                         SEQUENCE LISTING

<110>  Medicago Inc.
       Hendin, Hilary E.
       Landry, Nathalie
       Ward, Brian J.
 
<120>  Suprastructure comprising modified influenza hemagglutinin with 
       reduced interaction with sialic acid

<130>  V814907WO

<150>  US 63/014,008
<151>  2020-04-22

<160>  209   

<170>  PatentIn version 3.5

<210>  1
<211>  1722
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PDI-H1A/California/7/2009

<400>  1
atggcgaaaa acgttgcgat tttcggctta ttgttttctc ttcttgtgtt ggttccttct       60

cagatcttcg ctgacacatt atgtataggt tatcatgcga acaattcaac agacactgta      120

gacacagtac tagaaaagaa tgtaacagta acacactctg ttaaccttct agaagacaag      180

cataacggga aactatgcaa actaagaggg gtagccccat tgcatttggg taaatgtaac      240

attgctggct ggatcctggg aaatccagag tgtgaatcac tctccacagc aagctcatgg      300

tcctacattg tggaaacacc tagttcagac aatggaacgt gttacccagg agatttcatc      360

gattatgagg agctaagaga gcaattgagc tcagtgtcat catttgaaag gtttgagata      420

ttccccaaga caagttcatg gcccaatcat gactcgaaca aaggtgtaac ggcagcatgt      480

cctcatgctg gagcaaaaag cttctacaaa aatttaatat ggctagttaa aaaaggaaat      540

tcatacccaa agctcagcaa atcctacatt aatgataaag ggaaagaagt cctcgtgcta      600

tggggcattc accatccatc tactagtgct gaccaacaaa gtctctatca gaatgcagat      660

gcatatgttt ttgtggggtc atcaagatac agcaagaagt tcaagccgga aatagcaata      720

agacccaaag tgagggatca agaagggaga atgaactatt actggacact agtagagccg      780

ggagacaaaa taacattcga agcaactgga aatctagtgg taccgagata tgcattcgca      840

atggaaagaa atgctggatc tggtattatc atttcagata caccagtcca cgattgcaat      900

acaacttgtc aaacacccaa gggtgctata aacaccagcc tcccatttca gaatatacat      960

ccgatcacaa ttggaaaatg tccaaaatat gtaaaaagca caaaattgag actggccaca     1020

ggattgagga atatcccgtc tattcaatct agaggactat ttggggccat tgccggtttc     1080

attgaagggg ggtggacagg gatggtagat ggatggtacg gttatcacca tcaaaatgag     1140

caggggtcag gatatgcagc cgacctgaag agcacacaga atgccattga cgagattact     1200

aacaaagtaa attctgttat tgaaaagatg aatacacagt tcacagcagt aggtaaagag     1260

ttcaaccacc tggaaaaaag aatagagaat ttaaataaaa aagttgatga tggtttcctg     1320

gacatttgga cttacaatgc cgaactgttg gttctattgg aaaatgaaag aactttggac     1380

taccacgatt caaatgtgaa gaacttatat gaaaaggtaa gaagccagct aaaaaacaat     1440

gccaaggaaa ttggaaacgg ctgctttgaa ttttaccaca aatgcgataa cacgtgcatg     1500

gaaagtgtca aaaatgggac ttatgactac ccaaaatact cagaggaagc aaaattaaac     1560

agagaagaaa tagatggggt aaagctggaa tcaacaagga tttaccagat tttggcgatc     1620

tattcaactg tcgccagttc attggtactg gtagtctccc tgggggcaat cagtttctgg     1680

atgtgctcta atgggtctct acagtgtaga atatgtattt aa                        1722


<210>  2
<211>  573
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  PDI-H1 A/ California/7/2009

<400>  2

Met Ala Lys Asn Val Ala Ile Phe Gly Leu Leu Phe Ser Leu Leu Val 
1               5                   10                  15      


Leu Val Pro Ser Gln Ile Phe Ala Asp Thr Leu Cys Ile Gly Tyr His 
            20                  25                  30          


