               SEQUENCE LISTING

<110> PharmACT Holding AG

<120> A MODIFIED BACTERIAL HYALURONIDASE POLYPEPTIDE, PRODUCTION PROCESS, PHARMACEUTICAL COMPOSITIONS AND THEIR USES 

<130> P1035/WO

<160> 8

<170> BiSSAP 1.3.6

<210> 1
<211> 740
<212> PRT
<213> Streptococcus pneumoniae


<220> 
<223> Modified bacterial hyaluronidase D016

<400> 1
Met Gly Trp Ser His Pro Gln Phe Glu Lys Ala Ser Val Lys Asp Thr 
1               5                   10                  15      
Tyr Thr Asp Arg Leu Asp Asp Trp Asn Gly Ile Ile Ala Gly Asn Gln 
            20                  25                  30          
Tyr Tyr Asp Ser Lys Asn Asp Gln Met Ala Lys Leu Asn Gln Glu Leu 
        35                  40                  45              
Glu Gly Lys Val Ala Asp Ser Leu Ser Ser Ile Ser Ser Gln Ala Asp 
    50                  55                  60                  
Arg Ile Tyr Leu Trp Glu Lys Phe Ser Asn Tyr Lys Thr Ser Ala Asn 
65                  70                  75                  80  
Leu Thr Ala Thr Tyr Arg Lys Leu Glu Glu Met Ala Lys Gln Val Thr 
                85                  90                  95      
Asn Pro Ser Ser Arg Tyr Tyr Gln Asp Glu Thr Val Val Arg Thr Val 
            100                 105                 110         
Arg Asp Ser Met Glu Trp Met His Lys His Val Tyr Asn Ser Glu Lys 
        115                 120                 125             
Ser Ile Val Gly Asn Trp Trp Asp Tyr Glu Ile Gly Thr Pro Arg Ala 
    130                 135                 140                 
Ile Asn Asn Thr Leu Ser Leu Met Lys Glu Tyr Phe Ser Asp Glu Glu 
145                 150                 155                 160 
Ile Lys Lys Tyr Thr Asp Val Ile Glu Lys Phe Val Pro Asp Pro Glu 
                165                 170                 175     
His Phe Arg Lys Thr Thr Asp Asn Pro Phe Lys Ala Leu Gly Gly Asn 
            180                 185                 190         
Leu Val Asp Met Gly Arg Val Lys Val Ile Ala Gly Leu Leu Arg Lys 
        195                 200                 205             
Asp Asp Gln Glu Ile Ser Ser Thr Ile Arg Ser Ile Glu Gln Val Phe 
    210                 215                 220                 
Lys Leu Val Asp Gln Gly Glu Gly Phe Tyr Gln Asp Gly Ser Tyr Ile 
225                 230                 235                 240 
Asp His Thr Asn Val Ala Tyr Thr Gly Ala Tyr Gly Asn Val Leu Ile 
                245                 250                 255     
Asp Gly Leu Ser Gln Leu Leu Pro Val Ile Gln Lys Thr Lys Asn Pro 
            260                 265                 270         
Ile Asp Lys Asp Lys Met Gln Thr Met Tyr His Trp Ile Asp Lys Ser 
        275                 280                 285             
Phe Ala Pro Leu Leu Val Asn Gly Glu Leu Met Asp Met Ser Arg Gly 
    290                 295                 300                 
Arg Ser Ile Ser Arg Ala Asn Ser Glu Gly His Val Ala Ala Val Glu 
305                 310                 315                 320 
Val Leu Arg Gly Ile His Arg Ile Ala Asp Met Ser Glu Gly Glu Thr 
                325                 330                 335     
Lys Gln Arg Leu Gln Ser Leu Val Lys Thr Ile Val Gln Ser Asp Ser 
            340                 345                 350         
