                         SEQUENCE LISTING

<110>  Ϻ＼޹˾
 
<120>  CD73弰;

<130>  IEC200316PCT

<150>  PCT/CN2020/089869
<151>  2020-05-12

<160>  21    

<170>  PatentIn version 3.5

<210>  1
<211>  117
<212>  PRT
<213>  ˹

<220>
<223>  72G8ɱ

<400>  1

Glu Val Arg Leu Gln Gln Ser Gly Ala Glu Leu Val Lys Pro Gly Ala 
1               5                   10                  15      


Ser Val Lys Met Ser Cys Lys Ala Ser Gly Tyr Ser Leu Thr Thr Tyr 
            20                  25                  30          


Pro Ile Glu Trp Met Lys Gln Asn His Gly Lys Ser Leu Glu Trp Ile 
        35                  40                  45              


Gly Asn Phe His Pro Tyr Asn Asn Asp Thr Lys Phe Asn Glu Lys Phe 
    50                  55                  60                  


Arg Gly Lys Ala Thr Met Thr Val Glu Lys Ser Ser Asn Thr Val Tyr 
65                  70                  75                  80  


Leu Glu Leu Ser Arg Leu Thr Ser Asp Asp Ser Ala Val Tyr Tyr Cys 
                85                  90                  95      


Thr Arg Gly Asp Tyr Tyr Gly Asn Ser Asp Tyr Trp Gly Gln Gly Thr 
            100                 105                 110         


Thr Leu Thr Val Ser 
        115         


<210>  2
<211>  107
<212>  PRT
<213>  ˹

<220>
<223>  72G8ɱ

<400>  2

Asp Ile Gln Met Thr Gln Thr Thr Ser Ser Leu Ser Ala Ser Leu Gly 
1               5                   10                  15      


Asp Arg Val Thr Ile Ser Cys Arg Ala Ser Gln Asp Ile Ser Asn Tyr 
            20                  25                  30          


Leu Asn Trp Tyr Gln Gln Lys Pro Asp Gly Thr Val Lys Leu Leu Ile 
        35                  40                  45              


Tyr Tyr Thr Ser Arg Leu His Ser Gly Val Pro Ala Arg Phe Arg Gly 
    50                  55                  60                  


Ser Gly Ser Gly Thr Asp Tyr Ser Leu Thr Ile Ser Asn Leu Gln Gln 
65                  70                  75                  80  


Glu Asp Phe Ala Thr Tyr Phe Cys Gln Gln Gly Asn Thr Leu Pro Tyr 
                85                  90                  95      


Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile Lys 
            100                 105         


<210>  3
<211>  7
<212>  PRT
<213>  ˹

<220>
<223>  HCDR1

<400>  3

Gly Tyr Ser Leu Thr Thr Tyr 
1               5           


<210>  4
<211>  6
<212>  PRT
<213>  ˹

<220>
<223>  HCDR2-1

<400>  4

His Pro Tyr Asn Asn Asp 
1               5       


<210>  5
<211>  8
<212>  PRT
<213>  ˹

<220>
<223>  HCDR3

<400>  5

Gly Asp Tyr Tyr Gly Asn Ser Asp 
1               5               


<210>  6
<211>  11
<212>  PRT
<213>  ˹

<220>
<223>  LCDR1

<400>  6

Arg Ala Ser Gln Asp Ile Ser Asn Tyr Leu Asn 
1               5                   10      


<210>  7
<211>  7
<212>  PRT
<213>  ˹

<220>
<223>  LCDR2

<400>  7

Tyr Thr Ser Arg Leu His Ser 
1               5           


<210>  8
<211>  9
<212>  PRT
<213>  ˹

<220>
<223>  LCDR3

<400>  8

Gln Gln Gly Asn Thr Leu Pro Tyr Thr 
1               5                   


<210>  9
<211>  118
<212>  PRT
<213>  ˹

<220>
<223>  7002-04ɱ

<400>  9

Gln Val Gln Leu Val Gln Ser Gly Ala Glu Val Val Lys Pro Gly Ala 
1               5                   10                  15      


