                         SEQUENCE LISTING

<110>  AbSci LLC
 
<120>  POLYPEPTIDE CLEAVAGE METHODS

<130>  AbSci-009P1/10294-105926-01

<160>  124   

<170>  PatentIn version 3.5

<210>  1
<211>  151
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synechocystis sp. PCC6803 DnaX 6xHis-tagged non-ligating 
       mini-intein

<400>  1

Met Ala Leu Thr Gly Asp Ser Gln Val Leu Thr Arg Asn Gly Leu Met 
1               5                   10                  15      


Ser Ile Asp Asn Pro Gln Ile Lys Gly Arg Glu Val Leu Ser Tyr Asn 
            20                  25                  30          


Glu Thr Leu Gln Gln Trp Glu Tyr Lys Lys Val Leu Arg Trp Leu Asp 
        35                  40                  45              


Arg Gly Glu Lys Gln Thr Leu Ser Ile Lys Thr Lys Asn Ser Thr Val 
    50                  55                  60                  


Arg Cys Thr Ala Asn His Leu Ile Arg Thr Glu Gln Gly Trp Thr Arg 
65                  70                  75                  80  


Ala Glu Asn Ile Thr Pro Gly Met Lys Ile Leu Ser Pro Ala Ser Ala 
                85                  90                  95      


Ser Gly His His His His His His Gly Gly Ser Gly Ser Ser Pro Gln 
            100                 105                 110         


Trp His Thr Asn Phe Glu Glu Val Glu Ser Val Thr Lys Gly Gln Val 
        115                 120                 125             


Glu Lys Val Tyr Asp Leu Glu Val Glu Asp Asn His Asn Phe Val Ala 
    130                 135                 140                 


Asn Gly Leu Leu Val His Asn 
145                 150     


<210>  2
<211>  169
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synechocystis sp. PCC6803 DnaB 6xHis-tagged non-ligating 
       mini-intein

<400>  2

Met Ala Ile Ser Gly Asp Ser Leu Ile Ser Leu Ala Ser Thr Gly Lys 
1               5                   10                  15      


Arg Val Ser Ile Lys Asp Leu Leu Asp Glu Lys Asp Phe Glu Ile Trp 
            20                  25                  30          


Ala Ile Asn Glu Gln Thr Met Lys Leu Glu Ser Ala Lys Val Ser Arg 
        35                  40                  45              


Val Phe Cys Thr Gly Lys Lys Leu Val Tyr Ile Leu Lys Thr Arg Leu 
    50                  55                  60                  


Gly Arg Thr Ile Lys Ala Thr Ala Asn His Arg Phe Leu Thr Ile Asp 
65                  70                  75                  80  


Gly Trp Lys Arg Leu Asp Glu Leu Ser Leu Lys Glu His Ile Ala Leu 
                85                  90                  95      


Pro Arg Lys Leu Glu Ser Ser Ser Leu Gln Leu Ala Ser Gly His His 
            100                 105                 110         


His His His His Gly Gly Ser Gly Ser Ser Pro Glu Ile Glu Lys Leu 
        115                 120                 125             


Ser Gln Ser Asp Ile Tyr Trp Asp Ser Ile Val Ser Ile Thr Glu Thr 
    130                 135                 140                 


Gly Val Glu Glu Val Phe Asp Leu Thr Val Pro Gly Pro His Asn Phe 
145                 150                 155                 160 


Val Ala Asn Asp Ile Ile Val His Asn 
                165                 


<210>  3
<211>  140
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Desulfofundulus thermosubterraneus DSM 16057 DnaB non-ligating 
       intein

<400>  3

Met Ala Leu Ala Gly Glu Thr Leu Val Gln Leu Ala Ser Gly His Arg 
1               5                   10                  15      


Val Pro Ile Lys Asp Leu Val Arg Glu Lys Gln Pro Val Lys Val Met 
            20                  25                  30          


Ala Leu Asn Glu Ile Thr Trp Lys Leu Glu Pro Ala Ala Val Ser Lys 
        35                  40                  45              


Val Trp Cys Thr Gly Val Lys Pro Val Phe Arg Leu Cys Thr Gln Leu 
    50                  55                  60                  


Gly Arg Glu Val Arg Ala Thr Ala Asn His Lys Phe Arg Thr Leu Glu 
65                  70                  75                  80  


Gly Trp Lys Cys Leu Gly Glu Leu Gln Pro Gly Glu Phe Ile Ala Leu 
                85                  90                  95      


Pro Arg Ser Leu Glu Ser Asp Val Phe Trp Asp Arg Val Val Ser Ile 
            100                 105                 110         


Glu Pro Asp Gly Ile Glu Glu Val Tyr Asp Leu Glu Val Pro Gly His 
        115                 120                 125             


His Asn Phe Val Ala Ala Asp Ile Ile Val His Asn 
    130                 135                 140 


<210>  4
<211>  136
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Prochlorococcus cyanophage P-SSM2 gp41-1 6xHis-tagged 
       non-ligating mini-intein

<400>  4

Met Ala Leu Asp Leu Lys Thr Gln Val Gln Thr Pro Gln Gly Met Lys 
1               5                   10                  15      


Glu Ile Ser Asn Ile Gln Val Gly Asp Leu Val Leu Ser Asn Thr Gly 
            20                  25                  30          


Tyr Asn Glu Val Leu Asn Val Phe Pro Lys Ser Lys Lys Lys Ser Tyr 
        35                  40                  45              


Lys Ile Thr Leu Glu Asp Gly Lys Glu Ile Ile Cys Ser Glu Glu His 
    50                  55                  60                  


Leu Phe Pro Thr Gln Thr Gly Glu Met Asn Ile Ser Gly Gly Leu Lys 
65                  70                  75                  80  


Glu Gly Met Cys Leu Tyr Val Lys Glu Gly His His His His His His 
                85                  90                  95      


Gly Gly Ser Gly Met Leu Lys Lys Ile Leu Lys Ile Glu Glu Leu Asp 
            100                 105                 110         


Glu Arg Glu Leu Ile Asp Ile Glu Val Ser Gly Asn His Leu Phe Tyr 
        115                 120                 125             


Ala Asn Asp Ile Leu Thr His Asn 
    130                 135     


<210>  5
<211>  140
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Nostoc punctiforme strain ATCC 29133 / PCC 73102 DnaB 
       non-ligating mini-intein

<400>  5

Met Ala Leu Ala Gly Asp Ser Leu Val Thr Leu Val Asp Ser Gly Leu 
1               5                   10                  15      


Gln Val Pro Ile Lys Glu Leu Val Gly Lys Ser Gly Phe Ala Val Trp 
            20                  25                  30          


Ala Leu Asn Glu Ala Thr Met Gln Leu Glu Lys Ala Ile Val Ser Asn 
        35                  40                  45              


Ala Phe Ser Thr Gly Ile Lys Pro Leu Phe Thr Leu Thr Thr Arg Leu 
    50                  55                  60                  


Gly Arg Lys Ile Arg Ala Thr Gly Asn His Lys Phe Leu Thr Ile Asn 
65                  70                  75                  80  


Gly Trp Lys Arg Leu Asp Glu Leu Thr Pro Lys Glu His Leu Ala Leu 
                85                  90                  95      


Pro Arg Asn Ser Gly Ser Asp Ile Tyr Trp Asp Glu Ile Val Ser Ile 
            100                 105                 110         


Thr Tyr Ser Gly Glu Glu Glu Val Phe Asp Leu Thr Val Pro Gly Leu 
        115                 120                 125             


His Asn Phe Val Ala Asn Asn Ile Ile Val His Asn 
    130                 135                 140 


<210>  6
<211>  170
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Mycobacterium tuberculosis strain ATCC 25618 / H37Rv RecA 
       non-ligating mini-intein

<400>  6

Met Ala Leu Ala Glu Gly Thr Arg Ile Phe Asp Pro Val Thr Gly Thr 
1               5                   10                  15      


Thr His Arg Ile Glu Asp Val Val Gly Gly Arg Lys Pro Ile His Val 
            20                  25                  30          


Val Ala Ala Ala Lys Asp Gly Thr Leu His Ala Arg Pro Val Val Ser 
        35                  40                  45              


Trp Phe Asp Gln Gly Thr Arg Asp Val Ile Gly Leu Arg Ile Ala Gly 
    50                  55                  60                  


Gly Ala Ile Leu Trp Ala Thr Pro Asp His Lys Val Leu Thr Glu Tyr 
65                  70                  75                  80  


Gly Trp Arg Ala Ala Gly Glu Leu Arg Lys Gly Asp Arg Val Ala Gln 
                85                  90                  95      


Pro Arg Arg Phe Asp Gly Phe Gly Asp Ser Ala Pro Ile Pro Ala Asp 
            100                 105                 110         


Arg Val Gln Ala Leu Ala Asp Ala Leu Asp Asp Lys Phe Leu His Asp 
        115                 120                 125             


Met Leu Ala Glu Glu Leu Arg Tyr Ser Val Ile Arg Glu Val Leu Pro 
    130                 135                 140                 


Thr Arg Arg Ala Arg Thr Phe Gly Leu Glu Val Glu Glu Leu His Thr 
145                 150                 155                 160 


Leu Val Ala Glu Gly Val Val Val His Asn 
                165                 170 


<210>  7
<211>  136
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Clostridium thermocellum CthBIL4 N115D non-ligating intein

<400>  7

Met Ala Phe Val Ala Gly Thr Met Ile Leu Thr Ala Thr Gly Leu Val 
1               5                   10                  15      


Ala Ile Glu Asn Ile Lys Ala Gly Asp Lys Val Ile Ala Thr Asn Pro 
            20                  25                  30          


Glu Thr Phe Glu Val Ala Glu Lys Thr Val Leu Glu Thr Tyr Val Arg 
        35                  40                  45              


Glu Thr Thr Glu Leu Leu His Leu Thr Ile Gly Gly Glu Val Ile Lys 
    50                  55                  60                  


Thr Thr Phe Asp His Pro Phe Tyr Val Lys Asp Val Gly Phe Val Glu 
65                  70                  75                  80  


Ala Gly Lys Leu Gln Val Gly Asp Lys Leu Leu Asp Ser Arg Gly Asn 
                85                  90                  95      


Val Leu Val Val Glu Glu Lys Lys Leu Glu Ile Ala Asp Lys Pro Val 
            100                 105                 110         


Lys Val Tyr Asp Phe Lys Val Asp Asp Phe His Thr Tyr His Val Gly 
        115                 120                 125             


Asp Asn Glu Val Leu Val His Asn 
    130                 135     


<210>  8
<211>  168
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Deinococcus swuensis PolIII subunit alpha non-ligating 
       mini-intein

<400>  8

Met Ala Leu Thr Gly Asp Thr Arg Val Pro Val Ala Gly Gly Glu Leu 
1               5                   10                  15      


Arg Arg Ile Glu Asp Leu Cys Gly Glu Gly Gln Pro Val Gln Leu Pro 
            20                  25                  30          


Ser Val Asn Gly Glu Tyr Arg Leu Glu Leu Arg Pro Thr Gly Gln Phe 
        35                  40                  45              


Phe Asp Asn Gly Val Lys Pro Val Phe Lys Val Lys Thr Ala Leu Gly 
    50                  55                  60                  


Arg Glu Leu Thr Ala Thr Gly Asn His Pro Leu Leu Thr Leu Asp Gly 
65                  70                  75                  80  


Trp Trp Arg Asp Val Glu Ala Gln Ser Gly Val Cys Thr Lys Glu Phe 
                85                  90                  95      


Tyr Gly Thr Pro Lys Ala His Lys Lys Gly Phe Arg Arg Ser Thr Ile 
            100                 105                 110         


Gln Thr Leu Gly Glu Phe Phe Gly Ala Thr Glu Leu Leu Asp Ala Cys 
        115                 120                 125             


Leu Asp Asp Leu Tyr Trp Asp Thr Ile Thr Ser Ile Thr Pro Ala Gly 
    130                 135                 140                 


Glu Ala Gln Thr Phe Asp Leu Glu Val Pro Gly Thr His Asn Phe Val 
145                 150                 155                 160 


Ala Asn Asp Leu Ile Val His Asn 
                165             


<210>  9
<211>  163
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cyanobacterium aponinum DnaB non-ligating intein

<400>  9

Met Ala Leu Ser Gly Asp Ser Leu Ile Ala Leu Ala Asp Thr Asn Thr 
1               5                   10                  15      


Val Val Pro Ile Arg Glu Leu Val Ser Lys Lys Asp Ile Thr Val Phe 
            20                  25                  30          


Ala Leu Asn Glu Ala Thr Met Lys Ile Glu Lys Ala Lys Val Ile Asn 
        35                  40                  45              


Ala Phe Cys Thr Gly Ile Lys Pro Val Phe Cys Leu Lys Thr Arg Leu 
    50                  55                  60                  


Gly Arg Thr Ile Lys Ala Thr Ala Asn His Lys Phe Phe Thr Ile Glu 
65                  70                  75                  80  


Gly Trp Lys Arg Leu Asp Glu Leu Ser Val Lys Asp Phe Ile Ala Leu 
                85                  90                  95      


Pro Arg Tyr Leu Pro Ser Ser Glu Ile Ser Thr Ile Lys Leu Gly Glu 
            100                 105                 110         


Ile Val Gln Cys Asp Val Ile Lys Ser Leu Ala Asn Ser Asp Ile Tyr 
        115                 120                 125             


Trp Asp Glu Ile Thr Ser Ile Glu Pro Leu Gly Glu Thr Glu Val Tyr 
    130                 135                 140                 


Asp Leu Thr Val Glu Asn Tyr His Asn Phe Ile Ala Asn Asn Ile Ile 
145                 150                 155                 160 


Val His Asn 
            


<210>  10
<211>  95
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synechocystis sp. PCC6803 DnaX non-ligating split intein, 
       N-terminal portion

<400>  10

Met Ala Leu Thr Gly Asp Ser Gln Val Leu Thr Arg Asn Gly Leu Met 
1               5                   10                  15      


Ser Ile Asp Asn Pro Gln Ile Lys Gly Arg Glu Val Leu Ser Tyr Asn 
            20                  25                  30          


Glu Thr Leu Gln Gln Trp Glu Tyr Lys Lys Val Leu Arg Trp Leu Asp 
        35                  40                  45              


Arg Gly Glu Lys Gln Thr Leu Ser Ile Lys Thr Lys Asn Ser Thr Val 
    50                  55                  60                  


Arg Cys Thr Ala Asn His Leu Ile Arg Thr Glu Gln Gly Trp Thr Arg 
65                  70                  75                  80  


Ala Glu Asn Ile Thr Pro Gly Met Lys Ile Leu Ser Pro Ala Ser 
                85                  90                  95  


<210>  11
<211>  43
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synechocystis sp. PCC6803 DnaX non-ligating split intein, 
       C-terminal portion

<400>  11

Met Ser Pro Gln Trp His Thr Asn Phe Glu Glu Val Glu Ser Val Thr 
1               5                   10                  15      


Lys Gly Gln Val Glu Lys Val Tyr Asp Leu Glu Val Glu Asp Asn His 
            20                  25                  30          


Asn Phe Val Ala Asn Gly Leu Leu Val His Asn 
        35                  40              


<210>  12
<211>  61
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synechocystis sp. PCC6803 DnaX 6xHis-tagged non-ligating split 
       intein, C-terminal portion

<400>  12

Met His His His His His His Asp Tyr Lys Asp Asp Asp Asp Lys Gly 
1               5                   10                  15      


Ser Gly Ser Ser Pro Gln Trp His Thr Asn Phe Glu Glu Val Glu Ser 
            20                  25                  30          


Val Thr Lys Gly Gln Val Glu Lys Val Tyr Asp Leu Glu Val Glu Asp 
        35                  40                  45              


Asn His Asn Phe Val Ala Asn Gly Leu Leu Val His Asn 
    50                  55                  60      


<210>  13
<211>  107
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synechocystis sp. PCC6803 DnaB non-ligating split intein, 
       N-terminal portion

<400>  13

Met Ala Ile Ser Gly Asp Ser Leu Ile Ser Leu Ala Ser Thr Gly Lys 
1               5                   10                  15      


Arg Val Ser Ile Lys Asp Leu Leu Asp Glu Lys Asp Phe Glu Ile Trp 
            20                  25                  30          


Ala Ile Asn Glu Gln Thr Met Lys Leu Glu Ser Ala Lys Val Ser Arg 
        35                  40                  45              


Val Phe Cys Thr Gly Lys Lys Leu Val Tyr Ile Leu Lys Thr Arg Leu 
    50                  55                  60                  


