                         SEQUENCE LISTING

<110>  Inhibrx, Inc.
 
<120>  CANINE PD-1-BINDING POLYPEPTIDES AND USES THEREOF

<130>  01202-0016-00PCT

<150>  US 63/019,817
<151>  2020-05-04

<160>  31    

<170>  PatentIn version 3.5

<210>  1
<211>  288
<212>  PRT
<213>  Canis familiaris

<400>  1

Met Gly Ser Arg Arg Gly Pro Trp Pro Leu Val Trp Ala Val Leu Gln 
1               5                   10                  15      


Leu Gly Trp Trp Pro Gly Trp Leu Leu Asp Ser Pro Asp Arg Pro Trp 
            20                  25                  30          


Ser Pro Leu Thr Phe Ser Pro Ala Gln Leu Thr Val Gln Glu Gly Glu 
        35                  40                  45              


Asn Ala Thr Phe Thr Cys Ser Leu Ala Asp Ile Pro Asp Ser Phe Val 
    50                  55                  60                  


Leu Asn Trp Tyr Arg Leu Ser Pro Arg Asn Gln Thr Asp Lys Leu Ala 
65                  70                  75                  80  


Ala Phe Gln Glu Asp Arg Ile Glu Pro Gly Arg Asp Arg Arg Phe Arg 
                85                  90                  95      


Val Thr Arg Leu Pro Asn Gly Arg Asp Phe His Met Ser Ile Val Ala 
            100                 105                 110         


Ala Arg Leu Asn Asp Ser Gly Ile Tyr Leu Cys Gly Ala Ile Tyr Leu 
        115                 120                 125             


Pro Pro Asn Thr Gln Ile Asn Glu Ser Pro Arg Ala Glu Leu Ser Val 
    130                 135                 140                 


Thr Glu Arg Thr Leu Glu Pro Pro Thr Gln Ser Pro Ser Pro Pro Pro 
145                 150                 155                 160 


Arg Leu Ser Gly Gln Leu Gln Gly Leu Val Ile Gly Val Thr Ser Val 
                165                 170                 175     


Leu Val Gly Val Leu Leu Leu Leu Leu Leu Thr Trp Val Leu Ala Ala 
            180                 185                 190         


Val Phe Pro Arg Ala Thr Arg Gly Ala Cys Val Cys Gly Ser Glu Asp 
        195                 200                 205             


Glu Pro Leu Lys Glu Gly Pro Asp Ala Ala Pro Val Phe Thr Leu Asp 
    210                 215                 220                 


Tyr Gly Glu Leu Asp Phe Gln Trp Arg Glu Lys Thr Pro Glu Pro Pro 
225                 230                 235                 240 


Ala Pro Cys Ala Pro Glu Gln Thr Glu Tyr Ala Thr Ile Val Phe Pro 
                245                 250                 255     


Gly Arg Pro Ala Ser Pro Gly Arg Arg Ala Ser Ala Ser Ser Leu Gln 
            260                 265                 270         


Gly Ala Gln Pro Pro Ser Pro Glu Asp Gly Pro Gly Leu Trp Pro Leu 
        275                 280                 285             


<210>  2
<211>  123
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  PD1-8 VHH

<400>  2

Gln Val Gln Leu Gln Gln Ser Gly Gly Gly Leu Val Gln Ala Gly Gly 
1               5                   10                  15      


Ser Leu Arg Val Ser Cys Ala Ala Ser Gly Arg Thr Phe Ser Ser Tyr 
            20                  25                  30          


Gly Met Gly Trp Phe Arg Gln Ala Pro Gly Lys Glu Arg Glu Phe Val 
        35                  40                  45              


Ala Ala Ile Ser Trp Ser Gly Gly Thr Gln Tyr Tyr Ala Asp Ser Ala 
    50                  55                  60                  


Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys Asn Thr Val Tyr 
65                  70                  75                  80  


Leu Gln Met Asn Ser Leu Lys Pro Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Asp Tyr Thr Asp Tyr Val Val Tyr Arg Pro Gln Glu Ile Gly Tyr 
            100                 105                 110         


Trp Gly Gln Gly Thr Gln Val Thr Val Lys Pro 
        115                 120             


<210>  3
<211>  120
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  PD1-14 VHH

<400>  3

Gln Val Gln Leu Gln Gln Ser Gly Gly Gly Leu Ala Gln Ala Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Arg Thr Val Ser Ile Tyr 
            20                  25                  30          


Ala Met Gly Trp Phe Arg Gln Ala Pro Gly Lys Glu Arg Glu Phe Val 
        35                  40                  45              


Ala Gly Ile Gly Trp Asn Gly Gly Thr Thr Tyr Tyr Ala Asp Ser Val 
    50                  55                  60                  


Glu Gly Arg Phe Thr Ile Ser Arg Asp His Ala Lys Asn Thr Ala Tyr 
65                  70                  75                  80  


Leu Gln Met Asn Ser Leu Lys Pro Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Ala Gln Glu Ser Ala Ala Gly Thr Leu Gly Asp Tyr Trp Gly Arg 
            100                 105                 110         


