                         SEQUENCE LISTING

<110>  ҩҵϺɷ޹˾
 
<120>  һֿPD-1PD-L1ļ˫Կ

<130>  SH363-21P450348

<160>  32    

<170>  PatentIn version 3.5

<210>  1
<211>  5
<212>  PRT
<213>  Mus musculus

<400>  1

Ser Tyr Gly Val His 
1               5   


<210>  2
<211>  16
<212>  PRT
<213>  Mus musculus

<400>  2

Leu Ile Trp Ser Gly Gly Gly Thr Asp Tyr Asn Ala Ala Phe Ile Ser 
1               5                   10                  15      


<210>  3
<211>  9
<212>  PRT
<213>  Mus musculus

<400>  3

Gln Leu Gly Leu Arg Ala Met Asp Tyr 
1               5                   


<210>  4
<211>  11
<212>  PRT
<213>  Mus musculus

<400>  4

Arg Ala Ser Gln Ser Ile Gly Thr Thr Ile His 
1               5                   10      


<210>  5
<211>  7
<212>  PRT
<213>  Mus musculus

<400>  5

Tyr Ala Ser Glu Ser Val Ser 
1               5           


<210>  6
<211>  9
<212>  PRT
<213>  Mus musculus

<400>  6

Gln Gln Ser Asn Ser Trp Pro Leu Thr 
1               5                   


<210>  7
<211>  11
<212>  PRT
<213>  Artificial

<220>
<223>  ĸ

<400>  7

Trp Gly Gln Gly Thr Ser Val Thr Val Ser Ser 
1               5                   10      


<210>  8
<211>  10
<212>  PRT
<213>  Artificial

<220>
<223>  ĸ

<400>  8

Phe Gly Ala Gly Thr Lys Leu Glu Ile Lys 
1               5                   10  


<210>  9
<211>  117
<212>  PRT
<213>  Artificial

<220>
<223>  Anti-PDL1ɱİ

<400>  9

Gln Val Gln Leu Gln Gln Ser Gly Gly Gly Leu Val Lys Pro Ser Gln 
1               5                   10                  15      


Ser Leu Ser Leu Thr Cys Thr Val Ser Gly Phe Ser Leu Thr Ser Tyr 
            20                  25                  30          


Gly Val His Trp Val Arg Gln Pro Pro Gly Lys Gly Leu Glu Trp Ile 
        35                  40                  45              


Gly Leu Ile Trp Ser Gly Gly Gly Thr Asp Tyr Asn Pro Ser Leu Lys 
    50                  55                  60                  


Ser Arg Leu Thr Ile Ser Arg Asp Thr Ser Lys Asn Gln Val Ser Phe 
65                  70                  75                  80  


Lys Ile Ser Ser Leu Thr Ala Ala Asp Thr Ala Val Tyr Tyr Cys Ala 
                85                  90                  95      


Arg Gln Leu Gly Leu Arg Ala Met Asp Tyr Trp Gly Gln Gly Thr Ser 
            100                 105                 110         


Val Thr Val Ser Ser 
        115         


<210>  10
<211>  107
<212>  PRT
<213>  Artificial

<220>
<223>  Anti-PDL1ɱİ

<400>  10

Glu Ile Val Leu Thr Gln Ser Pro Asp Phe Leu Ser Val Thr Pro Lys 
1               5                   10                  15      


Glu Lys Val Thr Ile Thr Cys Arg Ala Ser Gln Ser Ile Gly Thr Thr 
            20                  25                  30          


Ile His Trp Tyr Gln Gln Lys Pro Asp Gln Ser Pro Lys Leu Leu Ile 
        35                  40                  45              


Lys Tyr Ala Ser Gln Ser Phe Ser Gly Val Pro Ser Arg Phe Ser Gly 
    50                  55                  60                  


Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Asn Ser Val Glu Ala 
65                  70                  75                  80  


Glu Asp Ala Ala Thr Tyr Tyr Cys Gln Gln Ser Asn Ser Trp Pro Leu 
                85                  90                  95      


Thr Phe Gly Ala Gly Thr Lys Leu Glu Ile Lys 
            100                 105         


<210>  11
<211>  330
<212>  PRT
<213>  Artificial

<220>
<223>  IgG1㶨İ

<400>  11

Ala Ser Thr Lys Gly Pro Ser Val Phe Pro Leu Ala Pro Ser Ser Lys 
1               5                   10                  15      


Ser Thr Ser Gly Gly Thr Ala Ala Leu Gly Cys Leu Val Lys Asp Tyr 
            20                  25                  30          


Phe Pro Glu Pro Val Thr Val Ser Trp Asn Ser Gly Ala Leu Thr Ser 
        35                  40                  45              


Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Ser Gly Leu Tyr Ser 
    50                  55                  60                  


Leu Ser Ser Val Val Thr Val Pro Ser Ser Ser Leu Gly Thr Gln Thr 
65                  70                  75                  80  


Tyr Ile Cys Asn Val Asn His Lys Pro Ser Asn Thr Lys Val Asp Lys 
                85                  90                  95      


Lys Val Glu Pro Lys Ser Cys Asp Lys Thr His Thr Cys Pro Pro Cys 
            100                 105                 110         