Gly Arg Thr Ile Lys Ala Thr Ala Asn His Arg Phe Leu Thr Ile Asp 
65                  70                  75                  80  


Gly Trp Lys Arg Leu Asp Glu Leu Ser Leu Lys Glu His Ile Ala Leu 
                85                  90                  95      


Pro Arg Lys Leu Glu Ser Ser Ser Leu Gln Leu 
            100                 105         


<210>  14
<211>  49
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synechocystis sp. PCC6803 DnaB non-ligating split intein, 
       C-terminal portion

<400>  14

Met Ser Pro Glu Ile Glu Lys Leu Ser Gln Ser Asp Ile Tyr Trp Asp 
1               5                   10                  15      


Ser Ile Val Ser Ile Thr Glu Thr Gly Val Glu Glu Val Phe Asp Leu 
            20                  25                  30          


Thr Val Pro Gly Pro His Asn Phe Val Ala Asn Asp Ile Ile Val His 
        35                  40                  45              


Asn 
    


<210>  15
<211>  67
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synechocystis sp. PCC6803 DnaB 6xHis-tagged non-ligating split 
       intein, C-terminal portion

<400>  15

Met His His His His His His Asp Tyr Lys Asp Asp Asp Asp Lys Gly 
1               5                   10                  15      


Ser Gly Ser Ser Pro Glu Ile Glu Lys Leu Ser Gln Ser Asp Ile Tyr 
            20                  25                  30          


Trp Asp Ser Ile Val Ser Ile Thr Glu Thr Gly Val Glu Glu Val Phe 
        35                  40                  45              


Asp Leu Thr Val Pro Gly Pro His Asn Phe Val Ala Asn Asp Ile Ile 
    50                  55                  60                  


Val His Asn 
65          


<210>  16
<211>  89
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Prochlorococcus cyanophage P-SSM2 gp41-1 non-ligating split 
       intein, N-terminal portion

<400>  16

Met Ala Leu Asp Leu Lys Thr Gln Val Gln Thr Pro Gln Gly Met Lys 
1               5                   10                  15      


Glu Ile Ser Asn Ile Gln Val Gly Asp Leu Val Leu Ser Asn Thr Gly 
            20                  25                  30          


Tyr Asn Glu Val Leu Asn Val Phe Pro Lys Ser Lys Lys Lys Ser Tyr 
        35                  40                  45              


Lys Ile Thr Leu Glu Asp Gly Lys Glu Ile Ile Cys Ser Glu Glu His 
    50                  55                  60                  


Leu Phe Pro Thr Gln Thr Gly Glu Met Asn Ile Ser Gly Gly Leu Lys 
65                  70                  75                  80  


Glu Gly Met Cys Leu Tyr Val Lys Glu 
                85                  


<210>  17
<211>  36
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Prochlorococcus cyanophage P-SSM2 gp41-1 non-ligating split 
       intein, C-terminal portion

<400>  17

Met Leu Lys Lys Ile Leu Lys Ile Glu Glu Leu Asp Glu Arg Glu Leu 
1               5                   10                  15      


Ile Asp Ile Glu Val Ser Gly Asn His Leu Phe Tyr Ala Asn Asp Ile 
            20                  25                  30          


Leu Thr His Asn 
        35      


<210>  18
<211>  94
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Clostridium thermocellum CthBIL4 N115D non-ligating split intein 
       'C42', N-terminal portion

<400>  18

Met Ala Phe Val Ala Gly Thr Met Ile Leu Thr Ala Thr Gly Leu Val 
1               5                   10                  15      


Ala Ile Glu Asn Ile Lys Ala Gly Asp Lys Val Ile Ala Thr Asn Pro 
            20                  25                  30          


Glu Thr Phe Glu Val Ala Glu Lys Thr Val Leu Glu Thr Tyr Val Arg 
        35                  40                  45              


Glu Thr Thr Glu Leu Leu His Leu Thr Ile Gly Gly Glu Val Ile Lys 
    50                  55                  60                  


Thr Thr Phe Asp His Pro Phe Tyr Val Lys Asp Val Gly Phe Val Glu 
65                  70                  75                  80  


Ala Gly Lys Leu Gln Val Gly Asp Lys Leu Leu Asp Ser Arg 
                85                  90                  


<210>  19
<211>  43
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Clostridium thermocellum CthBIL4 N115D non-ligating split intein 
       'C42', C-terminal portion

<400>  19

Met Gly Asn Val Leu Val Val Glu Glu Lys Lys Leu Glu Ile Ala Asp 
1               5                   10                  15      


Lys Pro Val Lys Val Tyr Asp Phe Lys Val Asp Asp Phe His Thr Tyr 
            20                  25                  30          


His Val Gly Asp Asn Glu Val Leu Val His Asn 
        35                  40              


<210>  20
<211>  120
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Clostridium thermocellum CthBIL4 N115D non-ligating split intein 
       'C16', N-terminal portion

<400>  20

Met Ala Phe Val Ala Gly Thr Met Ile Leu Thr Ala Thr Gly Leu Val 
1               5                   10                  15      


Ala Ile Glu Asn Ile Lys Ala Gly Asp Lys Val Ile Ala Thr Asn Pro 
            20                  25                  30          


Glu Thr Phe Glu Val Ala Glu Lys Thr Val Leu Glu Thr Tyr Val Arg 
        35                  40                  45              


Glu Thr Thr Glu Leu Leu His Leu Thr Ile Gly Gly Glu Val Ile Lys 
    50                  55                  60                  


Thr Thr Phe Asp His Pro Phe Tyr Val Lys Asp Val Gly Phe Val Glu 
65                  70                  75                  80  


Ala Gly Lys Leu Gln Val Gly Asp Lys Leu Leu Asp Ser Arg Gly Asn 
                85                  90                  95      


Val Leu Val Val Glu Glu Lys Lys Leu Glu Ile Ala Asp Lys Pro Val 
            100                 105                 110         


Lys Val Tyr Asp Phe Lys Val Asp 
        115                 120 


<210>  21
<211>  17
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Clostridium thermocellum CthBIL4 N115D non-ligating split intein 
       'C16', C-terminal portion

<400>  21

Met Asp Phe His Thr Tyr His Val Gly Asp Asn Glu Val Leu Val His 
1               5                   10                  15      


Asn 
    


<210>  22
<211>  227
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  TRAST-Fab heavy chain

<400>  22

Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Asn Ile Lys Asp Thr 
            20                  25                  30          


Tyr Ile His Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val 
        35                  40                  45              


Ala Arg Ile Tyr Pro Thr Asn Gly Tyr Thr Arg Tyr Ala Asp Ser Val 
    50                  55                  60                  


Lys Gly Arg Phe Thr Ile Ser Ala Asp Thr Ser Lys Asn Thr Ala Tyr 
65                  70                  75                  80  


Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ser Arg Trp Gly Gly Asp Gly Phe Tyr Ala Met Asp Tyr Trp Gly Gln 
            100                 105                 110         


Gly Thr Leu Val Thr Val Ser Ser Ala Ser Thr Lys Gly Pro Ser Val 
        115                 120                 125             


Phe Pro Leu Ala Pro Ser Ser Lys Ser Thr Ser Gly Gly Thr Ala Ala 
    130                 135                 140                 


Leu Gly Cys Leu Val Lys Asp Tyr Phe Pro Glu Pro Val Thr Val Ser 
145                 150                 155                 160 


Trp Asn Ser Gly Ala Leu Thr Ser Gly Val His Thr Phe Pro Ala Val 
                165                 170                 175     


Leu Gln Ser Ser Gly Leu Tyr Ser Leu Ser Ser Val Val Thr Val Pro 
            180                 185                 190         


Ser Ser Ser Leu Gly Thr Gln Thr Tyr Ile Cys Asn Val Asn His Lys 
        195                 200                 205             


Pro Ser Asn Thr Lys Val Asp Lys Lys Val Glu Pro Lys Ser Cys Asp 
    210                 215                 220                 


Lys Thr His 
225         


<210>  23
<211>  214
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  TRAST-Fab light chain

<400>  23

Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly 
1               5                   10                  15      


Asp Arg Val Thr Ile Thr Cys Arg Ala Ser Gln Asp Val Asn Thr Ala 
            20                  25                  30          


Val Ala Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Leu Leu Ile 
        35                  40                  45              


Tyr Ser Ala Ser Phe Leu Tyr Ser Gly Val Pro Ser Arg Phe Ser Gly 
    50                  55                  60                  


Ser Arg Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu Gln Pro 
65                  70                  75                  80  


Glu Asp Phe Ala Thr Tyr Tyr Cys Gln Gln His Tyr Thr Thr Pro Pro 
                85                  90                  95      


Thr Phe Gly Gln Gly Thr Lys Val Glu Ile Lys Arg Thr Val Ala Ala 
            100                 105                 110         


Pro Ser Val Phe Ile Phe Pro Pro Ser Asp Glu Gln Leu Lys Ser Gly 
        115                 120                 125             


Thr Ala Ser Val Val Cys Leu Leu Asn Asn Phe Tyr Pro Arg Glu Ala 
    130                 135                 140                 


Lys Val Gln Trp Lys Val Asp Asn Ala Leu Gln Ser Gly Asn Ser Gln 
145                 150                 155                 160 


Glu Ser Val Thr Glu Gln Asp Ser Lys Asp Ser Thr Tyr Ser Leu Ser 
                165                 170                 175     


Ser Thr Leu Thr Leu Ser Lys Ala Asp Tyr Glu Lys His Lys Val Tyr 
            180                 185                 190         


Ala Cys Glu Val Thr His Gln Gly Leu Ser Ser Pro Val Thr Lys Ser 
        195                 200                 205             


Phe Asn Arg Gly Glu Cys 
    210                 


<210>  24
<211>  453
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Codon-optimized sequence #1 encoding Synechocystis sp. PCC6803 
       DnaX 6xHis-tagged non-ligating mini-intein (SEQ ID NO:1)

<400>  24
atggctctta ccggagactc gcaggtcctt acacgcaacg ggttaatgtc tattgacaac       60

ccacagatta agggtcgtga ggtactttcg tacaatgaaa cacttcaaca gtgggaatat      120

aagaaagtct tacgttggtt ggatcgcggc gagaagcaaa ctctttcaat caaaacaaaa      180

aactcgacgg ttcgctgtac agccaaccac ttaattcgta ccgaacaggg gtggacacgt      240

gcggaaaata tcacgccggg catgaaaatt ctttcccctg ccagcgcaag tggtcatcat      300

caccatcatc atgggggtag cggatcatcg ccacaatggc ataccaattt tgaggaggtg      360

gagtcggtta caaaaggtca ggtggaaaag gtatatgacc ttgaggtaga agacaatcac      420

aattttgttg caaatgggct tttagttcat aac                                   453


<210>  25
<211>  453
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Codon-optimized sequence #2 encoding Synechocystis sp. PCC6803 
       DnaX 6xHis-tagged non-ligating mini-intein (SEQ ID NO:1)

<400>  25
atggccttaa caggggactc ccaagtgttg acccgtaatg gtttaatgag tatcgataac       60

ccgcagatca agggtcgtga agtattatca tataatgaaa cgctgcaaca gtgggaatac      120

aaaaaggttt tacgttggct ggaccgtggg gagaagcaga cactgtcgat caagacgaaa      180

aactccaccg tccgctgcac tgcaaaccac cttatccgca ccgaacaagg ctggacccgc      240

gcagagaaca tcacacctgg aatgaaaatc cttagtcctg cgtctgcctc aggacaccat      300

caccatcatc atgggggaag tgggtccagt ccgcagtggc atactaattt cgaggaggta      360

gagtcagtca ctaagggtca ggtggagaag gtttatgatt tggaagtgga agacaatcat      420

aatttcgtgg caaatggtct gcttgttcat aac                                   453


<210>  26
<211>  507
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Codon-optimized sequence #1 encoding Synechocystis sp. PCC6803 
       DnaB 6xHis-tagged non-ligating mini-intein (SEQ ID NO:2)

<400>  26
atggctattt caggcgacag tctgatctct cttgcttcca cagggaagcg cgtatctatt       60

aaagatttgt tggacgaaaa agattttgaa atttgggcta tcaacgagca aaccatgaag      120

ctggaatctg caaaggtcag ccgcgtcttc tgcacaggta aaaaacttgt ttacattctt      180

aaaacgcgtc tgggtcgtac aatcaaggcg acagccaacc atcgcttttt aaccattgat      240

ggttggaagc gtctggatga gttgtctctg aaggagcaca tcgcgcttcc tcgtaaactg      300

gagtcgtcta gtttgcaatt ggcctccggg caccatcacc atcatcatgg ggggtcgggg      360

tcttcgccgg agatcgaaaa gttgtcccag tccgacatct actgggattc gatcgttagc      420

atcactgaga caggagtgga agaggtgttc gacttaacgg tgcctggacc ccataacttt      480

gtggcgaacg atatcatcgt tcacaac                                          507


<210>  27
<211>  507
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Codon-optimized sequence #2 encoding Synechocystis sp. PCC6803 
       DnaB 6xHis-tagged non-ligating mini-intein (SEQ ID NO:2)

<400>  27
atggccatta gcggagactc cctgatctct ttggcttcta ccggaaagcg cgtaagcatc       60

aaagacctgc ttgatgagaa agatttcgaa atctgggcga ttaacgagca aactatgaaa      120

ttggagtctg ctaaggtaag tcgcgtattc tgcactggga aaaagttagt ttatattttg      180

aaaacacgtt tagggcgcac tattaaggca acagcaaatc accgctttct gaccattgat      240

ggttggaaac gtctggatga gcttagtctt aaagaacaca tcgctttgcc tcgcaaactt      300

gaatcgtcat cgcttcaact ggcgtcaggc catcatcatc accatcatgg gggatcgggt      360

tcatctcccg agattgagaa actttcccag agcgacatct attgggatag tatcgtgagt      420

attactgaaa cgggggtgga ggaggtgttc gacttgaccg tccctggtcc tcataacttt      480

gttgcgaacg atattattgt tcacaac                                          507


<210>  28
<211>  420
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Codon-optimized sequence #1 encoding Desulfofundulus 
       thermosubterraneus DSM 16057 DnaB non-ligating intein (SEQ ID 
       NO:3)

<400>  28
atggcattag ccggtgaaac cctggtacaa ctggcctccg gtcaccgtgt cccaatcaaa       60

gaccttgtac gtgagaaaca gcccgttaag gttatggcac tgaatgaaat cacatggaaa      120

cttgaacctg ctgccgtctc taaggtttgg tgcactggtg taaaacccgt attccgctta      180

tgcacgcaac ttggccgtga ggtacgcgcg acagctaatc ataaattccg caccttagaa      240

ggttggaagt gtcttggaga gttgcaaccg ggagagttta tcgccttgcc tcgtagtctg      300

gagagcgatg tgttctggga ccgcgtcgtc tcaatcgaac cggatgggat cgaggaagtc      360

tatgatctgg aagtccctgg acatcacaat tttgtagccg ccgatattat cgtccataat      420


<210>  29
<211>  420
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Codon-optimized sequence #2 encoding Desulfofundulus 
       thermosubterraneus DSM 16057 DnaB non-ligating intein (SEQ ID 
       NO:3)

<400>  29
atggcgctgg caggcgaaac attagtccaa cttgcatctg gtcaccgcgt gccaatcaag       60

gatcttgtgc gcgagaaaca accagttaag gtcatggcac ttaacgagat cacttggaaa      120

ctggaacctg cggctgtctc caaagtttgg tgtacggggg ttaagccggt gttccgcttg      180

tgcacgcaac tggggcgtga agtacgtgcg acggcaaatc ataagtttcg cacattagaa      240

ggttggaaat gccttggtga attacaacct ggagagttta ttgccttgcc gcgctcactg      300

gagagcgatg ttttttggga ccgtgttgtc tccattgaac cggatggtat tgaggaagtt      360

tacgaccttg aggttccagg ccaccacaac tttgtagcag ccgacattat tgtccataat      420


<210>  30
<211>  408
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Codon-optimized sequence #1 encoding Prochlorococcus cyanophage 
       P-SSM2 gp41-1 6xHis-tagged non-ligating mini-intein (SEQ ID NO:4)

