                         SEQUENCE LISTING

<110>  Montana State University
 
<120>  Engineered CRISPR-Cas Systems and Methods for Sensitive and 
       Specific Diagnostics

<130>  1127.006US1

<160>  246   

<170>  PatentIn version 3.5

<210>  1
<211>  76
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Artificial sequences generated for Engineered CRISPR-Cas Systems 
       and Methods for Sensitive and Specific Diagnostics

<400>  1
auugcgacac gcugaagcgc ugggggcaaa uugugcaauu ugcggccagu ugcaagggau       60

ugagccccgu aagggg                                                       76


<210>  2
<211>  56
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Artificial sequences generated for Engineered CRISPR-Cas Systems 
       and Methods for Sensitive and Specific Diagnostics

<400>  2
auugcgacac gcugaagcgc ugggggcaaa uugugcaauu ugcggccagu ugcaag           56


<210>  3
<211>  76
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Artificial sequences generated for Engineered CRISPR-Cas Systems 
       and Methods for Sensitive and Specific Diagnostics

<400>  3
auugcgacac gcugaagcgc ugggggcaaa uugugcaauu ugcggccagu ugcaagggau       60

ugagccccgu aagggg                                                       76


<210>  4
<211>  76
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Artificial sequences generated for Engineered CRISPR-Cas Systems 
       and Methods for Sensitive and Specific Diagnostics

<400>  4
auugcgacgg ccgacguugu uuugaucgcg ccccacugcg uucuccaugu ugcaagggau       60

ugagccccgu aagggg                                                       76


<210>  5
<211>  76
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Artificial sequences generated for Engineered CRISPR-Cas Systems 
       and Methods for Sensitive and Specific Diagnostics

<400>  5
auugcgacgu ugcgacuacg ugaugaggaa cgagaagagg cuugacuggu ugcaagggau       60

ugagccccgu aagggg                                                       76


<210>  6
<211>  76
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Artificial sequences generated for Engineered CRISPR-Cas Systems 
       and Methods for Sensitive and Specific Diagnostics

<400>  6
auugcgacag cagcagcaaa gcaagagcag caucaccgcc auugccaggu ugcaagggau       60

ugagccccgu aagggg                                                       76


<210>  7
<211>  76
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Artificial sequences generated for Engineered CRISPR-Cas Systems 
       and Methods for Sensitive and Specific Diagnostics

<400>  7
auugcgacau gcuuuagugg caguacguuu uugccgaggc uucuuagagu ugcaagggau       60

ugagccccgu aagggg                                                       76


<210>  8
<211>  76
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Artificial sequences generated for Engineered CRISPR-Cas Systems 
       and Methods for Sensitive and Specific Diagnostics

<400>  8
auugcgacuu ccgaagaacg cugaagcgcu gggggcaaau ugugcaaugu ugcaagggau       60

ugagccccgu aagggg                                                       76


<210>  9
<211>  76
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Artificial sequences generated for Engineered CRISPR-Cas Systems 
       and Methods for Sensitive and Specific Diagnostics

<400>  9
auugcgacau ucagcaaaau gacuugaucu uugaaauuug gaucuuuggu ugcaagggau       60

ugagccccgu aagggg                                                       76


<210>  10
<211>  76
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Artificial sequences generated for Engineered CRISPR-Cas Systems 
       and Methods for Sensitive and Specific Diagnostics

<400>  10
auugcgacca guuugcuguu ucuucugucu cugcgguaag gcuugagugu ugcaagggau       60

ugagccccgu aagggg                                                       76


<210>  11
<211>  76
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Artificial sequences generated for Engineered CRISPR-Cas Systems 
       and Methods for Sensitive and Specific Diagnostics

<400>  11
auugcgacgu cagcacugcu cauggauugu ugcaauuguu uggagaaagu ugcaagggau       60

ugagccccgu aagggg                                                       76


<210>  12
<211>  76
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Artificial sequences generated for Engineered CRISPR-Cas Systems 
       and Methods for Sensitive and Specific Diagnostics

<400>  12
auugcgacaa aagcgaaaac guuuauauag cccaucugcc uugugugggu ugcaagggau       60

ugagccccgu aagggg                                                       76


<210>  13
<211>  58
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificial sequences generated for Engineered CRISPR-Cas Systems 
       and Methods for Sensitive and Specific Diagnostics

<400>  13
gataatacga ctcactatag ggaactgatt acaaacattg gccgcaaatt gcacaatt         58


<210>  14
<211>  56
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificial sequences generated for Engineered CRISPR-Cas Systems 
       and Methods for Sensitive and Specific Diagnostics

<400>  14
gcgcgacatt ccgaagaacg ctgaagcgct gggggcaaat tgtgcaattt gcggcc           56


<210>  15
<211>  58
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificial sequences generated for Engineered CRISPR-Cas Systems 
       and Methods for Sensitive and Specific Diagnostics

<400>  15
gataatacga ctcactatag ggaactgatt acaaacattg gccgcaaatt gcacaatt         58


<210>  16
<211>  56
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificial sequences generated for Engineered CRISPR-Cas Systems 
       and Methods for Sensitive and Specific Diagnostics

<400>  16
gcgtgacatt ccaaagaatg cagaggcact tggagcaaat tgtgcaattt gcggcc           56


<210>  17
<211>  58
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificial sequences generated for Engineered CRISPR-Cas Systems 
       and Methods for Sensitive and Specific Diagnostics

<400>  17
ctagagctcg ataatacgac tcactatagg gcgtgttgtt ttagatttca tctaaacg         58


<210>  18
<211>  35
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificial sequences generated for Engineered CRISPR-Cas Systems 
       and Methods for Sensitive and Specific Diagnostics

<400>  18
atcctgcagg cacactgatt aaagattgct atgtg                                  35


<210>  19
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificial sequences generated for Engineered CRISPR-Cas Systems 
       and Methods for Sensitive and Specific Diagnostics

<400>  19
gctgctgagg cttctaag                                                     18


<210>  20
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificial sequences generated for Engineered CRISPR-Cas Systems 
       and Methods for Sensitive and Specific Diagnostics

<400>  20
gcgtcaatat gcttattcag c                                                 21


<210>  21
<211>  39
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificial sequences generated for Engineered CRISPR-Cas Systems 
       and Methods for Sensitive and Specific Diagnostics

<400>  21
tcagcgttct tcggaatgtc gctgtgtagg tcaaccacg                              39


<210>  22
<211>  40
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificial sequences generated for Engineered CRISPR-Cas Systems 
       and Methods for Sensitive and Specific Diagnostics

<400>  22
gcggccaatg tttgtaatca gtagacgtgg tccagaacaa                             40


<210>  23
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificial sequences generated for Engineered CRISPR-Cas Systems 
       and Methods for Sensitive and Specific Diagnostics

<400>  23
ccttgtctga ttagttcctg gt                                                22