Ser Val Lys Met Ser Cys Lys Ala Ser Gly Tyr Ser Leu Thr Thr Tyr 
            20                  25                  30          


Pro Ile Glu Trp Met Lys Gln Ala His Gly Gln Gly Leu Glu Trp Ile 
        35                  40                  45              


Gly Asn Phe His Pro Tyr Asn Asn Asp Thr Lys Phe Asn Glu Lys Phe 
    50                  55                  60                  


Arg Gly Lys Ala Thr Met Thr Val Asp Lys Ser Ile Asn Thr Val Tyr 
65                  70                  75                  80  


Leu Glu Leu Ser Arg Leu Arg Ser Asp Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Thr Arg Gly Asp Tyr Tyr Gly Asn Ser Asp Tyr Trp Gly Gln Gly Thr 
            100                 105                 110         


Leu Leu Thr Val Ser Ser 
        115             


<210>  10
<211>  107
<212>  PRT
<213>  ˹

<220>
<223>  7002-04ɱ

<400>  10

Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly 
1               5                   10                  15      


Asp Arg Val Thr Ile Thr Cys Arg Ala Ser Gln Asp Ile Ser Asn Tyr 
            20                  25                  30          


Leu Asn Trp Tyr Gln Gln Lys Pro Gly Lys Ala Val Lys Leu Leu Ile 
        35                  40                  45              


Tyr Tyr Thr Ser Arg Leu His Ser Gly Val Pro Ser Arg Phe Ser Gly 
    50                  55                  60                  


Ser Gly Ser Gly Thr Asp Tyr Thr Leu Thr Ile Ser Ser Leu Gln Pro 
65                  70                  75                  80  


Glu Asp Phe Ala Thr Tyr Phe Cys Gln Gln Gly Asn Thr Leu Pro Tyr 
                85                  90                  95      


Thr Phe Gly Gln Gly Thr Lys Leu Glu Ile Lys 
            100                 105         


<210>  11
<211>  6
<212>  PRT
<213>  ˹

<220>
<223>  HCDR2-2

<400>  11

His Pro Tyr Asn Gln Asp 
1               5       


<210>  12
<211>  117
<212>  PRT
<213>  ˹

<220>
<223>  72G8-N56Qɱ

<400>  12

Glu Val Arg Leu Gln Gln Ser Gly Ala Glu Leu Val Lys Pro Gly Ala 
1               5                   10                  15      


Ser Val Lys Met Ser Cys Lys Ala Ser Gly Tyr Ser Leu Thr Thr Tyr 
            20                  25                  30          


Pro Ile Glu Trp Met Lys Gln Asn His Gly Lys Ser Leu Glu Trp Ile 
        35                  40                  45              


Gly Asn Phe His Pro Tyr Asn Gln Asp Thr Lys Phe Asn Glu Lys Phe 
    50                  55                  60                  


Arg Gly Lys Ala Thr Met Thr Val Glu Lys Ser Ser Asn Thr Val Tyr 
65                  70                  75                  80  


Leu Glu Leu Ser Arg Leu Thr Ser Asp Asp Ser Ala Val Tyr Tyr Cys 
                85                  90                  95      


Thr Arg Gly Asp Tyr Tyr Gly Asn Ser Asp Tyr Trp Gly Gln Gly Thr 
            100                 105                 110         


Thr Leu Thr Val Ser 
        115         


<210>  13
<211>  118
<212>  PRT
<213>  ˹

<220>
<223>  7002-04-N56Qɱ

<400>  13

Gln Val Gln Leu Val Gln Ser Gly Ala Glu Val Val Lys Pro Gly Ala 
1               5                   10                  15      


Ser Val Lys Met Ser Cys Lys Ala Ser Gly Tyr Ser Leu Thr Thr Tyr 
            20                  25                  30          


Pro Ile Glu Trp Met Lys Gln Ala His Gly Gln Gly Leu Glu Trp Ile 
        35                  40                  45              


Gly Asn Phe His Pro Tyr Asn Gln Asp Thr Lys Phe Asn Glu Lys Phe 
    50                  55                  60                  


Arg Gly Lys Ala Thr Met Thr Val Asp Lys Ser Ile Asn Thr Val Tyr 
65                  70                  75                  80  