<400>  30
atggccctgg atctgaagac gcaagttcag acgccgcagg gtatgaagga aatctcgaac       60

attcaagtcg gtgatttagt gcttagcaat acgggctaca acgaagtcct gaacgtattt      120

cctaaatcaa aaaagaaatc ctacaagatt actttagaag acgggaagga gatcatttgt      180

agtgaagagc acttgtttcc tacccaaacc ggagagatga atatcagcgg tggtcttaag      240

gaaggtatgt gtttatacgt gaaagaggga caccatcatc atcaccacgg tgggagtggt      300

atgcttaaga aaatcttgaa gattgaggag ttagatgagc gcgaactgat tgacattgag      360

gtgtcaggaa accatctgtt ctatgccaac gacattctta cgcacaac                   408


<210>  31
<211>  408
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Codon-optimized sequence #2 encoding Prochlorococcus cyanophage 
       P-SSM2 gp41-1 6xHis-tagged non-ligating mini-intein (SEQ ID NO:4)

<400>  31
atggccctgg acttaaagac acaagtgcag accccacaag gtatgaaaga gatcagtaac       60

atccaagtcg gagacttagt gctgagtaat acgggataca atgaggtgct gaatgttttc      120

ccgaagtcta agaagaaaag ttataaaatt acgctggagg atggcaagga gattatttgc      180

tcagaggaac atcttttccc cacacaaacg ggcgagatga acatttcggg cggccttaaa      240

gagggaatgt gcttatatgt caaagaggga catcaccatc atcatcatgg aggttctggc      300

atgttgaaaa agattttgaa gatcgaagag ttggacgaac gcgaattgat cgacatcgag      360

gtatcaggca accatttatt ttatgcgaat gacatcttaa cacacaac                   408


<210>  32
<211>  420
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Codon-optimized sequence #1 encoding Nostoc punctiforme strain 
       ATCC 29133 / PCC 73102 DnaB non-ligating mini-intein (SEQ ID 
       NO:5)

<400>  32
atggccctgg ccggagacag tttggtaaca ttagttgatt cggggctgca agtcccaatc       60

aaggaactgg tcggcaagtc aggatttgca gtatgggcct taaacgaagc gaccatgcag      120

ttggagaaag cgattgtaag taatgctttc tcaacaggta tcaagccatt attcaccctt      180

accacccgct taggccgcaa gatccgtgca acaggaaatc ataaatttct gaccattaac      240

ggttggaagc gtctggacga gttgacaccc aaagagcatc ttgcgttgcc tcgcaactct      300

gggagcgaca tttactggga tgagatcgtt agtattacct actcaggtga agaggaagtg      360

ttcgatttga ccgttccggg tcttcataat tttgtagcta ataatattat cgttcataac      420


<210>  33
<211>  420
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Codon-optimized sequence #2 encoding Nostoc punctiforme strain 
       ATCC 29133 / PCC 73102 DnaB non-ligating mini-intein (SEQ ID 
       NO:5)

<400>  33
atggctttag cgggagactc tctggttact ctggttgaca gcgggttaca ggttccaatt       60

aaagaacttg ttggaaagtc gggattcgct gtttgggcac tgaacgaggc tactatgcag      120

cttgagaaag caatcgtctc aaacgcgttc tctacgggta ttaagcccct ttttactctt      180

acgacccgct tgggccgcaa gatccgtgcg actggtaacc ataaattcct taccatcaac      240

ggctggaagc gcctggatga gctgactcct aaggagcatt tagctcttcc acgcaattca      300

ggatctgata tttactggga tgaaattgtg tctatcacgt attctggcga agaggaggta      360

ttcgacctga ctgtgccggg tttgcataac ttcgtcgcaa acaacattat tgtccataac      420


<210>  34
<211>  510
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Codon-optimized sequence #1 encoding Mycobacterium tuberculosis 
       strain ATCC 25618 / H37Rv RecA non-ligating mini-intein (SEQ ID 
       NO:6)

<400>  34
atggccttgg ctgaagggac acgtatcttc gatccagtta cgggtacaac gcaccgcatc       60

gaagatgtag tgggtggtcg taagccgatt catgtagttg ctgctgcgaa agacgggact      120

ttgcacgcgc gtcctgtagt ttcctggttc gatcagggaa ctcgtgacgt tattggtctt      180

cgcattgcag ggggtgccat tttatgggcc acgccagatc acaaagttct taccgagtac      240

gggtggcgtg cagcaggcga acttcgtaaa ggggatcgcg tggcacagcc tcgccgcttt      300

gatgggtttg gtgattctgc tcccattcca gcagatcgtg tacaagcgtt ggctgatgcc      360

ctggacgaca aattcttaca tgatatgctt gctgaggagt tgcgctattc agttattcgc      420

gaagttttac ctactcgccg cgcccgcacg ttcggtctgg aagtggagga actgcacact      480

ttagtagctg aaggagtagt agtgcacaat                                       510


<210>  35
<211>  510
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Codon-optimized sequence #2 encoding Mycobacterium tuberculosis 
       strain ATCC 25618 / H37Rv RecA non-ligating mini-intein (SEQ ID 
       NO:6)

<400>  35
atggcccttg cagaaggtac acgcattttt gaccccgtca ctggcaccac ccaccgcatc       60

gaggacgtgg taggcgggcg caaaccgatc catgtagtag ctgcggctaa agatggaact      120

ttgcacgctc gtccggtagt ctcgtggttt gatcagggga cacgcgacgt tatcggatta      180

cgtatcgcgg gtggggctat tctttgggcc acaccagacc acaaagtact taccgagtat      240

ggttggcgtg cagccggcga actgcgtaaa ggtgatcgtg ttgcacaacc ccgtcgcttt      300

gacggattcg gggactctgc gccgatccct gctgatcgcg tgcaggcact tgccgatgct      360

cttgatgata aattcttgca cgacatgtta gcggaagaac ttcgttacag tgtaattcgt      420

gaggtattac cgacccgccg cgctcgcaca tttggattag aagtggagga gctgcataca      480

ctggtcgctg aaggtgtcgt tgttcataat                                       510


<210>  36
<211>  408
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Codon-optimized sequence #1 encoding Clostridium thermocellum 
       CthBIL4 N115D engineered BIL (bacterial non-ligating intein-like)
       (SEQ ID NO:7)

<400>  36
atggcttttg tagcgggcac catgattctt acagctaccg gcttagtcgc catcgaaaat       60

atcaaggcag gcgacaaggt aatcgcgaca aatcctgaga cttttgaggt cgccgagaag      120

actgtattgg aaacctatgt ccgtgagaca actgaattgc tgcacctgac gattggtggt      180

gaagtgatta aaaccacttt cgatcacccc ttctacgtaa aggatgtagg ttttgtggag      240

gccggtaaat tacaggttgg ggacaagctg ttagattcgc gcggtaacgt tctggtggtc      300

gaagaaaaga aacttgagat tgcagacaaa cccgtgaaag tctacgactt caaggtggac      360

gactttcaca cttaccacgt cggagataat gaagtgcttg tccataac                   408


<210>  37
<211>  408
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Codon-optimized sequence #2 encoding Clostridium thermocellum 
       CthBIL4 N115D engineered BIL (bacterial non-ligating intein-like)
       (SEQ ID NO:7)

<400>  37
atggcgtttg tagcaggcac gatgattctt acggctaccg gtctggtggc aattgaaaac       60

atcaaggctg gggacaaggt gatcgccaca aatcccgaaa cgtttgaggt cgccgaaaaa      120

accgtgttag agacttatgt tcgtgaaacc acagaacttt tgcatttgac gattggcgga      180

gaagtcatca aaacgacttt cgaccatcct ttctatgtga aagatgtcgg tttcgtggaa      240

gcaggtaagt tacaggtggg cgacaagtta ttagattcgc gcgggaatgt tttagtcgta      300

gaggagaaaa agttggagat tgccgacaag cctgttaaag tctatgactt caaagtggat      360

gacttccaca cctaccatgt gggagataac gaagtgttag ttcacaat                   408


<210>  38
<211>  504
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Codon-optimized sequence #1 encoding Deinococcus swuensis PolIII 
       alpha subunit non-ligating mini-intein (SEQ ID NO:8)

<400>  38
atggcgctga ccggggatac tcgcgtgcct gttgcaggcg gagaactgcg ccgtatcgag       60

gatttatgtg gtgaaggcca acctgttcaa ttaccgtcgg tgaatggaga ataccgtctt      120

gagctgcgcc cgactgggca gttttttgac aacggcgtga agccggtctt taaggtaaag      180

accgcgcttg gtcgtgaatt aaccgctacg gggaaccacc cacttttaac gttggacggt      240

tggtggcgcg acgtcgaagc gcaatccggg gtttgtacca aggagttcta tgggacccct      300

aaagcgcata agaaggggtt tcgccgttct acgatccaaa cgcttggtga gttcttcggg      360

gcgactgaac tgctggatgc ctgccttgat gatttatatt gggacacaat cacgtccatc      420

acaccagcgg gcgaggcaca aactttcgat ttggaagtgc ctggtacaca caacttcgta      480

gccaatgatc tgatcgtaca taac                                             504


<210>  39
<211>  504
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Codon-optimized sequence #2 encoding Deinococcus swuensis PolIII 
       alpha subunit non-ligating mini-intein (SEQ ID NO:8)

<400>  39
atggccttga cgggagatac ccgtgttccc gttgccgggg gagaattacg tcgtattgaa       60

gatctttgcg gggaagggca gccagttcaa ttgcctagtg tgaacggcga atatcgttta      120

gaactgcgtc ctacaggtca gttctttgat aatggtgtga aacccgtgtt taaggtaaag      180

actgcattag gacgcgagtt gactgccacc gggaatcacc cgcttcttac ccttgatggg      240

tggtggcgcg atgtggaagc ccaatcaggc gtatgtacga aagagttcta cggcacgccc      300

aaggcacaca aaaaaggctt ccgtcgctcc acaattcaaa cattaggcga attttttgga      360

gcaaccgaac tgttagatgc ctgtttagat gatctgtact gggatacaat cacaagcatt      420

acacctgccg gagaagccca aacgttcgac ttagaggtcc caggcactca caattttgta      480

gcaaacgacc ttatcgtaca caat                                             504


<210>  40
<211>  489
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Codon-optimized sequence #1 encoding Cyanobacterium aponinum DnaB
       non-ligating intein (SEQ ID NO:9)

<400>  40
atggcgctgt ccggggattc cttaattgca ctggctgata ctaacacggt ggtgccaatt       60

cgtgaattag tgagcaagaa ggacatcaca gtgttcgcgt taaatgaggc gacaatgaag      120

atcgaaaaag ctaaagtcat caatgccttc tgcacgggca tcaagccagt attttgtctt      180

aagacacgtt taggtcgtac tatcaaggcc accgccaatc acaaattctt cactatcgag      240

ggttggaaac gtcttgacga attatccgtt aaagatttca tcgcgcttcc tcgctattta      300

ccctcctcgg aaatctctac aattaaactg ggagaaattg tgcaatgtga cgtaattaag      360

tcgttagcta actcggatat ttattgggat gaaatcacct caatcgaacc attaggcgag      420

acggaagtgt acgaccttac tgtagagaac tatcataatt tcattgccaa caatatcatt      480

gttcacaac                                                              489


<210>  41
<211>  489
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Codon-optimized sequence #2 encoding Cyanobacterium aponinum DnaB
       non-ligating intein (SEQ ID NO:9)

<400>  41
atggcattaa gcggggacag tctgattgct ttggcggaca caaatacagt tgtgcctatt       60

cgtgagcttg tgagtaaaaa agacatcacg gtttttgctt tgaacgaagc tactatgaaa      120

attgagaaag ccaaggtgat taatgctttc tgtacgggga ttaagcccgt cttttgcctt      180

aaaacccgcc tgggacgcac catcaaggcc accgcaaatc acaaattttt tactattgaa      240

gggtggaagc gtttagatga acttagcgtt aaggacttta ttgcgctgcc gcgctactta      300

ccctcatcag aaatctcgac gattaagtta ggtgaaatcg tgcagtgcga tgttatcaaa      360

tctttagcaa acagtgacat ttactgggat gagattacta gcatcgaacc cttaggcgaa      420

acagaagtat acgatttgac tgtggaaaat taccacaatt tcatcgccaa taacatcatt      480

gtccacaac                                                              489


<210>  42
<211>  285
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Codon-optimized sequence encoding Synechocystis sp. PCC6803 DnaX 
       non-ligating split intein, N-terminal portion (SEQ ID NO:10)

<400>  42
atggctctta ccggagactc gcaggtcctt acacgcaacg ggttaatgtc tattgacaac       60

ccacagatta agggtcgtga ggtactttcg tacaatgaaa cacttcaaca gtgggaatat      120

aagaaagtct tacgttggtt ggatcgcggc gagaagcaaa ctctttcaat caaaacaaaa      180

aactcgacgg ttcgctgtac agccaaccac ttaattcgta ccgaacaggg gtggacacgt      240

gcggaaaata tcacgccggg catgaaaatt ctttcccctg ccagc                      285


<210>  43
<211>  129
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Codon-optimized sequence #1 encoding Synechocystis sp. PCC6803 
       DnaX non-ligating split intein, C-terminal portion (SEQ ID NO:11)

<400>  43
atgtcgccac aatggcatac caattttgag gaggtggagt cggttacaaa aggtcaggtg       60

gaaaaggtat atgaccttga ggtagaagac aatcacaatt ttgttgcaaa tgggctttta      120

gttcataac                                                              129


<210>  44
<211>  129
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Codon-optimized sequence #2 encoding Synechocystis sp. PCC6803 
       DnaX non-ligating split intein, C-terminal portion (SEQ ID NO:11)

<400>  44
atgagtccgc agtggcatac taatttcgag gaggtagagt cagtcactaa gggtcaggtg       60

gagaaggttt atgatttgga agtggaagac aatcataatt tcgtggcaaa tggtctgctt      120

gttcataac                                                              129


<210>  45
<211>  183
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Codon-optimized sequence #1 encoding Synechocystis sp. PCC6803 
       DnaX 6xHis-tagged non-ligating split intein, C-terminal portion 
       (SEQ ID NO:12)

<400>  45
atgcaccacc atcaccatca cgattacaaa gatgatgatg acaaaggtag cggatcatcg       60

ccacaatggc ataccaattt tgaggaggtg gagtcggtta caaaaggtca ggtggaaaag      120

gtatatgacc ttgaggtaga agacaatcac aattttgttg caaatgggct tttagttcat      180

aac                                                                    183


<210>  46
<211>  183
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Codon-optimized sequence #2 encoding Synechocystis sp. PCC6803 
       DnaX 6xHis-tagged non-ligating split intein, C-terminal portion 
       (SEQ ID NO:12)

<400>  46
atgcaccacc atcaccatca cgattacaaa gatgatgatg acaaaggaag tgggtccagt       60

ccgcagtggc atactaattt cgaggaggta gagtcagtca ctaagggtca ggtggagaag      120

gtttatgatt tggaagtgga agacaatcat aatttcgtgg caaatggtct gcttgttcat      180

aac                                                                    183


<210>  47
<211>  321
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Codon-optimized sequence encoding Synechocystis sp. PCC6803 DnaB 
       non-ligating split intein, N-terminal portion (SEQ ID NO:13)

<400>  47
atggctattt caggcgacag tctgatctct cttgcttcca cagggaagcg cgtatctatt       60

aaagatttgt tggacgaaaa agattttgaa atttgggcta tcaacgagca aaccatgaag      120

ctggaatctg caaaggtcag ccgcgtcttc tgcacaggta aaaaacttgt ttacattctt      180

aaaacgcgtc tgggtcgtac aatcaaggcg acagccaacc atcgcttttt aaccattgat      240

ggttggaagc gtctggatga gttgtctctg aaggagcaca tcgcgcttcc tcgtaaactg      300

gagtcgtcta gtttgcaatt g                                                321


<210>  48
<211>  147
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Codon-optimized sequence #1 encoding Synechocystis sp. PCC6803 
       DnaB non-ligating split intein, C-terminal portion (SEQ ID NO:14)

<400>  48
atgtcgccgg agatcgaaaa gttgtcccag tccgacatct actgggattc gatcgttagc       60

atcactgaga caggagtgga agaggtgttc gacttaacgg tgcctggacc ccataacttt      120

gtggcgaacg atatcatcgt tcacaac                                          147


<210>  49
<211>  147
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Codon-optimized sequence #2 encoding Synechocystis sp. PCC6803 
       DnaB non-ligating split intein, C-terminal portion (SEQ ID NO:14)

<400>  49
atgtctcccg agattgagaa actttcccag agcgacatct attgggatag tatcgtgagt       60

attactgaaa cgggggtgga ggaggtgttc gacttgaccg tccctggtcc tcataacttt      120

gttgcgaacg atattattgt tcacaac                                          147


<210>  50
<211>  201
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Codon-optimized sequence #1 encoding Synechocystis sp. PCC6803 
       DnaB 6xHis-tagged non-ligating split intein, C-terminal portion 
       (SEQ ID NO:15)

