﻿               SEQUENCE LISTING

<110> RiboPro B.V.

<120> CU-depletie

<130> L/P169810PC00/JED

<140> 
<141> 

<160> 38

<170> BiSSAP 1.3.6

<210> 1
<211> 17
<212> DNA
<213> Artificial Sequence


<220> 
<223> T7 promoter

<400> 1
taatacgact cactata                                                   17


<210> 2
<211> 23
<212> DNA
<213> Artificial Sequence


<220> 
<223> Linear template Fwd primer

<400> 2
tacgtagcgc taatacgact cac                                            23


<210> 3
<211> 24
<212> DNA
<213> Artificial Sequence


<220> 
<223> Linear template Rvs primer

<400> 3
gtatcttatc atgtctgctc gaag                                           24


<210> 4
<211> 25
<212> DNA
<213> Artificial Sequence


<220> 
<223> UC-depleted and UG-depleted Fwd primer

<400> 4
ggaggtaata cgactcacta taggg                                          25


<210> 5
<211> 23
<212> DNA
<213> Artificial Sequence


<220> 
<223> UC-depleted and UG-depleted Rvs primer

<400> 5
ttttgtgttg gttgtgttgt ggt                                            23


<210> 6
<211> 23
<212> DNA
<213> Artificial Sequence


<220> 
<223> UC-depleted and UG-depleted Rvs primer

<400> 6
ttttctcttc cttctcttct cct                                            23


<210> 7
<211> 16
<212> DNA
<213> Artificial Sequence


<220> 
<223> 5’UTR DNA template

<400> 7
gggaaacgcc gccacc                                                    16


<210> 8
<211> 22
<212> DNA
<213> Artificial Sequence


<220> 
<223> 3’UTR DNA template

<400> 8
ggagaagaga aggaagagaa aa                                             22


<210> 9
<211> 22
<212> DNA
<213> Artificial Sequence


<220> 
<223> 3'UTR DNA template

<400> 9
ccacaacaca accaacacaa aa                                             22


<210> 10
<211> 14
<212> DNA
<213> Artificial Sequence


<220> 
<223> 5'UTR DNA template

<400> 10
gggaaactgc caag                                                      14


<210> 11
<211> 16
<212> DNA
<213> Artificial Sequence


<220> 
<223> 5'UTR DNA template

<400> 11
gggatacgcc gccacc                                                    16


<210> 12
<211> 23
<212> DNA
<213> Artificial Sequence


<220> 
<223> Rvs primer DNA template

<400> 12
ttttctcttc cttctcttct cct                                            23


<210> 13
<211> 23
<212> DNA
<213> Artificial Sequence


<220> 
<223> Rvs primer DNA template

<400> 13
ttttgtgttg gttgtgttgt ggt                                            23


<210> 14
<211> 600
<212> DNA
<213> Artificial Sequence


<220> 
<223> WT coding sequence secreted NanoLuc

<400> 14
atgaactcct tctccacaag cgccttcggt ccagttgcct tctccctggg cctgctcctg      60

gtgttgcctg ctgccttccc tgccccagtc ttcacactcg aagatttcgt tggggactgg     120

cgacagacag ccggctacaa cctggaccaa gtccttgaac agggaggtgt gtccagtttg     180

tttcagaatc tcggggtgtc cgtaactccg atccaaagga ttgtcctgag cggtgaaaat     240

gggctgaaga tcgacatcca tgtcatcatc ccgtatgaag gtctgagcgg cgaccaaatg     300

ggccagatcg aaaaaatttt taaggtggtg taccctgtgg atgatcatca ctttaaggtg     360

atcctgcact atggcacact ggtaatcgac ggggttacgc cgaacatgat cgactatttc     420

ggacggccgt atgaaggcat cgccgtgttc gacggcaaaa agatcactgt aacagggacc     480

ctgtggaacg gcaacaaaat tatcgacgag cgcctgatca accccgacgg ctccctgctg     540

ttccgagtaa ccatcaacgg agtgaccggc tggcggctgt gcgaacgcat tctggcgtaa     600


<210> 15
<211> 579
<212> DNA
<213> Mus musculus


<220> 
<223> WT coding sequence murine Erythropoietin

<400> 15
atgggggtgc ccgaacgtcc caccctgctg cttttactct ccttgctact gattcctctg      60

