                         SEQUENCE LISTING

<110>  Flagship Pioneering, Inc.
 
<120>  BIFUNCTIONAL MOLECULES AND METHODS OF USING THEREOF

<130>  127250-5008-WO

<160>  37    

<170>  PatentIn version 3.5

<210>  1
<211>  16
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  antisense oligonucleotide (ASO)

<400>  1
cguuaacuag gcuuua                                                       16


<210>  2
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  antisense oligonucleotide (ASO)

<400>  2
ggaagggaat cagcaggtat                                                   20


<210>  3
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  antisense oligonucleotide (ASO)

<400>  3
tcttgggccg aggctactga                                                   20


<210>  4
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  antisense oligonucleotide (ASO)

<400>  4
cctggggctg gtgcattttc                                                   20


<210>  5
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  antisense oligonucleotide (ASO) 1

<400>  5
cttggtaaga ctgttggtga                                                   20


<210>  6
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  antisense oligonucleotide (ASO) 2

<400>  6
tgtggaggtc tttgtgtctt                                                   20


<210>  7
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  antisense oligonucleotide (ASO) 3

<400>  7
aggtgtcgtc tatgctgtcc                                                   20


<210>  8
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  antisense oligonucleotide (ASO) 4

<400>  8
acggtggaat tgttgctggt                                                   20


<210>  9
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  antisense oligonucleotide (ASO) 5

<400>  9
tgtaggtcct tctgtttccc                                                   20


<210>  10
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  antisense oligonucleotide (ASO) 6

<400>  10
tgtaattaga ggagctcctt                                                   20


<210>  11
<211>  16
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  antisense oligonucleotide (ASO) 7

<400>  11
ggtacaagct ggaggt                                                       16


<210>  12
<211>  16
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  antisense oligonucleotide (ASO) 8

<400>  12
gtagttgtgc tgatgt                                                       16


<210>  13
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  antisense oligonucleotide (ASO) 9

<400>  13
aacctatggt tactccagac gag                                               23


<210>  14
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  antisense oligonucleotide (ASO) 10

<400>  14
aggtatttct aatacctaca ccc                                               23


<210>  15
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  antisense oligonucleotide (ASO) 11

<400>  15
ataggtggtc tctgatggtc                                                   20


<210>  16
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  antisense oligonucleotide (ASO) 12

<400>  16
gttgtcttgt tcttacagcc                                                   20


<210>  17
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  antisense oligonucleotide (ASO) 13

<400>  17
tataccagtg gttgtttagg                                                   20


<210>  18
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  antisense oligonucleotide (ASO) 14

<400>  18
gtagtatatc tggttccagc                                                   20


<210>  19
<211>  16
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  antisense oligonucleotide (ASO) 15

<400>  19
agaggtggcg tggtag                                                       16


<210>  20
<211>  169
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cy5-labeled IVT RNA

<400>  20
ccuugaaauc caugacgcag ggagaauugc gucauuuaaa gccuaguuaa cgcauuuacu       60

aaacgcagac gaaaauggaa agauuaauug ggagugguag gaugaaacaa uuuggagaag      120

auagaaguuu gaaguggaaa acuggaagac agaaguacgg gaaggcgaa                  169


<210>  21
<211>  16
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  SCR-ASOi

<400>  21
agagguggcg ugguag                                                       16


<210>  22
<211>  16
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Nucleoplasmin Nuclear Localization Signal

<400>  22

Lys Arg Pro Ala Ala Thr Lys Lys Ala Gly Gln Ala Lys Lys Lys Lys 
1               5                   10                  15      


<210>  23
<211>  278
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Bruton's Tyrosine Kinase (BTK) protein

<400>  23

Lys Asn Ala Pro Ser Thr Ala Gly Leu Gly Tyr Gly Ser Trp Glu Ile 
1               5                   10                  15      


Asp Pro Lys Asp Leu Thr Phe Leu Lys Glu Leu Gly Thr Gly Gln Phe 
            20                  25                  30          


Gly Val Val Lys Tyr Gly Lys Trp Arg Gly Gln Tyr Asp Val Ala Ile 
        35                  40                  45              


Lys Met Ile Lys Glu Gly Ser Met Ser Glu Asp Glu Phe Ile Glu Glu 
    50                  55                  60                  


Ala Lys Val Met Met Asn Leu Ser His Glu Lys Leu Val Gln Leu Tyr 
65                  70                  75                  80  


Gly Val Cys Thr Lys Gln Arg Pro Ile Phe Ile Ile Thr Glu Tyr Met 
                85                  90                  95      


Ala Asn Gly Cys Leu Leu Asn Tyr Leu Arg Glu Met Arg His Arg Phe 
            100                 105                 110         


Gln Thr Gln Gln Leu Leu Glu Met Cys Lys Asp Val Cys Glu Ala Met 
        115                 120                 125             


Glu Tyr Leu Glu Ser Lys Gln Phe Leu His Arg Asp Leu Ala Ala Arg 
    130                 135                 140                 


Asn Cys Leu Val Asn Asp Gln Gly Val Val Lys Val Ser Asp Phe Gly 
145                 150                 155                 160 


Leu Ser Arg Tyr Val Leu Asp Asp Glu Tyr Thr Ser Ser Val Gly Ser 
                165                 170                 175     


Lys Phe Pro Val Arg Trp Ser Pro Pro Glu Val Leu Met Tyr Ser Lys 
            180                 185                 190         


Phe Ser Ser Lys Ser Asp Ile Trp Ala Phe Gly Val Leu Met Trp Glu 
        195                 200                 205             


Ile Tyr Ser Leu Gly Lys Met Pro Tyr Glu Arg Phe Thr Asn Ser Glu 
    210                 215                 220                 