Tyr Tyr Asp Val Phe Lys Asn Leu Lys Thr Tyr Lys Asp Ile Ser Leu 
        355                 360                 365             
Met Gln Ser Leu Leu Ser Asp Ala Gly Val Ala Ser Val Pro Arg Thr 
    370                 375                 380                 
Ser Tyr Leu Ser Ala Phe Asn Lys Met Asp Lys Thr Ala Met Tyr Asn 
385                 390                 395                 400 
Ala Glu Lys Gly Phe Gly Phe Gly Leu Ser Leu Phe Ser Ser Arg Thr 
                405                 410                 415     
Leu Asn Tyr Glu His Met Asn Lys Glu Asn Lys Arg Gly Trp Tyr Thr 
            420                 425                 430         
Ser Asp Gly Met Phe Tyr Leu Tyr Asn Gly Asp Leu Ser His Tyr Ser 
        435                 440                 445             
Asp Gly Tyr Trp Pro Thr Val Asn Pro Tyr Lys Met Pro Gly Thr Thr 
    450                 455                 460                 
Glu Thr Asp Ala Lys Arg Ala Asp Ser Asp Thr Gly Lys Val Leu Pro 
465                 470                 475                 480 
Ser Ala Phe Val Gly Thr Ser Lys Leu Asp Asp Ala Asn Ala Thr Ala 
                485                 490                 495     
Thr Met Asp Phe Thr Asn Trp Asn Gln Thr Leu Thr Ala His Lys Ser 
            500                 505                 510         
Trp Phe Met Leu Lys Asp Lys Ile Ala Phe Leu Gly Ser Asn Ile Gln 
        515                 520                 525             
Asn Thr Ser Thr Asp Thr Ala Ala Thr Thr Ile Asp Gln Arg Lys Leu 
    530                 535                 540                 
Glu Ser Ser Asn Pro Tyr Lys Val Tyr Val Asn Asp Lys Glu Ala Ser 
545                 550                 555                 560 
Leu Thr Glu Gln Glu Lys Asp Tyr Pro Glu Thr Gln Ser Gly Phe Leu 
                565                 570                 575     
Glu Ser Ser Asp Ser Lys Lys Asn Ile Gly Tyr Phe Phe Phe Lys Lys 
            580                 585                 590         
Ser Ser Ile Ser Met Ser Lys Ala Leu Gln Lys Gly Ala Trp Lys Asp 
        595                 600                 605             
Ile Asn Glu Gly Gln Ser Asp Lys Glu Val Glu Asn Glu Phe Leu Thr 
    610                 615                 620                 
Ile Ser Gln Ala His Lys Gln Asn Gly Asp Ser Tyr Gly Tyr Met Leu 
625                 630                 635                 640 
Ile Pro Asn Val Asp Arg Ala Thr Phe Asn Gln Met Ile Lys Glu Leu 
                645                 650                 655     
Glu Ser Ser Leu Ile Glu Asn Asn Glu Thr Leu Gln Ser Val Tyr Asp 
            660                 665                 670         
Ala Lys Gln Gly Val Trp Gly Ile Val Lys Tyr Asp Asp Ser Val Ser 
        675                 680                 685             
Thr Ile Ser Asn Gln Phe Gln Val Leu Lys Arg Gly Val Tyr Thr Ile 
    690                 695                 700                 
Arg Lys Glu Gly Asp Glu Tyr Lys Ile Ala Tyr Tyr Asn Pro Glu Thr 
705                 710                 715                 720 
Gln Glu Ser Ala Pro Asp Gln Glu Val Phe Lys Lys Leu Glu His His 
                725                 730                 735     
His His His His 
            740 

