                         SEQUENCE LISTING

<110>  PharmaZell GmbH
        ,
 
<120>  Enantioselective Chemo-enzymatic Synthesis of optically active 
       Amino amide compounds

<130>  M/60205-PCT

<160>  145   

<170>  PatentIn version 3.5

<210>  1
<211>  657
<212>  DNA
<213>  Comamonas testosteroni


<220>
<221>  misc_feature
<223>  CtNHase beta-subunit_AAU87543.1

<220>
<221>  CDS
<222>  (1)..(657)

<400>  1
atg aat ggc att cac gat act ggg gga gca cat ggt tat ggg ccg gtt         48
Met Asn Gly Ile His Asp Thr Gly Gly Ala His Gly Tyr Gly Pro Val           
1               5                   10                  15                

tac aga gaa ccg aac gaa ccc gtc ttt cgc tac gac tgg gaa aaa acg         96
Tyr Arg Glu Pro Asn Glu Pro Val Phe Arg Tyr Asp Trp Glu Lys Thr           
            20                  25                  30                    

gtc atg tcc ctg ttc ccg gcg ctg ttc gcc aac ggc aac ttc aac ctc        144
Val Met Ser Leu Phe Pro Ala Leu Phe Ala Asn Gly Asn Phe Asn Leu           
        35                  40                  45                        

gat gag ttt cga cac ggc atc gag cgc atg aac ccc atc gac tac ctg        192
Asp Glu Phe Arg His Gly Ile Glu Arg Met Asn Pro Ile Asp Tyr Leu           
    50                  55                  60                            

aag gga acc tac tac gaa cac tgg atc cat tcc atc gaa acc ttg ctg        240
Lys Gly Thr Tyr Tyr Glu His Trp Ile His Ser Ile Glu Thr Leu Leu           
65                  70                  75                  80            

gtc gaa aag ggt gtg ctc acg gca acg gaa ctc gcg acc ggc aag gca        288
Val Glu Lys Gly Val Leu Thr Ala Thr Glu Leu Ala Thr Gly Lys Ala           
                85                  90                  95                

tct ggc aag aca gcg aca ccg gtg ctg acg ccg gcc atc gtg gac gga        336
Ser Gly Lys Thr Ala Thr Pro Val Leu Thr Pro Ala Ile Val Asp Gly           
            100                 105                 110                   

ctg ctc agc acc ggg gct tct gcc gcc cgg gag gag ggt gcg cgg gcg        384
Leu Leu Ser Thr Gly Ala Ser Ala Ala Arg Glu Glu Gly Ala Arg Ala           
        115                 120                 125                       

cgg ttc gct gtg ggg gac aag gtt cgc gtc ctc aac aag aac ccg gtg        432
Arg Phe Ala Val Gly Asp Lys Val Arg Val Leu Asn Lys Asn Pro Val           
    130                 135                 140                           

ggc cat acc cgc atg ccg cgc tac acg cgg ggc aaa gtg ggg aca gtg        480
Gly His Thr Arg Met Pro Arg Tyr Thr Arg Gly Lys Val Gly Thr Val           
145                 150                 155                 160           

gtc atc gac cat ggt gtg ttc gtg acg ccg gac acc gcg gca cac gga        528
Val Ile Asp His Gly Val Phe Val Thr Pro Asp Thr Ala Ala His Gly           
                165                 170                 175               

aag ggc gag cac ccc cag cac gtt tac acc gtg agt ttc acg tcg gtc        576
Lys Gly Glu His Pro Gln His Val Tyr Thr Val Ser Phe Thr Ser Val           
            180                 185                 190                   

gaa ctg tgg ggg caa gac gcc tcc tcg ccg aag gac acg att cgc gtc        624
Glu Leu Trp Gly Gln Asp Ala Ser Ser Pro Lys Asp Thr Ile Arg Val           
        195                 200                 205                       

gac ttg tgg gat gac tac ctg gag cca gcg tga                            657
Asp Leu Trp Asp Asp Tyr Leu Glu Pro Ala                                   
    210                 215                                               


<210>  2
<211>  218
<212>  PRT
<213>  Comamonas testosteroni

<400>  2

Met Asn Gly Ile His Asp Thr Gly Gly Ala His Gly Tyr Gly Pro Val 
1               5                   10                  15      


Tyr Arg Glu Pro Asn Glu Pro Val Phe Arg Tyr Asp Trp Glu Lys Thr 
            20                  25                  30          


Val Met Ser Leu Phe Pro Ala Leu Phe Ala Asn Gly Asn Phe Asn Leu 
        35                  40                  45              


Asp Glu Phe Arg His Gly Ile Glu Arg Met Asn Pro Ile Asp Tyr Leu 
    50                  55                  60                  


Lys Gly Thr Tyr Tyr Glu His Trp Ile His Ser Ile Glu Thr Leu Leu 
65                  70                  75                  80  


Val Glu Lys Gly Val Leu Thr Ala Thr Glu Leu Ala Thr Gly Lys Ala 
                85                  90                  95      


Ser Gly Lys Thr Ala Thr Pro Val Leu Thr Pro Ala Ile Val Asp Gly 
            100                 105                 110         


Leu Leu Ser Thr Gly Ala Ser Ala Ala Arg Glu Glu Gly Ala Arg Ala 
        115                 120                 125             


Arg Phe Ala Val Gly Asp Lys Val Arg Val Leu Asn Lys Asn Pro Val 
    130                 135                 140                 


Gly His Thr Arg Met Pro Arg Tyr Thr Arg Gly Lys Val Gly Thr Val 
145                 150                 155                 160 


Val Ile Asp His Gly Val Phe Val Thr Pro Asp Thr Ala Ala His Gly 
                165                 170                 175     


Lys Gly Glu His Pro Gln His Val Tyr Thr Val Ser Phe Thr Ser Val 
            180                 185                 190         


Glu Leu Trp Gly Gln Asp Ala Ser Ser Pro Lys Asp Thr Ile Arg Val 
        195                 200                 205             


Asp Leu Trp Asp Asp Tyr Leu Glu Pro Ala 
    210                 215             


<210>  3
<211>  654
<212>  DNA
<213>  Klebsiella oxytoca


<220>
<221>  misc_feature
<223>  KoNHase beta-subunit_OSY94201.1WP

<220>
<221>  CDS
<222>  (1)..(654)

<400>  3
atg aac ggg ata cat gat ctg ggg ggg atg cac ggc ctt ggc ccg atc         48
Met Asn Gly Ile His Asp Leu Gly Gly Met His Gly Leu Gly Pro Ile           
1               5                   10                  15                

cct acc gag gaa aac gag ccc tat ttc cat cat gag tgg gaa cgc cgg         96
Pro Thr Glu Glu Asn Glu Pro Tyr Phe His His Glu Trp Glu Arg Arg           
            20                  25                  30                    

gta ttt cct ctg ttc gcc tcg ttg ttc gtc ggc gga cat ttt aac gtc        144
Val Phe Pro Leu Phe Ala Ser Leu Phe Val Gly Gly His Phe Asn Val           
        35                  40                  45                        

gat gaa ttt cgc cac gcc atc gaa cgt atg gcg ccg acc gaa tat ttg        192
Asp Glu Phe Arg His Ala Ile Glu Arg Met Ala Pro Thr Glu Tyr Leu           
    50                  55                  60                            

cag tcg agc tac tac gag cac tgg ctg cat gca ttc gaa acg ctg ctg        240
Gln Ser Ser Tyr Tyr Glu His Trp Leu His Ala Phe Glu Thr Leu Leu           
65                  70                  75                  80            

ctg gca aag ggg gcg atc acc gtt gaa gaa ctg tgg ggt ggc gcg aag        288
Leu Ala Lys Gly Ala Ile Thr Val Glu Glu Leu Trp Gly Gly Ala Lys           
                85                  90                  95                

cct gcc cct tgc aag cct ggc aca cct gtg ctg acg cag gag atg gtg        336
Pro Ala Pro Cys Lys Pro Gly Thr Pro Val Leu Thr Gln Glu Met Val           
            100                 105                 110                   

tcg atg gtg gtc agc acc ggc ggg tct gct cgg gtc agt cac gat gtt        384
Ser Met Val Val Ser Thr Gly Gly Ser Ala Arg Val Ser His Asp Val           
        115                 120                 125                       

gcg ccc cgc ttc cgg gtg ggc gat tgg gta cga acg aaa aat ttc aac        432
Ala Pro Arg Phe Arg Val Gly Asp Trp Val Arg Thr Lys Asn Phe Asn           
    130                 135                 140                           

ccg acc acc cat acc cgc ctg cca cgc tac gca cgc gat aaa gtc ggt        480
Pro Thr Thr His Thr Arg Leu Pro Arg Tyr Ala Arg Asp Lys Val Gly           
145                 150                 155                 160           

cgc ata gag atc gct cac ggt gtg ttt atc acg cca gat act gcg gcg        528
Arg Ile Glu Ile Ala His Gly Val Phe Ile Thr Pro Asp Thr Ala Ala           
                165                 170                 175               

cac ggg ctg ggc gaa cat ccc cag cat gtt tac agc gtc agt ttc acc        576
His Gly Leu Gly Glu His Pro Gln His Val Tyr Ser Val Ser Phe Thr           
            180                 185                 190                   

gcg cag gcg ctg tgg gga gag ccg cgc cct gac aaa gtg ttc atc gat        624
Ala Gln Ala Leu Trp Gly Glu Pro Arg Pro Asp Lys Val Phe Ile Asp           
        195                 200                 205                       

ctg tgg gac gac tat ctg gag gaa gca taa                                654
Leu Trp Asp Asp Tyr Leu Glu Glu Ala                                       
    210                 215                                               


<210>  4
<211>  217
<212>  PRT
<213>  Klebsiella oxytoca

<400>  4

Met Asn Gly Ile His Asp Leu Gly Gly Met His Gly Leu Gly Pro Ile 
1               5                   10                  15      


Pro Thr Glu Glu Asn Glu Pro Tyr Phe His His Glu Trp Glu Arg Arg 
            20                  25                  30          


Val Phe Pro Leu Phe Ala Ser Leu Phe Val Gly Gly His Phe Asn Val 
        35                  40                  45              


Asp Glu Phe Arg His Ala Ile Glu Arg Met Ala Pro Thr Glu Tyr Leu 
    50                  55                  60                  


Gln Ser Ser Tyr Tyr Glu His Trp Leu His Ala Phe Glu Thr Leu Leu 
65                  70                  75                  80  


Leu Ala Lys Gly Ala Ile Thr Val Glu Glu Leu Trp Gly Gly Ala Lys 
                85                  90                  95      


Pro Ala Pro Cys Lys Pro Gly Thr Pro Val Leu Thr Gln Glu Met Val 
            100                 105                 110         


Ser Met Val Val Ser Thr Gly Gly Ser Ala Arg Val Ser His Asp Val 
        115                 120                 125             


Ala Pro Arg Phe Arg Val Gly Asp Trp Val Arg Thr Lys Asn Phe Asn 
    130                 135                 140                 


Pro Thr Thr His Thr Arg Leu Pro Arg Tyr Ala Arg Asp Lys Val Gly 
145                 150                 155                 160 


Arg Ile Glu Ile Ala His Gly Val Phe Ile Thr Pro Asp Thr Ala Ala 
                165                 170                 175     


His Gly Leu Gly Glu His Pro Gln His Val Tyr Ser Val Ser Phe Thr 
            180                 185                 190         


Ala Gln Ala Leu Trp Gly Glu Pro Arg Pro Asp Lys Val Phe Ile Asp 
        195                 200                 205             


Leu Trp Asp Asp Tyr Leu Glu Glu Ala 
    210                 215         


<210>  5
<211>  684
<212>  DNA
<213>  Nitriliruptor alkaliphilus


<220>
<221>  misc_feature
<223>  NaNHase beta-subunit_WP_052668588.1

<220>
<221>  CDS
<222>  (1)..(684)

<400>  5
atg aac ggc gtc cat gat ctt ggc ggc acc gac ggc ctc ggc ccg gtg         48
Met Asn Gly Val His Asp Leu Gly Gly Thr Asp Gly Leu Gly Pro Val           
1               5                   10                  15                

atc acc gag gag aac gag ccg gtc tgg cac agc gag tgg gag aag gcc         96
Ile Thr Glu Glu Asn Glu Pro Val Trp His Ser Glu Trp Glu Lys Ala           
            20                  25                  30                    

gtc ttc acg atg ttc ccc acc aac ttc gcc aag gga cat ttc aac gtc        144
Val Phe Thr Met Phe Pro Thr Asn Phe Ala Lys Gly His Phe Asn Val           
        35                  40                  45                        

gac tcg ttc cgc ttc ggg atc gag aag atc cat ccg gcg gac tac ctg        192
Asp Ser Phe Arg Phe Gly Ile Glu Lys Ile His Pro Ala Asp Tyr Leu           
    50                  55                  60                            

tcg tcc cgg tac tac gag cac tgg ctg cac tcc atc gag cat gcg gtg        240
Ser Ser Arg Tyr Tyr Glu His Trp Leu His Ser Ile Glu His Ala Val           
65                  70                  75                  80            

gtg gat cag ggc gtc gtc gac gcc gag gag ctg gag gcg cgc act cgt        288
Val Asp Gln Gly Val Val Asp Ala Glu Glu Leu Glu Ala Arg Thr Arg           
                85                  90                  95                

cac tac ctc gag aac ccc gac gcg ccg ttg ccg gac cgc cag gac cct        336
His Tyr Leu Glu Asn Pro Asp Ala Pro Leu Pro Asp Arg Gln Asp Pro           
            100                 105                 110                   

gat ctg gtg cca ctg gtg gag acg atc tcg cgg ggt ggt ggc tcc gca        384
Asp Leu Val Pro Leu Val Glu Thr Ile Ser Arg Gly Gly Gly Ser Ala           
        115                 120                 125                       

cgt cgc gag agc gac aag gcg ccg agg ttc gag gtc ggt gac cga gtc        432
Arg Arg Glu Ser Asp Lys Ala Pro Arg Phe Glu Val Gly Asp Arg Val           
    130                 135                 140                           

cgc gtc aag cgc gac gag gtg ccc cac ggc cac acg cgc cgc gcc cgc        480
Arg Val Lys Arg Asp Glu Val Pro His Gly His Thr Arg Arg Ala Arg           
145                 150                 155                 160           

tac gtg cag ggg cgc gaa ggc gtg atc gtc cag gcc cac ggg gcg ttc        528
Tyr Val Gln Gly Arg Glu Gly Val Ile Val Gln Ala His Gly Ala Phe           
                165                 170                 175               

atc tac ccg gac agc gca ggc aac ggc ggg ccg gag gac ccc gag cac        576
Ile Tyr Pro Asp Ser Ala Gly Asn Gly Gly Pro Glu Asp Pro Glu His           
            180                 185                 190                   

gtc tac acg atc ctg ttc gag gcc tcg cac ctg tgg ggc gaa cgg acg        624
Val Tyr Thr Ile Leu Phe Glu Ala Ser His Leu Trp Gly Glu Arg Thr           
        195                 200                 205                       

ggt gac tcc aac gga acc gtg acc ttc gac gcg tgg gag ccc tac ctc        672
Gly Asp Ser Asn Gly Thr Val Thr Phe Asp Ala Trp Glu Pro Tyr Leu           
    210                 215                 220                           

gag ctg gtc tga                                                        684
Glu Leu Val                                                               
225                                                                       


<210>  6
<211>  227
<212>  PRT
<213>  Nitriliruptor alkaliphilus

<400>  6

Met Asn Gly Val His Asp Leu Gly Gly Thr Asp Gly Leu Gly Pro Val 
1               5                   10                  15      


Ile Thr Glu Glu Asn Glu Pro Val Trp His Ser Glu Trp Glu Lys Ala 
            20                  25                  30          


Val Phe Thr Met Phe Pro Thr Asn Phe Ala Lys Gly His Phe Asn Val 
        35                  40                  45              


Asp Ser Phe Arg Phe Gly Ile Glu Lys Ile His Pro Ala Asp Tyr Leu 
    50                  55                  60                  


Ser Ser Arg Tyr Tyr Glu His Trp Leu His Ser Ile Glu His Ala Val 
65                  70                  75                  80  


Val Asp Gln Gly Val Val Asp Ala Glu Glu Leu Glu Ala Arg Thr Arg 
                85                  90                  95      


His Tyr Leu Glu Asn Pro Asp Ala Pro Leu Pro Asp Arg Gln Asp Pro 
            100                 105                 110         


Asp Leu Val Pro Leu Val Glu Thr Ile Ser Arg Gly Gly Gly Ser Ala 
        115                 120                 125             