Ala Asn Asn Ser Thr Asp Thr Val Asp Thr Val Leu Glu Lys Asn Val 
        35                  40                  45              


Thr Val Thr His Ser Val Asn Leu Leu Glu Asp Lys His Asn Gly Lys 
    50                  55                  60                  


Leu Cys Lys Leu Arg Gly Val Ala Pro Leu His Leu Gly Lys Cys Asn 
65                  70                  75                  80  


Ile Ala Gly Trp Ile Leu Gly Asn Pro Glu Cys Glu Ser Leu Ser Thr 
                85                  90                  95      


Ala Ser Ser Trp Ser Tyr Ile Val Glu Thr Pro Ser Ser Asp Asn Gly 
            100                 105                 110         


Thr Cys Tyr Pro Gly Asp Phe Ile Asp Tyr Glu Glu Leu Arg Glu Gln 
        115                 120                 125             


Leu Ser Ser Val Ser Ser Phe Glu Arg Phe Glu Ile Phe Pro Lys Thr 
    130                 135                 140                 


Ser Ser Trp Pro Asn His Asp Ser Asn Lys Gly Val Thr Ala Ala Cys 
145                 150                 155                 160 


Pro His Ala Gly Ala Lys Ser Phe Tyr Lys Asn Leu Ile Trp Leu Val 
                165                 170                 175     


Lys Lys Gly Asn Ser Tyr Pro Lys Leu Ser Lys Ser Tyr Ile Asn Asp 
            180                 185                 190         


Lys Gly Lys Glu Val Leu Val Leu Trp Gly Ile His His Pro Ser Thr 
        195                 200                 205             


Ser Ala Asp Gln Gln Ser Leu Tyr Gln Asn Ala Asp Ala Tyr Val Phe 
    210                 215                 220                 


Val Gly Ser Ser Arg Tyr Ser Lys Lys Phe Lys Pro Glu Ile Ala Ile 
225                 230                 235                 240 


Arg Pro Lys Val Arg Asp Gln Glu Gly Arg Met Asn Tyr Tyr Trp Thr 
                245                 250                 255     


Leu Val Glu Pro Gly Asp Lys Ile Thr Phe Glu Ala Thr Gly Asn Leu 
            260                 265                 270         


Val Val Pro Arg Tyr Ala Phe Ala Met Glu Arg Asn Ala Gly Ser Gly 
        275                 280                 285             


Ile Ile Ile Ser Asp Thr Pro Val His Asp Cys Asn Thr Thr Cys Gln 
    290                 295                 300                 


Thr Pro Lys Gly Ala Ile Asn Thr Ser Leu Pro Phe Gln Asn Ile His 
305                 310                 315                 320 


Pro Ile Thr Ile Gly Lys Cys Pro Lys Tyr Val Lys Ser Thr Lys Leu 
                325                 330                 335     


Arg Leu Ala Thr Gly Leu Arg Asn Ile Pro Ser Ile Gln Ser Arg Gly 
            340                 345                 350         


Leu Phe Gly Ala Ile Ala Gly Phe Ile Glu Gly Gly Trp Thr Gly Met 
        355                 360                 365             


Val Asp Gly Trp Tyr Gly Tyr His His Gln Asn Glu Gln Gly Ser Gly 
    370                 375                 380                 


Tyr Ala Ala Asp Leu Lys Ser Thr Gln Asn Ala Ile Asp Glu Ile Thr 
385                 390                 395                 400 


Asn Lys Val Asn Ser Val Ile Glu Lys Met Asn Thr Gln Phe Thr Ala 
                405                 410                 415     


Val Gly Lys Glu Phe Asn His Leu Glu Lys Arg Ile Glu Asn Leu Asn 
            420                 425                 430         


Lys Lys Val Asp Asp Gly Phe Leu Asp Ile Trp Thr Tyr Asn Ala Glu 
        435                 440                 445             