<210> 2
<211> 2251
<212> DNA
<213> Streptococcus pneumoniae


<220> 
<223> DNA Sequence encoding modified bacterial hyaluronidase D016

<400> 2
ccatgggctg gagccatccg cagtttgaaa aagcaagcgt gaaggatacg tacacggacc      60

gcttagatga ctggaacggg atcatcgcgg ggaaccagta ctatgatagc aaaaacgacc     120

aaatggcaaa attaaatcaa gaattagaag gtaaggtcgc ggattcactc tcaagcatta     180

gcagccaagc tgaccggatc tatttatggg aaaaatttag caactataaa acaagcgcca     240

accttacggc aacgtatcga aaattagagg agatggcaaa gcaggtaacg aacccgagca     300

gccgctatta tcaggatgaa acggtcgtgc ggacggtccg agattcaatg gaatggatgc     360

ataaacatgt ctacaacagc gaaaagtcaa ttgtggggaa ttggtgggat tatgaaatcg     420

gcacgccgcg cgcaatcaat aacacgttaa gccttatgaa agaatacttc agcgatgagg     480

aaattaaaaa atatacggat gtaattgaaa aatttgtccc agatccagaa catttccgga     540

agacaacgga taatccgttc aaggcgctcg gcggtaattt agtggatatg ggtcgagtca     600

aagtcatagc gggcttactt cgcaaggatg atcaggaaat tagcagcacg attcggtcaa     660

ttgagcaggt attcaagtta gtcgaccagg gcgaaggctt ttatcaggat ggttcatata     720

tcgaccacac gaacgtggca tatacaggcg cgtatgggaa cgtgttaatt gatgggttga     780

gccagctttt accggtcatt cagaagacga agaacccgat cgataaagat aaaatgcaga     840

cgatgtacca ctggattgat aaatcatttg cgccgttact tgtaaacggt gagcttatgg     900

atatgagccg cggtcggtca atcagccgcg ccaactcaga ggggcacgta gcagccgtcg     960

aagtcctccg ggggattcac cggatagctg atatgagcga aggtgaaacg aaacagcgct    1020

tacaaagctt agtaaagacg attgtgcagt cagatagcta ttatgatgtc tttaagaact    1080

taaagacgta taaggatatc agcttaatgc agtcattatt aagcgatgcc ggtgtcgcca    1140

gcgtgccgcg gacgagctac ctcagcgcat ttaataagat ggataaaacg gcaatgtaca    1200

acgccgagaa agggtttggt tttgggttaa gcttgttttc aagccgcacg ttaaactacg    1260

aacacatgaa taaggaaaac aaacgcggct ggtatacaag cgatgggatg ttctatttat    1320

acaacgggga tttaagccac tattcagatg ggtactggcc gacggtgaac ccgtataaga    1380

tgccgggcac gacggagaca gatgcgaagc gggctgattc agatacgggc aaagtgttac    1440

ccagcgcgtt cgtgggtaca tcaaaactcg atgatgcaaa cgctacggcc acgatggatt    1500

tcacgaattg gaaccagacg ttaacggcgc ataagtcatg gtttatgctc aaggataaga    1560

ttgcattttt aggttcaaac atccagaata cgagcacgga tacggcggcc acgacgattg    1620

accaacggaa acttgaaagc agcaacccgt ataaagtcta tgtcaacgat aaagaagcat    1680

cattaacgga acaggaaaag gattatccgg aaacgcagag cgggttttta gaatcatcag    1740

attcaaaaaa gaacattggc tactttttct ttaagaagag cagcatcagc atgagcaagg    1800

cgttacagaa gggtgcatgg aaggatatca acgaaggtca aagcgacaag gaagtggaaa    1860

acgaattttt aacaattagc caagcgcata agcagaacgg tgatagctat gggtatatgt    1920

tgattccgaa tgtagatcgc gcaacgttca accagatgat aaaagagtta gaatcatcat    1980

tgatcgaaaa caatgaaacg ttacaaagcg tgtatgatgc aaaacagggt gtgtggggga    2040

ttgtaaaata tgatgatagc gtcagcacga tttcaaatca gttccaggtg ttaaaacgcg    2100

gtgtctatac gattcggaaa gaaggggatg aatataagat tgcatactat aacccggaaa    2160

cgcaagaaag cgcgccggat caagaagtct ttaaaaagct cgagcatcat catcatcatc    2220

actaaccact cgacttcttt tggagctgag c                                   2251


<210> 3
<211> 949
<212> PRT
<213> Streptococcus pneumoniae


<220> 
<223> Wild Type bacterial hyaluronidase sequence

<400> 3
Met Val Pro Ile Glu Ala Lys Lys Lys Tyr Lys Leu Arg Phe Lys Ile 
1               5                   10                  15      
Lys Thr Asp Asn Lys Val Gly Ile Ala Lys Val Arg Ile Ile Glu Glu 
            20                  25                  30          
Ser Gly Lys Asp Lys Arg Leu Trp Asn Ser Ala Thr Thr Ser Gly Thr 
        35                  40                  45              
Lys Asp Trp Gln Thr Ile Glu Ala Asp Tyr Ser Pro Thr Leu Asp Val 
    50                  55                  60                  