Pro Ala Pro Glu Leu Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro 
        115                 120                 125             


Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys 
    130                 135                 140                 


Val Val Val Asp Val Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp 
145                 150                 155                 160 


Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu 
                165                 170                 175     


Glu Gln Tyr Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu 
            180                 185                 190         


His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn 
        195                 200                 205             


Lys Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly 
    210                 215                 220                 


Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Glu Glu 
225                 230                 235                 240 


Met Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr 
                245                 250                 255     


Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn 
            260                 265                 270         


Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe 
        275                 280                 285             


Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn 
    290                 295                 300                 


Val Phe Ser Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr 
305                 310                 315                 320 


Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys 
                325                 330 


<210>  12
<211>  107
<212>  PRT
<213>  Artificial

<220>
<223>  Kappa㶨İ

<400>  12

Arg Thr Val Ala Ala Pro Ser Val Phe Ile Phe Pro Pro Ser Asp Glu 
1               5                   10                  15      


Gln Leu Lys Ser Gly Thr Ala Ser Val Val Cys Leu Leu Asn Asn Phe 
            20                  25                  30          


Tyr Pro Arg Glu Ala Lys Val Gln Trp Lys Val Asp Asn Ala Leu Gln 
        35                  40                  45              


Ser Gly Asn Ser Gln Glu Ser Val Thr Glu Gln Asp Ser Lys Asp Ser 
    50                  55                  60                  


Thr Tyr Ser Leu Ser Ser Thr Leu Thr Leu Ser Lys Ala Asp Tyr Glu 
65                  70                  75                  80  


Lys His Lys Val Tyr Ala Cys Glu Val Thr His Gln Gly Leu Ser Ser 
                85                  90                  95      


Pro Val Thr Lys Ser Phe Asn Arg Gly Glu Cys 
            100                 105         


<210>  13
<211>  447
<212>  PRT
<213>  Artificial

<220>
<223>  Anti-PDL1İ

<400>  13

Gln Val Gln Leu Gln Gln Ser Gly Gly Gly Leu Val Lys Pro Ser Gln 
1               5                   10                  15      


Ser Leu Ser Leu Thr Cys Thr Val Ser Gly Phe Ser Leu Thr Ser Tyr 
            20                  25                  30          


Gly Val His Trp Val Arg Gln Pro Pro Gly Lys Gly Leu Glu Trp Ile 
        35                  40                  45              


Gly Leu Ile Trp Ser Gly Gly Gly Thr Asp Tyr Asn Pro Ser Leu Lys 
    50                  55                  60                  


Ser Arg Leu Thr Ile Ser Arg Asp Thr Ser Lys Asn Gln Val Ser Phe 
65                  70                  75                  80  


Lys Ile Ser Ser Leu Thr Ala Ala Asp Thr Ala Val Tyr Tyr Cys Ala 
                85                  90                  95      


Arg Gln Leu Gly Leu Arg Ala Met Asp Tyr Trp Gly Gln Gly Thr Ser 
            100                 105                 110         


Val Thr Val Ser Ser Ala Ser Thr Lys Gly Pro Ser Val Phe Pro Leu 
        115                 120                 125             


Ala Pro Ser Ser Lys Ser Thr Ser Gly Gly Thr Ala Ala Leu Gly Cys 
    130                 135                 140                 


Leu Val Lys Asp Tyr Phe Pro Glu Pro Val Thr Val Ser Trp Asn Ser 
145                 150                 155                 160 


Gly Ala Leu Thr Ser Gly Val His Thr Phe Pro Ala Val Leu Gln Ser 
                165                 170                 175     


Ser Gly Leu Tyr Ser Leu Ser Ser Val Val Thr Val Pro Ser Ser Ser 
            180                 185                 190         


Leu Gly Thr Gln Thr Tyr Ile Cys Asn Val Asn His Lys Pro Ser Asn 
        195                 200                 205             


Thr Lys Val Asp Lys Lys Val Glu Pro Lys Ser Cys Asp Lys Thr His 
    210                 215                 220                 


Thr Cys Pro Pro Cys Pro Ala Pro Glu Leu Leu Gly Gly Pro Ser Val 
225                 230                 235                 240 


Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr 
                245                 250                 255     


Pro Glu Val Thr Cys Val Val Val Asp Val Ser His Glu Asp Pro Glu 
            260                 265                 270         


Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala Lys 
        275                 280                 285             


Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr Arg Val Val Ser 
    290                 295                 300                 


Val Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys 
305                 310                 315                 320 


Cys Lys Val Ser Asn Lys Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile 
                325                 330                 335     


Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro 
            340                 345                 350         


Pro Ser Arg Glu Glu Met Thr Lys Asn Gln Val Ser Leu Thr Cys Leu 
        355                 360                 365             


Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn 
    370                 375                 380                 


Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser 
385                 390                 395                 400 


Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg 
                405                 410                 415     


Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala Leu 
            420                 425                 430         


His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys 
        435                 440                 445         


