                         SEQUENCE LISTING

<110>  Ohio State Innovation Foundation
 
<120>  METHODS AND COMPOSITIONS FOR ENHANCING STABILITY AND SOLUBILITY 
       OF SPLIT-INTEINS

<130>  10336-729WO1

<160>  18    

<170>  PatentIn version 3.5

<210>  1
<211>  102
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic construct

<400>  1

Cys Leu Ser Tyr Glu Thr Glu Ile Leu Thr Val Glu Tyr Gly Leu Leu 
1               5                   10                  15      


Pro Ile Gly Lys Ile Val Glu Lys Arg Ile Glu Cys Thr Val Tyr Ser 
            20                  25                  30          


Val Asp Asn Asn Gly Asn Ile Tyr Thr Gln Pro Val Ala Gln Trp His 
        35                  40                  45              


Asp Arg Gly Glu Gln Glu Val Phe Glu Tyr Cys Leu Glu Asp Gly Ser 
    50                  55                  60                  


Leu Ile Arg Ala Thr Lys Asp His Lys Phe Met Thr Val Asp Gly Gln 
65                  70                  75                  80  


Met Leu Pro Ile Asp Glu Ile Phe Glu Arg Glu Leu Asp Leu Met Arg 
                85                  90                  95      


Val Asp Asn Leu Pro Asn 
            100         


<210>  2
<211>  102
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  artificial sequence


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  Xaa can be any naturally occurring amino acid

<400>  2

Xaa Leu Ser Tyr Glu Thr Glu Ile Leu Thr Val Glu Tyr Gly Leu Leu 
1               5                   10                  15      


Pro Ile Gly Lys Ile Val Glu Lys Arg Ile Glu Cys Thr Val Tyr Ser 
            20                  25                  30          


Val Asp Asn Asn Gly Asn Ile Tyr Thr Gln Pro Val Ala Gln Trp His 
        35                  40                  45              


Asp Arg Gly Glu Gln Glu Val Phe Glu Tyr Cys Leu Glu Asp Gly Ser 
    50                  55                  60                  


Leu Ile Arg Ala Thr Lys Asp His Lys Phe Met Thr Val Asp Gly Gln 
65                  70                  75                  80  


Met Leu Pro Ile Asp Glu Ile Phe Glu Arg Glu Leu Asp Leu Met Arg 
                85                  90                  95      


Val Asp Asn Leu Pro Asn 
            100         


<210>  3
<211>  102
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  artificial construct


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  Xaa can be any naturally occurring amino acid

<400>  3

Xaa Leu Ser Tyr Glu Thr Glu Ile Leu Thr Val Glu Tyr Gly Leu Leu 
1               5                   10                  15      


Pro Ile Gly Lys Ile Val Glu Lys Arg Ile Glu Ser Thr Val Tyr Ser 
            20                  25                  30          


Val Asp Asn Asn Gly Asn Ile Tyr Thr Gln Pro Val Ala Gln Trp His 
        35                  40                  45              


Asp Arg Gly Glu Gln Glu Val Phe Glu Tyr Ser Leu Glu Asp Gly Ser 
    50                  55                  60                  


Leu Ile Arg Ala Thr Lys Asp His Lys Phe Met Thr Val Asp Gly Gln 
65                  70                  75                  80  


Met Leu Pro Ile Asp Glu Ile Phe Glu Arg Glu Leu Asp Leu Met Arg 
                85                  90                  95      


Val Asp Asn Leu Pro Asn 
            100         


<210>  4
<211>  108
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  artificial construct


<220>
<221>  misc_feature
<222>  (7)..(7)
<223>  Xaa can be any naturally occurring amino acid

<400>  4

Met Gly Asp Gly His Gly Xaa Leu Ser Tyr Glu Thr Glu Ile Leu Thr 
1               5                   10                  15      


Val Glu Tyr Gly Leu Leu Pro Ile Gly Lys Ile Val Glu Lys Arg Ile 
            20                  25                  30          


