                         SEQUENCE LISTING

<110>  Temple University
       Nicholson, Allen
       Stopar, Alex
       Castronovo, Matteo
 
<120>  Epiprinter Technology and Methods of Use for Detecting 
       Biomolecules

<130>  206017-0192-00WO

<150>  US 63/017,819
<151>  2020-04-30

<160>  27    

<170>  PatentIn version 3.5

<210>  1
<211>  228
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Chemically Synthesized, HBD

<400>  1
atgggcagca gccatcatca tcatcatcac agcagcggcc tggtgccgcg cggcagccat       60

atgaagttgg caaaaaaata ctacgctgta agaaaaggaa gagttcccgg gatctacaaa      120

acctggaaag aggcggaaga acaggtaaaa ggtttcccgg gagccgagta caaaagtttt      180

gaaagactgg aagacgcgaa agcctatatg gaaggaaaga acgagtga                   228


<210>  2
<211>  75
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Chemically Synthesized, HBD

<400>  2

Met Gly Ser Ser His His His His His His Ser Ser Gly Leu Val Pro 
1               5                   10                  15      


Arg Gly Ser His Met Lys Leu Ala Lys Lys Tyr Tyr Ala Val Arg Lys 
            20                  25                  30          


Gly Arg Val Pro Gly Ile Tyr Lys Thr Trp Lys Glu Ala Glu Glu Gln 
        35                  40                  45              


Val Lys Gly Phe Pro Gly Ala Glu Tyr Lys Ser Phe Glu Arg Leu Glu 
    50                  55                  60                  


Asp Ala Lys Ala Tyr Met Glu Gly Lys Asn Glu 
65                  70                  75  


<210>  3
<211>  35
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Chemically Synthesized, HBD 5' PCR primer

<400>  3
gggaattcca tatgaagttg gcaaaaaaat actac                                  35


<210>  4
<211>  39
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Chemically Synthesized, HBD 3' PCR primer

<400>  4
gggaatggat cctcactcgt tctttccttc catataggc                              39


<210>  5
<211>  229
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Chemically Synthesized, HBD1-2S

<400>  5
gggaatgggg tctcacgtct ctgtgtccag ttctgggctg atgctctcgt tctttccttc       60

catataggct ttcgcgtctt ccagtctttc aaaacttttg tactcggctc ccgggaaacc      120

ttttacctgt tcttccgcct ctttccaggt tttgtagatc ccgggaactc ttccttttct      180

tacagcgtag tatttttttg ccaacttcat atgcgagacc ggaattccc                  229


<210>  6
<211>  208
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Chemically Synthesized, HBD2 with a stop codon

<400>  6
gggaatgggg tctcatcctc tcactcgttc tttccttcca tataggcttt cgcgtcttcc       60

agtctttcaa aacttttgta ctcggctccc gggaaacctt ttacctgttc ttccgcctct      120

ttccaggttt tgtagatccc gggaactctt ccttttctta cagcgtagta tttttttgcc      180

aacttcatcg tccgagaccg gaattccc                                         208


<210>  7
<211>  423
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Chemically Synthesized, DHBD

<400>  7
atgggcagca gccatcatca tcatcatcac agcagcggcc tggtgccgcg cggcagccat       60

atgaagttgg caaaaaaata ctacgctgta agaaaaggaa gagttcccgg gatctacaaa      120

acctggaaag aggcggaaga acaggtaaaa ggtttcccgg gagccgagta caaaagtttt      180

gaaagactgg aagacgcgaa agcctatatg gaaggaaaga acgagagcat cagcccagaa      240

ctggacacag agacgatgaa gttggcaaaa aaatactacg ctgtaagaaa aggaagagtt      300

cccgggatct acaaaacctg gaaagaggcg gaagaacagg taaaaggttt cccgggagcc      360

gagtacaaaa gttttgaaag actggaagac gcgaaagcct atatggaagg aaagaacgag      420

tga                                                                    423


<210>  8
<211>  140
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Chemically Synthesized, DHBD

<400>  8

Met Gly Ser Ser His His His His His His Ser Ser Gly Leu Val Pro 
1               5                   10                  15      


Arg Gly Ser His Met Lys Leu Ala Lys Lys Tyr Tyr Ala Val Arg Lys 
            20                  25                  30          


Gly Arg Val Pro Gly Ile Tyr Lys Thr Trp Lys Glu Ala Glu Glu Gln 
        35                  40                  45              


Val Lys Gly Phe Pro Gly Ala Glu Tyr Lys Ser Phe Glu Arg Leu Glu 
    50                  55                  60                  


Asp Ala Lys Ala Tyr Met Glu Gly Lys Asn Glu Ser Ile Ser Pro Glu 
65                  70                  75                  80  