Leu Leu Val Leu Leu Glu Asn Glu Arg Thr Leu Asp Tyr His Asp Ser 
    450                 455                 460                 


Asn Val Lys Asn Leu Tyr Glu Lys Val Arg Ser Gln Leu Lys Asn Asn 
465                 470                 475                 480 


Ala Lys Glu Ile Gly Asn Gly Cys Phe Glu Phe Tyr His Lys Cys Asp 
                485                 490                 495     


Asn Thr Cys Met Glu Ser Val Lys Asn Gly Thr Tyr Asp Tyr Pro Lys 
            500                 505                 510         


Tyr Ser Glu Glu Ala Lys Leu Asn Arg Glu Glu Ile Asp Gly Val Lys 
        515                 520                 525             


Leu Glu Ser Thr Arg Ile Tyr Gln Ile Leu Ala Ile Tyr Ser Thr Val 
    530                 535                 540                 


Ala Ser Ser Leu Val Leu Val Val Ser Leu Gly Ala Ile Ser Phe Trp 
545                 550                 555                 560 


Met Cys Ser Asn Gly Ser Leu Gln Cys Arg Ile Cys Ile 
                565                 570             


<210>  3
<211>  49
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer IF-CPMV(fl5'UTR)_SpPDI.c

<400>  3
tcgtgcttcg gcaccagtac aatggcgaaa aacgttgcga ttttcggct                   49


<210>  4
<211>  46
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer IF-H1cTMCT.S1-4r

<400>  4
actaaagaaa ataggccttt aaatacatat tctacactgt agagac                      46


<210>  5
<211>  4540
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Cloning vector 1190 from left to right T-DNA

<400>  5
tggcaggata tattgtggtg taaacaaatt gacgcttaga caacttaata acacattgcg       60