<210>  24
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificial sequences generated for Engineered CRISPR-Cas Systems 
       and Methods for Sensitive and Specific Diagnostics

<400>  24
tggcatggaa gtcacacc                                                     18


<210>  25
<211>  38
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificial sequences generated for Engineered CRISPR-Cas Systems 
       and Methods for Sensitive and Specific Diagnostics

<400>  25
taatacgact cactataggg agacgtggtc cagaacaa                               38


<210>  26
<211>  140
<212>  RNA
<213>  Thermus thermophilus


<220>
<221>  misc_feature
<222>  (24)..(24)
<223>  n is a, c, g, or u

<220>
<221>  misc_feature
<222>  (28)..(28)
<223>  n is a, c, g, or u

<220>
<221>  misc_feature
<222>  (61)..(61)
<223>  n is a, c, g, or u

<220>
<221>  misc_feature
<222>  (93)..(93)
<223>  n is a, c, g, or u

<220>
<221>  misc_feature
<222>  (95)..(95)
<223>  n is a, c, g, or u

<400>  26
mkkkvrrsva kgrgmsrdma gddnvvrndy gsskgkryws ggdykakrvy asdkdvarga       60

ndrsavarrg rvrdaydaka rsarggykvr ggnanrrvag arrvmyrvdd dygkyradgg      120

ghsrgygvyh rdgwkrkvvv                                                  140


<210>  27
<211>  140
<212>  RNA
<213>  Thermus thermophilus


<220>
<221>  misc_feature
<222>  (24)..(24)
<223>  n is a, c, g, or u

<220>
<221>  misc_feature
<222>  (28)..(28)
<223>  n is a, c, g, or u

<220>
<221>  misc_feature
<222>  (61)..(61)
<223>  n is a, c, g, or u

<220>
<221>  misc_feature
<222>  (93)..(93)
<223>  n is a, c, g, or u

<220>
<221>  misc_feature
<222>  (95)..(95)
<223>  n is a, c, g, or u

<400>  27
mkkkvrrsva kgrgmsrdma gdanvvrndy gsskgkryws ggdykakrvy asdkdvarga       60

ndrsavarrg rvrdaydaka rsarggykvr ggnanrrvag arrvmyrvdd dygkyradgg      120

ghsrgygvyh rdgwkrkvvv                                                  140


<210>  28
<211>  37
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificial sequences generated for Engineered CRISPR-Cas Systems 
       and Methods for Sensitive and Specific Diagnostics

<400>  28
taatacgact cactataggg agacgtgtcc agaacaa                                37


<210>  29
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificial sequences generated for Engineered CRISPR-Cas Systems 
       and Methods for Sensitive and Specific Diagnostics

<400>  29
accaaccaac tttcgatctc ttgt                                              24


<210>  30
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificial sequences generated for Engineered CRISPR-Cas Systems 
       and Methods for Sensitive and Specific Diagnostics

<400>  30
catctttaag atgttgacgt gcctc                                             25


<210>  31
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificial sequences generated for Engineered CRISPR-Cas Systems 
       and Methods for Sensitive and Specific Diagnostics

<400>  31
cggtaataaa ggagctggtg gc                                                22


<210>  32
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificial sequences generated for Engineered CRISPR-Cas Systems 
       and Methods for Sensitive and Specific Diagnostics

<400>  32
aaggtgtctg caattcatag ctct                                              24


<210>  33
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificial sequences generated for Engineered CRISPR-Cas Systems 
       and Methods for Sensitive and Specific Diagnostics

<400>  33
tggtgaaact tcatggcaga cg                                                22


<210>  34
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificial sequences generated for Engineered CRISPR-Cas Systems 
       and Methods for Sensitive and Specific Diagnostics

<400>  34
attgatgttg actttctctt tttggagt                                          28


<210>  35
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificial sequences generated for Engineered CRISPR-Cas Systems 
       and Methods for Sensitive and Specific Diagnostics

<400>  35
atcagaggct gctcgtgttg ta                                                22


<210>  36
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificial sequences generated for Engineered CRISPR-Cas Systems 
       and Methods for Sensitive and Specific Diagnostics

<400>  36
catttgcatc agaggctgct cg                                                22


<210>  37
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificial sequences generated for Engineered CRISPR-Cas Systems 
       and Methods for Sensitive and Specific Diagnostics

<400>  37
tgcacaggtg acaatttgtc ca                                                22


<210>  38
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificial sequences generated for Engineered CRISPR-Cas Systems 
       and Methods for Sensitive and Specific Diagnostics

<400>  38
aggtgacaat ttgtccaccg ac                                                22


<210>  39
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificial sequences generated for Engineered CRISPR-Cas Systems 
       and Methods for Sensitive and Specific Diagnostics

<400>  39
tcccacagaa gtgttaacag agga                                              24


<210>  40
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificial sequences generated for Engineered CRISPR-Cas Systems 
       and Methods for Sensitive and Specific Diagnostics

<400>  40
ttcccacaga agtgttaaca gagg                                              24


<210>  41
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificial sequences generated for Engineered CRISPR-Cas Systems 
       and Methods for Sensitive and Specific Diagnostics

<400>  41
atgacagcat ctgccacaac ac                                                22


<210>  42
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificial sequences generated for Engineered CRISPR-Cas Systems 
       and Methods for Sensitive and Specific Diagnostics

<400>  42
gacagcatct gccacaacac ag                                                22


<210>  43
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificial sequences generated for Engineered CRISPR-Cas Systems 
       and Methods for Sensitive and Specific Diagnostics

<400>  43
ggaatttggt gccacttctg ct                                                22


<210>  44
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificial sequences generated for Engineered CRISPR-Cas Systems 
       and Methods for Sensitive and Specific Diagnostics

<400>  44
tcatcagatt caacttgcat ggca                                              24


<210>  45
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificial sequences generated for Engineered CRISPR-Cas Systems 
       and Methods for Sensitive and Specific Diagnostics

<400>  45
tcgcacaaat gtctacttag ctgt                                              24


<210>  46
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificial sequences generated for Engineered CRISPR-Cas Systems 
       and Methods for Sensitive and Specific Diagnostics

<400>  46
accacagcag ttaaaacacc ct                                                22


<210>  47
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificial sequences generated for Engineered CRISPR-Cas Systems 
       and Methods for Sensitive and Specific Diagnostics

<400>  47
acagtgctta aaaagtgtaa aagtgcc                                           27


<210>  48
<211>  26
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificial sequences generated for Engineered CRISPR-Cas Systems 
       and Methods for Sensitive and Specific Diagnostics

<400>  48
agtgcttaaa aagtgtaaaa gtgcct                                            26


<210>  49
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificial sequences generated for Engineered CRISPR-Cas Systems 
       and Methods for Sensitive and Specific Diagnostics

<400>  49
aacagaaact gtagctggca ct                                                22


