序列表

<110>  上海荣瑞医药科技有限公司

<120>  溶瘤病毒与免疫检查点抑制剂联合治疗肿瘤

<130>  0190-PA-009

<160>  13

<170>  PatentIn version 3.5

<210>  1
<211>  690
<212>  DNA
<213>  水疱性口炎病毒印第安纳株(VSV MuddSummer)

<400>  1

atgagttcct taaagaagat tctcggtctg aaggggaaag gtaagaaatc taagaaatta       60

gggatcgcac caccccctta tgaagaggac actagcatgg agtatgctcc gagcgctcca      120

attgacaaat cctattttgg agttgacgag atggacacct atgatccgaa tcaattaaga      180

tatgagaaat tcttctttac agtgaaaatg acggttagat ctaatcgtcc gttcagaaca      240

tactcagatg tggcagccgc tgtatcccat tgggatcaca tgtacatcgg aatggcaggg      300

aaacgtccct tctacaaaat cttggctttt ttgggttctt ctaatctaaa ggccactcca      360

gcggtattgg cagatcaagg tcaaccagag tatcacgctc actgcgaagg cagggcttat      420

ttgccacata ggatggggaa gacccctccc atgctcaatg taccagagca cttcagaaga      480

ccattcaata taggtcttta caagggaacg attgagctca caatgaccat ctacgatgat      540

gagtcactgg aagcagctcc tatgatctgg gatcatttca attcttccaa attttctgat      600

ttcagagaga aggccttaat gtttggcctg attgtcgaga aaaaggcatc tggagcgtgg      660

gtcctggact ctatcggcca cttcaaatga                                       690


<210>  2
<211>  229
<212>  PRT
<213> 水疱性口炎病毒印第安纳株(VSV MuddSummer)

<400>  2

Met Ser Ser Leu Lys Lys Ile Leu Gly Leu Lys Gly Lys Gly Lys Lys 
1               5                   10                  15      


Ser Lys Lys Leu Gly Ile Ala Pro Pro Pro Tyr Glu Glu Asp Thr Ser 
            20                  25                  30          


Met Glu Tyr Ala Pro Ser Ala Pro Ile Asp Lys Ser Tyr Phe Gly Val 
        35                  40                  45              


Asp Glu Met Asp Thr Tyr Asp Pro Asn Gln Leu Arg Tyr Glu Lys Phe 
    50                  55                  60                  


Phe Phe Thr Val Lys Met Thr Val Arg Ser Asn Arg Pro Phe Arg Thr 
65                  70                  75                  80  


Tyr Ser Asp Val Ala Ala Ala Val Ser His Trp Asp His Met Tyr Ile 
                85                  90                  95      


Gly Met Ala Gly Lys Arg Pro Phe Tyr Lys Ile Leu Ala Phe Leu Gly 
            100                 105                 110         


Ser Ser Asn Leu Lys Ala Thr Pro Ala Val Leu Ala Asp Gln Gly Gln 
        115                 120                 125             


Pro Glu Tyr His Ala His Cys Glu Gly Arg Ala Tyr Leu Pro His Arg 
    130                 135                 140                 


Met Gly Lys Thr Pro Pro Met Leu Asn Val Pro Glu His Phe Arg Arg 
145                 150                 155                 160 


Pro Phe Asn Ile Gly Leu Tyr Lys Gly Thr Ile Glu Leu Thr Met Thr 
                165                 170                 175     


Ile Tyr Asp Asp Glu Ser Leu Glu Ala Ala Pro Met Ile Trp Asp His 
            180                 185                 190         


Phe Asn Ser Ser Lys Phe Ser Asp Phe Arg Glu Lys Ala Leu Met Phe 
        195                 200                 205             


Gly Leu Ile Val Glu Lys Lys Ala Ser Gly Ala Trp Val Leu Asp Ser 
    210                 215                 220                 