<210>  14
<211>  1341
<212>  DNA
<213>  Artificial

<220>
<223>  Anti-PDL1ĺ

<400>  14
caggtccagc tgcagcagtc aggagggggc ctggtgaagc catcacagag cctgtccctg       60

acctgcacag tctctgggtt cagtctgact tcatacggag tgcactgggt ccgacagccc      120

cctggaaagg gactggagtg gatcggcctg atttggtctg gcgggggaac agactataac      180

cccagcctga aatcccggct gaccatctct agagatacca gtaagaatca agtgagcttt      240

aaaattagct ccctgacagc cgctgacact gcagtgtact attgtgcaag gcagctggga      300

ctgcgagcta tggattactg gggacagggc acttccgtga ccgtctctag tgcgagcacc      360

aagggacctt ccgtgtttcc cctcgccccc agctccaaaa gcaccagcgg cggaacagct      420

gctctcggct gtctcgtcaa ggattacttc cccgagcccg tgaccgtgag ctggaacagc      480

ggagccctga caagcggcgt ccacaccttc cctgctgtcc tacagtcctc cggactgtac      540

agcctgagca gcgtggtgac agtccctagc agctccctgg gcacccagac atatatttgc      600

aacgtgaatc acaagcccag caacaccaag gtcgataaga aggtggagcc taagtcctgc      660

gacaagaccc acacatgtcc cccctgtccc gctcctgaac tgctgggagg cccttccgtg      720

ttcctgttcc cccctaagcc caaggacacc ctgatgattt ccaggacacc cgaggtgacc      780

tgtgtggtgg tggacgtcag ccacgaggac cccgaggtga aattcaactg gtacgtcgat      840

ggcgtggagg tgcacaacgc taagaccaag cccagggagg agcagtacaa ttccacctac      900

agggtggtgt ccgtgctgac cgtcctccat caggactggc tgaacggcaa agagtataag      960

tgcaaggtga gcaacaaggc cctccctgct cccatcgaga agaccatcag caaagccaag     1020

ggccagccca gggaacctca agtctatacc ctgcctccca gcagggagga gatgaccaag     1080

aaccaagtga gcctcacatg cctcgtcaag ggcttctatc cttccgatat tgccgtcgag     1140

tgggagtcca acggacagcc cgagaacaac tacaagacaa caccccccgt gctcgattcc     1200

gatggcagct tcttcctgta ctccaagctg accgtggaca agtccagatg gcaacaaggc     1260

aacgtcttca gttgcagcgt catgcatgag gccctccaca accactacac ccagaagagc     1320

ctctccctga gccctggaaa g                                               1341


<210>  15
<211>  214
<212>  PRT
<213>  Artificial

<220>
<223>  Anti-PDL1İ

<400>  15

Glu Ile Val Leu Thr Gln Ser Pro Asp Phe Leu Ser Val Thr Pro Lys 
1               5                   10                  15      


Glu Lys Val Thr Ile Thr Cys Arg Ala Ser Gln Ser Ile Gly Thr Thr 
            20                  25                  30          


Ile His Trp Tyr Gln Gln Lys Pro Asp Gln Ser Pro Lys Leu Leu Ile 
        35                  40                  45              


Lys Tyr Ala Ser Gln Ser Phe Ser Gly Val Pro Ser Arg Phe Ser Gly 
    50                  55                  60                  


Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Asn Ser Val Glu Ala 
65                  70                  75                  80  


Glu Asp Ala Ala Thr Tyr Tyr Cys Gln Gln Ser Asn Ser Trp Pro Leu 
                85                  90                  95      


Thr Phe Gly Ala Gly Thr Lys Leu Glu Ile Lys Arg Thr Val Ala Ala 
            100                 105                 110         


Pro Ser Val Phe Ile Phe Pro Pro Ser Asp Glu Gln Leu Lys Ser Gly 
        115                 120                 125             


Thr Ala Ser Val Val Cys Leu Leu Asn Asn Phe Tyr Pro Arg Glu Ala 
    130                 135                 140                 


Lys Val Gln Trp Lys Val Asp Asn Ala Leu Gln Ser Gly Asn Ser Gln 
145                 150                 155                 160 


Glu Ser Val Thr Glu Gln Asp Ser Lys Asp Ser Thr Tyr Ser Leu Ser 
                165                 170                 175     


Ser Thr Leu Thr Leu Ser Lys Ala Asp Tyr Glu Lys His Lys Val Tyr 
            180                 185                 190         


Ala Cys Glu Val Thr His Gln Gly Leu Ser Ser Pro Val Thr Lys Ser 
        195                 200                 205             


Phe Asn Arg Gly Glu Cys 
    210                 


<210>  16
<211>  642
<212>  DNA
<213>  Artificial

<220>
<223>  Anti-PDL1ĺ

<400>  16
gaaatcgtgc tgacacagag ccctgacttt ctgtccgtga cacccaagga gaaagtcact       60