Arg Arg Glu Ser Asp Lys Ala Pro Arg Phe Glu Val Gly Asp Arg Val 
    130                 135                 140                 


Arg Val Lys Arg Asp Glu Val Pro His Gly His Thr Arg Arg Ala Arg 
145                 150                 155                 160 


Tyr Val Gln Gly Arg Glu Gly Val Ile Val Gln Ala His Gly Ala Phe 
                165                 170                 175     


Ile Tyr Pro Asp Ser Ala Gly Asn Gly Gly Pro Glu Asp Pro Glu His 
            180                 185                 190         


Val Tyr Thr Ile Leu Phe Glu Ala Ser His Leu Trp Gly Glu Arg Thr 
        195                 200                 205             


Gly Asp Ser Asn Gly Thr Val Thr Phe Asp Ala Trp Glu Pro Tyr Leu 
    210                 215                 220                 


Glu Leu Val 
225         


<210>  7
<211>  654
<212>  DNA
<213>  Gordonia hydrophobica


<220>
<221>  misc_feature
<223>  GhNHase beta-subunit_WP_066163466.1

<220>
<221>  CDS
<222>  (1)..(654)

<400>  7
atg cac gga att cac gat ctc ggc ggc gtc cag aac ttc ggg ccg gtt         48
Met His Gly Ile His Asp Leu Gly Gly Val Gln Asn Phe Gly Pro Val           
1               5                   10                  15                

ccg cat cgg gtc aat gag tac ccc gac ggt ccg ttc cag acg gcc gag         96
Pro His Arg Val Asn Glu Tyr Pro Asp Gly Pro Phe Gln Thr Ala Glu           
            20                  25                  30                    

tac cac gag gac tgg gag cct ctg gcc tac ggt ctg ctg ttc gcc tgc        144
Tyr His Glu Asp Trp Glu Pro Leu Ala Tyr Gly Leu Leu Phe Ala Cys           
        35                  40                  45                        

gcc gac gcc gat cag ttc agc gtc gat cag cta cgg cac tcg atc gag        192
Ala Asp Ala Asp Gln Phe Ser Val Asp Gln Leu Arg His Ser Ile Glu           
    50                  55                  60                            

cgt atg gag ccg cgc gac tac atg acg agc agc tac ttc gaa cgc att        240
Arg Met Glu Pro Arg Asp Tyr Met Thr Ser Ser Tyr Phe Glu Arg Ile           
65                  70                  75                  80            

ctg gtc ggc acc gcc act ctg atg gtg gag aac ggc atg ctc acc cag        288
Leu Val Gly Thr Ala Thr Leu Met Val Glu Asn Gly Met Leu Thr Gln           
                85                  90                  95                

gaa gag ctc gaa gac ttg gcc ggc gga ccg ttt ccg ctc tcg aga cca        336
Glu Glu Leu Glu Asp Leu Ala Gly Gly Pro Phe Pro Leu Ser Arg Pro           
            100                 105                 110                   

gtt agc tcg gcc ggg cgg ccg gcg aat ccg aat ctg cag cag ttc gag        384
Val Ser Ser Ala Gly Arg Pro Ala Asn Pro Asn Leu Gln Gln Phe Glu           
        115                 120                 125                       

gtc ggc gac cgg gta cgg gtg gcg acc gaa cag gta ccc ggc cac att        432
Val Gly Asp Arg Val Arg Val Ala Thr Glu Gln Val Pro Gly His Ile           
    130                 135                 140                           

cgg gtg ccc gga tac tgc ttc ggc aag gag ggc gtg atc gac cac cgc        480
Arg Val Pro Gly Tyr Cys Phe Gly Lys Glu Gly Val Ile Asp His Arg           
145                 150                 155                 160           

acc gca cac gag tgg cgg ttc ccc gac gcg atc gga cac ggc cgc gac        528
Thr Ala His Glu Trp Arg Phe Pro Asp Ala Ile Gly His Gly Arg Asp           
                165                 170                 175               

gac ggt ggt tcc gaa ccc acc tac cac gtc cga ttc gat gcc acc gac        576
Asp Gly Gly Ser Glu Pro Thr Tyr His Val Arg Phe Asp Ala Thr Asp           
            180                 185                 190                   

gtc ttc ggc acc gac acc gag gcc gag tcc atc gtc gtc gac ctc ttc        624
Val Phe Gly Thr Asp Thr Glu Ala Glu Ser Ile Val Val Asp Leu Phe           
        195                 200                 205                       

ggc ggc tac ctg gag ccg gtg ccg gcc tga                                654
Gly Gly Tyr Leu Glu Pro Val Pro Ala                                       
    210                 215                                               


<210>  8
<211>  217
<212>  PRT
<213>  Gordonia hydrophobica

<400>  8

Met His Gly Ile His Asp Leu Gly Gly Val Gln Asn Phe Gly Pro Val 
1               5                   10                  15      


Pro His Arg Val Asn Glu Tyr Pro Asp Gly Pro Phe Gln Thr Ala Glu 
            20                  25                  30          


Tyr His Glu Asp Trp Glu Pro Leu Ala Tyr Gly Leu Leu Phe Ala Cys 
        35                  40                  45              


Ala Asp Ala Asp Gln Phe Ser Val Asp Gln Leu Arg His Ser Ile Glu 
    50                  55                  60                  


Arg Met Glu Pro Arg Asp Tyr Met Thr Ser Ser Tyr Phe Glu Arg Ile 
65                  70                  75                  80  


Leu Val Gly Thr Ala Thr Leu Met Val Glu Asn Gly Met Leu Thr Gln 
                85                  90                  95      


Glu Glu Leu Glu Asp Leu Ala Gly Gly Pro Phe Pro Leu Ser Arg Pro 
            100                 105                 110         


Val Ser Ser Ala Gly Arg Pro Ala Asn Pro Asn Leu Gln Gln Phe Glu 
        115                 120                 125             


Val Gly Asp Arg Val Arg Val Ala Thr Glu Gln Val Pro Gly His Ile 
    130                 135                 140                 


Arg Val Pro Gly Tyr Cys Phe Gly Lys Glu Gly Val Ile Asp His Arg 
145                 150                 155                 160 


Thr Ala His Glu Trp Arg Phe Pro Asp Ala Ile Gly His Gly Arg Asp 
                165                 170                 175     


Asp Gly Gly Ser Glu Pro Thr Tyr His Val Arg Phe Asp Ala Thr Asp 
            180                 185                 190         


Val Phe Gly Thr Asp Thr Glu Ala Glu Ser Ile Val Val Asp Leu Phe 
        195                 200                 205             


Gly Gly Tyr Leu Glu Pro Val Pro Ala 
    210                 215         


<210>  9
<211>  663
<212>  DNA
<213>  Pseudomonas marginalis


<220>
<221>  misc_feature
<223>  PmNHase beta-subunit_WP_074846644.1

<220>
<221>  CDS
<222>  (1)..(663)

<400>  9
atg gat ggc ttt cac gat ctc ggc ggt ttc caa ggc ttt gga aaa gtc         48
Met Asp Gly Phe His Asp Leu Gly Gly Phe Gln Gly Phe Gly Lys Val           
1               5                   10                  15                

cct cac acc atc aac agc ctg agc tac aaa cag gtg ttc aag cag gac         96
Pro His Thr Ile Asn Ser Leu Ser Tyr Lys Gln Val Phe Lys Gln Asp           
            20                  25                  30                    

tgg gag cat ctg gcc tac agc ttg atg ttc atc ggt gcc gac cac ttg        144
Trp Glu His Leu Ala Tyr Ser Leu Met Phe Ile Gly Ala Asp His Leu           
        35                  40                  45                        

aaa aag ttc agc gtg gac gaa gtg cgt cac gcc gtc gaa cgc ctg gat        192
Lys Lys Phe Ser Val Asp Glu Val Arg His Ala Val Glu Arg Leu Asp           
    50                  55                  60                            

gtg cgc cag cat gtc ggc acc cag tac tac gaa cgc tac gtc atc gcg        240
Val Arg Gln His Val Gly Thr Gln Tyr Tyr Glu Arg Tyr Val Ile Ala           
65                  70                  75                  80            

acc gcc acc ctg ctg gtc gaa acc ggc gtg atc acc cag gcg gag ctt        288
Thr Ala Thr Leu Leu Val Glu Thr Gly Val Ile Thr Gln Ala Glu Leu           
                85                  90                  95                

gat cag gcc ttg ggc tcc cac ttc aag ctg gcg aat ccc gcc cat gcc        336
Asp Gln Ala Leu Gly Ser His Phe Lys Leu Ala Asn Pro Ala His Ala           
            100                 105                 110                   

gag ggc cgc ccg gcg atc acg ggg cgg ccg ccc ttc gag gtg ggg gat        384
Glu Gly Arg Pro Ala Ile Thr Gly Arg Pro Pro Phe Glu Val Gly Asp           
        115                 120                 125                       

cgg gtg gtg gtg cga gac gaa tat gtg gct ggg cac atc cgc atg ccc        432
Arg Val Val Val Arg Asp Glu Tyr Val Ala Gly His Ile Arg Met Pro           
    130                 135                 140                           

gcc tac gtg cgc ggc aag gaa ggc gtg gtc ctg cac cgc acg tca gag        480
Ala Tyr Val Arg Gly Lys Glu Gly Val Val Leu His Arg Thr Ser Glu           
145                 150                 155                 160           

aaa tgg ccg ttc ccc gac gcg att ggg cat ggc gat gta agc gcc gcc        528
Lys Trp Pro Phe Pro Asp Ala Ile Gly His Gly Asp Val Ser Ala Ala           
                165                 170                 175               

cat caa ccc acc tac cac gtc gag ttt tcc gtg aaa gac ctg tgg ggg        576
His Gln Pro Thr Tyr His Val Glu Phe Ser Val Lys Asp Leu Trp Gly           
            180                 185                 190                   

gat gcg gcc gat gag ggt ttt gtg gtg gtc gac ctg ttc gaa agc tac        624
Asp Ala Ala Asp Glu Gly Phe Val Val Val Asp Leu Phe Glu Ser Tyr           
        195                 200                 205                       

ctg gac aag gcc gcc ggc gcg cac gcg gtg aac cca tga                    663
Leu Asp Lys Ala Ala Gly Ala His Ala Val Asn Pro                           
    210                 215                 220                           


<210>  10
<211>  220
<212>  PRT
<213>  Pseudomonas marginalis

<400>  10

Met Asp Gly Phe His Asp Leu Gly Gly Phe Gln Gly Phe Gly Lys Val 
1               5                   10                  15      


Pro His Thr Ile Asn Ser Leu Ser Tyr Lys Gln Val Phe Lys Gln Asp 
            20                  25                  30          


Trp Glu His Leu Ala Tyr Ser Leu Met Phe Ile Gly Ala Asp His Leu 
        35                  40                  45              


Lys Lys Phe Ser Val Asp Glu Val Arg His Ala Val Glu Arg Leu Asp 
    50                  55                  60                  


Val Arg Gln His Val Gly Thr Gln Tyr Tyr Glu Arg Tyr Val Ile Ala 
65                  70                  75                  80  


Thr Ala Thr Leu Leu Val Glu Thr Gly Val Ile Thr Gln Ala Glu Leu 
                85                  90                  95      


Asp Gln Ala Leu Gly Ser His Phe Lys Leu Ala Asn Pro Ala His Ala 
            100                 105                 110         


Glu Gly Arg Pro Ala Ile Thr Gly Arg Pro Pro Phe Glu Val Gly Asp 
        115                 120                 125             


Arg Val Val Val Arg Asp Glu Tyr Val Ala Gly His Ile Arg Met Pro 
    130                 135                 140                 


Ala Tyr Val Arg Gly Lys Glu Gly Val Val Leu His Arg Thr Ser Glu 
145                 150                 155                 160 


Lys Trp Pro Phe Pro Asp Ala Ile Gly His Gly Asp Val Ser Ala Ala 
                165                 170                 175     


His Gln Pro Thr Tyr His Val Glu Phe Ser Val Lys Asp Leu Trp Gly 
            180                 185                 190         


Asp Ala Ala Asp Glu Gly Phe Val Val Val Asp Leu Phe Glu Ser Tyr 
        195                 200                 205             


Leu Asp Lys Ala Ala Gly Ala His Ala Val Asn Pro 
    210                 215                 220 


<210>  11
<211>  639
<212>  DNA
<213>  Rhodococcus erythropolis


<220>
<221>  misc_feature
<223>  ReHNase beta-subunit_P13449.1

<220>
<221>  misc_feature
<223>  ReNHase beta-subunit_P13449.1

<220>
<221>  CDS
<222>  (1)..(639)

<400>  11
atg gat gga gta cac gat ctt gcc gga gta caa ggc ttc ggc aaa gtc         48
Met Asp Gly Val His Asp Leu Ala Gly Val Gln Gly Phe Gly Lys Val           
1               5                   10                  15                

ccg cat acc gtc aac gcc gac atc ggc ccc acc ttt cac gcc gaa tgg         96
Pro His Thr Val Asn Ala Asp Ile Gly Pro Thr Phe His Ala Glu Trp           
            20                  25                  30                    

gaa cac ctg ccc tac agc ctg atg ttc gcc ggt gtc gcc gaa ctc ggg        144
Glu His Leu Pro Tyr Ser Leu Met Phe Ala Gly Val Ala Glu Leu Gly           
        35                  40                  45                        

gcc ttc agc gtc gac gaa gtg cga tac gtc gtc gag cgg atg gag ccg        192
Ala Phe Ser Val Asp Glu Val Arg Tyr Val Val Glu Arg Met Glu Pro           
    50                  55                  60                            

cgc cac tac atg atg acc ccg tac tac gag agg tac gtc atc ggt gtc        240
Arg His Tyr Met Met Thr Pro Tyr Tyr Glu Arg Tyr Val Ile Gly Val           
65                  70                  75                  80            

gcg aca ttg atg gtc gaa aag gga atc ctg acg cag gac gaa ctc gaa        288
Ala Thr Leu Met Val Glu Lys Gly Ile Leu Thr Gln Asp Glu Leu Glu           
                85                  90                  95                

agc ctt gcg ggg gga ccg ttc cca ctg tca cgg ccc agc gaa tcc gaa        336
Ser Leu Ala Gly Gly Pro Phe Pro Leu Ser Arg Pro Ser Glu Ser Glu           
            100                 105                 110                   

ggg cgg ccg gca ccc gtc gag acg acc acc ttc gaa gtc ggg cag cga        384
Gly Arg Pro Ala Pro Val Glu Thr Thr Thr Phe Glu Val Gly Gln Arg           
        115                 120                 125                       

gta cgc gta cgc gac gag tac gtt ccg ggg cat att cga atg cct gca        432
Val Arg Val Arg Asp Glu Tyr Val Pro Gly His Ile Arg Met Pro Ala           
    130                 135                 140                           

tac tgc cgt gga cga gtg gga acc atc tct cat cga act acc gag aag        480
Tyr Cys Arg Gly Arg Val Gly Thr Ile Ser His Arg Thr Thr Glu Lys           
145                 150                 155                 160           

tgg ccg ttt ccc gac gca atc ggc cac ggg cgc aac gac gcc ggc gaa        528
Trp Pro Phe Pro Asp Ala Ile Gly His Gly Arg Asn Asp Ala Gly Glu           
                165                 170                 175               

gaa ccg acg tac cac gtg aag ttc gcc gcc gag gaa ttg ttc ggt agc        576
Glu Pro Thr Tyr His Val Lys Phe Ala Ala Glu Glu Leu Phe Gly Ser           
            180                 185                 190                   

gac acc gac ggt gga agc gtc gtt gtc gac ctc ttc gag ggt tac ctc        624
Asp Thr Asp Gly Gly Ser Val Val Val Asp Leu Phe Glu Gly Tyr Leu           
        195                 200                 205                       

gag cct gcg gcc tga                                                    639
Glu Pro Ala Ala                                                           
    210                                                                   


<210>  12
<211>  212
<212>  PRT
<213>  Rhodococcus erythropolis

<400>  12

Met Asp Gly Val His Asp Leu Ala Gly Val Gln Gly Phe Gly Lys Val 
1               5                   10                  15      


Pro His Thr Val Asn Ala Asp Ile Gly Pro Thr Phe His Ala Glu Trp 
            20                  25                  30          


Glu His Leu Pro Tyr Ser Leu Met Phe Ala Gly Val Ala Glu Leu Gly 
        35                  40                  45              


Ala Phe Ser Val Asp Glu Val Arg Tyr Val Val Glu Arg Met Glu Pro 
    50                  55                  60                  


Arg His Tyr Met Met Thr Pro Tyr Tyr Glu Arg Tyr Val Ile Gly Val 
65                  70                  75                  80  


