                         SEQUENCE LISTING

<110>  Primex Clinical Laboratories
       Manucharyan, Karen
 
<120>  Methods of Generating Vaccines Against Novel Coronavirus, Named 
       SARS-CoV-2 Comprising Variable Epitope Libraries (VELs) As 
       Immunogens

<130>  42740-11404

<150>  US 63/058,890
<151>  2020-07-30

<150>  US 63/018,814
<151>  2020-05-01

<160>  24    

<170>  PatentIn version 3.5

<210>  1
<211>  25
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  2019-nCoV env protein fragment

<400>  1

Ile Val Asn Ser Tyr Leu Leu Phe Leu Ala Phe Val Val Phe Leu Leu 
1               5                   10                  15      


Val Thr Leu Ala Ile Leu Thr Ala Leu 
            20                  25  


<210>  2
<211>  25
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CoVV1 VEL Vaccine


<220>
<221>  MISC_FEATURE
<222>  (7)..(7)
<223>  X = any out of 20 proteinogenic amino acids

<220>
<221>  MISC_FEATURE
<222>  (12)..(12)
<223>  X = any out of 20 proteinogenic amino acids

<220>
<221>  MISC_FEATURE
<222>  (20)..(20)
<223>  X = any out of 20 proteinogenic amino acids

<400>  2

Ile Val Asn Ser Val Leu Xaa Phe Leu Ala Phe Xaa Val Phe Leu Leu 
1               5                   10                  15      


Val Thr Leu Xaa Ile Leu Thr Ala Leu 
            20                  25  


<210>  3
<211>  28
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  2019-nCoV Env protein fragment

<400>  3

Ala Ile Leu Thr Ala Leu Arg Leu Cys Ala Tyr Cys Cys Asn Ile Val 
1               5                   10                  15      


Asn Val Ser Leu Val Lys Pro Ser Phe Tyr Val Tyr 
            20                  25              


<210>  4
<211>  28
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CoVV2 VEL Vaccine


<220>
<221>  misc_feature
<222>  (5)..(5)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (12)..(12)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (17)..(17)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (24)..(24)
<223>  Xaa can be any naturally occurring amino acid

<400>  4

Ala Ile Leu Thr Xaa Leu Arg Leu Cys Ala Tyr Xaa Cys Asn Ile Val 
1               5                   10                  15      


Xaa Val Ser Leu Val Lys Pro Xaa Phe Tyr Val Tyr 
            20                  25              


<210>  5
<211>  21
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  2019-nCoV Membrane glycoprotein fragment

<400>  5

Phe Leu Trp Leu Leu Trp Pro Val Thr Leu Ala Cys Phe Val Leu Ala 
1               5                   10                  15      


Ala Val Tyr Arg Ile 
            20      


<210>  6
<211>  23
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CoVV3 VEL Vaccine


<220>
<221>  MISC_FEATURE
<222>  (5)..(5)
<223>  X = any out of 20 proteinogenic amino acids

<220>
<221>  MISC_FEATURE
<222>  (13)..(13)
<223>  X = any out of 20 proteinogenic amino acids

<220>
<221>  MISC_FEATURE
<222>  (18)..(18)
<223>  X = any out of 20 proteinogenic amino acids

<400>  6

Phe Leu Ile Asn Xaa Leu Ile Cys Pro Val Thr Leu Xaa Cys Phe Val 
1               5                   10                  15      


Leu Xaa Ala Val Tyr Arg Ile 
            20              


<210>  7
<211>  13
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  2019-nCoV Membrane glycoprotein fragment

<400>  7

Thr Val Ala Thr Ser Arg Thr Leu Ser Tyr Tyr Lys Leu 
1               5                   10              


<210>  8
<211>  13
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CoVV4  VEL Vaccine


<220>
<221>  MISC_FEATURE
<222>  (3)..(3)
<223>  X = any out of 20 proteinogenic amino acids

<220>
<221>  MISC_FEATURE
<222>  (7)..(7)
<223>  X = any out of 20 proteinogenic amino acids

<220>
<221>  MISC_FEATURE
<222>  (10)..(10)
<223>  X = any out of 20 proteinogenic amino acids

<400>  8

Thr Val Xaa Thr Ser Arg Xaa Leu Ser Xaa Tyr Lys Leu 
1               5                   10              