Gly Thr Gln Val Thr Val Lys Pro 
        115                 120 


<210>  4
<211>  120
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  PD1-13 VHH

<400>  4

Gln Val Thr Leu Lys Glu Ser Gly Gly Gly Leu Val Gln Ala Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Arg Thr Glu Ile Ile Tyr 
            20                  25                  30          


Ala Met Gly Trp Phe Arg Gln Ala Pro Gly Lys Glu Arg Glu Phe Val 
        35                  40                  45              


Ala Gly Ile Gly Trp Ser Gly Gly Thr Thr Tyr Tyr Ala Asp Ser Val 
    50                  55                  60                  


Glu Gly Arg Phe Thr Ile Ser Arg Asp Ser Ala Lys Asn Thr Val Tyr 
65                  70                  75                  80  


Leu Gln Met Arg Ser Leu Lys Pro Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Ala Gln Asp Ser Val Ala Gly Thr Leu Gly Asp Tyr Trp Gly Gln 
            100                 105                 110         


Gly Thr Gln Val Thr Val Lys Pro 
        115                 120 


<210>  5
<211>  127
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  PD1-5 VHH

<400>  5

Gln Val Thr Leu Arg Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Leu Thr Phe Gly Leu Tyr 
            20                  25                  30          


Ala Met Thr Trp Phe Arg Gln Ala Pro Gly Lys Asp Arg Glu Gly Ile 
        35                  40                  45              


Ser Cys Ile Ser Ser Ser Asp Gly Ser Thr Ile Tyr Ala Asp Ser Val 
    50                  55                  60                  


Lys Gly Arg Phe Thr Ala Ser Arg Asp Asn Ala Lys Asp Thr Met Tyr 
65                  70                  75                  80  


Leu Gln Met Asn Asn Leu Asn Pro Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Thr Asp Tyr Glu Thr Arg Cys Asp Tyr Gly Leu Arg Leu Arg Asp 
            100                 105                 110         


Arg Thr Ala Tyr Trp Gly Pro Gly Thr Gln Val Thr Val Lys Pro 
        115                 120                 125         


<210>  6
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  PD1-14 CDR1

<400>  6

Gly Arg Thr Val Ser Ile Tyr Ala Met Gly 
1               5                   10  


<210>  7
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  PD1-14 CDR2

<400>  7

Gly Ile Gly Trp Asn Gly Gly Thr Thr Tyr 
1               5                   10  


<210>  8
<211>  11
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  PD1-14 CDR3

<400>  8

Gln Glu Ser Ala Ala Gly Thr Leu Gly Asp Tyr 
1               5                   10      


<210>  9
<211>  120
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  czPD1-14v1 VHH

<400>  9

Glu Val Gln Leu Val Glu Ser Gly Gly Gly Glu Val Lys Pro Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Val Ala Ser Gly Arg Thr Val Ser Ile Tyr 
            20                  25                  30          


Ala Met Gly Trp Phe Arg Gln Ala Pro Gly Lys Glu Arg Glu Phe Val 
        35                  40                  45              


Ala Gly Ile Gly Trp Ser Gly Gly Thr Thr Tyr Tyr Ala Asp Ala Val 
    50                  55                  60                  


Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys Asn Thr Val Tyr 
65                  70                  75                  80  


Leu Gln Met Ser Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Ala Gln Glu Ser Ala Ala Gly Thr Leu Gly Asp Tyr Trp Gly Gln 
            100                 105                 110         


Gly Thr Gln Val Thr Val Lys Pro 
        115                 120 


<210>  10
<211>  120
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  czPD1-14v2 VHH

<400>  10

Glu Val Gln Leu Val Glu Ser Gly Gly Gly Glu Val Lys Pro Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Val Ala Ser Gly Arg Thr Val Ser Ile Tyr 
            20                  25                  30          


Ala Met Gly Trp Phe Arg Gln Ala Pro Gly Lys Glu Arg Glu Phe Val 
        35                  40                  45              


Ala Gly Ile Gly Trp Asn Gly Gly Thr Thr Tyr Tyr Ala Asp Ala Val 
    50                  55                  60                  


Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys Asn Thr Val Tyr 
65                  70                  75                  80  


Leu Gln Met Ser Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Ala Gln Glu Ser Ala Ala Gly Thr Leu Gly Asp Tyr Trp Gly Gln 
            100                 105                 110         


Gly Thr Gln Val Thr Val Lys Pro 
        115                 120 


<210>  11
<211>  120
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  czPD-1-14v3 VHH

<400>  11

Glu Val Gln Leu Val Glu Ser Gly Gly Gly Glu Val Lys Pro Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Val Ala Ser Gly Arg Thr Val Ser Ile Tyr 
            20                  25                  30          


Ala Met Gly Trp Phe Arg Gln Ala Pro Gly Lys Glu Arg Glu Phe Val 
        35                  40                  45              