<400>  50
atgcaccacc atcaccatca cgattacaaa gatgatgatg acaaagggtc ggggtcttcg       60

ccggagatcg aaaagttgtc ccagtccgac atctactggg attcgatcgt tagcatcact      120

gagacaggag tggaagaggt gttcgactta acggtgcctg gaccccataa ctttgtggcg      180

aacgatatca tcgttcacaa c                                                201


<210>  51
<211>  201
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Codon-optimized sequence #2 encoding Synechocystis sp. PCC6803 
       DnaB 6xHis-tagged non-ligating split intein, C-terminal portion 
       (SEQ ID NO:15)

<400>  51
atgcaccacc atcaccatca cgattacaaa gatgatgatg acaaaggaag tggttcatct       60

cccgagattg agaaactttc ccagagcgac atctattggg atagtatcgt gagtattact      120

gaaacggggg tggaggaggt gttcgacttg accgtccctg gtcctcataa ctttgttgcg      180

aacgatatta ttgttcacaa c                                                201


<210>  52
<211>  267
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Codon-optimized sequence encoding Prochlorococcus cyanophage 
       P-SSM2 gp41-1 non-ligating split intein, N-terminal portion (SEQ 
       ID NO:16)

<400>  52
atggccctgg atctgaagac gcaagttcag acgccgcagg gtatgaagga aatctcgaac       60

attcaagtcg gtgatttagt gcttagcaat acgggctaca acgaagtcct gaacgtattt      120

cctaaatcaa aaaagaaatc ctacaagatt actttagaag acgggaagga gatcatttgt      180

agtgaagagc acttgtttcc tacccaaacc ggagagatga atatcagcgg tggtcttaag      240

gaaggtatgt gtttatacgt gaaagag                                          267


<210>  53
<211>  108
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Codon-optimized sequence #1 encoding Prochlorococcus cyanophage 
       P-SSM2 gp41-1 non-ligating split intein, C-terminal portion (SEQ 
       ID NO:17)

<400>  53
atgcttaaga aaatcttgaa gattgaggag ttagatgagc gcgaactgat tgacattgag       60

gtgtcaggaa accatctgtt ctatgccaac gacattctta cgcacaac                   108


<210>  54
<211>  108
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Codon-optimized sequence #2 encoding Prochlorococcus cyanophage 
       P-SSM2 gp41-1 non-ligating split intein, C-terminal portion (SEQ 
       ID NO:17)

<400>  54
atgttgaaaa agattttgaa gatcgaagag ttggacgaac gcgaattgat cgacatcgag       60

gtatcaggca accatttatt ttatgcgaat gacatcttaa cacacaac                   108


<210>  55
<211>  282
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Codon-optimized sequence encoding Clostridium thermocellum 
       CthBIL4 N115D non-ligating split intein 'C42', N-terminal portion
       (SEQ ID NO:18)

<400>  55
atggcttttg tagcgggcac catgattctt acagctaccg gcttagtcgc catcgaaaat       60

atcaaggcag gcgacaaggt aatcgcgaca aatcctgaga cttttgaggt cgccgagaag      120

actgtattgg aaacctatgt ccgtgagaca actgaattgc tgcacctgac gattggtggt      180

gaagtgatta aaaccacttt cgatcacccc ttctacgtaa aggatgtagg ttttgtggag      240

gccggtaaat tacaggttgg ggacaagctg ttagattcgc gc                         282


<210>  56
<211>  129
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Codon-optimized sequence #1 encoding Clostridium thermocellum 
       CthBIL4 N115D non-ligating split intein 'C42', C-terminal portion
       (SEQ ID NO:19)

<400>  56
atgggtaacg ttctggtggt cgaagaaaag aaacttgaga ttgcagacaa acccgtgaaa       60

gtctacgact tcaaggtgga cgactttcac acttaccacg tcggagataa tgaagtgctt      120

gtccataac                                                              129


<210>  57
<211>  129
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Codon-optimized sequence #2 encoding Clostridium thermocellum 
       CthBIL4 N115D non-ligating split intein 'C42', C-terminal portion
       (SEQ ID NO:19)

<400>  57
atggggaatg ttttagtcgt agaggagaaa aagttggaga ttgccgacaa gcctgttaaa       60

gtctatgact tcaaagtgga tgacttccac acctaccatg tgggagataa cgaagtgtta      120

gttcacaat                                                              129


<210>  58
<211>  360
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Codon-optimized sequence encoding Clostridium thermocellum 
       CthBIL4 N115D non-ligating split intein 'C16', N-terminal portion
       (SEQ ID NO:20)

<400>  58
atggcttttg tagcgggcac catgattctt acagctaccg gcttagtcgc catcgaaaat       60

atcaaggcag gcgacaaggt aatcgcgaca aatcctgaga cttttgaggt cgccgagaag      120

actgtattgg aaacctatgt ccgtgagaca actgaattgc tgcacctgac gattggtggt      180

gaagtgatta aaaccacttt cgatcacccc ttctacgtaa aggatgtagg ttttgtggag      240

gccggtaaat tacaggttgg ggacaagctg ttagattcgc gcggtaacgt tctggtggtc      300

gaagaaaaga aacttgagat tgcagacaaa cccgtgaaag tctacgactt caaggtggac      360


<210>  59
<211>  51
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Codon-optimized sequence #1 encoding Clostridium thermocellum 
       CthBIL4 N115D non-ligating split intein 'C16', C-terminal portion
       (SEQ ID NO:21)

<400>  59
atggactttc acacttacca cgtcggagat aatgaagtgc ttgtccataa c                51


<210>  60
<211>  51
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Codon-optimized sequence #2 encoding Clostridium thermocellum 
       CthBIL4 N115D non-ligating split intein 'C16', C-terminal portion
       (SEQ ID NO:21)

<400>  60
atggacttcc acacctacca tgtgggagat aacgaagtgt tagttcacaa t                51


<210>  61
<211>  507
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Codon-optimized sequence #2A encoding Synechocystis sp. PCC6803 
       DnaB 6xHis-tagged non-ligating mini-intein (SEQ ID NO:2)

<400>  61
atggcaatat cgggtgattc cctgatctca ttggcttcta ccggaaagcg cgtaagcatc       60

aaagacctgc ttgatgagaa agatttcgaa atctgggcga ttaacgagca aactatgaaa      120

ttggagtctg ctaaggtaag tcgcgtattc tgcactggga aaaagttagt ttatattttg      180

aaaacacgtt tagggcgcac tattaaggca acagcaaatc accgctttct gaccattgat      240

ggttggaaac gtctggatga gcttagtctt aaagaacaca tcgctttgcc tcgcaaactt      300

gaatcgtcat cgcttcaact ggcgtcaggc catcatcatc accatcatgg gggatcgggt      360

tcatctcccg agattgagaa actttcccag agcgacatct attgggatag tatcgtgagt      420

attactgaaa cgggggtgga ggaggtgttc gacttgaccg tccctggtcc tcataacttt      480

gttgcgaacg atattattgt tcacaac                                          507


<210>  62
<211>  507
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Codon-optimized sequence #2B encoding Synechocystis sp. PCC6803 
       DnaB 6xHis-tagged non-ligating mini-intein (SEQ ID NO:2)

<400>  62
atggcaatat cgggtgattc tctgatctcg ttggcttcta ccggaaagcg cgtaagcatc       60

aaagacctgc ttgatgagaa agatttcgaa atctgggcga ttaacgagca aactatgaaa      120

ttggagtctg ctaaggtaag tcgcgtattc tgcactggga aaaagttagt ttatattttg      180

aaaacacgtt tagggcgcac tattaaggca acagcaaatc accgctttct gaccattgat      240

ggttggaaac gtctggatga gcttagtctt aaagaacaca tcgctttgcc tcgcaaactt      300

gaatcgtcat cgcttcaact ggcgtcaggc catcatcatc accatcatgg gggatcgggt      360

tcatctcccg agattgagaa actttcccag agcgacatct attgggatag tatcgtgagt      420

attactgaaa cgggggtgga ggaggtgttc gacttgaccg tccctggtcc tcataacttt      480

gttgcgaacg atattattgt tcacaac                                          507


<210>  63
<211>  507
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Codon-optimized sequence #2C encoding Synechocystis sp. PCC6803 
       DnaB 6xHis-tagged non-ligating mini-intein (SEQ ID NO:2)

<400>  63
atggctatct caggtgattc gctgatttct ttggcttcta ccggaaagcg cgtaagcatc       60

aaagacctgc ttgatgagaa agatttcgaa atctgggcga ttaacgagca aactatgaaa      120

ttggagtctg ctaaggtaag tcgcgtattc tgcactggga aaaagttagt ttatattttg      180

aaaacacgtt tagggcgcac tattaaggca acagcaaatc accgctttct gaccattgat      240

ggttggaaac gtctggatga gcttagtctt aaagaacaca tcgctttgcc tcgcaaactt      300

gaatcgtcat cgcttcaact ggcgtcaggc catcatcatc accatcatgg gggatcgggt      360

tcatctcccg agattgagaa actttcccag agcgacatct attgggatag tatcgtgagt      420

attactgaaa cgggggtgga ggaggtgttc gacttgaccg tccctggtcc tcataacttt      480

gttgcgaacg atattattgt tcacaac                                          507


<210>  64
<211>  507
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Codon-optimized sequence #2D encoding Synechocystis sp. PCC6803 
       DnaB 6xHis-tagged non-ligating mini-intein (SEQ ID NO:2)

<400>  64
atggctattt caggcgattc actaatttca ttggcttcta ccggaaagcg cgtaagcatc       60

aaagacctgc ttgatgagaa agatttcgaa atctgggcga ttaacgagca aactatgaaa      120

ttggagtctg ctaaggtaag tcgcgtattc tgcactggga aaaagttagt ttatattttg      180

aaaacacgtt tagggcgcac tattaaggca acagcaaatc accgctttct gaccattgat      240

ggttggaaac gtctggatga gcttagtctt aaagaacaca tcgctttgcc tcgcaaactt      300

gaatcgtcat cgcttcaact ggcgtcaggc catcatcatc accatcatgg gggatcgggt      360

tcatctcccg agattgagaa actttcccag agcgacatct attgggatag tatcgtgagt      420

attactgaaa cgggggtgga ggaggtgttc gacttgaccg tccctggtcc tcataacttt      480

gttgcgaacg atattattgt tcacaac                                          507


<210>  65
<211>  507
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Codon-optimized sequence DnaB_Alt_v02 encoding Synechocystis sp. 
       PCC6803 DnaB 6xHis-tagged non-ligating mini-intein (SEQ ID NO:2)

<400>  65
atggctattt cgggcgattc actgataagt ttggcttcta ccggaaagcg cgtaagcatc       60

aaagacctgc ttgatgagaa agatttcgaa atctgggcga ttaacgagca aactatgaaa      120

ttggagtctg ctaaggtaag tcgcgtattc tgcactggga aaaagttagt ttatattttg      180

aaaacacgtt tagggcgcac tattaaggca acagcaaatc accgctttct gaccattgat      240

ggttggaaac gtctggatga gcttagtctt aaagaacaca tcgctttgcc tcgcaaactt      300

gaatcgtcat cgcttcaact ggcgtcaggc catcatcatc accatcatgg gggatcgggt      360

tcatctcccg agattgagaa actttcccag agcgacatct attgggatag tatcgtgagt      420

attactgaaa cgggggtgga ggaggtgttc gacttgaccg tccctggtcc tcataacttt      480

gttgcgaacg atattattgt tcacaac                                          507


<210>  66
<211>  507
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Codon-optimized sequence DnaB_Alt_v03 encoding Synechocystis sp. 
       PCC6803 DnaB 6xHis-tagged non-ligating mini-intein (SEQ ID NO:2)

<400>  66
atggccatat ctggtgactc gctgatcagt ttggcttcta ccggaaagcg cgtaagcatc       60

aaagacctgc ttgatgagaa agatttcgaa atctgggcga ttaacgagca aactatgaaa      120

ttggagtctg ctaaggtaag tcgcgtattc tgcactggga aaaagttagt ttatattttg      180

aaaacacgtt tagggcgcac tattaaggca acagcaaatc accgctttct gaccattgat      240

ggttggaaac gtctggatga gcttagtctt aaagaacaca tcgctttgcc tcgcaaactt      300

gaatcgtcat cgcttcaact ggcgtcaggc catcatcatc accatcatgg gggatcgggt      360

tcatctcccg agattgagaa actttcccag agcgacatct attgggatag tatcgtgagt      420

attactgaaa cgggggtgga ggaggtgttc gacttgaccg tccctggtcc tcataacttt      480

gttgcgaacg atattattgt tcacaac                                          507


<210>  67
<211>  507
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Codon-optimized sequence DnaB_KA encoding Synechocystis sp. 
       PCC6803 DnaB 4xHis-KA-tagged non-ligating mini-intein

<400>  67
atggccattt caggtgatag tttaatctcg ttagcctcaa cagggaagcg cgtgtctatc       60

aaagatctgc tggatgaaaa ggattttgaa atttgggcta tcaatgaaca aaccatgaag      120

ttggaatccg cgaaggtaag tcgtgtcttc tgtacgggaa aaaagctggt gtatatcctg      180

aagacccgtt tgggccgcac tatcaaggca accgctaatc atcgcttcct gaccattgac      240

ggatggaagc gtttagacga attgagcttg aaagaacata tcgctttacc gcgcaaattg      300

gaatccagta gccttcaact tgcctctgga catcatcatc ataaagccgg tggtagcggc      360

tcctccccag aaattgagaa gctttcacaa tctgacatct actgggacag tatcgtatct      420

attaccgaga cgggtgttga agaggtgttt gatcttacgg taccaggtcc gcataatttt      480

gtagctaatg acatcattgt gcacaac                                          507


<210>  68
<211>  507
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Codon-optimized sequence DnaB_KA_var2 encoding Synechocystis sp. 
       PCC6803 DnaB 4xHis-KA-tagged non-ligating mini-intein

<400>  68
atggccatct caggggactc gctgattagc ctggcatcca ccgggaagcg tgtttccatt       60

aaggacctgc tggacgagaa agattttgaa atctgggcaa tcaatgagca aacgatgaag      120

ttagagtcgg ctaaagtgtc ccgcgtcttt tgcaccggta aaaaattggt gtacattctt      180

aaaacccgtc ttggtcgtac cattaaagcg acagcgaacc atcgtttttt aacgatcgac      240

ggctggaagc gcctggacga gttgtcactg aaagaacaca ttgccttacc acgcaagtta      300

gaaagttcct cattacagct ggccagcggc catcaccacc acaaggccgg tgggtccgga      360

tcgtcacccg aaatcgaaaa attgtctcaa tcggatatct attgggatag cattgtctcc      420

atcacggaaa cgggcgtcga ggaagttttt gatcttacgg tgccgggacc acacaatttc      480

gtagccaatg acatcattgt gcataac                                          507


<210>  69
<211>  507
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Codon-optimized sequence DnaB_KA_var2-1 encoding Synechocystis 
       sp. PCC6803 DnaB 4xHis-KA-tagged non-ligating mini-intein

<400>  69
atggccattt caggtgattc gcttataagt ctggcatcca ccgggaagcg tgtttccatt       60

aaggacctgc tggacgagaa agattttgaa atctgggcaa tcaatgagca aacgatgaag      120

ttagagtcgg ctaaagtgtc ccgcgtcttt tgcaccggta aaaaattggt gtacattctt      180

aaaacccgtc ttggtcgtac cattaaagcg acagcgaacc atcgtttttt aacgatcgac      240

ggctggaagc gcctggacga gttgtcactg aaagaacaca ttgccttacc acgcaagtta      300

gaaagttcct cattacagct ggccagcggc catcaccacc acaaggccgg tgggtccgga      360

tcgtcacccg aaatcgaaaa attgtctcaa tcggatatct attgggatag cattgtctcc      420

atcacggaaa cgggcgtcga ggaagttttt gatcttacgg tgccgggacc acacaatttc      480

gtagccaatg acatcattgt gcataac                                          507


<210>  70
<211>  507
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Codon-optimized sequence DnaB_KA_var2-2 encoding Synechocystis 
       sp. PCC6803 DnaB 4xHis-KA-tagged non-ligating mini-intein