Glu Ser Thr Val Tyr Ser Val Asp Asn Asn Gly Asn Ile Tyr Thr Gln 
        35                  40                  45              


Pro Val Ala Gln Trp His Asp Arg Gly Glu Gln Glu Val Phe Glu Tyr 
    50                  55                  60                  


Ser Leu Glu Asp Gly Ser Leu Ile Arg Ala Thr Lys Asp His Lys Phe 
65                  70                  75                  80  


Met Thr Val Asp Gly Gln Met Leu Pro Ile Asp Glu Ile Phe Glu Arg 
                85                  90                  95      


Glu Leu Asp Leu Met Arg Val Asp Asn Leu Pro Asn 
            100                 105             


<210>  5
<211>  120
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  artificial construct


<220>
<221>  misc_feature
<222>  (7)..(7)
<223>  Xaa can be any naturally occurring amino acid

<400>  5

Met Gly Asp Gly His Gly Xaa Leu Ser Tyr Glu Thr Glu Ile Leu Thr 
1               5                   10                  15      


Val Glu Tyr Gly Leu Leu Pro Ile Gly Lys Ile Val Glu Lys Arg Ile 
            20                  25                  30          


Glu Ser Thr Val Tyr Ser Val Asp Asn Asn Gly Asn Ile Tyr Thr Gln 
        35                  40                  45              


Pro Val Ala Gln Trp His Asp Arg Gly Glu Gln Glu Val Phe Glu Tyr 
    50                  55                  60                  


Ser Leu Glu Asp Gly Ser Leu Ile Arg Ala Thr Lys Asp His Lys Phe 
65                  70                  75                  80  


Met Thr Val Asp Gly Gln Met Leu Pro Ile Asp Glu Ile Phe Glu Arg 
                85                  90                  95      


Glu Leu Asp Leu Met Arg Val Asp Asn Leu Pro Asn Gly Gly Gly Gly 
            100                 105                 110         


Ser His His His His His His Cys 
        115                 120 


<210>  6
<211>  120
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  artificial construct

<400>  6

Met Gly Asp Gly His Gly Ala Leu Ser Tyr Glu Thr Glu Ile Leu Thr 
1               5                   10                  15      


Val Glu Tyr Gly Leu Leu Pro Ile Gly Lys Ile Val Glu Lys Arg Ile 
            20                  25                  30          


Glu Ser Thr Val Tyr Ser Val Asp Asn Asn Gly Asn Ile Tyr Thr Gln 
        35                  40                  45              


Pro Val Ala Gln Trp His Asp Arg Gly Glu Gln Glu Val Phe Glu Tyr 
    50                  55                  60                  


Ser Leu Glu Asp Gly Ser Leu Ile Arg Ala Thr Lys Asp His Lys Phe 
65                  70                  75                  80  


Met Thr Val Asp Gly Gln Met Leu Pro Ile Asp Glu Ile Phe Glu Arg 
                85                  90                  95      


Glu Leu Asp Leu Met Arg Val Asp Asn Leu Pro Asn Gly Gly Gly Gly 
            100                 105                 110         


Ser Cys His His His His His His 
        115                 120 


<210>  7
<211>  125
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  artificial construct

<400>  7

Met Gly Asp Gly His Gly Ala Leu Ser Tyr Glu Thr Glu Ile Leu Thr 
1               5                   10                  15      


Val Glu Tyr Gly Leu Leu Pro Ile Gly Lys Ile Val Glu Lys Arg Ile 
            20                  25                  30          


Glu Ser Thr Val Tyr Ser Val Asp Asn Asn Gly Asn Ile Tyr Thr Gln 
        35                  40                  45              


Pro Val Ala Gln Trp His Asp Arg Gly Glu Gln Glu Val Phe Glu Tyr 
    50                  55                  60                  


Ser Leu Glu Asp Gly Ser Leu Ile Arg Ala Thr Lys Asp His Lys Phe 
65                  70                  75                  80  