gacgttttta atgtactgaa ttaacgccga atcccgggct ggtatattta tatgttgtca      120

aataactcaa aaaccataaa agtttaagtt agcaagtgtg tacattttta cttgaacaaa      180

aatattcacc tactactgtt ataaatcatt attaaacatt agagtaaaga aatatggatg      240

ataagaacaa gagtagtgat attttgacaa caattttgtt gcaacatttg agaaaatttt      300

gttgttctct cttttcattg gtcaaaaaca atagagagag aaaaaggaag agggagaata      360

aaaacataat gtgagtatga gagagaaagt tgtacaaaag ttgtaccaaa atagttgtac      420

aaatatcatt gaggaatttg acaaaagcta cacaaataag ggttaattgc tgtaaataaa      480

taaggatgac gcattagaga gatgtaccat tagagaattt ttggcaagtc attaaaaaga      540

aagaataaat tatttttaaa attaaaagtt gagtcatttg attaaacatg tgattattta      600

atgaattgat gaaagagttg gattaaagtt gtattagtaa ttagaatttg gtgtcaaatt      660

taatttgaca tttgatcttt tcctatatat tgccccatag agtcagttaa ctcattttta      720

tatttcatag atcaaataag agaaataacg gtatattaat ccctccaaaa aaaaaaaacg      780

gtatatttac taaaaaatct aagccacgta ggaggataac aggatccccg taggaggata      840

acatccaatc caaccaatca caacaatcct gatgagataa cccactttaa gcccacgcat      900

ctgtggcaca tctacattat ctaaatcaca cattcttcca cacatctgag ccacacaaaa      960

accaatccac atctttatca cccattctat aaaaaatcac actttgtgag tctacacttt     1020

gattcccttc aaacacatac aaagagaaga gactaattaa ttaattaatc atcttgagag     1080

aaaatggaac gagctataca aggaaacgac gctagggaac aagctaacag tgaacgttgg     1140

gatggaggat caggaggtac cacttctccc ttcaaacttc ctgacgaaag tccgagttgg     1200

actgagtggc ggctacataa cgatgagacg aattcgaatc aagataatcc ccttggtttc     1260

aaggaaagct ggggtttcgg gaaagttgta tttaagagat atctcagata cgacaggacg     1320

gaagcttcac tgcacagagt ccttggatct tggacgggag attcggttaa ctatgcagca     1380

tctcgatttt tcggtttcga ccagatcgga tgtacctata gtattcggtt tcgaggagtt     1440

agtatcaccg tttctggagg gtcgcgaact cttcagcatc tctgtgagat ggcaattcgg     1500

tctaagcaag aactgctaca gcttgcccca atcgaagtgg aaagtaatgt atcaagagga     1560

tgccctgaag gtactcaaac cttcgaaaaa gaaagcgagt aagttaaaat gcttcttcgt     1620

ctcctattta taatatggtt tgttattgtt aattttgttc ttgtagaaga gcttaattaa     1680

tcgttgttgt tatgaaatac tatttgtatg agatgaactg gtgtaatgta attcatttac     1740

ataagtggag tcagaatcag aatgtttcct ccataactaa ctagacatga agacctgccg     1800

cgtacaattg tcttatattt gaacaactaa aattgaacat cttttgccac aactttataa     1860

gtggttaata tagctcaaat atatggtcaa gttcaataga ttaataatgg aaatatcagt     1920

tatcgaaatt cattaacaat caacttaacg ttattaacta ctaattttat atcatcccct     1980

ttgataaatg atagtacacc aattaggaag gagcatgctc gcctaggaga ttgtcgtttc     2040

ccgccttcag tttgcaagct gctctagccg tgtagccaat acgcaaaccg cctctccccg     2100

cgcgttggga attactagcg cgtgtcgaca agcttgcatg ccggtcaaca tggtggagca     2160

cgacacactt gtctactcca aaaatatcaa agatacagtc tcagaagacc aaagggcaat     2220

tgagactttt caacaaaggg taatatccgg aaacctcctc ggattccatt gcccagctat     2280

ctgtcacttt attgtgaaga tagtggaaaa ggaaggtggc tcctacaaat gccatcattg     2340

cgataaagga aaggccatcg ttgaagatgc ctctgccgac agtggtccca aagatggacc     2400