Thr Ala Glu His Ile Ala Gln Gly Leu Arg Leu Tyr Arg Pro His Leu 
225                 230                 235                 240 


Ala Ser Glu Lys Val Tyr Thr Ile Met Tyr Ser Cys Trp His Glu Lys 
                245                 250                 255     


Ala Asp Glu Arg Pro Thr Phe Lys Ile Leu Leu Ser Asn Ile Leu Asp 
            260                 265                 270         


Val Met Asp Glu Glu Ser 
        275             


<210>  24
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  SV40 Nuclear Localization Signal (NLS)

<400>  24

Pro Lys Lys Lys Arg Lys Val 
1               5           


<210>  25
<211>  238
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  monomeric enhanced green fluorescence protein (mEFGP)

<400>  25

Val Ser Lys Gly Glu Glu Leu Phe Thr Gly Val Val Pro Ile Leu Val 
1               5                   10                  15      


Glu Leu Asp Gly Asp Val Asn Gly His Lys Phe Ser Val Ser Gly Glu 
            20                  25                  30          


Gly Glu Gly Asp Ala Thr Tyr Gly Lys Leu Thr Leu Lys Phe Ile Cys 
        35                  40                  45              


Thr Thr Gly Lys Leu Pro Val Pro Trp Pro Thr Leu Val Thr Thr Leu 
    50                  55                  60                  


Thr Tyr Gly Val Gln Cys Phe Ser Arg Tyr Pro Asp His Met Lys Gln 
65                  70                  75                  80  


His Asp Phe Phe Lys Ser Ala Met Pro Glu Gly Tyr Val Gln Glu Arg 
                85                  90                  95      


Thr Ile Phe Phe Lys Asp Asp Gly Asn Tyr Lys Thr Arg Ala Glu Val 
            100                 105                 110         


Lys Phe Glu Gly Asp Thr Leu Val Asn Arg Ile Glu Leu Lys Gly Ile 
        115                 120                 125             


Asp Phe Lys Glu Asp Gly Asn Ile Leu Gly His Lys Leu Glu Tyr Asn 
    130                 135                 140                 


Tyr Asn Ser His Asn Val Tyr Ile Met Ala Asp Lys Gln Lys Asn Gly 
145                 150                 155                 160 


Ile Lys Val Asn Phe Lys Ile Arg His Asn Ile Glu Asp Gly Ser Val 
                165                 170                 175     


Gln Leu Ala Asp His Tyr Gln Gln Asn Thr Pro Ile Gly Asp Gly Pro 
            180                 185                 190         


Val Leu Leu Pro Asp Asn His Tyr Leu Ser Thr Gln Ser Lys Leu Ser 
        195                 200                 205             


Lys Asp Pro Asn Glu Lys Arg Asp His Met Val Leu Leu Glu Phe Val 
    210                 215                 220                 


Thr Ala Ala Gly Ile Thr Leu Gly Met Asp Glu Leu Tyr Lys 
225                 230                 235             


<210>  26
<211>  194
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  PIN domain

<400>  26

Met Glu Leu Glu Ile Arg Pro Leu Phe Leu Val Pro Asp Thr Asn Gly 
1               5                   10                  15      


Phe Ile Asp His Leu Ala Ser Leu Ala Arg Leu Leu Glu Ser Arg Lys 
            20                  25                  30          


Tyr Ile Leu Val Val Pro Leu Ile Val Ile Asn Glu Leu Asp Gly Leu 
        35                  40                  45              


Ala Lys Gly Gln Glu Thr Asp His Arg Ala Gly Gly Tyr Ala Arg Val 
    50                  55                  60                  


Val Gln Glu Lys Ala Arg Lys Ser Ile Glu Phe Leu Glu Gln Arg Phe 
65                  70                  75                  80  


Glu Ser Arg Asp Ser Cys Leu Arg Ala Leu Thr Ser Arg Gly Asn Glu 
                85                  90                  95      


Leu Glu Ser Ile Ala Phe Arg Ser Glu Asp Ile Thr Gly Gln Leu Gly 
            100                 105                 110         


Asn Asn Asp Asp Leu Ile Leu Ser Cys Cys Leu His Tyr Cys Lys Asp 
        115                 120                 125             


Lys Ala Lys Asp Phe Met Pro Ala Ser Lys Glu Glu Pro Ile Arg Leu 
    130                 135                 140                 


Leu Arg Glu Val Val Leu Leu Thr Asp Asp Arg Asn Leu Arg Val Lys 
145                 150                 155                 160 


Ala Leu Thr Arg Asn Val Pro Val Arg Asp Ile Pro Ala Phe Leu Thr 
                165                 170                 175     


Trp Ala Gln Val Gly Met Ser Ala Thr Arg Phe Arg Phe His Arg Arg 
            180                 185                 190         


Leu Leu 
        


<210>  27
<211>  16
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ASO targeting XIST

<400>  27

Gly Cys Gly Thr Ala Gly Ala Thr Gly Gly Gly Ala Thr Gly Gly Gly 
1               5                   10                  15      


<210>  28
<211>  16
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ASO targeting MALAT1

<400>  28

Cys Gly Thr Thr Ala Ala Cys Thr Ala Gly Gly Cys Thr Thr Thr Ala 
1               5                   10                  15      


<210>  29
<211>  20
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ASO targeting HSP70

<400>  29

Thr Cys Thr Thr Gly Gly Gly Cys Cys Gly Ala Gly Gly Cys Thr Ala 
1               5                   10                  15      


Cys Thr Gly Ala 
            20  


<210>  30
<211>  159
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  monomeric streptavidin (Mutein)

