б

<110>  ѧҽԺѧٴҽѧԺƼ޹˾

<120>  RHO-adRPڻ༭ķ

<130>  0138-PA-010

<160>  61

<170>  PatentIn version 3.5

<210>  1
<211>  21
<212>  DNA
<213>  ˹УArtificial Sequence

<220>
<223>  RHO17-SgRNA1

<400>  1
cgtaccacac ccatcgcatt g                                                 21


<210>  2
<211>  21
<212>  DNA
<213>  ˹УArtificial Sequence

<220>
<223>  RHO17-SgRNA2

<400>  2
tgcgtaccac acccatcgca t                                                 21


<210>  3
<211>  21
<212>  DNA
<213>  ˹УArtificial Sequence

<220>
<223>  RHO17-SgRNA3

<400>  3
ctgcgtacca cacccatcgc a                                                 21


<210>  4
<211>  21
<212>  DNA
<213>  ˹УArtificial Sequence

<220>
<223>  RHO135-SgRNA1

<400>  4
caccacgtac cactcgatgg c                                                 21


<210>  5
<211>  21
<212>  DNA
<213>  ˹УArtificial Sequence

<220>
<223>  RHO135-SgRNA2

<400>  5
cactcgatgg ccaggaccac c                                                 21


<210>  6
<211>  60
<212>  DNA
<213>  ˹УArtificial Sequence

<220>
<223>  RHO17 SgRNA1-T7

<400>  6
taatacgact cactatagcg taccacaccc atcgcattgg ttttagtact ctggaaacag       60


<210>  7
<211>  60
<212>  DNA
<213>  ˹УArtificial Sequence

<220>
<223>  RHO17 SgRNA2-T7

<400>  7
taatacgact cactatagtg cgtaccacac ccatcgcatg ttttagtact ctggaaacag       60


<210>  8
<211>  60
<212>  DNA
<213>  ˹УArtificial Sequence

<220>
<223>  RHO17 SgRNA3-T7 

<400>  8
taatacgact cactatagct gcgtaccaca cccatcgcag ttttagtact ctggaaacag       60


<210>  9
<211>  60
<212>  DNA
<213>  ˹УArtificial Sequence

<220>
<223>  RHO135 SgRNA1-T7

<400>  9
taatacgact cactatagca ccacgtacca ctcgatggcg ttttagtact ctggaaacag       60


<210>  10
<211>  60
<212>  DNA
<213>  ˹УArtificial Sequence

<220>
<223>  RHO135 SgRNA2-T7

<400>  10
taatacgact cactatagca ctcgatggcc aggaccaccg ttttagtact ctggaaacag       60


<210>  11
<211>  22
<212>  DNA
<213>  ˹УArtificial Sequence

<220>
<223>  

<400>  11
atctcgccaa caagttgacg ag                                                22


<210>  12
<211>  23
<212>  DNA
<213>  ˹УArtificial Sequence

<220>
<223>  RHO17-1

<400>  12
ttaggagggg gaggtcactt tat                                               23


<210>  13
<211>  22
<212>  DNA
<213>  ˹УArtificial Sequence

<220>
<223>  RHO17-1

<400>  13
aaatccactt cccaccctga gc                                                22


<210>  14
<211>  21
<212>  DNA
<213>  ˹УArtificial Sequence

<220>
<223>  RHO135-1

<400>  14
gcccagagcg ctaagcaaat a                                                 21


<210>  15
<211>  20
<212>  DNA
<213>  ˹УArtificial Sequence

<220>
<223>  RHO135-1

<400>  15
atcgcctaga ggctgagtgg                                                   20


<210>  16
<211>  30
<212>  DNA
<213>  ˹УArtificial Sequence

<220>
<223>  RHO17-mKate-mut-sgRNA1 

<400>  16
ctagcgtacc acacccatcg cattggagaa                                        30


<210>  17
<211>  30
<212>  DNA
<213>  ˹УArtificial Sequence

<220>
<223>  RHO17-mKate-mut-sgRNA1 

<400>  17
ggccttctcc aatgcgatgg gtgtggtacg                                        30


<210>  18
<211>  30
<212>  DNA
<213>  ˹УArtificial Sequence

<220>
<223>  RHO17-mKate-wt-sgRNA1 

<400>  18
ctagcgtacc acacccgtcg cattggagaa                                        30


<210>  19
<211>  30
<212>  DNA
<213>  ˹УArtificial Sequence

<220>
<223>  RHO17-mKate-wt-sgRNA1 

<400>  19
ggccttctcc aatgcgacgg gtgtggtacg                                        30


<210>  20
<211>  30
<212>  DNA
<213>  ˹УArtificial Sequence

<220>
<223>  RHO17-mKate-mut-sgRNA2 

<400>  20
ctagtgcgta ccacacccat cgcattggag                                        30