Ala Thr Leu Met Val Glu Lys Gly Ile Leu Thr Gln Asp Glu Leu Glu 
                85                  90                  95      


Ser Leu Ala Gly Gly Pro Phe Pro Leu Ser Arg Pro Ser Glu Ser Glu 
            100                 105                 110         


Gly Arg Pro Ala Pro Val Glu Thr Thr Thr Phe Glu Val Gly Gln Arg 
        115                 120                 125             


Val Arg Val Arg Asp Glu Tyr Val Pro Gly His Ile Arg Met Pro Ala 
    130                 135                 140                 


Tyr Cys Arg Gly Arg Val Gly Thr Ile Ser His Arg Thr Thr Glu Lys 
145                 150                 155                 160 


Trp Pro Phe Pro Asp Ala Ile Gly His Gly Arg Asn Asp Ala Gly Glu 
                165                 170                 175     


Glu Pro Thr Tyr His Val Lys Phe Ala Ala Glu Glu Leu Phe Gly Ser 
            180                 185                 190         


Asp Thr Asp Gly Gly Ser Val Val Val Asp Leu Phe Glu Gly Tyr Leu 
        195                 200                 205             


Glu Pro Ala Ala 
    210         


<210>  13
<211>  119
<212>  PRT
<213>  Pseudomonas kilonensis_PkNHase beta-subunit_partial sequence


<220>
<221>  misc_feature
<222>  (92)..(92)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (107)..(107)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (117)..(117)
<223>  Xaa can be any naturally occurring amino acid

<400>  13

Met Asp Gly Phe His Asp Leu Gly Gly Phe Gln Gly Phe Gly Lys Val 
1               5                   10                  15      


Pro His Thr Ile Asn Ser Leu Ser Tyr Lys Gln Val Phe Lys Gln Asp 
            20                  25                  30          


Trp Glu His Leu Ala Tyr Ser Leu Met Phe Val Gly Val Asp Gln Leu 
        35                  40                  45              


Lys Lys Phe Ser Val Asp Glu Val Arg His Ala Val Glu Arg Leu Asp 
    50                  55                  60                  


Val Arg Gln His Val Gly Thr Gln Tyr Tyr Glu Arg Tyr Val Ile Ala 
65                  70                  75                  80  


Thr Ala Thr Leu Leu Val Glu Thr Gly Val Ile Xaa Gln Ala Glu Leu 
                85                  90                  95      


Asp Gln Ala Leu Gly Ser His Phe Lys Leu Xaa Lys Pro Cys Ser Cys 
            100                 105                 110         


Gln Arg Ser Pro Xaa Asp His 
        115                 


<210>  14
<211>  633
<212>  DNA
<213>  Comamonas testosteroni


<220>
<221>  misc_feature
<223>  CtNHase alpha-subunit_AAU87542.1

<220>
<221>  CDS
<222>  (1)..(633)

<400>  14
atg ggg caa tca cac acg cat gac cac cat cac gac ggg tac cag gca         48
Met Gly Gln Ser His Thr His Asp His His His Asp Gly Tyr Gln Ala           
1               5                   10                  15                

ccg ccc gaa gac atc gcg ctg cgg gtc aag gcc ttg gag tct ctg ctg         96
Pro Pro Glu Asp Ile Ala Leu Arg Val Lys Ala Leu Glu Ser Leu Leu           
            20                  25                  30                    

atc gag aaa ggt ctt gtc gac cca gcg gcc atg gac ttg gtc gtc caa        144
Ile Glu Lys Gly Leu Val Asp Pro Ala Ala Met Asp Leu Val Val Gln           
        35                  40                  45                        

acg tat gaa cac aag gta ggc ccc cga aac ggc gcc aaa gtc gtg gcc        192
Thr Tyr Glu His Lys Val Gly Pro Arg Asn Gly Ala Lys Val Val Ala           
    50                  55                  60                            

aag gcc tgg gtg gac cct gcc tac aag gcc cgt ctg ctg gca gac ggc        240
Lys Ala Trp Val Asp Pro Ala Tyr Lys Ala Arg Leu Leu Ala Asp Gly           
65                  70                  75                  80            

act gcc ggc att gcc gag ctg ggc ttc tcc ggg gta cag ggc gag gac        288
Thr Ala Gly Ile Ala Glu Leu Gly Phe Ser Gly Val Gln Gly Glu Asp           
                85                  90                  95                

atg gtc att ctg gaa aac acc ccc gcc gtc cac aac gtc gtc gtt tgc        336
Met Val Ile Leu Glu Asn Thr Pro Ala Val His Asn Val Val Val Cys           
            100                 105                 110                   

acc ttg tgc tct tgc tac cca tgg ccg acg ctg ggc ttg ccc cct gcc        384
Thr Leu Cys Ser Cys Tyr Pro Trp Pro Thr Leu Gly Leu Pro Pro Ala           
        115                 120                 125                       

tgg tac aag gcc ccg ccc tac cgg tcc cgc atg gtg agc gac ccg cgt        432
Trp Tyr Lys Ala Pro Pro Tyr Arg Ser Arg Met Val Ser Asp Pro Arg           
    130                 135                 140                           

ggg gtt ctc gcg gag ttc ggc ctg gtg atc ccc gcg aag gaa atc cgc        480
Gly Val Leu Ala Glu Phe Gly Leu Val Ile Pro Ala Lys Glu Ile Arg           
145                 150                 155                 160           

gtc tgg gac acc acg gcc gaa ttg cgc tac atg gtg ctg ccg gaa cgg        528
Val Trp Asp Thr Thr Ala Glu Leu Arg Tyr Met Val Leu Pro Glu Arg           
                165                 170                 175               

ccc gcg gga act gaa gcc tac agc gaa gaa caa ctg gcc gaa ctc gtt        576
Pro Ala Gly Thr Glu Ala Tyr Ser Glu Glu Gln Leu Ala Glu Leu Val           
            180                 185                 190                   

acc cgc gat tcg atg atc ggc acc ggc ctg ccc atc caa ccc acc cca        624
Thr Arg Asp Ser Met Ile Gly Thr Gly Leu Pro Ile Gln Pro Thr Pro           
        195                 200                 205                       

tct cat taa                                                            633
Ser His                                                                   
    210                                                                   


<210>  15
<211>  210
<212>  PRT
<213>  Comamonas testosteroni

<400>  15

Met Gly Gln Ser His Thr His Asp His His His Asp Gly Tyr Gln Ala 
1               5                   10                  15      


Pro Pro Glu Asp Ile Ala Leu Arg Val Lys Ala Leu Glu Ser Leu Leu 
            20                  25                  30          


Ile Glu Lys Gly Leu Val Asp Pro Ala Ala Met Asp Leu Val Val Gln 
        35                  40                  45              


Thr Tyr Glu His Lys Val Gly Pro Arg Asn Gly Ala Lys Val Val Ala 
    50                  55                  60                  


Lys Ala Trp Val Asp Pro Ala Tyr Lys Ala Arg Leu Leu Ala Asp Gly 
65                  70                  75                  80  


Thr Ala Gly Ile Ala Glu Leu Gly Phe Ser Gly Val Gln Gly Glu Asp 
                85                  90                  95      


Met Val Ile Leu Glu Asn Thr Pro Ala Val His Asn Val Val Val Cys 
            100                 105                 110         


Thr Leu Cys Ser Cys Tyr Pro Trp Pro Thr Leu Gly Leu Pro Pro Ala 
        115                 120                 125             


Trp Tyr Lys Ala Pro Pro Tyr Arg Ser Arg Met Val Ser Asp Pro Arg 
    130                 135                 140                 


Gly Val Leu Ala Glu Phe Gly Leu Val Ile Pro Ala Lys Glu Ile Arg 
145                 150                 155                 160 


Val Trp Asp Thr Thr Ala Glu Leu Arg Tyr Met Val Leu Pro Glu Arg 
                165                 170                 175     


Pro Ala Gly Thr Glu Ala Tyr Ser Glu Glu Gln Leu Ala Glu Leu Val 
            180                 185                 190         


Thr Arg Asp Ser Met Ile Gly Thr Gly Leu Pro Ile Gln Pro Thr Pro 
        195                 200                 205             


Ser His 
    210 


<210>  16
<211>  609
<212>  DNA
<213>  Klebsiella oxytoca


<220>
<221>  misc_feature
<223>  KoNHase alpha-subunit_OSY94202.1

<220>
<221>  CDS
<222>  (1)..(609)

<400>  16
atg agc cat aaa cac gac cac gac cat acc cac ccc ccc gtt gat atc         48
Met Ser His Lys His Asp His Asp His Thr His Pro Pro Val Asp Ile           
1               5                   10                  15                

gag cta cgc gtc cgc gca ctg gaa tcc ctg ctg cag gaa aaa ggc ctg         96
Glu Leu Arg Val Arg Ala Leu Glu Ser Leu Leu Gln Glu Lys Gly Leu           
            20                  25                  30                    

atc gac ccg gct gcg ctg gat gag ctg att gac acc tac gag cac aaa        144
Ile Asp Pro Ala Ala Leu Asp Glu Leu Ile Asp Thr Tyr Glu His Lys           
        35                  40                  45                        

gtc ggc ccc cga aac ggc gca cag gtt gtc gcc aga gcg tgg agc gac        192
Val Gly Pro Arg Asn Gly Ala Gln Val Val Ala Arg Ala Trp Ser Asp           
    50                  55                  60                            

ccg gaa tac aaa cgt cga ctg atg gaa aac gcc act gcc gct att gct        240
Pro Glu Tyr Lys Arg Arg Leu Met Glu Asn Ala Thr Ala Ala Ile Ala           
65                  70                  75                  80            

gaa ctg ggt ttc tcc gga ata cag ggc gaa gac atg ctg gtc gtg gag        288
Glu Leu Gly Phe Ser Gly Ile Gln Gly Glu Asp Met Leu Val Val Glu           
                85                  90                  95                

aac acg ccg gac gtg cat aac gtc acc gtt tgt acg ctg tgt tcc tgc        336
Asn Thr Pro Asp Val His Asn Val Thr Val Cys Thr Leu Cys Ser Cys           
            100                 105                 110                   

tac ccc tgg ccg gtg ctg ggt ctg ccg ccg gtg tgg tac aaa tca gcg        384
Tyr Pro Trp Pro Val Leu Gly Leu Pro Pro Val Trp Tyr Lys Ser Ala           
        115                 120                 125                       

ccc tat cgt tcg cgt atc gtc atc gac ccg cgc ggc gtt ctc gcc gag        432
Pro Tyr Arg Ser Arg Ile Val Ile Asp Pro Arg Gly Val Leu Ala Glu           
    130                 135                 140                           

ttc ggg tta cac ata cca gaa aac aaa gag att cgc gtc tgg gac agc        480
Phe Gly Leu His Ile Pro Glu Asn Lys Glu Ile Arg Val Trp Asp Ser           
145                 150                 155                 160           

agc gcc gag ctg cgc tat ctg gtc ctg cct gaa cgt ccg gca ggc acg        528
Ser Ala Glu Leu Arg Tyr Leu Val Leu Pro Glu Arg Pro Ala Gly Thr           
                165                 170                 175               

gaa ggc tgg agc gaa gcg cag ttg agc gaa ctc atc acg cgc gat tcg        576
Glu Gly Trp Ser Glu Ala Gln Leu Ser Glu Leu Ile Thr Arg Asp Ser           
            180                 185                 190                   

atg att ggc acc ggt gtg gtt agc gca cca taa                            609
Met Ile Gly Thr Gly Val Val Ser Ala Pro                                   
        195                 200                                           


<210>  17
<211>  202
<212>  PRT
<213>  Klebsiella oxytoca

<400>  17

Met Ser His Lys His Asp His Asp His Thr His Pro Pro Val Asp Ile 
1               5                   10                  15      


Glu Leu Arg Val Arg Ala Leu Glu Ser Leu Leu Gln Glu Lys Gly Leu 
            20                  25                  30          


Ile Asp Pro Ala Ala Leu Asp Glu Leu Ile Asp Thr Tyr Glu His Lys 
        35                  40                  45              


Val Gly Pro Arg Asn Gly Ala Gln Val Val Ala Arg Ala Trp Ser Asp 
    50                  55                  60                  


Pro Glu Tyr Lys Arg Arg Leu Met Glu Asn Ala Thr Ala Ala Ile Ala 
65                  70                  75                  80  


Glu Leu Gly Phe Ser Gly Ile Gln Gly Glu Asp Met Leu Val Val Glu 
                85                  90                  95      


Asn Thr Pro Asp Val His Asn Val Thr Val Cys Thr Leu Cys Ser Cys 
            100                 105                 110         


Tyr Pro Trp Pro Val Leu Gly Leu Pro Pro Val Trp Tyr Lys Ser Ala 
        115                 120                 125             


Pro Tyr Arg Ser Arg Ile Val Ile Asp Pro Arg Gly Val Leu Ala Glu 
    130                 135                 140                 


Phe Gly Leu His Ile Pro Glu Asn Lys Glu Ile Arg Val Trp Asp Ser 
145                 150                 155                 160 


Ser Ala Glu Leu Arg Tyr Leu Val Leu Pro Glu Arg Pro Ala Gly Thr 
                165                 170                 175     


Glu Gly Trp Ser Glu Ala Gln Leu Ser Glu Leu Ile Thr Arg Asp Ser 
            180                 185                 190         


Met Ile Gly Thr Gly Val Val Ser Ala Pro 
        195                 200         


<210>  18
<211>  636
<212>  DNA
<213>  Nitriliruptor alkaliphilus


<220>
<221>  misc_feature
<223>  NaNHase alpha-subunit_WP_052668589.1

<220>
<221>  CDS
<222>  (1)..(636)

<400>  18
atg agc cag acc cag agc cca ccc ccc gtc aac ttc agc ctg ccc cgc         48
Met Ser Gln Thr Gln Ser Pro Pro Pro Val Asn Phe Ser Leu Pro Arg           
1               5                   10                  15                

acg gag gag cag gtc gcg gcg cgc gtg aag gcg ctc gag tcg atc atg         96
Thr Glu Glu Gln Val Ala Ala Arg Val Lys Ala Leu Glu Ser Ile Met           
            20                  25                  30                    

atc gag aag ggc atc atg acg acg gac gcc gtc gac cgc ctc gcc gag        144
Ile Glu Lys Gly Ile Met Thr Thr Asp Ala Val Asp Arg Leu Ala Glu           
        35                  40                  45                        

atc tac gag aac gag gtc gga cca cag ctc ggc gca agg gtc gtg gcc        192
Ile Tyr Glu Asn Glu Val Gly Pro Gln Leu Gly Ala Arg Val Val Ala           
    50                  55                  60                            

cgc gcc tgg tct gac ccc gac ttc cac gat cgg ctg ctc gcc aac gcc        240
Arg Ala Trp Ser Asp Pro Asp Phe His Asp Arg Leu Leu Ala Asn Ala           
65                  70                  75                  80            

acc gag gcc tgc gag gag ctg gac atc ggc ggc ctg cag ggc gag gac        288
Thr Glu Ala Cys Glu Glu Leu Asp Ile Gly Gly Leu Gln Gly Glu Asp           
                85                  90                  95                

atg gtc gtc atc gag aac acc gac gac gtg cac aac ctc atc gtg tgc        336
Met Val Val Ile Glu Asn Thr Asp Asp Val His Asn Leu Ile Val Cys           
            100                 105                 110                   

acg ctc tgc tcc tgc tac ccc tgg cca acg ctc ggc ctg ccg ccc aac        384
Thr Leu Cys Ser Cys Tyr Pro Trp Pro Thr Leu Gly Leu Pro Pro Asn           
        115                 120                 125                       

tgg tac aag tac ccc gcc tac cgg gcc aag gcc gtg cgc gaa ccg cgg        432
Trp Tyr Lys Tyr Pro Ala Tyr Arg Ala Lys Ala Val Arg Glu Pro Arg           
    130                 135                 140                           

gcg atg ctg cgc gac gac ttc ggc ctc gac ctg ccc gac tcg gtt gag        480
Ala Met Leu Arg Asp Asp Phe Gly Leu Asp Leu Pro Asp Ser Val Glu           
145                 150                 155                 160           

atc cgc gtc tgg gat acc agc gcc gag ctt cgg tac tgg gtc ctg ccc        528
Ile Arg Val Trp Asp Thr Ser Ala Glu Leu Arg Tyr Trp Val Leu Pro           
                165                 170                 175               

aag cgc ccg gaa ggt acc gag agc atg acg gag gac gaa ttg gcc gag        576
Lys Arg Pro Glu Gly Thr Glu Ser Met Thr Glu Asp Glu Leu Ala Glu           
            180                 185                 190                   

ctg gtc acc cgc gac tcc atg atc ggt gtc ggc gtc gcc cgt acc ccc        624
Leu Val Thr Arg Asp Ser Met Ile Gly Val Gly Val Ala Arg Thr Pro           
        195                 200                 205                       

gag gcg gtc tga                                                        636
Glu Ala Val                                                               
    210                                                                   