<400>  30

Met Asp Pro Ser Lys Asp Ser Lys Ala Gln Val Ser Ala Ala Glu Ala 
1               5                   10                  15      


Gly Ile Thr Gly Thr Trp Tyr Asn Gln Leu Gly Ser Thr Phe Ile Val 
            20                  25                  30          


Thr Ala Gly Ala Asp Gly Ala Leu Thr Gly Thr Tyr Glu Ser Ala Val 
        35                  40                  45              


Gly Asn Ala Glu Ser Arg Arg Leu Thr Gly Arg Tyr Asp Ser Ala Pro 
    50                  55                  60                  


Ala Thr Asp Gly Ser Gly Thr Ala Leu Gly Trp Arg Val Ala Trp Lys 
65                  70                  75                  80  


Asn Asn Tyr Arg Asn Ala His Ser Ala Thr Thr Trp Ser Gly Gln Tyr 
                85                  90                  95      


Val Gly Gly Ala Glu Ala Arg Ile Asn Thr Gln Trp Thr Leu Thr Ser 
            100                 105                 110         


Gly Thr Thr Glu Ala Asn Ala Trp Lys Ser Thr Leu Arg Gly His Asp 
        115                 120                 125             


Thr Phe Thr Lys Val Lys Pro Ser Ala Ala Ser Ile Asp Ala Ala Lys 
    130                 135                 140                 


Lys Ala Gly Val Asn Asn Gly Asn Pro Leu Asp Ala Val Gln Gln 
145                 150                 155                 


<210>  31
<211>  16
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Importin nuclear localization signal (NLS)

<400>  31

Lys Arg Pro Ala Ala Thr Lys Lys Ala Gly Gln Ala Lys Lys Lys Lys 
1               5                   10                  15      


<210>  32
<211>  226
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Turbo GFP protein

<400>  32

Met Ala Met Lys Ile Glu Cys Arg Ile Thr Gly Thr Leu Asn Gly Val 
1               5                   10                  15      


Glu Phe Glu Leu Val Gly Gly Gly Glu Gly Thr Pro Glu Gln Gly Arg 
            20                  25                  30          


Met Thr Asn Lys Met Lys Ser Thr Lys Gly Ala Leu Thr Phe Ser Pro 
        35                  40                  45              


Tyr Leu Leu Ser His Val Met Gly Tyr Gly Phe Tyr His Phe Gly Thr 
    50                  55                  60                  


Tyr Pro Ser Gly Tyr Glu Asn Pro Phe Leu His Ala Ile Asn Asn Gly 
65                  70                  75                  80  


Gly Tyr Thr Asn Thr Arg Ile Glu Lys Tyr Glu Asp Gly Gly Val Leu 
                85                  90                  95      


His Val Ser Phe Ser Tyr Arg Tyr Glu Ala Gly Arg Val Ile Gly Asp 
            100                 105                 110         


Phe Lys Val Val Gly Thr Gly Phe Pro Glu Asp Ser Val Ile Phe Thr 
        115                 120                 125             


Asp Lys Ile Ile Arg Ser Asn Ala Thr Val Glu His Leu His Pro Met 
    130                 135                 140                 


Gly Asp Asn Val Leu Val Gly Ser Phe Ala Arg Thr Phe Ser Leu Arg 
145                 150                 155                 160 


Asp Gly Gly Tyr Tyr Ser Phe Val Val Asp Ser His Met His Phe Lys 
                165                 170                 175     


Ser Ala Ile His Pro Ser Ile Leu Gln Asn Gly Gly Pro Met Phe Ala 
            180                 185                 190         


Phe Arg Arg Val Glu Glu Leu His Ser Asn Thr Glu Leu Gly Ile Val 
        195                 200                 205             


Glu Tyr Gln His Ala Phe Lys Thr Pro Ile Ala Phe Ala Arg Ser Arg 
    210                 215                 220                 


Ala Arg 
225     


<210>  33
<211>  1419
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  SMG6 protein

<400>  33

Met Ala Glu Gly Leu Glu Arg Val Arg Ile Ser Ala Ser Glu Leu Arg 
1               5                   10                  15      


Gly Ile Leu Ala Thr Leu Ala Pro Gln Ala Gly Ser Arg Glu Asn Met 
            20                  25                  30          


Lys Glu Leu Lys Glu Ala Arg Pro Arg Lys Asp Asn Arg Arg Pro Asp 
        35                  40                  45              


Leu Glu Ile Tyr Lys Pro Gly Leu Ser Arg Leu Arg Asn Lys Pro Lys 
    50                  55                  60                  


Ile Lys Glu Pro Pro Gly Ser Glu Glu Phe Lys Asp Glu Ile Val Asn 
65                  70                  75                  80  


Asp Arg Asp Cys Ser Ala Val Glu Asn Gly Thr Gln Pro Val Lys Asp 
                85                  90                  95      


Val Cys Lys Glu Leu Asn Asn Gln Glu Gln Asn Gly Pro Ile Asp Pro 
            100                 105                 110         


Glu Asn Asn Arg Gly Gln Glu Ser Phe Pro Arg Thr Ala Gly Gln Glu 
        115                 120                 125             


Asp Arg Ser Leu Lys Ile Ile Lys Arg Thr Lys Lys Pro Asp Leu Gln 
    130                 135                 140                 


Ile Tyr Gln Pro Gly Arg Arg Leu Gln Thr Val Ser Lys Glu Ser Ala 
145                 150                 155                 160 


Ser Arg Val Glu Glu Glu Glu Val Leu Asn Gln Val Glu Gln Leu Arg 
                165                 170                 175     