ggcctcccag tcctctgtgc tcccccacgc ctcatctgcg acagtcgagt tctggagagg     120

tacatcttag aggccaagga ggcagaaaat gtcacgatgg gttgtgcaga aggtcccaga     180

ctgagtgaaa atattacagt cccagatacc aaagtcaact tctatgcttg gaaaagaatg     240

gaggtggaag aacaggccat agaagtttgg caaggcctgt ccctgctctc agaagccatc     300

ctgcaggccc aggccctgct agccaattcc tcccagccac cagagaccct tcagcttcat     360

atagacaaag ccatcagtgg tctacgtagc ctcacttcac tgcttcgggt actgggagct     420

cagaaggaat tgatgtcgcc tccagatacc accccacctg ctccactccg aacactcaca     480

gtggatactt tctgcaagct cttccgggtc tacgccaact tcctccgggg gaaactgaag     540

ctgtacacgg gagaggtctg caggagaggg gacaggtga                            579


<210> 16
<211> 720
<212> DNA
<213> Aequorea victoria


<220> 
<223> WT coding sequence eGFP

<400> 16
atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac      60

ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac     120

ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc     180

ctcgtgacca ccctgaccta cggcgtgcag tgcttcagcc gctaccccga ccacatgaag     240

cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatctcc     300

ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg     360

gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac     420

aagctggagt acaactacaa cagccacaac gtctatatca tggccgacaa gcagaagaac     480

ggcatcaagg ccaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc     540

gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac     600

tacctgagca cccagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc     660

ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaagtaa     720


<210> 17
<211> 192
<212> PRT
<213> Mus musculus


<220> 
<223> murine EPO protein

<400> 17
Met Gly Val Pro Glu Arg Pro Thr Leu Leu Leu Leu Leu Ser Leu Leu 
1               5                   10                  15      
Leu Ile Pro Leu Gly Leu Pro Val Leu Cys Ala Pro Pro Arg Leu Ile 
            20                  25                  30          
Cys Asp Ser Arg Val Leu Glu Arg Tyr Ile Leu Glu Ala Lys Glu Ala 
        35                  40                  45              
Glu Asn Val Thr Met Gly Cys Ala Glu Gly Pro Arg Leu Ser Glu Asn 
    50                  55                  60                  
Ile Thr Val Pro Asp Thr Lys Val Asn Phe Tyr Ala Trp Lys Arg Met 
65                  70                  75                  80  
Glu Val Glu Glu Gln Ala Ile Glu Val Trp Gln Gly Leu Ser Leu Leu 
                85                  90                  95      
Ser Glu Ala Ile Leu Gln Ala Gln Ala Leu Leu Ala Asn Ser Ser Gln 
            100                 105                 110         
Pro Pro Glu Thr Leu Gln Leu His Ile Asp Lys Ala Ile Ser Gly Leu 
        115                 120                 125             
Arg Ser Leu Thr Ser Leu Leu Arg Val Leu Gly Ala Gln Lys Glu Leu 
    130                 135                 140                 
Met Ser Pro Pro Asp Thr Thr Pro Pro Ala Pro Leu Arg Thr Leu Thr 
145                 150                 155                 160 
Val Asp Thr Phe Cys Lys Leu Phe Arg Val Tyr Ala Asn Phe Leu Arg 
                165                 170                 175     
Gly Lys Leu Lys Leu Tyr Thr Gly Glu Val Cys Arg Arg Gly Asp Arg 
            180                 185                 190         



<210> 18
<211> 239
<212> PRT
<213> Aequorea victoria


<220> 
<223> eGFP protein

<400> 18
Met Val Ser Lys Gly Glu Glu Leu Phe Thr Gly Val Val Pro Ile Leu 
1               5                   10                  15      
Val Glu Leu Asp Gly Asp Val Asn Gly His Lys Phe Ser Val Ser Gly 
            20                  25                  30          
Glu Gly Glu Gly Asp Ala Thr Tyr Gly Lys Leu Thr Leu Lys Phe Ile 
        35                  40                  45              
Cys Thr Thr Gly Lys Leu Pro Val Pro Trp Pro Thr Leu Val Thr Thr 
    50                  55                  60                  
Leu Thr Tyr Gly Val Gln Cys Phe Ser Arg Tyr Pro Asp His Met Lys 
65                  70                  75                  80  
Gln His Asp Phe Phe Lys Ser Ala Met Pro Glu Gly Tyr Val Gln Glu 
                85                  90                  95      
Arg Thr Ile Ser Phe Lys Asp Asp Gly Asn Tyr Lys Thr Arg Ala Glu 
            100                 105                 110         
Val Lys Phe Glu Gly Asp Thr Leu Val Asn Arg Ile Glu Leu Lys Gly 
        115                 120                 125             
Ile Asp Phe Lys Glu Asp Gly Asn Ile Leu Gly His Lys Leu Glu Tyr 
    130                 135                 140                 
Asn Tyr Asn Ser His Asn Val Tyr Ile Met Ala Asp Lys Gln Lys Asn 
145                 150                 155                 160 
Gly Ile Lys Ala Asn Phe Lys Ile Arg His Asn Ile Glu Asp Gly Ser 
                165                 170                 175     
Val Gln Leu Ala Asp His Tyr Gln Gln Asn Thr Pro Ile Gly Asp Gly 
            180                 185                 190         
Pro Val Leu Leu Pro Asp Asn His Tyr Leu Ser Thr Gln Ser Ala Leu 
        195                 200                 205             
Ser Lys Asp Pro Asn Glu Lys Arg Asp His Met Val Leu Leu Glu Phe 
    210                 215                 220                 
Val Thr Ala Ala Gly Ile Thr Leu Gly Met Asp Glu Leu Tyr Lys 
225                 230                 235                 