<210>  21
<211>  30
<212>  DNA
<213>  ˹УArtificial Sequence

<220>
<223>  RHO17-mKate-mut-sgRNA2 

<400>  21
ggccctccaa tgcgatgggt gtggtacgca                                        30


<210>  22
<211>  30
<212>  DNA
<213>  ˹УArtificial Sequence

<220>
<223>  RHO17-mKate-wt-sgRNA2 

<400>  22
ctagtgcgta ccacacccgt cgcattggag                                        30


<210>  23
<211>  30
<212>  DNA
<213>  ˹УArtificial Sequence

<220>
<223>  RHO17-mKate-wt-sgRNA2 

<400>  23
ggccctccaa tgcgacgggt gtggtacgca                                        30


<210>  24
<211>  30
<212>  DNA
<213>  ˹УArtificial Sequence

<220>
<223>  RHO17-mKate-mut-sgRNA3 

<400>  24
ctagctgcgt accacaccca tcgcattgga                                        30


<210>  25
<211>  30
<212>  DNA
<213>  ˹УArtificial Sequence

<220>
<223>  RHO17-mKate-mut-sgRNA3 

<400>  25
ggcctccaat gcgatgggtg tggtacgcag                                        30


<210>  26
<211>  30
<212>  DNA
<213>  ˹УArtificial Sequence

<220>
<223>  RHO17-mKate-wt-sgRNA3 

<400>  26
ctagctgcgt accacacccg tcgcattgga                                        30


<210>  27
<211>  30
<212>  DNA
<213>  ˹УArtificial Sequence

<220>
<223>  RHO17-mKate-wt-sgRNA3 

<400>  27
ggcctccaat gcgacgggtg tggtacgcag                                        30


<210>  28
<211>  30
<212>  DNA
<213>  ˹УArtificial Sequence

<220>
<223>  RHO135-mKate-mut-sgRNA1 

<400>  28
ctagcaccac gtaccactcg atggccagga                                        30


<210>  29
<211>  30
<212>  DNA
<213>  ˹УArtificial Sequence

<220>
<223>  RHO135-mKate-mut-sgRNA1 

<400>  29
ggcctcctgg ccatcgagtg gtacgtggtg                                        30


<210>  30
<211>  30
<212>  DNA
<213>  ˹УArtificial Sequence

<220>
<223>  RHO135-mKate-wt-sgRNA1 

<400>  30
ctagcaccac gtaccgctcg atggccagga                                        30


<210>  31
<211>  30
<212>  DNA
<213>  ˹УArtificial Sequence

<220>
<223>  RHO135-mKate-wt-sgRNA1 

<400>  31
ggcctcctgg ccatcgagcg gtacgtggtg                                        30


<210>  32
<211>  30
<212>  DNA
<213>  ˹УArtificial Sequence

<220>
<223>  RHO135-mKate-mut-sgRNA2 

<400>  32
ctagcactcg atggccagga ccaccaagga                                        30


<210>  33
<211>  30
<212>  DNA
<213>  ˹УArtificial Sequence

<220>
<223>  RHO135-mKate-mut-sgRNA2 

<400>  33
ggcctccttg gtggtcctgg ccatcgagtg                                        30


<210>  34
<211>  30
<212>  DNA
<213>  ˹УArtificial Sequence

<220>
<223>  RHO135-mKate-wt-sgRNA2 

<400>  34
ctagcgctcg atggccagga ccaccaagga                                        30


<210>  35
<211>  30
<212>  DNA
<213>  ˹УArtificial Sequence

<220>
<223>  RHO135-mKate-wt-sgRNA2 

<400>  35
ggcctccttg gtggtcctgg ccatcgagcg                                        30


<210>  36
<211>  21
<212>  DNA
<213>  ˹УArtificial Sequence

<220>
<223>  TS-SP001

<400>  36
ctgataggca gcctgcacct g                                                 21


<210>  37
<211>  317
<212>  DNA
<213>  ˹УArtificial Sequence

<220>
<223>  I

<400>  37
ctggctacca gcttcatgta cggcagcaaa accttcatca accacaccca gggcatcccc       60

gacttcttta agcagtcctt ccctgagggc ttcacatggg agagagtcac cacatacgaa      120

gacgggggcg tgctgaccgc tacccaggac accagcctcc aggacggctg cctcatctac      180

aacgtcaaga tcagaggggt gaacttccca tccaacggcc ctgtgatgca gaagaaaaca      240

ctcggctggg aggcctccac cgagatgctg taccccgctg acggcggcct ggaaggcaga      300

agcgacatgt aatctag                                                     317


<210>  38
<211>  605
<212>  DNA
<213>  ˹УArtificial Sequence

<220>
<223>  II

<400>  38
ccgctggcta ccagcttcat gtacggcagc aaaaccttca tcaaccacac ccagggcatc       60