Val Glu Glu Asp Glu Cys Arg Gly Asn Val Ala Lys Glu Glu Val Ala 
            180                 185                 190         


Asn Lys Pro Asp Arg Ala Glu Ile Glu Lys Ser Pro Gly Gly Gly Arg 
        195                 200                 205             


Val Gly Ala Ala Lys Gly Glu Lys Gly Lys Arg Met Gly Lys Gly Glu 
    210                 215                 220                 


Gly Val Arg Glu Thr His Asp Asp Pro Ala Arg Gly Arg Pro Gly Ser 
225                 230                 235                 240 


Ala Lys Arg Tyr Ser Arg Ser Asp Lys Arg Arg Asn Arg Tyr Arg Thr 
                245                 250                 255     


Arg Ser Thr Ser Ser Ala Gly Ser Asn Asn Ser Ala Glu Gly Ala Gly 
            260                 265                 270         


Leu Thr Asp Asn Gly Cys Arg Arg Arg Arg Gln Asp Arg Thr Lys Glu 
        275                 280                 285             


Arg Pro Arg Leu Lys Lys Gln Val Ser Val Ser Ser Thr Asp Ser Leu 
    290                 295                 300                 


Asp Glu Asp Arg Ile Asp Glu Pro Asp Gly Leu Gly Pro Arg Arg Ser 
305                 310                 315                 320 


Ser Glu Arg Lys Arg His Leu Glu Arg Asn Trp Ser Gly Arg Gly Glu 
                325                 330                 335     


Gly Glu Gln Lys Asn Ser Ala Lys Glu Tyr Arg Gly Thr Leu Arg Val 
            340                 345                 350         


Thr Phe Asp Ala Glu Ala Met Asn Lys Glu Ser Pro Met Val Arg Ser 
        355                 360                 365             


Ala Arg Asp Asp Met Asp Arg Gly Lys Pro Asp Lys Gly Leu Ser Ser 
    370                 375                 380                 


Gly Gly Lys Gly Ser Glu Lys Gln Glu Ser Lys Asn Pro Lys Gln Glu 
385                 390                 395                 400 


Leu Arg Gly Arg Gly Arg Gly Ile Leu Ile Leu Pro Ala His Thr Thr 
                405                 410                 415     


Leu Ser Val Asn Ser Ala Gly Ser Pro Glu Ser Ala Pro Leu Gly Pro 
            420                 425                 430         


Arg Leu Leu Phe Gly Ser Gly Ser Lys Gly Ser Arg Ser Trp Gly Arg 
        435                 440                 445             


Gly Gly Thr Thr Arg Arg Leu Trp Asp Pro Asn Asn Pro Asp Gln Lys 
    450                 455                 460                 


Pro Ala Leu Lys Thr Gln Thr Pro Gln Leu His Phe Leu Asp Thr Asp 
465                 470                 475                 480 


Asp Glu Val Ser Pro Thr Ser Trp Gly Asp Ser Arg Gln Ala Gln Ala 
                485                 490                 495     


Ser Tyr Tyr Lys Phe Gln Asn Ser Asp Asn Pro Tyr Tyr Tyr Pro Arg 
            500                 505                 510         


Thr Pro Gly Pro Ala Ser Gln Tyr Pro Tyr Thr Gly Tyr Asn Pro Leu 
        515                 520                 525             


Gln Tyr Pro Val Gly Pro Thr Asn Gly Val Tyr Pro Gly Pro Tyr Tyr 
    530                 535                 540                 


Pro Gly Tyr Pro Thr Pro Ser Gly Gln Tyr Val Cys Ser Pro Leu Pro 
545                 550                 555                 560 


Thr Ser Thr Met Ser Pro Glu Glu Val Glu Gln His Met Arg Asn Leu 
                565                 570                 575     


Gln Gln Gln Glu Leu His Arg Leu Leu Arg Val Ala Asp Asn Gln Glu 
            580                 585                 590         


Leu Gln Leu Ser Asn Leu Leu Ser Arg Asp Arg Ile Ser Pro Glu Gly 
        595                 600                 605             


Leu Glu Lys Met Ala Gln Leu Arg Ala Glu Leu Leu Gln Leu Tyr Glu 
    610                 615                 620                 


Arg Cys Ile Leu Leu Asp Ile Glu Phe Ser Asp Asn Gln Asn Val Asp 
625                 630                 635                 640 


Gln Ile Leu Trp Lys Asn Ala Phe Tyr Gln Val Ile Glu Lys Phe Arg 
                645                 650                 655     


Gln Leu Val Lys Asp Pro Asn Val Glu Asn Pro Glu Gln Ile Arg Asn 
            660                 665                 670         


Arg Leu Leu Glu Leu Leu Asp Glu Gly Ser Asp Phe Phe Asp Ser Leu 
        675                 680                 685             


Leu Gln Lys Leu Gln Val Thr Tyr Lys Phe Lys Leu Glu Asp Tyr Met 
    690                 695                 700                 


Asp Gly Leu Ala Ile Arg Ser Lys Pro Leu Arg Lys Thr Val Lys Tyr 
705                 710                 715                 720 


Ala Leu Ile Ser Ala Gln Arg Cys Met Ile Cys Gln Gly Asp Ile Ala 
                725                 730                 735     


Arg Tyr Arg Glu Gln Ala Ser Asp Thr Ala Asn Tyr Gly Lys Ala Arg 
            740                 745                 750         


Ser Trp Tyr Leu Lys Ala Gln His Ile Ala Pro Lys Asn Gly Arg Pro 
        755                 760                 765             


