               SEQUENCE LISTING

<110> LeukoCom GmbH

<120> A novel antibody binding specifically to human CEACAM1/3/5 and use thereof

<130> P25629WO

<150> EP20173899.4
<151> 2020-05-11

<160> 21

<170> BiSSAP 1.3.6

<210> 1
<211> 8
<212> PRT
<213> Artificial Sequence


<220> 
<223> HCDR1

<400> 1
Gly Tyr Thr Phe Thr Thr Tyr Val 
1               5               

<210> 2
<211> 8
<212> PRT
<213> Artificial Sequence


<220> 
<223> HCDR2

<400> 2
Phe Asn Pro Tyr Asn Asp Gly Thr 
1               5               

<210> 3
<211> 11
<212> PRT
<213> Artificial Sequence


<220> 
<223> HCRD3

<400> 3
Ala Arg Trp Ala Tyr Asp Gly Ser Tyr Ala Tyr 
1               5                   10      

<210> 4
<211> 6
<212> PRT
<213> Artificial Sequence


<220> 
<223> LCDR1

<400> 4
Asp His Ile Asn Asn Trp 
1               5       

<210> 5
<211> 4
<212> PRT
<213> Artificial Sequence


<220> 
<223> LCDR2, wherein X at position 4 can be any amino acid

<400> 5
Gly Ala Thr Xaa 
1               

<210> 6
<211> 9
<212> PRT
<213> Artificial Sequence


<220> 
<223> LCDR3

<400> 6
Gln Gln Tyr Trp Arg Thr Pro Phe Thr 
1               5                   

<210> 7
<211> 118
<212> PRT
<213> Artificial Sequence


<220> 
<223> VH with HCDR1 to 3

<400> 7
Glu Val Gln Leu Gln Gln Ser Gly Pro Glu Leu Val Lys Pro Gly Thr 
1               5                   10                  15      
Ser Val Lys Met Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Thr Tyr 
            20                  25                  30          
Val Met His Trp Val Gln Gln Lys Pro Gly Gln Gly Leu Asp Trp Ile 
        35                  40                  45              
Gly Phe Phe Asn Pro Tyr Asn Asp Gly Thr Lys Tyr Asn Glu Lys Phe 
    50                  55                  60                  
Lys Gly Lys Ala Thr Leu Thr Ser Asp Lys Ser Ser Ser Thr Ala Tyr 
65                  70                  75                  80  
Met Glu Leu Ser Ser Leu Thr Ser Glu Asp Ser Ala Val Tyr Tyr Cys 
                85                  90                  95      
Ala Arg Trp Ala Tyr Asp Gly Ser Tyr Ala Tyr Trp Gly Gln Gly Thr 
            100                 105                 110         
Thr Leu Thr Val Ser Ser 
        115             

<210> 8
<211> 107
<212> PRT
<213> Artificial Sequence


<220> 
<223> VL with LCDR1 to 3

<400> 8
Asp Ile Gln Met Thr Gln Ser Ser Ser Tyr Leu Ser Val Phe Leu Gly 
1               5                   10                  15      
Gly Arg Val Thr Ile Thr Cys Lys Ala Ser Asp His Ile Asn Asn Trp 
            20                  25                  30          
Leu Ala Trp Tyr Gln Gln Lys Pro Gly Asn Ala Pro Arg Leu Leu Ile 
        35                  40                  45              
Ser Gly Ala Thr Ser Leu Glu Thr Gly Val Pro Ser Arg Phe Ser Gly 
    50                  55                  60                  
Ser Gly Ser Gly Lys Asp Tyr Thr Leu Ser Ile Ile Ser Leu Gln Thr 
65                  70                  75                  80  
Glu Asp Val Ala Thr Tyr Tyr Cys Gln Gln Tyr Trp Arg Thr Pro Phe 
                85                  90                  95      
Thr Phe Gly Ser Gly Thr Lys Leu Glu Ile Lys 
            100                 105         