cccacccacg aggagcatcg tggaaaaaga agacgttcca accacgtctt caaagcaagt     2460

ggattgatgt gataacatgg tggagcacga cacacttgtc tactccaaaa atatcaaaga     2520

tacagtctca gaagaccaaa gggcaattga gacttttcaa caaagggtaa tatccggaaa     2580

cctcctcgga ttccattgcc cagctatctg tcactttatt gtgaagatag tggaaaagga     2640

aggtggctcc tacaaatgcc atcattgcga taaaggaaag gccatcgttg aagatgcctc     2700

tgccgacagt ggtcccaaag atggaccccc acccacgagg agcatcgtgg aaaaagaaga     2760

cgttccaacc acgtcttcaa agcaagtgga ttgatgtgat atctccactg acgtaaggga     2820

tgacgcacaa tcccactatc cttcgcaaga cccttcctct atataaggaa gttcatttca     2880

tttggagagg tattaaaatc ttaataggtt ttgataaaag cgaacgtggg gaaacccgaa     2940

ccaaaccttc ttctaaactc tctctcatct ctcttaaagc aaacttctct cttgtctttc     3000

ttgcgtgagc gatcttcaac gttgtcagat cgtgcttcgg caccgcggat ggcgaaaaac     3060

gttgcgattt tcggcttatt gttttctctt cttgtgttgg ttccttctca gatcttcgcc     3120

tgcaggctcc tcagccaaaa cgacaccccc atctgtctat ccactggccc ctggatctgc     3180

tgcccaaact aactccatgg tgaccctggg atgcctggtc aagggctatt tccctgagcc     3240

agtgacagtg acctggaact ctggatccct gtccagcggt gtgcacacct tcccagctgt     3300

cctgcagtct gacctctaca ctctgagcag ctcagtgact gtcccctcca gcacctggcc     3360

cagcgagacc gtcacctgca acgttgccca cccggccagc agcaccaagg tggacaagaa     3420

aattgtgccc agggattgtg gttgtaagcc ttgcatatgt acagtcccag aagtatcatc     3480

tgtcttcatc ttccccccaa agcccaagga tgtgctcacc attactctga ctcctaaggt     3540

cacgtgtgtt gtggtagaca tcagcaagga tgatcccgag gtccagttca gctggtttgt     3600

agatgatgtg gaggtgcaca cagctcagac gcaaccccgg gaggagcagt tcaacagcac     3660

tttccgctca gtcagtgaac ttcccatcat gcaccaggac tggctcaatg gcaaggagcg     3720

atcgctcacc atcaccatca ccatcaccat caccattaaa ggcctatttt ctttagtttg     3780

aatttactgt tattcggtgt gcatttctat gtttggtgag cggttttctg tgctcagagt     3840

gtgtttattt tatgtaattt aatttctttg tgagctcctg tttagcaggt cgtcccttca     3900

gcaaggacac aaaaagattt taattttatt aaaaaaaaaa aaaaaaaaga ccgggaattc     3960

gatatcaagc ttatcgacct gcagatcgtt caaacatttg gcaataaagt ttcttaagat     4020

tgaatcctgt tgccggtctt gcgatgatta tcatataatt tctgttgaat tacgttaagc     4080

atgtaataat taacatgtaa tgcatgacgt tatttatgag atgggttttt atgattagag     4140

tcccgcaatt atacatttaa tacgcgatag aaaacaaaat atagcgcgca aactaggata     4200

aattatcgcg cgcggtgtca tctatgttac tagatctcta gagtctcaag cttggcgcgc     4260

ccacgtgact agtggcactg gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg     4320

ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt aatagcgaag     4380

aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa tgctagagca     4440

gcttgagctt ggatcagatt gtcgtttccc gccttcagtt taaactatca gtgtttgaca     4500

ggatatattg gcgggtaaac ctaagagaaa agagcgttta                           4540


<210>  6
<211>  3105
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Construct 1314 from 2X35S prom to NOS term

<400>  6
gtcaacatgg tggagcacga cacacttgtc tactccaaaa atatcaaaga tacagtctca       60

