                         SEQUENCE LISTING

<110>  The Wistar Institute of Anatomy and Biology
       Muthumani, Kar
       Weiner, David
 
<120>  DNA ANTIBODY CONSTRUCTS FOR USE AGAINST HEPATITIS B VIRUS

<130>  206193-0039-00WO

<150>  US 63/021,189
<151>  2020-05-07

<160>  2     

<170>  PatentIn version 3.5

<210>  1
<211>  2211
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Optimized DMAb DNA Sequence

<400>  1
atggactgga cttggaggat tctgtttctg gtcgctgctg ctaccgggac tcacgccgag       60

gtgcaggtgc tggagagcgg gggagggctg gtgcagccag gcggcagcct gaggctgtcc      120

tgcgcagcat ctggcttcag gttcagcagc tacgccatgt cctgggtgcg gcaggcacca      180

ggcaagggcc tggagtgggt gtccggcatc tctggcaccg gcgagaacac atactatgcc      240

gacagcgtga agggcaggtt taccatcagc agagataact ccaagaatac actgtacgtg      300

cagatgaatt ctctgcgggc cgaggacacc gccgtgtact attgcgcaaa ggatgcaatc      360

ctgggcagcg gacacccatg gtattttcac gtgtggggaa ggggcaccct ggtgacagtg      420

tctagcgcct ccacaaaggg acctagcgtg ttcccactgg caccctcctc taagtccacc      480

tctggcggca cagccgccct gggctgtctg gtgaaggact acttccccga gcctgtgacc      540

gtgagctgga actccggcgc cctgaccagc ggagtgcaca catttcctgc cgtgctgcag      600

agctccggcc tgtactccct gtctagcgtg gtgaccgtgc catcctctag cctgggcaca      660

cagacctata tctgcaacgt gaatcacaag cctagcaata caaaggtgga caagaaggtg      720

gagccaaagt cctgtgataa gacacacacc tgccctccct gtccagcacc agagctgctg      780

ggcggcccat ccgtgttcct gtttccaccc aagcctaagg acacactgat gatctctcgg      840

accccagagg tgacatgcgt ggtggtggac gtgagccacg aggaccccga ggtgaagttc      900

aactggtacg tggatggcgt ggaggtgcac aatgccaaga ccaagccccg ggaggagcag      960

tacaactcta cctatcgcgt ggtgagcgtg ctgacagtgc tgcaccagga ttggctgaac     1020

ggcaaggagt ataagtgcaa ggtgtccaat aaggccctgc ctgccccaat cgagaagacc     1080

atctctaagg ccaagggcca gcctagggag ccacaggtgt acacactgcc tccatccaga     1140

gacgagctga ccaagaacca ggtgtctctg acatgtctgg tgaagggctt ttatcccagc     1200

gatatcgccg tggagtggga gtccaatggc cagcctgaga acaattacaa gaccacaccc     1260

cctgtgctgg actctgatgg cagcttcttt ctgtattcca agctgaccgt ggacaagtct     1320

cgctggcagc agggcaacgt gttcagctgc tccgtgatgc acgaggccct gcacaatcac     1380

tacacccaga agtctctgag cctgtcccca ggcaagaggg gaaggaagag gagatctggc     1440

agcggcgcca caaacttttc cctgctgaag caggcaggcg atgtggagga gaatccagga     1500

cctatggtgc tgcagaccca ggtgttcatc agcctgctgc tgtggatcag cggcgcctac     1560

ggctcctatg tgctgacaca gccaccctcc gtgtctgtgg cacctggaca gaccgccagg     1620

atgacatgtg gcggaaacaa tatcggcagc gagtccgtgc actggtttca gcagaagcca     1680

ggacaggcac ctgtgctggt ggtgtatgac gattctgacc ggccaagcgg catccccgag     1740

aggttcagcg gcagcaactc cggcaataca gccaccctga caatcagcag agtggaggca     1800

ggcgacgagg cagattacta ttgccaagtg tgggactcct ctagcgatca cgccgtgttc     1860

ggcggcggaa cccagctgac agtgctggga cagcctaagg cagcaccatc cgtgaccctg     1920

tttcctccat cctctgagga gctgcaggcc aacaaggcca ccctggtgtg cctgatcagc     1980

gacttctacc ctggagcagt gacagtggca tggaaggccg atagctcccc agtgaaggcc     2040

ggcgtggaga caacaacccc ctccaagcag tctaacaata agtacgccgc ctctagctat     2100

ctgtctctga ccccagagca gtggaagagc cacaagtctt atagctgcca ggtcactcac     2160

gaaggctcaa ctgtggaaaa aaccgtcgct cctaccgaat gttcttgata a              2211


<210>  2
<211>  735
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Optimized DMAb Amino Acid Sequence

<400>  2

Met Asp Trp Thr Trp Arg Ile Leu Phe Leu Val Ala Ala Ala Thr Gly 
1               5                   10                  15      


Thr His Ala Glu Val Gln Val Leu Glu Ser Gly Gly Gly Leu Val Gln 
            20                  25                  30          