atcacctgcc gggctagcca gtccatcgga accacaattc actggtacca gcagaagccc      120

gaccagagcc ctaagctgct gattaaatat gcctctcaga gtttctcagg cgtgccatcc      180

agatttagcg gctccgggtc tggaactgac ttcacactga ctatcaactc tgtcgaggca      240

gaagatgccg ctacctacta ttgtcagcag agtaattcat ggcccctgac ctttggcgcc      300

gggacaaagc tggaaattaa aagaaccgtc gccgctccca gcgtcttcat cttccccccc      360

agcgatgagc agctgaagag cggaaccgcc agcgtggtgt gcctgctgaa caacttctac      420

cccagggagg ccaaggtgca atggaaggtg gacaacgccc tacagagcgg caactcccag      480

gagagcgtga ccgagcagga cagcaaggat agcacctaca gcctgagcag caccctcacc      540

ctgagcaagg ccgactacga gaagcacaag gtgtacgcct gcgaggtgac ccatcagggc      600

ctgagcagcc ctgtgaccaa gagcttcaac aggggcgagt gc                         642


<210>  17
<211>  5
<212>  PRT
<213>  Artificial

<220>
<223>  Anti-PDL1H-CDR1İ

<400>  17

Ser Tyr Gly Val His 
1               5   


<210>  18
<211>  16
<212>  PRT
<213>  Artificial

<220>
<223>  Anti-PDL1H-CDR2İ

<400>  18

Leu Ile Trp Ser Gly Gly Gly Thr Asp Tyr Asn Pro Ser Leu Lys Ser 
1               5                   10                  15      


<210>  19
<211>  9
<212>  PRT
<213>  Artificial

<220>
<223>  Anti-PDL1H-CDR3İ

<400>  19

Gln Leu Gly Leu Arg Ala Met Asp Tyr 
1               5                   


<210>  20
<211>  11
<212>  PRT
<213>  Artificial

<220>
<223>  Anti-PDL1L-CDR1İ

<400>  20

Arg Ala Ser Gln Ser Ile Gly Thr Thr Ile His 
1               5                   10      


<210>  21
<211>  7
<212>  PRT
<213>  Artificial

<220>
<223>  Anti-PDL1L-CDR2İ

<400>  21

Tyr Ala Ser Gln Ser Phe Ser 
1               5           


<210>  22
<211>  9
<212>  PRT
<213>  Artificial

<220>
<223>  Anti-PDL1L-CDR3İ

<400>  22

Gln Gln Ser Asn Ser Trp Pro Leu Thr 
1               5                   


<210>  23
<211>  118
<212>  PRT
<213>  Artificial

<220>
<223>  Atezolizumabɱİ

<400>  23

Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Asp Ser 
            20                  25                  30          


Trp Ile His Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val 
        35                  40                  45              


Ala Trp Ile Ser Pro Tyr Gly Gly Ser Thr Tyr Tyr Ala Asp Ser Val 
    50                  55                  60                  


Lys Gly Arg Phe Thr Ile Ser Ala Asp Thr Ser Lys Asn Thr Ala Tyr 
65                  70                  75                  80  


Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Arg His Trp Pro Gly Gly Phe Asp Tyr Trp Gly Gln Gly Thr 
            100                 105                 110         


Leu Val Thr Val Ser Ser 
        115             


<210>  24
<211>  107
<212>  PRT
<213>  Artificial

<220>
<223>  Atezolizumabɱİ

<400>  24

Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly 
1               5                   10                  15      


Asp Arg Val Thr Ile Thr Cys Arg Ala Ser Gln Asp Val Ser Thr Ala 
            20                  25                  30          


Val Ala Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Leu Leu Ile 
        35                  40                  45              


Tyr Ser Ala Ser Phe Leu Tyr Ser Gly Val Pro Ser Arg Phe Ser Gly 
    50                  55                  60                  


Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu Gln Pro 
65                  70                  75                  80  


Glu Asp Phe Ala Thr Tyr Tyr Cys Gln Gln Tyr Leu Tyr His Pro Ala 
                85                  90                  95      


Thr Phe Gly Gln Gly Thr Lys Val Glu Ile Lys 
            100                 105         


<210>  25
<211>  117
<212>  PRT
<213>  Artificial

<220>
<223>  mAb1-25-Hu609ɱİ

<400>  25

Glu Val Lys Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Ala Phe Ser Ser Tyr 
            20                  25                  30          


Asp Met Ser Trp Val Arg Gln Ala Pro Gly Lys Arg Leu Glu Trp Val 
        35                  40                  45              


Ala Thr Ile Ser Gly Gly Gly Arg Tyr Thr Tyr Tyr Pro Asp Thr Val 
    50                  55                  60                  


Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys Asn Ser His Tyr 
65                  70                  75                  80  


Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Phe Cys 
                85                  90                  95      


Ala Ser Pro Tyr Gly Gly Tyr Phe Asp Val Trp Gly Gln Gly Thr Leu 
            100                 105                 110         


Val Thr Val Ser Ser 
        115         


<210>  26
<211>  107
<212>  PRT
<213>  Artificial

<220>
<223>  mAb1-25-Hu609ɱİ

<400>  26

Glu Ile Val Leu Thr Gln Ser Pro Ala Thr Leu Ser Leu Ser Pro Gly 
1               5                   10                  15      


Glu Arg Ala Thr Leu Ser Cys Arg Ala Ser Gln Ser Ile Ser Asn Phe 
            20                  25                  30          


Leu His Trp Tyr Gln Gln Lys Pro Gly Gln Ala Pro Arg Leu Leu Ile 
        35                  40                  45              


Lys Tyr Ala Ser Gln Ser Ile Ser Gly Ile Pro Ala Arg Phe Ser Gly 
    50                  55                  60                  


Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu Glu Pro 
65                  70                  75                  80  


Glu Asp Phe Ala Val Tyr Phe Cys Gln Gln Ser Asn Ser Trp Pro His 
                85                  90                  95      


Thr Phe Gly Gln Gly Thr Lys Val Glu Ile Lys 
            100                 105         


<210>  27
<211>  327
<212>  PRT
<213>  Artificial

<220>
<223>  IgG4S228P㶨İ

<400>  27

Ala Ser Thr Lys Gly Pro Ser Val Phe Pro Leu Ala Pro Cys Ser Arg 
1               5                   10                  15      


Ser Thr Ser Glu Ser Thr Ala Ala Leu Gly Cys Leu Val Lys Asp Tyr 
            20                  25                  30          


Phe Pro Glu Pro Val Thr Val Ser Trp Asn Ser Gly Ala Leu Thr Ser 
        35                  40                  45              


Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Ser Gly Leu Tyr Ser 
    50                  55                  60                  


Leu Ser Ser Val Val Thr Val Pro Ser Ser Ser Leu Gly Thr Lys Thr 
65                  70                  75                  80  


Tyr Thr Cys Asn Val Asp His Lys Pro Ser Asn Thr Lys Val Asp Lys 
                85                  90                  95      


Arg Val Glu Ser Lys Tyr Gly Pro Pro Cys Pro Pro Cys Pro Ala Pro 
            100                 105                 110         


Glu Phe Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys 
        115                 120                 125             


Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val 
    130                 135                 140                 


Asp Val Ser Gln Glu Asp Pro Glu Val Gln Phe Asn Trp Tyr Val Asp 
145                 150                 155                 160 


Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Phe 
                165                 170                 175     


Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp 
            180                 185                 190         


Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Gly Leu 
        195                 200                 205             


Pro Ser Ser Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg 
    210                 215                 220                 


Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Gln Glu Glu Met Thr Lys 
225                 230                 235                 240 


Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp 
                245                 250                 255     


Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys 
            260                 265                 270         


Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser 
        275                 280                 285             


Arg Leu Thr Val Asp Lys Ser Arg Trp Gln Glu Gly Asn Val Phe Ser 
    290                 295                 300                 


Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser 
305                 310                 315                 320 


Leu Ser Leu Ser Leu Gly Lys 
                325         


<210>  28
<211>  15
<212>  PRT
<213>  Artificial

<220>
<223>  ӣGGGGSGGGGSGGGGS

<400>  28

Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
1               5                   10                  15  


<210>  29
<211>  674
<212>  PRT
<213>  Artificial

<220>
<223>  PDL1-Fab-609-IgG4İ

<400>  29

Gln Val Gln Leu Gln Gln Ser Gly Gly Gly Leu Val Lys Pro Ser Gln 
1               5                   10                  15      


Ser Leu Ser Leu Thr Cys Thr Val Ser Gly Phe Ser Leu Thr Ser Tyr 
            20                  25                  30          


Gly Val His Trp Val Arg Gln Pro Pro Gly Lys Gly Leu Glu Trp Ile 
        35                  40                  45              


Gly Leu Ile Trp Ser Gly Gly Gly Thr Asp Tyr Asn Pro Ser Leu Lys 
    50                  55                  60                  


Ser Arg Leu Thr Ile Ser Arg Asp Thr Ser Lys Asn Gln Val Ser Phe 
65                  70                  75                  80  


Lys Ile Ser Ser Leu Thr Ala Ala Asp Thr Ala Val Tyr Tyr Cys Ala 
                85                  90                  95      


Arg Gln Leu Gly Leu Arg Ala Met Asp Tyr Trp Gly Gln Gly Thr Ser 
            100                 105                 110         


Val Thr Val Ser Ser Ala Ser Thr Lys Gly Pro Ser Val Phe Pro Leu 
        115                 120                 125             


Ala Pro Cys Ser Arg Ser Thr Ser Glu Ser Thr Ala Ala Leu Gly Cys 
    130                 135                 140                 


Leu Val Lys Asp Tyr Phe Pro Glu Pro Val Thr Val Ser Trp Asn Ser 
145                 150                 155                 160 


Gly Ala Leu Thr Ser Gly Val His Thr Phe Pro Ala Val Leu Gln Ser 
                165                 170                 175     


Ser Gly Leu Tyr Ser Leu Ser Ser Val Val Thr Val Pro Ser Ser Ser 
            180                 185                 190         


Leu Gly Thr Lys Thr Tyr Thr Cys Asn Val Asp His Lys Pro Ser Asn 
        195                 200                 205             


Thr Lys Val Asp Lys Arg Val Gly Gly Gly Gly Ser Gly Gly Gly Gly 
    210                 215                 220                 


Ser Gly Gly Gly Gly Ser Glu Val Lys Leu Val Glu Ser Gly Gly Gly 
225                 230                 235                 240 


Leu Val Gln Pro Gly Gly Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly 
                245                 250                 255     


Phe Ala Phe Ser Ser Tyr Asp Met Ser Trp Val Arg Gln Ala Pro Gly 
            260                 265                 270         