<210>  50
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificial sequences generated for Engineered CRISPR-Cas Systems 
       and Methods for Sensitive and Specific Diagnostics

<400>  50
actgtagctg gcactttgag aga                                               23


<210>  51
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificial sequences generated for Engineered CRISPR-Cas Systems 
       and Methods for Sensitive and Specific Diagnostics

<400>  51
cttctttctt tgagagaagt gaggact                                           27


<210>  52
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificial sequences generated for Engineered CRISPR-Cas Systems 
       and Methods for Sensitive and Specific Diagnostics

<400>  52
tttgttggag tgttaacaat gcagt                                             25


<210>  53
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificial sequences generated for Engineered CRISPR-Cas Systems 
       and Methods for Sensitive and Specific Diagnostics

<400>  53
gctgttatgt acatgggcac act                                               23


<210>  54
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificial sequences generated for Engineered CRISPR-Cas Systems 
       and Methods for Sensitive and Specific Diagnostics

<400>  54
tgtccaactt agggtcaatt tctgt                                             25


<210>  55
<211>  29
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificial sequences generated for Engineered CRISPR-Cas Systems 
       and Methods for Sensitive and Specific Diagnostics

<400>  55
tggctattga ttataaacac tacacaccc                                         29


<210>  56
<211>  29
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificial sequences generated for Engineered CRISPR-Cas Systems 
       and Methods for Sensitive and Specific Diagnostics

<400>  56
ggctattgat tataaacact acacaccct                                         29


<210>  57
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificial sequences generated for Engineered CRISPR-Cas Systems 
       and Methods for Sensitive and Specific Diagnostics

<400>  57
tagatctgtg tggccaacct ct                                                22


<210>  58
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificial sequences generated for Engineered CRISPR-Cas Systems 
       and Methods for Sensitive and Specific Diagnostics

<400>  58
gatctgtgtg gccaacctct tc                                                22


<210>  59
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificial sequences generated for Engineered CRISPR-Cas Systems 
       and Methods for Sensitive and Specific Diagnostics

<400>  59
acaactacta acatagttac acggtgt                                           27


<210>  60
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificial sequences generated for Engineered CRISPR-Cas Systems 
       and Methods for Sensitive and Specific Diagnostics

<400>  60
accagtacag taggttgcaa tagtg                                             25


<210>  61
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificial sequences generated for Engineered CRISPR-Cas Systems 
       and Methods for Sensitive and Specific Diagnostics

<400>  61
gcaattgttt ttcagctatt ttgcagt                                           27


<210>  62
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificial sequences generated for Engineered CRISPR-Cas Systems 
       and Methods for Sensitive and Specific Diagnostics

<400>  62
actgtagtga caagtctctc gca                                               23


<210>  63
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificial sequences generated for Engineered CRISPR-Cas Systems 
       and Methods for Sensitive and Specific Diagnostics

<400>  63
actacagtca gcttatgtgt caacc                                             25


<210>  64
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificial sequences generated for Engineered CRISPR-Cas Systems 
       and Methods for Sensitive and Specific Diagnostics

<400>  64
aatacaagca ccaaggtcac gg                                                22


<210>  65
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificial sequences generated for Engineered CRISPR-Cas Systems 
       and Methods for Sensitive and Specific Diagnostics

<400>  65
acttgtgttc ctttttgttg ctgc                                              24


<210>  66
<211>  29
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificial sequences generated for Engineered CRISPR-Cas Systems 
       and Methods for Sensitive and Specific Diagnostics

<400>  66
agtgtactct ataagttttg atggtgtgt                                         29


<210>  67
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificial sequences generated for Engineered CRISPR-Cas Systems 
       and Methods for Sensitive and Specific Diagnostics

<400>  67
ttctgagtac tgtaggcacg gc                                                22


<210>  68
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificial sequences generated for Engineered CRISPR-Cas Systems 
       and Methods for Sensitive and Specific Diagnostics

<400>  68
acagaataaa caccaggtaa gaatgagt                                          28


<210>  69
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificial sequences generated for Engineered CRISPR-Cas Systems 
       and Methods for Sensitive and Specific Diagnostics

<400>  69
acttttgaag aagctgcgct gt                                                22


<210>  70
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificial sequences generated for Engineered CRISPR-Cas Systems 
       and Methods for Sensitive and Specific Diagnostics

<400>  70
tggacagtaa actacgtcat caagc                                             25


<210>  71
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificial sequences generated for Engineered CRISPR-Cas Systems 
       and Methods for Sensitive and Specific Diagnostics

<400>  71
tgttcgcatt caaccaggac ag                                                22


<210>  72
<211>  26
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificial sequences generated for Engineered CRISPR-Cas Systems 
       and Methods for Sensitive and Specific Diagnostics

<400>  72
acttcatagc cacaaggtta aagtca                                            26


<210>  73
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificial sequences generated for Engineered CRISPR-Cas Systems 
       and Methods for Sensitive and Specific Diagnostics

<400>  73
acacaccact ggttgttact cac                                               23


<210>  74
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificial sequences generated for Engineered CRISPR-Cas Systems 
       and Methods for Sensitive and Specific Diagnostics

<400>  74
gtccacactc tcctagcacc at                                                22


<210>  75
<211>  29
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificial sequences generated for Engineered CRISPR-Cas Systems 
       and Methods for Sensitive and Specific Diagnostics

<400>  75
agtattgccc tattttcttc ataactggt                                         29


<210>  76
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificial sequences generated for Engineered CRISPR-Cas Systems 
       and Methods for Sensitive and Specific Diagnostics

<400>  76
tgtaactgga cacattgagc cc                                                22


<210>  77
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificial sequences generated for Engineered CRISPR-Cas Systems 
       and Methods for Sensitive and Specific Diagnostics

<400>  77
gttcccttcc atcatatgca gct                                               23


<210>  78
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificial sequences generated for Engineered CRISPR-Cas Systems 
       and Methods for Sensitive and Specific Diagnostics

<400>  78
tggtatgaca accattagtt tggct                                             25


<210>  79
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificial sequences generated for Engineered CRISPR-Cas Systems 
       and Methods for Sensitive and Specific Diagnostics

<400>  79
tacgacagat gtcttgtgct gc                                                22


<210>  80
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificial sequences generated for Engineered CRISPR-Cas Systems 
       and Methods for Sensitive and Specific Diagnostics

<400>  80
agcagcatct acagcaaaag ca                                                22


<210>  81
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificial sequences generated for Engineered CRISPR-Cas Systems 
       and Methods for Sensitive and Specific Diagnostics

<400>  81
tacctacaac ttgtgctaat gaccc                                             25


<210>  82
<211>  29
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificial sequences generated for Engineered CRISPR-Cas Systems 
       and Methods for Sensitive and Specific Diagnostics

<400>  82
agtatgtaca aatacctaca acttgtgct                                         29


