               SEQUENCE LISTING

<110> FABMID
      PICHUGIN Andrey

<120> METHODS FOR CIRCULARIZING LINEAR DOUBLE STRANDED NUCLEIC ACIDS

<130> IBIO - 1345/PCT

<150> EP20305391.3
<151> 2020-04-22

<160> 45

<170> BiSSAP 1.3.6

<210> 1
<211> 15
<212> PRT
<213> Artificial Sequence


<220> 
<223> Antennapedia Penetratin hydrophilic CPP

<400> 1
Arg Gln Ile Lys Trp Phe Gln Asn Arg Arg Met Lys Trp Lys Lys 
1               5                   10                  15  

<210> 2
<211> 19
<212> PRT
<213> Artificial Sequence


<220> 
<223> BMV Gag-(7-25) hydrophilic CPP

<400> 2
Lys Met Thr Arg Ala Gln Arg Arg Ala Ala Ala Arg Arg Asn Arg Trp 
1               5                   10                  15      
Thr Ala Arg 
            

<210> 3
<211> 13
<212> PRT
<213> Artificial Sequence


<220> 
<223> D-Tat hydrophilic CPP

<400> 3
Gly Arg Lys Lys Arg Arg Gln Arg Arg Arg Pro Pro Gln 
1               5                   10              

<210> 4
<211> 15
<212> PRT
<213> Artificial Sequence


<220> 
<223> FHV Coat-(35-49) hydrophilic CPP

<400> 4
Arg Arg Arg Arg Asn Arg Thr Arg Arg Asn Arg Arg Arg Val Arg 
1               5                   10                  15  

<210> 5
<211> 13
<212> PRT
<213> Artificial Sequence


<220> 
<223> HTLV-II Rex-(4-16) hydrophilic CPP

<400> 5
Thr Arg Arg Gln Arg Thr Arg Arg Ala Arg Arg Asn Arg 
1               5                   10              

<210> 6
<211> 12
<212> PRT
<213> Artificial Sequence


<220> 
<223> PTD-4 hydrophilic CPP

<400> 6
Pro Ile Arg Arg Arg Lys Lys Leu Arg Arg Leu Lys 
1               5                   10          

<210> 7
<211> 12
<212> PRT
<213> Artificial Sequence


<220> 
<223> PTD-5 hydrophilic CPP

<400> 7
Arg Arg Gln Arg Arg Thr Ser Lys Leu Met Lys Arg 
1               5                   10          

<210> 8
<211> 13
<212> PRT
<213> Artificial Sequence


<220> 
<223> R9-Tat hydrophilic CPP

<400> 8
Gly Arg Arg Arg Arg Arg Arg Arg Arg Arg Pro Pro Gln 
1               5                   10              

<210> 9
<211> 18
<212> PRT
<213> Artificial Sequence


<220> 
<223> SynB1 hydrophilic CPP

<400> 9
Arg Gly Gly Arg Leu Ser Tyr Ser Arg Arg Arg Phe Ser Thr Ser Thr 
1               5                   10                  15      
Gly Arg 
        

<210> 10
<211> 10
<212> PRT
<213> Artificial Sequence


<220> 
<223> SynB3 hydrophilic CPP

<400> 10
Arg Arg Leu Ser Tyr Ser Arg Arg Arg Phe 
1               5                   10  

<210> 11
<211> 11
<212> PRT
<213> Artificial Sequence


<220> 
<223> TAT hydrophilic CPP

<400> 11
Tyr Gly Arg Lys Lys Arg Arg Gln Arg Arg Arg 
1               5                   10      

<210> 12
<211> 27
<212> PRT
<213> Artificial Sequence


<220> 
<223> Transportan amphipathic CPP

<400> 12
Gly Trp Thr Leu Asn Ser Ala Gly Tyr Leu Leu Gly Lys Ile Asn Leu 
1               5                   10                  15      
Lys Ala Leu Ala Ala Leu Ala Lys Lys Ile Leu 
            20                  25          

<210> 13
<211> 17
<212> PRT
<213> Artificial Sequence


<220> 
<223> MAP amphipathic CPP

<400> 13
Lys Leu Ala Leu Lys Leu Ala Leu Lys Leu Ala Leu Ala Leu Lys Leu 
1               5                   10                  15      
Ala 
    

<210> 14
<211> 27
<212> PRT
<213> Artificial Sequence


<220> 
<223> SBP amphipathic CPP

<400> 14
Met Gly Leu Gly Leu His Leu Leu Val Leu Ala Ala Ala Leu Gln Gly 
1               5                   10                  15      
Ala Trp Ser Gln Pro Lys Lys Lys Arg Lys Val 
            20                  25          

<210> 15
<211> 27
<212> PRT
<213> Artificial Sequence


<220> 
<223> FBP amphipathic CPP

<400> 15
Gly Ala Leu Phe Leu Gly Trp Leu Gly Ala Ala Gly Ser Thr Met Gly 
1               5                   10                  15      
Ala Trp Ser Gln Pro Lys Lys Lys Arg Lys Val 
            20                  25          