Met Thr Val Asp Gly Gln Met Leu Pro Ile Asp Glu Ile Phe Glu Arg 
                85                  90                  95      


Glu Leu Asp Leu Met Arg Val Asp Asn Leu Pro Asn Gly Gly Gly Gly 
            100                 105                 110         


Ser Cys Gly Gly Gly Gly Ser His His His His His His 
        115                 120                 125 


<210>  8
<211>  130
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  artificial construct

<400>  8

Met Gly Asp Gly His Gly Ala Leu Ser Tyr Glu Thr Glu Ile Leu Thr 
1               5                   10                  15      


Val Glu Tyr Gly Leu Leu Pro Ile Gly Lys Ile Val Glu Lys Arg Ile 
            20                  25                  30          


Glu Ser Thr Val Tyr Ser Val Asp Asn Asn Gly Asn Ile Tyr Thr Gln 
        35                  40                  45              


Pro Val Ala Gln Trp His Asp Arg Gly Glu Gln Glu Val Phe Glu Tyr 
    50                  55                  60                  


Ser Leu Glu Asp Gly Ser Leu Ile Arg Ala Thr Lys Asp His Lys Phe 
65                  70                  75                  80  


Met Thr Val Asp Gly Gln Met Leu Pro Ile Asp Glu Ile Phe Glu Arg 
                85                  90                  95      


Glu Leu Asp Leu Met Arg Val Asp Asn Leu Pro Asn Gly Gly Gly Gly 
            100                 105                 110         


Ser Gly Gly Gly Gly Ser Cys Gly Gly Gly Gly Ser His His His His 
        115                 120                 125             


His His 
    130 


<210>  9
<211>  124
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  artificial construct

<400>  9

Met Gly Asp Gly His Gly Ala Leu Ser Tyr Glu Thr Glu Ile Leu Thr 
1               5                   10                  15      


Val Glu Tyr Gly Leu Leu Pro Ile Gly Lys Ile Val Glu Lys Arg Ile 
            20                  25                  30          


Glu Ser Thr Val Tyr Ser Val Asp Asn Asn Gly Asn Ile Tyr Thr Gln 
        35                  40                  45              


Pro Val Ala Gln Trp His Asp Arg Gly Glu Gln Glu Val Phe Glu Tyr 
    50                  55                  60                  


Ser Leu Glu Asp Gly Ser Leu Ile Arg Ala Thr Lys Asp His Lys Phe 
65                  70                  75                  80  


Met Thr Val Asp Gly Gln Met Leu Pro Ile Asp Glu Ile Phe Glu Arg 
                85                  90                  95      


Glu Leu Asp Leu Met Arg Val Asp Asn Leu Pro Asn Gly Gly Gly Gly 
            100                 105                 110         


Ser Gly Gly Gly Gly Ser Cys Gly Gly Gly Gly Ser 
        115                 120                 


<210>  10
<211>  36
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  artificial construct

<400>  10

Met Ile Lys Ile Ala Thr Arg Lys Tyr Leu Gly Lys Gln Asn Val Tyr 
1               5                   10                  15      


Asp Ile Gly Val Glu Arg Asp His Asn Phe Ala Leu Lys Asn Gly Phe 
            20                  25                  30          


Ile Ala Ser Asn 
        35      


<210>  11
<211>  36
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  artificial construct

<400>  11

Met Ile Lys Ile Ala Thr Arg Lys Tyr Leu Gly Lys Gln Asn Val Tyr 
1               5                   10                  15      


Gly Ile Gly Val Glu Arg Asp His Asn Phe Ala Leu Lys Asn Gly Phe 
            20                  25                  30          


Ile Ala Ser Asn 
        35      


<210>  12
<211>  36
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  artificial construct

<400>  12

Met Ile Lys Ile Ala Thr Arg Lys Tyr Leu Gly Lys Gln Asn Val Tyr 
1               5                   10                  15      


Gly Ile Gly Val Glu Arg Asp His Asn Phe Ala Leu Lys Asn Gly Phe 
            20                  25                  30          