<210>  19
<211>  211
<212>  PRT
<213>  Nitriliruptor alkaliphilus

<400>  19

Met Ser Gln Thr Gln Ser Pro Pro Pro Val Asn Phe Ser Leu Pro Arg 
1               5                   10                  15      


Thr Glu Glu Gln Val Ala Ala Arg Val Lys Ala Leu Glu Ser Ile Met 
            20                  25                  30          


Ile Glu Lys Gly Ile Met Thr Thr Asp Ala Val Asp Arg Leu Ala Glu 
        35                  40                  45              


Ile Tyr Glu Asn Glu Val Gly Pro Gln Leu Gly Ala Arg Val Val Ala 
    50                  55                  60                  


Arg Ala Trp Ser Asp Pro Asp Phe His Asp Arg Leu Leu Ala Asn Ala 
65                  70                  75                  80  


Thr Glu Ala Cys Glu Glu Leu Asp Ile Gly Gly Leu Gln Gly Glu Asp 
                85                  90                  95      


Met Val Val Ile Glu Asn Thr Asp Asp Val His Asn Leu Ile Val Cys 
            100                 105                 110         


Thr Leu Cys Ser Cys Tyr Pro Trp Pro Thr Leu Gly Leu Pro Pro Asn 
        115                 120                 125             


Trp Tyr Lys Tyr Pro Ala Tyr Arg Ala Lys Ala Val Arg Glu Pro Arg 
    130                 135                 140                 


Ala Met Leu Arg Asp Asp Phe Gly Leu Asp Leu Pro Asp Ser Val Glu 
145                 150                 155                 160 


Ile Arg Val Trp Asp Thr Ser Ala Glu Leu Arg Tyr Trp Val Leu Pro 
                165                 170                 175     


Lys Arg Pro Glu Gly Thr Glu Ser Met Thr Glu Asp Glu Leu Ala Glu 
            180                 185                 190         


Leu Val Thr Arg Asp Ser Met Ile Gly Val Gly Val Ala Arg Thr Pro 
        195                 200                 205             


Glu Ala Val 
    210     


<210>  20
<211>  624
<212>  DNA
<213>  Gordonia hydrophobica


<220>
<221>  misc_feature
<223>  GhNHase alpha-subunit_WP_066163464.1

<220>
<221>  CDS
<222>  (1)..(624)

<400>  20
atg tct gtc acc atc gat cac gca gtc gac aac gat ctc ggc gag aag         48
Met Ser Val Thr Ile Asp His Ala Val Asp Asn Asp Leu Gly Glu Lys           
1               5                   10                  15                

gct ccg cgc tcg gcg cgt ggc gaa gcg ctc cgt cgt gct ctg gag gcc         96
Ala Pro Arg Ser Ala Arg Gly Glu Ala Leu Arg Arg Ala Leu Glu Ala           
            20                  25                  30                    

aag ggc ctg ctg ccc gag ggc tat ctc gac gag tgg aac aac acc gca        144
Lys Gly Leu Leu Pro Glu Gly Tyr Leu Asp Glu Trp Asn Asn Thr Ala           
        35                  40                  45                        

gag aac gtc ttc agt ccg cgg cgc ggc gcc gaa ctc gtc gca cac gca        192
Glu Asn Val Phe Ser Pro Arg Arg Gly Ala Glu Leu Val Ala His Ala           
    50                  55                  60                            

tgg acc gac ccg gaa ttc cgc gag ctc ttg ctc act gac ggg acg gct        240
Trp Thr Asp Pro Glu Phe Arg Glu Leu Leu Leu Thr Asp Gly Thr Ala           
65                  70                  75                  80            

gcg gtg ggc cag tac ggt tac ctc ggc ccg cag ggc gag tac atc gtc        288
Ala Val Gly Gln Tyr Gly Tyr Leu Gly Pro Gln Gly Glu Tyr Ile Val           
                85                  90                  95                

gcg ttg gag gac acg ccg acg ctc aag cac gtc atc gtg tgc tcg ctc        336
Ala Leu Glu Asp Thr Pro Thr Leu Lys His Val Ile Val Cys Ser Leu           
            100                 105                 110                   

tgc tcg tgc acg gcc tgg ccg att ctg gga ctc tcg ccg tcg tgg tac        384
Cys Ser Cys Thr Ala Trp Pro Ile Leu Gly Leu Ser Pro Ser Trp Tyr           
        115                 120                 125                       

aag agc ttc gag tac cgg tcc cgg gtg gtg cgt gaa ccg cgc acg gtg        432
Lys Ser Phe Glu Tyr Arg Ser Arg Val Val Arg Glu Pro Arg Thr Val           
    130                 135                 140                           

ctc gcc gag atg ggc acc cag att ccg gac ggc gtc gag atc cgg gtc        480
Leu Ala Glu Met Gly Thr Gln Ile Pro Asp Gly Val Glu Ile Arg Val           
145                 150                 155                 160           

gtc gat gtc acc gcg gac acc cgc ttc atg gtt ctc ccg cag cgg ccg        528
Val Asp Val Thr Ala Asp Thr Arg Phe Met Val Leu Pro Gln Arg Pro           
                165                 170                 175               

gcg ggc aca gag ggg tgg agc cgt gaa cag ctg gcc gag atc gtg acc        576
Ala Gly Thr Glu Gly Trp Ser Arg Glu Gln Leu Ala Glu Ile Val Thr           
            180                 185                 190                   

aag gac tgc ttg atc ggc gtg gcg gtc cca cag gtc gaa tcc gtc tga        624
Lys Asp Cys Leu Ile Gly Val Ala Val Pro Gln Val Glu Ser Val               
        195                 200                 205                       


<210>  21
<211>  207
<212>  PRT
<213>  Gordonia hydrophobica

<400>  21

Met Ser Val Thr Ile Asp His Ala Val Asp Asn Asp Leu Gly Glu Lys 
1               5                   10                  15      


Ala Pro Arg Ser Ala Arg Gly Glu Ala Leu Arg Arg Ala Leu Glu Ala 
            20                  25                  30          


Lys Gly Leu Leu Pro Glu Gly Tyr Leu Asp Glu Trp Asn Asn Thr Ala 
        35                  40                  45              


Glu Asn Val Phe Ser Pro Arg Arg Gly Ala Glu Leu Val Ala His Ala 
    50                  55                  60                  


Trp Thr Asp Pro Glu Phe Arg Glu Leu Leu Leu Thr Asp Gly Thr Ala 
65                  70                  75                  80  


Ala Val Gly Gln Tyr Gly Tyr Leu Gly Pro Gln Gly Glu Tyr Ile Val 
                85                  90                  95      


Ala Leu Glu Asp Thr Pro Thr Leu Lys His Val Ile Val Cys Ser Leu 
            100                 105                 110         


Cys Ser Cys Thr Ala Trp Pro Ile Leu Gly Leu Ser Pro Ser Trp Tyr 
        115                 120                 125             


Lys Ser Phe Glu Tyr Arg Ser Arg Val Val Arg Glu Pro Arg Thr Val 
    130                 135                 140                 


Leu Ala Glu Met Gly Thr Gln Ile Pro Asp Gly Val Glu Ile Arg Val 
145                 150                 155                 160 


Val Asp Val Thr Ala Asp Thr Arg Phe Met Val Leu Pro Gln Arg Pro 
                165                 170                 175     


Ala Gly Thr Glu Gly Trp Ser Arg Glu Gln Leu Ala Glu Ile Val Thr 
            180                 185                 190         


Lys Asp Cys Leu Ile Gly Val Ala Val Pro Gln Val Glu Ser Val 
        195                 200                 205         


<210>  22
<211>  585
<212>  DNA
<213>  Pseudomonas marginalis


<220>
<221>  misc_feature
<223>  PmNHase alpha-subunit_WP_074846646.1

<220>
<221>  CDS
<222>  (1)..(585)

<400>  22
atg aat aca gcg act tca acg ccc ggc gaa aga gcc tgg gca ttg ttt         48
Met Asn Thr Ala Thr Ser Thr Pro Gly Glu Arg Ala Trp Ala Leu Phe           
1               5                   10                  15                

caa gtc ctc aag agc aag gaa ctc atc ccg gag ggc tat gtc gag cag         96
Gln Val Leu Lys Ser Lys Glu Leu Ile Pro Glu Gly Tyr Val Glu Gln           
            20                  25                  30                    

ctc acg caa ttg atg gag cac ggc tgg agc ccc gag aac ggc gcc cgt        144
Leu Thr Gln Leu Met Glu His Gly Trp Ser Pro Glu Asn Gly Ala Arg           
        35                  40                  45                        

gtg gtg gcc aag gca tgg gtc gat ccg cag ttc cgg gca ctg ttg ctc        192
Val Val Ala Lys Ala Trp Val Asp Pro Gln Phe Arg Ala Leu Leu Leu           
    50                  55                  60                            

aag gac ggc acc gcg gcc tgc gcc cag ttc ggc tac acc ggc ccc cag        240
Lys Asp Gly Thr Ala Ala Cys Ala Gln Phe Gly Tyr Thr Gly Pro Gln           
65                  70                  75                  80            

ggc gaa tac atc gtc gcc ctg gag gat acg ccg acg ctg aag aac gtg        288
Gly Glu Tyr Ile Val Ala Leu Glu Asp Thr Pro Thr Leu Lys Asn Val           
                85                  90                  95                

atc gtc tgc agc ctg tgc tcc tgc acc aac tgg ccg gtc ctc ggc ctg        336
Ile Val Cys Ser Leu Cys Ser Cys Thr Asn Trp Pro Val Leu Gly Leu           
            100                 105                 110                   

ccg ccg gag tgg tac aag ggt ttc gag ttc cgc gca cgc ctg gtc cgg        384
Pro Pro Glu Trp Tyr Lys Gly Phe Glu Phe Arg Ala Arg Leu Val Arg           
        115                 120                 125                       

gag ggg cgc acg gta ctg cgc gag ctg ggg acg gag ttg ccc cgg gac        432
Glu Gly Arg Thr Val Leu Arg Glu Leu Gly Thr Glu Leu Pro Arg Asp           
    130                 135                 140                           

atg gtg gtc aag gtc tgg gat acc agc gcc gaa agc cgc tac ctg gtg        480
Met Val Val Lys Val Trp Asp Thr Ser Ala Glu Ser Arg Tyr Leu Val           
145                 150                 155                 160           

ctg ccg gtc agg ccg gaa ggc tca gaa cac atg agc gaa gag cag ctt        528
Leu Pro Val Arg Pro Glu Gly Ser Glu His Met Ser Glu Glu Gln Leu           
                165                 170                 175               

caa gcg ctg gtg acc aaa gac gtg ctg atc ggc gtc gcc ctg ccc cgc        576
Gln Ala Leu Val Thr Lys Asp Val Leu Ile Gly Val Ala Leu Pro Arg           
            180                 185                 190                   

gtg ggc tga                                                            585
Val Gly                                                                   
                                                                          


<210>  23
<211>  194
<212>  PRT
<213>  Pseudomonas marginalis

<400>  23

Met Asn Thr Ala Thr Ser Thr Pro Gly Glu Arg Ala Trp Ala Leu Phe 
1               5                   10                  15      


Gln Val Leu Lys Ser Lys Glu Leu Ile Pro Glu Gly Tyr Val Glu Gln 
            20                  25                  30          


Leu Thr Gln Leu Met Glu His Gly Trp Ser Pro Glu Asn Gly Ala Arg 
        35                  40                  45              


Val Val Ala Lys Ala Trp Val Asp Pro Gln Phe Arg Ala Leu Leu Leu 
    50                  55                  60                  


Lys Asp Gly Thr Ala Ala Cys Ala Gln Phe Gly Tyr Thr Gly Pro Gln 
65                  70                  75                  80  


Gly Glu Tyr Ile Val Ala Leu Glu Asp Thr Pro Thr Leu Lys Asn Val 
                85                  90                  95      


Ile Val Cys Ser Leu Cys Ser Cys Thr Asn Trp Pro Val Leu Gly Leu 
            100                 105                 110         


Pro Pro Glu Trp Tyr Lys Gly Phe Glu Phe Arg Ala Arg Leu Val Arg 
        115                 120                 125             


Glu Gly Arg Thr Val Leu Arg Glu Leu Gly Thr Glu Leu Pro Arg Asp 
    130                 135                 140                 


Met Val Val Lys Val Trp Asp Thr Ser Ala Glu Ser Arg Tyr Leu Val 
145                 150                 155                 160 


Leu Pro Val Arg Pro Glu Gly Ser Glu His Met Ser Glu Glu Gln Leu 
                165                 170                 175     


Gln Ala Leu Val Thr Lys Asp Val Leu Ile Gly Val Ala Leu Pro Arg 
            180                 185                 190         


Val Gly 
        


<210>  24
<211>  624
<212>  DNA
<213>  Rhodococcus erythropolis


<220>
<221>  misc_feature
<223>  ReNHase alpha-subunit_P13448.3

<220>
<221>  CDS
<222>  (1)..(624)

<400>  24
atg tca gta acg atc gac cac aca acg gag aac gcc gca ccg gcc cag         48
Met Ser Val Thr Ile Asp His Thr Thr Glu Asn Ala Ala Pro Ala Gln           
1               5                   10                  15                

gcg ccg gtc tcc gac cgg gcg tgg gca ctg ttc cgc gca ctc gac ggt         96
Ala Pro Val Ser Asp Arg Ala Trp Ala Leu Phe Arg Ala Leu Asp Gly           
            20                  25                  30                    

aag gga ttg gta ccc gac ggt tac gtc gag gga tgg aag aag acc ttc        144
Lys Gly Leu Val Pro Asp Gly Tyr Val Glu Gly Trp Lys Lys Thr Phe           
        35                  40                  45                        

gag gag gac ttc agt cca agg cgc gga gcg gaa ttg gta gcg cgc gca        192
Glu Glu Asp Phe Ser Pro Arg Arg Gly Ala Glu Leu Val Ala Arg Ala           
    50                  55                  60                            

tgg acc gac ccc gag ttc cgg cag ctg ctt ctc acc gac ggt acc gcc        240
Trp Thr Asp Pro Glu Phe Arg Gln Leu Leu Leu Thr Asp Gly Thr Ala           
65                  70                  75                  80            

gca gtt gcc cag tac gga tac ctg ggc ccc cag ggc gaa tac atc gtg        288
Ala Val Ala Gln Tyr Gly Tyr Leu Gly Pro Gln Gly Glu Tyr Ile Val           
                85                  90                  95                

gca gtc gaa gac acc ccg aca ctc aag aac gtg atc gtg tgc tcg ctg        336
Ala Val Glu Asp Thr Pro Thr Leu Lys Asn Val Ile Val Cys Ser Leu           
            100                 105                 110                   

tgt tca tgc acc gcg tgg ccc atc ctc ggt ctg cca ccc acc tgg tac        384
Cys Ser Cys Thr Ala Trp Pro Ile Leu Gly Leu Pro Pro Thr Trp Tyr           
        115                 120                 125                       

aag agc ttc gaa tac cgt gcg cgc gtg gtc cgc gaa cca cgg aag gtt        432
Lys Ser Phe Glu Tyr Arg Ala Arg Val Val Arg Glu Pro Arg Lys Val           
    130                 135                 140                           

ctc tcc gag atg gga acc gag atc gcg tcg gac atc gag att cgc gtc        480
Leu Ser Glu Met Gly Thr Glu Ile Ala Ser Asp Ile Glu Ile Arg Val           
145                 150                 155                 160           

tac gac acc acc gcc gaa act cgc tac atg gtc ctc ccg cag cgt ccc        528
Tyr Asp Thr Thr Ala Glu Thr Arg Tyr Met Val Leu Pro Gln Arg Pro           
                165                 170                 175               

gcc ggc acc gaa ggc tgg agc cag gaa caa ctg cag gaa atc gtc acc        576
Ala Gly Thr Glu Gly Trp Ser Gln Glu Gln Leu Gln Glu Ile Val Thr           
            180                 185                 190                   

aag gac tgc ctg atc ggg gtt gca atc ccg cag gtt ccc acc gtc tga        624
Lys Asp Cys Leu Ile Gly Val Ala Ile Pro Gln Val Pro Thr Val               
        195                 200                 205                       