<210>  83
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificial sequences generated for Engineered CRISPR-Cas Systems 
       and Methods for Sensitive and Specific Diagnostics

<400>  83
aaattgtttc ttcatgttgg tagttagaga                                        30


<210>  84
<211>  29
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificial sequences generated for Engineered CRISPR-Cas Systems 
       and Methods for Sensitive and Specific Diagnostics

<400>  84
ttcatgttgg tagttagaga aagtgtgtc                                         29


<210>  85
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificial sequences generated for Engineered CRISPR-Cas Systems 
       and Methods for Sensitive and Specific Diagnostics

<400>  85
aggactggta tgattttgta gaaaaccc                                          28


<210>  86
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificial sequences generated for Engineered CRISPR-Cas Systems 
       and Methods for Sensitive and Specific Diagnostics

<400>  86
aataacggtc aaagagtttt aacctctc                                          28


<210>  87
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificial sequences generated for Engineered CRISPR-Cas Systems 
       and Methods for Sensitive and Specific Diagnostics

<400>  87
aggaattact tgtgtatgct gctga                                             25


<210>  88
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificial sequences generated for Engineered CRISPR-Cas Systems 
       and Methods for Sensitive and Specific Diagnostics

<400>  88
tgacgatgac ttggttagca ttaataca                                          28


<210>  89
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificial sequences generated for Engineered CRISPR-Cas Systems 
       and Methods for Sensitive and Specific Diagnostics

<400>  89
tcaatagccg ccactagagg ag                                                22


<210>  90
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificial sequences generated for Engineered CRISPR-Cas Systems 
       and Methods for Sensitive and Specific Diagnostics

<400>  90
agtgcattaa cattggccgt ga                                                22


<210>  91
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificial sequences generated for Engineered CRISPR-Cas Systems 
       and Methods for Sensitive and Specific Diagnostics

<400>  91
agcaaaatgt tggactgaga ctga                                              24


<210>  92
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificial sequences generated for Engineered CRISPR-Cas Systems 
       and Methods for Sensitive and Specific Diagnostics

<400>  92
agcctcataa aactcaggtt ccc                                               23


<210>  93
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificial sequences generated for Engineered CRISPR-Cas Systems 
       and Methods for Sensitive and Specific Diagnostics

<400>  93
actcaacttt acttaggagg tatgagct                                          28


<210>  94
<211>  29
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificial sequences generated for Engineered CRISPR-Cas Systems 
       and Methods for Sensitive and Specific Diagnostics

<400>  94
ggtgtactct cctatttgta ctttactgt                                         29


<210>  95
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificial sequences generated for Engineered CRISPR-Cas Systems 
       and Methods for Sensitive and Specific Diagnostics

<400>  95
attctacact ccagggacca cc                                                22


<210>  96
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificial sequences generated for Engineered CRISPR-Cas Systems 
       and Methods for Sensitive and Specific Diagnostics

<400>  96
gtaattgagc agggtcgcca at                                                22


<210>  97
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificial sequences generated for Engineered CRISPR-Cas Systems 
       and Methods for Sensitive and Specific Diagnostics

<400>  97
tcacgcatga tgtttcatct gca                                               23


<210>  98
<211>  26
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificial sequences generated for Engineered CRISPR-Cas Systems 
       and Methods for Sensitive and Specific Diagnostics

<400>  98
aagagtcctg ttacattttc agcttg                                            26


<210>  99
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificial sequences generated for Engineered CRISPR-Cas Systems 
       and Methods for Sensitive and Specific Diagnostics

<400>  99
tgtttatcac ccgcgaagaa gc                                                22


<210>  100
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificial sequences generated for Engineered CRISPR-Cas Systems 
       and Methods for Sensitive and Specific Diagnostics

<400>  100
atcacataga caacaggtgc gc                                                22


<210>  101
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificial sequences generated for Engineered CRISPR-Cas Systems 
       and Methods for Sensitive and Specific Diagnostics

<400>  101
tgttaagcgt gttgactgga ct                                                22


<210>  102
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificial sequences generated for Engineered CRISPR-Cas Systems 
       and Methods for Sensitive and Specific Diagnostics

<400>  102
acaaactgcc accatcacaa cc                                                22


<210>  103
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificial sequences generated for Engineered CRISPR-Cas Systems 
       and Methods for Sensitive and Specific Diagnostics

<400>  103
gctggcttta gcttgtgggt tt                                                22


<210>  104
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificial sequences generated for Engineered CRISPR-Cas Systems 
       and Methods for Sensitive and Specific Diagnostics

<400>  104
tgtcagtcat agaacaaaca ccaatagt                                          28


<210>  105
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificial sequences generated for Engineered CRISPR-Cas Systems 
       and Methods for Sensitive and Specific Diagnostics

<400>  105
gttgtccaac aattacctga aacttact                                          28


<210>  106
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificial sequences generated for Engineered CRISPR-Cas Systems 
       and Methods for Sensitive and Specific Diagnostics

<400>  106
caaccttaga aactacagat aaatcttggg                                        30


<210>  107
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificial sequences generated for Engineered CRISPR-Cas Systems 
       and Methods for Sensitive and Specific Diagnostics

<400>  107
tgtcgcaaaa tatactcaac tgtgtca                                           27


<210>  108
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificial sequences generated for Engineered CRISPR-Cas Systems 
       and Methods for Sensitive and Specific Diagnostics

<400>  108
tctttatagc cacggaacct cca                                               23


<210>  109
<211>  29
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificial sequences generated for Engineered CRISPR-Cas Systems 
       and Methods for Sensitive and Specific Diagnostics

<400>  109
acaaatccaa ttcagttgtc ttcctattc                                         29


<210>  110
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificial sequences generated for Engineered CRISPR-Cas Systems 
       and Methods for Sensitive and Specific Diagnostics

<400>  110
tggaaaagaa aggtaagaac aagtcct                                           27


<210>  111
<211>  29
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificial sequences generated for Engineered CRISPR-Cas Systems 
       and Methods for Sensitive and Specific Diagnostics

<400>  111
caattttgta atgatccatt tttgggtgt                                         29


<210>  112
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificial sequences generated for Engineered CRISPR-Cas Systems 
       and Methods for Sensitive and Specific Diagnostics

<400>  112
caccagctgt ccaacctgaa ga                                                22


<210>  113
<211>  26
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificial sequences generated for Engineered CRISPR-Cas Systems 
       and Methods for Sensitive and Specific Diagnostics

<400>  113
agagtccaac caacagaatc tattgt                                            26


<210>  114
<211>  26
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificial sequences generated for Engineered CRISPR-Cas Systems 
       and Methods for Sensitive and Specific Diagnostics

<400>  114
accaccaacc ttagaatcaa gattgt                                            26