Asp Lys Ile Lys Leu Glu Leu Phe Tyr Glu Thr Gly Thr Gly Thr Val 
65                  70                  75                  80  
Ser Phe Lys Asp Ile Glu Leu Val Glu Val Ala Asp Gln Pro Ser Glu 
                85                  90                  95      
Asp Ser Gln Thr Asp Lys Gln Leu Glu Glu Lys Ile Asp Leu Pro Ile 
            100                 105                 110         
Gly Lys Lys His Val Phe Pro Leu Ala Asp Tyr Thr Tyr Lys Val Glu 
        115                 120                 125             
Asn Pro Asp Val Ala Ser Val Lys Asn Gly Ile Leu Glu Pro Leu Lys 
    130                 135                 140                 
Glu Gly Thr Thr Asn Val Ile Val Ser Lys Asp Gly Lys Glu Val Lys 
145                 150                 155                 160 
Lys Ile Pro Leu Lys Ile Leu Ala Ser Val Lys Asp Thr Tyr Thr Asp 
                165                 170                 175     
Arg Leu Asp Asp Trp Asn Gly Ile Ile Ala Gly Asn Gln Tyr Tyr Asp 
            180                 185                 190         
Ser Lys Asn Asp Gln Met Ala Lys Leu Asn Gln Glu Leu Glu Gly Lys 
        195                 200                 205             
Val Ala Asp Ser Leu Ser Ser Ile Ser Ser Gln Ala Asp Arg Ile Tyr 
    210                 215                 220                 
Leu Trp Glu Lys Phe Ser Asn Tyr Lys Thr Ser Ala Asn Leu Thr Ala 
225                 230                 235                 240 
Thr Tyr Arg Lys Leu Glu Glu Met Ala Lys Gln Val Thr Asn Pro Ser 
                245                 250                 255     
Ser Arg Tyr Tyr Gln Asp Glu Thr Val Val Arg Thr Val Arg Asp Ser 
            260                 265                 270         
Met Glu Trp Met His Lys His Val Tyr Asn Ser Glu Lys Ser Ile Val 
        275                 280                 285             
Gly Asn Trp Trp Asp Tyr Glu Ile Gly Thr Pro Arg Ala Ile Asn Asn 
    290                 295                 300                 
Thr Leu Ser Leu Met Lys Glu Tyr Phe Ser Asp Glu Glu Ile Lys Lys 
305                 310                 315                 320 
Tyr Thr Asp Val Ile Glu Lys Phe Val Pro Asp Pro Glu His Phe Arg 
                325                 330                 335     
Lys Thr Thr Asp Asn Pro Phe Lys Ala Leu Gly Gly Asn Leu Val Asp 
            340                 345                 350         
Met Gly Arg Val Lys Val Ile Ala Gly Leu Leu Arg Lys Asp Asp Gln 
        355                 360                 365             
Glu Ile Ser Ser Thr Ile Arg Ser Ile Glu Gln Val Phe Lys Leu Val 
    370                 375                 380                 
Asp Gln Gly Glu Gly Phe Tyr Gln Asp Gly Ser Tyr Ile Asp His Thr 
385                 390                 395                 400 
Asn Val Ala Tyr Thr Gly Ala Tyr Gly Asn Val Leu Ile Asp Gly Leu 
                405                 410                 415     
Ser Gln Leu Leu Pro Val Ile Gln Lys Thr Lys Asn Pro Ile Asp Lys 
            420                 425                 430         
Asp Lys Met Gln Thr Met Tyr His Trp Ile Asp Lys Ser Phe Ala Pro 
        435                 440                 445             
Leu Leu Val Asn Gly Glu Leu Met Asp Met Ser Arg Gly Arg Ser Ile 
    450                 455                 460                 
Ser Arg Ala Asn Ser Glu Gly His Val Ala Ala Val Glu Val Leu Arg 
465                 470                 475                 480 
Gly Ile His Arg Ile Ala Asp Met Ser Glu Gly Glu Thr Lys Gln Arg 
                485                 490                 495     
Leu Gln Ser Leu Val Lys Thr Ile Val Gln Ser Asp Ser Tyr Tyr Asp 