cccgacttct ttaagcagtc cttccctgag ggcttcacat gggagagagt caccacatac      120

gaagacgggg gcgtgctgac cgctacccag gacaccagcc tccaggacgg ctgcctcatc      180

tacaacgtca agatcagagg ggtgaacttc ccatccaacg gccctgtgat gcagaagaaa      240

acactcggct gggaggcctc caccgagatg ctgtaccccg ctgacggcgg cctggaaggc      300

agaagcgaca tggccctgaa gctcgtgggc gggggccacc tgatctgcaa cttgaagacc      360

acatacagat ccaagaaacc cgctaagaac ctcaagatgc ccggcgtcta ctatgtggac      420

agaagactgg aaagaatcaa ggaggccgac aaagagacct acgtcgagca gcacgaggtg      480

gctgtggcca gatactgcga cctccctagc aaactggggc acaaacttaa ttgacccggg      540

tacccagctt tcttgtacaa agtggtacgc gtgaattcac tcctcaggtg caggctgcct      600

atcag                                                                  605


<210>  39
<211>  5
<212>  DNA
<213>  ˹УArtificial Sequence

<220>
<223>  PAM1

<400>  39
gagaa                                                                    5


<210>  40
<211>  5
<212>  DNA
<213>  ˹УArtificial Sequence

<220>
<223>  PAM2

<400>  40
tggag                                                                    5


<210>  41
<211>  5
<212>  DNA
<213>  ˹УArtificial Sequence

<220>
<223>  PAM3

<400>  41
ttgga                                                                    5


<210>  42
<211>  5
<212>  DNA
<213>  ˹УArtificial Sequence

<220>
<223>  PAM4

<400>  42
cagga                                                                    5


<210>  43
<211>  5
<212>  DNA
<213>  ˹УArtificial Sequence

<220>
<223>  PAM5

<400>  43
aagga                                                                    5


<210>  44
<211>  21
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  RHO17-SgRNA1

<400>  44
cguaccacac ccaucgcauu g                                                 21


<210>  45
<211>  21
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  RHO17-SgRNA2

<400>  45
ugcguaccac acccaucgca u                                                 21


<210>  46
<211>  21
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  RHO17-SgRNA3

<400>  46
cugcguacca cacccaucgc a                                                 21


<210>  47
<211>  21
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  RHO135-SgRNA1

<400>  47
caccacguac cacucgaugg c                                                 21


<210>  48
<211>  21
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  RHO135-SgRNA2

<400>  48
cacucgaugg ccaggaccac c                                                 21


<210>  49
<211>  22
<212>  DNA
<213>  ˹УArtificial Sequence

<220>
<223>  RHO17-2

<400>  49
ttatgaacac ccccaatctc cc                                                22


<210>  50
<211>  22
<212>  DNA
<213>  ˹УArtificial Sequence

<220>
<223>  RHO17-2

<400>  50
gagggctttg gataacattg ac                                                22


<210>  51
<211>  21
<212>  DNA
<213>  ˹УArtificial Sequence

<220>
<223>  RHO17-3

<400>  51
ctagtgtcac cttggcccct c                                                 21


<210>  52
<211>  21
<212>  DNA
<213>  ˹УArtificial Sequence

<220>
<223>  RHO17-3

<400>  52
tgctgcaaac atggcccgag a                                                 21


<210>  53
<211>  20
<212>  DNA
<213>  ˹УArtificial Sequence

<220>
<223>  RHO135-2

<400>  53
tggctcctag gagaggcccc                                                   20


<210>  54
<211>  20
<212>  DNA
<213>  ˹УArtificial Sequence

<220>
<223>  RHO135-2

<400>  54
ctgtttcttc ttctgcccta                                                   20


<210>  55
<211>  25
<212>  DNA
<213>  ˹УArtificial Sequence

<220>
<223>  WT-F

<400>  55
ggcagcagtg ggattagcgt tagta                                             25


<210>  56
<211>  25
<212>  DNA
<213>  ˹УArtificial Sequence

<220>
<223>  WT-R

<400>  56
tgtgtagagg gtggtggtga atcct                                             25


<210>  57
<211>  25
<212>  DNA
<213>  ˹УArtificial Sequence

<220>
<223>  Mut-R

<400>  57
acgatcagca gaaacatgta ggcgg                                             25


<210>  58
<211>  20
<212>  DNA
<213>  ˹УArtificial Sequence

<220>
<223>  RHO17-4

<400>  58
gagtgtgggg actggatgac                                                   20


<210>  59
<211>  20
<212>  DNA
<213>  ˹УArtificial Sequence

<220>
<223>  RHO17-4

<400>  59
gggactctcc cagacccctc                                                   20


<210>  60
<211>  22
<212>  DNA
<213>  ˹УArtificial Sequence

<220>
<223>  RHO135-3

<400>  60
tgtccgggtt atttcatttc cc                                                22


<210>  61
<211>  20
<212>  DNA
<213>  ˹УArtificial Sequence

<220>
<223>  RHO135-3

<400>  61
gagatgggac cagcccttgt                                                   20