Ile Ser His Phe Lys 
225                 


<210>  3
<211>  687
<212>  DNA
<213>  人工序列（Artificial Sequence）

<220>
<223>  基质蛋白M（M51R，ΔL111，V221F，S226R）

<400>  3
atgagttcct taaagaagat tctcggtctg aaggggaaag gtaagaaatc taagaaatta       60

gggatcgcac caccccctta tgaagaggac actagcatgg agtatgctcc gagcgctcca      120

attgacaaat cctattttgg agttgacgag atggacacct atgatccgaa tcaattaaga      180

tatgagaaat tcttctttac agtgaaaatg acggttagat ctaatcgtcc gttcagaaca      240

tactcagatg tggcagccgc tgtatcccat tgggatcaca tgtacatcgg aatggcaggg      300

aaacgtccct tctacaaaat cttggctttg ggttcttcta atctaaaggc cactccagcg      360

gtattggcag atcaaggtca accagagtat cacgctcact gcgaaggcag ggcttatttg      420

ccacatagga tggggaagac ccctcccatg ctcaatgtac cagagcactt cagaagacca      480

ttcaatatag gtctttacaa gggaacgatt gagctcacaa tgaccatcta cgatgatgag      540

tcactggaag cagctcctat gatctgggat catttcaatt cttccaaatt ttctgatttc      600

agagagaagg ccttaatgtt tggcctgatt gtcgagaaaa aggcatctgg agcgtgggtc      660

ctggactcta tcggccactt caaatga                                          687


<210>  4
<211>  228
<212>  PRT
<213>  人工序列（Artificial Sequence）

<220>
<223>  基质蛋白M（M51R，ΔL111，V221F，S226R）（PRT）

<400>  4

Met Ser Ser Leu Lys Lys Ile Leu Gly Leu Lys Gly Lys Gly Lys Lys 
1               5                   10                  15      


Ser Lys Lys Leu Gly Ile Ala Pro Pro Pro Tyr Glu Glu Asp Thr Ser 
            20                  25                  30          


Met Glu Tyr Ala Pro Ser Ala Pro Ile Asp Lys Ser Tyr Phe Gly Val 
        35                  40                  45              


Asp Glu Arg Asp Thr Tyr Asp Pro Asn Gln Leu Arg Tyr Glu Lys Phe 
    50                  55                  60                  


Phe Phe Thr Val Lys Met Thr Val Arg Ser Asn Arg Pro Phe Arg Thr 
65                  70                  75                  80  


Tyr Ser Asp Val Ala Ala Ala Val Ser His Trp Asp His Met Tyr Ile 
                85                  90                  95      


Gly Met Ala Gly Lys Arg Pro Phe Tyr Lys Ile Leu Ala Phe Gly Ser 
            100                 105                 110         


Ser Asn Leu Lys Ala Thr Pro Ala Val Leu Ala Asp Gln Gly Gln Pro 
        115                 120                 125             


Glu Tyr His Ala His Cys Glu Gly Arg Ala Tyr Leu Pro His Arg Met 
    130                 135                 140                 


Gly Lys Thr Pro Pro Met Leu Asn Val Pro Glu His Phe Arg Arg Pro 
145                 150                 155                 160 


Phe Asn Ile Gly Leu Tyr Lys Gly Thr Ile Glu Leu Thr Met Thr Ile 
                165                 170                 175     


Tyr Asp Asp Glu Ser Leu Glu Ala Ala Pro Met Ile Trp Asp His Phe 
            180                 185                 190         


Asn Ser Ser Lys Phe Ser Asp Phe Arg Glu Lys Ala Leu Met Phe Gly 
        195                 200                 205             


Leu Ile Val Glu Lys Lys Ala Ser Gly Ala Trp Phe Leu Asp Ser Ile 
    210                 215                 220                 


Arg His Phe Lys 
225             


<210>  5
<211>  20
<212>  DNA
<213>  人工序列（Artificial Sequence）

<220>
<223>  引物1

<400>  5
aaaaaagtaa cagatatcac                                                   20


<210>  6
<211>  24
<212>  DNA
<213>  人工序列（Artificial Sequence）

<220>
<223>  引物2

<400>  6
acatttttcc agtttccttt ttgg                                              24


<210>  7
<211>  228
<212>  PRT
<213>  人工序列（Artificial Sequence）

<220>
<223>  基质蛋白M（ΔL111）

<400>  7

Met Ser Ser Leu Lys Lys Ile Leu Gly Leu Lys Gly Lys Gly Lys Lys 
1               5                   10                  15      


Ser Lys Lys Leu Gly Ile Ala Pro Pro Pro Tyr Glu Glu Asp Thr Ser 
            20                  25                  30          


Met Glu Tyr Ala Pro Ser Ala Pro Ile Asp Lys Ser Tyr Phe Gly Val 
        35                  40                  45              