<210> 19
<211> 199
<212> PRT
<213> Artificial Sequence


<220> 
<223> Nanoluciferase protein

<400> 19
Met Asn Ser Phe Ser Thr Ser Ala Phe Gly Pro Val Ala Phe Ser Leu 
1               5                   10                  15      
Gly Leu Leu Leu Val Leu Pro Ala Ala Phe Pro Ala Pro Val Phe Thr 
            20                  25                  30          
Leu Glu Asp Phe Val Gly Asp Trp Arg Gln Thr Ala Gly Tyr Asn Leu 
        35                  40                  45              
Asp Gln Val Leu Glu Gln Gly Gly Val Ser Ser Leu Phe Gln Asn Leu 
    50                  55                  60                  
Gly Val Ser Val Thr Pro Ile Gln Arg Ile Val Leu Ser Gly Glu Asn 
65                  70                  75                  80  
Gly Leu Lys Ile Asp Ile His Val Ile Ile Pro Tyr Glu Gly Leu Ser 
                85                  90                  95      
Gly Asp Gln Met Gly Gln Ile Glu Lys Ile Phe Lys Val Val Tyr Pro 
            100                 105                 110         
Val Asp Asp His His Phe Lys Val Ile Leu His Tyr Gly Thr Leu Val 
        115                 120                 125             
Ile Asp Gly Val Thr Pro Asn Met Ile Asp Tyr Phe Gly Arg Pro Tyr 
    130                 135                 140                 
Glu Gly Ile Ala Val Phe Asp Gly Lys Lys Ile Thr Val Thr Gly Thr 
145                 150                 155                 160 
Leu Trp Asn Gly Asn Lys Ile Ile Asp Glu Arg Leu Ile Asn Pro Asp 
                165                 170                 175     
Gly Ser Leu Leu Phe Arg Val Thr Ile Asn Gly Val Thr Gly Trp Arg 
            180                 185                 190         
Leu Cys Glu Arg Ile Leu Ala 
        195                 

<210> 20
<211> 672
<212> DNA
<213> Artificial Sequence


<220> 
<223> Secreted NanoLuc – WT

<400> 20
gggatacgcc gccaccatga actccttctc cacaagcgcc ttcggtccag ttgccttctc      60

cctgggcctg ctcctggtgt tgcctgctgc cttccctgcc ccagtcttca cactcgaaga     120

tttcgttggg gactggcgac agacagccgg ctacaacctg gaccaagtcc ttgaacaggg     180

aggtgtgtcc agtttgtttc agaatctcgg ggtgtccgta actccgatcc aaaggattgt     240

cctgagcggt gaaaatgggc tgaagatcga catccatgtc atcatcccgt atgaaggtct     300

gagcggcgac caaatgggcc agatcgaaaa aatttttaag gtggtgtacc ctgtggatga     360

tcatcacttt aaggtgatcc tgcactatgg cacactggta atcgacgggg ttacgccgaa     420

catgatcgac tatttcggac ggccgtatga aggcatcgcc gtgttcgacg gcaaaaagat     480

cactgtaaca gggaccctgt ggaacggcaa caaaattatc gacgagcgcc tgatcaaccc     540

cgacggctcc ctgctgttcc gagtaaccat caacggagtg accggctggc ggctgtgcga     600

acgcattctg gcgtaaggcc gcgactctag agtcggggcg gccggccgct tcgagcagac     660

atgataagat ac                                                         672


<210> 21
<211> 638
<212> DNA
<213> Artificial Sequence


<220> 
<223> Secreted NanoLuc – WT (control to other mRNAs)

<400> 21
gggaaacgcc gccaccatga actccttctc cacaagcgcc ttcggtccag ttgccttctc      60

cctgggcctg ctcctggtgt tgcctgctgc cttccctgcc ccagtcttca cactcgaaga     120

tttcgttggg gactggcgac agacagccgg ctacaacctg gaccaagtcc ttgaacaggg     180

aggtgtgtcc agtttgtttc agaatctcgg ggtgtccgta actccgatcc aaaggattgt     240

cctgagcggt gaaaatgggc tgaagatcga catccatgtc atcatcccgt atgaaggtct     300

gagcggcgac caaatgggcc agatcgaaaa aatttttaag gtggtgtacc ctgtggatga     360

tcatcacttt aaggtgatcc tgcactatgg cacactggta atcgacgggg ttacgccgaa     420

catgatcgac tatttcggac ggccgtatga aggcatcgcc gtgttcgacg gcaaaaagat     480

cactgtaaca gggaccctgt ggaacggcaa caaaattatc gacgagcgcc tgatcaaccc     540

cgacggctcc ctgctgttcc gagtaaccat caacggagtg accggctggc ggctgtgcga     600

acgcattctg gcgtaaggag aagagaagga agagaaaa                             638


<210> 22
<211> 638
<212> DNA
<213> Artificial Sequence


<220> 
<223> Secreted NanoLuc – U-depleted (maximum exchange)