Leu Glu Leu Ser Arg Leu Arg Ser Asp Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Thr Arg Gly Asp Tyr Tyr Gly Asn Ser Asp Tyr Trp Gly Gln Gly Thr 
            100                 105                 110         


Leu Leu Thr Val Ser Ser 
        115             


<210>  14
<211>  351
<212>  DNA
<213>  ˹

<220>
<223>  72G8ɱ

<400>  14
gaggtgcggc tgcagcagtc tggggctgag ctggtgaagc ctggggcctc agtgaagatg       60

tcctgcaagg cttctggcta ctccctcacc acctacccca tcgaatggat gaagcagaac      120

catggcaaga gccttgagtg gattggaaac tttcatcctt ataataatga tacaaagttc      180

aacgagaagt ttaggggcaa ggccaccatg accgtagaaa agtccagcaa cacagtgtac      240

ctggagctga gcaggctgac ctctgacgac tcggccgtgt attactgtac gagaggggat      300

tactacggca atagtgacta ctggggccaa gggaccacgc tcaccgtctc c               351


<210>  15
<211>  321
<212>  DNA
<213>  ˹

<220>
<223>  72G8ɱ

<400>  15
gacatccaga tgacccagac tacctcctcc ctgtctgcat ctctgggaga cagagtcacc       60

atctcctgcc gggcaagtca ggacattagc aactatttaa attggtatca gcagaaacca      120

gacggaaccg ttaagctcct gatctattat acatccaggt tgcacagtgg ggtcccagcc      180

agattccgtg gcagtggatc tgggacagat tactccctca ccatcagcaa cctgcaacag      240

gaagattttg caacttactt ctgtcaacag ggtaacactc tcccttacac tttcggcgga      300

gggaccaagc tggagatcaa a                                                321


<210>  16
<211>  354
<212>  DNA
<213>  ˹

<220>
<223>  7002-04ɱ

<400>  16
caggtgcagc tggtgcagag cggcgccgag gtggtgaagc ccggagcttc cgtgaagatg       60

agctgcaagg ccagcggcta cagcctgaca acatacccca tcgagtggat gaagcaggcc      120

cacggccagg gactggagtg gatcggaaat tttcatccct ataataacga caccaagttc      180

aacgagaagt tcagaggcaa ggccaccatg accgtggaca agagcatcaa caccgtgtat      240

ctggagctga gcagactgag aagcgacgac accgccgtgt actactgcac cagaggcgac      300

tactacggca acagcgacta ctggggccag ggcacactgc tgaccgtgag ctcc            354


<210>  17
<211>  321
<212>  DNA
<213>  ˹

<220>
<223>  7002-04ɱ

<400>  17
gacattcaga tgacccagag ccccagcagc ctgagcgcca gcgtgggaga cagagtgacc       60

atcacctgca gagccagcca ggacatctcc aactacctga actggtacca gcagaaaccc      120

ggcaaagccg tgaagctgct gatctactac accagcagac tgcacagcgg cgtgcccagc      180

agattcagcg gaagcggcag cggcaccgac tacaccctga ctatcagctc cctgcagccc      240

gaagatttcg ccacctactt ctgccagcag ggcaacaccc tgccctatac attcggacag      300

ggcaccaagc tggagatcaa a                                                321


<210>  18
<211>  354
<212>  DNA
<213>  ˹

<220>
<223>  7002-04-N56Qɱ

<400>  18
caggtgcagc tggtgcagag cggcgccgag gtggtgaagc ccggagcttc cgtgaagatg       60

agctgcaagg ccagcggcta cagcctgaca acatacccca tcgagtggat gaagcaggcc      120

cacggccagg gactggagtg gatcggaaat tttcatccct ataatcagga caccaagttc      180

aacgagaagt tcagaggcaa ggccaccatg accgtggaca agagcatcaa caccgtgtat      240

ctggagctga gcagactgag aagcgacgac accgccgtgt actactgcac cagaggcgac      300

tactacggca acagcgacta ctggggccag ggcacactgc tgaccgtgag ctcc            354


<210>  19
<211>  330
<212>  PRT
<213>  ˹

<220>
<223>  IgG1-TM㶨

<400>  19

Ala Ser Thr Lys Gly Pro Ser Val Phe Pro Leu Ala Pro Ser Ser Lys 
1               5                   10                  15      