            500                 505                 510         
Val Phe Lys Asn Leu Lys Thr Tyr Lys Asp Ile Ser Leu Met Gln Ser 
        515                 520                 525             
Leu Leu Ser Asp Ala Gly Val Ala Ser Val Pro Arg Thr Ser Tyr Leu 
    530                 535                 540                 
Ser Ala Phe Asn Lys Met Asp Lys Thr Ala Met Tyr Asn Ala Glu Lys 
545                 550                 555                 560 
Gly Phe Gly Phe Gly Leu Ser Leu Phe Ser Ser Arg Thr Leu Asn Tyr 
                565                 570                 575     
Glu His Met Asn Lys Glu Asn Lys Arg Gly Trp Tyr Thr Ser Asp Gly 
            580                 585                 590         
Met Phe Tyr Leu Tyr Asn Gly Asp Leu Ser His Tyr Ser Asp Gly Tyr 
        595                 600                 605             
Trp Pro Thr Val Asn Pro Tyr Lys Met Pro Gly Thr Thr Glu Thr Asp 
    610                 615                 620                 
Ala Lys Arg Ala Asp Ser Asp Thr Gly Lys Val Leu Pro Ser Ala Phe 
625                 630                 635                 640 
Val Gly Thr Ser Lys Leu Asp Asp Ala Asn Ala Thr Ala Thr Met Asp 
                645                 650                 655     
Phe Thr Asn Trp Asn Gln Thr Leu Thr Ala His Lys Ser Trp Phe Met 
            660                 665                 670         
Leu Lys Asp Lys Ile Ala Phe Leu Gly Ser Asn Ile Gln Asn Thr Ser 
        675                 680                 685             
Thr Asp Thr Ala Ala Thr Thr Ile Asp Gln Arg Lys Leu Glu Ser Ser 
    690                 695                 700                 
Asn Pro Tyr Lys Val Tyr Val Asn Asp Lys Glu Ala Ser Leu Thr Glu 
705                 710                 715                 720 
Gln Glu Lys Asp Tyr Pro Glu Thr Gln Ser Gly Phe Leu Glu Ser Ser 
                725                 730                 735     
Asp Ser Lys Lys Asn Ile Gly Tyr Phe Phe Phe Lys Lys Ser Ser Ile 
            740                 745                 750         
Ser Met Ser Lys Ala Leu Gln Lys Gly Ala Trp Lys Asp Ile Asn Glu 
        755                 760                 765             
Gly Gln Ser Asp Lys Glu Val Glu Asn Glu Phe Leu Thr Ile Ser Gln 
    770                 775                 780                 
Ala His Lys Gln Asn Gly Asp Ser Tyr Gly Tyr Met Leu Ile Pro Asn 
785                 790                 795                 800 
Val Asp Arg Ala Thr Phe Asn Gln Met Ile Lys Glu Leu Glu Ser Ser 
                805                 810                 815     
Leu Ile Glu Asn Asn Glu Thr Leu Gln Ser Val Tyr Asp Ala Lys Gln 
            820                 825                 830         
Gly Val Trp Gly Ile Val Lys Tyr Asp Asp Ser Val Ser Thr Ile Ser 
        835                 840                 845             
Asn Gln Phe Gln Val Leu Lys Arg Gly Val Tyr Thr Ile Arg Lys Glu 
    850                 855                 860                 
Gly Asp Glu Tyr Lys Ile Ala Tyr Tyr Asn Pro Glu Thr Gln Glu Ser 
865                 870                 875                 880 
Ala Pro Asp Gln Glu Val Phe Lys Lys Leu Glu Gln Ala Ala Gln Pro 
                885                 890                 895     
Gln Val Gln Asn Ser Lys Glu Lys Glu Lys Ser Glu Glu Glu Lys Asn 
            900                 905                 910         
His Ser Asp Gln Lys Asn Leu Pro Gln Thr Gly Glu Gly Gln Ser Ile 
        915                 920                 925             
Leu Ala Ser Leu Gly Phe Leu Leu Leu Gly Ala Phe Tyr Leu Phe Arg 
    930                 935                 940                 
Arg Gly Lys Asn Asn 
945                 