Ala Gly Ile Gly Trp Asn Gly Gly Thr Thr Tyr Tyr Ala Asp Ala Val 
    50                  55                  60                  


Lys Gly Arg Phe Thr Ile Ser Arg Asp His Ala Lys Asn Thr Val Tyr 
65                  70                  75                  80  


Leu Gln Met Ser Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Ala Gln Glu Ser Ala Ala Gly Thr Leu Gly Asp Tyr Trp Gly Gln 
            100                 105                 110         


Gly Thr Gln Val Thr Val Lys Pro 
        115                 120 


<210>  12
<211>  120
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  czPD-1-14v4 VHH

<400>  12

Glu Val Gln Leu Val Glu Ser Gly Gly Gly Glu Val Lys Pro Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Val Ala Ser Gly Arg Thr Val Ser Ile Tyr 
            20                  25                  30          


Ala Met Gly Trp Phe Arg Gln Ala Pro Gly Lys Glu Arg Glu Phe Val 
        35                  40                  45              


Ala Gly Ile Gly Trp Asn Gly Gly Thr Thr Tyr Tyr Ala Asp Ala Val 
    50                  55                  60                  


Lys Gly Arg Phe Thr Ile Ser Arg Asp His Ala Lys Asn Thr Ala Tyr 
65                  70                  75                  80  


Leu Gln Met Ser Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Ala Gln Glu Ser Ala Ala Gly Thr Leu Gly Asp Tyr Trp Gly Gln 
            100                 105                 110         


Gly Thr Gln Val Thr Val Lys Pro 
        115                 120 


<210>  13
<211>  120
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  czPD-1-14v5 VHH

<400>  13

Glu Val Gln Leu Val Glu Ser Gly Gly Gly Glu Val Lys Pro Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Arg Thr Val Ser Ile Tyr 
            20                  25                  30          


Ala Met Gly Trp Phe Arg Gln Ala Pro Gly Lys Glu Arg Glu Phe Val 
        35                  40                  45              


Ala Gly Ile Gly Trp Asn Gly Gly Thr Thr Tyr Tyr Ala Asp Ala Val 
    50                  55                  60                  


Glu Gly Arg Phe Thr Ile Ser Arg Asp His Ala Lys Asn Thr Ala Tyr 
65                  70                  75                  80  


Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Ala Gln Glu Ser Ala Ala Gly Thr Leu Gly Asp Tyr Trp Gly Gln 
            100                 105                 110         


Gly Thr Gln Val Thr Val Lys Pro 
        115                 120 


<210>  14
<211>  355
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  czPD1-14v1-Fc xEML-DANA

<400>  14

Glu Val Gln Leu Val Glu Ser Gly Gly Gly Glu Val Lys Pro Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Val Ala Ser Gly Arg Thr Val Ser Ile Tyr 
            20                  25                  30          


Ala Met Gly Trp Phe Arg Gln Ala Pro Gly Lys Glu Arg Glu Phe Val 
        35                  40                  45              


Ala Gly Ile Gly Trp Ser Gly Gly Thr Thr Tyr Tyr Ala Asp Ala Val 
    50                  55                  60                  


Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys Asn Thr Val Tyr 
65                  70                  75                  80  


Leu Gln Met Ser Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Ala Gln Glu Ser Ala Ala Gly Thr Leu Gly Asp Tyr Trp Gly Gln 
            100                 105                 110         


Gly Thr Gln Val Thr Val Lys Pro Gly Gly Gly Gly Glu Asn Gly Arg 
        115                 120                 125             


Val Pro Arg Pro Pro Asp Cys Pro Lys Cys Pro Ala Pro Gly Gly Pro 
    130                 135                 140                 


Ser Val Phe Ile Phe Pro Pro Lys Pro Lys Asp Thr Leu Leu Ile Ala 
145                 150                 155                 160 


Arg Thr Pro Glu Val Thr Cys Val Val Val Ala Leu Asp Pro Glu Asp 
                165                 170                 175     


Pro Glu Val Gln Ile Ser Trp Phe Val Asp Gly Lys Gln Met Gln Thr 
            180                 185                 190         


Ala Lys Thr Gln Pro Arg Glu Glu Gln Phe Ala Gly Thr Tyr Arg Val 
        195                 200                 205             


Val Ser Val Leu Pro Ile Gly His Gln Asp Trp Leu Lys Gly Lys Gln 
    210                 215                 220                 


Phe Thr Cys Lys Val Asn Asn Lys Ala Leu Pro Ser Pro Ile Glu Arg 
225                 230                 235                 240 


Thr Ile Ser Lys Ala Arg Gly Gln Ala His Gln Pro Ser Val Tyr Val 
                245                 250                 255     


Leu Pro Pro Ser Arg Glu Glu Leu Ser Lys Asn Thr Val Ser Leu Thr 
            260                 265                 270         


Cys Leu Ile Lys Asp Phe Phe Pro Pro Asp Ile Asp Val Glu Trp Gln 
        275                 280                 285             


Ser Asn Gly Gln Gln Glu Pro Glu Ser Lys Tyr Arg Thr Thr Pro Pro 
    290                 295                 300                 


