                         SEQUENCE LISTING

<110>  InflaRx GmbH
 
<120>  Humanized anti-C5a antibodies

<130>  680-10 PCT

<150>  EP20173255.9
<151>  2020-05-06

<160>  25    

<170>  PatentIn version 3.5

<210>  1
<211>  74
<212>  PRT
<213>  Homo sapiens

<400>  1

Thr Leu Gln Lys Lys Ile Glu Glu Ile Ala Ala Lys Tyr Lys His Ser 
1               5                   10                  15      


Val Val Lys Lys Cys Cys Tyr Asp Gly Ala Cys Val Asn Asn Asp Glu 
            20                  25                  30          


Thr Cys Glu Gln Arg Ala Ala Arg Ile Ser Leu Gly Pro Arg Cys Ile 
        35                  40                  45              


Lys Ala Phe Thr Glu Cys Cys Val Val Ala Ser Gln Leu Arg Ala Asn 
    50                  55                  60                  


Ile Ser His Lys Asp Met Gln Leu Gly Arg 
65                  70                  


<210>  2
<211>  9
<212>  PRT
<213>  Homo sapiens

<400>  2

Asn Asp Glu Thr Cys Glu Gln Arg Ala 
1               5                   


<210>  3
<211>  7
<212>  PRT
<213>  Homo sapiens

<400>  3

Ser His Lys Asp Met Gln Leu 
1               5           


<210>  4
<211>  7
<212>  PRT
<213>  Homo sapiens

<400>  4

Asp Glu Thr Cys Glu Gln Arg 
1               5           


<210>  5
<211>  5
<212>  PRT
<213>  Homo sapiens

<400>  5

His Lys Asp Met Gln 
1               5   


<210>  6
<211>  119
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VH0

<400>  6

Gln Val Gln Leu Gln Gln Ser Gly Pro Gln Leu Val Arg Pro Gly Thr 
1               5                   10                  15      


Ser Val Lys Ile Ser Cys Lys Ala Ser Gly Tyr Ser Phe Thr Thr Phe 
            20                  25                  30          


Trp Met Asp Trp Val Lys Gln Arg Pro Gly Gln Gly Leu Glu Trp Ile 
        35                  40                  45              


Gly Arg Ile Asp Pro Ser Asp Ser Glu Ser Arg Leu Asp Gln Arg Phe 
    50                  55                  60                  


Lys Asp Arg Ala Thr Leu Thr Val Asp Lys Ser Ser Ser Thr Val Tyr 
65                  70                  75                  80  


Met Gln Leu Ser Ser Pro Thr Ser Glu Asp Ser Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Gly Asn Asp Gly Tyr Tyr Gly Phe Ala Tyr Trp Gly Gln Gly 
            100                 105                 110         


Thr Leu Val Thr Val Ser Ser 
        115                 


<210>  7
<211>  119
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VH1

<400>  7

Gln Val Gln Leu Val Gln Ser Gly Pro Glu Leu Lys Lys Pro Gly Ala 
1               5                   10                  15      


Ser Val Lys Ile Ser Cys Lys Ala Ser Gly Tyr Ser Phe Thr Thr Phe 
            20                  25                  30          


Trp Met Asp Trp Val Lys Gln Ala Pro Gly Gln Gly Leu Glu Trp Ile 
        35                  40                  45              


Gly Arg Ile Asp Pro Ser Asp Ser Glu Ser Arg Leu Asp Gln Arg Phe 
    50                  55                  60                  


Lys Asp Arg Ala Thr Leu Thr Val Asp Lys Ser Thr Ser Thr Val Tyr 
65                  70                  75                  80  


Met Gln Leu Ser Ser Pro Thr Ser Glu Asp Ser Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Gly Asn Asp Gly Tyr Tyr Gly Phe Ala Tyr Trp Gly Gln Gly 
            100                 105                 110         


Thr Leu Val Thr Val Ser Ser 
        115                 


<210>  8
<211>  119
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VH2

<400>  8

Gln Val Gln Leu Val Gln Ser Gly Ala Glu Val Lys Lys Pro Gly Ala 
1               5                   10                  15      