<210>  115
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificial sequences generated for Engineered CRISPR-Cas Systems 
       and Methods for Sensitive and Specific Diagnostics

<400>  115
ccagcaactg tttgtggacc ta                                                22


<210>  116
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificial sequences generated for Engineered CRISPR-Cas Systems 
       and Methods for Sensitive and Specific Diagnostics

<400>  116
cagcccctat taaacagcct gc                                                22


<210>  117
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificial sequences generated for Engineered CRISPR-Cas Systems 
       and Methods for Sensitive and Specific Diagnostics

<400>  117
gtggtgattc aactgaatgc agc                                               23


<210>  118
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificial sequences generated for Engineered CRISPR-Cas Systems 
       and Methods for Sensitive and Specific Diagnostics

<400>  118
catttcatct gtgagcaaag gtgg                                              24


<210>  119
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificial sequences generated for Engineered CRISPR-Cas Systems 
       and Methods for Sensitive and Specific Diagnostics

<400>  119
gcacttggaa aacttcaaga tgtgg                                             25


<210>  120
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificial sequences generated for Engineered CRISPR-Cas Systems 
       and Methods for Sensitive and Specific Diagnostics

<400>  120
gtgaagttct tttcttgtgc aggg                                              24


<210>  121
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificial sequences generated for Engineered CRISPR-Cas Systems 
       and Methods for Sensitive and Specific Diagnostics

<400>  121
tcctttgcaa cctgaattag actca                                             25


<210>  122
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificial sequences generated for Engineered CRISPR-Cas Systems 
       and Methods for Sensitive and Specific Diagnostics

<400>  122
tttgactcct ttgagcactg gc                                                22


<210>  123
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificial sequences generated for Engineered CRISPR-Cas Systems 
       and Methods for Sensitive and Specific Diagnostics

<400>  123
actagcactc tccaagggtg tt                                                22


<210>  124
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificial sequences generated for Engineered CRISPR-Cas Systems 
       and Methods for Sensitive and Specific Diagnostics

<400>  124
acacagtctt ttactccaga ttccc                                             25


<210>  125
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificial sequences generated for Engineered CRISPR-Cas Systems 
       and Methods for Sensitive and Specific Diagnostics

<400>  125
cgactactag cgtgcctttg ta                                                22


<210>  126
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificial sequences generated for Engineered CRISPR-Cas Systems 
       and Methods for Sensitive and Specific Diagnostics

<400>  126
actaggttcc attgttcaag gagc                                              24


<210>  127
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificial sequences generated for Engineered CRISPR-Cas Systems 
       and Methods for Sensitive and Specific Diagnostics

<400>  127
gtacgcgttc catgtggtca tt                                                22


<210>  128
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificial sequences generated for Engineered CRISPR-Cas Systems 
       and Methods for Sensitive and Specific Diagnostics

<400>  128
cgcgttccat gtggtcattc aa                                                22


<210>  129
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificial sequences generated for Engineered CRISPR-Cas Systems 
       and Methods for Sensitive and Specific Diagnostics

<400>  129
acctgaaagt caacgagatg aaaca                                             25


<210>  130
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificial sequences generated for Engineered CRISPR-Cas Systems 
       and Methods for Sensitive and Specific Diagnostics

<400>  130
acgagatgaa acatctgttg tcact                                             25


<210>  131
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificial sequences generated for Engineered CRISPR-Cas Systems 
       and Methods for Sensitive and Specific Diagnostics

<400>  131
tcactaccaa gagtgtgtta gaggt                                             25


<210>  132
<211>  29
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificial sequences generated for Engineered CRISPR-Cas Systems 
       and Methods for Sensitive and Specific Diagnostics

<400>  132
ttcaagtgag aaccaaaaga taataagca                                         29


<210>  133
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificial sequences generated for Engineered CRISPR-Cas Systems 
       and Methods for Sensitive and Specific Diagnostics

<400>  133
tgaggctggt tctaaatcac cca                                               23


<210>  134
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificial sequences generated for Engineered CRISPR-Cas Systems 
       and Methods for Sensitive and Specific Diagnostics

<400>  134
aggtcttcct tgccatgttg ag                                                22


<210>  135
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificial sequences generated for Engineered CRISPR-Cas Systems 
       and Methods for Sensitive and Specific Diagnostics

<400>  135
tgagggagcc ttgaatacac ca                                                22


<210>  136
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificial sequences generated for Engineered CRISPR-Cas Systems 
       and Methods for Sensitive and Specific Diagnostics

<400>  136
cagtacgttt ttgccgaggc tt                                                22


<210>  137
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificial sequences generated for Engineered CRISPR-Cas Systems 
       and Methods for Sensitive and Specific Diagnostics

<400>  137
tggatgacaa agatccaaat ttcaaaga                                          28


<210>  138
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificial sequences generated for Engineered CRISPR-Cas Systems 
       and Methods for Sensitive and Specific Diagnostics

<400>  138
acacactgat taaagattgc tatgtgag                                          28


<210>  139
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificial sequences generated for Engineered CRISPR-Cas Systems 
       and Methods for Sensitive and Specific Diagnostics

<400>  139
ctgttttaca ggttcgcgac gt                                                22


<210>  140
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificial sequences generated for Engineered CRISPR-Cas Systems 
       and Methods for Sensitive and Specific Diagnostics

<400>  140
taaggatcag tgccaagctc gt                                                22


<210>  141
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificial sequences generated for Engineered CRISPR-Cas Systems 
       and Methods for Sensitive and Specific Diagnostics

<400>  141
ggtgtatact gctgccgtga ac                                                22


<210>  142
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificial sequences generated for Engineered CRISPR-Cas Systems 
       and Methods for Sensitive and Specific Diagnostics

<400>  142
cacaagtagt ggcaccttct ttagt                                             25


<210>  143
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificial sequences generated for Engineered CRISPR-Cas Systems 
       and Methods for Sensitive and Specific Diagnostics

<400>  143
ggtgttgttg gagaaggttc cg                                                22


<210>  144
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificial sequences generated for Engineered CRISPR-Cas Systems 
       and Methods for Sensitive and Specific Diagnostics

<400>  144
tagcggcctt ctgtaaaaca cg                                                22


<210>  145
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificial sequences generated for Engineered CRISPR-Cas Systems 
       and Methods for Sensitive and Specific Diagnostics

<400>  145
agagtttctt agagacggtt ggga                                              24


<210>  146
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificial sequences generated for Engineered CRISPR-Cas Systems 
       and Methods for Sensitive and Specific Diagnostics

<400>  146
gcttcaacag cttcactagt aggt                                              24


<210>  147
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificial sequences generated for Engineered CRISPR-Cas Systems 
       and Methods for Sensitive and Specific Diagnostics

<400>  147
tgagaagtgc tctgcctata cagt                                              24