<210>  25
<211>  207
<212>  PRT
<213>  Rhodococcus erythropolis

<400>  25

Met Ser Val Thr Ile Asp His Thr Thr Glu Asn Ala Ala Pro Ala Gln 
1               5                   10                  15      


Ala Pro Val Ser Asp Arg Ala Trp Ala Leu Phe Arg Ala Leu Asp Gly 
            20                  25                  30          


Lys Gly Leu Val Pro Asp Gly Tyr Val Glu Gly Trp Lys Lys Thr Phe 
        35                  40                  45              


Glu Glu Asp Phe Ser Pro Arg Arg Gly Ala Glu Leu Val Ala Arg Ala 
    50                  55                  60                  


Trp Thr Asp Pro Glu Phe Arg Gln Leu Leu Leu Thr Asp Gly Thr Ala 
65                  70                  75                  80  


Ala Val Ala Gln Tyr Gly Tyr Leu Gly Pro Gln Gly Glu Tyr Ile Val 
                85                  90                  95      


Ala Val Glu Asp Thr Pro Thr Leu Lys Asn Val Ile Val Cys Ser Leu 
            100                 105                 110         


Cys Ser Cys Thr Ala Trp Pro Ile Leu Gly Leu Pro Pro Thr Trp Tyr 
        115                 120                 125             


Lys Ser Phe Glu Tyr Arg Ala Arg Val Val Arg Glu Pro Arg Lys Val 
    130                 135                 140                 


Leu Ser Glu Met Gly Thr Glu Ile Ala Ser Asp Ile Glu Ile Arg Val 
145                 150                 155                 160 


Tyr Asp Thr Thr Ala Glu Thr Arg Tyr Met Val Leu Pro Gln Arg Pro 
                165                 170                 175     


Ala Gly Thr Glu Gly Trp Ser Gln Glu Gln Leu Gln Glu Ile Val Thr 
            180                 185                 190         


Lys Asp Cys Leu Ile Gly Val Ala Ile Pro Gln Val Pro Thr Val 
        195                 200                 205         


<210>  26
<211>  603
<212>  DNA
<213>  Pseudomonas kilonensis


<220>
<221>  misc_feature
<223>  PkNHase alpha-subunit

<220>
<221>  CDS
<222>  (1)..(603)

<400>  26
atg agt aca tct att tcc acg act gcg acg act tca acg ccc gga gag         48
Met Ser Thr Ser Ile Ser Thr Thr Ala Thr Thr Ser Thr Pro Gly Glu           
1               5                   10                  15                

cgg gca cgg gca ctg ttt cag gtg ctc aag agc aaa gac ctc atc ccg         96
Arg Ala Arg Ala Leu Phe Gln Val Leu Lys Ser Lys Asp Leu Ile Pro           
            20                  25                  30                    

cag ggc tat gtc gag caa ctg act gag ttg atg gaa cac ggc tgg agc        144
Gln Gly Tyr Val Glu Gln Leu Thr Glu Leu Met Glu His Gly Trp Ser           
        35                  40                  45                        

ccg gag aac ggc gcc cga gtg gtc gcc aaa gca tgg gtc gat ccg cag        192
Pro Glu Asn Gly Ala Arg Val Val Ala Lys Ala Trp Val Asp Pro Gln           
    50                  55                  60                            

ttc cgg gca ctg ttg ctc aag gac ggc acc gcc gcg tgc gcc cag ttc        240
Phe Arg Ala Leu Leu Leu Lys Asp Gly Thr Ala Ala Cys Ala Gln Phe           
65                  70                  75                  80            

ggc tac acc ggt ccg cag ggc gag tac atc gtc gct ctg gag gat acg        288
Gly Tyr Thr Gly Pro Gln Gly Glu Tyr Ile Val Ala Leu Glu Asp Thr           
                85                  90                  95                

ccc gag gtg aaa aac gtc att gtc tgc agc ctt tgc tcc tgc acc aac        336
Pro Glu Val Lys Asn Val Ile Val Cys Ser Leu Cys Ser Cys Thr Asn           
            100                 105                 110                   

tgg ccg gtc ctc ggc ctg cca ccc gag tgg tac aag ggc ttt gag ttt        384
Trp Pro Val Leu Gly Leu Pro Pro Glu Trp Tyr Lys Gly Phe Glu Phe           
        115                 120                 125                       

cgt gct cgc ctg gtc cgg gaa gga cgc acc gta ctg cgt gaa ctg ggg        432
Arg Ala Arg Leu Val Arg Glu Gly Arg Thr Val Leu Arg Glu Leu Gly           
    130                 135                 140                           

aca gag tta ccg aac gac agg gtt gtc aag gtc tgg gac acc agc gcc        480
Thr Glu Leu Pro Asn Asp Arg Val Val Lys Val Trp Asp Thr Ser Ala           
145                 150                 155                 160           

gaa acc cgc tac ctg gtg ttg ccg gta aga cca gaa ggc tgc gag cac        528
Glu Thr Arg Tyr Leu Val Leu Pro Val Arg Pro Glu Gly Cys Glu His           
                165                 170                 175               

atg acc gaa gag cag ctt cgg aca ctg gtg acc aag gac gtg ctg att        576
Met Thr Glu Glu Gln Leu Arg Thr Leu Val Thr Lys Asp Val Leu Ile           
            180                 185                 190                   

ggc gtc gcc ctg ccc cag gtt ggc tga                                    603
Gly Val Ala Leu Pro Gln Val Gly                                           
        195                 200                                           


<210>  27
<211>  200
<212>  PRT
<213>  Pseudomonas kilonensis

<400>  27

Met Ser Thr Ser Ile Ser Thr Thr Ala Thr Thr Ser Thr Pro Gly Glu 
1               5                   10                  15      


Arg Ala Arg Ala Leu Phe Gln Val Leu Lys Ser Lys Asp Leu Ile Pro 
            20                  25                  30          


Gln Gly Tyr Val Glu Gln Leu Thr Glu Leu Met Glu His Gly Trp Ser 
        35                  40                  45              


Pro Glu Asn Gly Ala Arg Val Val Ala Lys Ala Trp Val Asp Pro Gln 
    50                  55                  60                  


Phe Arg Ala Leu Leu Leu Lys Asp Gly Thr Ala Ala Cys Ala Gln Phe 
65                  70                  75                  80  


Gly Tyr Thr Gly Pro Gln Gly Glu Tyr Ile Val Ala Leu Glu Asp Thr 
                85                  90                  95      


Pro Glu Val Lys Asn Val Ile Val Cys Ser Leu Cys Ser Cys Thr Asn 
            100                 105                 110         


Trp Pro Val Leu Gly Leu Pro Pro Glu Trp Tyr Lys Gly Phe Glu Phe 
        115                 120                 125             


Arg Ala Arg Leu Val Arg Glu Gly Arg Thr Val Leu Arg Glu Leu Gly 
    130                 135                 140                 


Thr Glu Leu Pro Asn Asp Arg Val Val Lys Val Trp Asp Thr Ser Ala 
145                 150                 155                 160 


Glu Thr Arg Tyr Leu Val Leu Pro Val Arg Pro Glu Gly Cys Glu His 
                165                 170                 175     


Met Thr Glu Glu Gln Leu Arg Thr Leu Val Thr Lys Asp Val Leu Ile 
            180                 185                 190         


Gly Val Ala Leu Pro Gln Val Gly 
        195                 200 


<210>  28
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Ct-aQ93X_for


<220>
<221>  misc_feature
<222>  (6)..(8)
<223>  N can be any nucleic acid (A, T, G, or C); K can be G or T

<400>  28
gggtannkgg cgaggacatg                                                   20


<210>  29
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Ct-aQ93X_rev


<220>
<221>  misc_feature
<222>  (4)..(6)
<223>  N can be any nucleic acid (A, T, G, or C); M can be A or C

<400>  29
gccmnntacc ccggagaag                                                    19


<210>  30
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Ct-aW120X_for


<220>
<221>  misc_feature
<222>  (7)..(9)
<223>  N can be any nucleic acid (A, T, G, or C); K can be G or T

<400>  30
tacccannkc cgacgctgg                                                    19


<210>  31
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Ct-aW120X_rev


<220>
<221>  misc_feature
<222>  (10)..(12)
<223>  N can be any nucleic acid (A, T, G, or C); M can be A or C

<400>  31
cagcgtcggm nntgggtag                                                    19


<210>  32
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Ct-aP126X_for


<220>
<221>  misc_feature
<222>  (9)..(11)
<223>  N can be any nucleic acid (A, T, G, or C); K can be G or T

<400>  32
tgggcttgnn kcctgcctg                                                    19


<210>  33
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Ct-aP126X_rev


<220>
<221>  misc_feature
<222>  (14)..(16)
<223>  N can be any nucleic acid (A, T, G, or C); M can be A or C

<400>  33
gtaccaggca ggmnncaag                                                    19


<210>  34
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Ct-aK131X_for


<220>
<221>  misc_feature
<222>  (5)..(7)
<223>  N can be any nucleic acid (A, T, G, or C); K can be G or T

<400>  34
gtacnnkgcc ccgccctac                                                    19


<210>  35
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Ct-aK131X_rev


<220>
<221>  misc_feature
<222>  (5)..(7)
<223>  N can be any nucleic acid (A, T, G, or C); M can be A or C

<400>  35
gggcmnngta ccaggcag                                                     18


<210>  36
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Ct-aR169X_for


<220>
<221>  misc_feature
<222>  (8)..(10)
<223>  N can be any nucleic acid (A, T, G, or C); K can be G or T

<400>  36
cgaattgnnk tacatggtgc tg                                                22


<210>  37
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Ct-aR169X_rev


<220>
<221>  misc_feature
<222>  (14)..(16)
<223>  N can be any nucleic acid (A, T, G, or C); M can be A or C

<400>  37
cagcaccatg tamnncaatt cg                                                22


<210>  38
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Ct-bM34X_for


<220>
<221>  misc_feature
<222>  (6)..(8)
<223>  N can be any nucleic acid (A, T, G, or C); K can be G or T

<400>  38
cggtcnnktc cctgttccc                                                    19


<210>  39
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Ct-bM34X_rev


<220>
<221>  misc_feature
<222>  (7)..(9)
<223>  N can be any nucleic acid (A, T, G, or C); M can be A or C

<400>  39
cagggamnng accgtttttt c                                                 21


<210>  40
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Ct-bF37X_for


<220>
<221>  misc_feature
<222>  (6)..(8)
<223>  N can be any nucleic acid (A, T, G, or C); K can be G or T

<400>  40
ccctgnnkcc ggcgctgttc                                                   20


<210>  41
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Ct-bF37X_rev


<220>
<221>  misc_feature
<222>  (6)..(8)
<223>  N can be any nucleic acid (A, T, G, or C); M can be A or C

<400>  41
gccggmnnca gggacatgac                                                   20


<210>  42
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Ct-bL48X_for


<220>
<221>  misc_feature
<222>  (5)..(7)
<223>  N can be any nucleic acid (A, T, G, or C); K can be G or T

<400>  42
caacnnkgat gagtttcgac ac                                                22


<210>  43
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Ct-bL48X_rev


<220>
<221>  misc_feature
<222>  (15)..(17)
<223>  N can be any nucleic acid (A, T, G, or C); M can be A or C

<400>  43
gtcgaaactc atcmnngttg aag                                               23


<210>  44
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Ct-bF51X_for


<220>
<221>  misc_feature
<222>  (8)..(10)
<223>  N can be any nucleic acid (A, T, G, or C); K can be G or T

<400>  44
cgatgagnnk cgacacggc                                                    19


<210>  45
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Ct-bF51X_rev


<220>
<221>  misc_feature
<222>  (10)..(12)
<223>  N can be any nucleic acid (A, T, G, or C); M can be A or C

<400>  45
gccgtgtcgm nnctcatcg                                                    19


<210>  46
<211>  32
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Ct-bY68X-long_for


<220>
<221>  misc_feature
<222>  (10)..(12)
<223>  N can be any nucleic acid (A, T, G, or C); K can be G or T

<400>  46
aagggaaccn nktacgaaca ctggatccat tc                                     32


<210>  47
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Ct-bY68X-long_rev


<220>
<221>  misc_feature
<222>  (9)..(11)
<223>  N can be any nucleic acid (A, T, G, or C); M can be A or C

<400>  47
tgttcgtamn nggttccctt caggtagtcg                                        30


<210>  48
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>   Primer Ct-aW120F_for

<400>  48
gctacccatt tccgacgctg g                                                 21


<210>  49
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer Ct-aW120F_rev

<400>  49
gcgtcggaaa tgggtagcaa gag                                               23


<210>  50
<211>  32
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer Ct-bM34L_for

<400>  50
aaacggtctt gtccctgttc ccggcgctgt tc                                     32


<210>  51
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer Ct-bM34L_rev

<400>  51
aacagggaca agaccgtttt ttcccagtcg tag                                    33


<210>  52
<211>  32
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer Ct-bM34Q_for

<400>  52
aaacggtcca gtccctgttc ccggcgctgt tc                                     32


<210>  53
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer Ct-bM34Q_rev

<400>  53
aacagggact ggaccgtttt ttcccagtcg tag                                    33


<210>  54
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Ct-alpha1_for

<400>  54
tggccaaggc ctgggtggac                                                   20


<210>  55
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Ct-alpha1_rev

<400>  55
gcaagagcac aaggtgcaaa c                                                 21


<210>  56
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Ct-alpha2_for

<400>  56
ccttgtgctc ttgctaccca                                                   20


<210>  57
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Ct-alpha2_rev

<400>  57
ttcagttccc gcgggccg                                                     18


<210>  58
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Ct-beta1_for

<400>  58
cgtctttcgc tacgactgg                                                    19


<210>  59
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Ct-beta1_rev

<400>  59
aggtttcgat ggaatggatc ca                                                22


<210>  60
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Ct-beta2_for

<400>  60
gcttctgccg cccgggag                                                     18


<210>  61
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Ct-beta2_rev

<400>  61
tttccgtgtg ccgcggtgtc                                                   20


<210>  62
<211>  34
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Ct-A1bb-lig_for


<220>
<221>  misc_feature
<222>  (6)..(11)
<223>  restriction site

<400>  62
aatttctcga gtttgcacct tgtgctcttg ctac                                   34


<210>  63
<211>  29
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Ct-A1bb-lig_rev


<220>
<221>  misc_feature
<222>  (6)..(11)
<223>  restriction site

<400>  63
aatttctcga gtccacccag gccttggcc                                         29


<210>  64
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Ct-A2bb-lig_for


<220>
<221>  misc_feature
<222>  (6)..(11)
<223>  restriction site

<400>  64
aatttctcga ggcccgcggg aactgaag                                          28


<210>  65
<211>  29
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Ct-A2bb-lig_rev


<220>
<221>  misc_feature
<222>  (6)..(11)
<223>  restriction site

<400>  65
aatttctcga gggtagcaag agcacaagg                                         29


<210>  66
<211>  36
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Ct-B1bb-lig_for


<220>
<221>  misc_feature
<222>  (7)..(12)
<223>  restriction site

<400>  66
tttaaagcta gctggatcca ttccatcgaa accttg                                 36


<210>  67
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Ct-B1bb-lig_rev


<220>
<221>  misc_feature
<222>  (7)..(12)
<223>  restriction site

<400>  67
tttaaagcta gccagtcgta gcgaaagacg                                        30


<210>  68
<211>  31
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Ct-B2bb-lig_for


<220>
<221>  misc_feature
<222>  (7)..(12)
<223>  restriction site

<400>  68
tttaaagcta gcaccgcggc acacggaaag g                                      31


<210>  69
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Ct-B2bb-lig_rev


<220>
<221>  misc_feature
<222>  (7)..(12)
<223>  restriction site

<400>  69
tttaaagcta gctcccgggc ggcagaagcc                                        30


<210>  70
<211>  44
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Ct-beta1-focused_for


<220>
<221>  misc_feature
<222>  (9)..(9)
<223>  Y can be T or C

<220>
<221>  misc_feature
<222>  (10)..(10)
<223>  K can be G or T

<220>
<221>  misc_feature
<222>  (11)..(11)
<223>  S can be C or G

<220>
<221>  misc_feature
<222>  (19)..(19)
<223>  V can be A, G or C

<220>
<221>  misc_feature
<222>  (27)..(27)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (29)..(29)
<223>  N can be A, G, T or C

<220>
<221>  misc_feature
<222>  (30)..(30)
<223>  D can be A, G or T

<400>  70
acttcaacyk sgatgagvtt cgacacndta tcgagcgcat gaac                        44


<210>  71
<211>  50
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Ct-beta1-focused_rev