Ser Thr Ser Gly Gly Thr Ala Ala Leu Gly Cys Leu Val Lys Asp Tyr 
            20                  25                  30          


Phe Pro Glu Pro Val Thr Val Ser Trp Asn Ser Gly Ala Leu Thr Ser 
        35                  40                  45              


Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Ser Gly Leu Tyr Ser 
    50                  55                  60                  


Leu Ser Ser Val Val Thr Val Pro Ser Ser Ser Leu Gly Thr Gln Thr 
65                  70                  75                  80  


Tyr Ile Cys Asn Val Asn His Lys Pro Ser Asn Thr Lys Val Asp Lys 
                85                  90                  95      


Lys Val Glu Pro Lys Ser Cys Asp Lys Thr His Thr Cys Pro Pro Cys 
            100                 105                 110         


Pro Ala Pro Glu Phe Glu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro 
        115                 120                 125             


Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys 
    130                 135                 140                 


Val Val Val Asp Val Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp 
145                 150                 155                 160 


Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu 
                165                 170                 175     


Glu Gln Tyr Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu 
            180                 185                 190         


His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn 
        195                 200                 205             


Lys Ala Leu Pro Ala Ser Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly 
    210                 215                 220                 


Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Glu Glu 
225                 230                 235                 240 


Met Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr 
                245                 250                 255     


Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn 
            260                 265                 270         


Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe 
        275                 280                 285             


Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn 
    290                 295                 300                 


Val Phe Ser Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr 
305                 310                 315                 320 


Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys 
                325                 330 


<210>  20
<211>  107
<212>  PRT
<213>  ˹

<220>
<223>  ˦㶨

<400>  20

Arg Thr Val Ala Ala Pro Ser Val Phe Ile Phe Pro Pro Ser Asp Glu 
1               5                   10                  15      


Gln Leu Lys Ser Gly Thr Ala Ser Val Val Cys Leu Leu Asn Asn Phe 
            20                  25                  30          


Tyr Pro Arg Glu Ala Lys Val Gln Trp Lys Val Asp Asn Ala Leu Gln 
        35                  40                  45              


Ser Gly Asn Ser Gln Glu Ser Val Thr Glu Gln Asp Ser Lys Asp Ser 
    50                  55                  60                  


Thr Tyr Ser Leu Ser Ser Thr Leu Thr Leu Ser Lys Ala Asp Tyr Glu 
65                  70                  75                  80  


Lys His Lys Val Tyr Ala Cys Glu Val Thr His Gln Gly Leu Ser Ser 
                85                  90                  95      


Pro Val Thr Lys Ser Phe Asn Arg Gly Glu Cys 
            100                 105         


<210>  21
<211>  574
<212>  PRT
<213>  ˹

<220>
<223>  CD73

<400>  21

Met Cys Pro Arg Ala Ala Arg Ala Pro Ala Thr Leu Leu Leu Ala Leu 
1               5                   10                  15      


Gly Ala Val Leu Trp Pro Ala Ala Gly Ala Trp Glu Leu Thr Ile Leu 
            20                  25                  30          


His Thr Asn Asp Val His Ser Arg Leu Glu Gln Thr Ser Glu Asp Ser 
        35                  40                  45              


Ser Lys Cys Val Asn Ala Ser Arg Cys Met Gly Gly Val Ala Arg Leu 
    50                  55                  60                  


Phe Thr Lys Val Gln Gln Ile Arg Arg Ala Glu Pro Asn Val Leu Leu 
65                  70                  75                  80  


Leu Asp Ala Gly Asp Gln Tyr Gln Gly Thr Ile Trp Phe Thr Val Tyr 
                85                  90                  95      


Lys Gly Ala Glu Val Ala His Phe Met Asn Ala Leu Arg Tyr Asp Ala 
            100                 105                 110         


Met Ala Leu Gly Asn His Glu Phe Asp Asn Gly Val Glu Gly Leu Ile 
        115                 120                 125             