<210>  148
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificial sequences generated for Engineered CRISPR-Cas Systems 
       and Methods for Sensitive and Specific Diagnostics

<400>  148
tcatctaacc aatcttcttc ttgctct                                           27


<210>  149
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificial sequences generated for Engineered CRISPR-Cas Systems 
       and Methods for Sensitive and Specific Diagnostics

<400>  149
aaacatggag gaggtgttgc ag                                                22


<210>  150
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificial sequences generated for Engineered CRISPR-Cas Systems 
       and Methods for Sensitive and Specific Diagnostics

<400>  150
ttcactcttc atttccaaaa agcttga                                           27


<210>  151
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificial sequences generated for Engineered CRISPR-Cas Systems 
       and Methods for Sensitive and Specific Diagnostics

<400>  151
catccagatt ctgccactct tgt                                               23


<210>  152
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificial sequences generated for Engineered CRISPR-Cas Systems 
       and Methods for Sensitive and Specific Diagnostics

<400>  152
tggcaatctt catccagatt ctgc                                              24


<210>  153
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificial sequences generated for Engineered CRISPR-Cas Systems 
       and Methods for Sensitive and Specific Diagnostics

<400>  153
agtttccaca cagacaggca tt                                                22


<210>  154
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificial sequences generated for Engineered CRISPR-Cas Systems 
       and Methods for Sensitive and Specific Diagnostics

<400>  154
tgcgtgtttc ttctgcatgt gc                                                22


<210>  155
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificial sequences generated for Engineered CRISPR-Cas Systems 
       and Methods for Sensitive and Specific Diagnostics

<400>  155
aatttggaag aagctgctcg gt                                                22


<210>  156
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificial sequences generated for Engineered CRISPR-Cas Systems 
       and Methods for Sensitive and Specific Diagnostics

<400>  156
cacaacttgc gtgtggaggt ta                                                22


<210>  157
<211>  29
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificial sequences generated for Engineered CRISPR-Cas Systems 
       and Methods for Sensitive and Specific Diagnostics

<400>  157
tggaaatacc cacaagttaa tggtttaac                                         29


<210>  158
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificial sequences generated for Engineered CRISPR-Cas Systems 
       and Methods for Sensitive and Specific Diagnostics

<400>  158
acttctatta aatgggcaga taacaactgt                                        30


<210>  159
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificial sequences generated for Engineered CRISPR-Cas Systems 
       and Methods for Sensitive and Specific Diagnostics

<400>  159
agcttgttta ccacacgtac aagg                                              24


<210>  160
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificial sequences generated for Engineered CRISPR-Cas Systems 
       and Methods for Sensitive and Specific Diagnostics

<400>  160
gcttgtttac cacacgtaca agg                                               23


<210>  161
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificial sequences generated for Engineered CRISPR-Cas Systems 
       and Methods for Sensitive and Specific Diagnostics

<400>  161
acaaagaaaa cagttacaca acaacca                                           27


<210>  162
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificial sequences generated for Engineered CRISPR-Cas Systems 
       and Methods for Sensitive and Specific Diagnostics

<400>  162
acgtggcttt attagttgca ttgtt                                             25


<210>  163
<211>  29
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificial sequences generated for Engineered CRISPR-Cas Systems 
       and Methods for Sensitive and Specific Diagnostics

<400>  163
actaccgaag ttgtaggaga cattatact                                         29


<210>  164
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificial sequences generated for Engineered CRISPR-Cas Systems 
       and Methods for Sensitive and Specific Diagnostics

<400>  164
acagtattct ttgctatagt agtcggc                                           27


<210>  165
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificial sequences generated for Engineered CRISPR-Cas Systems 
       and Methods for Sensitive and Specific Diagnostics

<400>  165
aggcatgcct tcttactgta ctg                                               23


<210>  166
<211>  26
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificial sequences generated for Engineered CRISPR-Cas Systems 
       and Methods for Sensitive and Specific Diagnostics

<400>  166
acattctaac catagctgaa atcggg                                            26


<210>  167
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificial sequences generated for Engineered CRISPR-Cas Systems 
       and Methods for Sensitive and Specific Diagnostics

<400>  167
ttgtgataca ttctgtgctg gtagt                                             25


<210>  168
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificial sequences generated for Engineered CRISPR-Cas Systems 
       and Methods for Sensitive and Specific Diagnostics

<400>  168
tccgcactat caccaacatc ag                                                22


<210>  169
<211>  26
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificial sequences generated for Engineered CRISPR-Cas Systems 
       and Methods for Sensitive and Specific Diagnostics

<400>  169
acatagaagt tactggcgat agttgt                                            26


<210>  170
<211>  26
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificial sequences generated for Engineered CRISPR-Cas Systems 
       and Methods for Sensitive and Specific Diagnostics

<400>  170
tgtttagaca tgacatgaac aggtgt                                            26


<210>  171
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificial sequences generated for Engineered CRISPR-Cas Systems 
       and Methods for Sensitive and Specific Diagnostics

<400>  171
gcacaactaa tggtgacttt ttgca                                             25


<210>  172
<211>  26
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificial sequences generated for Engineered CRISPR-Cas Systems 
       and Methods for Sensitive and Specific Diagnostics

<400>  172
accactagta gatacacaaa caccag                                            26


<210>  173
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificial sequences generated for Engineered CRISPR-Cas Systems 
       and Methods for Sensitive and Specific Diagnostics

<400>  173
tggtgaatac agtcatgtag ttgcc                                             25


<210>  174
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificial sequences generated for Engineered CRISPR-Cas Systems 
       and Methods for Sensitive and Specific Diagnostics

<400>  174
agcacatcac tacgcaactt taga                                              24


<210>  175
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificial sequences generated for Engineered CRISPR-Cas Systems 
       and Methods for Sensitive and Specific Diagnostics

<400>  175
tcccatctgg taaagttgag ggt                                               23


<210>  176
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificial sequences generated for Engineered CRISPR-Cas Systems 
       and Methods for Sensitive and Specific Diagnostics

<400>  176
agtgaaattg ggcctcatag ca                                                22


<210>  177
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificial sequences generated for Engineered CRISPR-Cas Systems 
       and Methods for Sensitive and Specific Diagnostics

<400>  177
ttagcttggt tgtacgctgc tg                                                22


<210>  178
<211>  26
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificial sequences generated for Engineered CRISPR-Cas Systems 
       and Methods for Sensitive and Specific Diagnostics

<400>  178
gaacaaagac cattgagtac tctgga                                            26


<210>  179
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificial sequences generated for Engineered CRISPR-Cas Systems 
       and Methods for Sensitive and Specific Diagnostics

<400>  179
actgtgttat gtatgcatca gctgt                                             25


<210>  180
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificial sequences generated for Engineered CRISPR-Cas Systems 
       and Methods for Sensitive and Specific Diagnostics