Leu Asp Thr Glu Thr Met Lys Leu Ala Lys Lys Tyr Tyr Ala Val Arg 
                85                  90                  95      


Lys Gly Arg Val Pro Gly Ile Tyr Lys Thr Trp Lys Glu Ala Glu Glu 
            100                 105                 110         


Gln Val Lys Gly Phe Pro Gly Ala Glu Tyr Lys Ser Phe Glu Arg Leu 
        115                 120                 125             


Glu Asp Ala Lys Ala Tyr Met Glu Gly Lys Asn Glu 
    130                 135                 140 


<210>  9
<211>  229
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Chemically Synthesized, HBD1-2S

<400>  9
gggaatgggg tctcacgtct ctgtgtccag ttctgggctg atgctctcgt tctttccttc       60

catataggct ttcgcgtctt ccagtctttc aaaacttttg tactcggctc ccgggaaacc      120

ttttacctgt tcttccgcct ctttccaggt tttgtagatc ccgggaactc ttccttttct      180

tacagcgtag tatttttttg ccaacttcat atgcgagacc ggaattccc                  229


<210>  10
<211>  221
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Chemically Synthesized, HBD1-1C

<400>  10
gggaatgggg tctcagtcca gttctgggct gatgctctcg ttctttcctt ccatataggc       60

tttcgcgtct tccagtcttt caaaactttt gtactcggct cccgggaaac cttttacctg      120

ttcttccgcc tctttccagg ttttgtagat cccgggaact cttccttttc ttacagcgta      180

gtattttttt gccaacttca tcgtccgaga ccggaattcc c                          221


<210>  11
<211>  208
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Chemically Synthesized, HBD2-2S

<400>  11
gggaatgggg tctcatcctc tcactcgttc tttccttcca tataggcttt cgcgtcttcc       60

agtctttcaa aacttttgta ctcggctccc gggaaacctt ttacctgttc ttccgcctct      120

ttccaggttt tgtagatccc gggaactctt ccttttctta cagcgtagta tttttttgcc      180

aacttcatcg tccgagaccg gaattccc                                         208


<210>  12
<211>  217
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Chemically Synthesized, HBD3 with stop codon

<400>  12
gggaatgggg tctcatcctc tcactcgttc tttccttcca tataggcttt cgcgtcttcc       60

agtctttcaa aacttttgta ctcggctccc gggaaacctt ttacctgttc ttccgcctct      120

ttccaggttt tgtagatccc gggaactctt ccttttctta cagcgtagta tttttttgcc      180

aacttcatcg tctctgtgtc ccgagaccgg aattccc                               217


<210>  13
<211>  618
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Chemically Synthesized, THBD

<400>  13
atgggcagca gccatcatca tcatcatcac agcagcggcc tggtgccgcg cggcagccat       60

atgaagttgg caaaaaaata ctacgctgta agaaaaggaa gagttcccgg gatctacaaa      120

acctggaaag aggcggaaga acaggtaaaa ggtttcccgg gagccgagta caaaagtttt      180

gaaagactgg aagacgcgaa agcctatatg gaaggaaaga acgagagcat cagcccagaa      240

ctggacacag agacgatgaa gttggcaaaa aaatactacg ctgtaagaaa aggaagagtt      300

cccgggatct acaaaacctg gaaagaggcg gaagaacagg taaaaggttt cccgggagcc      360

gagtacaaaa gttttgaaag actggaagac gcgaaagcct atatggaagg aaagaacgag      420

agcatcagcc cagaactgga cacagagacg atgaagttgg caaaaaaata ctacgctgta      480

agaaaaggaa gagttcccgg gatctacaaa acctggaaag aggcggaaga acaggtaaaa      540

ggtttcccgg gagccgagta caaaagtttt gaaagactgg aagacgcgaa agcctatatg      600

gaaggaaaga acgagtga                                                    618


<210>  14
<211>  205
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Chemically Synthesized, THBD

<400>  14

Met Gly Ser Ser His His His His His His Ser Ser Gly Leu Val Pro 
1               5                   10                  15      


Arg Gly Ser His Met Lys Leu Ala Lys Lys Tyr Tyr Ala Val Arg Lys 
            20                  25                  30          


Gly Arg Val Pro Gly Ile Tyr Lys Thr Trp Lys Glu Ala Glu Glu Gln 
        35                  40                  45              


Val Lys Gly Phe Pro Gly Ala Glu Tyr Lys Ser Phe Glu Arg Leu Glu 
    50                  55                  60                  


Asp Ala Lys Ala Tyr Met Glu Gly Lys Asn Glu Ser Ile Ser Pro Glu 
65                  70                  75                  80  


