc_feature
<222>  (14)..(14)
<223>  2'-O-methyl-adenosine

<400>  7
aauggcaaau ggca                                                         14


<210>  8
<211>  56
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  N-RPA-Forward_v1

<400>  8
gaaattaata cgactcacta tagggtaatc agacaaggaa ctgattacaa acattg           56


<210>  9
<211>  64
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  N-RPA-Reverse_v1

<400>  9
gacttccatg ccaatgcgcg acattccgaa gagacttcca tgccaatgcg cgacattccg       60

aaga                                                                    64


<210>  10
<211>  56
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  N-RPA-Forward_v2

<400>  10
gaaattaata cgactcacta tagggactaa tcagacaagg aactgattac aaacat           56


<210>  11
<211>  32
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  N-RPA-Reverse_v2

<400>  11
cacgttcccg aaggtgtgac ttccatgcca at                                     32


<210>  12
<211>  58
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  E-RPA-Forward_v1

<400>  12
gaaattaata cgactcacta taggggttaa tagttaatag cgtacttctt tttcttgc         58


<210>  13
<211>  31
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  E-RPA-Reverse_v1

<400>  13
ttaacaatat tgcagcagta cgcacacaat c                                      31


<210>  14
<211>  58
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  E-RPA-Forward_v2

<400>  14
gaaattaata cgactcacta tagggatagt taatagcgta cttctttttc ttgctttc         58


<210>  15
<211>  31
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  E-RPA-Reverse_v2

<400>  15
tacaagactc acgttaacaa tattgcagca g                                      31


<210>  16
<211>  64
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  N-crRNA

<400>  16
gauuuagacu accccaaaaa cgaaggggac uaaaacgaac gcugaagcgc ugggggcaaa       60

uugu                                                                    64


<210>  17
<211>  64
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  E-crRNA

<400>  17
gauuuagacu accccaaaaa cgaaggggac uaaaacucga agcgcaguaa ggauggcuag       60

ugua                                                                    64


<210>  18
<211>  11
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  reporter DNA sequence

<400>  18
ttattattat t                                                            11


<210>  19
<211>  11
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  fluorescence reporter DNA


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  6-carboxyfluorescein

<220>
<221>  misc_feature
<222>  (11)..(11)
<223>  Iowa Black FQ

<400>  19
ttattattat t                                                            11


<210>  20
<211>  11
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  lateral flow reporter DNA -1


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  6-carboxyfluorescein

<220>
<221>  misc_feature
<222>  (11)..(11)
<223>  biotin

<400>  20
ttattattat t                                                            11


<210>  21
<211>  11
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  lateral flow reporter DNA - 2


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  6-carboxyfluorescein

<220>
<221>  misc_feature
<222>  (11)..(11)
<223>  digoxigenin

<400>  21
ttattattat t                                                            11


<210>  22
<211>  11
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  lateral flow reporter DNA - 3


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  digoxigenin

<220>
<221>  misc_feature
<222>  (11)..(11)
<223>  biotin

<400>  22
ttattattat t                                                            11


<210>  23
<211>  41
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Type V crRNA (Example 3)

<400>  23
uaauuucuac uaaguguaga ugaccugcga gcggguucug a                           41


<210>  24
<211>  6
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  lateral flow reporter RNA-2


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  6-carboxyfluorescein

<220>
<221>  misc_feature
<222>  (6)..(6)
<223>  biotin

<400>  24
uuuuuu                                                                   6


<210>  25
<211>  6
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  fluorescence reporter RNA


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  TEX615

<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  deoxyribonucleotide

<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  phosphorothioate bond to the following nucleotide

<220>
<221>  misc_feature
<222>  (2)..(2)
<223>  phosphorothioate bond to the following nucleotide

<220>
<221>  misc_feature
<222>  (2)..(2)
<223>  deoxyribonucleotide

<220>
<221>  misc_feature
<222>  (3)..(3)
<223>  ribonucleotide

<220>
<221>  misc_feature
<222>  (4)..(4)
<223>  ribonucleotide

<220>
<221>  misc_feature
<222>  (5)..(5)
<223>  deoxyribonucleotide

<220>
<221>  misc_feature
<222>  (6)..(6)
<223>  phosphorothioate bond to the following nucleotide

<220>
<221>  misc_feature
<222>  (6)..(6)
<223>  deoxyribonucleotide

<220>
<221>  misc_feature
<222>  (6)..(6)
<223>  Iowa Black RQ-Sp

<400>  25
taaugc                                                                   6


<210>  26
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  SARS-CoV (Wuhan) spike polypeptide partial sequence (Fig. 16)

<400>  26

Ala Ile His Val Ser 
1               5   


<210>  27
<211>  15
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  SARS-CoV (Wuhan) spike polypeptide encoding partial sequence 
       (Fig. 16)

<400>  27
gcuauacaug ucucu                                                        15


<210>  28
<211>  3
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  SARS-CoV2 (B.1.1.7) spike polypeptide partial sequence (Fig. 16)

<400>  28

Ala Ile Ser 
1           


<210>  29
<211>  9
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  SARS-CoV2 (B.1.1.7) spike polypeptide encoding partial sequence 
       (Fig. 16)

<400>  29
gcuaucucu                                                                9


<210>  30
<211>  63
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  crRNA HV69-70

<400>  30
gauuuagacu accccaaaaa cgaaggggac uaaacuuggu cccagagaca uguauagcau       60

gga                                                                     63


<210>  31
<211>  63
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  crRNA delHV69-70

<400>  31
gauuuagacu accccaaaaa cgaaggggac uaaacccauu ggucccagag auagcaugga       60

acc                                                                     63


<210>  32
<211>  39
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  crRNA HV69-70 coding sequence Fig. 16

<400>  32
acuugguucc augcuauaca ugucucuggg accaauggu                              39


<210>  33
<211>  39
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  crRNA delHV69-70 coding sequence Fig. 16

<400>  33
acuugguucc augcuaucuc ugggaccaau gguacuaag                              39


<210>  34
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Wuhan upper sequence Fig. 16

<400>  34
aggtttcaaa ctttacttgc tttacataga                                        30


<210>  35
<211>  36
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Wuhan lower sequence Fig. 16

<400>  35
acttctatgt aaagcaagta aagtttgaaa cctagt                                 36


<210>  36
<211>  12
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Wuhan Fig. 16

<400>  36

Thr Arg Phe Gln Thr Leu Leu Ala Leu His Arg Ser 
1               5                   10          


<210>  37
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Sample 12 lower sequence Fig. 16

<400>  37
acttctatgt aaagtttgaa acctagt                                           27


<210>  38
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Sample 11 Fig. 16

<400>  38
acttatatgt aaagtttgaa acctagt                                           27