Tyr Asn Gln Leu Ala Leu Leu Ala Val Tyr Thr Arg Arg Lys Leu Asp 
    770                 775                 780                 


Ala Val Tyr Tyr Tyr Met Arg Ser Leu Ala Ala Ser Asn Pro Ile Leu 
785                 790                 795                 800 


Thr Ala Lys Glu Ser Leu Met Ser Leu Phe Glu Glu Thr Lys Arg Lys 
                805                 810                 815     


Ala Glu Gln Met Glu Lys Lys Gln His Glu Glu Phe Asp Leu Ser Pro 
            820                 825                 830         


Asp Gln Trp Arg Lys Gly Lys Lys Ser Thr Phe Arg His Val Gly Asp 
        835                 840                 845             


Asp Thr Thr Arg Leu Glu Ile Trp Ile His Pro Ser His Pro Arg Ser 
    850                 855                 860                 


Ser Gln Gly Thr Glu Ser Gly Lys Asp Ser Glu Gln Glu Asn Gly Leu 
865                 870                 875                 880 


Gly Ser Leu Ser Pro Ser Asp Leu Asn Lys Arg Phe Ile Leu Ser Phe 
                885                 890                 895     


Leu His Ala His Gly Lys Leu Phe Thr Arg Ile Gly Met Glu Thr Phe 
            900                 905                 910         


Pro Ala Val Ala Glu Lys Val Leu Lys Glu Phe Gln Val Leu Leu Gln 
        915                 920                 925             


His Ser Pro Ser Pro Ile Gly Ser Thr Arg Met Leu Gln Leu Met Thr 
    930                 935                 940                 


Ile Asn Met Phe Ala Val His Asn Ser Gln Leu Lys Asp Cys Phe Ser 
945                 950                 955                 960 


Glu Glu Cys Arg Ser Val Ile Gln Glu Gln Ala Ala Ala Leu Gly Leu 
                965                 970                 975     


Ala Met Phe Ser Leu Leu Val Arg Arg Cys Thr Cys Leu Leu Lys Glu 
            980                 985                 990         


Ser Ala Lys Ala Gln Leu Ser Ser  Pro Glu Asp Gln Asp  Asp Gln Asp 
        995                 1000                 1005             


Asp Ile  Lys Val Ser Ser Phe  Val Pro Asp Leu Lys  Glu Leu Leu 
    1010                 1015                 1020             


Pro Ser  Val Lys Val Trp Ser  Asp Trp Met Leu Gly  Tyr Pro Asp 
    1025                 1030                 1035             


Thr Trp  Asn Pro Pro Pro Thr  Ser Leu Asp Leu Pro  Ser His Val 
    1040                 1045                 1050             


Ala Val  Asp Val Trp Ser Thr  Leu Ala Asp Phe Cys  Asn Ile Leu 
    1055                 1060                 1065             


Thr Ala  Val Asn Gln Ser Glu  Val Pro Leu Tyr Lys  Asp Pro Asp 
    1070                 1075                 1080             


Asp Asp  Leu Thr Leu Leu Ile  Leu Glu Glu Asp Arg  Leu Leu Ser 
    1085                 1090                 1095             


Gly Phe  Val Pro Leu Leu Ala  Ala Pro Gln Asp Pro  Cys Tyr Val 
    1100                 1105                 1110             


Glu Lys  Thr Ser Asp Lys Val  Ile Ala Ala Asp Cys  Lys Arg Val 
    1115                 1120                 1125             


Thr Val  Leu Lys Tyr Phe Leu  Glu Ala Leu Cys Gly  Gln Glu Glu 
    1130                 1135                 1140             


Pro Leu  Leu Ala Phe Lys Gly  Gly Lys Tyr Val Ser  Val Ala Pro 
    1145                 1150                 1155             


Val Pro  Asp Thr Met Gly Lys  Glu Met Gly Ser Gln  Glu Gly Thr 
    1160                 1165                 1170             


Arg Leu  Glu Asp Glu Glu Glu  Asp Val Val Ile Glu  Asp Phe Glu 
    1175                 1180                 1185             


Glu Asp  Ser Glu Ala Glu Gly  Ser Gly Gly Glu Asp  Asp Ile Arg 
    1190                 1195                 1200             


Glu Leu  Arg Ala Lys Lys Leu  Ala Leu Ala Arg Lys  Ile Ala Glu 
    1205                 1210                 1215             


Gln Gln  Arg Arg Gln Glu Lys  Ile Gln Ala Val Leu  Glu Asp His 
    1220                 1225                 1230             


Ser Gln  Met Arg Gln Met Glu  Leu Glu Ile Arg Pro  Leu Phe Leu 
    1235                 1240                 1245             


Val Pro  Asp Thr Asn Gly Phe  Ile Asp His Leu Ala  Ser Leu Ala 
    1250                 1255                 1260             


Arg Leu  Leu Glu Ser Arg Lys  Tyr Ile Leu Val Val  Pro Leu Ile 
    1265                 1270                 1275             


Val Ile  Asn Glu Leu Asp Gly  Leu Ala Lys Gly Gln  Glu Thr Asp 
    1280                 1285                 1290             


His Arg  Ala Gly Gly Tyr Ala  Arg Val Val Gln Glu  Lys Ala Arg 
    1295                 1300                 1305             


Lys Ser  Ile Glu Phe Leu Glu  Gln Arg Phe Glu Ser  Arg Asp Ser 
    1310                 1315                 1320             


Cys Leu  Arg Ala Leu Thr Ser  Arg Gly Asn Glu Leu  Glu Ser Ile 
    1325                 1330                 1335             


Ala Phe  Arg Ser Glu Asp Ile  Thr Gly Gln Leu Gly  Asn Asn Asp 
    1340                 1345                 1350             


