                         SEQUENCE LISTING

<110>  University of Pittsburgh - Of the Commonwealth System of 
       Higher Education
 
<120>  NON-HUMAN ANIMAL SECRETOME MODELS

<130>  48881-0005WO1

<140>  PCT/US2021/028340
<141>  2021-04-21

<150>  63/013,453
<151>  2020-04-21

<160>  16    

<170>  PatentIn version 3.5

<210>  1
<211>  6
<212>  PRT
<213>  artificial

<220>
<223>  retention sequence

<400>  1

Lys Asp Glu Leu Glu Arg 
1               5       


<210>  2
<211>  233
<212>  PRT
<213>  artificial

<220>
<223>  biotin ligase

<400>  2

Met Phe Lys Asn Leu Ile Trp Leu Lys Glu Val Asp Ser Thr Gln Glu 
1               5                   10                  15      


Arg Leu Lys Glu Trp Asn Val Ser Tyr Gly Thr Ala Leu Val Ala Asp 
            20                  25                  30          


Arg Gln Thr Lys Gly Arg Gly Gly Leu Gly Arg Lys Trp Leu Ser Gln 
        35                  40                  45              


Glu Gly Gly Leu Tyr Phe Ser Phe Leu Leu Asn Pro Lys Glu Phe Glu 
    50                  55                  60                  


Asn Leu Leu Gln Leu Pro Leu Val Leu Gly Leu Ser Val Ser Glu Ala 
65                  70                  75                  80  


Leu Glu Glu Ile Thr Glu Ile Pro Phe Ser Leu Lys Trp Pro Asn Asp 
                85                  90                  95      


Val Tyr Phe Gln Glu Lys Lys Val Ser Gly Val Leu Cys Glu Leu Ser 
            100                 105                 110         


Lys Asp Lys Leu Ile Val Gly Ile Gly Ile Asn Val Asn Gln Arg Glu 
        115                 120                 125             


Ile Pro Glu Glu Ile Lys Asp Arg Ala Thr Thr Leu Tyr Glu Ile Thr 
    130                 135                 140                 


Gly Lys Asp Trp Asp Arg Lys Glu Val Leu Leu Lys Val Leu Lys Arg 
145                 150                 155                 160 


Ile Ser Glu Asn Leu Lys Lys Phe Lys Glu Lys Ser Phe Lys Glu Phe 
                165                 170                 175     


Lys Gly Lys Ile Glu Ser Lys Met Leu Tyr Leu Gly Glu Glu Val Lys 
            180                 185                 190         


Leu Leu Gly Glu Gly Lys Ile Thr Gly Lys Leu Val Gly Leu Ser Glu 
        195                 200                 205             


Lys Gly Gly Ala Leu Ile Leu Thr Glu Glu Gly Ile Lys Glu Ile Leu 
    210                 215                 220                 


Ser Gly Glu Phe Ser Leu Arg Arg Ser 
225                 230             


<210>  3
<211>  701
<212>  DNA
<213>  artificial

<220>
<223>  nucleic acid encoding a biotin ligase

<400>  3
atgttcaaga acctgatctg gctgaaggag gtggacagca cccaggagag actgaaggag       60

tggaacgtta gctacggcac cgccctggtg gccgacagac agaccaaggg cagaggcggc      120

ctgggcagaa agtggctgag ccaggagggc ggcctgtact tcagcttcct gctgaacccc      180

aaggagttcg agaacctgct gcagctgccc ctggtgctgg gcctgagcgt gagcgaggcc      240

ctggaggaga tcaccgagat ccccttcagc ctgaagtggc ccaacgacgt gtacttccag      300

gagaagaagg tgagcggcgt gctgtgcgag ctgagcaagg acaagctgat cgtgggcatc      360

ggcatcaacg tgaaccagag agagatcccc gaggagatca aggacagagc caccaccctg      420

tacgagatca ccggcaagga ctgggacaga aaggaggtgc tgctgaaggt gctgaagaga      480

atcagcgaga acctgaagaa gttcaaggag aagagcttca aggagttcaa gggcaagatc      540

gagagcaaga tgctgtacct gggcgaggag gtgaagctgc tgggcgaggg caagatcacc      600

ggcaagctgg tgggcctgag cgagaagggc ggcgccctga tcctgaccga ggagggcatc      660

aaggagatcc tgagcggcga gttcagcctg agaagaagct g                          701


<210>  4
<211>  236
<212>  PRT
<213>  artificial

<220>
<223>  polypeptide that includes an IgK signal peptide, a biotin ligase,
       an HA tag, and an ER retention signal

<400>  4

Met Glu Thr Asp Thr Leu Leu Leu Trp Val Leu Leu Leu Trp Val Pro 
1               5                   10                  15      


Gly Ser Thr Gly Asp Phe Lys Asn Leu Ile Trp Leu Lys Glu Val Asp 
            20                  25                  30          


Ser Thr Gln Glu Arg Leu Lys Glu Trp Asn Val Ser Tyr Gly Thr Ala 
        35                  40                  45              


Leu Val Ala Asp Arg Gln Thr Lys Gly Arg Gly Gly Leu Gly Arg Lys 
    50                  55                  60                  


Trp Leu Ser Gln Glu Gly Gly Leu Tyr Phe Ser Phe Leu Leu Asn Pro 
65                  70                  75                  80  