Lys Arg Leu Glu Trp Val Ala Thr Ile Ser Gly Gly Gly Arg Tyr Thr 
        275                 280                 285             


Tyr Tyr Pro Asp Thr Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn 
    290                 295                 300                 


Ala Lys Asn Ser His Tyr Leu Gln Met Asn Ser Leu Arg Ala Glu Asp 
305                 310                 315                 320 


Thr Ala Val Tyr Phe Cys Ala Ser Pro Tyr Gly Gly Tyr Phe Asp Val 
                325                 330                 335     


Trp Gly Gln Gly Thr Leu Val Thr Val Ser Ser Ala Ser Thr Lys Gly 
            340                 345                 350         


Pro Ser Val Phe Pro Leu Ala Pro Cys Ser Arg Ser Thr Ser Glu Ser 
        355                 360                 365             


Thr Ala Ala Leu Gly Cys Leu Val Lys Asp Tyr Phe Pro Glu Pro Val 
    370                 375                 380                 


Thr Val Ser Trp Asn Ser Gly Ala Leu Thr Ser Gly Val His Thr Phe 
385                 390                 395                 400 


Pro Ala Val Leu Gln Ser Ser Gly Leu Tyr Ser Leu Ser Ser Val Val 
                405                 410                 415     


Thr Val Pro Ser Ser Ser Leu Gly Thr Lys Thr Tyr Thr Cys Asn Val 
            420                 425                 430         


Asp His Lys Pro Ser Asn Thr Lys Val Asp Lys Arg Val Glu Ser Lys 
        435                 440                 445             


Tyr Gly Pro Pro Cys Pro Pro Cys Pro Ala Pro Glu Phe Leu Gly Gly 
    450                 455                 460                 


Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile 
465                 470                 475                 480 


Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser Gln Glu 
                485                 490                 495     


Asp Pro Glu Val Gln Phe Asn Trp Tyr Val Asp Gly Val Glu Val His 
            500                 505                 510         


Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Phe Asn Ser Thr Tyr Arg 
        515                 520                 525             


Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys 
    530                 535                 540                 


Glu Tyr Lys Cys Lys Val Ser Asn Lys Gly Leu Pro Ser Ser Ile Glu 
545                 550                 555                 560 


Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr 
                565                 570                 575     


Thr Leu Pro Pro Ser Gln Glu Glu Met Thr Lys Asn Gln Val Ser Leu 
            580                 585                 590         


Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp 
        595                 600                 605             


Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val 
    610                 615                 620                 


Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Arg Leu Thr Val Asp 
625                 630                 635                 640 


Lys Ser Arg Trp Gln Glu Gly Asn Val Phe Ser Cys Ser Val Met His 
                645                 650                 655     


Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Leu 
            660                 665                 670         


Gly Lys 
        


<210>  30
<211>  2022
<212>  DNA
<213>  Artificial

<220>
<223>  PDL1-Fab-609-IgG4ĺ

<400>  30
caggtccagc tgcagcagtc aggagggggc ctggtgaagc catcacagag cctgtccctg       60