Ser Val Lys Ile Ser Cys Lys Ala Ser Gly Tyr Ser Phe Thr Thr Phe 
            20                  25                  30          


Trp Met Asp Trp Val Lys Gln Ala Pro Gly Gln Gly Leu Glu Trp Ile 
        35                  40                  45              


Gly Arg Ile Asp Pro Ser Asp Ser Glu Ser Arg Leu Asp Gln Arg Phe 
    50                  55                  60                  


Lys Asp Arg Ala Thr Leu Thr Val Asp Lys Ser Thr Ser Thr Val Tyr 
65                  70                  75                  80  


Met Glu Leu Ser Ser Leu Arg Ser Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Gly Asn Asp Gly Tyr Tyr Gly Phe Ala Tyr Trp Gly Gln Gly 
            100                 105                 110         


Thr Leu Val Thr Val Ser Ser 
        115                 


<210>  9
<211>  119
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VH3

<400>  9

Gln Val Gln Leu Val Gln Ser Gly Ala Glu Val Lys Lys Pro Gly Ala 
1               5                   10                  15      


Ser Val Lys Val Ser Cys Lys Ala Ser Gly Tyr Ser Phe Thr Thr Phe 
            20                  25                  30          


Trp Met Asp Trp Val Arg Gln Ala Pro Gly Gln Gly Leu Glu Trp Ile 
        35                  40                  45              


Gly Arg Ile Asp Pro Ser Asp Ser Glu Ser Arg Leu Asp Gln Arg Phe 
    50                  55                  60                  


Lys Asp Arg Ala Thr Leu Thr Val Asp Lys Ser Thr Ser Thr Val Tyr 
65                  70                  75                  80  


Met Glu Leu Ser Ser Leu Arg Ser Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Gly Asn Asp Gly Tyr Tyr Gly Phe Ala Tyr Trp Gly Gln Gly 
            100                 105                 110         


Thr Leu Val Thr Val Ser Ser 
        115                 


<210>  10
<211>  119
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VH4

<400>  10

Gln Val Gln Leu Val Gln Ser Gly Ala Glu Val Lys Lys Pro Gly Ala 
1               5                   10                  15      


Ser Val Lys Val Ser Cys Lys Ala Ser Gly Tyr Ser Phe Thr Thr Phe 
            20                  25                  30          


Trp Met Asp Trp Val Arg Gln Ala Pro Gly Gln Gly Leu Glu Trp Ile 
        35                  40                  45              


Gly Arg Ile Asp Pro Ser Asp Ser Glu Ser Arg Leu Asp Gln Arg Phe 
    50                  55                  60                  


Lys Asp Arg Val Thr Met Thr Val Asp Lys Ser Thr Ser Thr Val Tyr 
65                  70                  75                  80  


Met Glu Leu Ser Ser Leu Arg Ser Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Gly Asn Asp Gly Tyr Tyr Gly Phe Ala Tyr Trp Gly Gln Gly 
            100                 105                 110         


Thr Leu Val Thr Val Ser Ser 
        115                 


<210>  11
<211>  119
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VH5

<400>  11

Gln Val Gln Leu Val Gln Ser Gly Ala Glu Val Lys Lys Pro Gly Ala 
1               5                   10                  15      


Ser Val Lys Val Ser Cys Lys Ala Ser Gly Tyr Ser Phe Thr Thr Phe 
            20                  25                  30          


Trp Met Asp Trp Val Arg Gln Ala Pro Gly Gln Gly Leu Glu Trp Met 
        35                  40                  45              


Gly Arg Ile Asp Pro Ser Asp Ser Glu Ser Arg Leu Asp Gln Lys Phe 
    50                  55                  60                  


Lys Asp Arg Val Thr Met Thr Val Asp Lys Ser Thr Ser Thr Val Tyr 
65                  70                  75                  80  


Met Glu Leu Ser Ser Leu Arg Ser Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Gly Asn Asp Gly Tyr Tyr Gly Phe Ala Tyr Trp Gly Gln Gly 
            100                 105                 110         