<220>
<221>  misc_feature
<222>  (15)..(15)
<223>  H can be A, C or T

<220>
<221>  misc_feature
<222>  (16)..(16)
<223>  N can be A, G, T or C

<220>
<221>  misc_feature
<222>  (26)..(26)
<223>  B can be G, C or T

<220>
<221>  misc_feature
<222>  (34)..(34)
<223>  S can be C or G

<220>
<221>  misc_feature
<222>  (35)..(35)
<223>  M can be A or C

<220>
<221>  misc_feature
<222>  (36)..(36)
<223>  R can be G or A

<400>  71
catgcgctcg atahngtgtc gaabctcatc smrgttgaag ttgccgttgg                  50


<210>  72
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer Ct-P121A_for

<400>  72
atgggcgacg ctgggcttgc                                                   20


<210>  73
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer Ct-P121A_rev

<400>  73
agcgtcgccc atgggtagca agag                                              24


<210>  74
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer Ct-P121R_for

<400>  74
atggcgtacg ctgggcttgc                                                   20


<210>  75
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer Ct-P121R_rev

<400>  75
agcgtacgcc atgggtagca agag                                              24


<210>  76
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer Ct-P121N_for

<400>  76
atggaacacg ctgggcttgc                                                   20


<210>  77
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer Ct-P121N_rev

<400>  77
agcgtgttcc atgggtagca agag                                              24


<210>  78
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer Ct-P121D_for

<400>  78
atgggatacg ctgggcttgc                                                   20


<210>  79
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer Ct-P121D_rev

<400>  79
agcgtatccc atgggtagca agag                                              24


<210>  80
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer Ct-P121C_for

<400>  80
atggtgcacg ctgggcttgc                                                   20


<210>  81
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer Ct-P121C_rev

<400>  81
agcgtgcacc atgggtagca agag                                              24


<210>  82
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer Ct-P121Q_for

<400>  82
atggcagacg ctgggcttgc                                                   20


<210>  83
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer Ct-P121Q_rev

<400>  83
agcgtctgcc atgggtagca agag                                              24


<210>  84
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer Ct-P121E_for

<400>  84
atgggaaacg ctgggcttgc                                                   20


<210>  85
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer Ct-P121E_rev

<400>  85
agcgtttccc atgggtagca agag                                              24


<210>  86
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer Ct-P121G_for

<400>  86
atggggcacg ctgggcttgc                                                   20


<210>  87
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer Ct-P121G_rev

<400>  87
agcgtgcccc atgggtagca agag                                              24


<210>  88
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer Ct-P121H_for

<400>  88
atggcatacg ctgggcttgc                                                   20


<210>  89
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer Ct-P121H_rev

<400>  89
agcgtatgcc atgggtagca agag                                              24


<210>  90
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer Ct-P121I_for

<400>  90
atggattacg ctgggcttgc                                                   20


<210>  91
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer Ct-P121I_rev

<400>  91
agcgtaatcc atgggtagca agag                                              24


<210>  92
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer Ct-P121L_for

<400>  92
atggctgacg ctgggcttgc                                                   20


<210>  93
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer Ct-P121L_rev

<400>  93
agcgtcagcc atgggtagca agag                                              24


<210>  94
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer Ct-P121K_for

<400>  94
atggaaaacg ctgggcttgc                                                   20


<210>  95
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer Ct-P121K_rev

<400>  95
agcgttttcc atgggtagca agag                                              24


<210>  96
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer Ct-P121M_for

<400>  96
atggatgacg ctgggcttgc                                                   20


<210>  97
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer Ct-P121M_rev

<400>  97
agcgtcatcc atgggtagca agag                                              24


<210>  98
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer Ct-P121F_for

<400>  98
atggtttacg ctgggcttgc                                                   20


<210>  99
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer Ct-P121F_rev

<400>  99
agcgtaaacc atgggtagca agag                                              24


<210>  100
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer Ct-P121W_for

<400>  100
atggtggacg ctgggcttgc                                                   20


<210>  101
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer Ct-P121W_rev

<400>  101
agcgtccacc atgggtagca agag                                              24


<210>  102
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer Ct-P121Y_for

<400>  102
atggtatacg ctgggcttgc                                                   20


<210>  103
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer Ct-P121Y_rev

<400>  103
agcgtatacc atgggtagca agag                                              24


<210>  104
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Ct-P121T_for

<400>  104
atggacgacg ctgggcttgc                                                   20


<210>  105
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Ct-P121T_rev

<400>  105
agcgtcgtcc atgggtagca agag                                              24


<210>  106
<211>  29
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Ct-V110I_for

<400>  106
aacgtcatcg tttgcacctt gtgctcttg                                         29


<210>  107
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Ct-V110I_rev

<400>  107
aaacgatgac gttgtggacg gc                                                22


<210>  108
<211>  44
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Ct-L48R-G54C_for

<400>  108
acttcaaccg tgatgagttt cgacactgta tcgagcgcat gaac                        44


<210>  109
<211>  50
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Ct-L48R-G54C_rev

<400>  109
catgcgctcg atacagtgtc gaaactcatc acggttgaag ttgccgttgg                  50


<210>  110
<211>  44
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Ct-L48R-G54R_for

<400>  110
acttcaaccg tgatgagttt cgacaccgta tcgagcgcat gaac                        44


<210>  111
<211>  50
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Ct-L48R-G54R_rev

<400>  111
catgcgctcg atacggtgtc gaaactcatc acggttgaag ttgccgttgg                  50


<210>  112
<211>  44
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Ct-L48R-G54V_for

<400>  112
acttcaaccg tgatgagttt cgacacgtta tcgagcgcat gaac                        44


<210>  113
<211>  50
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Ct-L48R-G54V_rev

<400>  113
catgcgctcg ataacgtgtc gaaactcatc acggttgaag ttgccgttgg                  50


<210>  114
<211>  44
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Ct-L48P-G54C_for

<400>  114
acttcaaccc tgatgagttt cgacactgta tcgagcgcat gaac                        44


<210>  115
<211>  50
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Ct-L48P-G54C_rev

<400>  115
catgcgctcg atacagtgtc gaaactcatc agggttgaag ttgccgttgg                  50


<210>  116
<211>  44
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Ct-L48P-G54R_for

<400>  116
acttcaaccc tgatgagttt cgacaccgta tcgagcgcat gaac                        44


<210>  117
<211>  50
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Ct-L48P-G54R_rev

<400>  117
catgcgctcg atacggtgtc gaaactcatc agggttgaag ttgccgttgg                  50


<210>  118
<211>  44
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Ct-L48P-G54V_for

<400>  118
acttcaaccc tgatgagttt cgacacgtta tcgagcgcat gaac                        44


<210>  119
<211>  50
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Ct-L48P-G54V_rev

<400>  119
catgcgctcg ataacgtgtc gaaactcatc agggttgaag ttgccgttgg                  50


<210>  120
<211>  44
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Ct-L48F-G54C_for

<400>  120
acttcaactt cgatgagttt cgacactgta tcgagcgcat gaac                        44


<210>  121
<211>  50
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Ct-L48F-G54C_rev

<400>  121
catgcgctcg atacagtgtc gaaactcatc gaagttgaag ttgccgttgg                  50


<210>  122
<211>  44
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Ct-L48F-G54R_for

<400>  122
acttcaactt cgatgagttt cgacaccgta tcgagcgcat gaac                        44


<210>  123
<211>  50
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Ct-L48F-G54R_rev

<400>  123
catgcgctcg atacggtgtc gaaactcatc gaagttgaag ttgccgttgg                  50


<210>  124
<211>  44
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Ct-L48F-G54V_for

<400>  124
acttcaactt cgatgagttt cgacacgtta tcgagcgcat gaac                        44


<210>  125
<211>  50
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Ct-L48F-G54V_rev

<400>  125
catgcgctcg ataacgtgtc gaaactcatc gaagttgaag ttgccgttgg                  50


<210>  126
<211>  44
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Ct-L48P-F51V-G54V_for

<400>  126
acttcaaccc tgatgaggtt cgacacgtta tcgagcgcat gaac                        44


<210>  127
<211>  50
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Ct-L48P-F51V-G54V_rev

<400>  127
catgcgctcg ataacgtgtc gaacctcatc agggttgaag ttgccgttgg                  50


<210>  128
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Ct-L48F_for

<400>  128
caactttgat gagtttcgac ac                                                22


<210>  129
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Ct-L48F_rev

<400>  129
gtcgaaactc atcaaagttg aag                                               23


<210>  130
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Ct-F51L_for

<400>  130
cgatgagctt cgacacggc                                                    19


<210>  131
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Ct-F51L_rev

<400>  131
gccgtgtcga agctcatcg                                                    19


<210>  132
<211>  1638
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CtNHase_Insert for vector cloning


<220>
<221>  misc_feature
<222>  (1)..(36)
<223>  Overhang for Gibson cloning

<220>
<221>  misc_feature
<222>  (37)..(669)
<223>  alpha-subunit

<220>
<221>  misc_feature
<222>  (680)..(1336)
<223>  beta-subunit

<220>
<221>  misc_feature
<222>  (1389)..(1603)
<223>  accessory protein

<220>
<221>  misc_feature
<222>  (1604)..(1638)
<223>  Overhang for Gibson cloning

<400>  132
aataattttg tttaacttta agaaggagat atacatatgg ggcaatcaca cacgcatgac       60

caccatcacg acgggtacca ggcaccgccc gaagacatcg cgctgcgggt caaggccttg      120

gagtctctgc tgatcgagaa aggtcttgtc gacccagcgg ccatggactt ggtcgtccaa      180

acgtatgaac acaaggtagg cccccgaaac ggcgccaaag tcgtggccaa ggcctgggtg      240

gaccctgcct acaaggcccg tctgctggca gacggcactg ccggcattgc cgagctgggc      300

ttctccgggg tacagggcga ggacatggtc attctggaaa acacccccgc cgtccacaac      360

gtcgtcgttt gcaccttgtg ctcttgctac ccatggccga cgctgggctt gccccctgcc      420

tggtacaagg ccccgcccta ccggtcccgc atggtgagcg acccgcgtgg ggttctcgcg      480

gagttcggcc tggtgatccc cgcgaaggaa atccgcgtct gggacaccac ggccgaattg      540

cgctacatgg tgctgccgga acggcccgcg ggaactgaag cctacagcga agaacaactg      600

gccgaactcg ttacccgcga ttcgatgatc ggcaccggcc tgcccatcca acccacccca      660

tctcattaag gagttcgtca tgaatggcat tcacgatact gggggagcac atggttatgg      720

gccggtttac agagaaccga acgaacccgt ctttcgctac gactgggaaa aaacggtcat      780

gtccctgttc ccggcgctgt tcgccaacgg caacttcaac ctcgatgagt ttcgacacgg      840

catcgagcgc atgaacccca tcgactacct gaagggaacc tactacgaac actggatcca      900

ttccatcgaa accttgctgg tcgaaaaggg tgtgctcacg gcaacggaac tcgcgaccgg      960

caaggcatct ggcaagacag cgacaccggt gctgacgccg gccatcgtgg acggactgct     1020

cagcaccggg gcttctgccg cccgggagga gggtgcgcgg gcgcggttcg ctgtggggga     1080

caaggttcgc gtcctcaaca agaacccggt gggccatacc cgcatgccgc gctacacgcg     1140

gggcaaagtg gggacagtgg tcatcgacca tggtgtgttc gtgacgccgg acaccgcggc     1200

acacggaaag ggcgagcacc cccagcacgt ttacaccgtg agtttcacgt cggtcgaact     1260

gtgggggcaa gacgcctcct cgccgaagga cacgattcgc gtcgacttgt gggatgacta     1320

cctggagcca gcgtgatcat gaaagacgaa cggtttccat tgccagaggg ttcgctgaag     1380

gacctcgatg gccctgtgtt tgacgagcct tggcagtccc aggcgtttgc cttggtggtc     1440

agcatgcaca aggccggtct ctttcagtgg aaagactggg ccgagacctt caccgccgaa     1500

atcgacgctt ccccggctct gcccggcgaa agcgtcaacg acacctacta ccggcaatgg     1560

gtgtcggcgc tggaaaagtt ggtggcgtcg ctggggcttg taagcttggc tgttttggcg     1620

gatgagagaa gattttca                                                   1638


<210>  133
<211>  1834
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  KoNHase_Insert for vector cloning


<220>
<221>  misc_feature
<222>  (1)..(36)
<223>  Overhang for Gibson cloning

<220>
<221>  misc_feature
<222>  (37)..(645)
<223>  alpha-subunit

<220>
<221>  misc_feature
<222>  (661)..(1314)
<223>  beta-subunit

<220>
<221>  misc_feature
<222>  (1329)..(1799)
<223>  Accessory protein

<220>
<221>  misc_feature
<222>  (1800)..(1834)
<223>  Overhang for Gibson cloning

<400>  133
aataattttg tttaacttta agaaggagat atacatatga gccataaaca cgaccacgac       60

catacccacc cccccgttga tatcgagcta cgcgtccgcg cactggaatc cctgctgcag      120

gaaaaaggcc tgatcgaccc ggctgcgctg gatgagctga ttgacaccta cgagcacaaa      180

gtcggccccc gaaacggcgc acaggttgtc gccagagcgt ggagcgaccc ggaatacaaa      240

cgtcgactga tggaaaacgc cactgccgct attgctgaac tgggtttctc cggaatacag      300

ggcgaagaca tgctggtcgt ggagaacacg ccggacgtgc ataacgtcac cgtttgtacg      360

ctgtgttcct gctacccctg gccggtgctg ggtctgccgc cggtgtggta caaatcagcg      420

ccctatcgtt cgcgtatcgt catcgacccg cgcggcgttc tcgccgagtt cgggttacac      480

ataccagaaa acaaagagat tcgcgtctgg gacagcagcg ccgagctgcg ctatctggtc      540

ctgcctgaac gtccggcagg cacggaaggc tggagcgaag cgcagttgag cgaactcatc      600

acgcgcgatt cgatgattgg caccggtgtg gttagcgcac cataacgaaa ggagaaaacc      660

atgaacggga tacatgatct gggggggatg cacggccttg gcccgatccc taccgaggaa      720

aacgagccct atttccatca tgagtgggaa cgccgggtat ttcctctgtt cgcctcgttg      780

ttcgtcggcg gacattttaa cgtcgatgaa tttcgccacg ccatcgaacg tatggcgccg      840

accgaatatt tgcagtcgag ctactacgag cactggctgc atgcattcga aacgctgctg      900

ctggcaaagg gggcgatcac cgttgaagaa ctgtggggtg gcgcgaagcc tgccccttgc      960

aagcctggca cacctgtgct gacgcaggag atggtgtcga tggtggtcag caccggcggg     1020

tctgctcggg tcagtcacga tgttgcgccc cgcttccggg tgggcgattg ggtacgaacg     1080

aaaaatttca acccgaccac ccatacccgc ctgccacgct acgcacgcga taaagtcggt     1140

cgcatagaga tcgctcacgg tgtgtttatc acgccagata ctgcggcgca cgggctgggc     1200

gaacatcccc agcatgttta cagcgtcagt ttcaccgcgc aggcgctgtg gggagagccg     1260

cgccctgaca aagtgttcat cgatctgtgg gacgactatc tggaggaagc ataaaaggag     1320

atatacatat gaataatacg gtagcacaac acgattacgc cgccctcggg ttaccgcgcg     1380

atgaggaagg gccggtgttc gataagccct ggcaggcaaa agcgttctcc ctgatagtcc     1440

atctccaccg ggccgggctg ttcccgtggg cagaatgggt acagacattc agtaaagaga     1500

tcaacgcggc gccggcgcaa ccgggtgaaa gcgcgaatga tgcctactat cgtcagtgga     1560

cggcggcgat ggaaaacatg atgacggcac tcaacctgac ggtgccggat gaaatcaacc     1620

gacggacgca ggagtggcgc aaggcgtatc tcaacacgcc ccacggccag ccgattgtac     1680

tggcgaacgc cagttgcccg ccggcacata gccatcatca cctttcgccg ggcgtgccgg     1740

tcacggtgag tccggcactc tctatcaaca gcaaaatcga taatggagtt acaccataag     1800

cttggctgtt ttggcggatg agagaagatt ttca                                 1834


<210>  134
<211>  1855
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  NaNHase_Insert for vector cloning


<220>
<221>  misc_feature
<222>  (1)..(36)
<223>  Overhang for Gibson cloning

<220>
<221>  misc_feature
<222>  (37)..(720)
<223>  alpha-subunit

<220>
<221>  misc_feature
<222>  (791)..(1426)
<223>  beta-subunit

<220>
<221>  misc_feature
<222>  (1428)..(1820)
<223>  accessory protein

<220>
<221>  misc_feature
<222>  (1821)..(1855)
<223>  Overhang for Gibson cloning

