               SEQUENCE LISTING

<110> U. Windmöller Innovation GmbH & Co. KG
      Universität Bielefeld

<120> Verfahren zur Herstellung von hydroxyfettsäurebasierten Polyolen

<130> AD1496 PCT

<150> 20 17 4135.2
<151> 2020-05-12

<160> 3

<170> BiSSAP 1.3.6

<210> 1
<211> 2022
<212> DNA
<213> Artificial Sequence


<220> 
<223> Sn-Ohy Rohextrakt

<400> 1
atgggcagca gccatcatca tcatcatcac agcagcggcc tggtgccgcg cggcagccat      60

atggaagaag tgagttatcc caaagctgga ccgagcattg aagcgaacgt aggggatggg     120

cactggcgaa aggggccctc ggatacgctg ccgcctccgg acactgttgg accctatatg     180

cgcaaccgcc ccctgcctgt ggatcaagtg gaaggcagga aagcatggat catcggaagt     240

ggaatcgcgg gtctggcctc tgccttttac ttgatccgcg acgggcggat gaaggggcag     300

gacataacca tcctcgatgc cgtgggcact ccaggcggat cactggacgg ctcagggaac     360

gccgaagatg gctacctgat ccgaggcggg cgcgagatga actggaacta cgatcacttt     420

tgggatctct tccaggacat tcccgcgctg gagtacccgt ccccttactc ggtcttggat     480

gagtatcggg cggtgaacga caatgatcct aattggtcca agtcccgatt gatgcacaag     540

caaggccaaa ttcgggattt cagcaccttg gggctttctt ccgcccacca atgggaattg     600

atcaagcttc tcctgaagcg caaggaggac ctcgatgaca tcaccatcga acagtacttc     660

agcgatagct ttctggagac caacttctgg tacctctggc gctcgatgtt tgcgttccag     720

aactggcaaa gtctgctgga agtgaagttg tacatgcatc gctttttgga tgcaatcgac     780

ggcttgacgg atatgtcagc gctcgtgttc ccaaaataca accagtacga cagcttcgtc     840

gtccccctgg tcaactacct caagggccaa ggcgtcaacg tagaattcgg cacgcgcgtc     900

tacgacctgg acatgacgga caacaacggc gagcgtaccg tgacctccat tcttgcgaag     960

gtagacgggc gggatcagaa gattgacatc ggcgcgaagg acgtggtttt tgccctgact    1020

ggatcgatga cggagggtac agcctacggc gatctggata ctgctcccga cctcactcga    1080

gccaccacgc cccctggcga ctcaagcgat tgggcgttgt ggcagaacct ggccaagaag    1140

tcccacgtct ttggtaagcc tgaaaagttc tgcgggcaac ccagtcgctc gatgtgggag    1200

tctgccaccc tgacgtgcaa gccttcgccg ttgaccgagc gcctcaaaga tctctcaatc    1260

aatgaccctt attcgggaaa aacggtgacc ggtggaatca tcacctttac cgactcgaac    1320

tgggttctca gcttcacctg caatcgtcaa ccgcatttcc ccacacaacc agacgacgta    1380

ctggtgcttt gggtctatgc cttggtcatg gacagcaaag gcaaccatgt actaaaacca    1440

atgcctgagt gtacgggccg cgaaattctt gctgagcttt gctaccacct cggcattgtg    1500

gatcaggtgg atgaagtggc cagacagacc aaggttcgcc ttgccctgat gccattcatc    1560

acggctcaat ttatgccacg agctgctgga gatcgaccgc gtgttgttcc agccgggtgc    1620

accaatctcg ctctgctggg ccaattcgtg gagacgtcta atgacatcat cttcaccatg    1680

gagagttccg tcaggactgc gcggattggc gtgtacacgc ttctggggct acgaaagcag    1740

gtcgccgata tcagccccac gcaatacgac gtccgaaatc tgatcaaggg tgctcgtgcc    1800

ctgaacaaca acgagccgtt catgggcgag cggctgctcc atcgactgct cgacaacacc    1860

tacttcgccc acatcctccc gccgctgcca gcaggagacg gtggatccag cgatcaagcg    1920

gcaagctcgc gtatgaaggc caaccacact gcggcggcgg cacttggagc ggtgtctgat    1980

tggatccatc atgttcggga taaactgaag ccgggcgcct ga                       2022


<210> 2
<211> 673
<212> PRT
<213> Artificial Sequence


<220> 
<223> Sn-Ohy Rohextrakt

<400> 2
Met Gly Ser Ser His His His His His His Ser Ser Gly Leu Val Pro 
1               5                   10                  15      
Arg Gly Ser His Met Glu Glu Val Ser Tyr Pro Lys Ala Gly Pro Ser 
            20                  25                  30          
Ile Glu Ala Asn Val Gly Asp Gly His Trp Arg Lys Gly Pro Ser Asp 
        35                  40                  45              
Thr Leu Pro Pro Pro Asp Thr Val Gly Pro Tyr Met Arg Asn Arg Pro 
    50                  55                  60                  
Leu Pro Val Asp Gln Val Glu Gly Arg Lys Ala Trp Ile Ile Gly Ser 
65                  70                  75                  80  
Gly Ile Ala Gly Leu Ala Ser Ala Phe Tyr Leu Ile Arg Asp Gly Arg 
                85                  90                  95      
Met Lys Gly Gln Asp Ile Thr Ile Leu Asp Ala Val Gly Thr Pro Gly 
            100                 105                 110         
Gly Ser Leu Asp Gly Ser Gly Asn Ala Glu Asp Gly Tyr Leu Ile Arg 
        115                 120                 125             
Gly Gly Arg Glu Met Asn Trp Asn Tyr Asp His Phe Trp Asp Leu Phe 
    130                 135                 140                 
Gln Asp Ile Pro Ala Leu Glu Tyr Pro Ser Pro Tyr Ser Val Leu Asp 
145                 150                 155                 160 