<400> 22
gggaaacgcc gccaccatga actccttctc cacaagcgcc ttcggaccag tcgccttctc      60

cctgggcctg ctcctggtgc tccccgcagc cttccccgcc ccagtcttca cactcgaaga     120

cttcgtcggg gactggcgac agacagccgg ctacaacctg gaccaagtcc tcgaacaggg     180

aggagtgtcc agcctcttcc agaacctcgg ggtgtccgta actccgatcc aaaggatcgt     240

cctgagcgga gaaaacgggc tgaagatcga catccacgtc atcatcccgt acgaaggact     300

gagcggcgac caaatgggcc agatcgaaaa aatcttcaag gtggtgtacc ccgtggacga     360

ccaccacttc aaggtgatcc tgcactacgg cacactggta atcgacgggg tcacgccgaa     420

catgatcgac tacttcggac ggccgtacga aggcatcgcc gtgttcgacg gcaaaaagat     480

cactgtaaca gggaccctgt ggaacggcaa caaaatcatc gacgagcgcc tgatcaaccc     540

cgacggctcc ctgctgttcc gagtaaccat caacggagtg accggctggc ggctgtgcga     600

acgcatcctg gcgtaaccac aacacaacca acacaaaa                             638


<210> 23
<211> 638
<212> DNA
<213> Artificial Sequence


<220> 
<223> Secreted NanoLuc – UC-depleted (maximum exchange)

<400> 23
gggaaacgcc gccaccatga acagcttcag cacaagcgca ttcggaccag tggcattcag      60

cctgggactg ctgctggtgc tgccagcagc attcccagca ccagtcttca cactggagga     120

cttcgtgggg gactggagac agacagcagg atacaacctg gaccaggtcc tggagcaggg     180

aggagtgagc agcctgttcc agaacctggg ggtgagcgtg acaccaatcc agagaatcgt     240

cctgagcgga gagaacgggc tgaagatcga catccacgtc atcatcccat acgagggact     300

gagcggagac cagatgggac agatcgagaa gatcttcaag gtggtgtacc cagtggacga     360

ccaccacttc aaggtgatcc tgcactacgg aacactggtg atcgacgggg tgacaccaaa     420

catgatcgac tacttcggaa gaccatacga gggaatcgca gtgttcgacg gaaagaagat     480

cacagtgaca gggacactgt ggaacggaaa caagatcatc gacgagagac tgatcaaccc     540

agacggaagc ctgctgttca gagtgacaat caacggagtg acaggatgga gactgtgcga     600

gagaatcctg gcataaggag aagagaagga agagaaaa                             638


<210> 24
<211> 638
<212> DNA
<213> Artificial Sequence


<220> 
<223> Secreted NanoLuc – UG-depleted (maximum exchange)

<400> 24
gggaaacgcc gccaccatga actccttctc cacaagcgcc ttcggaccag tcgccttctc      60

cctcggcctc ctcctcgtcc tcccagcagc cttcccagcc ccagtcttca cactcgaaga     120

cttcgtcgga gactggcgac aaacagccgg ctacaacctc gaccaagtcc tcgaacaagg     180

aggagtctcc agcctcttcc aaaacctcgg agtctccgta acaccaatcc aaagaatcgt     240

cctcagcgga gaaaacggac tcaaaatcga catccacgtc atcatcccat acgaaggact     300

cagcggcgac caaatgggcc aaatcgaaaa aatcttcaaa gtcgtctacc cagtcgacga     360

ccaccacttc aaagtcatcc tccactacgg cacactcgta atcgacggag tcacaccaaa     420

catgatcgac tacttcggac gcccatacga aggcatcgcc gtcttcgacg gcaaaaaaat     480

cacagtaaca ggaaccctct ggaacggcaa caaaatcatc gacgagcgcc tcatcaaccc     540

cgacggctcc ctcctcttcc gagtaaccat caacggagtc accggctggc gcctctgcga     600

acgcatcctc gcataaccac aacacaacca acacaaaa                             638


<210> 25
<211> 638
<212> DNA
<213> Artificial Sequence


<220> 
<223> Secreted NanoLuc – C-depleted (maximum exchange of only
      C-containing but not U-containing codons)