Asp Glu Met Asp Thr Tyr Asp Pro Asn Gln Leu Arg Tyr Glu Lys Phe 
    50                  55                  60                  


Phe Phe Thr Val Lys Met Thr Val Arg Ser Asn Arg Pro Phe Arg Thr 
65                  70                  75                  80  


Tyr Ser Asp Val Ala Ala Ala Val Ser His Trp Asp His Met Tyr Ile 
                85                  90                  95      


Gly Met Ala Gly Lys Arg Pro Phe Tyr Lys Ile Leu Ala Phe Gly Ser 
            100                 105                 110         


Ser Asn Leu Lys Ala Thr Pro Ala Val Leu Ala Asp Gln Gly Gln Pro 
        115                 120                 125             


Glu Tyr His Ala His Cys Glu Gly Arg Ala Tyr Leu Pro His Arg Met 
    130                 135                 140                 


Gly Lys Thr Pro Pro Met Leu Asn Val Pro Glu His Phe Arg Arg Pro 
145                 150                 155                 160 


Phe Asn Ile Gly Leu Tyr Lys Gly Thr Ile Glu Leu Thr Met Thr Ile 
                165                 170                 175     


Tyr Asp Asp Glu Ser Leu Glu Ala Ala Pro Met Ile Trp Asp His Phe 
            180                 185                 190         


Asn Ser Ser Lys Phe Ser Asp Phe Arg Glu Lys Ala Leu Met Phe Gly 
        195                 200                 205             


Leu Ile Val Glu Lys Lys Ala Ser Gly Ala Trp Val Leu Asp Ser Ile 
    210                 215                 220                 


Ser His Phe Lys 
225             


<210>  8
<211>  228
<212>  PRT
<213>  人工序列（Artificial Sequence）

<220>
<223>  基质蛋白M（ΔL111，M51R）

<400>  8

Met Ser Ser Leu Lys Lys Ile Leu Gly Leu Lys Gly Lys Gly Lys Lys 
1               5                   10                  15      


Ser Lys Lys Leu Gly Ile Ala Pro Pro Pro Tyr Glu Glu Asp Thr Ser 
            20                  25                  30          


Met Glu Tyr Ala Pro Ser Ala Pro Ile Asp Lys Ser Tyr Phe Gly Val 
        35                  40                  45              


Asp Glu Arg Asp Thr Tyr Asp Pro Asn Gln Leu Arg Tyr Glu Lys Phe 
    50                  55                  60                  


Phe Phe Thr Val Lys Met Thr Val Arg Ser Asn Arg Pro Phe Arg Thr 
65                  70                  75                  80  


Tyr Ser Asp Val Ala Ala Ala Val Ser His Trp Asp His Met Tyr Ile 
                85                  90                  95      


Gly Met Ala Gly Lys Arg Pro Phe Tyr Lys Ile Leu Ala Phe Gly Ser 
            100                 105                 110         


Ser Asn Leu Lys Ala Thr Pro Ala Val Leu Ala Asp Gln Gly Gln Pro 
        115                 120                 125             


Glu Tyr His Ala His Cys Glu Gly Arg Ala Tyr Leu Pro His Arg Met 
    130                 135                 140                 


Gly Lys Thr Pro Pro Met Leu Asn Val Pro Glu His Phe Arg Arg Pro 
145                 150                 155                 160 


Phe Asn Ile Gly Leu Tyr Lys Gly Thr Ile Glu Leu Thr Met Thr Ile 
                165                 170                 175     


Tyr Asp Asp Glu Ser Leu Glu Ala Ala Pro Met Ile Trp Asp His Phe 
            180                 185                 190         


Asn Ser Ser Lys Phe Ser Asp Phe Arg Glu Lys Ala Leu Met Phe Gly 
        195                 200                 205             


Leu Ile Val Glu Lys Lys Ala Ser Gly Ala Trp Val Leu Asp Ser Ile 
    210                 215                 220                 


Ser His Phe Lys 
225             


<210>  9
<211>  228
<212>  PRT
<213>  人工序列（Artificial Sequence）

<220>
<223>  基质蛋白M（ΔL111，V221F）

<400>  9

Met Ser Ser Leu Lys Lys Ile Leu Gly Leu Lys Gly Lys Gly Lys Lys 
1               5                   10                  15      


Ser Lys Lys Leu Gly Ile Ala Pro Pro Pro Tyr Glu Glu Asp Thr Ser 
            20                  25                  30          