Gln Leu Asp Glu Asp Gly Ser Tyr Phe Leu Tyr Ser Lys Leu Ser Val 
305                 310                 315                 320 


Asp Lys Ser Arg Trp Gln Arg Gly Asp Thr Phe Ile Cys Ala Val Met 
                325                 330                 335     


His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser His Ser 
            340                 345                 350         


Pro Gly Lys 
        355 


<210>  15
<211>  355
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  czPD1-14v2-Fc xEML-DANA

<400>  15

Glu Val Gln Leu Val Glu Ser Gly Gly Gly Glu Val Lys Pro Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Val Ala Ser Gly Arg Thr Val Ser Ile Tyr 
            20                  25                  30          


Ala Met Gly Trp Phe Arg Gln Ala Pro Gly Lys Glu Arg Glu Phe Val 
        35                  40                  45              


Ala Gly Ile Gly Trp Asn Gly Gly Thr Thr Tyr Tyr Ala Asp Ala Val 
    50                  55                  60                  


Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys Asn Thr Val Tyr 
65                  70                  75                  80  


Leu Gln Met Ser Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Ala Gln Glu Ser Ala Ala Gly Thr Leu Gly Asp Tyr Trp Gly Gln 
            100                 105                 110         


Gly Thr Gln Val Thr Val Lys Pro Gly Gly Gly Gly Glu Asn Gly Arg 
        115                 120                 125             


Val Pro Arg Pro Pro Asp Cys Pro Lys Cys Pro Ala Pro Gly Gly Pro 
    130                 135                 140                 


Ser Val Phe Ile Phe Pro Pro Lys Pro Lys Asp Thr Leu Leu Ile Ala 
145                 150                 155                 160 


Arg Thr Pro Glu Val Thr Cys Val Val Val Ala Leu Asp Pro Glu Asp 
                165                 170                 175     


Pro Glu Val Gln Ile Ser Trp Phe Val Asp Gly Lys Gln Met Gln Thr 
            180                 185                 190         


Ala Lys Thr Gln Pro Arg Glu Glu Gln Phe Ala Gly Thr Tyr Arg Val 
        195                 200                 205             


Val Ser Val Leu Pro Ile Gly His Gln Asp Trp Leu Lys Gly Lys Gln 
    210                 215                 220                 


Phe Thr Cys Lys Val Asn Asn Lys Ala Leu Pro Ser Pro Ile Glu Arg 
225                 230                 235                 240 


Thr Ile Ser Lys Ala Arg Gly Gln Ala His Gln Pro Ser Val Tyr Val 
                245                 250                 255     


Leu Pro Pro Ser Arg Glu Glu Leu Ser Lys Asn Thr Val Ser Leu Thr 
            260                 265                 270         


Cys Leu Ile Lys Asp Phe Phe Pro Pro Asp Ile Asp Val Glu Trp Gln 
        275                 280                 285             


Ser Asn Gly Gln Gln Glu Pro Glu Ser Lys Tyr Arg Thr Thr Pro Pro 
    290                 295                 300                 


Gln Leu Asp Glu Asp Gly Ser Tyr Phe Leu Tyr Ser Lys Leu Ser Val 
305                 310                 315                 320 


Asp Lys Ser Arg Trp Gln Arg Gly Asp Thr Phe Ile Cys Ala Val Met 
                325                 330                 335     


His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser His Ser 
            340                 345                 350         


Pro Gly Lys 
        355 


<210>  16
<211>  355
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  czPD-1-14v3-Fc xEML-DANA

<400>  16

Glu Val Gln Leu Val Glu Ser Gly Gly Gly Glu Val Lys Pro Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Val Ala Ser Gly Arg Thr Val Ser Ile Tyr 
            20                  25                  30          


Ala Met Gly Trp Phe Arg Gln Ala Pro Gly Lys Glu Arg Glu Phe Val 
        35                  40                  45              


Ala Gly Ile Gly Trp Asn Gly Gly Thr Thr Tyr Tyr Ala Asp Ala Val 
    50                  55                  60                  


Lys Gly Arg Phe Thr Ile Ser Arg Asp His Ala Lys Asn Thr Val Tyr 
65                  70                  75                  80  


Leu Gln Met Ser Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Ala Gln Glu Ser Ala Ala Gly Thr Leu Gly Asp Tyr Trp Gly Gln 
            100                 105                 110         


Gly Thr Gln Val Thr Val Lys Pro Gly Gly Gly Gly Glu Asn Gly Arg 
        115                 120                 125             


Val Pro Arg Pro Pro Asp Cys Pro Lys Cys Pro Ala Pro Gly Gly Pro 
    130                 135                 140                 


Ser Val Phe Ile Phe Pro Pro Lys Pro Lys Asp Thr Leu Leu Ile Ala 
145                 150                 155                 160 


Arg Thr Pro Glu Val Thr Cys Val Val Val Ala Leu Asp Pro Glu Asp 
                165                 170                 175     


