                         SEQUENCE LISTING

<110>  Nanyang Technological University
 
<120>  HUMANIZED MONOCLONAL ANTIBODY AGAINST cANGPTL4

<130>  P119911

<150>  SG10202003838X
<151>  2020-04-27

<160>  24    

<170>  PatentIn version 3.5

<210>  1
<211>  7
<212>  PRT
<213>  Artificial

<220>
<223>  VH CDR1

<400>  1

Thr Ser Gly Met Gly Val Gly 
1               5           


<210>  2
<211>  16
<212>  PRT
<213>  Artificial

<220>
<223>  VH CDR2

<400>  2

His Ile Trp Trp Asp Asp Asp Lys Tyr Tyr Asn Pro Ser Leu Lys Ser 
1               5                   10                  15      


<210>  3
<211>  9
<212>  PRT
<213>  Artificial

<220>
<223>  VH CDR3

<400>  3

Lys Asp Tyr Gly Ser Ser Tyr Asp Tyr 
1               5                   


<210>  4
<211>  15
<212>  PRT
<213>  Artificial

<220>
<223>  VL CDR1

<400>  4

Lys Ala Ser Gln Ser Val Asp Tyr Asp Gly Asp Ser Tyr Leu Asn 
1               5                   10                  15  


<210>  5
<211>  7
<212>  PRT
<213>  Artificial

<220>
<223>  VL CDR2

<400>  5

Thr Ala Ser Asn Leu Glu Ser 
1               5           


<210>  6
<211>  8
<212>  PRT
<213>  Artificial

<220>
<223>  VL CDR3


<220>
<221>  MISC_FEATURE
<222>  (1)..(1)
<223>  Xaa can be Phe (F) or Gln (Q)

<400>  6

Xaa Gln Ser Asn Glu Asp Pro Trp 
1               5               


<210>  7
<211>  119
<212>  PRT
<213>  Artificial

<220>
<223>  VH domain ANGH1

<400>  7

Gln Ile Thr Leu Lys Glu Ser Gly Pro Thr Leu Val Lys Pro Thr Gln 
1               5                   10                  15      


Thr Leu Thr Leu Thr Cys Thr Phe Ser Gly Phe Ser Leu Ser Thr Ser 
            20                  25                  30          


Gly Met Gly Val Gly Trp Ile Arg Gln Pro Pro Gly Lys Ala Leu Glu 
        35                  40                  45              


Trp Leu Ala His Ile Trp Trp Asp Asp Asp Lys Tyr Tyr Asn Pro Ser 
    50                  55                  60                  


Leu Lys Ser Arg Leu Thr Ile Thr Lys Asp Thr Ser Lys Asn Gln Val 
65                  70                  75                  80  


Val Leu Thr Met Thr Asn Met Asp Pro Val Asp Thr Ala Thr Tyr Tyr 
                85                  90                  95      


Cys Ala Arg Lys Asp Tyr Gly Ser Ser Tyr Asp Tyr Trp Gly Gln Gly 
            100                 105                 110         


Thr Leu Val Thr Val Ser Ser 
        115                 


<210>  8
<211>  111
<212>  PRT
<213>  Artificial

<220>
<223>  VL domain ANGK1

<400>  8

Asp Ile Val Met Thr Gln Ser Pro Asp Ser Leu Ala Val Ser Leu Gly 
1               5                   10                  15      


Glu Arg Ala Thr Ile Asn Cys Lys Ala Ser Gln Ser Val Asp Tyr Asp 
            20                  25                  30          


Gly Asp Ser Tyr Leu Asn Trp Tyr Gln Gln Lys Pro Gly Gln Pro Pro 
        35                  40                  45              


Lys Leu Leu Ile Tyr Thr Ala Ser Asn Leu Glu Ser Gly Val Pro Asp 
    50                  55                  60                  


Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser 
65                  70                  75                  80  