<210> 9
<211> 354
<212> DNA
<213> Artificial Sequence


<220> 
<223> encoding VH

<400> 9
gaggtccagc tgcagcaatc tggacctgag ctggtaaagc ctgggacttc agtgaagatg      60

tcctgcaagg cttctggata cacattcact acctatgtta tgcactgggt gcaacagaag     120

cctgggcagg gccttgactg gattggattt tttaatcctt acaatgatgg tactaagtac     180

aatgagaagt tcaaaggcaa ggccacactg acttcagaca aatcctccag cacagcctac     240

atggaactca gcagcctgac ctctgaggac tctgcggtct attactgtgc aagatgggcc     300

tacgatggta gctacgccta ctggggccaa ggcaccactc tcacagtctc ctca           354


<210> 10
<211> 321
<212> DNA
<213> Artificial Sequence


<220> 
<223> encoding VL

<400> 10
gatatccaga tgacacaatc ttcatcctac ttgtctgtat ttctaggagg cagagtcacc      60

attacttgca aggcaagtga ccacattaat aattggttag cctggtatca gcagaaacca     120

ggaaatgctc ctaggctctt aatatctggg gcaaccagtt tggaaactgg ggttccttca     180

agattcagtg gcagtggatc tggaaaggat tacactctca gcattatcag tcttcagact     240

gaagatgttg ctacttatta ctgtcaacag tattggagaa ctccattcac gttcggctcg     300

gggacaaagt tggaaataaa a                                               321


<210> 11
<211> 10
<212> PRT
<213> Homo sapiens


<220> 
<223> fragment of huCEACAM1, epitope of antibody of the invention

<400> 11
Pro Gln Gln Leu Phe Gly Tyr Ser Trp Tyr 
1               5                   10  

<210> 12
<211> 526
<212> PRT
<213> Homo sapiens


<220> 
<223> CEACAM1

<400> 12
Met Gly His Leu Ser Ala Pro Leu His Arg Val Arg Val Pro Trp Gln 
1               5                   10                  15      
Gly Leu Leu Leu Thr Ala Ser Leu Leu Thr Phe Trp Asn Pro Pro Thr 
            20                  25                  30          
Thr Ala Gln Leu Thr Thr Glu Ser Met Pro Phe Asn Val Ala Glu Gly 
        35                  40                  45              
Lys Glu Val Leu Leu Leu Val His Asn Leu Pro Gln Gln Leu Phe Gly 
    50                  55                  60                  
Tyr Ser Trp Tyr Lys Gly Glu Arg Val Asp Gly Asn Arg Gln Ile Val 
65                  70                  75                  80  
Gly Tyr Ala Ile Gly Thr Gln Gln Ala Thr Pro Gly Pro Ala Asn Ser 
                85                  90                  95      
Gly Arg Glu Thr Ile Tyr Pro Asn Ala Ser Leu Leu Ile Gln Asn Val 
            100                 105                 110         
Thr Gln Asn Asp Thr Gly Phe Tyr Thr Leu Gln Val Ile Lys Ser Asp 
        115                 120                 125             
Leu Val Asn Glu Glu Ala Thr Gly Gln Phe His Val Tyr Pro Glu Leu 
    130                 135                 140                 
Pro Lys Pro Ser Ile Ser Ser Asn Asn Ser Asn Pro Val Glu Asp Lys 
145                 150                 155                 160 
Asp Ala Val Ala Phe Thr Cys Glu Pro Glu Thr Gln Asp Thr Thr Tyr 
                165                 170                 175     
Leu Trp Trp Ile Asn Asn Gln Ser Leu Pro Val Ser Pro Arg Leu Gln 
            180                 185                 190         
Leu Ser Asn Gly Asn Arg Thr Leu Thr Leu Leu Ser Val Thr Arg Asn 
        195                 200                 205             
Asp Thr Gly Pro Tyr Glu Cys Glu Ile Gln Asn Pro Val Ser Ala Asn 
    210                 215                 220                 
Arg Ser Asp Pro Val Thr Leu Asn Val Thr Tyr Gly Pro Asp Thr Pro 
225                 230                 235                 240 
Thr Ile Ser Pro Ser Asp Thr Tyr Tyr Arg Pro Gly Ala Asn Leu Ser 
                245                 250                 255     
Leu Ser Cys Tyr Ala Ala Ser Asn Pro Pro Ala Gln Tyr Ser Trp Leu 
            260                 265                 270         
Ile Asn Gly Thr Phe Gln Gln Ser Thr Gln Glu Leu Phe Ile Pro Asn 
        275                 280                 285             
Ile Thr Val Asn Asn Ser Gly Ser Tyr Thr Cys His Ala Asn Asn Ser 
    290                 295                 300                 
Val Thr Gly Cys Asn Arg Thr Thr Val Lys Thr Ile Ile Val Thr Glu 
305                 310                 315                 320 
Leu Ser Pro Val Val Ala Lys Pro Gln Ile Lys Ala Ser Lys Thr Thr 
                325                 330                 335     
Val Thr Gly Asp Lys Asp Ser Val Asn Leu Thr Cys Ser Thr Asn Asp 
            340                 345                 350         
Thr Gly Ile Ser Ile Arg Trp Phe Phe Lys Asn Gln Ser Leu Pro Ser 
        355                 360                 365             
Ser Glu Arg Met Lys Leu Ser Gln Gly Asn Thr Thr Leu Ser Ile Asn 
    370                 375                 380                 
Pro Val Lys Arg Glu Asp Ala Gly Thr Tyr Trp Cys Glu Val Phe Asn 
385                 390                 395                 400 
Pro Ile Ser Lys Asn Gln Ser Asp Pro Ile Met Leu Asn Val Asn Tyr 
                405                 410                 415     
Asn Ala Leu Pro Gln Glu Asn Gly Leu Ser Pro Gly Ala Ile Ala Gly 
            420                 425                 430         
Ile Val Ile Gly Val Val Ala Leu Val Ala Leu Ile Ala Val Ala Leu 
        435                 440                 445             
Ala Cys Phe Leu His Phe Gly Lys Thr Gly Arg Ala Ser Asp Gln Arg 
    450                 455                 460                 
Asp Leu Thr Glu His Lys Pro Ser Val Ser Asn His Thr Gln Asp His 
465                 470                 475                 480 
Ser Asn Asp Pro Pro Asn Lys Met Asn Glu Val Thr Tyr Ser Thr Leu 
                485                 490                 495     
Asn Phe Glu Ala Gln Gln Pro Thr Gln Pro Thr Ser Ala Ser Pro Ser 
            500                 505                 510         
Leu Thr Ala Thr Glu Ile Ile Tyr Ser Glu Val Lys Lys Gln 
        515                 520                 525     