Pro Glu Val Gln Ile Ser Trp Phe Val Asp Gly Lys Gln Met Gln Thr 
            180                 185                 190         


Ala Lys Thr Gln Pro Arg Glu Glu Gln Phe Ala Gly Thr Tyr Arg Val 
        195                 200                 205             


Val Ser Val Leu Pro Ile Gly His Gln Asp Trp Leu Lys Gly Lys Gln 
    210                 215                 220                 


Phe Thr Cys Lys Val Asn Asn Lys Ala Leu Pro Ser Pro Ile Glu Arg 
225                 230                 235                 240 


Thr Ile Ser Lys Ala Arg Gly Gln Ala His Gln Pro Ser Val Tyr Val 
                245                 250                 255     


Leu Pro Pro Ser Arg Glu Glu Leu Ser Lys Asn Thr Val Ser Leu Thr 
            260                 265                 270         


Cys Leu Ile Lys Asp Phe Phe Pro Pro Asp Ile Asp Val Glu Trp Gln 
        275                 280                 285             


Ser Asn Gly Gln Gln Glu Pro Glu Ser Lys Tyr Arg Thr Thr Pro Pro 
    290                 295                 300                 


Gln Leu Asp Glu Asp Gly Ser Tyr Phe Leu Tyr Ser Lys Leu Ser Val 
305                 310                 315                 320 


Asp Lys Ser Arg Trp Gln Arg Gly Asp Thr Phe Ile Cys Ala Val Met 
                325                 330                 335     


His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser His Ser 
            340                 345                 350         


Pro Gly Lys 
        355 


<210>  17
<211>  355
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  czPD-1-14v4-Fc xEML-DANA

<400>  17

Glu Val Gln Leu Val Glu Ser Gly Gly Gly Glu Val Lys Pro Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Val Ala Ser Gly Arg Thr Val Ser Ile Tyr 
            20                  25                  30          


Ala Met Gly Trp Phe Arg Gln Ala Pro Gly Lys Glu Arg Glu Phe Val 
        35                  40                  45              


Ala Gly Ile Gly Trp Asn Gly Gly Thr Thr Tyr Tyr Ala Asp Ala Val 
    50                  55                  60                  


Lys Gly Arg Phe Thr Ile Ser Arg Asp His Ala Lys Asn Thr Ala Tyr 
65                  70                  75                  80  


Leu Gln Met Ser Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Ala Gln Glu Ser Ala Ala Gly Thr Leu Gly Asp Tyr Trp Gly Gln 
            100                 105                 110         


Gly Thr Gln Val Thr Val Lys Pro Gly Gly Gly Gly Glu Asn Gly Arg 
        115                 120                 125             


Val Pro Arg Pro Pro Asp Cys Pro Lys Cys Pro Ala Pro Gly Gly Pro 
    130                 135                 140                 


Ser Val Phe Ile Phe Pro Pro Lys Pro Lys Asp Thr Leu Leu Ile Ala 
145                 150                 155                 160 


Arg Thr Pro Glu Val Thr Cys Val Val Val Ala Leu Asp Pro Glu Asp 
                165                 170                 175     


Pro Glu Val Gln Ile Ser Trp Phe Val Asp Gly Lys Gln Met Gln Thr 
            180                 185                 190         


Ala Lys Thr Gln Pro Arg Glu Glu Gln Phe Ala Gly Thr Tyr Arg Val 
        195                 200                 205             


Val Ser Val Leu Pro Ile Gly His Gln Asp Trp Leu Lys Gly Lys Gln 
    210                 215                 220                 


Phe Thr Cys Lys Val Asn Asn Lys Ala Leu Pro Ser Pro Ile Glu Arg 
225                 230                 235                 240 


Thr Ile Ser Lys Ala Arg Gly Gln Ala His Gln Pro Ser Val Tyr Val 
                245                 250                 255     


Leu Pro Pro Ser Arg Glu Glu Leu Ser Lys Asn Thr Val Ser Leu Thr 
            260                 265                 270         


Cys Leu Ile Lys Asp Phe Phe Pro Pro Asp Ile Asp Val Glu Trp Gln 
        275                 280                 285             


Ser Asn Gly Gln Gln Glu Pro Glu Ser Lys Tyr Arg Thr Thr Pro Pro 
    290                 295                 300                 


Gln Leu Asp Glu Asp Gly Ser Tyr Phe Leu Tyr Ser Lys Leu Ser Val 
305                 310                 315                 320 


Asp Lys Ser Arg Trp Gln Arg Gly Asp Thr Phe Ile Cys Ala Val Met 
                325                 330                 335     


His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser His Ser 
            340                 345                 350         


Pro Gly Lys 
        355 


<210>  18
<211>  355
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  czPD-1-14v5-Fc xEML-DANA

<400>  18

Glu Val Gln Leu Val Glu Ser Gly Gly Gly Glu Val Lys Pro Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Arg Thr Val Ser Ile Tyr 
            20                  25                  30          