<400>  70
atggcgatat cgggtgattc tctaatcagt ctcgcatcca ccgggaagcg tgtttccatt       60

aaggacctgc tggacgagaa agattttgaa atctgggcaa tcaatgagca aacgatgaag      120

ttagagtcgg ctaaagtgtc ccgcgtcttt tgcaccggta aaaaattggt gtacattctt      180

aaaacccgtc ttggtcgtac cattaaagcg acagcgaacc atcgtttttt aacgatcgac      240

ggctggaagc gcctggacga gttgtcactg aaagaacaca ttgccttacc acgcaagtta      300

gaaagttcct cattacagct ggccagcggc catcaccacc acaaggccgg tgggtccgga      360

tcgtcacccg aaatcgaaaa attgtctcaa tcggatatct attgggatag cattgtctcc      420

atcacggaaa cgggcgtcga ggaagttttt gatcttacgg tgccgggacc acacaatttc      480

gtagccaatg acatcattgt gcataac                                          507


<210>  71
<211>  507
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Codon-optimized sequence DnaB_KA_var2-3_mutant encoding 
       Synechocystis sp. PCC6803 DnaB 4xHis-KA-tagged non-ligating 
       mini-intein

<400>  71
atggccatat ctggtgactc gctgatcagt cttgcatcca ccgggaagcg tgtttccatt       60

aaggacctgc tggacgagaa agattttgaa atctgggcaa tcaatgagca aacgatgaag      120

ttagagtcgg ctaaagtgtc ccgcgtcttt tgcaccggta aaaaattggt gtacattctt      180

aaaacccgtc ttggtcgtgc cattaaagcg acagcgaacc atcgtttttt aacgatcgac      240

ggctggaagc gcctggacga gttgtcactg aaagaacaca ttgccttacc acgcaagtta      300

gaaagttcct cattacagct ggccagcggc catcaccacc acaaggccgg tgggtccgga      360

tcgtcacccg aaatcgaaaa attgtctcaa tcggatatct attgggatag cattgtctcc      420

atcacggaaa cgggcgtcga ggaagttttt gatcttacgg tgccgggacc acacaatttc      480

gtagccaatg acatcattgt gcataac                                          507


<210>  72
<211>  507
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Codon-optimized sequence DnaB_KA_var2-4 encoding Synechocystis 
       sp. PCC6803 DnaB 4xHis-KA-tagged non-ligating mini-intein

<400>  72
atggctatat ctggagattc acttatcagt ctcgcatcca ccgggaagcg tgtttccatt       60

aaggacctgc tggacgagaa agattttgaa atctgggcaa tcaatgagca aacgatgaag      120

ttagagtcgg ctaaagtgtc ccgcgtcttt tgcaccggta aaaaattggt gtacattctt      180

aaaacccgtc ttggtcgtac cattaaagcg acagcgaacc atcgtttttt aacgatcgac      240

ggctggaagc gcctggacga gttgtcactg aaagaacaca ttgccttacc acgcaagtta      300

gaaagttcct cattacagct ggccagcggc catcaccacc acaaggccgg tgggtccgga      360

tcgtcacccg aaatcgaaaa attgtctcaa tcggatatct attgggatag cattgtctcc      420

atcacggaaa cgggcgtcga ggaagttttt gatcttacgg tgccgggacc acacaatttc      480

gtagccaatg acatcattgt gcataac                                          507


<210>  73
<211>  507
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Codon-optimized sequence DnaB_KA_var2-5 encoding Synechocystis 
       sp. PCC6803 DnaB 4xHis-KA-tagged non-ligating mini-intein

<400>  73
atggccattt ctggcgattc gctaattagt cttgcatcca ccgggaagcg tgtttccatt       60

aaggacctgc tggacgagaa agattttgaa atctgggcaa tcaatgagca aacgatgaag      120

ttagagtcgg ctaaagtgtc ccgcgtcttt tgcaccggta aaaaattggt gtacattctt      180

aaaacccgtc ttggtcgtac cattaaagcg acagcgaacc atcgtttttt aacgatcgac      240

ggctggaagc gcctggacga gttgtcactg aaagaacaca ttgccttacc acgcaagtta      300

gaaagttcct cattacagct ggccagcggc catcaccacc acaaggccgg tgggtccgga      360

tcgtcacccg aaatcgaaaa attgtctcaa tcggatatct attgggatag cattgtctcc      420

atcacggaaa cgggcgtcga ggaagttttt gatcttacgg tgccgggacc acacaatttc      480

gtagccaatg acatcattgt gcataac                                          507


<210>  74
<211>  507
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Codon-optimized sequence DnaB_KA_var2-6 encoding Synechocystis 
       sp. PCC6803 DnaB 4xHis-KA-tagged non-ligating mini-intein

<400>  74
atggctattt cgggcgattc actgataagt ctagcatcca ccgggaagcg tgtttccatt       60

aaggacctgc tggacgagaa agattttgaa atctgggcaa tcaatgagca aacgatgaag      120

ttagagtcgg ctaaagtgtc ccgcgtcttt tgcaccggta aaaaattggt gtacattctt      180

aaaacccgtc ttggtcgtac cattaaagcg acagcgaacc atcgtttttt aacgatcgac      240

ggctggaagc gcctggacga gttgtcactg aaagaacaca ttgccttacc acgcaagtta      300

gaaagttcct cattacagct ggccagcggc catcaccacc acaaggccgg tgggtccgga      360

tcgtcacccg aaatcgaaaa attgtctcaa tcggatatct attgggatag cattgtctcc      420

atcacggaaa cgggcgtcga ggaagttttt gatcttacgg tgccgggacc acacaatttc      480

gtagccaatg acatcattgt gcataac                                          507


<210>  75
<211>  507
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Codon-optimized sequence DnaB_KH encoding Synechocystis sp. 
       PCC6803 DnaB HHHHKH-tagged non-ligating mini-intein

<400>  75
atggctatta gcggtgactc cttaatctct ttagctagta caggtaagcg cgtatcaatt       60

aaggacttac tggatgaaaa ggatttcgag atctgggcaa ttaacgagca gactatgaaa      120

ttagaatctg ccaaggtatc gcgtgttttt tgcactggta aaaagttggt ttatattctt      180

aaaactcgtc tgggccgtac gattaaagcc actgccaatc accgttttct taccattgat      240

ggctggaagc gtttggatga actgtcactt aaagaacata ttgcgcttcc tcgtaaactt      300

gaatcctcgt ctttgcaact tgcatcaggt caccatcacc acaagcatgg aggctcaggg      360

agttcgcccg aaattgaaaa gctgtctcag tctgatatct actgggattc tattgtatcc      420

attactgaaa ccggtgtgga ggaagtcttc gatttaacgg ttcctggtcc gcacaatttt      480

gtagctaatg atatcattgt ccacaac                                          507


<210>  76
<211>  507
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Codon-optimized sequence DnaB_KS encoding Synechocystis sp. 
       PCC6803 DnaB HHHHKS-tagged non-ligating mini-intein

<400>  76
atggcaatct ctggtgacag tttgatctct ttagcatcaa ctggcaagcg tgtatccatt       60

aaggatttgc tggatgaaaa ggattttgaa atctgggcga ttaatgaaca aacgatgaaa      120

ttagagagcg cgaaagtcag ccgcgtgttt tgtaccggga agaaattggt atacatcctg      180

aagacgcgcc ttggccgcac tattaaggcg acagcgaatc accgtttctt aacgatcgac      240

ggctggaagc gcctggatga actgagctta aaagagcaca tcgccctgcc acgcaagctg      300

gaatcttcta gcttacaact ggcgtcgggt catcaccacc ataagtcggg tggttctggg      360

tcttcgccgg aaatcgaaaa actgtcacag tccgatattt actgggactc tatcgtcagt      420

attaccgaga ctggcgtcga ggaggtattt gacttaacgg tgcctgggcc tcataacttt      480

gtggctaatg acattattgt gcataat                                          507


<210>  77
<211>  507
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Codon-optimized sequence DnaB_KT encoding Synechocystis sp. 
       PCC6803 DnaB HHHHKT-tagged non-ligating mini-intein

<400>  77
atggcgattt cgggggactc gcttatttca ctggcttcca ccgggaagcg cgtatctatc       60

aaggatttat tggatgaaaa agacttcgag atttgggcga ttaatgagca gaccatgaag      120

cttgaaagtg cgaaagtctc ccgcgtgttc tgcaccggaa agaagttggt ctacattctt      180

aaaacgcgcc tgggtcgtac aatcaaagcg acggctaacc atcgtttctt gaccattgat      240

ggttggaagc gtttggacga actgagtctg aaagagcaca tcgcattacc ccgcaagctt      300

gagtccagtt cgttgcagct tgccagcgga caccatcatc acaaaactgg aggatcaggt      360

agtagtcctg aaatcgagaa gttgtcacag tccgatattt attgggattc tattgtgagt      420

attactgaaa caggcgtaga agaagttttc gacttaacag tccctggtcc acataacttc      480

gtggccaatg acatcatcgt gcataac                                          507


<210>  78
<211>  465
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Codon-optimized sequence DnaB_M86 encoding Synechocystis sp. 
       PCC6803 DnaB non-ligating mini-intein

<400>  78
atggcaatta gcggagattc tcttatttcg ttagcatcga ccgggaagcg cgtcccaatc       60

aaagaccttt taggagagaa agatttcgag atttgggcca tcaatgagca gacgatgaag      120

ttggaaagcg caaaagtgtc tcgtgtgttt tgtacgggaa agaaattagt atacacgttg      180

aaaacgcgct tagggcgtac catcaaagcg actgctaacc accgcttttt gaccattgac      240

ggctggaaac gtctggatga gttatcattg aaggagcata tcgctttacc gcgtaagtta      300

gagtcgtcat ccttacaact ggctcccgaa atcgagaaac ttccacagtc cgacatttac      360

tgggacccta tcgtctccat caccgagaca ggggtggaag aggtcttcga tttaaccgtc      420

ccggggttac gcaacttcgt tgcgaacgac attatcgttc ataac                      465


<210>  79
<211>  507
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Codon-optimized sequence DnaB_v03 encoding Synechocystis sp. 
       PCC6803 DnaB 6xHis-tagged non-ligating mini-intein (SEQ ID NO:2)

<400>  79
atggcgatta gcggcgatag tctgattagt ctggccagca ccggcaagcg tgtgagcatc       60

aaggatctgc tggacgaaaa ggacttcgag atctgggcca tcaacgagca gaccatgaaa      120

ctggagagcg ccaaagttag ccgcgttttc tgcaccggta agaagctcgt gtacatcctc      180

aagacccgtc tgggtcgtac catcaaagcg acggcgaacc atcgctttct gaccatcgat      240

ggctggaaac gtctggacga actgagtctg aaagagcata tcgcgctgcc acgcaagctg      300

gaaagcagta gtctgcagct ggcgagcggc caccatcatc accatcatgg cggcagcggt      360

agcagcccgg aaatcgagaa gctgagccag agcgacatct actgggatag catcgtgagc      420

atcaccgaga cgggcgtgga agaagtgttt gatctgacgg ttccgggccc gcacaacttc      480

gtggccaacg acatcatcgt gcacaac                                          507


<210>  80
<211>  507
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Codon-optimized sequence DnaB_v04 encoding Synechocystis sp. 
       PCC6803 DnaB 6xHis-tagged non-ligating mini-intein (SEQ ID NO:2)

<400>  80
atggcgatca gcggcgatag tctgatcagt ctggcgagta ccggcaaacg cgtgagcatc       60

aaggatctgc tcgatgagaa ggacttcgag atctgggcga tcaacgagca gaccatgaag      120

ctggagagcg cgaaagtgag tcgcgttttc tgcacgggca agaagctggt gtacatcctc      180

aaaacccgtc tcggccgcac cattaaggcg accgccaatc accgctttct gaccattgat      240

ggctggaaac gtctggatga actgagtctg aaggaacaca tcgcgctgcc acgcaaactg      300

gagagtagca gcctccagct ggccagcggt catcatcacc accatcatgg tggtagcggt      360

agcagcccgg aaattgagaa gctcagccag agcgacatct actgggatag catcgtgagc      420

atcaccgaaa cgggtgtgga agaagttttc gatctgacgg ttccgggccc gcacaacttt      480

gtggcgaacg atatcatcgt gcacaac                                          507


<210>  81
<211>  507
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Codon-optimized sequence DnaB_v05 encoding Synechocystis sp. 
       PCC6803 DnaB 6xHis-tagged non-ligating mini-intein (SEQ ID NO:2)

<400>  81
atggctattt caggagatag tctaatatct ttggctagca ccggcaagcg cgtatccatc       60

aaagaccttc tggacgagaa ggacttcgag atctgggcaa ttaacgaaca aaccatgaaa      120

ttggagagcg cgaaagttag tcgtgtgttt tgtaccggta agaagttggt gtacatcctg      180

aaaacccgtc tgggccgtac cattaaagct accgcgaacc accgctttct gaccattgat      240

ggttggaaac gtttggatga gctgagtctg aaagagcaca ttgcgttacc gcgtaaactt      300

gagagcagct ccctgcaatt ggctagcggt catcaccacc accaccatgg tggttccggc      360

tcatccccgg agattgaaaa actgagccag agcgatattt actgggatag catcgtgtct      420

atcaccgaaa ccggtgtcga ggaagttttc gacctgacgg tgccgggtcc gcataacttt      480

gttgcgaatg acatcatcgt tcacaac                                          507


<210>  82
<211>  507
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Codon-optimized sequence DnaB_v06 encoding Synechocystis sp. 
       PCC6803 DnaB 6xHis-tagged non-ligating mini-intein (SEQ ID NO:2)

<400>  82
atggctattt caggagatag tctaatatct ctcgcgagca ccggcaagcg cgtgtccatc       60

aaagacttgc ttgacgaaaa ggactttgag atctgggcaa ttaacgaaca aaccatgaaa      120

cttgagagcg ctaaagtgtc acgtgtgttc tgcaccggta agaaattggt gtacatcctg      180

aaaacgcgtc tgggtcgtac catcaaggcg acggcgaatc atcgctttct gaccatcgat      240

ggttggaaga gactggatga gctgagctta aaggagcaca ttgcactgcc gcgtaaattg      300

gaaagctctt ccctgcaatt ggcgagcggc caccaccatc accaccacgg cggatctggc      360

tcgtccccgg agatcgagaa gttaagccag tcggacatct actgggatag catcgtgtct      420

atcaccgaga cgggtgtcga ggaagttttt gatttgacgg ttccgggtcc gcataacttt      480

gtggcgaatg atattatcgt gcataat                                          507


<210>  83
<211>  507
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Codon-optimized sequence DnaB_v07 encoding Synechocystis sp. 
       PCC6803 DnaB 6xHis-tagged non-ligating mini-intein (SEQ ID NO:2)

<400>  83
atggcaatct ctggtgactc tctgatttct ctggctagca cgggtaaacg cgtttcgatc       60

aaagatcttc tggatgagaa agatttcgag atctgggcga ttaacgaaca gaccatgaaa      120

ttagagagcg ccaaggttag ccgcgtcttt tgcacgggta agaaactggt gtatatcttg      180

aaaacgcgtc tgggccgcac tattaaggcc accgccaacc atcgcttcct gaccattgac      240

ggttggaagc gcttggatga actgagcctg aaagaacaca tcgcgctgcc gcgtaaactg      300

gagtccagct ccttgcaact ggcgagcggt catcatcacc accatcatgg tggcagcggt      360

agcagcccag agatcgaaaa gttgagccag agcgacattt actgggactc cattgttagc      420

attaccgaaa cgggcgtcga ggaagttttt gatctgaccg tgccaggtcc gcacaatttt      480

gtcgcaaacg atatcatcgt tcacaat                                          507


<210>  84
<211>  507
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Codon-optimized sequence DnaB_v08 encoding Synechocystis sp. 
       PCC6803 DnaB 6xHis-tagged non-ligating mini-intein (SEQ ID NO:2)

<400>  84
atggcgatct ctggcgactc tctgatttct ctggctagca ctggtaagcg tgtttcgatt       60

aaagacttgc tggatgagaa agattttgag atctgggcaa tcaacgaaca gactatgaaa      120

ttggagtcgg ccaaggtcag ccgcgtgttt tgcaccggta agaaactggt ttacatcctg      180

aaaacgcgtc tgggtcgtac gattaaggca acggcgaatc accgcttcct gaccatcgac      240

ggctggaagc gcttagacga actgagcctg aaagaacata ttgcgctgcc gcgtaaactg      300

gagagcagca gcctgcagct ggcgagcggt caccaccacc atcaccatgg cggctctggc      360

agcagcccgg aaattgaaaa gctgagccaa tccgatatct actgggatag catcgtgagc      420

attaccgaaa ccggcgtcga ggaagttttc gacctgaccg ttccgggtcc gcataacttt      480

gttgcgaatg acattatcgt gcataat                                          507


<210>  85
<211>  507
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Codon-optimized sequence DnaB_v09 encoding Synechocystis sp. 
       PCC6803 DnaB 6xHis-tagged non-ligating mini-intein (SEQ ID NO:2)

