                         SEQUENCE LISTING

<110>  OP2LYSIS
 
<120>  SLOW RELEASE PLASMINOGEN ACTIVATOR FORMULATION FOR USE IN THE 
       TREATMENT OF THROMBOTIC OR HAEMORRHAGIC DISEASE

<130>  BCT210117 QT

<150>  EP20305462.2
<151>  2020-05-11

<160>  1     

<170>  PatentIn version 3.5

<210>  1
<211>  527
<212>  PRT
<213>  Artificial sequence

<220>
<223>  W253R/R275S tPA

<400>  1

Ser Tyr Gln Val Ile Cys Arg Asp Glu Lys Thr Gln Met Ile Tyr Gln 
1               5                   10                  15      


Gln His Gln Ser Trp Leu Arg Pro Val Leu Arg Ser Asn Arg Val Glu 
            20                  25                  30          


Tyr Cys Trp Cys Asn Ser Gly Arg Ala Gln Cys His Ser Val Pro Val 
        35                  40                  45              


Lys Ser Cys Ser Glu Pro Arg Cys Phe Asn Gly Gly Thr Cys Gln Gln 
    50                  55                  60                  


Ala Leu Tyr Phe Ser Asp Phe Val Cys Gln Cys Pro Glu Gly Phe Ala 
65                  70                  75                  80  


Gly Lys Cys Cys Glu Ile Asp Thr Arg Ala Thr Cys Tyr Glu Asp Gln 
                85                  90                  95      


Gly Ile Ser Tyr Arg Gly Thr Trp Ser Thr Ala Glu Ser Gly Ala Glu 
            100                 105                 110         


Cys Thr Asn Trp Asn Ser Ser Ala Leu Ala Gln Lys Pro Tyr Ser Gly 
        115                 120                 125             


Arg Arg Pro Asp Ala Ile Arg Leu Gly Leu Gly Asn His Asn Tyr Cys 
    130                 135                 140                 


Arg Asn Pro Asp Arg Asp Ser Lys Pro Trp Cys Tyr Val Phe Lys Ala 
145                 150                 155                 160 


Gly Lys Tyr Ser Ser Glu Phe Cys Ser Thr Pro Ala Cys Ser Glu Gly 
                165                 170                 175     


Asn Ser Asp Cys Tyr Phe Gly Asn Gly Ser Ala Tyr Arg Gly Thr His 
            180                 185                 190         


Ser Leu Thr Glu Ser Gly Ala Ser Cys Leu Pro Trp Asn Ser Met Ile 
        195                 200                 205             


Leu Ile Gly Lys Val Tyr Thr Ala Gln Asn Pro Ser Ala Gln Ala Leu 
    210                 215                 220                 


Gly Leu Gly Lys His Asn Tyr Cys Arg Asn Pro Asp Gly Asp Ala Lys 
225                 230                 235                 240 


Pro Trp Cys His Val Leu Lys Asn Arg Arg Leu Thr Arg Glu Tyr Cys 
                245                 250                 255     


Asp Val Pro Ser Cys Ser Thr Cys Gly Leu Arg Gln Tyr Ser Gln Pro 
            260                 265                 270         


Gln Phe Ser Ile Lys Gly Gly Leu Phe Ala Asp Ile Ala Ser His Pro 
        275                 280                 285             


Trp Gln Ala Ala Ile Phe Ala Lys His Arg Arg Ser Pro Gly Glu Arg 
    290                 295                 300                 


Phe Leu Cys Gly Gly Ile Leu Ile Ser Ser Cys Trp Ile Leu Ser Ala 
305                 310                 315                 320 


Ala His Cys Phe Gln Glu Arg Phe Pro Pro His His Leu Thr Val Ile 
                325                 330                 335     


Leu Gly Arg Thr Tyr Arg Val Val Pro Gly Glu Glu Glu Gln Lys Phe 
            340                 345                 350         


Glu Val Glu Lys Tyr Ile Val His Lys Glu Phe Asp Asp Asp Thr Tyr 
        355                 360                 365             


Asp Asn Asp Ile Ala Leu Leu Gln Leu Lys Ser Asp Ser Ser Arg Cys 
    370                 375                 380                 


Ala Gln Glu Ser Ser Val Val Arg Thr Val Cys Leu Pro Pro Ala Asp 
385                 390                 395                 400 


Leu Gln Leu Pro Asp Trp Thr Glu Cys Glu Leu Ser Gly Tyr Gly Lys 
                405                 410                 415     


His Glu Ala Leu Ser Pro Phe Tyr Ser Glu Arg Leu Lys Glu Ala His 
            420                 425                 430         


Val Arg Leu Tyr Pro Ser Ser Arg Cys Thr Ser Gln His Leu Leu Asn 
        435                 440                 445             


Arg Thr Val Thr Asp Asn Met Leu Cys Ala Gly Asp Thr Arg Ser Gly 
    450                 455                 460                 


Gly Pro Gln Ala Asn Leu His Asp Ala Cys Gln Gly Asp Ser Gly Gly 
465                 470                 475                 480 


Pro Leu Val Cys Leu Asn Asp Gly Arg Met Thr Leu Val Gly Ile Ile 
                485                 490                 495     


Ser Trp Gly Leu Gly Cys Gly Gln Lys Asp Val Pro Gly Val Tyr Thr 
            500                 505                 510         


Lys Val Thr Asn Tyr Leu Asp Trp Ile Arg Asp Asn Met Arg Pro 
        515                 520                 525         