Ser Leu Gln Ala Glu Asp Val Ala Val Tyr Tyr Cys Gln Gln Ser Asn 
                85                  90                  95      


Glu Asp Pro Trp Thr Phe Gly Gln Gly Thr Lys Val Glu Ile Lys 
            100                 105                 110     


<210>  9
<211>  119
<212>  PRT
<213>  Artificial

<220>
<223>  VH domain ANGH2

<400>  9

Gln Val Thr Leu Lys Glu Ser Gly Pro Val Leu Val Lys Pro Thr Glu 
1               5                   10                  15      


Thr Leu Thr Leu Thr Cys Thr Val Ser Gly Phe Ser Leu Ser Thr Ser 
            20                  25                  30          


Gly Met Gly Val Gly Trp Ile Arg Gln Pro Pro Gly Lys Ala Leu Glu 
        35                  40                  45              


Trp Leu Ala His Ile Trp Trp Asp Asp Asp Lys Tyr Tyr Asn Pro Ser 
    50                  55                  60                  


Leu Lys Ser Arg Leu Thr Ile Ser Lys Asp Thr Ser Lys Ser Gln Val 
65                  70                  75                  80  


Val Leu Thr Met Thr Asn Met Asp Pro Val Asp Thr Ala Thr Tyr Tyr 
                85                  90                  95      


Cys Ala Arg Lys Asp Tyr Gly Ser Ser Tyr Asp Tyr Trp Gly Gln Gly 
            100                 105                 110         


Thr Leu Val Thr Val Ser Ser 
        115                 


<210>  10
<211>  111
<212>  PRT
<213>  Artificial

<220>
<223>  VL domain ANGK2

<400>  10

Glu Ile Val Leu Thr Gln Ser Pro Ala Thr Leu Ser Leu Ser Pro Gly 
1               5                   10                  15      


Glu Arg Ala Thr Leu Ser Cys Lys Ala Ser Gln Ser Val Asp Tyr Asp 
            20                  25                  30          


Gly Asp Ser Tyr Leu Asn Trp Tyr Gln Gln Lys Pro Gly Gln Ala Pro 
        35                  40                  45              


Arg Leu Leu Ile Tyr Thr Ala Ser Asn Leu Glu Ser Gly Ile Pro Ala 
    50                  55                  60                  


Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser 
65                  70                  75                  80  


Ser Leu Glu Pro Glu Asp Phe Ala Val Tyr Tyr Cys Gln Gln Ser Asn 
                85                  90                  95      


Glu Asp Pro Trp Thr Phe Gly Gln Gly Thr Lys Leu Glu Ile Lys 
            100                 105                 110     


<210>  11
<211>  119
<212>  PRT
<213>  Artificial

<220>
<223>  VH domain ANGH3

<400>  11

Gln Val Thr Leu Lys Glu Ser Gly Pro Thr Leu Val Lys Pro Thr Gln 
1               5                   10                  15      


Thr Leu Thr Leu Thr Cys Thr Phe Ser Gly Phe Ser Leu Ser Thr Ser 
            20                  25                  30          


Gly Met Gly Val Gly Trp Ile Arg Gln Pro Pro Gly Lys Ala Leu Glu 
        35                  40                  45              


Trp Leu Ala His Ile Trp Trp Asp Asp Asp Lys Tyr Tyr Asn Pro Ser 
    50                  55                  60                  


Leu Lys Ser Arg Leu Thr Ile Ser Lys Asp Thr Ser Lys Asn Gln Val 
65                  70                  75                  80  


Val Leu Thr Met Thr Asn Met Asp Pro Val Asp Thr Ala Thr Tyr Tyr 
                85                  90                  95      


Cys Ala Arg Lys Asp Tyr Gly Ser Ser Tyr Asp Tyr Trp Gly Gln Gly 
            100                 105                 110         