<210> 4
<211> 2850
<212> DNA
<213> Streptococcus pneumoniae


<220> 
<223> DNA Sequence encoding Wilde Type bacterial hyaluronidase sequence

<400> 4
atggttccta ttgaagctaa gaaaaagtat aaactgcgtt tcaagattaa aacagataat      60

aaagtcggga ttgccaaagt tcgtatcatt gaggaaagtg gtaaggacaa gcgattgtgg     120

aattctgcaa cgacgtcagg aacaaaggac tggcagacca ttgaagcaga ctatagcccg     180

actttagatg ttgataaaat caagctggag ttattctatg aaacaggaac tgggactgtt     240

tcctttaagg atattgagct ggtagaggta gcagaccagc cttctgagga ttctcaaaca     300

gataaacaac ttgaggaaaa gattgattta ccaattggaa aaaaacatgt ttttcctctt     360

gcggactata cttataaggt agaaaatcct gacgttgctt cagtcaaaaa tggaatttta     420

gaacctctta aggaagggac aaccaatgtc attgtcagta aagatggcaa ggaagtgaaa     480

aagattcctt tgaagattct agcctctgtt aaggatacat acacagaccg tttggatgac     540

tggaatggca tcatcgctgg gaatcaatac tatgattcta aaaatgacca gatggccaaa     600

ttaaaccagg aattggaagg aaaggtagct gatagcctat ccagtatttc aagtcaggcg     660

gaccgcatct atttgtggga aaaattttca aattataaaa cgtctgcaaa tctgactgcc     720

acttatcgga aattggagga gatggccaag caagtgacca atccttcttc tcgttattat     780

caagatgaaa ctgtcgttcg aacagtcagg gattccatgg aatggatgca taaacatgtc     840

tacaatagtg aaaagagcat tgttgggaac tggtgggatt atgaaatcgg tacacctcgt     900

gccatcaaca ataccttgtc tctgatgaaa gaatacttct ctgatgagga aattaaaaaa     960

tatacagatg tgattgaaaa atttgtacca gatcccgaac atttccgaaa gacgactgat    1020

aacccattca aggctctagg tggaaactta gttgatatgg gaagggtaaa agtaatagct    1080

ggtttactgc gtaaggatga tcaagaaatt tcttctacca ttcgctcgat tgagcaagtg    1140

ttcaagttgg tagaccaagg tgaaggtttt tatcaagatg gatcctatat cgaccacacc    1200

aatgttgcct atacgggtgc ttatgggaat gttttgattg atggcctgtc tcaactgttg    1260

ccagtcattc aaaagaccaa gaatccaatc gataaagata aaatgcaaac catgtaccac    1320

tggattgata aatcgtttgc tcctttgctg gtgaatggag agctgatgga tatgagtcgt    1380

ggacgctcga tcagtcgtgc aaatagcgag gggcacgtgg ccgcagtaga agtactaaga    1440

gggattcacc gaatagcgga tatgtctgaa ggagaaacca aacaacgttt gcagagtctt    1500

gtgaagacca ttgttcaatc ggatagttat tatgatgtct ttaagaattt gaagacttat    1560

aaggatatca gtttgatgca atccttgtta agtgatgcag gagtcgcaag tgttccaaga    1620

acaagttacc tatctgcctt taacaagatg gataaaacag ccatgtacaa tgcagagaaa    1680