<210>  9
<211>  30
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  2019-nCoV Nucleocapsid protein fragment

<400>  9

Ser Ala Ser Ala Phe Phe Gly Met Ser Arg Ile Gly Met Glu Val Thr 
1               5                   10                  15      


Pro Ser Gly Thr Trp Leu Thr Tyr Thr Gly Ala Ile Lys Leu 
            20                  25                  30  


<210>  10
<211>  30
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CoVV5 VEL Vaccine


<220>
<221>  MISC_FEATURE
<222>  (3)..(3)
<223>  X = any out of 20 proteinogenic amino acids

<220>
<221>  MISC_FEATURE
<222>  (6)..(6)
<223>  X = any out of 20 proteinogenic amino acids

<220>
<221>  MISC_FEATURE
<222>  (11)..(11)
<223>  X = any out of 20 proteinogenic amino acids

<220>
<221>  misc_feature
<222>  (25)..(25)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (27)..(27)
<223>  Xaa can be any naturally occurring amino acid

<400>  10

Ser Ala Xaa Ala Phe Xaa Gly Met Ser Arg Xaa Gly Met Glu Val Thr 
1               5                   10                  15      


Pro Ser Gly Thr Trp Leu Thr Tyr Xaa Gly Xaa Ile Lys Leu 
            20                  25                  30  


<210>  11
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  2019-nCoV ORF1ab protein fragment

<400>  11

Tyr Thr Met Ala Asp Leu Val Tyr Ala Leu 
1               5                   10  


<210>  12
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CoVV6 VEL Vaccine


<220>
<221>  MISC_FEATURE
<222>  (3)..(3)
<223>  X = any out of 20 proteinogenic amino acids

<220>
<221>  MISC_FEATURE
<222>  (6)..(6)
<223>  X = any out of 20 proteinogenic amino acids

<220>
<221>  MISC_FEATURE
<222>  (8)..(8)
<223>  X = any out of 20 proteinogenic amino acids

<400>  12

Tyr Thr Xaa Ala Asp Xaa Val Xaa Ala Leu 
1               5                   10  


<210>  13
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  2019-nCoV ORF1ab protein fragment

<400>  13

Ser Met Met Gly Phe Lys Met Asn Tyr 
1               5                   


<210>  14
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CoVV7 VEL Vaccine


<220>
<221>  MISC_FEATURE
<222>  (3)..(3)
<223>  X = any out of 20 proteinogenic amino acids

<220>
<221>  MISC_FEATURE
<222>  (5)..(5)
<223>  X = any out of 20 proteinogenic amino acids

<220>
<221>  MISC_FEATURE
<222>  (7)..(7)
<223>  X = any out of 20 proteinogenic amino acids

<400>  14

Ser Met Xaa Gly Xaa Lys Xaa Asn Tyr 
1               5                   


<210>  15
<211>  14
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  2019-nCoV ORF1ab protein fragment

<400>  15

Phe Leu Met Ser Phe Thr Val Leu Cys Leu Thr Pro Val Tyr 
1               5                   10                  


<210>  16
<211>  14
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CoVV8 VEL Vaccine


<220>
<221>  MISC_FEATURE
<222>  (4)..(4)
<223>  X = any out of 20 proteinogenic amino acids

<220>
<221>  MISC_FEATURE
<222>  (6)..(6)
<223>  X = any out of 20 proteinogenic amino acids

<220>
<221>  MISC_FEATURE
<222>  (10)..(10)
<223>  X = any out of 20 proteinogenic amino acids

<400>  16

Phe Leu Met Xaa Phe Xaa Val Leu Cys Xaa Thr Pro Val Tyr 
1               5                   10                  


<210>  17
<211>  40
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  2019-nCoV spike protein fragment

<400>  17

Lys Leu Asn Asp Leu Cys Phe Thr Asn Val Tyr Ala Asp Ser Phe Val 
1               5                   10                  15      


Ile Arg Gly Asp Glu Val Arg Gln Ile Ala Pro Gly Gln Thr Gly Lys 
            20                  25                  30          


Ile Ala Asp Tyr Asn Tyr Lys Leu 
        35                  40  


<210>  18
<211>  40
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CoVV9 VEL Vaccine