acctgcacag tctctgggtt cagtctgact tcatacggag tgcactgggt ccgacagccc      120

cctggaaagg gactggagtg gatcggcctg atttggtctg gcgggggaac agactataac      180

cccagcctga aatcccggct gaccatctct agagatacca gtaagaatca agtgagcttt      240

aaaattagct ccctgacagc cgctgacact gcagtgtact attgtgcaag gcagctggga      300

ctgcgagcta tggattactg gggacagggc acttccgtga ccgtctctag tgcaagtacc      360

aagggaccta gtgttttccc tcttgcacct tgctccaggt caacatcaga gtccacagct      420

gctcttggat gtctcgttaa ggactacttc ccagagccag ttaccgtatc ctggaactcc      480

ggagctttga caagcggcgt tcatacattc ccagctgtgt tgcagagttc tgggttgtac      540

agtttgagct cagtggtgac cgtgccttca tcttctttgg gcactaagac ctacacctgc      600

aacgtggatc acaagccaag caacaccaag gtggataaga gggtgggtgg aggcggttca      660

ggcggaggtg gcagcggagg tggcgggagt gaggtcaagc tggtggaaag cggcggcggc      720

ctggtgcagc ctggaggatc cctgcggctg agctgcgctg cctccggctt cgctttcagc      780

tcctatgaca tgtcctgggt gaggcaggcc cctggaaaga ggctggagtg ggtggctacc      840

atctccggag gcggaaggta cacctactac cccgacacag tgaagggaag gttcaccatc      900

agccgggata acgccaaaaa cagccactat ctccagatga actccctgag ggccgaagat      960

acagccgtgt atttctgtgc ctccccctac ggaggctatt ttgacgtgtg gggacagggc     1020

accctggtga ccgtctcctc cgcaagtacc aagggaccta gtgttttccc tcttgcacct     1080

tgctccaggt caacatcaga gtccacagct gctcttggat gtctcgttaa ggactacttc     1140

ccagagccag ttaccgtatc ctggaactcc ggagctttga caagcggcgt tcatacattc     1200

ccagctgtgt tgcagagttc tgggttgtac agtttgagct cagtggtgac cgtgccttca     1260

tcttctttgg gcactaagac ctacacctgc aacgtggatc acaagccaag caacaccaag     1320

gtggataaga gggtggagtc caagtacggc ccaccatgtc ctccatgtcc agcccctgaa     1380

tttttgggcg ggccttctgt ctttctgttt cctcctaaac ctaaagatac cctgatgatc     1440

agccgcacac ccgaagtcac ttgtgtggtc gtggatgtgt ctcaggaaga tcccgaagtg     1500

cagtttaact ggtatgtcga tggcgtggaa gtgcataatg ccaaaactaa gccccgcgaa     1560

gaacagttca acagcactta tcgggtcgtg tctgtgctca cagtcctcca tcaggattgg     1620

ctgaatggga aagaatataa gtgcaaggtg agcaataagg gcctccccag cagcatcgag     1680

aagactatta gcaaagccaa agggcagcca cgggaacccc aggtgtacac tctgcccccc     1740

tctcaggagg agatgactaa aaatcaggtc tctctgactt gtctggtgaa agggttttat     1800

cccagcgaca ttgccgtgga gtgggagtct aatggccagc ccgagaataa ttataagaca     1860

actccccccg tcctggactc tgacggcagc tttttcctgt attctcggct gacagtggac     1920

aaaagtcgct ggcaggaggg caatgtcttt agttgcagtg tcatgcatga ggccctgcac     1980

aatcactata cacagaaaag cctgtctctg agtctgggca aa                        2022


<210>  31
<211>  674
<212>  PRT
<213>  Artificial

<220>
<223>  609-Fab-PDL1-IgG4İ

<400>  31

Glu Val Lys Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Ala Phe Ser Ser Tyr 
            20                  25                  30          


Asp Met Ser Trp Val Arg Gln Ala Pro Gly Lys Arg Leu Glu Trp Val 
        35                  40                  45              


Ala Thr Ile Ser Gly Gly Gly Arg Tyr Thr Tyr Tyr Pro Asp Thr Val 
    50                  55                  60                  


Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys Asn Ser His Tyr 
65                  70                  75                  80  


Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Phe Cys 
                85                  90                  95      


Ala Ser Pro Tyr Gly Gly Tyr Phe Asp Val Trp Gly Gln Gly Thr Leu 
            100                 105                 110         


Val Thr Val Ser Ser Ala Ser Thr Lys Gly Pro Ser Val Phe Pro Leu 
        115                 120                 125             


Ala Pro Cys Ser Arg Ser Thr Ser Glu Ser Thr Ala Ala Leu Gly Cys 
    130                 135                 140                 


Leu Val Lys Asp Tyr Phe Pro Glu Pro Val Thr Val Ser Trp Asn Ser 
145                 150                 155                 160 


Gly Ala Leu Thr Ser Gly Val His Thr Phe Pro Ala Val Leu Gln Ser 
                165                 170                 175     


Ser Gly Leu Tyr Ser Leu Ser Ser Val Val Thr Val Pro Ser Ser Ser 
            180                 185                 190         


Leu Gly Thr Lys Thr Tyr Thr Cys Asn Val Asp His Lys Pro Ser Asn 
        195                 200                 205             


Thr Lys Val Asp Lys Arg Val Gly Gly Gly Gly Ser Gly Gly Gly Gly 
    210                 215                 220                 


Ser Gly Gly Gly Gly Ser Gln Val Gln Leu Gln Gln Ser Gly Gly Gly 
225                 230                 235                 240 


Leu Val Lys Pro Ser Gln Ser Leu Ser Leu Thr Cys Thr Val Ser Gly 
                245                 250                 255     


Phe Ser Leu Thr Ser Tyr Gly Val His Trp Val Arg Gln Pro Pro Gly 
            260                 265                 270         


Lys Gly Leu Glu Trp Ile Gly Leu Ile Trp Ser Gly Gly Gly Thr Asp 
        275                 280                 285             


Tyr Asn Pro Ser Leu Lys Ser Arg Leu Thr Ile Ser Arg Asp Thr Ser 
    290                 295                 300                 


Lys Asn Gln Val Ser Phe Lys Ile Ser Ser Leu Thr Ala Ala Asp Thr 
305                 310                 315                 320 


Ala Val Tyr Tyr Cys Ala Arg Gln Leu Gly Leu Arg Ala Met Asp Tyr 
                325                 330                 335     


Trp Gly Gln Gly Thr Ser Val Thr Val Ser Ser Ala Ser Thr Lys Gly 
            340                 345                 350         


Pro Ser Val Phe Pro Leu Ala Pro Cys Ser Arg Ser Thr Ser Glu Ser 
        355                 360                 365             


Thr Ala Ala Leu Gly Cys Leu Val Lys Asp Tyr Phe Pro Glu Pro Val 
    370                 375                 380                 


Thr Val Ser Trp Asn Ser Gly Ala Leu Thr Ser Gly Val His Thr Phe 
385                 390                 395                 400 


Pro Ala Val Leu Gln Ser Ser Gly Leu Tyr Ser Leu Ser Ser Val Val 
                405                 410                 415     


Thr Val Pro Ser Ser Ser Leu Gly Thr Lys Thr Tyr Thr Cys Asn Val 
            420                 425                 430         