<210> 16
<211> 27
<212> PRT
<213> Artificial Sequence


<220> 
<223> MPG amphipathic CPP

<400> 16
Gly Ala Leu Phe Leu Gly Phe Leu Gly Ala Ala Gly Ser Thr Met Gly 
1               5                   10                  15      
Ala Trp Ser Gln Pro Lys Lys Lys Arg Lys Val 
            20                  25          

<210> 17
<211> 27
<212> PRT
<213> Artificial Sequence


<220> 
<223> MPG(ΔNLS) amphipathic CPP

<400> 17
Gly Ala Leu Phe Leu Gly Phe Leu Gly Ala Ala Gly Ser Thr Met Gly 
1               5                   10                  15      
Ala Trp Ser Gln Pro Lys Ser Lys Arg Lys Val 
            20                  25          

<210> 18
<211> 21
<212> PRT
<213> Artificial Sequence


<220> 
<223> Pep-1 amphipathic CPP

<400> 18
Lys Glu Thr Trp Trp Glu Thr Trp Trp Thr Glu Trp Ser Gln Pro Lys 
1               5                   10                  15      
Lys Lys Arg Lys Val 
            20      

<210> 19
<211> 21
<212> PRT
<213> Artificial Sequence


<220> 
<223> Pep-2 amphipathic CPP

<400> 19
Lys Glu Thr Trp Phe Glu Thr Trp Phe Thr Glu Trp Ser Gln Pro Lys 
1               5                   10                  15      
Lys Lys Arg Lys Val 
            20      

<210> 20
<211> 9
<212> PRT
<213> Artificial Sequence


<220> 
<223> nuclear localization domain of c-Myc

<400> 20
Pro Ala Ala Lys Arg Val Lys Leu Asp 
1               5                   

<210> 21
<211> 25
<212> PRT
<213> Artificial Sequence


<220> 
<223> nuclear localization domain of EGL-13

<400> 21
Met Ser Arg Arg Arg Lys Ala Asn Pro Thr Lys Leu Ser Glu Asn Ala 
1               5                   10                  15      
Lys Lys Leu Ala Lys Glu Val Glu Asn 
            20                  25  

<210> 22
<211> 10
<212> PRT
<213> Artificial Sequence


<220> 
<223> nuclear localization domain of nucleoplasmin #1

<400> 22
Pro Ala Ala Thr Lys Lys Ala Gly Gln Ala 
1               5                   10  

<210> 23
<211> 16
<212> PRT
<213> Artificial Sequence


<220> 
<223> nuclear localization domain of nucleoplasmin #2

<400> 23
Lys Arg Pro Ala Ala Thr Lys Lys Ala Gly Gln Ala Lys Lys Lys Lys 
1               5                   10                  15      



<210> 24
<211> 7
<212> PRT
<213> Artificial Sequence


<220> 
<223> nuclear localization domain of SV40

<400> 24
Pro Lys Lys Lys Arg Lys Val 
1               5           

<210> 25
<211> 29
<212> PRT
<213> Artificial Sequence


<220> 
<223> ER localization domain

<400> 25
Met Met Ser Phe Val Ser Leu Leu Leu Val Gly Ile Leu Phe Trp Ala 
1               5                   10                  15      
Thr Glu Ala Glu Gln Leu Thr Lys Cys Glu Val Phe Gln 
            20                  25                  

<210> 26
<211> 25
<212> PRT
<213> Artificial Sequence


<220> 
<223> mitochondria localization domain

<400> 26
Met Leu Ser Leu Arg Gln Ser Ile Arg Phe Phe Lys Pro Ala Thr Arg 
1               5                   10                  15      
Thr Leu Cys Ser Ser Arg Tyr Leu Leu 
            20                  25  

<210> 27
<211> 39
<212> PRT
<213> Artificial Sequence


<220> 
<223> chloroplast localization domain

<400> 27
Met Val Ala Met Ala Met Ala Ser Leu Gln Ser Ser Met Ser Ser Leu 
1               5                   10                  15      
Ser Leu Ser Ser Asn Ser Phe Leu Gly Gln Pro Leu Ser Pro Ile Thr 
            20                  25                  30          
Leu Ser Pro Phe Leu Gln Gly 
        35                  