Ala Met Gly Trp Phe Arg Gln Ala Pro Gly Lys Glu Arg Glu Phe Val 
        35                  40                  45              


Ala Gly Ile Gly Trp Asn Gly Gly Thr Thr Tyr Tyr Ala Asp Ala Val 
    50                  55                  60                  


Glu Gly Arg Phe Thr Ile Ser Arg Asp His Ala Lys Asn Thr Ala Tyr 
65                  70                  75                  80  


Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Ala Gln Glu Ser Ala Ala Gly Thr Leu Gly Asp Tyr Trp Gly Gln 
            100                 105                 110         


Gly Thr Gln Val Thr Val Lys Pro Gly Gly Gly Gly Glu Asn Gly Arg 
        115                 120                 125             


Val Pro Arg Pro Pro Asp Cys Pro Lys Cys Pro Ala Pro Gly Gly Pro 
    130                 135                 140                 


Ser Val Phe Ile Phe Pro Pro Lys Pro Lys Asp Thr Leu Leu Ile Ala 
145                 150                 155                 160 


Arg Thr Pro Glu Val Thr Cys Val Val Val Ala Leu Asp Pro Glu Asp 
                165                 170                 175     


Pro Glu Val Gln Ile Ser Trp Phe Val Asp Gly Lys Gln Met Gln Thr 
            180                 185                 190         


Ala Lys Thr Gln Pro Arg Glu Glu Gln Phe Ala Gly Thr Tyr Arg Val 
        195                 200                 205             


Val Ser Val Leu Pro Ile Gly His Gln Asp Trp Leu Lys Gly Lys Gln 
    210                 215                 220                 


Phe Thr Cys Lys Val Asn Asn Lys Ala Leu Pro Ser Pro Ile Glu Arg 
225                 230                 235                 240 


Thr Ile Ser Lys Ala Arg Gly Gln Ala His Gln Pro Ser Val Tyr Val 
                245                 250                 255     


Leu Pro Pro Ser Arg Glu Glu Leu Ser Lys Asn Thr Val Ser Leu Thr 
            260                 265                 270         


Cys Leu Ile Lys Asp Phe Phe Pro Pro Asp Ile Asp Val Glu Trp Gln 
        275                 280                 285             


Ser Asn Gly Gln Gln Glu Pro Glu Ser Lys Tyr Arg Thr Thr Pro Pro 
    290                 295                 300                 


Gln Leu Asp Glu Asp Gly Ser Tyr Phe Leu Tyr Ser Lys Leu Ser Val 
305                 310                 315                 320 


Asp Lys Ser Arg Trp Gln Arg Gly Asp Thr Phe Ile Cys Ala Val Met 
                325                 330                 335     


His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser His Ser 
            340                 345                 350         


Pro Gly Lys 
        355 


<210>  19
<211>  234
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Canine IgGB Fc region

<400>  19

Glu Asn Gly Arg Val Pro Arg Pro Pro Asp Cys Pro Pro Cys Pro Ala 
1               5                   10                  15      


Pro Glu Met Leu Gly Gly Pro Ser Val Phe Ile Phe Pro Pro Lys Pro 
            20                  25                  30          


Lys Asp Thr Leu Leu Ile Ala Arg Thr Pro Glu Val Thr Cys Val Val 
        35                  40                  45              


Val Asp Leu Asp Pro Glu Asp Pro Glu Val Gln Ile Ser Trp Phe Val 
    50                  55                  60                  


Asp Gly Lys Gln Met Gln Thr Ala Lys Thr Gln Pro Arg Glu Glu Gln 
65                  70                  75                  80  


Phe Asn Gly Thr Tyr Arg Val Val Ser Val Leu Pro Ile Gly His Gln 
                85                  90                  95      


Asp Trp Leu Lys Gly Lys Gln Phe Thr Cys Lys Val Asn Asn Lys Ala 
            100                 105                 110         


Leu Pro Ser Pro Ile Glu Arg Thr Ile Ser Lys Ala Arg Gly Gln Ala 
        115                 120                 125             


His Gln Pro Ser Val Tyr Val Leu Pro Pro Ser Arg Glu Glu Leu Ser 
    130                 135                 140                 


Lys Asn Thr Val Ser Leu Thr Cys Leu Ile Lys Asp Phe Phe Pro Pro 
145                 150                 155                 160 


Asp Ile Asp Val Glu Trp Gln Ser Asn Gly Gln Gln Glu Pro Glu Ser 
                165                 170                 175     


Lys Tyr Arg Thr Thr Pro Pro Gln Leu Asp Glu Asp Gly Ser Tyr Phe 
            180                 185                 190         


Leu Tyr Ser Lys Leu Ser Val Asp Lys Ser Arg Trp Gln Arg Gly Asp 
        195                 200                 205             


Thr Phe Ile Cys Ala Val Met His Glu Ala Leu His Asn His Tyr Thr 
    210                 215                 220                 


Gln Lys Ser Leu Ser His Ser Pro Gly Lys 
225                 230                 


<210>  20
<211>  231
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Canine IgGB Fc region (xEML-DANA)