<400>  180
caccaagagt cagtctaaag tagcg                                             25


<210>  181
<211>  26
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificial sequences generated for Engineered CRISPR-Cas Systems 
       and Methods for Sensitive and Specific Diagnostics

<400>  181
tgcacatcag tagtcttact ctcagt                                            26


<210>  182
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificial sequences generated for Engineered CRISPR-Cas Systems 
       and Methods for Sensitive and Specific Diagnostics

<400>  182
catggctgca tcacggtcaa at                                                22


<210>  183
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificial sequences generated for Engineered CRISPR-Cas Systems 
       and Methods for Sensitive and Specific Diagnostics

<400>  183
tgcaagagat ggttgtgttc cc                                                22


<210>  184
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificial sequences generated for Engineered CRISPR-Cas Systems 
       and Methods for Sensitive and Specific Diagnostics

<400>  184
cctacctccc tttgttgtgt tgt                                               23


<210>  185
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificial sequences generated for Engineered CRISPR-Cas Systems 
       and Methods for Sensitive and Specific Diagnostics

<400>  185
tgccacagta cgtctacaag ct                                                22


<210>  186
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificial sequences generated for Engineered CRISPR-Cas Systems 
       and Methods for Sensitive and Specific Diagnostics

<400>  186
ccacagtacg tctacaagct gg                                                22


<210>  187
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificial sequences generated for Engineered CRISPR-Cas Systems 
       and Methods for Sensitive and Specific Diagnostics

<400>  187
aacctttcca cataccgcag ac                                                22


<210>  188
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificial sequences generated for Engineered CRISPR-Cas Systems 
       and Methods for Sensitive and Specific Diagnostics

<400>  188
cgcagacggt acagactgtg tt                                                22


<210>  189
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificial sequences generated for Engineered CRISPR-Cas Systems 
       and Methods for Sensitive and Specific Diagnostics

<400>  189
tgtcgcttcc aagaaaagga cg                                                22


<210>  190
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificial sequences generated for Engineered CRISPR-Cas Systems 
       and Methods for Sensitive and Specific Diagnostics

<400>  190
cgcttccaag aaaaggacga aga                                               23


<210>  191
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificial sequences generated for Engineered CRISPR-Cas Systems 
       and Methods for Sensitive and Specific Diagnostics

<400>  191
cacgttcacc taagttggcg ta                                                22


<210>  192
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificial sequences generated for Engineered CRISPR-Cas Systems 
       and Methods for Sensitive and Specific Diagnostics

<400>  192
cacgttcacc taagttggcg tat                                               23


<210>  193
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificial sequences generated for Engineered CRISPR-Cas Systems 
       and Methods for Sensitive and Specific Diagnostics

<400>  193
tgttgacact gacttaacaa agcct                                             25


<210>  194
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificial sequences generated for Engineered CRISPR-Cas Systems 
       and Methods for Sensitive and Specific Diagnostics

<400>  194
tagattacca gaagcagcgt gc                                                22


<210>  195
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificial sequences generated for Engineered CRISPR-Cas Systems 
       and Methods for Sensitive and Specific Diagnostics

<400>  195
gttgataagt actttgattg ttacgatggt                                        30


<210>  196
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificial sequences generated for Engineered CRISPR-Cas Systems 
       and Methods for Sensitive and Specific Diagnostics

<400>  196
taacatgttg tgccaaccac ca                                                22


<210>  197
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificial sequences generated for Engineered CRISPR-Cas Systems 
       and Methods for Sensitive and Specific Diagnostics

<400>  197
catcaggaga tgccacaact gc                                                22


<210>  198
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificial sequences generated for Engineered CRISPR-Cas Systems 
       and Methods for Sensitive and Specific Diagnostics

<400>  198
gttgagagca aaattcatga ggtcc                                             25


<210>  199
<211>  26
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificial sequences generated for Engineered CRISPR-Cas Systems 
       and Methods for Sensitive and Specific Diagnostics

<400>  199
tgagttaaca ggacacatgt tagaca                                            26


<210>  200
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificial sequences generated for Engineered CRISPR-Cas Systems 
       and Methods for Sensitive and Specific Diagnostics

<400>  200
aaccaaaaac ttgtccatta gcaca                                             25


<210>  201
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificial sequences generated for Engineered CRISPR-Cas Systems 
       and Methods for Sensitive and Specific Diagnostics

<400>  201
acctagacca ccacttaacc ga                                                22


<210>  202
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificial sequences generated for Engineered CRISPR-Cas Systems 
       and Methods for Sensitive and Specific Diagnostics

<400>  202
acactatgcg agcagaaggg ta                                                22


<210>  203
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificial sequences generated for Engineered CRISPR-Cas Systems 
       and Methods for Sensitive and Specific Diagnostics

<400>  203
tgatttgagt gttgtcaatg ccaga                                             25


<210>  204
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificial sequences generated for Engineered CRISPR-Cas Systems 
       and Methods for Sensitive and Specific Diagnostics

<400>  204
cttttctcca agcagggtta cgt                                               23


<210>  205
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificial sequences generated for Engineered CRISPR-Cas Systems 
       and Methods for Sensitive and Specific Diagnostics

<400>  205
tgatagagac ctttatgaca agttgca                                           27


<210>  206
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificial sequences generated for Engineered CRISPR-Cas Systems 
       and Methods for Sensitive and Specific Diagnostics

<400>  206
ggtaccaaca gcttctctag tagc                                              24


<210>  207
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificial sequences generated for Engineered CRISPR-Cas Systems 
       and Methods for Sensitive and Specific Diagnostics

<400>  207
ggcacatggc tttgagttga ca                                                22


<210>  208
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificial sequences generated for Engineered CRISPR-Cas Systems 
       and Methods for Sensitive and Specific Diagnostics

<400>  208
gttgaacctt tctacaagcc gc                                                22


<210>  209
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificial sequences generated for Engineered CRISPR-Cas Systems 
       and Methods for Sensitive and Specific Diagnostics

<400>  209
tcgatagata tcctgctaat tccattgt                                          28


<210>  210
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificial sequences generated for Engineered CRISPR-Cas Systems 
       and Methods for Sensitive and Specific Diagnostics

<400>  210
agtcttgtaa aagtgttcca gaggt                                             25


<210>  211
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificial sequences generated for Engineered CRISPR-Cas Systems 
       and Methods for Sensitive and Specific Diagnostics

<400>  211
gggtgtggac attgctgcta at                                                22


<210>  212
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificial sequences generated for Engineered CRISPR-Cas Systems 
       and Methods for Sensitive and Specific Diagnostics

<400>  212
tcaatttcca tttgactcct gggt                                              24


<210>  213
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificial sequences generated for Engineered CRISPR-Cas Systems 
       and Methods for Sensitive and Specific Diagnostics

<400>  213
acaggttcat ctaagtgtgt gtgt                                              24