<400>  134
aataattttg tttaacttta agaaggagat atacatatga acggcgtcca tgatcttggc       60

ggcaccgacg gcctcggccc ggtgatcacc gaggagaacg agccggtctg gcacagcgag      120

tgggagaagg ccgtcttcac gatgttcccc accaacttcg ccaagggaca tttcaacgtc      180

gactcgttcc gcttcgggat cgagaagatc catccggcgg actacctgtc gtcccggtac      240

tacgagcact ggctgcactc catcgagcat gcggtggtgg atcagggcgt cgtcgacgcc      300

gaggagctgg aggcgcgcac tcgtcactac ctcgagaacc ccgacgcgcc gttgccggac      360

cgccaggacc ctgatctggt gccactggtg gagacgatct cgcggggtgg tggctccgca      420

cgtcgcgaga gcgacaaggc gccgaggttc gaggtcggtg accgagtccg cgtcaagcgc      480

gacgaggtgc cccacggcca cacgcgccgc gcccgctacg tgcaggggcg cgaaggcgtg      540

atcgtccagg cccacggggc gttcatctac ccggacagcg caggcaacgg cgggccggag      600

gaccccgagc acgtctacac gatcctgttc gaggcctcgc acctgtgggg cgaacggacg      660

ggtgactcca acggaaccgt gaccttcgac gcgtgggagc cctacctcga gctggtctga      720

cggccagcgt cgtgccgtgt cgcgcactca gccacaacgg ccagcgatca cacatcaggg      780

agaagaaccg atgagccaga cccagagccc accccccgtc aacttcagcc tgccccgcac      840

ggaggagcag gtcgcggcgc gcgtgaaggc gctcgagtcg atcatgatcg agaagggcat      900

catgacgacg gacgccgtcg accgcctcgc cgagatctac gagaacgagg tcggaccaca      960

gctcggcgca agggtcgtgg cccgcgcctg gtctgacccc gacttccacg atcggctgct     1020

cgccaacgcc accgaggcct gcgaggagct ggacatcggc ggcctgcagg gcgaggacat     1080

ggtcgtcatc gagaacaccg acgacgtgca caacctcatc gtgtgcacgc tctgctcctg     1140

ctacccctgg ccaacgctcg gcctgccgcc caactggtac aagtaccccg cctaccgggc     1200

caaggccgtg cgcgaaccgc gggcgatgct gcgcgacgac ttcggcctcg acctgcccga     1260

ctcggttgag atccgcgtct gggataccag cgccgagctt cggtactggg tcctgcccaa     1320

gcgcccggaa ggtaccgaga gcatgacgga ggacgaattg gccgagctgg tcacccgcga     1380

ctccatgatc ggtgtcggcg tcgcccgtac ccccgaggcg gtctgacatg ccgacagcca     1440

cgacccccgt cggggatgcc ctgcggcacg tgcaggacct cctcgagcag ctgcccgagc     1500

gcgacaagtc cttcgacaag ccatgggagc ttcgagcctt cgccctggct gtcgcggcac     1560

acgacaacgg ccagtacgac tggagcgagt tccagcgctc gctcatcagc tccatcaagg     1620

cgtgggagca gggtggatcg ccagtgccgt ggcagtacta cgaccactgg ctgcaggccc     1680

tcgagaacgt gctggccgag accggcgccc tgaccgccga tgagctcgac gcccgcatgc     1740

acaccgtgct ctgtacgccc gtcaacagag accaccacga ggcgcgccac gacccagtgg     1800

ccatcgaccc cgcgcgctaa gcttggctgt tttggcggat gagagaagat tttca          1855


<210>  135
<211>  521
<212>  PRT
<213>  Rhodococcus erythropolis

<400>  135

Met Ala Thr Ile Arg Pro Asp Asp Lys Ala Ile Asp Ala Ala Ala Arg 
1               5                   10                  15      


His Tyr Gly Ile Thr Leu Asp Lys Thr Ala Arg Leu Glu Trp Pro Ala 
            20                  25                  30          


Leu Ile Asp Gly Ala Leu Gly Ser Tyr Asp Val Val Asp Gln Leu Tyr 
        35                  40                  45              


Ala Asp Glu Ala Thr Pro Pro Thr Thr Ser Arg Glu His Ala Val Pro 
    50                  55                  60                  


Ser Ala Ser Glu Asn Pro Leu Ser Ala Trp Tyr Val Thr Thr Ser Ile 
65                  70                  75                  80  


Pro Pro Thr Ser Asp Gly Val Leu Thr Gly Arg Arg Val Ala Ile Lys 
                85                  90                  95      


Asp Asn Val Thr Val Ala Gly Val Pro Met Met Asn Gly Ser Arg Thr 
            100                 105                 110         


Val Glu Gly Phe Thr Pro Ser Arg Asp Ala Thr Val Val Thr Arg Leu 
        115                 120                 125             


Leu Ala Ala Gly Ala Thr Val Ala Gly Lys Ala Val Cys Glu Asp Leu 
    130                 135                 140                 


Cys Phe Ser Gly Ser Ser Phe Thr Pro Ala Ser Gly Pro Val Arg Asn 
145                 150                 155                 160 


Pro Trp Asp Arg Gln Arg Glu Ala Gly Gly Ser Ser Gly Gly Ser Ala 
                165                 170                 175     


Ala Leu Val Ala Asn Gly Asp Val Asp Phe Ala Ile Gly Gly Asp Gln 
            180                 185                 190         


Gly Gly Ser Ile Arg Ile Pro Ala Ala Phe Cys Gly Val Val Gly His 
        195                 200                 205             


Lys Pro Thr Phe Gly Leu Val Pro Tyr Thr Gly Ala Phe Pro Ile Glu 
    210                 215                 220                 


Arg Thr Ile Asp His Leu Gly Pro Ile Thr Arg Thr Val His Asp Ala 
225                 230                 235                 240 


Ala Leu Met Leu Ser Val Ile Ala Gly Arg Asp Gly Asn Asp Pro Arg 
                245                 250                 255     


Gln Ala Asp Ser Val Glu Ala Gly Asp Tyr Leu Ser Thr Leu Asp Ser 
            260                 265                 270         


Asp Val Asp Gly Leu Arg Ile Gly Ile Val Arg Glu Gly Phe Gly His 
        275                 280                 285             


Ala Val Ser Gln Pro Glu Val Asp Asp Ala Val Arg Ala Ala Ala His 
    290                 295                 300                 


Ser Leu Thr Glu Ile Gly Cys Thr Val Glu Glu Val Asn Ile Pro Trp 
305                 310                 315                 320 


His Leu His Ala Phe His Ile Trp Asn Val Ile Ala Thr Asp Gly Gly 
                325                 330                 335     


Ala Tyr Gln Met Leu Asp Gly Asn Gly Tyr Gly Met Asn Ala Glu Gly 
            340                 345                 350         


Leu Tyr Asp Pro Glu Leu Met Ala His Phe Ala Ser Arg Arg Ile Gln 
        355                 360                 365             


His Ala Asp Ala Leu Ser Glu Thr Val Lys Leu Val Ala Leu Thr Gly 
    370                 375                 380                 


His His Gly Ile Thr Thr Leu Gly Gly Ala Ser Tyr Gly Lys Ala Arg 
385                 390                 395                 400 


Asn Leu Val Pro Leu Ala Arg Ala Ala Tyr Asp Thr Ala Leu Arg Gln 
                405                 410                 415     


Phe Asp Val Leu Val Met Pro Thr Leu Pro Tyr Val Ala Ser Glu Leu 
            420                 425                 430         


Pro Ala Lys Asp Val Asp Arg Ala Thr Phe Ile Thr Lys Ala Leu Gly 
        435                 440                 445             


Met Ile Ala Asn Thr Ala Pro Phe Asp Val Thr Gly His Pro Ser Leu 
    450                 455                 460                 


Ser Val Pro Ala Gly Leu Val Asn Gly Leu Pro Val Gly Met Met Ile 
465                 470                 475                 480 


Thr Gly Arg His Phe Asp Asp Ala Thr Val Leu Arg Val Gly Arg Ala 
                485                 490                 495     


Phe Glu Lys Leu Arg Gly Ala Phe Pro Thr Pro Ala Glu Arg Ala Ser 
            500                 505                 510         


Asn Ser Ala Pro Gln Leu Ser Pro Ala 
        515                 520     


<210>  136
<211>  216
<212>  DNA
<213>  Comamonas testosteroni


<220>
<221>  CDS
<222>  (1)..(216)

<400>  136
atg gcc ctg tgt ttg acg agc ctt ggc agt ccc agg cgt ttg cct tgg         48
Met Ala Leu Cys Leu Thr Ser Leu Gly Ser Pro Arg Arg Leu Pro Trp           
1               5                   10                  15                

tgg tca gca tgc aca agg ccg gtc tct ttc agt gga aag act ggg ccg         96
Trp Ser Ala Cys Thr Arg Pro Val Ser Phe Ser Gly Lys Thr Gly Pro           
            20                  25                  30                    

aga cct tca ccg ccg aaa tcg acg ctt ccc cgg ctc tgc ccg gcg aaa        144
Arg Pro Ser Pro Pro Lys Ser Thr Leu Pro Arg Leu Cys Pro Ala Lys           
        35                  40                  45                        

gcg tca acg aca cct act acc ggc aat ggg tgt cgg cgc tgg aaa agt        192
Ala Ser Thr Thr Pro Thr Thr Gly Asn Gly Cys Arg Arg Trp Lys Ser           
    50                  55                  60                            

tgg tgg cgt cgc tgg ggc ttg taa                                        216
Trp Trp Arg Arg Trp Gly Leu                                               
65                  70                                                    


<210>  137
<211>  71
<212>  PRT
<213>  Comamonas testosteroni

<400>  137

Met Ala Leu Cys Leu Thr Ser Leu Gly Ser Pro Arg Arg Leu Pro Trp 
1               5                   10                  15      


Trp Ser Ala Cys Thr Arg Pro Val Ser Phe Ser Gly Lys Thr Gly Pro 
            20                  25                  30          


Arg Pro Ser Pro Pro Lys Ser Thr Leu Pro Arg Leu Cys Pro Ala Lys 
        35                  40                  45              


Ala Ser Thr Thr Pro Thr Thr Gly Asn Gly Cys Arg Arg Trp Lys Ser 
    50                  55                  60                  


Trp Trp Arg Arg Trp Gly Leu 
65                  70      


<210>  138
<211>  471
<212>  DNA
<213>  Klebsiella oxytoca


<220>
<221>  CDS
<222>  (1)..(471)

<400>  138
atg aat aat acg gta gca caa cac gat tac gcc gcc ctc ggg tta ccg         48
Met Asn Asn Thr Val Ala Gln His Asp Tyr Ala Ala Leu Gly Leu Pro           
1               5                   10                  15                

cgc gat gag gaa ggg ccg gtg ttc gat aag ccc tgg cag gca aaa gcg         96
Arg Asp Glu Glu Gly Pro Val Phe Asp Lys Pro Trp Gln Ala Lys Ala           
            20                  25                  30                    

ttc tcc ctg ata gtc cat ctc cac cgg gcc ggg ctg ttc ccg tgg gca        144
Phe Ser Leu Ile Val His Leu His Arg Ala Gly Leu Phe Pro Trp Ala           
        35                  40                  45                        

gaa tgg gta cag aca ttc agt aaa gag atc aac gcg gcg ccg gcg caa        192
Glu Trp Val Gln Thr Phe Ser Lys Glu Ile Asn Ala Ala Pro Ala Gln           
    50                  55                  60                            

ccg ggt gaa agc gcg aat gat gcc tac tat cgt cag tgg acg gcg gcg        240
Pro Gly Glu Ser Ala Asn Asp Ala Tyr Tyr Arg Gln Trp Thr Ala Ala           
65                  70                  75                  80            

atg gaa aac atg atg acg gca ctc aac ctg acg gtg ccg gat gaa atc        288
Met Glu Asn Met Met Thr Ala Leu Asn Leu Thr Val Pro Asp Glu Ile           
                85                  90                  95                

aac cga cgg acg cag gag tgg cgc aag gcg tat ctc aac acg ccc cac        336
Asn Arg Arg Thr Gln Glu Trp Arg Lys Ala Tyr Leu Asn Thr Pro His           
            100                 105                 110                   

ggc cag ccg att gta ctg gcg aac gcc agt tgc ccg ccg gca cat agc        384
Gly Gln Pro Ile Val Leu Ala Asn Ala Ser Cys Pro Pro Ala His Ser           
        115                 120                 125                       

cat cat cac ctt tcg ccg ggc gtg ccg gtc acg gtg agt ccg gca ctc        432
His His His Leu Ser Pro Gly Val Pro Val Thr Val Ser Pro Ala Leu           
    130                 135                 140                           

tct atc aac agc aaa atc gat aat gga gtt aca cca taa                    471
Ser Ile Asn Ser Lys Ile Asp Asn Gly Val Thr Pro                           
145                 150                 155                               


<210>  139
<211>  156
<212>  PRT
<213>  Klebsiella oxytoca

<400>  139

Met Asn Asn Thr Val Ala Gln His Asp Tyr Ala Ala Leu Gly Leu Pro 
1               5                   10                  15      


Arg Asp Glu Glu Gly Pro Val Phe Asp Lys Pro Trp Gln Ala Lys Ala 
            20                  25                  30          


Phe Ser Leu Ile Val His Leu His Arg Ala Gly Leu Phe Pro Trp Ala 
        35                  40                  45              


Glu Trp Val Gln Thr Phe Ser Lys Glu Ile Asn Ala Ala Pro Ala Gln 
    50                  55                  60                  


Pro Gly Glu Ser Ala Asn Asp Ala Tyr Tyr Arg Gln Trp Thr Ala Ala 
65                  70                  75                  80  


Met Glu Asn Met Met Thr Ala Leu Asn Leu Thr Val Pro Asp Glu Ile 
                85                  90                  95      


Asn Arg Arg Thr Gln Glu Trp Arg Lys Ala Tyr Leu Asn Thr Pro His 
            100                 105                 110         


Gly Gln Pro Ile Val Leu Ala Asn Ala Ser Cys Pro Pro Ala His Ser 
        115                 120                 125             


His His His Leu Ser Pro Gly Val Pro Val Thr Val Ser Pro Ala Leu 
    130                 135                 140                 


Ser Ile Asn Ser Lys Ile Asp Asn Gly Val Thr Pro 
145                 150                 155     


<210>  140
<211>  393
<212>  DNA
<213>  Nitriliruptor alkaliphilus


<220>
<221>  CDS
<222>  (1)..(393)

<400>  140
atg ccg aca gcc acg acc ccc gtc ggg gat gcc ctg cgg cac gtg cag         48
Met Pro Thr Ala Thr Thr Pro Val Gly Asp Ala Leu Arg His Val Gln           
1               5                   10                  15                

gac ctc ctc gag cag ctg ccc gag cgc gac aag tcc ttc gac aag cca         96
Asp Leu Leu Glu Gln Leu Pro Glu Arg Asp Lys Ser Phe Asp Lys Pro           
            20                  25                  30                    

tgg gag ctt cga gcc ttc gcc ctg gct gtc gcg gca cac gac aac ggc        144
Trp Glu Leu Arg Ala Phe Ala Leu Ala Val Ala Ala His Asp Asn Gly           
        35                  40                  45                        

cag tac gac tgg agc gag ttc cag cgc tcg ctc atc agc tcc atc aag        192
Gln Tyr Asp Trp Ser Glu Phe Gln Arg Ser Leu Ile Ser Ser Ile Lys           
    50                  55                  60                            

gcg tgg gag cag ggt gga tcg cca gtg ccg tgg cag tac tac gac cac        240
Ala Trp Glu Gln Gly Gly Ser Pro Val Pro Trp Gln Tyr Tyr Asp His           
65                  70                  75                  80            

tgg ctg cag gcc ctc gag aac gtg ctg gcc gag acc ggc gcc ctg acc        288
Trp Leu Gln Ala Leu Glu Asn Val Leu Ala Glu Thr Gly Ala Leu Thr           
                85                  90                  95                

gcc gat gag ctc gac gcc cgc atg cac acc gtg ctc tgt acg ccc gtc        336
Ala Asp Glu Leu Asp Ala Arg Met His Thr Val Leu Cys Thr Pro Val           
            100                 105                 110                   

aac aga gac cac cac gag gcg cgc cac gac cca gtg gcc atc gac ccc        384
Asn Arg Asp His His Glu Ala Arg His Asp Pro Val Ala Ile Asp Pro           
        115                 120                 125                       

gcg cgc taa                                                            393
Ala Arg                                                                   
    130                                                                   