gggtttggat ttggcttgtc actcttttcc agtcgtacct tgaattacga acacatgaac    1740

aaggaaaata aacgtggttg gtatacgagt gatgggatgt tctatcttta caatggcgat    1800

ttgagtcact atagcgatgg ctactggcca acagttaatc catataagat gcctggtaca    1860

acagagacgg atgctaagag agcggatagc gatacaggta aagttttacc gtctgctttc    1920

gttggaacga gcaaactaga tgatgccaat gcgacagcaa ccatggattt caccaactgg    1980

aatcaaacat tgactgctca taagagctgg tttatgctaa aggataagat tgccttttta    2040

ggaagcaata tccaaaacac ttcaacagat actgctgcaa ctacaattga ccagagaaaa    2100

ctggaatcaa gtaatccata taaagtctat gtcaatgata aagaagcctc ccttacagaa    2160

caagaaaagg attatcctga aacccaaagt gggtttttag aatcgtccga ttcgaaaaag    2220

aatattggtt actttttctt taagaagagt tcaatcagta tgagtaaggc tttgcaaaag    2280

ggagcctgga aggatatcaa tgaaggacag tcagacaagg aagttgaaaa tgaatttctt    2340

acgattagtc aggctcataa gcaaaatgga gattcttatg gctatatgct cattcctaac    2400

gtggatcgtg ccaccttcaa tcaaatgata aaagagttag aaagcagcct catcgaaaat    2460

aacgaaaccc ttcagtctgt ttatgatgcc aaacaaggag tttggggcat tgtgaaatat    2520

gatgattctg tctctactat ttccaaccaa ttccaagttt tgaaacgtgg agtctatact    2580

attcgaaaag aaggggatga atataagatt gcctactata atcctgaaac ccaggaatca    2640

gctccagatc aggaagtctt taaaaagcta gagcaagcag ctcagccaca agtacagaat    2700

tcaaaagaaa aggaaaaatc tgaagaggaa aagaaccatt cggatcaaaa gaatctccct    2760

cagacaggag aaggtcagtc aatcttggca agtctagggt tcttgctact tggggcgttt    2820

tatttattcc gtagaggaaa gaacaactaa                                     2850


<210> 5
<211> 8
<212> PRT
<213> Artificial Sequence


<220> 
<223> Strep Tag Amino Acid Sequence

<400> 5
Trp Ser His Pro Gln Phe Glu Lys 
1               5               

<210> 6
<211> 24
<212> DNA
<213> Artificial Sequence


<220> 
<223> DNA Sequence encoding Strep Tag Amino Acid Sequence

<400> 6
tggagccatc cgcagtttga aaaa                                           24


<210> 7
<211> 6
<212> PRT
<213> Artificial Sequence


<220> 
<223> His Tag Amino Acid Sequence

<400> 7
His His His His His His 
1               5       

<210> 8
<211> 18
<212> DNA
<213> Artificial Sequence


<220> 
<223> DNA Sequence encoding His Tag Amino Acid Sequence

<400> 8
catcatcatc atcatcac                                                  18