Met Glu Tyr Ala Pro Ser Ala Pro Ile Asp Lys Ser Tyr Phe Gly Val 
        35                  40                  45              


Asp Glu Met Asp Thr Tyr Asp Pro Asn Gln Leu Arg Tyr Glu Lys Phe 
    50                  55                  60                  


Phe Phe Thr Val Lys Met Thr Val Arg Ser Asn Arg Pro Phe Arg Thr 
65                  70                  75                  80  


Tyr Ser Asp Val Ala Ala Ala Val Ser His Trp Asp His Met Tyr Ile 
                85                  90                  95      


Gly Met Ala Gly Lys Arg Pro Phe Tyr Lys Ile Leu Ala Phe Gly Ser 
            100                 105                 110         


Ser Asn Leu Lys Ala Thr Pro Ala Val Leu Ala Asp Gln Gly Gln Pro 
        115                 120                 125             


Glu Tyr His Ala His Cys Glu Gly Arg Ala Tyr Leu Pro His Arg Met 
    130                 135                 140                 


Gly Lys Thr Pro Pro Met Leu Asn Val Pro Glu His Phe Arg Arg Pro 
145                 150                 155                 160 


Phe Asn Ile Gly Leu Tyr Lys Gly Thr Ile Glu Leu Thr Met Thr Ile 
                165                 170                 175     


Tyr Asp Asp Glu Ser Leu Glu Ala Ala Pro Met Ile Trp Asp His Phe 
            180                 185                 190         


Asn Ser Ser Lys Phe Ser Asp Phe Arg Glu Lys Ala Leu Met Phe Gly 
        195                 200                 205             


Leu Ile Val Glu Lys Lys Ala Ser Gly Ala Trp Phe Leu Asp Ser Ile 
    210                 215                 220                 


Ser His Phe Lys 
225             


<210>  10
<211>  228
<212>  PRT
<213>  人工序列（Artificial Sequence）

<220>
<223>  基质蛋白M（ΔL111，S226R）

<400>  10

Met Ser Ser Leu Lys Lys Ile Leu Gly Leu Lys Gly Lys Gly Lys Lys 
1               5                   10                  15      


Ser Lys Lys Leu Gly Ile Ala Pro Pro Pro Tyr Glu Glu Asp Thr Ser 
            20                  25                  30          


Met Glu Tyr Ala Pro Ser Ala Pro Ile Asp Lys Ser Tyr Phe Gly Val 
        35                  40                  45              


Asp Glu Met Asp Thr Tyr Asp Pro Asn Gln Leu Arg Tyr Glu Lys Phe 
    50                  55                  60                  


Phe Phe Thr Val Lys Met Thr Val Arg Ser Asn Arg Pro Phe Arg Thr 
65                  70                  75                  80  


Tyr Ser Asp Val Ala Ala Ala Val Ser His Trp Asp His Met Tyr Ile 
                85                  90                  95      


Gly Met Ala Gly Lys Arg Pro Phe Tyr Lys Ile Leu Ala Phe Gly Ser 
            100                 105                 110         


Ser Asn Leu Lys Ala Thr Pro Ala Val Leu Ala Asp Gln Gly Gln Pro 
        115                 120                 125             


Glu Tyr His Ala His Cys Glu Gly Arg Ala Tyr Leu Pro His Arg Met 
    130                 135                 140                 


Gly Lys Thr Pro Pro Met Leu Asn Val Pro Glu His Phe Arg Arg Pro 
145                 150                 155                 160 


Phe Asn Ile Gly Leu Tyr Lys Gly Thr Ile Glu Leu Thr Met Thr Ile 
                165                 170                 175     


Tyr Asp Asp Glu Ser Leu Glu Ala Ala Pro Met Ile Trp Asp His Phe 
            180                 185                 190         


Asn Ser Ser Lys Phe Ser Asp Phe Arg Glu Lys Ala Leu Met Phe Gly 
        195                 200                 205             


Leu Ile Val Glu Lys Lys Ala Ser Gly Ala Trp Val Leu Asp Ser Ile 
    210                 215                 220                 


Arg His Phe Lys 
225             


<210>  11
<211>  228
<212>  PRT
<213>  人工序列（Artificial Sequence）

<220>
<223>  基质蛋白M（ΔL111，V221F，S226R）

<400>  11

Met Ser Ser Leu Lys Lys Ile Leu Gly Leu Lys Gly Lys Gly Lys Lys 
1               5                   10                  15      