<400> 25
gggaaacgcc gccaccatga actccttctc cacaagcgca ttcggtccag ttgcattctc      60

cctgggactg ctcctggtgt tgcctgctgc attccctgca ccagtcttca cactcgaaga     120

tttcgttggg gactggcgac agacagcagg atacaacctg gaccaagtcc ttgaacaggg     180

aggtgtgtcc agtttgtttc agaatctcgg ggtgtccgta actccgatcc aaaggattgt     240

cctgagcggt gaaaatgggc tgaagatcga catccatgtc atcatcccgt atgaaggtct     300

gagcggagac caaatgggac agatcgaaaa aatttttaag gtggtgtacc ctgtggatga     360

tcatcacttt aaggtgatcc tgcactatgg aacactggta atcgacgggg ttacgccgaa     420

catgatcgac tatttcggac ggccgtatga aggaatcgca gtgttcgacg gaaaaaagat     480

cactgtaaca gggacactgt ggaacggaaa caaaattatc gacgagcggc tgatcaaccc     540

agacggatcc ctgctgttcc gagtaacaat caacggagtg acaggatggc ggctgtgcga     600

acggattctg gcgtaaggag aagagaagga agagaaaa                             638


<210> 26
<211> 638
<212> DNA
<213> Artificial Sequence


<220> 
<223> Secreted NanoLuc – C2-depleted (maximum exchange of all
      C-containing codons)

<400> 26
gggaaacgcc gccaccatga acagtttcag tacaagcgca ttcggtccag ttgcattcag      60

tctgggactg ctgctggtgt tgcctgctgc attccctgca ccagtgttca cactggaaga     120

tttcgttggg gactggcgac agacagcagg atacaacctg gaccaagtgc ttgaacaggg     180

aggtgtgagt agtttgtttc agaatctggg ggtgagtgta actccgatac aaaggattgt     240

gctgagcggt gaaaatgggc tgaagataga catacatgtg ataataccgt atgaaggtct     300

gagcggagac caaatgggac agatagaaaa aatttttaag gtggtgtacc ctgtggatga     360

tcatcacttt aaggtgatac tgcactatgg aacactggta atagacgggg ttacgccgaa     420

catgatagac tatttcggac ggccgtatga aggaatagca gtgttcgacg gaaaaaagat     480

aactgtaaca gggacactgt ggaacggaaa caaaattata gacgagcggc tgataaaccc     540

agacggaagt ctgctgttcc gagtaacaat aaacggagtg acaggatggc ggctgtgcga     600

acggattctg gcgtaaggag aagagaagga agagaaaa                             638


<210> 27
<211> 758
<212> DNA
<213> Aequorea victoria


<220> 
<223> eGFP – WT

<400> 27
gggatacgcc gccaccatgg tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat      60

cctggtcgag ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga     120

gggcgatgcc acctacggca agctgaccct gaagttcatc tgcaccaccg gcaagctgcc     180

cgtgccctgg cccaccctcg tgaccaccct gacctacggc gtgcagtgct tcagccgcta     240

ccccgaccac atgaagcagc acgacttctt caagtccgcc atgcccgaag gctacgtcca     300

ggagcgcacc atctccttca aggacgacgg caactacaag acccgcgccg aggtgaagtt     360

cgagggcgac accctggtga accgcatcga gctgaagggc atcgacttca aggaggacgg     420

caacatcctg gggcacaagc tggagtacaa ctacaacagc cacaacgtct atatcatggc     480

cgacaagcag aagaacggca tcaaggccaa cttcaagatc cgccacaaca tcgaggacgg     540

cagcgtgcag ctcgccgacc actaccagca gaacaccccc atcggcgacg gccccgtgct     600

gctgcccgac aaccactacc tgagcaccca gtccgccctg agcaaagacc ccaacgagaa     660

gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc gggatcactc tcggcatgga     720

cgagctgtac aagtaaggag aagagaagga agagaaaa                             758


<210> 28
<211> 758
<212> DNA
<213> Aequorea victoria


<220> 
<223> eGFP – U-depleted (maximum exchange)

<400> 28
gggatacgcc gccaccatgg tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat      60

cctggtcgag ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga     120

gggcgacgcc acctacggca agctgaccct gaagttcatc tgcaccaccg gcaagctgcc     180

cgtgccctgg cccaccctcg tgaccaccct gacctacggc gtgcagtgct tcagccgcta     240

ccccgaccac atgaagcagc acgacttctt caagtccgcc atgcccgaag gctacgtcca     300

ggagcgcacc atctccttca aggacgacgg caactacaag acccgcgccg aggtgaagtt     360

cgagggcgac accctggtga accgcatcga gctgaagggc atcgacttca aggaggacgg     420

caacatcctg gggcacaagc tggagtacaa ctacaacagc cacaacgtct acatcatggc     480

cgacaagcag aagaacggca tcaaggccaa cttcaagatc cgccacaaca tcgaggacgg     540

cagcgtgcag ctcgccgacc actaccagca gaacaccccc atcggcgacg gccccgtgct     600

gctgcccgac aaccactacc tgagcaccca gtccgccctg agcaaagacc ccaacgagaa     660

gcgcgaccac atggtcctgc tggagttcgt gaccgccgcc gggatcacac tcggcatgga     720

cgagctgtac aagtaaccac aacacaacca acacaaaa                             758


<210> 29
<211> 758
<212> DNA
<213> Aequorea victoria


<220> 
<223> eGFP – UC-depleted (maximum exchange)