Asp Leu  Ile Leu Ser Cys Cys  Leu His Tyr Cys Lys  Asp Lys Ala 
    1355                 1360                 1365             


Lys Asp  Phe Met Pro Ala Ser  Lys Glu Glu Pro Ile  Arg Leu Leu 
    1370                 1375                 1380             


Arg Glu  Val Val Leu Leu Thr  Asp Asp Arg Asn Leu  Arg Val Lys 
    1385                 1390                 1395             


Ala Leu  Thr Arg Asn Val Pro  Val Arg Asp Ile Pro  Ala Phe Leu 
    1400                 1405                 1410             


Thr Trp  Ala Gln Val Gly 
    1415                 


<210>  34
<211>  18
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  T2A self-cleaving peptide

<400>  34

Glu Gly Arg Gly Ser Leu Leu Thr Cys Gly Asp Val Glu Glu Asn Pro 
1               5                   10                  15      


Gly Pro 
        


<210>  35
<211>  238
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  monomeric enhanced fluorescence protein (mEGFP)

<400>  35

Val Ser Lys Gly Glu Glu Leu Phe Thr Gly Val Val Pro Ile Leu Val 
1               5                   10                  15      


Glu Leu Asp Gly Asp Val Asn Gly His Lys Phe Ser Val Ser Gly Glu 
            20                  25                  30          


Gly Glu Gly Asp Ala Thr Tyr Gly Lys Leu Thr Leu Lys Phe Ile Cys 
        35                  40                  45              


Thr Thr Gly Lys Leu Pro Val Pro Trp Pro Thr Leu Val Thr Thr Leu 
    50                  55                  60                  


Thr Tyr Gly Val Gln Cys Phe Ser Arg Tyr Pro Asp His Met Lys Gln 
65                  70                  75                  80  


His Asp Phe Phe Lys Ser Ala Met Pro Glu Gly Tyr Val Gln Glu Arg 
                85                  90                  95      


Thr Ile Phe Phe Lys Asp Asp Gly Asn Tyr Lys Thr Arg Ala Glu Val 
            100                 105                 110         


Lys Phe Glu Gly Asp Thr Leu Val Asn Arg Ile Glu Leu Lys Gly Ile 
        115                 120                 125             


Asp Phe Lys Glu Asp Gly Asn Ile Leu Gly His Lys Leu Glu Tyr Asn 
    130                 135                 140                 


Tyr Asn Ser His Asn Val Tyr Ile Met Ala Asp Lys Gln Lys Asn Gly 
145                 150                 155                 160 


Ile Lys Val Asn Phe Lys Ile Arg His Asn Ile Glu Asp Gly Ser Val 
                165                 170                 175     


Gln Leu Ala Asp His Tyr Gln Gln Asn Thr Pro Ile Gly Asp Gly Pro 
            180                 185                 190         


Val Leu Leu Pro Asp Asn His Tyr Leu Ser Thr Gln Ser Lys Leu Ser 
        195                 200                 205             


Lys Asp Pro Asn Glu Lys Arg Asp His Met Val Leu Leu Glu Phe Val 
    210                 215                 220                 


Thr Ala Ala Gly Ile Thr Leu Gly Met Asp Glu Leu Tyr Lys 
225                 230                 235             


<210>  36
<211>  307
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Sequence_CNOT7

<400>  36

Met Pro Ala Ala Thr Val Asp His Ser Gln Arg Ile Cys Glu Val Trp 
1               5                   10                  15      


Ala Cys Asn Leu Asp Glu Glu Met Lys Lys Ile Arg Gln Val Ile Arg 
            20                  25                  30          


Lys Tyr Asn Tyr Val Ala Met Asp Thr Glu Phe Pro Gly Val Val Ala 
        35                  40                  45              


Arg Pro Ile Gly Glu Phe Arg Ser Asn Ala Asp Tyr Gln Tyr Gln Leu 
    50                  55                  60                  


Leu Arg Cys Asn Val Asp Leu Leu Lys Ile Ile Gln Leu Gly Leu Thr 
65                  70                  75                  80  


Phe Met Asn Glu Gln Gly Glu Tyr Pro Pro Gly Thr Ser Thr Trp Gln 
                85                  90                  95      


Phe Asn Phe Lys Phe Asn Leu Thr Leu Gly Val Val Ala His Ala Cys 
            100                 105                 110         


Asn Pro Ser Thr Leu Gly Gly Arg Gly Gly Arg Ile Thr Arg Glu Asp 
        115                 120                 125             


Met Tyr Ala Gln Asp Ser Ile Glu Leu Leu Thr Thr Ser Gly Ile Gln 
    130                 135                 140                 


Phe Lys Lys His Glu Glu Glu Gly Ile Glu Thr Gln Tyr Phe Ala Glu 
145                 150                 155                 160 


Leu Leu Met Thr Ser Gly Val Val Leu Cys Glu Gly Val Lys Trp Leu 
                165                 170                 175     


Ser Phe His Ser Gly Tyr Asp Phe Gly Tyr Leu Ile Lys Ile Leu Thr 
            180                 185                 190         


Asn Ser Asn Leu Pro Glu Glu Glu Leu Asp Phe Phe Glu Ile Leu Arg 
        195                 200                 205             


Leu Phe Phe Pro Val Ile Tyr Asp Val Lys Tyr Leu Met Lys Ser Cys 
    210                 215                 220                 


Lys Asn Leu Lys Gly Gly Leu Gln Glu Val Ala Glu Gln Leu Glu Leu 
225                 230                 235                 240 