Pro Gly Gly Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Arg Phe 
        35                  40                  45              


Ser Ser Tyr Ala Met Ser Trp Val Arg Gln Ala Pro Gly Lys Gly Leu 
    50                  55                  60                  


Glu Trp Val Ser Gly Ile Ser Gly Thr Gly Glu Asn Thr Tyr Tyr Ala 
65                  70                  75                  80  


Asp Ser Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn 
                85                  90                  95      


Thr Leu Tyr Val Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val 
            100                 105                 110         


Tyr Tyr Cys Ala Lys Asp Ala Ile Leu Gly Ser Gly His Pro Trp Tyr 
        115                 120                 125             


Phe His Val Trp Gly Arg Gly Thr Leu Val Thr Val Ser Ser Ala Ser 
    130                 135                 140                 


Thr Lys Gly Pro Ser Val Phe Pro Leu Ala Pro Ser Ser Lys Ser Thr 
145                 150                 155                 160 


Ser Gly Gly Thr Ala Ala Leu Gly Cys Leu Val Lys Asp Tyr Phe Pro 
                165                 170                 175     


Glu Pro Val Thr Val Ser Trp Asn Ser Gly Ala Leu Thr Ser Gly Val 
            180                 185                 190         


His Thr Phe Pro Ala Val Leu Gln Ser Ser Gly Leu Tyr Ser Leu Ser 
        195                 200                 205             


Ser Val Val Thr Val Pro Ser Ser Ser Leu Gly Thr Gln Thr Tyr Ile 
    210                 215                 220                 


Cys Asn Val Asn His Lys Pro Ser Asn Thr Lys Val Asp Lys Lys Val 
225                 230                 235                 240 


Glu Pro Lys Ser Cys Asp Lys Thr His Thr Cys Pro Pro Cys Pro Ala 
                245                 250                 255     


Pro Glu Leu Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro 
            260                 265                 270         


Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val 
        275                 280                 285             


Val Asp Val Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr Val 
    290                 295                 300                 


Asp Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln 
305                 310                 315                 320 


Tyr Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln 
                325                 330                 335     


Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala 
            340                 345                 350         


Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro 
        355                 360                 365             


Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Asp Glu Leu Thr 
    370                 375                 380                 


Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser 
385                 390                 395                 400 


Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr 
                405                 410                 415     


Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr 
            420                 425                 430         


Ser Lys Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe 
        435                 440                 445             


Ser Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys 
    450                 455                 460                 


Ser Leu Ser Leu Ser Pro Gly Lys Arg Gly Arg Lys Arg Arg Ser Gly 
465                 470                 475                 480 


Ser Gly Ala Thr Asn Phe Ser Leu Leu Lys Gln Ala Gly Asp Val Glu 
                485                 490                 495     


Glu Asn Pro Gly Pro Met Val Leu Gln Thr Gln Val Phe Ile Ser Leu 
            500                 505                 510         


Leu Leu Trp Ile Ser Gly Ala Tyr Gly Ser Tyr Val Leu Thr Gln Pro 
        515                 520                 525             


Pro Ser Val Ser Val Ala Pro Gly Gln Thr Ala Arg Met Thr Cys Gly 
    530                 535                 540                 


Gly Asn Asn Ile Gly Ser Glu Ser Val His Trp Phe Gln Gln Lys Pro 
545                 550                 555                 560 


Gly Gln Ala Pro Val Leu Val Val Tyr Asp Asp Ser Asp Arg Pro Ser 
                565                 570                 575     


Gly Ile Pro Glu Arg Phe Ser Gly Ser Asn Ser Gly Asn Thr Ala Thr 
            580                 585                 590         


Leu Thr Ile Ser Arg Val Glu Ala Gly Asp Glu Ala Asp Tyr Tyr Cys 
        595                 600                 605             


Gln Val Trp Asp Ser Ser Ser Asp His Ala Val Phe Gly Gly Gly Thr 
    610                 615                 620                 


Gln Leu Thr Val Leu Gly Gln Pro Lys Ala Ala Pro Ser Val Thr Leu 
625                 630                 635                 640 


Phe Pro Pro Ser Ser Glu Glu Leu Gln Ala Asn Lys Ala Thr Leu Val 
                645                 650                 655     


Cys Leu Ile Ser Asp Phe Tyr Pro Gly Ala Val Thr Val Ala Trp Lys 
            660                 665                 670         


Ala Asp Ser Ser Pro Val Lys Ala Gly Val Glu Thr Thr Thr Pro Ser 
        675                 680                 685             


Lys Gln Ser Asn Asn Lys Tyr Ala Ala Ser Ser Tyr Leu Ser Leu Thr 
    690                 695                 700                 


Pro Glu Gln Trp Lys Ser His Lys Ser Tyr Ser Cys Gln Val Thr His 
705                 710                 715                 720 


Glu Gly Ser Thr Val Glu Lys Thr Val Ala Pro Thr Glu Cys Ser 
                725                 730                 735 