Leu Asp Thr Glu Thr Met Lys Leu Ala Lys Lys Tyr Tyr Ala Val Arg 
                85                  90                  95      


Lys Gly Arg Val Pro Gly Ile Tyr Lys Thr Trp Lys Glu Ala Glu Glu 
            100                 105                 110         


Gln Val Lys Gly Phe Pro Gly Ala Glu Tyr Lys Ser Phe Glu Arg Leu 
        115                 120                 125             


Glu Asp Ala Lys Ala Tyr Met Glu Gly Lys Asn Glu Ser Ile Ser Pro 
    130                 135                 140                 


Glu Leu Asp Thr Glu Thr Met Lys Leu Ala Lys Lys Tyr Tyr Ala Val 
145                 150                 155                 160 


Arg Lys Gly Arg Val Pro Gly Ile Tyr Lys Thr Trp Lys Glu Ala Glu 
                165                 170                 175     


Glu Gln Val Lys Gly Phe Pro Gly Ala Glu Tyr Lys Ser Phe Glu Arg 
            180                 185                 190         


Leu Glu Asp Ala Lys Ala Tyr Met Glu Gly Lys Asn Glu 
        195                 200                 205 


<210>  15
<211>  618
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Chemically Synthesized, THBD-1C

<400>  15
atgggcagca gccatcatca tcatcatcac agcagcggcc tggtgccgcg cggcagccat       60

atgaagttgg caaaaaaata ctacgctgta agaaaaggaa gagttcccgg gatctacaaa      120

acctggaaag aggcggaaga acaggtaaaa ggtttcccgg gagccgagta caaaagtttt      180

gaaagactgg aagacgcgaa agcctatatg gaaggaaaga acgagagcat ctgtccagaa      240

ctggacacag agacgatgaa gttggcaaaa aaatactacg ctgtaagaaa aggaagagtt      300

cccgggatct acaaaacctg gaaagaggcg gaagaacagg taaaaggttt cccgggagcc      360

gagtacaaaa gttttgaaag actggaagac gcgaaagcct atatggaagg aaagaacgag      420

agcatcagcc cagaactgga cacagagacg atgaagttgg caaaaaaata ctacgctgta      480

agaaaaggaa gagttcccgg gatctacaaa acctggaaag aggcggaaga acaggtaaaa      540

ggtttcccgg gagccgagta caaaagtttt gaaagactgg aagacgcgaa agcctatatg      600

gaaggaaaga acgagtga                                                    618


<210>  16
<211>  185
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Chemically Synthesized, THBD-1C

<400>  16

Met Lys Leu Ala Lys Lys Tyr Tyr Ala Val Arg Lys Gly Arg Val Pro 
1               5                   10                  15      


Gly Ile Tyr Lys Thr Trp Lys Glu Ala Glu Glu Gln Val Lys Gly Phe 
            20                  25                  30          


Pro Gly Ala Glu Tyr Lys Ser Phe Glu Arg Leu Glu Asp Ala Lys Ala 
        35                  40                  45              


Tyr Met Glu Gly Lys Asn Glu Ser Ile Cys Pro Glu Leu Asp Thr Glu 
    50                  55                  60                  


Thr Met Lys Leu Ala Lys Lys Tyr Tyr Ala Val Arg Lys Gly Arg Val 
65                  70                  75                  80  


Pro Gly Ile Tyr Lys Thr Trp Lys Glu Ala Glu Glu Gln Val Lys Gly 
                85                  90                  95      


Phe Pro Gly Ala Glu Tyr Lys Ser Phe Glu Arg Leu Glu Asp Ala Lys 
            100                 105                 110         


Ala Tyr Met Glu Gly Lys Asn Glu Ser Ile Ser Pro Glu Leu Asp Thr 
        115                 120                 125             


Glu Thr Met Lys Leu Ala Lys Lys Tyr Tyr Ala Val Arg Lys Gly Arg 
    130                 135                 140                 


Val Pro Gly Ile Tyr Lys Thr Trp Lys Glu Ala Glu Glu Gln Val Lys 
145                 150                 155                 160 


Gly Phe Pro Gly Ala Glu Tyr Lys Ser Phe Glu Arg Leu Glu Asp Ala 
                165                 170                 175     


Lys Ala Tyr Met Glu Gly Lys Asn Glu 
            180                 185 


<210>  17
<211>  229
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Chemically Synthesized, HBD linked to a 2C linker