Thr Thr Val Thr Val Ser Ser 
        115                 


<210>  12
<211>  111
<212>  PRT
<213>  Artificial

<220>
<223>  VL domain ANGK3

<400>  12

Asp Ile Val Met Thr Gln Ser Pro Leu Ser Leu Pro Val Thr Pro Gly 
1               5                   10                  15      


Glu Pro Ala Ser Ile Ser Cys Lys Ala Ser Gln Ser Val Asp Tyr Asp 
            20                  25                  30          


Gly Asp Ser Tyr Leu Asn Trp Tyr Leu Gln Lys Pro Gly Gln Ser Pro 
        35                  40                  45              


Arg Leu Leu Ile Tyr Thr Ala Ser Asn Leu Glu Ser Gly Val Pro Asp 
    50                  55                  60                  


Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Lys Ile Ser 
65                  70                  75                  80  


Arg Val Glu Ala Glu Asp Val Gly Val Tyr Tyr Cys Phe Gln Ser Asn 
                85                  90                  95      


Glu Asp Pro Trp Thr Phe Gly Gln Gly Thr Lys Leu Glu Ile Lys 
            100                 105                 110     


<210>  13
<211>  119
<212>  PRT
<213>  Artificial

<220>
<223>  VH domain ANGH4

<400>  13

Gln Ile Thr Leu Lys Glu Ser Gly Pro Thr Leu Val Lys Pro Thr Gln 
1               5                   10                  15      


Thr Leu Thr Leu Thr Cys Thr Phe Ser Gly Phe Ser Leu Ser Thr Ser 
            20                  25                  30          


Gly Met Gly Val Gly Trp Ile Arg Gln Pro Pro Gly Lys Ala Leu Glu 
        35                  40                  45              


Trp Leu Ala His Ile Trp Trp Asp Asp Asp Lys Tyr Tyr Asn Pro Ser 
    50                  55                  60                  


Leu Lys Ser Arg Leu Thr Ile Thr Lys Asp Thr Ser Lys Asn Gln Val 
65                  70                  75                  80  


Asp Leu Thr Met Thr Phe Met Asp Pro Trp Asp Thr Ala Thr Tyr Tyr 
                85                  90                  95      


Cys Ala His Lys Asp Tyr Gly Ser Ser Tyr Asp Tyr Trp Gly Gln Gly 
            100                 105                 110         


Thr Leu Val Thr Val Ser Ser 
        115                 


<210>  14
<211>  111
<212>  PRT
<213>  Artificial

<220>
<223>  VL domain ANGK4

<400>  14

Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly 
1               5                   10                  15      


Asp Arg Val Thr Ile Thr Cys Lys Ala Ser Gln Ser Val Asp Tyr Asp 
            20                  25                  30          


Gly Asp Ser Tyr Leu Asn Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro 
        35                  40                  45              


Asn Leu Leu Ile Tyr Thr Ala Ser Asn Leu Glu Ser Gly Val Pro Ser 
    50                  55                  60                  


Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser 
65                  70                  75                  80  


Ser Leu Gln Pro Glu Asp Phe Ala Thr Tyr Tyr Cys Gln Gln Ser Asn 
                85                  90                  95      


Glu Asp Pro Trp Thr Phe Gly Glu Gly Thr Lys Val Glu Ile Lys 
            100                 105                 110     


<210>  15
<211>  357
<212>  DNA
<213>  Artificial

<220>
<223>  VH domain ANGH1

<400>  15
caaattactc tgaaggagag cgggcccacc ctggtgaagc ccacccagac cctgaccctg       60

acctgtacct tcagcgggtt cagcctgagc accagcggga tgggggtggg gtggatcaga      120

cagccccccg ggaaggccct ggagtggctg gcccacatct ggtgggatga tgataagtac      180

tacaacccca gcctgaagag cagactgacc atcaccaagg ataccagcaa gaaccaggtg      240

gtgctgacca tgaccaacat ggatcccgtg gataccgcca cctactactg tgccagaaag      300

gattacggga gcagctacga ttactggggg caggggactc ttgttaccgt ttctagc         357


<210>  16
<211>  333
<212>  DNA
<213>  Artificial

<220>
<223>  VL domain ANGK1

<400>  16
gatatcgtga tgacccagag ccccgatagc ctggccgtga gcctggggga gagagccacc       60

