                         SEQUENCE LISTING

<110>  POLICLINICO SAN DONATO S.p.A. -
 
<120>  Proline hydroxylase inhibitors for use in the skeletal muscle 
       regeneration

<130>  P1850PC

<150>  EP20173531.3
<151>  2020-05-07

<160>  48    

<170>  PatentIn version 3.5

<210>  1
<211>  28
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer forward

<400>  1
ggggtacccc ggaagctatc acaggctt                                          28


<210>  2
<211>  30
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Reverse primer

<400>  2
gggttcgaac cctagcatcc ttcgctaact                                        30


<210>  3
<211>  17
<212>  DNA
<213>  Artificial sequence

<220>
<223>  WNT7a1 forward primer

<400>  3
ggaagctatc acaggct                                                      17


<210>  4
<211>  16
<212>  DNA
<213>  Artificial sequence

<220>
<223>  WNT7a1 reverse primer

<400>  4
accgtggatt caagga                                                       16


<210>  5
<211>  18
<212>  DNA
<213>  Artificial sequence

<220>
<223>  WNT7a2 forward primer

<400>  5
tgtcagcatg gctggcgc                                                     18


<210>  6
<211>  18
<212>  DNA
<213>  Artificial sequence

<220>
<223>  WNT7a2 reverse primer

<400>  6
tgtcagcatg gctggcgc                                                     18


<210>  7
<211>  19
<212>  DNA
<213>  Artificial sequence

<220>
<223>  WNT7a3 forward primer

<400>  7
gcagtctgga aggctaagc                                                    19


<210>  8
<211>  17
<212>  DNA
<213>  Artificial sequence

<220>
<223>  WNT7a3 reverse primer

<400>  8
gtcaaactca aagtcac                                                      17


<210>  9
<211>  21
<212>  DNA
<213>  Artificial sequence

<220>
<223>  WNT7a4 forward primer

<400>  9
ttcacctcct cagtttctcc a                                                 21


<210>  10
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  WNT7a4 reverse primer

<400>  10
agcccaatga ccagtagcag                                                   20


<210>  11
<211>  19
<212>  DNA
<213>  Artificial sequence

<220>
<223>  WNT7a5 forward primer

<400>  11
tccagtccaa ttccaagtc                                                    19


<210>  12
<211>  18
<212>  DNA
<213>  Artificial sequence

<220>
<223>  WNT7a5 reverse primer

<400>  12
gtctgtgcga ggggctga                                                     18


<210>  13
<211>  18
<212>  DNA
<213>  Artificial sequence

<220>
<223>  WNT7a6 forward primer

<400>  13
gagggtgagg agagaatg                                                     18


<210>  14
<211>  19
<212>  DNA
<213>  Artificial sequence

<220>
<223>  WNT7a6 reverse primer

<400>  14
ccttgttaga gctctgtca                                                    19


<210>  15
<211>  18
<212>  DNA
<213>  Artificial sequence

<220>
<223>  WNT7a7 forward primer

<400>  15
cgtgagggtt ctgaaatg                                                     18


<210>  16
<211>  18
<212>  DNA
<213>  Artificial sequence

<220>
<223>  WNT7a7 reverse primer

<400>  16
ggaatcctgc ctgcctag                                                     18


<210>  17
<211>  15
<212>  DNA
<213>  Artificial sequence

<220>
<223>  VEGF forward primer

<400>  17
aaaaacgaaa gcgca                                                        15


<210>  18
<211>  15
<212>  DNA
<213>  Artificial sequence

<220>
<223>  VEGF reverse primer

<400>  18
tttctccgct ctgaa                                                        15


<210>  19
<211>  19
<212>  DNA
<213>  Artificial sequence

<220>
<223>  PHD2 forward primer

<400>  19
ctgtggaaca gcccttttt                                                    19


<210>  20
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  PHD2 reverse primer

<400>  20
cgagtctctc tgcgaatcct                                                   20


<210>  21
<211>  15
<212>  DNA
<213>  Artificial sequence

<220>
<223>  MYOG forward primer

<400>  21
agccacactg aggga                                                        15


<210>  22
<211>  15
<212>  DNA
<213>  Artificial sequence

<220>
<223>  MYOG reverse primer

<400>  22
gttgagggag ctgag                                                        15


<210>  23
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  MHC forward primer

<400>  23
tggagcagga ggaatacaag                                                   20