Ser Lys Lys Leu Gly Ile Ala Pro Pro Pro Tyr Glu Glu Asp Thr Ser 
            20                  25                  30          


Met Glu Tyr Ala Pro Ser Ala Pro Ile Asp Lys Ser Tyr Phe Gly Val 
        35                  40                  45              


Asp Glu Met Asp Thr Tyr Asp Pro Asn Gln Leu Arg Tyr Glu Lys Phe 
    50                  55                  60                  


Phe Phe Thr Val Lys Met Thr Val Arg Ser Asn Arg Pro Phe Arg Thr 
65                  70                  75                  80  


Tyr Ser Asp Val Ala Ala Ala Val Ser His Trp Asp His Met Tyr Ile 
                85                  90                  95      


Gly Met Ala Gly Lys Arg Pro Phe Tyr Lys Ile Leu Ala Phe Gly Ser 
            100                 105                 110         


Ser Asn Leu Lys Ala Thr Pro Ala Val Leu Ala Asp Gln Gly Gln Pro 
        115                 120                 125             


Glu Tyr His Ala His Cys Glu Gly Arg Ala Tyr Leu Pro His Arg Met 
    130                 135                 140                 


Gly Lys Thr Pro Pro Met Leu Asn Val Pro Glu His Phe Arg Arg Pro 
145                 150                 155                 160 


Phe Asn Ile Gly Leu Tyr Lys Gly Thr Ile Glu Leu Thr Met Thr Ile 
                165                 170                 175     


Tyr Asp Asp Glu Ser Leu Glu Ala Ala Pro Met Ile Trp Asp His Phe 
            180                 185                 190         


Asn Ser Ser Lys Phe Ser Asp Phe Arg Glu Lys Ala Leu Met Phe Gly 
        195                 200                 205             


Leu Ile Val Glu Lys Lys Ala Ser Gly Ala Trp Phe Leu Asp Ser Ile 
    210                 215                 220                 


Arg His Phe Lys 
225             

<210>  12
<211>  120
<212>  PRT
<213>  人工序列（Artificial Sequence）

<220>
<223>  PD-1抗体VH

<400>  12

Gln Val Gln Leu Val Gln Ser Gly Val Glu Val Lys Lys Pro Gly Ala 
1               5                   10                  15      


Ser Val Lys Val Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Asn Tyr 
            20                  25                  30          


Tyr Met Tyr Trp Val Arg Gln Ala Pro Gly Gln Gly Leu Glu Trp Met 
        35                  40                  45              


Gly Gly Ile Asn Pro Ser Asn Gly Gly Thr Asn Phe Asn Glu Lys Phe 
    50                  55                  60                  


Lys Asn Arg Val Thr Leu Thr Thr Asp Ser Ser Thr Thr Thr Ala Tyr 
65                  70                  75                  80  


Met Glu Leu Lys Ser Leu Gln Phe Asp Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Arg Asp Tyr Arg Phe Asp Met Gly Phe Asp Tyr Trp Gly Gln 
            100                 105                 110         


Gly Thr Thr Val Thr Val Ser Ser 
        115                 120 


<210>  13
<211>  112
<212>  PRT
<213>  人工序列（Artificial Sequence）

<220>
<223>  PD-1抗体VL

<400>  13

Glu Ile Val Leu Thr Gln Ser Pro Ala Thr Leu Ser Leu Ser Pro Gly 
1               5                   10                  15      


Glu Arg Ala Thr Leu Ser Cys Arg Ala Ser Lys Gly Val Ser Thr Ser 
            20                  25                  30          


Gly Tyr Ser Tyr Leu His Trp Tyr Gln Gln Lys Pro Gly Gln Ala Pro 
        35                  40                  45              


Arg Leu Leu Ile Tyr Leu Ala Ser Tyr Leu Glu Ser Gly Val Pro Ala 
    50                  55                  60                  


Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser 
65                  70                  75                  80  


Ser Leu Glu Pro Glu Asp Phe Ala Val Tyr Tyr Cys Gln His Ser Arg 
                85                  90                  95      


Asp Leu Pro Leu Thr Phe Gly Gly Gly Thr Lys Val Glu Ile Lys Arg 
            100                 105                 110         
         