atcaactgta aggccagcca gagcgtggat tacgatgggg atagctacct gaactggtac      120

cagcagaagc ccgggcagcc ccccaagctg ctgatctaca ccgccagcaa cctggagagc      180

ggggtgcccg atagattcag cgggagcggg agcgggaccg atttcaccct gaccatcagc      240

agcctgcagg ccgaggatgt ggccgtgtac tactgtcagc agagcaacga ggatccctgg      300

accttcgggc aggggactaa ggttgagatt aaa                                   333


<210>  17
<211>  357
<212>  DNA
<213>  Artificial

<220>
<223>  VH domain ANGH2

<400>  17
caagtgaccc tgaaggagag cgggcccgtg ctggtgaagc ccaccgagac cctgaccctg       60

acctgtaccg tgagcgggtt cagcctgagc accagcggga tgggggtggg gtggatcaga      120

cagccccccg ggaaggccct ggagtggctg gcccacatct ggtgggatga tgataagtac      180

tacaacccca gcctgaagag cagactgacc atcagcaagg ataccagcaa gagccaggtg      240

gtgctgacca tgaccaacat ggatcccgtg gataccgcca cctactactg tgccagaaag      300

gattacggga gcagctacga ttactggggg caggggactc ttgttaccgt ttctagc         357


<210>  18
<211>  333
<212>  DNA
<213>  Artificial

<220>
<223>  VL domain ANGK2

<400>  18
gagatcgtgc tgacccagag ccccgccacc ctgagcctga gccccgggga gagagccacc       60

ctgagctgta aggccagcca gagcgtggat tacgatgggg atagctacct gaactggtac      120

cagcagaagc ccgggcaggc ccccagactg ctgatctaca ccgccagcaa cctggagagc      180

gggatccccg ccagattcag cgggagcggg agcgggaccg atttcaccct gaccatcagc      240

agcctggagc ccgaggattt cgccgtgtac tactgtcagc agagcaacga ggatccctgg      300

accttcgggc aggggactaa gcttgagctt aaa                                   333


<210>  19
<211>  357
<212>  DNA
<213>  Artificial

<220>
<223>  VH domain ANGH3

<400>  19
caggtgaccc tgaaggagag cgggcccacc ctggtgaagc ccacccagac cctgaccctg       60

acctgtacct tcagcgggtt cagcctgagc accagcggga tgggggtggg gtggatcaga      120

cagccccccg ggaaggccct ggagtggctg gcccacatct ggtgggatga tgataagtac      180

tacaacccca gcctgaagag cagactgacc atcagcaagg ataccagcaa gaaccaggtg      240

gtgctgacca tgaccaacat ggatcccgtg gataccgcca cctactactg tgccagaaag      300

gattacggga gcagctacga ttactggggg caggggacga cggtcaccgt ctcctca         357


<210>  20
<211>  333
<212>  DNA
<213>  Artificial

<220>
<223>  VL domain ANGK3

<400>  20
gatatcgtga tgacccagag ccccctgagc ctgcccgtga cccccgggga gcccgccagc       60

atcagctgta aggccagcca gagcgtggat tacgatgggg atagctacct gaactggtac      120

ctgcagaagc ccgggcagag ccccagactg ctgatctaca ccgccagcaa cctggagagc      180

ggggtgcccg atagattcag cgggagcggg agcgggaccg atttcaccct gaagatcagc      240

agagtggagg ccgaggatgt gggggtgtac tactgtttcc agagcaacga ggatccctgg      300

accttcgggc aggggactaa gcttgagctt aaa                                   333


<210>  21
<211>  357
<212>  DNA
<213>  Artificial

<220>
<223>  VH domain ANGH4

<400>  21
cagatcaccc tgaaggagag cgggcccacc ctggtgaagc ccacccagac cctgaccctg       60