Ile Ala His Asn 
        35      


<210>  13
<211>  287
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  artificial construct

<400>  13

Met Ile Lys Ile Ala Thr Arg Lys Tyr Leu Gly Lys Gln Asn Val Tyr 
1               5                   10                  15      


Gly Ile Gly Val Glu Arg Asp His Asn Phe Ala Leu Lys Asn Gly Phe 
            20                  25                  30          


Ile Ala His Asn Phe Phe Asn Gly Thr Val Ser Lys Gly Glu Glu Leu 
        35                  40                  45              


Phe Thr Gly Val Val Pro Ile Leu Val Glu Leu Asp Gly Asp Val Asn 
    50                  55                  60                  


Gly His Lys Phe Ser Val Arg Gly Glu Gly Glu Gly Asp Ala Thr Asn 
65                  70                  75                  80  


Gly Lys Leu Thr Leu Lys Phe Ile Cys Thr Thr Gly Lys Leu Pro Val 
                85                  90                  95      


Pro Trp Pro Thr Leu Val Thr Thr Leu Thr Tyr Gly Val Gln Cys Phe 
            100                 105                 110         


Ser Arg Tyr Pro Asp His Met Lys Gln His Asp Phe Phe Lys Ser Ala 
        115                 120                 125             


Met Pro Glu Gly Tyr Val Gln Glu Arg Thr Ile Ser Phe Lys Asp Asp 
    130                 135                 140                 


Gly Thr Tyr Lys Thr Arg Ala Glu Val Lys Phe Glu Gly Asp Thr Leu 
145                 150                 155                 160 


Val Asn Arg Ile Glu Leu Lys Gly Ile Asp Phe Lys Glu Asp Gly Asn 
                165                 170                 175     


Ile Leu Gly His Lys Leu Glu Tyr Asn Phe Asn Ser His Asn Val Tyr 
            180                 185                 190         


Ile Thr Ala Asp Lys Gln Lys Asn Gly Ile Lys Ala Asn Phe Lys Ile 
        195                 200                 205             


Arg His Asn Val Glu Asp Gly Ser Val Gln Leu Ala Asp His Tyr Gln 
    210                 215                 220                 


Gln Asn Thr Pro Ile Gly Asp Gly Pro Val Leu Leu Pro Asp Asn His 
225                 230                 235                 240 


Tyr Leu Ser Thr Gln Ser Lys Leu Ser Lys Asp Pro Asn Glu Lys Arg 
                245                 250                 255     


Asp His Met Val Leu Leu Glu Phe Val Thr Ala Ala Gly Ile Thr Leu 
            260                 265                 270         


Gly Met Asp Glu Leu Tyr Lys Leu Glu His His His His His His 
        275                 280                 285         


<210>  14
<211>  36
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  artificial construct

<400>  14

Met Ile Lys Ile Ala Thr Arg Lys Tyr Leu Gly Lys Gln Asn Val Tyr 
1               5                   10                  15      


Gly Ile Gly Val Glu Arg Asp His Asn Phe Ala Leu Lys Asn Gly Phe 
            20                  25                  30          


Ile Ala His Ala 
        35      


<210>  15
<211>  42
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  artificial construct

<400>  15

Met His His His His His His Ile Lys Ile Ala Thr Arg Lys Tyr Leu 
1               5                   10                  15      


Gly Lys Gln Asn Val Tyr Gly Ile Gly Val Glu Arg Asp His Asn Phe 
            20                  25                  30          


Ala Leu Lys Asn Gly Phe Ile Ala His Ala 
        35                  40          


<210>  16
<211>  42
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  artificial construct

<400>  16

Met Ile Lys Ile Ala Thr Arg Lys Tyr Leu Gly Lys Gln Asn Val Tyr 
1               5                   10                  15      


Gly Ile Gly Val Glu Arg Asp His Asn Phe Ala Leu Lys Asn Gly Phe 
            20                  25                  30          