Lys Glu Phe Glu Asn Leu Leu Gln Leu Pro Leu Val Leu Gly Leu Ser 
                85                  90                  95      


Val Ser Glu Ala Leu Glu Glu Ile Thr Glu Ile Pro Phe Ser Leu Lys 
            100                 105                 110         


Trp Pro Asn Asp Val Tyr Phe Gln Glu Lys Lys Val Ser Gly Val Leu 
        115                 120                 125             


Cys Glu Leu Ser Lys Asp Lys Leu Ile Val Gly Ile Gly Ile Asn Val 
    130                 135                 140                 


Asn Gln Arg Glu Ile Pro Glu Glu Ile Lys Asp Arg Ala Thr Thr Leu 
145                 150                 155                 160 


Tyr Glu Ile Thr Gly Lys Asp Trp Asp Arg Lys Glu Val Leu Leu Lys 
                165                 170                 175     


Val Leu Lys Arg Ile Ser Glu Asn Leu Lys Lys Phe Lys Glu Lys Ser 
            180                 185                 190         


Phe Lys Glu Phe Lys Gly Lys Ile Glu Ser Lys Met Leu Tyr Leu Gly 
        195                 200                 205             


Glu Glu Val Lys Leu Leu Gly Glu Gly Lys Ile Thr Gly Lys Leu Val 
    210                 215                 220                 


Gly Leu Ser Glu Lys Gly Gly Ala Leu Ile Leu Thr 
225                 230                 235     


<210>  5
<211>  801
<212>  DNA
<213>  artificial

<220>
<223>  nucleic acid encoding a polypeptide that includes an IgK signal 
       peptide, a biotin ligase, an HA tag, and an ER retention signal

<400>  5
atggagacag acacactcct gctatgggta ctgctgctct gggttccagg ttccactggt       60

gacttcaaga acctgatctg gctgaaggag gtggacagca cccaggagag actgaaggag      120

tggaacgtta gctacggcac cgccctggtg gccgacagac agaccaaggg cagaggcggc      180

ctgggcagaa agtggctgag ccaggagggc ggcctgtact tcagcttcct gctgaacccc      240

aaggagttcg agaacctgct gcagctgccc ctggtgctgg gcctgagcgt gagcgaggcc      300

ctggaggaga tcaccgagat ccccttcagc ctgaagtggc ccaacgacgt gtacttccag      360

gagaagaagg tgagcggcgt gctgtgcgag ctgagcaagg acaagctgat cgtgggcatc      420

ggcatcaacg tgaaccagag agagatcccc gaggagatca aggacagagc caccaccctg      480

tacgagatca ccggcaagga ctgggacaga aaggaggtgc tgctgaaggt gctgaagaga      540

atcagcgaga acctgaagaa gttcaaggag aagagcttca aggagttcaa gggcaagatc      600

gagagcaaga tgctgtacct gggcgaggag gtgaagctgc tgggcgaggg caagatcacc      660

ggcaagctgg tgggcctgag cgagaagggc ggcgccctga tcctgaccga ggagggcatc      720

aaggagatcc tgagcggcga gttcagcctg agaagaagct acccttacga tgtaccggat      780

tacgcaaagg acgagctgtg a                                                801


<210>  6
<211>  4
<212>  PRT
<213>  artificial

<220>
<223>  ER retention sequence

<400>  6

Ser Asp Glu Leu 
1               


<210>  7
<211>  4
<212>  PRT
<213>  artificial

<220>
<223>  ER retention sequence

<400>  7

Tyr Glu Glu Leu 
1               


<210>  8
<211>  4
<212>  PRT
<213>  artificial

<220>
<223>  ER retention sequence

<400>  8

Arg Asp Glu Leu 
1               


<210>  9
<211>  21
<212>  DNA
<213>  artificial

<220>
<223>  primer

<400>  9
ctagagcctc tgctaaccat g                                                 21


<210>  10
<211>  26
<212>  DNA
<213>  artificial

<220>
<223>  primer

<400>  10
cagatcaggt tcttgaagtc accagt                                            26


<210>  11
<211>  20
<212>  DNA
<213>  artificial

<220>
<223>  primer

<400>  11
taagtctgaa cccggtctgc                                                   20


<210>  12
<211>  23
<212>  DNA
<213>  artificial

<220>
<223>  primer

<400>  12
gtgaaacagc attgctgtca ctt                                               23


<210>  13
<211>  23
<212>  DNA
<213>  artificial

<220>
<223>  primer

<400>  13
gcggtctggc agtaaaaact atc                                               23


<210>  14
<211>  23
<212>  DNA
<213>  artificial

<220>
<223>  primer

<400>  14
gtgaaacagc attgctgtca ctt                                               23


<210>  15
<211>  100
<212>  DNA
<213>  artificial

<220>
<223>  primer

<400>  15
tccgctagca tggagacaga cacactcctg ctatgggtac tgctgctctg ggttccaggt       60

tccactggtg acttcaagaa cctgatctgg ctgaaggagg                            100


<210>  16
<211>  56
<212>  DNA
<213>  artificial

<220>
<223>  primer

<400>  16
acttgtacac tatcacagct cgtcctttgc gtaatccggt acatcgtaag ggtagc           56