Glu Tyr Arg Ala Val Asn Asp Asn Asp Pro Asn Trp Ser Lys Ser Arg 
                165                 170                 175     
Leu Met His Lys Gln Gly Gln Ile Arg Asp Phe Ser Thr Leu Gly Leu 
            180                 185                 190         
Ser Ser Ala His Gln Trp Glu Leu Ile Lys Leu Leu Leu Lys Arg Lys 
        195                 200                 205             
Glu Asp Leu Asp Asp Ile Thr Ile Glu Gln Tyr Phe Ser Asp Ser Phe 
    210                 215                 220                 
Leu Glu Thr Asn Phe Trp Tyr Leu Trp Arg Ser Met Phe Ala Phe Gln 
225                 230                 235                 240 
Asn Trp Gln Ser Leu Leu Glu Val Lys Leu Tyr Met His Arg Phe Leu 
                245                 250                 255     
Asp Ala Ile Asp Gly Leu Thr Asp Met Ser Ala Leu Val Phe Pro Lys 
            260                 265                 270         
Tyr Asn Gln Tyr Asp Ser Phe Val Val Pro Leu Val Asn Tyr Leu Lys 
        275                 280                 285             
Gly Gln Gly Val Asn Val Glu Phe Gly Thr Arg Val Tyr Asp Leu Asp 
    290                 295                 300                 
Met Thr Asp Asn Asn Gly Glu Arg Thr Val Thr Ser Ile Leu Ala Lys 
305                 310                 315                 320 
Val Asp Gly Arg Asp Gln Lys Ile Asp Ile Gly Ala Lys Asp Val Val 
                325                 330                 335     
Phe Ala Leu Thr Gly Ser Met Thr Glu Gly Thr Ala Tyr Gly Asp Leu 
            340                 345                 350         
Asp Thr Ala Pro Asp Leu Thr Arg Ala Thr Thr Pro Pro Gly Asp Ser 
        355                 360                 365             
Ser Asp Trp Ala Leu Trp Gln Asn Leu Ala Lys Lys Ser His Val Phe 
    370                 375                 380                 
Gly Lys Pro Glu Lys Phe Cys Gly Gln Pro Ser Arg Ser Met Trp Glu 
385                 390                 395                 400 
Ser Ala Thr Leu Thr Cys Lys Pro Ser Pro Leu Thr Glu Arg Leu Lys 
                405                 410                 415     
Asp Leu Ser Ile Asn Asp Pro Tyr Ser Gly Lys Thr Val Thr Gly Gly 
            420                 425                 430         
Ile Ile Thr Phe Thr Asp Ser Asn Trp Val Leu Ser Phe Thr Cys Asn 
        435                 440                 445             
Arg Gln Pro His Phe Pro Thr Gln Pro Asp Asp Val Leu Val Leu Trp 
    450                 455                 460                 
Val Tyr Ala Leu Val Met Asp Ser Lys Gly Asn His Val Leu Lys Pro 
465                 470                 475                 480 
Met Pro Glu Cys Thr Gly Arg Glu Ile Leu Ala Glu Leu Cys Tyr His 
                485                 490                 495     
Leu Gly Ile Val Asp Gln Val Asp Glu Val Ala Arg Gln Thr Lys Val 
            500                 505                 510         
Arg Leu Ala Leu Met Pro Phe Ile Thr Ala Gln Phe Met Pro Arg Ala 
        515                 520                 525             
Ala Gly Asp Arg Pro Arg Val Val Pro Ala Gly Cys Thr Asn Leu Ala 
    530                 535                 540                 
Leu Leu Gly Gln Phe Val Glu Thr Ser Asn Asp Ile Ile Phe Thr Met 
545                 550                 555                 560 
Glu Ser Ser Val Arg Thr Ala Arg Ile Gly Val Tyr Thr Leu Leu Gly 
                565                 570                 575     
Leu Arg Lys Gln Val Ala Asp Ile Ser Pro Thr Gln Tyr Asp Val Arg 
            580                 585                 590         
Asn Leu Ile Lys Gly Ala Arg Ala Leu Asn Asn Asn Glu Pro Phe Met 
        595                 600                 605             
Gly Glu Arg Leu Leu His Arg Leu Leu Asp Asn Thr Tyr Phe Ala His 
    610                 615                 620                 
Ile Leu Pro Pro Leu Pro Ala Gly Asp Gly Gly Ser Ser Asp Gln Ala 
625                 630                 635                 640 
Ala Ser Ser Arg Met Lys Ala Asn His Thr Ala Ala Ala Ala Leu Gly 
                645                 650                 655     
Ala Val Ser Asp Trp Ile His His Val Arg Asp Lys Leu Lys Pro Gly 
            660                 665                 670         
Ala 
    

<210> 3
<211> 60
<212> DNA
<213> Artificial Sequence


<220> 
<223> N-terminaler His6-Tag (pET28-Sn-Ohy(N)His6)

<400> 3
atgggcagca gccatcatca tcatcatcac agcagcggcc tggtgccgcg cggcagccat     60