Ile Ala His Ala His His His His His His 
        35                  40          


<210>  17
<211>  287
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  artificial construct

<400>  17

Met Ile Lys Ile Ala Thr Arg Lys Tyr Leu Gly Lys Gln Asn Val Tyr 
1               5                   10                  15      


Gly Ile Gly Val Glu Arg Asp His Asn Phe Ala Leu Lys Asn Gly Phe 
            20                  25                  30          


Ile Ala His Asn Met Phe Asn Gly Thr Val Ser Lys Gly Glu Glu Leu 
        35                  40                  45              


Phe Thr Gly Val Val Pro Ile Leu Val Glu Leu Asp Gly Asp Val Asn 
    50                  55                  60                  


Gly His Lys Phe Ser Val Arg Gly Glu Gly Glu Gly Asp Ala Thr Asn 
65                  70                  75                  80  


Gly Lys Leu Thr Leu Lys Phe Ile Cys Thr Thr Gly Lys Leu Pro Val 
                85                  90                  95      


Pro Trp Pro Thr Leu Val Thr Thr Leu Thr Tyr Gly Val Gln Cys Phe 
            100                 105                 110         


Ser Arg Tyr Pro Asp His Met Lys Gln His Asp Phe Phe Lys Ser Ala 
        115                 120                 125             


Met Pro Glu Gly Tyr Val Gln Glu Arg Thr Ile Ser Phe Lys Asp Asp 
    130                 135                 140                 


Gly Thr Tyr Lys Thr Arg Ala Glu Val Lys Phe Glu Gly Asp Thr Leu 
145                 150                 155                 160 


Val Asn Arg Ile Glu Leu Lys Gly Ile Asp Phe Lys Glu Asp Gly Asn 
                165                 170                 175     


Ile Leu Gly His Lys Leu Glu Tyr Asn Phe Asn Ser His Asn Val Tyr 
            180                 185                 190         


Ile Thr Ala Asp Lys Gln Lys Asn Gly Ile Lys Ala Asn Phe Lys Ile 
        195                 200                 205             


Arg His Asn Val Glu Asp Gly Ser Val Gln Leu Ala Asp His Tyr Gln 
    210                 215                 220                 


Gln Asn Thr Pro Ile Gly Asp Gly Pro Val Leu Leu Pro Asp Asn His 
225                 230                 235                 240 


Tyr Leu Ser Thr Gln Ser Lys Leu Ser Lys Asp Pro Asn Glu Lys Arg 
                245                 250                 255     


Asp His Met Val Leu Leu Glu Phe Val Thr Ala Ala Gly Ile Thr Leu 
            260                 265                 270         


Gly Met Asp Glu Leu Tyr Lys Leu Glu His His His His His His 
        275                 280                 285         


<210>  18
<211>  120
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  artificial construct


<220>
<221>  misc_feature
<222>  (7)..(7)
<223>  Xaa can be any naturally occurring amino acid

<400>  18

Met Gly Asp Gly His Gly Xaa Leu Ser Tyr Glu Thr Glu Ile Leu Thr 
1               5                   10                  15      


Val Glu Tyr Gly Leu Leu Pro Ile Gly Lys Ile Val Glu Lys Arg Ile 
            20                  25                  30          


Glu Ser Thr Val Tyr Ser Val Asp Asn Asn Gly Asn Ile Tyr Thr Gln 
        35                  40                  45              


Pro Val Ala Gln Trp His Asp Arg Gly Glu Gln Glu Val Phe Glu Tyr 
    50                  55                  60                  


Ser Leu Glu Asp Gly Ser Leu Ile Arg Ala Thr Lys Asp His Lys Phe 
65                  70                  75                  80  


Met Thr Val Asp Gly Gln Met Leu Pro Ile Asp Glu Ile Phe Glu Arg 
                85                  90                  95      


Glu Leu Asp Leu Met Arg Val Asp Asn Leu Pro Asn Gly Gly Gly Gly 
            100                 105                 110         


Ser Cys His His His His His His 
        115                 120 