Thr Leu Val Thr Val Ser Ser 
        115                 


<210>  12
<211>  111
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  V_kappa_0

<400>  12

Asp Ile Val Leu Thr Gln Ser Pro Ala Ser Leu Ala Val Ser Leu Gly 
1               5                   10                  15      


Gln Arg Ala Thr Ile Ser Cys Lys Ala Ser Gln Ser Val Asp Tyr Asp 
            20                  25                  30          


Gly Asp Ser Tyr Met Lys Trp Tyr Gln Gln Lys Pro Gly Gln Pro Pro 
        35                  40                  45              


Lys Leu Leu Ile Tyr Ala Ala Ser Asn Leu Gln Ser Gly Ile Pro Ala 
    50                  55                  60                  


Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Asn Ile His 
65                  70                  75                  80  


Pro Val Glu Glu Glu Asp Ala Ala Thr Tyr Tyr Cys Gln Gln Ser Asn 
                85                  90                  95      


Glu Asp Pro Tyr Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile Lys 
            100                 105                 110     


<210>  13
<211>  111
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  V_kappa_1

<400>  13

Asp Ile Val Leu Thr Gln Ser Pro Ala Ser Leu Ala Ala Ser Val Gly 
1               5                   10                  15      


Asp Arg Val Thr Ile Thr Cys Lys Ala Ser Gln Ser Val Asp Tyr Asp 
            20                  25                  30          


Gly Asp Ser Tyr Met Lys Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro 
        35                  40                  45              


Lys Leu Leu Ile Tyr Ala Ala Ser Asn Leu Gln Ser Gly Ile Pro Ser 
    50                  55                  60                  


Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser 
65                  70                  75                  80  


Ser Leu Gln Glu Glu Asp Phe Ala Thr Tyr Tyr Cys Gln Gln Ser Asn 
                85                  90                  95      


Glu Asp Pro Tyr Thr Phe Gly Gln Gly Thr Lys Leu Glu Ile Lys 
            100                 105                 110     


<210>  14
<211>  111
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  V_kappa_2

<400>  14

Asp Ile Val Leu Thr Gln Ser Pro Ala Ser Leu Ser Ala Ser Val Gly 
1               5                   10                  15      


Asp Arg Val Thr Ile Thr Cys Lys Ala Ser Gln Ser Val Asp Tyr Asp 
            20                  25                  30          


Gly Asp Ser Tyr Met Lys Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro 
        35                  40                  45              


Lys Leu Leu Ile Tyr Ala Ala Ser Asn Leu Gln Ser Gly Ile Pro Ser 
    50                  55                  60                  


Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser 
65                  70                  75                  80  


Ser Leu Gln Pro Glu Asp Phe Ala Thr Tyr Tyr Cys Gln Gln Ser Asn 
                85                  90                  95      


Glu Asp Pro Tyr Thr Phe Gly Gln Gly Thr Lys Leu Glu Ile Lys 
            100                 105                 110     


<210>  15
<211>  111
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  V_kappa_3

<400>  15

Asp Ile Gln Leu Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly 
1               5                   10                  15      


Asp Arg Val Thr Ile Thr Cys Lys Ala Ser Gln Ser Val Asp Tyr Asp 
            20                  25                  30          


Gly Asp Ser Tyr Met Lys Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro 
        35                  40                  45              


Lys Leu Leu Ile Tyr Ala Ala Ser Asn Leu Gln Ser Gly Ile Pro Ser 
    50                  55                  60                  


Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser 
65                  70                  75                  80  


Ser Leu Gln Pro Glu Asp Phe Ala Thr Tyr Tyr Cys Gln Gln Ser Asn 
                85                  90                  95      


Glu Asp Pro Tyr Thr Phe Gly Gln Gly Thr Lys Leu Glu Ile Lys 
            100                 105                 110     


<210>  16
<211>  111
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  V_kappa_4

<400>  16

Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly 
1               5                   10                  15      