<400>  85
atggccatca gcggcgatag cctcatcagt ctggcgagca ccggtaagcg tgtgagcatc       60

aaggatctgc tggacgagaa ggacttcgag atttgggcca tcaacgagca gaccatgaaa      120

ctcgagagcg ccaaggtgag ccgcgttttc tgcacgggca agaagctcgt gtacattctg      180

aaaacccgtc tgggtcgcac gatcaaagcg acggcgaatc atcgctttct gaccatcgat      240

ggctggaaac gtctggatga actcagtctg aaggagcaca tcgcgctgcc gcgcaaactg      300

gaaagcagca gtctgcagct ggcgagcggt catcatcatc accatcatgg cggcagcggc      360

agcagtccgg aaatcgagaa actgagccag agcgacatct actgggacag catcgtgagc      420

atcaccgaaa ccggcgtgga agaagtgttt gatctgaccg tgccgggtcc gcacaacttc      480

gttgcgaacg acatcatcgt gcacaac                                          507


<210>  86
<211>  507
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Codon-optimized sequence DnaB_v10 encoding Synechocystis sp. 
       PCC6803 DnaB 6xHis-tagged non-ligating mini-intein (SEQ ID NO:2)

<400>  86
atggctattt caggagatag tctaatatct ttggctagca ccggcaaacg tgtttcgatc       60

aaggacctcc tggacgagaa ggacttcgag atctgggcaa ttaatgaaca aaccatgaaa      120

ctagagtctg cgaaagtttc tcgtgttttc tgcaccggca agaagctggt ttacatcctg      180

aaaacccgtc tgggtcgtac cattaaggct accgccaacc accgcttcct gacaatcgac      240

ggctggaagc gcctggatga actgtccctg aaagaacata ttgcactgcc gcgtaaacta      300

gagtctagct cattacaact ggcgtcaggt catcaccacc accaccacgg cggtagcggc      360

agtagcccgg aaattgaaaa gctgtctcag agcgatatct actgggattc catcgtgagc      420

atcaccgaaa ccggtgtgga ggaagttttt gatcttacgg tgccgggtcc gcataatttt      480

gtggcgaacg acattattgt gcataac                                          507


<210>  87
<211>  507
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Codon-optimized sequence DnaB_v11 encoding Synechocystis sp. 
       PCC6803 DnaB 6xHis-tagged non-ligating mini-intein (SEQ ID NO:2)

<400>  87
atggcaattt ctggtgactc cctgatctct ctggcttcta cgggcaagcg tgtttcgatt       60

aaagatttac tggatgagaa agacttcgag atttgggcga ttaacgaaca gaccatgaaa      120

ctggagagcg cgaaagttag ccgtgtgttt tgtaccggta agaaactggt ctatattctg      180

aaaacccgtc tgggccgtac cattaaagcg accgcgaatc atcgcttcct gaccatcgat      240

ggctggaagc gtttggacga actgagcctg aaagaacaca ttgcactgcc gcgcaagctt      300

gagtcgtcct ccctgcaact ggccagcggc caccaccatc atcaccacgg tggcagcggc      360

agcagcccgg aaattgaaaa gctgtcgcaa agcgacatct actgggattc catcgttagc      420

atcacggaaa cgggtgtaga ggaagtgttt gatctgactg tgccgggtcc gcacaatttc      480

gtcgcgaacg atatcatcgt gcataat                                          507


<210>  88
<211>  507
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Codon-optimized sequence DnaB_v12 encoding Synechocystis sp. 
       PCC6803 DnaB 6xHis-tagged non-ligating mini-intein (SEQ ID NO:2)

<400>  88
atggccatca gcggtgatag tctcattagt ctggcgagta ccggtaagcg cgtgagtatt       60

aaggatctgc tggacgagaa ggactttgag atttgggcga tcaacgagca gacgatgaaa      120

ctggagagcg cgaaggttag tcgtgtgttc tgcaccggta agaagctggt gtacatcctc      180

aagacccgcc tcggccgcac gattaaagcc acggcgaatc accgttttct gaccatcgat      240

ggctggaaac gtctggatga gctgagtctg aaagaacata tcgcgctgcc acgcaagctg      300

gaaagcagca gtctgcagct ggccagcggc catcatcacc atcatcatgg tggcagcggc      360

agcagcccag agatcgagaa gctgagccag agcgacatct actgggatag catcgttagc      420

attaccgaga cgggcgttga agaagtgttc gatctcaccg tgccgggccc gcataatttc      480

gtggccaatg acatcatcgt gcacaat                                          507


<210>  89
<211>  507
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Codon-optimized sequence DnaB_v13 encoding Synechocystis sp. 
       PCC6803 DnaB 6xHis-tagged non-ligating mini-intein (SEQ ID NO:2)

<400>  89
atggctattt caggagatag tctaatatct ttggcgtcta cgggcaaacg cgtgtctatc       60

aaggatttgc tggacgagaa agacttcgaa atctgggcga ttaacgagca aacgatgaaa      120

ctggaaagcg cgaaagtttc tcgcgtgttt tgtaccggca agaaactggt gtatatcctg      180

aaaacccgtc tgggtcgcac catcaaggcc accgcgaacc accgtttcct gacgatcgac      240

ggctggaaac gcctggacga gctcagcctt aaggagcaca ttgcgttacc gcgtaaattg      300

gagagcagct ctctgcaact ggcgagcggt catcaccacc accaccatgg tggctctggc      360

tccagcccgg agatcgaaaa attgtctcag agcgatattt attgggatag catcgttagc      420

atcaccgaaa ccggtgttga agaagtcttt gatctgaccg ttccgggtcc gcataacttc      480

gtggcgaatg acatcattgt tcacaac                                          507


<210>  90
<211>  507
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Codon-optimized sequence DnaB_v14 encoding Synechocystis sp. 
       PCC6803 DnaB 6xHis-tagged non-ligating mini-intein (SEQ ID NO:2)

<400>  90
atggcaattt ccggtgactc tctgatctct ctggcatcta ctggcaaacg cgtatcgatc       60

aaagatttgc tggacgagaa agattttgag atctgggcga ttaacgaaca gaccatgaaa      120

ctggagagcg caaaagttag ccgcgtgttc tgcaccggca agaaactggt ttacattctg      180

aaaacgcgtc tgggtcgcac catcaaggca accgctaatc accgtttcct gactatcgac      240

ggctggaaga gactggatga actgagcttg aaagaacaca tcgcgctgcc gcgtaaattg      300

gagagcagca gcctgcaact ggcatccggt catcaccatc accaccacgg tggcagcggc      360

agcagcccgg aaatcgaaaa gctgtcacag tctgatatct actgggatag catcgtcagc      420

attaccgaga ctggtgtgga agaggttttc gacctgacgg tcccgggtcc gcacaacttt      480

gtagcgaacg acattattgt tcacaat                                          507


<210>  91
<211>  507
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Codon-optimized sequence DnaB_v15 encoding Synechocystis sp. 
       PCC6803 DnaB 6xHis-tagged non-ligating mini-intein (SEQ ID NO:2)

<400>  91
atggcgatct ctggcgactc tctgatttct ctggcatcta ctggcaagcg tgtctcgatc       60

aaagacttgt tggacgagaa agatttcgag atttgggcga ttaatgaaca gaccatgaaa      120

ctggagagcg caaaggttag ccgtgtcttt tgcaccggta agaaactggt ctatatcctg      180

aaaacccgcc tgggtcgtac catcaaggcc actgccaacc accgtttcct gaccattgac      240

ggttggaaac gtctggatga actgagcctg aaagaacaca ttgcgctgcc gcgcaaatta      300

gagagcagca gcctgcagtt ggcgtcgggt catcatcatc accaccacgg tggcagcggt      360

agctccccgg aaatcgagaa actgagccaa agcgatatct attgggacag catcgttagc      420

attaccgaaa cgggtgttga ggaagtgttc gatttgaccg tcccgggtcc gcataacttt      480

gtggcgaatg acatcattgt ccacaac                                          507


<210>  92
<211>  507
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Codon-optimized sequence DnaB_v16 encoding Synechocystis sp. 
       PCC6803 DnaB 6xHis-tagged non-ligating mini-intein (SEQ ID NO:2)

<400>  92
atggcaatct ctggtgactc cctgatttct ctggcatcta ctggcaagcg cgttagcatc       60

aaagacctgt tggacgagaa agatttcgag atctgggcga tcaatgaaca aaccatgaaa      120

ttggagagcg ctaaggtcag ccgtgttttc tgtaccggta agaaactggt gtacatcctg      180

aaaacgcgtc tgggccgtac gattaaggca acggcgaacc atcgttttct gacgatcgat      240

ggctggaagc gtctggatga actgagcctt aaagaacata ttgccctgcc gcgcaaactg      300

gagagcagca gcctgcaact ggcgagcggt caccaccatc accatcacgg tggctcaggt      360

agcagccctg agattgaaaa gctgagccag agcgacatct actgggatag cattgtgagc      420

attaccgaaa ccggtgtgga agaggttttc gatttgactg tgccgggtcc acacaacttc      480

gtcgcaaacg acatcatcgt ccataat                                          507


<210>  93
<211>  507
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Codon-optimized sequence DnaB_v17 encoding Synechocystis sp. 
       PCC6803 DnaB 6xHis-tagged non-ligating mini-intein (SEQ ID NO:2)

<400>  93
atggcaattt ctggtgattc tctgatcagc ctggcgtcga ctggtaagcg tgtttctatc       60

aaagatctgc tggatgagaa agattttgag atctgggcca tcaatgaaca aaccatgaaa      120

ctggagagcg caaaggtcag ccgtgtgttt tgtaccggca agaaactggt ttacattctg      180

aaaacgcgcc tgggccgtac cattaaagcg acggcaaacc accgcttcct gaccatcgac      240

ggttggaagc gcctggacga attgagcctg aaagaacaca tcgcgttgcc acgtaaactg      300

gagtcgtcaa gcttacagtt ggcgtccggc caccatcatc accaccacgg tggcagcggt      360

agcagccctg agattgaaaa gctgagccaa agcgacattt actgggattc catcgtgagc      420

attacggaaa ccggtgtcga ggaagtgttc gacctgacgg tcccgggtcc gcacaatttt      480

gtggctaacg acatcattgt acataat                                          507


<210>  94
<211>  507
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Codon-optimized sequence DnaB_v18 encoding Synechocystis sp. 
       PCC6803 DnaB 6xHis-tagged non-ligating mini-intein (SEQ ID NO:2)

<400>  94
atggctattt ctggtgactc tctgatttct ctggcatcca ccggcaaacg tgtttcgatc       60

aaagatttgc tggatgagaa agattttgag atttgggcga tcaacgaaca gaccatgaaa      120

ctggagagcg cgaaagtttc ccgtgtgttc tgcaccggta agaaactggt gtatatctta      180

aagacccgtc tgggtcgcac cattaaggca accgcgaatc atcgcttctt gactatcgac      240

ggttggaaac gtctggatga actgtcgctg aaagaacaca ttgctctgcc gcgcaaactg      300

gagtcctcta gcctgcagct ggcgagcggt catcatcacc accaccacgg cggcagcggt      360

agctccccag agatcgagaa actgtcccag agcgacatct attgggacag catcgtcagc      420

atcactgaaa cgggtgttga agaggttttc gacctgacgg tgccgggtcc gcataatttt      480

gtggcgaatg acattatcgt tcacaac                                          507


<210>  95
<211>  507
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Codon-optimized sequence DnaB_v19 encoding Synechocystis sp. 
       PCC6803 DnaB 6xHis-tagged non-ligating mini-intein (SEQ ID NO:2)

<400>  95
atggccatca gcggcgatag cctcatcagt ctggccagta cgggtaagcg tgtgagcatt       60

aaggatctgc tggatgagaa ggacttcgag atctgggcga tcaacgaaca gacgatgaag      120

ctggagagtg ccaaggtgag ccgcgtgttc tgtacgggca agaagctggt gtacattctg      180

aaaacccgcc tcggccgtac catcaaagcc accgcgaatc accgtttcct caccatcgat      240

ggttggaaac gtctggacga actgagtctg aaagaacaca ttgcgctgcc acgcaagctg      300

gaaagcagca gtctccagct ggcgagtggt caccaccatc accaccatgg tggtagcggc      360

agtagcccag agattgagaa gctgagccag agcgatatct actgggatag catcgtgagc      420

atcacggaaa cgggcgtgga agaggttttt gatctgaccg tgccgggccc gcacaatttc      480

gtggcgaacg acatcatcgt gcacaac                                          507


<210>  96
<211>  507
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Codon-optimized sequence DnaB_v20 encoding Synechocystis sp. 
       PCC6803 DnaB 6xHis-tagged non-ligating mini-intein (SEQ ID NO:2)

<400>  96
atggcgatta gcggcgatag tctgatcagt ctggccagca ccggtaaacg cgtgagcatc       60

aaagatctgc tggacgaaaa ggactttgag atctgggcca tcaacgagca gacgatgaag      120

ctggaaagcg cgaaagtgag tcgcgtgttc tgtacgggca agaagctcgt gtacattctg      180

aaaacccgtc tgggtcgcac cattaaagcg accgcgaatc accgctttct gaccattgat      240

ggttggaaac gtctggatga gctgagtctg aaagaacaca tcgcgctgcc acgcaaactg      300

gaaagcagta gtctgcagct ggcgagcggc caccatcacc accaccatgg tggcagcggc      360

agcagcccgg aaatcgagaa gctcagccag agcgacatct actgggacag catcgtgagt      420

atcaccgaga cgggcgtgga ggaagtgttt gatctgaccg ttccgggccc gcacaacttc      480

gtggccaacg acatcatcgt gcacaac                                          507


<210>  97
<211>  507
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Codon-optimized sequence DnaB_v21 encoding Synechocystis sp. 
       PCC6803 DnaB 6xHis-tagged non-ligating mini-intein (SEQ ID NO:2)

<400>  97
atggccatca gcggcgatag tctgatcagt ctggcgagca cgggtaaacg cgttagcatc       60

aaagatctgc tggacgagaa ggacttcgaa atctgggcga tcaacgagca gacgatgaaa      120

ctggagagcg ccaaggttag ccgtgtgttt tgcacgggca agaagctggt gtacatcctc      180

aaaacgcgcc tcggtcgtac cattaaagcc accgcgaacc accgctttct caccatcgat      240

ggctggaaac gtctggatga gctcagtctg aaagaacaca tcgccctccc acgtaaactg      300

gaaagcagta gtctgcagct ggccagtggc caccatcatc accatcacgg cggtagcggc      360

agtagcccag agatcgagaa gctgagccag agcgacatct actgggacag catcgtgagc      420

atcacggaaa ccggtgttga agaagttttc gatctgacgg tgccgggccc gcataacttc      480

gtggccaacg atatcatcgt gcacaac                                          507


<210>  98
<211>  507
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Codon-optimized sequence DnaB_v22 encoding Synechocystis sp. 
       PCC6803 DnaB 6xHis-tagged non-ligating mini-intein (SEQ ID NO:2)

<400>  98
atggcgatca gtggcgatag tctgatcagt ctcgcgagca ccggcaaacg cgtgagcatc       60

aaggatctgc tggacgagaa agacttcgaa atttgggcca tcaacgagca gaccatgaaa      120

ctggagagcg ccaaggttag ccgcgtgttc tgcaccggta agaagctggt gtacatcctc      180

aaaacccgtc tgggccgcac gatcaaagcg accgcgaacc atcgcttcct cacgatcgat      240

ggctggaaac gcctcgacga actcagtctg aaagaacaca ttgcgctgcc gcgtaaactc      300

gaaagtagca gtctgcagct ggcgagtggc catcatcacc atcatcacgg cggtagcggc      360

agcagtccag aaatcgagaa actgagccag agcgacatct actgggacag catcgtgagc      420

atcaccgaaa cgggcgttga ggaagtgttt gatctcaccg tgccgggtcc acacaacttt      480

gtggcgaacg atatcattgt gcacaac                                          507


<210>  99
<211>  507
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Codon-optimized sequence DnaB_v23 encoding Synechocystis sp. 
       PCC6803 DnaB 6xHis-tagged non-ligating mini-intein (SEQ ID NO:2)