<210>  214
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificial sequences generated for Engineered CRISPR-Cas Systems 
       and Methods for Sensitive and Specific Diagnostics

<400>  214
ctcctttatc agaaccagca cca                                               23


<210>  215
<211>  29
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificial sequences generated for Engineered CRISPR-Cas Systems 
       and Methods for Sensitive and Specific Diagnostics

<400>  215
acaaaagaaa atgactctaa agagggttt                                         29


<210>  216
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificial sequences generated for Engineered CRISPR-Cas Systems 
       and Methods for Sensitive and Specific Diagnostics

<400>  216
tgaccttctt ttaaagacat aacagcag                                          28


<210>  217
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificial sequences generated for Engineered CRISPR-Cas Systems 
       and Methods for Sensitive and Specific Diagnostics

<400>  217
acacgtggtg tttattaccc tgac                                              24


<210>  218
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificial sequences generated for Engineered CRISPR-Cas Systems 
       and Methods for Sensitive and Specific Diagnostics

<400>  218
actctgaact cactttccat ccaac                                             25


<210>  219
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificial sequences generated for Engineered CRISPR-Cas Systems 
       and Methods for Sensitive and Specific Diagnostics

<400>  219
acatcactag gtttcaaact ttacttgc                                          28


<210>  220
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificial sequences generated for Engineered CRISPR-Cas Systems 
       and Methods for Sensitive and Specific Diagnostics

<400>  220
gcaacacagt tgctgattct cttc                                              24


<210>  221
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificial sequences generated for Engineered CRISPR-Cas Systems 
       and Methods for Sensitive and Specific Diagnostics

<400>  221
agggcaaact ggaaagattg ct                                                22


<210>  222
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificial sequences generated for Engineered CRISPR-Cas Systems 
       and Methods for Sensitive and Specific Diagnostics

<400>  222
gggcaaactg gaaagattgc tga                                               23


<210>  223
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificial sequences generated for Engineered CRISPR-Cas Systems 
       and Methods for Sensitive and Specific Diagnostics

<400>  223
acacctgtgc ctgttaaacc at                                                22


<210>  224
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificial sequences generated for Engineered CRISPR-Cas Systems 
       and Methods for Sensitive and Specific Diagnostics

<400>  224
acctgtgcct gttaaaccat tga                                               23


<210>  225
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificial sequences generated for Engineered CRISPR-Cas Systems 
       and Methods for Sensitive and Specific Diagnostics

<400>  225
caacttactc ctacttggcg tgt                                               23


<210>  226
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificial sequences generated for Engineered CRISPR-Cas Systems 
       and Methods for Sensitive and Specific Diagnostics

<400>  226
tgtgtacaaa aactgccata ttgca                                             25


<210>  227
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificial sequences generated for Engineered CRISPR-Cas Systems 
       and Methods for Sensitive and Specific Diagnostics

<400>  227
ttgccttggt gatattgctg ct                                                22


<210>  228
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificial sequences generated for Engineered CRISPR-Cas Systems 
       and Methods for Sensitive and Specific Diagnostics

<400>  228
tggagctaag ttgtttaaca agcg                                              24


<210>  229
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificial sequences generated for Engineered CRISPR-Cas Systems 
       and Methods for Sensitive and Specific Diagnostics

<400>  229
gggctatcat cttatgtcct tccct                                             25


<210>  230
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificial sequences generated for Engineered CRISPR-Cas Systems 
       and Methods for Sensitive and Specific Diagnostics

<400>  230
tgccagagat gtcacctaaa tcaa                                              24


<210>  231
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificial sequences generated for Engineered CRISPR-Cas Systems 
       and Methods for Sensitive and Specific Diagnostics

<400>  231
tgctgtagtt gtctcaaggg ct                                                22


<210>  232
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificial sequences generated for Engineered CRISPR-Cas Systems 
       and Methods for Sensitive and Specific Diagnostics

<400>  232
aggtgtgagt aaactgttac aaacaac                                           27


<210>  233
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificial sequences generated for Engineered CRISPR-Cas Systems 
       and Methods for Sensitive and Specific Diagnostics

<400>  233
tcaggtgatg gcacaacaag tc                                                22


<210>  234
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificial sequences generated for Engineered CRISPR-Cas Systems 
       and Methods for Sensitive and Specific Diagnostics

<400>  234
acgaaagcaa gaaaaagaag tacgc                                             25


<210>  235
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificial sequences generated for Engineered CRISPR-Cas Systems 
       and Methods for Sensitive and Specific Diagnostics

<400>  235
ccatggcaga ttccaacggt ac                                                22


<210>  236
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificial sequences generated for Engineered CRISPR-Cas Systems 
       and Methods for Sensitive and Specific Diagnostics

<400>  236
tggtcagaat agtgccatgg agt                                               23


<210>  237
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificial sequences generated for Engineered CRISPR-Cas Systems 
       and Methods for Sensitive and Specific Diagnostics

<400>  237
acacagacca ttccagtagc agt                                               23


<210>  238
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificial sequences generated for Engineered CRISPR-Cas Systems 
       and Methods for Sensitive and Specific Diagnostics

<400>  238
tgaaatggtg aattgccctc gt                                                22


<210>  239
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificial sequences generated for Engineered CRISPR-Cas Systems 
       and Methods for Sensitive and Specific Diagnostics

<400>  239
tttgtgcttt ttagcctttc tgct                                              24


<210>  240
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificial sequences generated for Engineered CRISPR-Cas Systems 
       and Methods for Sensitive and Specific Diagnostics

<400>  240
aggttcctgg caattaattg taaaagg                                           27


<210>  241
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificial sequences generated for Engineered CRISPR-Cas Systems 
       and Methods for Sensitive and Specific Diagnostics

<400>  241
ggccccaagg tttacccaat aa                                                22


<210>  242
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificial sequences generated for Engineered CRISPR-Cas Systems 
       and Methods for Sensitive and Specific Diagnostics

<400>  242
tttggcaatg ttgttccttg agg                                               23


<210>  243
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificial sequences generated for Engineered CRISPR-Cas Systems 
       and Methods for Sensitive and Specific Diagnostics

<400>  243
gccaacaaca acaaggccaa ac                                                22


<210>  244
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificial sequences generated for Engineered CRISPR-Cas Systems 
       and Methods for Sensitive and Specific Diagnostics

<400>  244
taggctctgt tggtgggaat gt                                                22


<210>  245
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificial sequences generated for Engineered CRISPR-Cas Systems 
       and Methods for Sensitive and Specific Diagnostics

<400>  245
aacaattgca acaatccatg agca                                              24


<210>  246
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificial sequences generated for Engineered CRISPR-Cas Systems 
       and Methods for Sensitive and Specific Diagnostics

<400>  246
ttctcctaag aagctattaa aatcacatgg                                        30