<400>  17
gggaatgggg tctcacgtct ctgtgtccag ttctggacag atgcactcgt tctttccttc       60

catataggct ttcgcgtctt ccagtctttc aaaacttttg tactcggctc ccgggaaacc      120

ttttacctgt tcttccgcct ctttccaggt tttgtagatc ccgggaactc ttccttttct      180

tacagcgtag tatttttttg ccaacttcat atgcgagacc ggaattccc                  229


<210>  18
<211>  423
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Chemically Synthesized, DHBD-2C

<400>  18
atgggcagca gccatcatca tcatcatcac agcagcggcc tggtgccgcg cggcagccat       60

atgaagttgg caaaaaaata ctacgctgta agaaaaggaa gagttcccgg gatctacaaa      120

acctggaaag aggcggaaga acaggtaaaa ggtttcccgg gagccgagta caaaagtttt      180

gaaagactgg aagacgcgaa agcctatatg gaaggaaaga acgagtgcat ctgtccagaa      240

ctggacacag agacgatgaa gttggcaaaa aaatactacg ctgtaagaaa aggaagagtt      300

cccgggatct acaaaacctg gaaagaggcg gaagaacagg taaaaggttt cccgggagcc      360

gagtacaaaa gttttgaaag actggaagac gcgaaagcct atatggaagg aaagaacgag      420

tga                                                                    423


<210>  19
<211>  140
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Chemically Synthesized, DHBD-2C

<400>  19

Met Gly Ser Ser His His His His His His Ser Ser Gly Leu Val Pro 
1               5                   10                  15      


Arg Gly Ser His Met Lys Leu Ala Lys Lys Tyr Tyr Ala Val Arg Lys 
            20                  25                  30          


Gly Arg Val Pro Gly Ile Tyr Lys Thr Trp Lys Glu Ala Glu Glu Gln 
        35                  40                  45              


Val Lys Gly Phe Pro Gly Ala Glu Tyr Lys Ser Phe Glu Arg Leu Glu 
    50                  55                  60                  


Asp Ala Lys Ala Tyr Met Glu Gly Lys Asn Glu Cys Ile Cys Pro Glu 
65                  70                  75                  80  


Leu Asp Thr Glu Thr Met Lys Leu Ala Lys Lys Tyr Tyr Ala Val Arg 
                85                  90                  95      


Lys Gly Arg Val Pro Gly Ile Tyr Lys Thr Trp Lys Glu Ala Glu Glu 
            100                 105                 110         


Gln Val Lys Gly Phe Pro Gly Ala Glu Tyr Lys Ser Phe Glu Arg Leu 
        115                 120                 125             


Glu Asp Ala Lys Ala Tyr Met Glu Gly Lys Asn Glu 
    130                 135                 140 


<210>  20
<211>  55
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Chemically Synthesized, hybrid binding domain

<400>  20

Met Lys Leu Ala Lys Lys Tyr Tyr Ala Val Arg Lys Gly Arg Val Pro 
1               5                   10                  15      


Gly Ile Tyr Lys Thr Trp Lys Glu Ala Glu Glu Gln Val Lys Gly Phe 
            20                  25                  30          


Pro Gly Ala Glu Tyr Lys Ser Phe Glu Arg Leu Glu Asp Ala Lys Ala 
        35                  40                  45              


Tyr Met Glu Gly Lys Asn Glu 
    50                  55  


<210>  21
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Chemically Synthesized, Linker-S

<400>  21

Ser Ile Ser Pro Glu Leu Asp Thr Glu Thr 
1               5                   10  


<210>  22
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Chemically Synthesized, Linker-C

<400>  22

Ser Ile Cys Pro Glu Leu Asp Thr Glu Thr 
1               5                   10  


<210>  23
<211>  47
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Chemically Synthesized, 21 bp DNA-RNA hairpin used for the SPR 
       analyses

<400>  23
tcaacgtttc ggaccgtatt tcttttgaaa uacgguccga aacguug                     47


<210>  24
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Chemically Synthesized, DNA portion of the 21 bp DNA-RNA hairpin 
       used for the SPR analyses

<400>  24
caacgtttcg gaccgtattt c                                                 21


<210>  25
<211>  21
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Chemically Synthesized, RNA portion of the 21 bp DNA-RNA hairpin 
       used for the SPR analyses

<400>  25
gaaauacggu ccgaaacguu g                                                 21


<210>  26
<211>  11
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Chemically Synthesized, , BsaI cleavage site


<220>
<221>  misc_feature
<222>  (1)..(5)
<223>  any nuclotide

<400>  26
nnnnngagac c                                                            11


<210>  27
<211>  11
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Chemincally Synthesized, BsaI cleavage site


<220>
<221>  misc_feature
<222>  (7)..(11)
<223>  any nucleotide

<400>  27
ggtctcnnnn n                                                            11