<400> 29
gggatacgcc gccaccatgg tgagcaaggg ggaggagctg ttcacagggg tggtgccaat      60

cctggtcgag ctggacgggg acgtaaacgg gcacaagttc agcgtgagcg gggaggggga     120

gggggacgca acatacggga agctgacact gaagttcatc tgcacaacag ggaagctgcc     180

agtgccatgg ccaacactcg tgacaacact gacatacggg gtgcagtgct tcagcagata     240

cccagaccac atgaagcagc acgacttctt caagagcgca atgccagaag ggtacgtcca     300

ggagagaaca atcagcttca aggacgacgg gaactacaag acaagagcag aggtgaagtt     360

cgagggggac acactggtga acagaatcga gctgaagggg atcgacttca aggaggacgg     420

gaacatcctg gggcacaagc tggagtacaa ctacaacagc cacaacgtct acatcatggc     480

agacaagcag aagaacggga tcaaggcaaa cttcaagatc agacacaaca tcgaggacgg     540

gagcgtgcag ctcgcagacc actaccagca gaacacacca atcggggacg ggccagtgct     600

gctgccagac aaccactacc tgagcacaca gagcgcactg agcaaagacc caaacgagaa     660

gagagaccac atggtcctgc tggagttcgt gacagcagca gggatcactc tcgggatgga     720

cgagctgtac aagtaaggag aagagaagga agagaaaa                             758


<210> 30
<211> 758
<212> DNA
<213> Aequorea victoria


<220> 
<223> eGFP – UG-depleted (maximum exchange)

<400> 30
gggatacgcc gccaccatgg tcagcaaagg cgaagaactc ttcaccggag tcgtccccat      60

cctcgtcgaa ctcgacggcg acgtaaacgg ccacaaattc agcgtctccg gcgaaggcga     120

aggcgacgcc acctacggca aactcaccct caaattcatc tgcaccaccg gcaaactccc     180

cgtcccctgg cccaccctcg tcaccaccct cacctacggc gtccaatgct tcagccgcta     240

ccccgaccac atgaaacaac acgacttctt caaatccgcc atgcccgaag gctacgtcca     300

agaacgcacc atctccttca aagacgacgg caactacaaa acccgcgccg aagtcaaatt     360

cgaaggcgac accctcgtca accgcatcga actcaaaggc atcgacttca aagaagacgg     420

caacatcctc ggacacaaac tcgaatacaa ctacaacagc cacaacgtct acatcatggc     480

cgacaaacaa aaaaacggca tcaaagccaa cttcaaaatc cgccacaaca tcgaagacgg     540

cagcgtccaa ctcgccgacc actaccaaca aaacaccccc atcggcgacg gccccgtcct     600

cctccccgac aaccactacc tcagcaccca atccgccctc agcaaagacc ccaacgaaaa     660

acgcgaccac atggtcctcc tcgaattcgt caccgccgcc ggaatcacac tcggcatgga     720

cgaactctac aaataaccac aacacaacca acacaaaa                             758


<210> 31
<211> 758
<212> DNA
<213> Aequorea victoria


<220> 
<223> eGFP– C-depleted (maximum exchange of only C-containing but not
      U-containing codons)

<400> 31
gggatacgcc gccaccatgg tgagcaaggg agaggagctg ttcacagggg tggtgccaat      60

cctggtcgag ctggacggag acgtaaacgg acacaagttc agcgtgtccg gagagggaga     120

gggagatgca acatacggaa agctgacact gaagttcatc tgcacaacag gaaagctgcc     180

agtgccatgg ccaacactcg tgacaacact gacatacgga gtgcagtgct tcagccggta     240

cccagaccac atgaagcagc acgacttctt caagtccgca atgccagaag gatacgtcca     300

ggagcggaca atctccttca aggacgacgg aaactacaag acacgggcag aggtgaagtt     360

cgagggagac acactggtga accggatcga gctgaaggga atcgacttca aggaggacgg     420

aaacatcctg gggcacaagc tggagtacaa ctacaacagc cacaacgtct atatcatggc     480

agacaagcag aagaacggaa tcaaggcaaa cttcaagatc cggcacaaca tcgaggacgg     540

aagcgtgcag ctcgcagacc actaccagca gaacacacca atcggagacg gaccagtgct     600

gctgccagac aaccactacc tgagcacaca gtccgcactg agcaaagacc caaacgagaa     660

gcgggatcac atggtcctgc tggagttcgt gacagcagca gggatcactc tcggaatgga     720

cgagctgtac aagtaaggag aagagaagga agagaaaa                             758


<210> 32
<211> 758
<212> DNA
<213> Aequorea victoria


<220> 
<223> eGFP – C2-depleted (maximum exchange of all C-containing codons)