Glu Arg Ile Gly Pro Gln His Gln Ala Gly Ser Asp Ser Leu Leu Thr 
                245                 250                 255     


Gly Met Ala Phe Phe Lys Met Arg Glu Met Phe Phe Glu Asp His Ile 
            260                 265                 270         


Asp Asp Ala Lys Tyr Cys Gly His Leu Tyr Gly Leu Gly Ser Gly Ser 
        275                 280                 285             


Ser Tyr Val Gln Asn Gly Thr Gly Asn Ala Tyr Glu Glu Glu Ala Asn 
    290                 295                 300                 


Lys Gln Ser 
305         


<210>  37
<211>  1137
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  SMG7 protein

<400>  37

Met Ser Leu Gln Ser Ala Gln Tyr Leu Arg Gln Ala Glu Val Leu Lys 
1               5                   10                  15      


Ala Asp Met Thr Asp Ser Lys Leu Gly Pro Ala Glu Val Trp Thr Ser 
            20                  25                  30          


Arg Gln Ala Leu Gln Asp Leu Tyr Gln Lys Met Leu Val Thr Asp Leu 
        35                  40                  45              


Glu Tyr Ala Leu Asp Lys Lys Val Glu Gln Asp Leu Trp Asn His Ala 
    50                  55                  60                  


Phe Lys Asn Gln Ile Thr Thr Leu Gln Gly Gln Ala Lys Asn Arg Ala 
65                  70                  75                  80  


Asn Pro Asn Arg Ser Glu Val Gln Ala Asn Leu Ser Leu Phe Leu Glu 
                85                  90                  95      


Ala Ala Ser Gly Phe Tyr Thr Gln Leu Leu Gln Glu Leu Cys Thr Val 
            100                 105                 110         


Phe Asn Val Asp Leu Pro Cys Arg Val Lys Ser Ser Gln Leu Gly Ile 
        115                 120                 125             


Ile Ser Asn Lys Gln Thr His Thr Ser Ala Ile Val Lys Pro Gln Ser 
    130                 135                 140                 


Ser Ser Cys Ser Tyr Ile Cys Gln His Cys Leu Val His Leu Gly Asp 
145                 150                 155                 160 


Ile Ala Arg Tyr Arg Asn Gln Thr Ser Gln Ala Glu Ser Tyr Tyr Arg 
                165                 170                 175     


His Ala Ala Gln Leu Val Pro Ser Asn Gly Gln Pro Tyr Asn Gln Leu 
            180                 185                 190         


Ala Ile Leu Ala Ser Ser Lys Gly Asp His Leu Thr Thr Ile Phe Tyr 
        195                 200                 205             


Tyr Cys Arg Ser Ile Ala Val Lys Phe Pro Phe Pro Ala Ala Ser Thr 
    210                 215                 220                 


Asn Leu Gln Lys Ala Leu Ser Lys Ala Leu Glu Ser Arg Asp Glu Val 
225                 230                 235                 240 


Lys Thr Lys Trp Gly Val Ser Asp Phe Ile Lys Ala Phe Ile Lys Phe 
                245                 250                 255     


His Gly His Val Tyr Leu Ser Lys Ser Leu Glu Lys Leu Ser Pro Leu 
            260                 265                 270         


Arg Glu Lys Leu Glu Glu Gln Phe Lys Arg Leu Leu Phe Gln Lys Ala 
        275                 280                 285             


Phe Asn Ser Gln Gln Leu Val His Val Thr Val Ile Asn Leu Phe Gln 
    290                 295                 300                 


Leu His His Leu Arg Asp Phe Ser Asn Glu Thr Glu Gln His Thr Tyr 
305                 310                 315                 320 


Ser Gln Asp Glu Gln Leu Cys Trp Thr Gln Leu Leu Ala Leu Phe Met 
                325                 330                 335     


Ser Phe Leu Gly Ile Leu Cys Lys Cys Pro Leu Gln Asn Glu Ser Gln 
            340                 345                 350         


Glu Glu Ser Tyr Asn Ala Tyr Pro Leu Pro Ala Val Lys Val Ser Met 
        355                 360                 365             


Asp Trp Leu Arg Leu Arg Pro Arg Val Phe Gln Glu Ala Val Val Asp 
    370                 375                 380                 


Glu Arg Gln Tyr Ile Trp Pro Trp Leu Ile Ser Leu Leu Asn Ser Phe 
385                 390                 395                 400 


His Pro His Glu Glu Asp Leu Ser Ser Ile Ser Ala Thr Pro Leu Pro 
                405                 410                 415     


Glu Glu Phe Glu Leu Gln Gly Phe Leu Ala Leu Arg Pro Ser Phe Arg 
            420                 425                 430         


Asn Leu Asp Phe Ser Lys Gly His Gln Gly Ile Thr Gly Asp Lys Glu 
        435                 440                 445             


Gly Gln Gln Arg Arg Ile Arg Gln Gln Arg Leu Ile Ser Ile Gly Lys 
    450                 455                 460                 


Trp Ile Ala Asp Asn Gln Pro Arg Leu Ile Gln Cys Glu Asn Glu Val 
465                 470                 475                 480 


Gly Lys Leu Leu Phe Ile Thr Glu Ile Pro Glu Leu Ile Leu Glu Asp 
                485                 490                 495     


Pro Ser Glu Ala Lys Glu Asn Leu Ile Leu Gln Glu Thr Ser Val Ile 
            500                 505                 510         


Glu Ser Leu Ala Ala Asp Gly Ser Pro Gly Leu Lys Ser Val Leu Ser 
        515                 520                 525             


Thr Ser Arg Asn Leu Ser Asn Asn Cys Asp Thr Gly Glu Lys Pro Val 
    530                 535                 540                 