acctgtacct tcagcgggtt cagcctgagc accagcggga tgggggtggg gtggatcaga      120

cagccccccg ggaaggccct ggagtggctg gcccacatct ggtgggatga tgataagtac      180

tacaacccca gcctgaagag cagactgacc atcaccaagg ataccagcaa gaaccaggtg      240

gatctgacca tgaccttcat ggatccctgg gataccgcca cctactactg tgcccacaag      300

gattacggga gcagctacga ttactggggg caggggactc ttgttaccgt ttctagc         357


<210>  22
<211>  333
<212>  DNA
<213>  Artificial

<220>
<223>  VL domain ANGK4

<400>  22
gatatccaga tgacccagag ccccagcagc ctgagcgcca gcgtggggga tagagtgacc       60

atcacctgta aggccagcca gagcgtggat tacgatgggg atagctacct gaactggtac      120

cagcagaagc ccgggaaggc ccccaacctg ctgatctaca ccgccagcaa cctggagagc      180

ggggtgccca gcagattcag cgggagcggg agcgggaccg atttcaccct gaccatcagc      240

agcctgcagc ccgaggattt cgccacctac tactgtcagc agagcaacga ggatccctgg      300

accttcgggg aggggactaa ggttgagatt aaa                                   333


<210>  23
<211>  119
<212>  PRT
<213>  Artificial

<220>
<223>  murine mAb11F6C4 heavy chain variable domain

<400>  23

Gln Ile Thr Leu Lys Glu Ser Gly Pro Gly Ile Leu Lys Pro Ser Gln 
1               5                   10                  15      


Thr Leu Ser Leu Thr Cys Ser Phe Ser Gly Phe Ser Leu Ser Thr Ser 
            20                  25                  30          


Gly Met Gly Val Gly Trp Ile Arg Gln Pro Ser Gly Lys Gly Leu Glu 
        35                  40                  45              


Trp Leu Ala His Ile Trp Trp Asp Asp Asp Lys Tyr Tyr Asn Pro Ser 
    50                  55                  60                  


Leu Lys Ser Gln Leu Thr Ile Ser Lys Asp Thr Ser Arg Asn Gln Val 
65                  70                  75                  80  


Phe Leu Lys Ile Thr Ser Val Asp Thr Ala Asp Thr Ala Thr Tyr Tyr 
                85                  90                  95      


Cys Ala Arg Lys Asp Tyr Gly Ser Ser Tyr Asp Tyr Trp Gly Gln Gly 
            100                 105                 110         


Thr Thr Leu Thr Val Ser Ser 
        115                 


<210>  24
<211>  111
<212>  PRT
<213>  Artificial

<220>
<223>  murine mAB11F6C4 light chain variable domain

<400>  24

Asp Ile Val Leu Thr Gln Ser Pro Ala Ser Leu Ala Val Ser Leu Gly 
1               5                   10                  15      


Gln Arg Ala Thr Ile Ser Cys Lys Ala Ser Gln Ser Val Asp Tyr Asp 
            20                  25                  30          


Gly Asp Ser Tyr Leu Asn Trp Phe Gln Gln Lys Pro Gly Gln Pro Pro 
        35                  40                  45              


Lys Leu Leu Ile Tyr Thr Ala Ser Asn Leu Glu Ser Gly Ile Pro Ala 
    50                  55                  60                  


Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Asn Ile His 
65                  70                  75                  80  


Pro Val Glu Glu Glu Asp Ala Ala Thr Tyr Tyr Cys Gln Gln Ser Asn 
                85                  90                  95      


Glu Asp Pro Trp Thr Phe Gly Gly Gly Thr Thr Leu Glu Ile Lys 
            100                 105                 110     