<400>  20

Glu Asn Gly Arg Val Pro Arg Pro Pro Asp Cys Pro Lys Cys Pro Ala 
1               5                   10                  15      


Pro Gly Gly Pro Ser Val Phe Ile Phe Pro Pro Lys Pro Lys Asp Thr 
            20                  25                  30          


Leu Leu Ile Ala Arg Thr Pro Glu Val Thr Cys Val Val Val Ala Leu 
        35                  40                  45              


Asp Pro Glu Asp Pro Glu Val Gln Ile Ser Trp Phe Val Asp Gly Lys 
    50                  55                  60                  


Gln Met Gln Thr Ala Lys Thr Gln Pro Arg Glu Glu Gln Phe Ala Gly 
65                  70                  75                  80  


Thr Tyr Arg Val Val Ser Val Leu Pro Ile Gly His Gln Asp Trp Leu 
                85                  90                  95      


Lys Gly Lys Gln Phe Thr Cys Lys Val Asn Asn Lys Ala Leu Pro Ser 
            100                 105                 110         


Pro Ile Glu Arg Thr Ile Ser Lys Ala Arg Gly Gln Ala His Gln Pro 
        115                 120                 125             


Ser Val Tyr Val Leu Pro Pro Ser Arg Glu Glu Leu Ser Lys Asn Thr 
    130                 135                 140                 


Val Ser Leu Thr Cys Leu Ile Lys Asp Phe Phe Pro Pro Asp Ile Asp 
145                 150                 155                 160 


Val Glu Trp Gln Ser Asn Gly Gln Gln Glu Pro Glu Ser Lys Tyr Arg 
                165                 170                 175     


Thr Thr Pro Pro Gln Leu Asp Glu Asp Gly Ser Tyr Phe Leu Tyr Ser 
            180                 185                 190         


Lys Leu Ser Val Asp Lys Ser Arg Trp Gln Arg Gly Asp Thr Phe Ile 
        195                 200                 205             


Cys Ala Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser 
    210                 215                 220                 


Leu Ser His Ser Pro Gly Lys 
225                 230     


<210>  21
<211>  355
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  PD1-14-Fc xEML-DANA

<400>  21

Gln Val Gln Leu Gln Gln Ser Gly Gly Gly Leu Ala Gln Ala Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Arg Thr Val Ser Ile Tyr 
            20                  25                  30          


Ala Met Gly Trp Phe Arg Gln Ala Pro Gly Lys Glu Arg Glu Phe Val 
        35                  40                  45              


Ala Gly Ile Gly Trp Asn Gly Gly Thr Thr Tyr Tyr Ala Asp Ser Val 
    50                  55                  60                  


Glu Gly Arg Phe Thr Ile Ser Arg Asp His Ala Lys Asn Thr Ala Tyr 
65                  70                  75                  80  


Leu Gln Met Asn Ser Leu Lys Pro Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Ala Gln Glu Ser Ala Ala Gly Thr Leu Gly Asp Tyr Trp Gly Arg 
            100                 105                 110         


Gly Thr Gln Val Thr Val Lys Pro Gly Gly Gly Gly Glu Asn Gly Arg 
        115                 120                 125             


Val Pro Arg Pro Pro Asp Cys Pro Lys Cys Pro Ala Pro Gly Gly Pro 
    130                 135                 140                 


Ser Val Phe Ile Phe Pro Pro Lys Pro Lys Asp Thr Leu Leu Ile Ala 
145                 150                 155                 160 


Arg Thr Pro Glu Val Thr Cys Val Val Val Ala Leu Asp Pro Glu Asp 
                165                 170                 175     


Pro Glu Val Gln Ile Ser Trp Phe Val Asp Gly Lys Gln Met Gln Thr 
            180                 185                 190         


Ala Lys Thr Gln Pro Arg Glu Glu Gln Phe Ala Gly Thr Tyr Arg Val 
        195                 200                 205             


Val Ser Val Leu Pro Ile Gly His Gln Asp Trp Leu Lys Gly Lys Gln 
    210                 215                 220                 


Phe Thr Cys Lys Val Asn Asn Lys Ala Leu Pro Ser Pro Ile Glu Arg 
225                 230                 235                 240 


Thr Ile Ser Lys Ala Arg Gly Gln Ala His Gln Pro Ser Val Tyr Val 
                245                 250                 255     


Leu Pro Pro Ser Arg Glu Glu Leu Ser Lys Asn Thr Val Ser Leu Thr 
            260                 265                 270         


Cys Leu Ile Lys Asp Phe Phe Pro Pro Asp Ile Asp Val Glu Trp Gln 
        275                 280                 285             


Ser Asn Gly Gln Gln Glu Pro Glu Ser Lys Tyr Arg Thr Thr Pro Pro 
    290                 295                 300                 


Gln Leu Asp Glu Asp Gly Ser Tyr Phe Leu Tyr Ser Lys Leu Ser Val 
305                 310                 315                 320 