<210> 28
<211> 13
<212> PRT
<213> Artificial Sequence


<220> 
<223> peroxisome localization domain

<220> 
<221> VARIANT
<222> 1
<223> X represents any amino acid residue

<220> 
<221> VARIANT
<222> 2
<223> X represents any amino acid residue

<220> 
<221> VARIANT
<222> 3
<223> X represents any amino acid residue

<220> 
<221> VARIANT
<222> 4
<223> X represents any amino acid residue

<220> 
<221> VARIANT
<222> 7
<223> X represents any amino acid residue

<220> 
<221> VARIANT
<222> 8
<223> X represents any amino acid residue

<220> 
<221> VARIANT
<222> 9
<223> X represents any amino acid residue

<220> 
<221> VARIANT
<222> 10
<223> X represents any amino acid residue

<220> 
<221> VARIANT
<222> 11
<223> X represents any amino acid residue

<400> 28
Xaa Xaa Xaa Xaa Arg Leu Xaa Xaa Xaa Xaa Xaa His Leu 
1               5                   10              

<210> 29
<211> 16
<212> PRT
<213> Artificial Sequence


<220> 
<223> vacuole localization domain #1

<400> 29
His Ser Arg Phe Asn Pro Ile Arg Leu Pro Thr Thr His Glu Pro Ala 
1               5                   10                  15      



<210> 30
<211> 23
<212> PRT
<213> Artificial Sequence


<220> 
<223> vacuole localization domain #2

<400> 30
Ser Ser Ser Ser Phe Ala Asp Ser Asn Pro Ile Arg Pro Val Thr Asp 
1               5                   10                  15      
Arg Ala Ala Ser Thr Leu Glu 
            20              

<210> 31
<211> 15
<212> PRT
<213> Artificial Sequence


<220> 
<223> vacuole localization domain #3

<400> 31
Val Phe Ala Glu Ala Ile Ala Ala Asn Ser Thr Leu Val Ala Glu 
1               5                   10                  15  

<210> 32
<211> 8
<212> PRT
<213> Artificial Sequence


<220> 
<223> vacuole localization domain #4

<400> 32
Asn Gly Leu Leu Val Asp Thr Met 
1               5               

<210> 33
<211> 22
<212> PRT
<213> Artificial Sequence


<220> 
<223> vacuole localization domain #5

<400> 33
Val Ser Gly Gly Val Trp Asp Ser Ser Val Glu Thr Asn Ala Thr Ala 
1               5                   10                  15      
Ser Leu Val Ser Glu Met 
            20          

<210> 34
<211> 18
<212> PRT
<213> Artificial Sequence


<220> 
<223> vacuole localization domain #6

<400> 34
Gln Ala His Pro Asn Phe Pro Leu Glu Met Pro Gly Ser Asp Glu Val 
1               5                   10                  15      
Ala Lys 
        

<210> 35
<211> 5
<212> PRT
<213> Artificial Sequence


<220> 
<223> Gly-rich linker #1

<400> 35
Gly Gly Ser Ser Gly 
1               5   

<210> 36
<211> 6
<212> PRT
<213> Artificial Sequence


<220> 
<223> Gly-rich linker #2

<400> 36
Gly Ser Gly Ser Gly Ser 
1               5       

<210> 37
<211> 11
<212> PRT
<213> Artificial Sequence


<220> 
<223> Gly-rich linker #3

<400> 37
Gly Gly Ser Gly Gly Ser Gly Gly Ser Gly Gly 
1               5                   10      

<210> 38
<211> 16
<212> PRT
<213> Artificial Sequence


<220> 
<223> Gly-rich linker #4

<400> 38
Gly Gly Gly Gly Ser Leu Val Pro Arg Gly Ser Gly Gly Gly Gly Ser 
1               5                   10                  15      



<210> 39
<211> 11
<212> PRT
<213> Artificial Sequence


<220> 
<223> Gly-rich linker #5

<400> 39
Gly Gly Ser Gly Gly His Met Gly Ser Gly Gly 
1               5                   10      

<210> 40
<211> 8
<212> PRT
<213> Artificial Sequence


<220> 
<223> Gly-rich linker #6

<400> 40
Ser Gly Gly Gly Ser Ser His Ser 
1               5               

<210> 41
<211> 10
<212> PRT
<213> Artificial Sequence


<220> 
<223> Gly-rich linker #7

<400> 41
Ser Gly Gly Ser Gly Gly Ser Ser His Ser 
1               5                   10  

<210> 42
<211> 13
<212> PRT
<213> Artificial Sequence


<220> 
<223> Gly-rich linker #8

<400> 42
Ser Gly Gly Ser Gly Gly Ser Gly Gly Ser Ser His Ser 
1               5                   10              

<210> 43
<211> 49
<212> DNA
<213> Artificial Sequence


<220> 
<223> primer #1

<400> 43
ttagattagt tcatggtcat agctgtttcc tgttttccca gtcacgacg                 49


<210> 44
<211> 24
<212> DNA
<213> Artificial Sequence


<220> 
<223> primer #2

<400> 44
cctgagcgga taacaatttc acac                                           24


<210> 45
<211> 21
<212> DNA
<213> Artificial Sequence


<220> 
<223> Repeated sequence in the sequence to be circularized from Example
      10

<400> 45
cctgtgtgaa attgttatcc g                                              21