<400>  99
atggctattt caggagatag tctaatatct ctggcatcca ccggtaagcg cgtttccatc       60

aaagacttgc tggatgagaa agatttcgaa atttgggcaa ttaacgaaca gaccatgaaa      120

ttggagagcg ctaaggttag ccgcgtgttt tgtaccggca agaagttagt ttacatcctg      180

aaaacccgtc tgggccgtac cattaaggcg accgcgaacc atcgttttct taccatcgat      240

ggttggaaac gtctcgacga gctgagcctg aaagaacata ttgctctgcc ccgtaaattg      300

gagtcgagca gcttgcaact ggcctccggt catcaccacc accaccacgg tggcagcggc      360

tcttcgccgg agatcgaaaa actgtcccag tccgacattt actgggacag cattgtatca      420

atcaccgaaa ccggtgttga ggaagttttc gatttgacgg tcccgggtcc gcataacttt      480

gttgcgaatg atatcatcgt gcacaat                                          507


<210>  100
<211>  507
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Codon-optimized sequence DnaB_v24 encoding Synechocystis sp. 
       PCC6803 DnaB 6xHis-tagged non-ligating mini-intein (SEQ ID NO:2)

<400>  100
atggctatat ctggagatag tctaatttca ttggcgagca caggcaagcg cgtgagcatc       60

aaggaccttt tggacgagaa ggacttcgaa atctgggcaa ttaacgaaca aaccatgaaa      120

ttggagtctg ctaaagtgag ccgtgtgttc tgcaccggta agaaattggt ttacattctg      180

aaaacccgtc tgggtcgtac gattaaggcg accgcgaacc atcgttttct gaccatcgat      240

ggctggaagc gcctggacga gctctctctg aaagaacaca tcgcactgcc gcgtaaatta      300

gagagcagca gcctccaact ggcgagcggc caccatcacc accaccatgg tggcagcggc      360

agcagcccgg aaattgaaaa actgtctcaa tccgatatct actgggactc gattgttagc      420

atcaccgaaa ccggtgtcga agaggttttc gacctgacgg tgccgggtcc gcacaacttc      480

gtggccaacg acatcatcgt gcataat                                          507


<210>  101
<211>  507
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Codon-optimized sequence DnaB_v25 encoding Synechocystis sp. 
       PCC6803 DnaB 6xHis-tagged non-ligating mini-intein (SEQ ID NO:2)

<400>  101
atggctatat ctggagatag tctaatttca ttggcgtcaa cgggcaaacg cgtgtcgatc       60

aaggacctcc tggacgagaa ggacttcgag atctgggcaa ttaatgaaca gaccatgaaa      120

cttgagtctg cgaaggtgag ccgcgtgttc tgcaccggca agaaactagt gtacatcctg      180

aaaacccgtc tgggtcgtac cattaaagcg actgccaacc accgtttctt aaccattgat      240

ggttggaagc gcctggacga actgagcctg aaagagcaca ttgctctgcc gcgtaaattg      300

gaaagcagca gcctgcaatt ggcttctggt catcaccacc accaccacgg cggcagcggt      360

tcgtccccgg aaatcgaaaa actgagccaa tccgacatct actgggattc tatcgtgtcc      420

attaccgaaa ccggtgtgga ggaggtgttc gacctgacgg ttccgggtcc gcataatttc      480

gtggcgaacg atatcattgt tcataac                                          507


<210>  102
<211>  507
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Codon-optimized sequence DnaB_v26 encoding Synechocystis sp. 
       PCC6803 DnaB 6xHis-tagged non-ligating mini-intein (SEQ ID NO:2)

<400>  102
atggctatat ctggagatag tctaatttca ctagcgtcca ccggtaagcg cgtgtccatc       60

aaagacctgc tggacgagaa ggactttgaa atctgggcaa ttaatgagca gaccatgaaa      120

ctggaatccg cgaaagtgtc gcgcgtgttc tgcactggta agaagttggt ctacatcctg      180

aaaacccgtc tgggccgtac catcaaagcg accgccaatc atcgttttct gacgattgat      240

ggttggaaac gtttggacga actgtccctg aaagagcaca ttgcattgcc gcgtaaactt      300

gagagctcta gcttacagct ggccagcggc catcaccacc accaccatgg tggtagcggc      360

tcatccccgg agatcgagaa gctgtcgcaa agcgacatct attgggatag catcgtgagc      420

atcaccgaaa ccggtgtaga ggaagtattc gatctgaccg tcccgggtcc gcataacttt      480

gttgcaaatg atattattgt tcataac                                          507


<210>  103
<211>  507
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Codon-optimized sequence DnaB_v27 encoding Synechocystis sp. 
       PCC6803 DnaB 6xHis-tagged non-ligating mini-intein (SEQ ID NO:2)


<220>
<221>  misc_feature
<222>  (6)..(6)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (12)..(12)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (15)..(15)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (24)..(24)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (30)..(30)
<223>  n is a, c, g, or t

<400>  103
atggcnatht cnggngayag yctnathtcn cttgcttcca cagggaagcg cgtatctatt       60

aaagatttgt tggacgaaaa agattttgaa atttgggcta tcaacgagca aaccatgaag      120

ctggaatctg caaaggtcag ccgcgtcttc tgcacaggta aaaaacttgt ttacattctt      180

aaaacgcgtc tgggtcgtac aatcaaggcg acagccaacc atcgcttttt aaccattgat      240

ggttggaagc gtctggatga gttgtctctg aaggagcaca tcgcgcttcc tcgtaaactg      300

gagtcgtcta gtttgcaatt ggcctccggg caccatcacc atcatcatgg ggggtcgggg      360

tcttcgccgg agatcgaaaa gttgtcccag tccgacatct actgggattc gatcgttagc      420

atcactgaga caggagtgga agaggtgttc gacttaacgg tgcctggacc ccataacttt      480

gtggcgaacg atatcatcgt tcacaac                                          507


<210>  104
<211>  507
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Codon-optimized sequence DnaB_v28 encoding Synechocystis sp. 
       PCC6803 DnaB 6xHis-tagged non-ligating mini-intein (SEQ ID NO:2)

<400>  104
atggccatat ctggtgactc gctgatcagt cttgcttcca cagggaagcg cgtatctatt       60

aaagatttgt tggacgaaaa agattttgaa atttgggcta tcaacgagca aaccatgaag      120

ctggaatctg caaaggtcag ccgcgtcttc tgcacaggta aaaaacttgt ttacattctt      180

aaaacgcgtc tgggtcgtac aatcaaggcg acagccaacc atcgcttttt aaccattgat      240

ggttggaagc gtctggatga gttgtctctg aaggagcaca tcgcgcttcc tcgtaaactg      300

gagtcgtcta gtttgcaatt ggcctccggg caccatcacc atcatcatgg ggggtcgggg      360

tcttcgccgg agatcgaaaa gttgtcccag tccgacatct actgggattc gatcgttagc      420

atcactgaga caggagtgga agaggtgttc gacttaacgg tgcctggacc ccataacttt      480

gtggcgaacg atatcatcgt tcacaac                                          507


<210>  105
<211>  507
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Codon-optimized sequence DnaB_v29 encoding Synechocystis sp. 
       PCC6803 DnaB 6xHis-tagged non-ligating mini-intein (SEQ ID NO:2)

<400>  105
atggctattt cgggcgattc actgataagt cttgcttcca cagggaagcg cgtatctatt       60

aaagatttgt tggacgaaaa agattttgaa atttgggcta tcaacgagca aaccatgaag      120

ctggaatctg caaaggtcag ccgcgtcttc tgcacaggta aaaaacttgt ttacattctt      180

aaaacgcgtc tgggtcgtac aatcaaggcg acagccaacc atcgcttttt aaccattgat      240

ggttggaagc gtctggatga gttgtctctg aaggagcaca tcgcgcttcc tcgtaaactg      300

gagtcgtcta gtttgcaatt ggcctccggg caccatcacc atcatcatgg ggggtcgggg      360

tcttcgccgg agatcgaaaa gttgtcccag tccgacatct actgggattc gatcgttagc      420

atcactgaga caggagtgga agaggtgttc gacttaacgg tgcctggacc ccataacttt      480

gtggcgaacg atatcatcgt tcacaac                                          507


<210>  106
<211>  507
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Codon-optimized sequence DnaB_v30 encoding Synechocystis sp. 
       PCC6803 DnaB 6xHis-tagged non-ligating mini-intein (SEQ ID NO:2)


<220>
<221>  misc_feature
<222>  (6)..(6)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (15)..(15)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (21)..(21)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (24)..(24)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (30)..(30)
<223>  n is a, c, g, or t

<400>  106
atggcnatha gyggngaytc nctnathtcn ttggcttcta ccggaaagcg cgtaagcatc       60

aaagacctgc ttgatgagaa agatttcgaa atctgggcga ttaacgagca aactatgaaa      120

ttggagtctg ctaaggtaag tcgcgtattc tgcactggga aaaagttagt ttatattttg      180

aaaacacgtt tagggcgcac tattaaggca acagcaaatc accgctttct gaccattgat      240

ggttggaaac gtctggatga gcttagtctt aaagaacaca tcgctttgcc tcgcaaactt      300

gaatcgtcat cgcttcaact ggcgtcaggc catcatcatc accatcatgg gggatcgggt      360

tcatctcccg agattgagaa actttcccag agcgacatct attgggatag tatcgtgagt      420

attactgaaa cgggggtgga ggaggtgttc gacttgaccg tccctggtcc tcataacttt      480

gttgcgaacg atattattgt tcacaac                                          507


<210>  107
<211>  507
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Codon-optimized sequence DnaB_v31 encoding Synechocystis sp. 
       PCC6803 DnaB 6xHis-tagged non-ligating mini-intein (SEQ ID NO:2)


<220>
<221>  misc_feature
<222>  (6)..(6)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (12)..(12)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (15)..(15)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (24)..(24)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (30)..(30)
<223>  n is a, c, g, or t

<400>  107
atggcnatht cnggngayag yctnathtcn cttgcttcca cagggaagcg cgtatctatt       60

aaagatttgt tggacgaaaa agattttgaa atttgggcta tcaacgagca aaccatgaag      120

ctggaatctg caaaggtcag ccgcgtcttc tgcacaggta aaaaacttgt ttacattctt      180

aaaacgcgtc tgggtcgtac aatcaaggcg acagccaacc atcgcttttt aaccattgat      240

ggttggaagc gtctggatga gttgtctctg aaggagcaca tcgcgcttcc tcgtaaactg      300

gagtcgtcta gtttgcaatt ggcctccggg caccatcacc atcatcatgg ggggtcgggg      360

tcttcgccgg agatcgaaaa gttgtcccag tccgacatct actgggattc gatcgttagc      420

atcactgaga caggagtgga agaggtgttc gacttaacgg tgcctggacc ccataacttt      480

gtggcgaacg atatcatcgt tcacaac                                          507


<210>  108
<211>  507
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Codon-optimized sequence DnaB_v32encoding Synechocystis sp. 
       PCC6803 DnaB 6xHis-tagged non-ligating mini-intein (SEQ ID NO:2)

<400>  108
atggcaatat ctggtgattc actcatctcc ttggcttcta ccggaaagcg cgtaagcatc       60

aaagacctgc ttgatgagaa agatttcgaa atctgggcga ttaacgagca aactatgaaa      120

ttggagtctg ctaaggtaag tcgcgtattc tgcactggga aaaagttagt ttatattttg      180

aaaacacgtt tagggcgcac tattaaggca acagcaaatc accgctttct gaccattgat      240

ggttggaaac gtctggatga gcttagtctt aaagaacaca tcgctttgcc tcgcaaactt      300

gaatcgtcat cgcttcaact ggcgtcaggc catcatcatc accatcatgg gggatcgggt      360

tcatctcccg agattgagaa actttcccag agcgacatct attgggatag tatcgtgagt      420

attactgaaa cgggggtgga ggaggtgttc gacttgaccg tccctggtcc tcataacttt      480

gttgcgaacg atattattgt tcacaac                                          507


<210>  109
<211>  507
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Codon-optimized sequence DnaB_v33 encoding Synechocystis sp. 
       PCC6803 DnaB 6xHis-tagged non-ligating mini-intein (SEQ ID NO:2)

<400>  109
atggcaatct cgggagattc actcatttca ttggcttcta ccggaaagcg cgtaagcatc       60

aaagacctgc ttgatgagaa agatttcgaa atctgggcga ttaacgagca aactatgaaa      120

ttggagtctg ctaaggtaag tcgcgtattc tgcactggga aaaagttagt ttatattttg      180

aaaacacgtt tagggcgcac tattaaggca acagcaaatc accgctttct gaccattgat      240

ggttggaaac gtctggatga gcttagtctt aaagaacaca tcgctttgcc tcgcaaactt      300

gaatcgtcat cgcttcaact ggcgtcaggc catcatcatc accatcatgg gggatcgggt      360

tcatctcccg agattgagaa actttcccag agcgacatct attgggatag tatcgtgagt      420

attactgaaa cgggggtgga ggaggtgttc gacttgaccg tccctggtcc tcataacttt      480

gttgcgaacg atattattgt tcacaac                                          507


<210>  110
<211>  507
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Codon-optimized sequence DnaB_v34 encoding Synechocystis sp. 
       PCC6803 DnaB 6xHis-tagged non-ligating mini-intein (SEQ ID NO:2)

<400>  110
atggccattt ctggtgacag tctcatttct ctagcgtcca ccggtaagcg cgtgtccatc       60

aaagacctgc tggacgagaa ggactttgaa atctgggcaa ttaatgagca gaccatgaaa      120

ctggaatccg cgaaagtgtc gcgcgtgttc tgcactggta agaagttggt ctacatcctg      180

aaaacccgtc tgggccgtac catcaaagcg accgccaatc atcgttttct gacgattgat      240

ggttggaaac gtttggacga actgtccctg aaagagcaca ttgcattgcc gcgtaaactt      300

gagagctcta gcttacagct ggccagcggc catcaccacc accaccatgg tggtagcggc      360

tcatccccgg agatcgagaa gctgtcgcaa agcgacatct attgggatag catcgtgagc      420

atcaccgaaa ccggtgtaga ggaagtattc gatctgaccg tcccgggtcc gcataacttt      480

gttgcaaatg atattattgt tcataac                                          507


<210>  111
<211>  507
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Codon-optimized sequence DnaB_v35 encoding Synechocystis sp. 
       PCC6803 DnaB 6xHis-tagged non-ligating mini-intein (SEQ ID NO:2)

<400>  111
atggctatct ctggggatag tctaatatct ctagcgtcca ccggtaagcg cgtgtccatc       60

aaagacctgc tggacgagaa ggactttgaa atctgggcaa ttaatgagca gaccatgaaa      120

ctggaatccg cgaaagtgtc gcgcgtgttc tgcactggta agaagttggt ctacatcctg      180

aaaacccgtc tgggccgtac catcaaagcg accgccaatc atcgttttct gacgattgat      240

ggttggaaac gtttggacga actgtccctg aaagagcaca ttgcattgcc gcgtaaactt      300

gagagctcta gcttacagct ggccagcggc catcaccacc accaccatgg tggtagcggc      360

tcatccccgg agatcgagaa gctgtcgcaa agcgacatct attgggatag catcgtgagc      420

atcaccgaaa ccggtgtaga ggaagtattc gatctgaccg tcccgggtcc gcataacttt      480

gttgcaaatg atattattgt tcataac                                          507


<210>  112
<211>  507
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Codon-optimized sequence DnaB_v36 encoding Synechocystis sp. 
       PCC6803 DnaB 6xHis-tagged non-ligating mini-intein (SEQ ID NO:2)

<400>  112
atggcaatat caggagatag tctaatctct ctagcgtcca ccggtaagcg cgtgtccatc       60

aaagacctgc tggacgagaa ggactttgaa atctgggcaa ttaatgagca gaccatgaaa      120

ctggaatccg cgaaagtgtc gcgcgtgttc tgcactggta agaagttggt ctacatcctg      180

aaaacccgtc tgggccgtac catcaaagcg accgccaatc atcgttttct gacgattgat      240

ggttggaaac gtttggacga actgtccctg aaagagcaca ttgcattgcc gcgtaaactt      300

gagagctcta gcttacagct ggccagcggc catcaccacc accaccatgg tggtagcggc      360

tcatccccgg agatcgagaa gctgtcgcaa agcgacatct attgggatag catcgtgagc      420

atcaccgaaa ccggtgtaga ggaagtattc gatctgaccg tcccgggtcc gcataacttt      480

gttgcaaatg atattattgt tcataac                                          507


<210>  113
<211>  507
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Codon-optimized sequence DnaB_v37 encoding Synechocystis sp. 
       PCC6803 DnaB 6xHis-tagged non-ligating mini-intein (SEQ ID NO:2)