Asp Lys Ser Arg Trp Gln Arg Gly Asp Thr Phe Ile Cys Ala Val Met 
                325                 330                 335     


His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser His Ser 
            340                 345                 350         


Pro Gly Lys 
        355 


<210>  22
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  PD1-8 CDR1

<400>  22

Gly Arg Thr Phe Ser Ser Tyr Gly Met Gly 
1               5                   10  


<210>  23
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  PD1-8 CDR2

<400>  23

Ala Ile Ser Trp Ser Gly Gly Thr Gln Tyr 
1               5                   10  


<210>  24
<211>  14
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  PD1-8 CDR3

<400>  24

Tyr Thr Asp Tyr Val Val Tyr Arg Pro Gln Glu Ile Gly Tyr 
1               5                   10                  


<210>  25
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  PD1-13 CDR1

<400>  25

Gly Arg Thr Glu Ile Ile Tyr Ala Met Gly 
1               5                   10  


<210>  26
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  PD1-13 CDR2

<400>  26

Gly Ile Gly Trp Ser Gly Gly Thr Thr Tyr 
1               5                   10  


<210>  27
<211>  11
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  PD1-13 CDR3

<400>  27

Gln Asp Ser Val Ala Gly Thr Leu Gly Asp Tyr 
1               5                   10      


<210>  28
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  PD1-5 CDR1

<400>  28

Gly Leu Thr Phe Gly Leu Tyr Ala Met Thr 
1               5                   10  


<210>  29
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  PD1-5 CDR2

<400>  29

Gly Ile Gly Trp Ser Gly Gly Thr Thr Tyr 
1               5                   10  


<210>  30
<211>  19
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  PD1-5 CDR3

<400>  30

Thr Asp Tyr Glu Thr Arg Cys Asp Tyr Gly Leu Arg Leu Arg Asp Arg 
1               5                   10                  15      


Thr Ala Tyr 
            


<210>  31
<211>  355
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  PD1-13-Fc xEML-DANA

<400>  31

Gln Val Thr Leu Lys Glu Ser Gly Gly Gly Leu Val Gln Ala Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Arg Thr Glu Ile Ile Tyr 
            20                  25                  30          


Ala Met Gly Trp Phe Arg Gln Ala Pro Gly Lys Glu Arg Glu Phe Val 
        35                  40                  45              


Ala Gly Ile Gly Trp Ser Gly Gly Thr Thr Tyr Tyr Ala Asp Ser Val 
    50                  55                  60                  


Glu Gly Arg Phe Thr Ile Ser Arg Asp Ser Ala Lys Asn Thr Val Tyr 
65                  70                  75                  80  


Leu Gln Met Arg Ser Leu Lys Pro Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Ala Gln Asp Ser Val Ala Gly Thr Leu Gly Asp Tyr Trp Gly Gln 
            100                 105                 110         


Gly Thr Gln Val Thr Val Lys Pro Gly Gly Gly Gly Glu Asn Gly Arg 
        115                 120                 125             


Val Pro Arg Pro Pro Asp Cys Pro Lys Cys Pro Ala Pro Gly Gly Pro 
    130                 135                 140                 


Ser Val Phe Ile Phe Pro Pro Lys Pro Lys Asp Thr Leu Leu Ile Ala 
145                 150                 155                 160 


Arg Thr Pro Glu Val Thr Cys Val Val Val Ala Leu Asp Pro Glu Asp 
                165                 170                 175     


Pro Glu Val Gln Ile Ser Trp Phe Val Asp Gly Lys Gln Met Gln Thr 
            180                 185                 190         


Ala Lys Thr Gln Pro Arg Glu Glu Gln Phe Ala Gly Thr Tyr Arg Val 
        195                 200                 205             


Val Ser Val Leu Pro Ile Gly His Gln Asp Trp Leu Lys Gly Lys Gln 
    210                 215                 220                 


Phe Thr Cys Lys Val Asn Asn Lys Ala Leu Pro Ser Pro Ile Glu Arg 
225                 230                 235                 240 


Thr Ile Ser Lys Ala Arg Gly Gln Ala His Gln Pro Ser Val Tyr Val 
                245                 250                 255     


Leu Pro Pro Ser Arg Glu Glu Leu Ser Lys Asn Thr Val Ser Leu Thr 
            260                 265                 270         


Cys Leu Ile Lys Asp Phe Phe Pro Pro Asp Ile Asp Val Glu Trp Gln 
        275                 280                 285             


Ser Asn Gly Gln Gln Glu Pro Glu Ser Lys Tyr Arg Thr Thr Pro Pro 
    290                 295                 300                 


Gln Leu Asp Glu Asp Gly Ser Tyr Phe Leu Tyr Ser Lys Leu Ser Val 
305                 310                 315                 320 


Asp Lys Ser Arg Trp Gln Arg Gly Asp Thr Phe Ile Cys Ala Val Met 
                325                 330                 335     


His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser His Ser 
            340                 345                 350         


Pro Gly Lys 
        355 