<220>
<221>  MISC_FEATURE
<222>  (6)..(6)
<223>  X = any out of 20 proteinogenic amino acids

<220>
<221>  MISC_FEATURE
<222>  (8)..(8)
<223>  X = any out of 20 proteinogenic amino acids

<220>
<221>  MISC_FEATURE
<222>  (22)..(22)
<223>  X = any out of 20 proteinogenic amino acids

<220>
<221>  MISC_FEATURE
<222>  (36)..(36)
<223>  X = any out of 20 proteinogenic amino acids

<220>
<221>  misc_feature
<222>  (38)..(38)
<223>  Xaa can be any naturally occurring amino acid

<400>  18

Lys Leu Asn Asp Leu Xaa Phe Xaa Asn Val Tyr Ala Asp Ser Phe Val 
1               5                   10                  15      


Ile Arg Gly Asp Glu Xaa Arg Gln Ile Ala Pro Gly Gln Thr Gly Lys 
            20                  25                  30          


Ile Ala Asp Xaa Asn Xaa Lys Leu 
        35                  40  


<210>  19
<211>  14
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  2019-nCoV spike protein fragment

<400>  19

Tyr Ile Trp Leu Gly Phe Ile Ala Gly Leu Ile Ala Ile Val 
1               5                   10                  


<210>  20
<211>  14
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CoVV10 VEL Vaccine


<220>
<221>  MISC_FEATURE
<222>  (5)..(5)
<223>  X = any out of 20 proteinogenic amino acids

<220>
<221>  MISC_FEATURE
<222>  (8)..(8)
<223>  X = any out of 20 proteinogenic amino acids

<220>
<221>  MISC_FEATURE
<222>  (10)..(10)
<223>  X = any out of 20 proteinogenic amino acids

<400>  20

Tyr Ile Trp Leu Xaa Phe Ile Xaa Gly Xaa Ile Ala Ile Val 
1               5                   10                  


<210>  21
<211>  20
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  2019-nCoV spike protein fragment

<400>  21

Cys Val Ala Asp Tyr Ser Val Leu Tyr Asn Ser Ala Ser Phe Ser Thr 
1               5                   10                  15      


Phe Lys Cys Tyr 
            20  


<210>  22
<211>  20
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CoVV11 VEL Vaccine


<220>
<221>  MISC_FEATURE
<222>  (5)..(5)
<223>  X = any out of 20 proteinogenic amino acids

<220>
<221>  MISC_FEATURE
<222>  (7)..(7)
<223>  X = any out of 20 proteinogenic amino acids

<220>
<221>  MISC_FEATURE
<222>  (17)..(17)
<223>  X = any out of 20 proteinogenic amino acids

<400>  22

Cys Val Ala Asp Xaa Ser Xaa Leu Tyr Asn Ser Ala Ser Phe Ser Thr 
1               5                   10                  15      


Xaa Lys Cys Tyr 
            20  


<210>  23
<211>  31
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  2019-nCoV spike protein fragment

<400>  23

Phe Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln Ala Gly Ser Thr Pro 
1               5                   10                  15      


Cys Asn Gly Val Glu Gly Phe Asn Cys Tyr Phe Pro Leu Gln Ser 
            20                  25                  30      


<210>  24
<211>  31
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  COV12  VEL Vaccine


<220>
<221>  MISC_FEATURE
<222>  (9)..(9)
<223>  X = any out of 20 proteinogenic amino acids

<220>
<221>  MISC_FEATURE
<222>  (12)..(12)
<223>  X = any out of 20 proteinogenic amino acids

<220>
<221>  MISC_FEATURE
<222>  (14)..(15)
<223>  X = any out of 20 proteinogenic amino acids

<220>
<221>  MISC_FEATURE
<222>  (20)..(20)
<223>  X = any out of 20 proteinogenic amino acids

<220>
<221>  MISC_FEATURE
<222>  (22)..(22)
<223>  X = any out of 20 proteinogenic amino acids

<400>  24

Phe Glu Arg Asp Ile Ser Thr Glu Xaa Tyr Gln Xaa Gly Xaa Thr Pro 
1               5                   10                  15      


Cys Asn Gly Xaa Glu Xaa Phe Asn Cys Tyr Phe Pro Leu Gln Ser 
            20                  25                  30      