<210> 13
<211> 12
<212> PRT
<213> Homo sapiens


<220> 
<223> S6

<400> 13
Lys Glu Val Leu Leu Leu Val His Asn Leu Pro Gln 
1               5                   10          

<210> 14
<211> 12
<212> PRT
<213> Homo sapiens


<220> 
<223> S7

<400> 14
Leu Leu Leu Val His Asn Leu Pro Gln Gln Leu Phe 
1               5                   10          

<210> 15
<211> 12
<212> PRT
<213> Homo sapiens


<220> 
<223> S8

<400> 15
Val His Asn Leu Pro Gln Gln Leu Phe Gly Tyr Ser 
1               5                   10          

<210> 16
<211> 12
<212> PRT
<213> Homo sapiens


<220> 
<223> S9

<400> 16
Leu Pro Gln Gln Leu Phe Gly Tyr Ser Trp Tyr Lys 
1               5                   10          

<210> 17
<211> 12
<212> PRT
<213> Homo sapiens


<220> 
<223> S10

<400> 17
Gln Leu Phe Gly Tyr Ser Trp Tyr Lys Gly Glu Arg 
1               5                   10          

<210> 18
<211> 12
<212> PRT
<213> Homo sapiens


<220> 
<223> S11

<400> 18
Gly Tyr Ser Trp Tyr Lys Gly Glu Arg Val Asp Gly 
1               5                   10          

<210> 19
<211> 12
<212> PRT
<213> Homo sapiens


<220> 
<223> S12

<400> 19
Trp Tyr Lys Gly Glu Arg Val Asp Gly Asn Arg Gln 
1               5                   10          

<210> 20
<211> 12
<212> PRT
<213> Homo sapiens


<220> 
<223> S13

<400> 20
Gly Glu Arg Val Asp Gly Asn Arg Gln Ile Val Gly 
1               5                   10          

<210> 21
<211> 12
<212> PRT
<213> Homo sapiens


<220> 
<223> S14

<400> 21
Val Asp Gly Asn Arg Gln Ile Val Gly Tyr Ala Ile 
1               5                   10          