<210>  24
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  MHC reverse primer

<400>  24
gcatagtgga tgagggagaa                                                   20


<210>  25
<211>  17
<212>  DNA
<213>  Artificial sequence

<220>
<223>  WNT7a forward primer

<400>  25
actgtggctg cgacaag                                                      17


<210>  26
<211>  18
<212>  DNA
<213>  Artificial sequence

<220>
<223>  WNT7a reverse primer

<400>  26
cttcatgttc tcctccag                                                     18


<210>  27
<211>  18
<212>  DNA
<213>  Artificial sequence

<220>
<223>  MYOR forward primer

<400>  27
gcccagcgac atttcttc                                                     18


<210>  28
<211>  18
<212>  DNA
<213>  Artificial sequence

<220>
<223>  MYOR reverse primer

<400>  28
cgcttcctct tgcatcct                                                     18


<210>  29
<211>  17
<212>  DNA
<213>  Artificial sequence

<220>
<223>  RPL13 forward primer

<400>  29
ctcggccgtt cctgtat                                                      17


<210>  30
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  RPL13 reverse primer

<400>  30
gtggaagtgg ggcttcagta                                                   20


<210>  31
<211>  24
<212>  DNA
<213>  Artificial sequence

<220>
<223>  WNT7a target sequence

<400>  31
gggcatagtc tacctccgga tcgg                                              24


<210>  32
<211>  20
<212>  RNA
<213>  Artificial sequence

<220>
<223>  Negative control sequence

<400>  32
aaaugugaga ucagaguaau                                                   20


<210>  33
<211>  19
<212>  DNA
<213>  Artificial sequence

<220>
<223>  WNT7a forward primer

<400>  33
cttgttgcgc ttgttctcc                                                    19


<210>  34
<211>  18
<212>  DNA
<213>  Artificial sequence

<220>
<223>  WNT7a reverse primer

<400>  34
cgcaattcca cagactcg                                                     18


<210>  35
<211>  17
<212>  DNA
<213>  Artificial sequence

<220>
<223>  RPLI forward primer

<400>  35
ctcggccgtt cctgtat                                                      17


<210>  36
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  RPLI reverse primer

<400>  36
gtggaagtgg ggcttcagta                                                   20


<210>  37
<211>  19
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Wild type HRE binding sequence  of WNT7a promoter

<400>  37
gtctcccgtg ttccttgaa                                                    19


<210>  38
<211>  15
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Deletion 1.1. of HRE sequence in the WNT7a promoter

<400>  38
gtctccttcc ttgaa                                                        15


<210>  39
<211>  17
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Wild type HRE sequence of WNT7a promoter

<400>  39
tgtcgccgtg agagcgc                                                      17


<210>  40
<211>  13
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Deletion 4.2 of the HRE binding sequence of WNT7a promoter

<400>  40
tgtcgcagag cgc                                                          13


<210>  41
<211>  16
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Wild type of the HRE binding sequence of the WNT7a promoter

<400>  41
ttgggctgcg tgtctc                                                       16


<210>  42
<211>  12
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Deletion 4.1 of the HRE binding sequence of the WNT7a promoter

<400>  42
ttgggctgtc tc                                                           12


<210>  43
<211>  17
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Wild type HRE binding sequence of WNT7a pomoter

<400>  43
cctgcgcgtg ttctcag                                                      17


<210>  44
<211>  13
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Deletion 4.3 of the HRE binding sequence of the WNT7a promoter

<400>  44
cctgcgttct cag                                                          13


<210>  45
<211>  50
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Wild type sequence WNT7a promoter

<400>  45
gggcatagtc tacctccgga tcgggtaagc accgcgcgcg ttctttccgc                  50


<210>  46
<211>  50
<212>  DNA
<213>  Artificial sequence

<220>
<223>  KO of WNT7a promoter

<400>  46
gggcttaccc tacctccgga tggggaaggc cccgggcggt tcctttccgc                  50


<210>  47
<211>  21
<212>  DNA
<213>  Artificial sequence

<220>
<223>  PHD3 forward primer

<400>  47
ctatgtcaag gagcggtcca a                                                 21


<210>  48
<211>  21
<212>  DNA
<213>  Artificial sequence

<220>
<223>  PHD3 reverse primer

<400>  48
gtccacatgg cgaacataac c                                                 21