Val Thr Phe Lys Glu Asn Ile Lys Thr Arg Glu Val Asn Arg Asp Gln 
545                 550                 555                 560 


Gly Arg Ser Phe Pro Pro Lys Glu Val Arg Arg Asp Tyr Ser Lys Gly 
                565                 570                 575     


Ile Thr Val Thr Lys Asn Asp Gly Lys Lys Asp Asn Asn Lys Arg Lys 
            580                 585                 590         


Thr Glu Thr Lys Lys Cys Thr Leu Glu Lys Leu Gln Glu Thr Gly Lys 
        595                 600                 605             


Gln Asn Val Ala Val Gln Val Lys Ser Gln Thr Glu Leu Arg Lys Thr 
    610                 615                 620                 


Pro Val Ser Glu Ala Arg Lys Thr Pro Val Thr Gln Thr Pro Thr Gln 
625                 630                 635                 640 


Ala Ser Asn Ser Gln Phe Ile Pro Ile His His Pro Gly Ala Phe Pro 
                645                 650                 655     


Pro Leu Pro Ser Arg Pro Gly Phe Pro Pro Pro Thr Tyr Val Ile Pro 
            660                 665                 670         


Pro Pro Val Ala Phe Ser Met Gly Ser Gly Tyr Thr Phe Pro Ala Gly 
        675                 680                 685             


Val Ser Val Pro Gly Thr Phe Leu Gln Pro Thr Ala His Ser Pro Ala 
    690                 695                 700                 


Gly Asn Gln Val Gln Ala Gly Lys Gln Ser His Ile Pro Tyr Ser Gln 
705                 710                 715                 720 


Gln Arg Pro Ser Gly Pro Gly Pro Met Asn Gln Gly Pro Gln Gln Ser 
                725                 730                 735     


Gln Pro Pro Ser Gln Gln Pro Leu Thr Ser Leu Pro Ala Gln Pro Thr 
            740                 745                 750         


Ala Gln Ser Thr Ser Gln Leu Gln Val Gln Ala Leu Thr Gln Gln Gln 
        755                 760                 765             


Gln Ser Pro Thr Lys Ala Val Pro Ala Leu Gly Lys Ser Pro Pro His 
    770                 775                 780                 


His Ser Gly Phe Gln Gln Tyr Gln Gln Ala Asp Ala Ser Lys Gln Leu 
785                 790                 795                 800 


Trp Asn Pro Pro Gln Val Gln Gly Pro Leu Gly Lys Ile Met Pro Val 
                805                 810                 815     


Lys Gln Pro Tyr Tyr Leu Gln Thr Gln Asp Pro Ile Lys Leu Phe Glu 
            820                 825                 830         


Pro Ser Leu Gln Pro Pro Val Met Gln Gln Gln Pro Leu Glu Lys Lys 
        835                 840                 845             


Met Lys Pro Phe Pro Met Glu Pro Tyr Asn His Asn Pro Ser Glu Val 
    850                 855                 860                 


Lys Val Pro Glu Phe Tyr Trp Asp Ser Ser Tyr Ser Met Ala Asp Asn 
865                 870                 875                 880 


Arg Ser Val Met Ala Gln Gln Ala Asn Ile Asp Arg Arg Gly Lys Arg 
                885                 890                 895     


Ser Pro Gly Val Phe Arg Pro Glu Gln Asp Pro Val Pro Arg Met Pro 
            900                 905                 910         


Phe Glu Lys Ser Leu Leu Glu Lys Pro Ser Glu Leu Met Ser His Ser 
        915                 920                 925             


Ser Ser Phe Leu Ser Leu Thr Gly Phe Ser Leu Asn Gln Glu Arg Tyr 
    930                 935                 940                 


Pro Asn Asn Ser Met Phe Asn Glu Val Tyr Gly Lys Asn Leu Thr Ser 
945                 950                 955                 960 


Ser Ser Lys Ala Glu Leu Ser Pro Ser Met Ala Pro Gln Glu Thr Ser 
                965                 970                 975     


Leu Tyr Ser Leu Phe Glu Gly Thr Pro Trp Ser Pro Ser Leu Pro Ala 
            980                 985                 990         


Ser Ser Asp His Ser Thr Pro Ala  Ser Gln Ser Pro His  Ser Ser Asn 
        995                 1000                 1005             


Pro Ser  Ser Leu Pro Ser Ser  Pro Pro Thr His Asn  His Asn Ser 
    1010                 1015                 1020             


Val Pro  Phe Ser Asn Phe Gly  Pro Ile Gly Thr Pro  Asp Asn Arg 
    1025                 1030                 1035             


Asp Arg  Arg Thr Ala Asp Arg  Trp Lys Thr Asp Lys  Pro Ala Met 
    1040                 1045                 1050             


Gly Gly  Phe Gly Ile Asp Tyr  Leu Ser Ala Thr Ser  Ser Ser Glu 
    1055                 1060                 1065             


Ser Ser  Trp His Gln Ala Ser  Thr Pro Ser Gly Thr  Trp Thr Gly 
    1070                 1075                 1080             


His Gly  Pro Ser Met Glu Asp  Ser Ser Ala Val Leu  Met Glu Ser 
    1085                 1090                 1095             


Leu Lys  Ser Ile Trp Ser Ser  Ser Met Met His Pro  Gly Pro Ser 
    1100                 1105                 1110             


Ala Leu  Glu Gln Leu Leu Met  Gln Gln Lys Gln Lys  Gln Gln Arg 
    1115                 1120                 1125             


Gly Gln  Gly Thr Met Asn Pro  Pro His 
    1130                 1135         