<400>  113
atggctattt ctggtgacag tcttatttct ctagcgtcca ccggtaagcg cgtgtccatc       60

aaagacctgc tggacgagaa ggactttgaa atctgggcaa ttaatgagca gaccatgaaa      120

ctggaatccg cgaaagtgtc gcgcgtgttc tgcactggta agaagttggt ctacatcctg      180

aaaacccgtc tgggccgtac catcaaagcg accgccaatc atcgttttct gacgattgat      240

ggttggaaac gtttggacga actgtccctg aaagagcaca ttgcattgcc gcgtaaactt      300

gagagctcta gcttacagct ggccagcggc catcaccacc accaccatgg tggtagcggc      360

tcatccccgg agatcgagaa gctgtcgcaa agcgacatct attgggatag catcgtgagc      420

atcaccgaaa ccggtgtaga ggaagtattc gatctgaccg tcccgggtcc gcataacttt      480

gttgcaaatg atattattgt tcataac                                          507


<210>  114
<211>  507
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Codon-optimized sequence DnaB_v38 encoding Synechocystis sp. 
       PCC6803 DnaB 6xHis-tagged non-ligating mini-intein (SEQ ID NO:2)

<400>  114
atggctattt ccggagatag tctaatctcg ctagcgtcca ccggtaagcg cgtgtccatc       60

aaagacctgc tggacgagaa ggactttgaa atctgggcaa ttaatgagca gaccatgaaa      120

ctggaatccg cgaaagtgtc gcgcgtgttc tgcactggta agaagttggt ctacatcctg      180

aaaacccgtc tgggccgtac catcaaagcg accgccaatc atcgttttct gacgattgat      240

ggttggaaac gtttggacga actgtccctg aaagagcaca ttgcattgcc gcgtaaactt      300

gagagctcta gcttacagct ggccagcggc catcaccacc accaccatgg tggtagcggc      360

tcatccccgg agatcgagaa gctgtcgcaa agcgacatct attgggatag catcgtgagc      420

atcaccgaaa ccggtgtaga ggaagtattc gatctgaccg tcccgggtcc gcataacttt      480

gttgcaaatg atattattgt tcataac                                          507


<210>  115
<211>  507
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Codon-optimized sequence DnaB_v39 encoding Synechocystis sp. 
       PCC6803 DnaB 6xHis-tagged non-ligating mini-intein (SEQ ID NO:2)

<400>  115
atggcaatct ctggcgatag ccttatatca ctagcgtcca ccggtaagcg cgtgtccatc       60

aaagacctgc tggacgagaa ggactttgaa atctgggcaa ttaatgagca gaccatgaaa      120

ctggaatccg cgaaagtgtc gcgcgtgttc tgcactggta agaagttggt ctacatcctg      180

aaaacccgtc tgggccgtac catcaaagcg accgccaatc atcgttttct gacgattgat      240

ggttggaaac gtttggacga actgtccctg aaagagcaca ttgcattgcc gcgtaaactt      300

gagagctcta gcttacagct ggccagcggc catcaccacc accaccatgg tggtagcggc      360

tcatccccgg agatcgagaa gctgtcgcaa agcgacatct attgggatag catcgtgagc      420

atcaccgaaa ccggtgtaga ggaagtattc gatctgaccg tcccgggtcc gcataacttt      480

gttgcaaatg atattattgt tcataac                                          507


<210>  116
<211>  507
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Codon-optimized sequence DnaB_v40 encoding Synechocystis sp. 
       PCC6803 DnaB 6xHis-tagged non-ligating mini-intein (SEQ ID NO:2)


<220>
<221>  misc_feature
<222>  (6)..(6)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (12)..(12)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (15)..(15)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (21)..(21)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (24)..(24)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (33)..(33)
<223>  n is a, c, g, or t

<400>  116
atggcnatht cnggngaytc nctnatyagy ctngcttcca cagggaagcg cgtatctatt       60

aaagatttgt tggacgaaaa agattttgaa atttgggcta tcaacgagca aaccatgaag      120

ctggaatctg caaaggtcag ccgcgtcttc tgcacaggta aaaaacttgt ttacattctt      180

aaaacgcgtc tgggtcgtac aatcaaggcg acagccaacc atcgcttttt aaccattgat      240

ggttggaagc gtctggatga gttgtctctg aaggagcaca tcgcgcttcc tcgtaaactg      300

gagtcgtcta gtttgcaatt ggcctccggg caccatcacc atcatcatgg ggggtcgggg      360

tcttcgccgg agatcgaaaa gttgtcccag tccgacatct actgggattc gatcgttagc      420

atcactgaga caggagtgga agaggtgttc gacttaacgg tgcctggacc ccataacttt      480

gtggcgaacg atatcatcgt tcacaac                                          507


<210>  117
<211>  507
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Codon-optimized sequence DnaB_v41 encoding Synechocystis sp. 
       PCC6803 DnaB 6xHis-tagged non-ligating mini-intein (SEQ ID NO:2)


<220>
<221>  misc_feature
<222>  (6)..(6)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (12)..(12)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (15)..(15)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (21)..(21)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (24)..(24)
<223>  n is a, c, g, or t

<400>  117
atggcnatht cnggngaytc nctnathagy ttrgcttcta ccggaaagcg cgtaagcatc       60

aaagacctgc ttgatgagaa agatttcgaa atctgggcga ttaacgagca aactatgaaa      120

ttggagtctg ctaaggtaag tcgcgtattc tgcactggga aaaagttagt ttatattttg      180

aaaacacgtt tagggcgcac tattaaggca acagcaaatc accgctttct gaccattgat      240

ggttggaaac gtctggatga gcttagtctt aaagaacaca tcgctttgcc tcgcaaactt      300

gaatcgtcat cgcttcaact ggcgtcaggc catcatcatc accatcatgg gggatcgggt      360

tcatctcccg agattgagaa actttcccag agcgacatct attgggatag tatcgtgagt      420

attactgaaa cgggggtgga ggaggtgttc gacttgaccg tccctggtcc tcataacttt      480

gttgcgaacg atattattgt tcacaac                                          507


<210>  118
<211>  507
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Codon-optimized sequence DnaB_v42 encoding Synechocystis sp. 
       PCC6803 DnaB 6xHis-tagged non-ligating mini-intein (SEQ ID NO:2)

<400>  118
atggccattt ctggtgattc ccttatcagt ctcgcttcca cagggaagcg cgtatctatt       60

aaagatttgt tggacgaaaa agattttgaa atttgggcta tcaacgagca aaccatgaag      120

ctggaatctg caaaggtcag ccgcgtcttc tgcacaggta aaaaacttgt ttacattctt      180

aaaacgcgtc tgggtcgtac aatcaaggcg acagccaacc atcgcttttt aaccattgat      240

ggttggaagc gtctggatga gttgtctctg aaggagcaca tcgcgcttcc tcgtaaactg      300

gagtcgtcta gtttgcaatt ggcctccggg caccatcacc atcatcatgg ggggtcgggg      360

tcttcgccgg agatcgaaaa gttgtcccag tccgacatct actgggattc gatcgttagc      420

atcactgaga caggagtgga agaggtgttc gacttaacgg tgcctggacc ccataacttt      480

gtggcgaacg atatcatcgt tcacaac                                          507


<210>  119
<211>  507
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Codon-optimized sequence DnaB_v43 encoding Synechocystis sp. 
       PCC6803 DnaB 6xHis-tagged non-ligating mini-intein (SEQ ID NO:2)

<400>  119
atggccatat ctggcgattc ccttattagt ttagcttcta ccggaaagcg cgtaagcatc       60

aaagacctgc ttgatgagaa agatttcgaa atctgggcga ttaacgagca aactatgaaa      120

ttggagtctg ctaaggtaag tcgcgtattc tgcactggga aaaagttagt ttatattttg      180

aaaacacgtt tagggcgcac tattaaggca acagcaaatc accgctttct gaccattgat      240

ggttggaaac gtctggatga gcttagtctt aaagaacaca tcgctttgcc tcgcaaactt      300

gaatcgtcat cgcttcaact ggcgtcaggc catcatcatc accatcatgg gggatcgggt      360

tcatctcccg agattgagaa actttcccag agcgacatct attgggatag tatcgtgagt      420

attactgaaa cgggggtgga ggaggtgttc gacttgaccg tccctggtcc tcataacttt      480

gttgcgaacg atattattgt tcacaac                                          507


<210>  120
<211>  169
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synechocystis sp. PCC6803, DnaB; 4xHisKA-tagged mini-intein

<400>  120

Met Ala Ile Ser Gly Asp Ser Leu Ile Ser Leu Ala Ser Thr Gly Lys 
1               5                   10                  15      


Arg Val Ser Ile Lys Asp Leu Leu Asp Glu Lys Asp Phe Glu Ile Trp 
            20                  25                  30          


Ala Ile Asn Glu Gln Thr Met Lys Leu Glu Ser Ala Lys Val Ser Arg 
        35                  40                  45              


Val Phe Cys Thr Gly Lys Lys Leu Val Tyr Ile Leu Lys Thr Arg Leu 
    50                  55                  60                  


Gly Arg Thr Ile Lys Ala Thr Ala Asn His Arg Phe Leu Thr Ile Asp 
65                  70                  75                  80  


Gly Trp Lys Arg Leu Asp Glu Leu Ser Leu Lys Glu His Ile Ala Leu 
                85                  90                  95      


Pro Arg Lys Leu Glu Ser Ser Ser Leu Gln Leu Ala Ser Gly His His 
            100                 105                 110         


His His Lys Ala Gly Gly Ser Gly Ser Ser Pro Glu Ile Glu Lys Leu 
        115                 120                 125             


Ser Gln Ser Asp Ile Tyr Trp Asp Ser Ile Val Ser Ile Thr Glu Thr 
    130                 135                 140                 


Gly Val Glu Glu Val Phe Asp Leu Thr Val Pro Gly Pro His Asn Phe 
145                 150                 155                 160 


Val Ala Asn Asp Ile Ile Val His Asn 
                165                 


<210>  121
<211>  169
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synechocystis sp. PCC6803, DnaB; 4xHisKH-tagged mini-intein

<400>  121

Met Ala Ile Ser Gly Asp Ser Leu Ile Ser Leu Ala Ser Thr Gly Lys 
1               5                   10                  15      


Arg Val Ser Ile Lys Asp Leu Leu Asp Glu Lys Asp Phe Glu Ile Trp 
            20                  25                  30          


Ala Ile Asn Glu Gln Thr Met Lys Leu Glu Ser Ala Lys Val Ser Arg 
        35                  40                  45              


Val Phe Cys Thr Gly Lys Lys Leu Val Tyr Ile Leu Lys Thr Arg Leu 
    50                  55                  60                  


Gly Arg Thr Ile Lys Ala Thr Ala Asn His Arg Phe Leu Thr Ile Asp 
65                  70                  75                  80  


Gly Trp Lys Arg Leu Asp Glu Leu Ser Leu Lys Glu His Ile Ala Leu 
                85                  90                  95      


Pro Arg Lys Leu Glu Ser Ser Ser Leu Gln Leu Ala Ser Gly His His 
            100                 105                 110         


His His Lys His Gly Gly Ser Gly Ser Ser Pro Glu Ile Glu Lys Leu 
        115                 120                 125             


Ser Gln Ser Asp Ile Tyr Trp Asp Ser Ile Val Ser Ile Thr Glu Thr 
    130                 135                 140                 


Gly Val Glu Glu Val Phe Asp Leu Thr Val Pro Gly Pro His Asn Phe 
145                 150                 155                 160 


Val Ala Asn Asp Ile Ile Val His Asn 
                165                 


<210>  122
<211>  169
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synechocystis sp. PCC6803, DnaB; 4xHisKS-tagged mini-intein

<400>  122

Met Ala Ile Ser Gly Asp Ser Leu Ile Ser Leu Ala Ser Thr Gly Lys 
1               5                   10                  15      


Arg Val Ser Ile Lys Asp Leu Leu Asp Glu Lys Asp Phe Glu Ile Trp 
            20                  25                  30          


Ala Ile Asn Glu Gln Thr Met Lys Leu Glu Ser Ala Lys Val Ser Arg 
        35                  40                  45              


Val Phe Cys Thr Gly Lys Lys Leu Val Tyr Ile Leu Lys Thr Arg Leu 
    50                  55                  60                  


Gly Arg Thr Ile Lys Ala Thr Ala Asn His Arg Phe Leu Thr Ile Asp 
65                  70                  75                  80  


Gly Trp Lys Arg Leu Asp Glu Leu Ser Leu Lys Glu His Ile Ala Leu 
                85                  90                  95      


Pro Arg Lys Leu Glu Ser Ser Ser Leu Gln Leu Ala Ser Gly His His 
            100                 105                 110         


His His Lys Ser Gly Gly Ser Gly Ser Ser Pro Glu Ile Glu Lys Leu 
        115                 120                 125             


Ser Gln Ser Asp Ile Tyr Trp Asp Ser Ile Val Ser Ile Thr Glu Thr 
    130                 135                 140                 


Gly Val Glu Glu Val Phe Asp Leu Thr Val Pro Gly Pro His Asn Phe 
145                 150                 155                 160 


Val Ala Asn Asp Ile Ile Val His Asn 
                165                 


<210>  123
<211>  169
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synechocystis sp. PCC6803, DnaB; 4xHisKT-tagged mini-intein

<400>  123

Met Ala Ile Ser Gly Asp Ser Leu Ile Ser Leu Ala Ser Thr Gly Lys 
1               5                   10                  15      


Arg Val Ser Ile Lys Asp Leu Leu Asp Glu Lys Asp Phe Glu Ile Trp 
            20                  25                  30          


Ala Ile Asn Glu Gln Thr Met Lys Leu Glu Ser Ala Lys Val Ser Arg 
        35                  40                  45              


Val Phe Cys Thr Gly Lys Lys Leu Val Tyr Ile Leu Lys Thr Arg Leu 
    50                  55                  60                  


Gly Arg Thr Ile Lys Ala Thr Ala Asn His Arg Phe Leu Thr Ile Asp 
65                  70                  75                  80  


Gly Trp Lys Arg Leu Asp Glu Leu Ser Leu Lys Glu His Ile Ala Leu 
                85                  90                  95      


Pro Arg Lys Leu Glu Ser Ser Ser Leu Gln Leu Ala Ser Gly His His 
            100                 105                 110         


His His Lys Thr Gly Gly Ser Gly Ser Ser Pro Glu Ile Glu Lys Leu 
        115                 120                 125             


Ser Gln Ser Asp Ile Tyr Trp Asp Ser Ile Val Ser Ile Thr Glu Thr 
    130                 135                 140                 


Gly Val Glu Glu Val Phe Asp Leu Thr Val Pro Gly Pro His Asn Phe 
145                 150                 155                 160 


Val Ala Asn Asp Ile Ile Val His Asn 
                165                 


<210>  124
<211>  155
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synechocystis sp. PCC6803, DnaB; 6xHis-tagged mini-intein variant
       M86

<400>  124

Met Ala Ile Ser Gly Asp Ser Leu Ile Ser Leu Ala Ser Thr Gly Lys 
1               5                   10                  15      


Arg Val Pro Ile Lys Asp Leu Leu Gly Glu Lys Asp Phe Glu Ile Trp 
            20                  25                  30          


Ala Ile Asn Glu Gln Thr Met Lys Leu Glu Ser Ala Lys Val Ser Arg 
        35                  40                  45              


Val Phe Cys Thr Gly Lys Lys Leu Val Tyr Thr Leu Lys Thr Arg Leu 
    50                  55                  60                  


Gly Arg Thr Ile Lys Ala Thr Ala Asn His Arg Phe Leu Thr Ile Asp 
65                  70                  75                  80  


Gly Trp Lys Arg Leu Asp Glu Leu Ser Leu Lys Glu His Ile Ala Leu 
                85                  90                  95      


Pro Arg Lys Leu Glu Ser Ser Ser Leu Gln Leu Ala Pro Glu Ile Glu 
            100                 105                 110         


Lys Leu Pro Gln Ser Asp Ile Tyr Trp Asp Pro Ile Val Ser Ile Thr 
        115                 120                 125             


Glu Thr Gly Val Glu Glu Val Phe Asp Leu Thr Val Pro Gly Leu Arg 
    130                 135                 140                 


Asn Phe Val Ala Asn Asp Ile Ile Val His Asn 
145                 150                 155 