Asp His Lys Pro Ser Asn Thr Lys Val Asp Lys Arg Val Glu Ser Lys 
        435                 440                 445             


Tyr Gly Pro Pro Cys Pro Pro Cys Pro Ala Pro Glu Phe Leu Gly Gly 
    450                 455                 460                 


Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile 
465                 470                 475                 480 


Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser Gln Glu 
                485                 490                 495     


Asp Pro Glu Val Gln Phe Asn Trp Tyr Val Asp Gly Val Glu Val His 
            500                 505                 510         


Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Phe Asn Ser Thr Tyr Arg 
        515                 520                 525             


Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys 
    530                 535                 540                 


Glu Tyr Lys Cys Lys Val Ser Asn Lys Gly Leu Pro Ser Ser Ile Glu 
545                 550                 555                 560 


Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr 
                565                 570                 575     


Thr Leu Pro Pro Ser Gln Glu Glu Met Thr Lys Asn Gln Val Ser Leu 
            580                 585                 590         


Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp 
        595                 600                 605             


Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val 
    610                 615                 620                 


Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Arg Leu Thr Val Asp 
625                 630                 635                 640 


Lys Ser Arg Trp Gln Glu Gly Asn Val Phe Ser Cys Ser Val Met His 
                645                 650                 655     


Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Leu 
            660                 665                 670         


Gly Lys 
        


<210>  32
<211>  2022
<212>  DNA
<213>  Artificial

<220>
<223>  609-Fab-PDL1-IgG4ĺ

<400>  32
gaggtcaagc tggtggaaag cggcggcggc ctggtgcagc ctggaggatc cctgcggctg       60

agctgcgctg cctccggctt cgctttcagc tcctatgaca tgtcctgggt gaggcaggcc      120

cctggaaaga ggctggagtg ggtggctacc atctccggag gcggaaggta cacctactac      180

cccgacacag tgaagggaag gttcaccatc agccgggata acgccaaaaa cagccactat      240

ctccagatga actccctgag ggccgaagat acagccgtgt atttctgtgc ctccccctac      300

ggaggctatt ttgacgtgtg gggacagggc accctggtga ccgtctcctc cgcaagtacc      360

aagggaccta gtgttttccc tcttgcacct tgctccaggt caacatcaga gtccacagct      420

gctcttggat gtctcgttaa ggactacttc ccagagccag ttaccgtatc ctggaactcc      480

ggagctttga caagcggcgt tcatacattc ccagctgtgt tgcagagttc tgggttgtac      540

agtttgagct cagtggtgac cgtgccttca tcttctttgg gcactaagac ctacacctgc      600

aacgtggatc acaagccaag caacaccaag gtggataaga gggtgggtgg aggcggttca      660

ggcggaggtg gcagcggagg tggcgggagt caggtccagc tgcagcagtc aggagggggc      720

ctggtgaagc catcacagag cctgtccctg acctgcacag tctctgggtt cagtctgact      780

tcatacggag tgcactgggt ccgacagccc cctggaaagg gactggagtg gatcggcctg      840

atttggtctg gcgggggaac agactataac cccagcctga aatcccggct gaccatctct      900

agagatacca gtaagaatca agtgagcttt aaaattagct ccctgacagc cgctgacact      960

gcagtgtact attgtgcaag gcagctggga ctgcgagcta tggattactg gggacagggc     1020

acttccgtga ccgtctctag tgcaagtacc aagggaccta gtgttttccc tcttgcacct     1080

tgctccaggt caacatcaga gtccacagct gctcttggat gtctcgttaa ggactacttc     1140

ccagagccag ttaccgtatc ctggaactcc ggagctttga caagcggcgt tcatacattc     1200

ccagctgtgt tgcagagttc tgggttgtac agtttgagct cagtggtgac cgtgccttca     1260

tcttctttgg gcactaagac ctacacctgc aacgtggatc acaagccaag caacaccaag     1320

gtggataaga gggtggagtc caagtacggc ccaccatgtc ctccatgtcc agcccctgaa     1380

tttttgggcg ggccttctgt ctttctgttt cctcctaaac ctaaagatac cctgatgatc     1440

agccgcacac ccgaagtcac ttgtgtggtc gtggatgtgt ctcaggaaga tcccgaagtg     1500

cagtttaact ggtatgtcga tggcgtggaa gtgcataatg ccaaaactaa gccccgcgaa     1560

gaacagttca acagcactta tcgggtcgtg tctgtgctca cagtcctcca tcaggattgg     1620

ctgaatggga aagaatataa gtgcaaggtg agcaataagg gcctccccag cagcatcgag     1680

aagactatta gcaaagccaa agggcagcca cgggaacccc aggtgtacac tctgcccccc     1740

tctcaggagg agatgactaa aaatcaggtc tctctgactt gtctggtgaa agggttttat     1800

cccagcgaca ttgccgtgga gtgggagtct aatggccagc ccgagaataa ttataagaca     1860

actccccccg tcctggactc tgacggcagc tttttcctgt attctcggct gacagtggac     1920

aaaagtcgct ggcaggaggg caatgtcttt agttgcagtg tcatgcatga ggccctgcac     1980

aatcactata cacagaaaag cctgtctctg agtctgggca aa                        2022