Glu Pro Leu Leu Lys Glu Ala Lys Phe Pro Ile Leu Ser Ala Asn Ile 
    130                 135                 140                 


Lys Ala Lys Gly Pro Leu Ala Ser Gln Ile Ser Gly Leu Tyr Leu Pro 
145                 150                 155                 160 


Tyr Lys Val Leu Pro Val Gly Asp Glu Val Val Gly Ile Val Gly Tyr 
                165                 170                 175     


Thr Ser Lys Glu Thr Pro Phe Leu Ser Asn Pro Gly Thr Asn Leu Val 
            180                 185                 190         


Phe Glu Asp Glu Ile Thr Ala Leu Gln Pro Glu Val Asp Lys Leu Lys 
        195                 200                 205             


Thr Leu Asn Val Asn Lys Ile Ile Ala Leu Gly His Ser Gly Phe Glu 
    210                 215                 220                 


Met Asp Lys Leu Ile Ala Gln Lys Val Arg Gly Val Asp Val Val Val 
225                 230                 235                 240 


Gly Gly His Ser Asn Thr Phe Leu Tyr Thr Gly Asn Pro Pro Ser Lys 
                245                 250                 255     


Glu Val Pro Ala Gly Lys Tyr Pro Phe Ile Val Thr Ser Asp Asp Gly 
            260                 265                 270         


Arg Lys Val Pro Val Val Gln Ala Tyr Ala Phe Gly Lys Tyr Leu Gly 
        275                 280                 285             


Tyr Leu Lys Ile Glu Phe Asp Glu Arg Gly Asn Val Ile Ser Ser His 
    290                 295                 300                 


Gly Asn Pro Ile Leu Leu Asn Ser Ser Ile Pro Glu Asp Pro Ser Ile 
305                 310                 315                 320 


Lys Ala Asp Ile Asn Lys Trp Arg Ile Lys Leu Asp Asn Tyr Ser Thr 
                325                 330                 335     


Gln Glu Leu Gly Lys Thr Ile Val Tyr Leu Asp Gly Ser Ser Gln Ser 
            340                 345                 350         


Cys Arg Phe Arg Glu Cys Asn Met Gly Asn Leu Ile Cys Asp Ala Met 
        355                 360                 365             


Ile Asn Asn Asn Leu Arg His Thr Asp Glu Met Phe Trp Asn His Val 
    370                 375                 380                 


Ser Met Cys Ile Leu Asn Gly Gly Gly Ile Arg Ser Pro Ile Asp Glu 
385                 390                 395                 400 


Arg Asn Asn Gly Thr Ile Thr Trp Glu Asn Leu Ala Ala Val Leu Pro 
                405                 410                 415     


Phe Gly Gly Thr Phe Asp Leu Val Gln Leu Lys Gly Ser Thr Leu Lys 
            420                 425                 430         


Lys Ala Phe Glu His Ser Val His Arg Tyr Gly Gln Ser Thr Gly Glu 
        435                 440                 445             


Phe Leu Gln Val Gly Gly Ile His Val Val Tyr Asp Leu Ser Arg Lys 
    450                 455                 460                 


Pro Gly Asp Arg Val Val Lys Leu Asp Val Leu Cys Thr Lys Cys Arg 
465                 470                 475                 480 


Val Pro Ser Tyr Asp Pro Leu Lys Met Asp Glu Val Tyr Lys Val Ile 
                485                 490                 495     


Leu Pro Asn Phe Leu Ala Asn Gly Gly Asp Gly Phe Gln Met Ile Lys 
            500                 505                 510         


Asp Glu Leu Leu Arg His Asp Ser Gly Asp Gln Asp Ile Asn Val Val 
        515                 520                 525             


Ser Thr Tyr Ile Ser Lys Met Lys Val Ile Tyr Pro Ala Val Glu Gly 
    530                 535                 540                 


Arg Ile Lys Phe Ser Thr Gly Ser His Cys His Gly Ser Phe Ser Leu 
545                 550                 555                 560 


Ile Phe Leu Ser Leu Trp Ala Val Ile Phe Val Leu Tyr Gln 
                565                 570                 