<400> 32
gggatacgcc gccaccatgg tgagcaaggg agaggagctg ttcacagggg tggtgccaat      60

actggtggag ctggacggag acgtaaacgg acacaagttc agcgtgagtg gagagggaga     120

gggagatgca acatacggaa agctgacact gaagttcata tgcaccacag gaaagctgcc     180

agtgccatgg ccaacactgg tgacaacact gacatacgga gtgcagtgct tcagccggta     240

cccagaccac atgaagcagc acgacttctt caagagtgca atgccagaag gatacgtgca     300

ggagcggaca ataagtttca aggacgacgg aaactacaag acacgggcag aggtgaagtt     360

cgagggagac acactggtga accggataga gctgaaggga atagacttca aggaggacgg     420

aaacatactg gggcacaagc tggagtacaa ctacaacagc cacaacgtgt atataatggc     480

agacaagcag aagaacggaa taaaggcaaa cttcaagata cggcacaaca tagaggacgg     540

aagcgtgcag ctggcagacc actaccagca gaacacacca ataggagacg gaccagtgct     600

gctgccagac aaccactacc tgagcacaca gagtgcactg agcaaagacc caaacgagaa     660

gcgggatcac atggtgctgc tggagttcgt gacagcagca gggataactc tgggaatgga     720

cgagctgtac aagtaaggag aagagaagga agagaaaa                             758


<210> 33
<211> 615
<212> DNA
<213> Mus musculus


<220> 
<223> mEPO – WT

<400> 33
gggaaactgc caagatgggg gtgcccgaac gtcccaccct gctgctttta ctctccttgc      60

tactgattcc tctgggcctc ccagtcctct gtgctccccc acgcctcatc tgcgacagtc     120

gagttctgga gaggtacatc ttagaggcca aggaggcaga aaatgtcacg atgggttgtg     180

cagaaggtcc cagactgagt gaaaatatta cagtcccaga taccaaagtc aacttctatg     240

cttggaaaag aatggaggtg gaagaacagg ccatagaagt ttggcaaggc ctgtccctgc     300

tctcagaagc catcctgcag gcccaggccc tgctagccaa ttcctcccag ccaccagaga     360

cccttcagct tcatatagac aaagccatca gtggtctacg tagcctcact tcactgcttc     420

gggtactggg agctcagaag gaattgatgt cgcctccaga taccacccca cctgctccac     480

tccgaacact cacagtggat actttctgca agctcttccg ggtctacgcc aacttcctcc     540

gggggaaact gaagctgtac acgggagagg tctgcaggag aggggacagg tgaggagaag     600

agaaggaaga gaaaa                                                      615


<210> 34
<211> 615
<212> DNA
<213> Mus musculus


<220> 
<223> mEPO – U-depleted (maximum exchange)

<400> 34
gggaaactgc caagatgggg gtgcccgaac gacccaccct gctgctctta ctctccctcc      60

tactgatccc cctgggcctc ccagtcctct gcgcaccccc acgcctcatc tgcgacagcc     120

gagtcctgga gaggtacatc ttagaggcca aggaggcaga aaacgtcacg atgggatgcg     180

cagaaggacc cagactgagc gaaaacatca cagtcccaga caccaaagtc aacttctacg     240

catggaaaag aatggaggtg gaagaacagg ccatagaagt ctggcaaggc ctgtccctgc     300

tctcagaagc catcctgcag gcccaggccc tgctagccaa ctcctcccag ccaccagaga     360

ccctccagct ccacatagac aaagccatca gcggactacg aagcctcaca tcactgctcc     420

gggtactggg agcacagaag gaactcatgt cgcccccaga caccacccca cccgcaccac     480

tccgaacact cacagtggac acattctgca agctcttccg ggtctacgcc aacttcctcc     540

gggggaaact gaagctgtac acgggagagg tctgcaggag aggggacagg taaccacaac     600

acaaccaaca caaaa                                                      615


<210> 35
<211> 615
<212> DNA
<213> Mus musculus


<220> 
<223> mEPO – UC-depleted (maximum exchange)