Asp Arg Val Thr Ile Thr Cys Lys Ala Ser Gln Ser Val Asp Tyr Asp 
            20                  25                  30          


Gly Asp Ser Tyr Met Lys Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro 
        35                  40                  45              


Lys Leu Leu Ile Tyr Ala Ala Ser Asn Leu Gln Ser Gly Val Pro Ser 
    50                  55                  60                  


Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser 
65                  70                  75                  80  


Ser Leu Gln Pro Glu Asp Phe Ala Thr Tyr Tyr Cys Gln Gln Ser Asn 
                85                  90                  95      


Glu Asp Pro Tyr Thr Phe Gly Gln Gly Thr Lys Leu Glu Ile Lys 
            100                 105                 110     


<210>  17
<211>  119
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Consensus sequence formed from the combination of VH1 to VH5


<220>
<221>  MISC_FEATURE
<222>  (9)..(9)
<223>  Xaa9 is A or P

<220>
<221>  MISC_FEATURE
<222>  (11)..(11)
<223>  Xaa11 is L or P

<220>
<221>  MISC_FEATURE
<222>  (20)..(20)
<223>  Xaa20 is I or V

<220>
<221>  MISC_FEATURE
<222>  (38)..(38)
<223>  Xaa38 is K or R

<220>
<221>  MISC_FEATURE
<222>  (48)..(48)
<223>  Xaa48 is I or M

<220>
<221>  MISC_FEATURE
<222>  (63)..(63)
<223>  Xaa63 is K or R

<220>
<221>  MISC_FEATURE
<222>  (68)..(68)
<223>  Xaa68 is A or V

<220>
<221>  MISC_FEATURE
<222>  (70)..(70)
<223>  Xaa70 is L or M

<220>
<221>  MISC_FEATURE
<222>  (82)..(82)
<223>  Xaa82 is E or Q

<220>
<221>  MISC_FEATURE
<222>  (86)..(86)
<223>  Xaa86 is L or P

<220>
<221>  MISC_FEATURE
<222>  (87)..(87)
<223>  Xaa87 is R or T

<220>
<221>  MISC_FEATURE
<222>  (91)..(91)
<223>  Xaa91 is S or T

<400>  17

Gln Val Gln Leu Val Gln Ser Gly Xaa Glu Xaa Lys Lys Pro Gly Ala 
1               5                   10                  15      


Ser Val Lys Xaa Ser Cys Lys Ala Ser Gly Tyr Ser Phe Thr Thr Phe 
            20                  25                  30          


Trp Met Asp Trp Val Xaa Gln Ala Pro Gly Gln Gly Leu Glu Trp Xaa 
        35                  40                  45              


Gly Arg Ile Asp Pro Ser Asp Ser Glu Ser Arg Leu Asp Gln Xaa Phe 
    50                  55                  60                  


Lys Asp Arg Xaa Thr Xaa Thr Val Asp Lys Ser Thr Ser Thr Val Tyr 
65                  70                  75                  80  


Met Xaa Leu Ser Ser Xaa Xaa Ser Glu Asp Xaa Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Gly Asn Asp Gly Tyr Tyr Gly Phe Ala Tyr Trp Gly Gln Gly 
            100                 105                 110         


Thr Leu Val Thr Val Ser Ser 
        115                 


<210>  18
<211>  111
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Consensus sequence formed from the combination of V_kappa_1 to 
       V_kappa_4


<220>
<221>  MISC_FEATURE
<222>  (3)..(3)
<223>  Xaa3 is V or Q

<220>
<221>  MISC_FEATURE
<222>  (4)..(4)
<223>  Xaa4 is L or M

<220>
<221>  MISC_FEATURE
<222>  (9)..(9)
<223>  Xaa9 is A or S

<220>
<221>  MISC_FEATURE
<222>  (12)..(12)
<223>  Xaa12 is A or S

<220>
<221>  MISC_FEATURE
<222>  (62)..(62)
<223>  Xaa62 is I or V

<220>
<221>  MISC_FEATURE
<222>  (84)..(84)
<223>  Xaa84 is E or P

<400>  18

Asp Ile Xaa Xaa Thr Gln Ser Pro Xaa Ser Leu Xaa Ala Ser Val Gly 
1               5                   10                  15      