<210>  141
<211>  130
<212>  PRT
<213>  Nitriliruptor alkaliphilus

<400>  141

Met Pro Thr Ala Thr Thr Pro Val Gly Asp Ala Leu Arg His Val Gln 
1               5                   10                  15      


Asp Leu Leu Glu Gln Leu Pro Glu Arg Asp Lys Ser Phe Asp Lys Pro 
            20                  25                  30          


Trp Glu Leu Arg Ala Phe Ala Leu Ala Val Ala Ala His Asp Asn Gly 
        35                  40                  45              


Gln Tyr Asp Trp Ser Glu Phe Gln Arg Ser Leu Ile Ser Ser Ile Lys 
    50                  55                  60                  


Ala Trp Glu Gln Gly Gly Ser Pro Val Pro Trp Gln Tyr Tyr Asp His 
65                  70                  75                  80  


Trp Leu Gln Ala Leu Glu Asn Val Leu Ala Glu Thr Gly Ala Leu Thr 
                85                  90                  95      


Ala Asp Glu Leu Asp Ala Arg Met His Thr Val Leu Cys Thr Pro Val 
            100                 105                 110         


Asn Arg Asp His His Glu Ala Arg His Asp Pro Val Ala Ile Asp Pro 
        115                 120                 125             


Ala Arg 
    130 


<210>  142
<211>  1218
<212>  DNA
<213>  Gordona hydrophobica


<220>
<221>  CDS
<222>  (1)..(1218)

<400>  142
atg acc gac aac cgg ctt ccc gtc acc gtg ctc tcc ggc ttc ctc ggc         48
Met Thr Asp Asn Arg Leu Pro Val Thr Val Leu Ser Gly Phe Leu Gly           
1               5                   10                  15                

gcc ggt aag acg acc ctg ctc aac cga gtg ctt cac aac cgc gac ggc         96
Ala Gly Lys Thr Thr Leu Leu Asn Arg Val Leu His Asn Arg Asp Gly           
            20                  25                  30                    

cgc cgg atc gcc gtc gtc gtc aac gac atg agc gag gtg aac atc gac        144
Arg Arg Ile Ala Val Val Val Asn Asp Met Ser Glu Val Asn Ile Asp           
        35                  40                  45                        

agc gcc gag atc gag cgc gag gtg acg ttg agc cgg tcc cag gag aag        192
Ser Ala Glu Ile Glu Arg Glu Val Thr Leu Ser Arg Ser Gln Glu Lys           
    50                  55                  60                            

atc gtc gag atg tcc aac ggg tgc atc tgt tgc acc ctg cga gaa gac        240
Ile Val Glu Met Ser Asn Gly Cys Ile Cys Cys Thr Leu Arg Glu Asp           
65                  70                  75                  80            

ctg ctc gtg gag atc acc gaa ctc gca gcg aag ggg tcc ttc gac tac        288
Leu Leu Val Glu Ile Thr Glu Leu Ala Ala Lys Gly Ser Phe Asp Tyr           
                85                  90                  95                

ctc ctc atc gag tcg tcc ggc atc tcc gaa cca ctg ccg gtg gcc gag        336
Leu Leu Ile Glu Ser Ser Gly Ile Ser Glu Pro Leu Pro Val Ala Glu           
            100                 105                 110                   

acg ttc acg ttc gtc gac acc gac ggc aac gca ttg tcg gat gtg gca        384
Thr Phe Thr Phe Val Asp Thr Asp Gly Asn Ala Leu Ser Asp Val Ala           
        115                 120                 125                       

cgg ctg gac acc atg gtc acc gtt gtg gac ggc tac agc ttc ctc cgc        432
Arg Leu Asp Thr Met Val Thr Val Val Asp Gly Tyr Ser Phe Leu Arg           
    130                 135                 140                           

gat ttc cga tct ggc ggc gac atc gtg gcc gag gcg ccg gag gat cag        480
Asp Phe Arg Ser Gly Gly Asp Ile Val Ala Glu Ala Pro Glu Asp Gln           
145                 150                 155                 160           

cgc gac ttg tcg gat ctg ctc gtc gat cag gtc gag ttc gcc gac gtc        528
Arg Asp Leu Ser Asp Leu Leu Val Asp Gln Val Glu Phe Ala Asp Val           
                165                 170                 175               

atc ctg gtc agc aag gcc gac ttg atc aac gca gcc gag ctg gcc gag        576
Ile Leu Val Ser Lys Ala Asp Leu Ile Asn Ala Ala Glu Leu Ala Glu           
            180                 185                 190                   

ctg acg gcg gtg ctg cgt tcg ctc aac gcc acg gcc cgg atc gag ccg        624
Leu Thr Ala Val Leu Arg Ser Leu Asn Ala Thr Ala Arg Ile Glu Pro           
        195                 200                 205                       

atg atc gac ggc gac gtc ccc ctc gac acc atc atg gac acc ggg agg        672
Met Ile Asp Gly Asp Val Pro Leu Asp Thr Ile Met Asp Thr Gly Arg           
    210                 215                 220                           

ttc tcc ctg gtg aag gcc gca cag gcg ccc ggc tgg ctg cag gag ttg        720
Phe Ser Leu Val Lys Ala Ala Gln Ala Pro Gly Trp Leu Gln Glu Leu           
225                 230                 235                 240           

cgg ggc acc cat ata ccc gag acg ttg gag tac gga gtc agc tcg gcg        768
Arg Gly Thr His Ile Pro Glu Thr Leu Glu Tyr Gly Val Ser Ser Ala           
                245                 250                 255               

gtg tat cgg gag cgc gcg ccg ttc cac ccg gtc agg ttc cac gac ttc        816
Val Tyr Arg Glu Arg Ala Pro Phe His Pro Val Arg Phe His Asp Phe           
            260                 265                 270                   

ctc acc gcg gag tgg acg aac ggt gtg ctg ctg cgc gcc aag ggc tat        864
Leu Thr Ala Glu Trp Thr Asn Gly Val Leu Leu Arg Ala Lys Gly Tyr           
        275                 280                 285                       

ttc tgg aat gcg gct cgg atg act gag atc ggc agt gtc tcg cag gcc        912
Phe Trp Asn Ala Ala Arg Met Thr Glu Ile Gly Ser Val Ser Gln Ala           
    290                 295                 300                           

ggg cat ctc atc cgc cac ggc tac atc ggg cga tgg tgg cag ttc ctg        960
Gly His Leu Ile Arg His Gly Tyr Ile Gly Arg Trp Trp Gln Phe Leu           
305                 310                 315                 320           

ccg ccg agc tac tgg ccc gac gac gag gag cgg cgc acc gcg att ctc       1008
Pro Pro Ser Tyr Trp Pro Asp Asp Glu Glu Arg Arg Thr Ala Ile Leu           
                325                 330                 335               

gag aag tgg gaa gat ccc gtc ggt gac tgc cgc cag gag atc gtg ttc       1056
Glu Lys Trp Glu Asp Pro Val Gly Asp Cys Arg Gln Glu Ile Val Phe           
            340                 345                 350                   

atc gga cag gga atc gac tgg gac gtc ctg ttc gcc gct cta gac gcc       1104
Ile Gly Gln Gly Ile Asp Trp Asp Val Leu Phe Ala Ala Leu Asp Ala           
        355                 360                 365                       

tgt ctg ctg acc cag gag gag atc gaa ctc ggc ccg gac gcg tgg gaa       1152
Cys Leu Leu Thr Gln Glu Glu Ile Glu Leu Gly Pro Asp Ala Trp Glu           
    370                 375                 380                           

cag tgg ccg gac cca ctc ggc gac ggc gac gcc gcc ggt gtc aca tcg       1200
Gln Trp Pro Asp Pro Leu Gly Asp Gly Asp Ala Ala Gly Val Thr Ser           
385                 390                 395                 400           

aag atc gca cag gca taa                                               1218
Lys Ile Ala Gln Ala                                                       
                405                                                       


<210>  143
<211>  405
<212>  PRT
<213>  Gordona hydrophobica

<400>  143

Met Thr Asp Asn Arg Leu Pro Val Thr Val Leu Ser Gly Phe Leu Gly 
1               5                   10                  15      


Ala Gly Lys Thr Thr Leu Leu Asn Arg Val Leu His Asn Arg Asp Gly 
            20                  25                  30          


Arg Arg Ile Ala Val Val Val Asn Asp Met Ser Glu Val Asn Ile Asp 
        35                  40                  45              


Ser Ala Glu Ile Glu Arg Glu Val Thr Leu Ser Arg Ser Gln Glu Lys 
    50                  55                  60                  


Ile Val Glu Met Ser Asn Gly Cys Ile Cys Cys Thr Leu Arg Glu Asp 
65                  70                  75                  80  


Leu Leu Val Glu Ile Thr Glu Leu Ala Ala Lys Gly Ser Phe Asp Tyr 
                85                  90                  95      


Leu Leu Ile Glu Ser Ser Gly Ile Ser Glu Pro Leu Pro Val Ala Glu 
            100                 105                 110         


Thr Phe Thr Phe Val Asp Thr Asp Gly Asn Ala Leu Ser Asp Val Ala 
        115                 120                 125             


Arg Leu Asp Thr Met Val Thr Val Val Asp Gly Tyr Ser Phe Leu Arg 
    130                 135                 140                 


Asp Phe Arg Ser Gly Gly Asp Ile Val Ala Glu Ala Pro Glu Asp Gln 
145                 150                 155                 160 


Arg Asp Leu Ser Asp Leu Leu Val Asp Gln Val Glu Phe Ala Asp Val 
                165                 170                 175     


Ile Leu Val Ser Lys Ala Asp Leu Ile Asn Ala Ala Glu Leu Ala Glu 
            180                 185                 190         


Leu Thr Ala Val Leu Arg Ser Leu Asn Ala Thr Ala Arg Ile Glu Pro 
        195                 200                 205             


Met Ile Asp Gly Asp Val Pro Leu Asp Thr Ile Met Asp Thr Gly Arg 
    210                 215                 220                 


Phe Ser Leu Val Lys Ala Ala Gln Ala Pro Gly Trp Leu Gln Glu Leu 
225                 230                 235                 240 


Arg Gly Thr His Ile Pro Glu Thr Leu Glu Tyr Gly Val Ser Ser Ala 
                245                 250                 255     


Val Tyr Arg Glu Arg Ala Pro Phe His Pro Val Arg Phe His Asp Phe 
            260                 265                 270         


Leu Thr Ala Glu Trp Thr Asn Gly Val Leu Leu Arg Ala Lys Gly Tyr 
        275                 280                 285             


Phe Trp Asn Ala Ala Arg Met Thr Glu Ile Gly Ser Val Ser Gln Ala 
    290                 295                 300                 


Gly His Leu Ile Arg His Gly Tyr Ile Gly Arg Trp Trp Gln Phe Leu 
305                 310                 315                 320 


Pro Pro Ser Tyr Trp Pro Asp Asp Glu Glu Arg Arg Thr Ala Ile Leu 
                325                 330                 335     


Glu Lys Trp Glu Asp Pro Val Gly Asp Cys Arg Gln Glu Ile Val Phe 
            340                 345                 350         


Ile Gly Gln Gly Ile Asp Trp Asp Val Leu Phe Ala Ala Leu Asp Ala 
        355                 360                 365             


Cys Leu Leu Thr Gln Glu Glu Ile Glu Leu Gly Pro Asp Ala Trp Glu 
    370                 375                 380                 


Gln Trp Pro Asp Pro Leu Gly Asp Gly Asp Ala Ala Gly Val Thr Ser 
385                 390                 395                 400 


Lys Ile Ala Gln Ala 
                405 


<210>  144
<211>  146
<212>  PRT
<213>  Pseudomonas marginalis

<400>  144

Met Asn Thr Ser Met Arg His Asp Tyr Lys Ala Val Gly Leu Pro Leu 
1               5                   10                  15      


Asp Asp Glu Gly Pro Val Phe Asp Lys Pro Trp Gln Ala Gln Ala Phe 
            20                  25                  30          


Ser Leu Leu Val His Leu His Gln Ala Gly Val Phe Pro Trp Lys Asp 
        35                  40                  45              


Trp Val Gln Val Phe Ser Glu Glu Ile Lys Ala Ala Pro Ala Gln Pro 
    50                  55                  60                  


Gly Glu Gly Val Asn Asp Ala Tyr Tyr Arg Gln Trp Ile Thr Ala Met 
65                  70                  75                  80  


Glu Arg Met Val Thr Thr Leu Gly Leu Thr Gly Met Glu Asp Ile Val 
                85                  90                  95      


Gln Arg Gly Glu Glu Trp Arg Gln Ala Tyr Leu Asn Thr Pro His Gly 
            100                 105                 110         


Gln Pro Val Val Leu Leu Asn Ala Ser Cys Pro Pro Ala His Gly His 
        115                 120                 125             


Thr Gly Glu His Leu Pro His Arg Glu Pro Val Ala Ile Ser Arg Ala 
    130                 135                 140                 


Ser Asn 
145     


<210>  145
<211>  399
<212>  PRT
<213>  Rhodococcus erythropolis

<400>  145

Met Val Asp Thr Arg Leu Pro Val Thr Val Leu Ser Gly Phe Leu Gly 
1               5                   10                  15      


Ala Gly Lys Thr Thr Leu Leu Asn Glu Ile Leu Arg Asn Arg Glu Gly 
            20                  25                  30          


Arg Arg Val Ala Val Ile Val Asn Asp Met Ser Glu Ile Asn Ile Asp 
        35                  40                  45              


Ser Ala Glu Val Glu Arg Glu Ile Ser Leu Ser Arg Ser Glu Glu Lys 
    50                  55                  60                  


Leu Val Glu Met Thr Asn Gly Cys Ile Cys Cys Thr Leu Arg Glu Asp 
65                  70                  75                  80  


Leu Leu Ser Glu Ile Ser Ala Leu Ala Ala Asp Gly Arg Phe Asp Tyr 
                85                  90                  95      


Leu Leu Ile Glu Ser Ser Gly Ile Ser Glu Pro Leu Pro Val Ala Glu 
            100                 105                 110         


Thr Phe Thr Phe Ile Asp Thr Asp Gly His Ala Leu Ala Asp Val Ala 
        115                 120                 125             


Arg Leu Asp Thr Met Val Thr Val Val Asp Gly His Ser Phe Leu Arg 
    130                 135                 140                 


Asp Tyr Thr Ala Gly Gly Arg Val Glu Ala Asp Ala Pro Glu Asp Glu 
145                 150                 155                 160 


Arg Asp Ile Ala Asp Leu Leu Val Asp Gln Ile Glu Phe Ala Asp Val 
                165                 170                 175     


Ile Leu Val Ser Lys Ala Asp Leu Val Ser His Gln His Leu Val Glu 
            180                 185                 190         


Leu Thr Ala Val Leu Arg Ser Leu Asn Ala Ser Ala Ala Ile Val Pro 
        195                 200                 205             


Met Thr Leu Gly Arg Ile Pro Leu Asp Thr Ile Leu Asp Thr Gly Leu 
    210                 215                 220                 


Phe Ser Leu Glu Lys Ala Ala Gln Ala Pro Gly Trp Leu Gln Glu Leu 
225                 230                 235                 240 


Gln Gly Glu His Ile Pro Glu Thr Glu Glu Tyr Gly Ile Gly Ser Val 
                245                 250                 255     


Val Tyr Arg Glu Arg Ala Pro Phe His Pro Gln Arg Leu His Asp Phe 
            260                 265                 270         


Leu Ser Ser Glu Trp Thr Asn Gly Lys Leu Leu Arg Ala Lys Gly Tyr 
        275                 280                 285             


Tyr Trp Asn Ala Gly Arg Phe Thr Glu Ile Gly Ser Ile Ser Gln Ala 
    290                 295                 300                 


Gly His Leu Ile Arg His Gly Tyr Val Gly Arg Trp Trp Lys Phe Leu 
305                 310                 315                 320 


Pro Arg Asp Glu Trp Pro Ala Asp Asp Tyr Arg Arg Asp Gly Ile Leu 
                325                 330                 335     


Asp Lys Trp Glu Glu Pro Val Gly Asp Cys Arg Gln Glu Leu Val Phe 
            340                 345                 350         


Ile Gly Gln Ala Ile Asp Pro Ser Arg Leu His Arg Glu Leu Asp Ala 
        355                 360                 365             


Cys Leu Leu Thr Thr Ala Glu Ile Glu Leu Gly Pro Asp Val Trp Thr 
    370                 375                 380                 


Thr Trp Ser Asp Pro Leu Gly Val Gly Tyr Thr Asp Gln Thr Val 
385                 390                 395                 