<400> 35
gggaaactgc caagatgggg gtgccagaac gaccaacact gctgctccta ctcagcttgc      60

tactgatccc actggggctc ccagtcctct gcgcaccacc aagactcatc tgcgacagcc     120

gagtactgga gaggtacatc ctagaggcaa aggaggcaga aaacgtcacg atgggatgcg     180

cagaaggacc aagactgagc gaaaacatca cagtcccaga cacaaaagtc aacttctacg     240

catggaaaag aatggaggtg gaagaacagg caatagaagt atggcaaggg ctgagcctgc     300

tcagcgaagc aatcctgcag gcacaggcac tgctagcaaa cagcagccag ccaccagaga     360

cactccagct ccacatagac aaagcaatca gcggactacg aagcctcact agcctgctca     420

gggtactggg agcacagaag gaattgatgt cgccaccaga cacaacacca ccagcaccac     480

tccgaacact cacagtggac actttctgca agctcttcag ggtctacgca aacttcctca     540

gggggaaact gaagctgtac acgggagagg tctgcaggag aggggacagg tgaggagaag     600

agaaggaaga gaaaa                                                      615


<210> 36
<211> 615
<212> DNA
<213> Mus musculus


<220> 
<223> mEPO – UG-depleted (maximum exchange)

<400> 36
gggaaactgc caagatggga gtccccgaac gacccaccct cctcctctta ctctccctcc      60

tactcatccc actcggcctc ccagtcctct gcgcaccccc acgcctcatc tgcgacagcc     120

gagtcctcga aagatacatc ttagaagcca aagaagcaga aaacgtcaca atgggatgcg     180

cagaaggacc cagactcagc gaaaacatca cagtcccaga caccaaagtc aacttctacg     240

catggaaaag aatggaagtc gaagaacaag ccatagaagt ctggcaaggc ctctccctcc     300

tctcagaagc catcctccaa gcccaagccc tcctagccaa ctcctcccaa ccaccagaaa     360

ccctccaact ccacatagac aaagccatca gcggactacg aagcctcaca tcactcctcc     420

gcgtactcgg agcacaaaaa gaactcatgt caccaccaga caccacccca ccagcaccac     480

tccgaacact cacagtcgac acattctgca aactcttccg cgtctacgcc aacttcctcc     540

gcggaaaact caaactctac acaggagaag tctgcagaag aggagacaga taaccacaac     600

acaaccaaca caaaa                                                      615


<210> 37
<211> 615
<212> DNA
<213> Mus musculus


<220> 
<223> mEPO – C-depleted (maximum exchange of only C-containing but not
      U-containing codons)

<400> 37
gggaaactgc caagatgggg gtgccagaac gtccaacact gctgctttta ctctccttgc      60

tactgattcc tctgggactc ccagtcctct gtgctccacc acggctcatc tgcgacagtc     120

gagttctgga gaggtacatc ttagaggcaa aggaggcaga aaatgtcacg atgggttgtg     180

cagaaggtcc aagactgagt gaaaatatta cagtcccaga tacaaaagtc aacttctatg     240

cttggaaaag aatggaggtg gaagaacagg caatagaagt ttggcaagga ctgtccctgc     300

tctcagaagc aatcctgcag gcacaggcac tgctagcaaa ttcctcccag ccaccagaga     360

cacttcagct tcatatagac aaagcaatca gtggtctacg tagcctcact tcactgcttc     420

gggtactggg agctcagaag gaattgatgt cgcctccaga tacaacacca cctgctccac     480

tccgaacact cacagtggat actttctgca agctcttccg ggtctacgca aacttcctcc     540

gggggaaact gaagctgtac acgggagagg tctgcaggag aggggacagg tgaggagaag     600

agaaggaaga gaaaa                                                      615


<210> 38
<211> 615
<212> DNA
<213> Mus musculus


<220> 
<223> mEPO – C2-depleted (maximum exchange of all C-containing codons)

<400> 38
gggaaactgc caagatgggg gtgccagaac gtccaacact gctgctttta ctgagtttgc      60

tactgattcc tctgggactg ccagtgctgt gtgctccacc acggctgata tgcgacagtc     120

gagttctgga gaggtacata ttagaggcaa aggaggcaga aaatgtgacg atgggttgtg     180

cagaaggtcc aagactgagt gaaaatatta cagtgccaga tacaaaagtg aacttctatg     240

cttggaaaag aatggaggtg gaagaacagg caatagaagt ttggcaagga ctgagtctgc     300

tgagtgaagc aatactgcag gcacaggcac tgctagcaaa tagtagtcag ccaccagaga     360

cacttcagct tcatatagac aaagcaataa gtggtctacg tagcctgact agtctgcttc     420

gggtactggg agctcagaag gaattgatga gtcctccaga tacaacacca cctgctccac     480

tgcgaacact gacagtggat actttctgca agctgttccg ggtgtacgca aacttcctgc     540

gggggaaact gaagctgtac acgggagagg tgtgcaggag aggggacagg tgaggagaag     600

agaaggaaga gaaaa                                                      615