Asp Arg Val Thr Ile Thr Cys Lys Ala Ser Gln Ser Val Asp Tyr Asp 
            20                  25                  30          


Gly Asp Ser Tyr Met Lys Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro 
        35                  40                  45              


Lys Leu Leu Ile Tyr Ala Ala Ser Asn Leu Gln Ser Gly Xaa Pro Ser 
    50                  55                  60                  


Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser 
65                  70                  75                  80  


Ser Leu Gln Xaa Glu Asp Phe Ala Thr Tyr Tyr Cys Gln Gln Ser Asn 
                85                  90                  95      


Glu Asp Pro Tyr Thr Phe Gly Gln Gly Thr Lys Leu Glu Ile Lys 
            100                 105                 110     


<210>  19
<211>  327
<212>  PRT
<213>  Homo sapiens

<400>  19

Ala Ser Thr Lys Gly Pro Ser Val Phe Pro Leu Ala Pro Cys Ser Arg 
1               5                   10                  15      


Ser Thr Ser Glu Ser Thr Ala Ala Leu Gly Cys Leu Val Lys Asp Tyr 
            20                  25                  30          


Phe Pro Glu Pro Val Thr Val Ser Trp Asn Ser Gly Ala Leu Thr Ser 
        35                  40                  45              


Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Ser Gly Leu Tyr Ser 
    50                  55                  60                  


Leu Ser Ser Val Val Thr Val Pro Ser Ser Ser Leu Gly Thr Lys Thr 
65                  70                  75                  80  


Tyr Thr Cys Asn Val Asp His Lys Pro Ser Asn Thr Lys Val Asp Lys 
                85                  90                  95      


Arg Val Glu Ser Lys Tyr Gly Pro Pro Cys Pro Ser Cys Pro Ala Pro 
            100                 105                 110         


Glu Phe Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys 
        115                 120                 125             


Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val 
    130                 135                 140                 


Asp Val Ser Gln Glu Asp Pro Glu Val Gln Phe Asn Trp Tyr Val Asp 
145                 150                 155                 160 


Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Phe 
                165                 170                 175     


Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp 
            180                 185                 190         


Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Gly Leu 
        195                 200                 205             


Pro Ser Ser Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg 
    210                 215                 220                 


Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Gln Glu Glu Met Thr Lys 
225                 230                 235                 240 


Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp 
                245                 250                 255     


Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys 
            260                 265                 270         


Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser 
        275                 280                 285             


Arg Leu Thr Val Asp Lys Ser Arg Trp Gln Glu Gly Asn Val Phe Ser 
    290                 295                 300                 


Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser 
305                 310                 315                 320 


Leu Ser Leu Ser Leu Gly Lys 
                325         


<210>  20
<211>  14
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  IFX-1 CDR1 heavy chain

<400>  20

Cys Lys Ala Ser Gly Tyr Ser Phe Thr Thr Phe Trp Met Asp 
1               5                   10                  


<210>  21
<211>  12
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  IFX-1 CDR2 heavy chain

<400>  21

Ile Asp Pro Ser Asp Ser Glu Ser Arg Leu Asp Gln 
1               5                   10          


<210>  22
<211>  13
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  IFX-1 CDR3 heavy chain

<400>  22

Cys Ala Arg Gly Asn Asp Gly Tyr Tyr Gly Phe Ala Tyr 
1               5                   10              


<210>  23
<211>  16
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  IFX-1 CDR1 light chain

<400>  23

Cys Lys Ala Ser Gln Ser Val Asp Tyr Asp Gly Asp Ser Tyr Met Lys 
1               5                   10                  15      


<210>  24
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  IFX-1 CDR2 light chain

<400>  24

Ile Tyr Ala Ala Ser Asn Leu 
1               5           


<210>  25
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  IFX-1 CDR3 light chain

<400>  25

Cys Gln Gln Ser Asn Glu Asp Pro Tyr Thr 
1               5                   10  