gaagaccaaa gggcaattga gacttttcaa caaagggtaa tatccggaaa cctcctcgga      120

ttccattgcc cagctatctg tcactttatt gtgaagatag tggaaaagga aggtggctcc      180

tacaaatgcc atcattgcga taaaggaaag gccatcgttg aagatgcctc tgccgacagt      240

ggtcccaaag atggaccccc acccacgagg agcatcgtgg aaaaagaaga cgttccaacc      300

acgtcttcaa agcaagtgga ttgatgtgat aacatggtgg agcacgacac acttgtctac      360

tccaaaaata tcaaagatac agtctcagaa gaccaaaggg caattgagac ttttcaacaa      420

agggtaatat ccggaaacct cctcggattc cattgcccag ctatctgtca ctttattgtg      480

aagatagtgg aaaaggaagg tggctcctac aaatgccatc attgcgataa aggaaaggcc      540

atcgttgaag atgcctctgc cgacagtggt cccaaagatg gacccccacc cacgaggagc      600

atcgtggaaa aagaagacgt tccaaccacg tcttcaaagc aagtggattg atgtgatatc      660

tccactgacg taagggatga cgcacaatcc cactatcctt cgcaagaccc ttcctctata      720

taaggaagtt catttcattt ggagaggtat taaaatctta ataggttttg ataaaagcga      780

acgtggggaa acccgaacca aaccttcttc taaactctct ctcatctctc ttaaagcaaa      840

cttctctctt gtctttcttg cgtgagcgat cttcaacgtt gtcagatcgt gcttcggcac      900

cagtacaatg gcgaaaaacg ttgcgatttt cggcttattg ttttctcttc ttgtgttggt      960

tccttctcag atcttcgctg acacattatg tataggttat catgcgaaca attcaacaga     1020

cactgtagac acagtactag aaaagaatgt aacagtaaca cactctgtta accttctaga     1080

agacaagcat aacgggaaac tatgcaaact aagaggggta gccccattgc atttgggtaa     1140

atgtaacatt gctggctgga tcctgggaaa tccagagtgt gaatcactct ccacagcaag     1200

ctcatggtcc tacattgtgg aaacacctag ttcagacaat ggaacgtgtt acccaggaga     1260

tttcatcgat tatgaggagc taagagagca attgagctca gtgtcatcat ttgaaaggtt     1320

tgagatattc cccaagacaa gttcatggcc caatcatgac tcgaacaaag gtgtaacggc     1380

agcatgtcct catgctggag caaaaagctt ctacaaaaat ttaatatggc tagttaaaaa     1440

aggaaattca tacccaaagc tcagcaaatc ctacattaat gataaaggga aagaagtcct     1500

cgtgctatgg ggcattcacc atccatctac tagtgctgac caacaaagtc tctatcagaa     1560

tgcagatgca tatgtttttg tggggtcatc aagatacagc aagaagttca agccggaaat     1620

agcaataaga cccaaagtga gggatcaaga agggagaatg aactattact ggacactagt     1680

agagccggga gacaaaataa cattcgaagc aactggaaat ctagtggtac cgagatatgc     1740

attcgcaatg gaaagaaatg ctggatctgg tattatcatt tcagatacac cagtccacga     1800

ttgcaataca acttgtcaaa cacccaaggg tgctataaac accagcctcc catttcagaa     1860

tatacatccg atcacaattg gaaaatgtcc aaaatatgta aaaagcacaa aattgagact     1920

ggccacagga ttgaggaata tcccgtctat tcaatctaga ggactatttg gggccattgc     1980

cggtttcatt gaaggggggt ggacagggat ggtagatgga tggtacggtt atcaccatca     2040

aaatgagcag gggtcaggat atgcagccga cctgaagagc acacagaatg ccattgacga     2100

gattactaac aaagtaaatt ctgttattga aaagatgaat acacagttca cagcagtagg     2160

taaagagttc aaccacctgg aaaaaagaat agagaattta aataaaaaag ttgatgatgg     2220

tttcctggac atttggactt acaatgccga actgttggtt ctattggaaa atgaaagaac     2280

tttggactac cacgattcaa atgtgaagaa cttatatgaa aaggtaagaa gccagctaaa     2340

aaacaatgcc aaggaaattg gaaacggctg ctttgaattt taccacaaat gcgataacac     2400

gtgcatggaa agtgtcaaaa atgggactta tgactaccca aaatactcag aggaagcaaa     2460

attaaacaga gaagaaatag atggggtaaa gctggaatca acaaggattt accagatttt     2520

ggcgatctat tcaactgtcg ccagttcatt ggtactggta gtctccctgg gggcaatcag     2580

tttctggatg tgctctaatg ggtctctaca gtgtagaata tgtatttaaa ggcctatttt     2640

ctttagtttg aatttactgt tattcggtgt gcatttctat gtttggtgag cggttttctg     2700

tgctcagagt gtgtttattt tatgtaattt aatttctttg tgagctcctg tttagcaggt     2760

cgtcccttca gcaaggacac aaaaagattt taattttatt aaaaaaaaaa aaaaaaaaga     2820

ccgggaattc gatatcaagc ttatcgacct gcagatcgtt caaacatttg gcaataaagt     2880

ttcttaagat tgaatcctgt tgccggtctt gcgatgatta tcatataatt tctgttgaat     2940

tacgttaagc atgtaataat taacatgtaa tgcatgacgt tatttatgag atgggttttt     3000

atgattagag tcccgcaatt atacatttaa tacgcgatag aaaacaaaat atagcgcgca     3060

aactaggata aattatcgcg cgcggtgtca tctatgttac tagat                     3105


<210>  7
<211>  49
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer H1_Cal(Y91F).r

<400>  7
aaatctcctg ggaaacacgt tccattgtct gaactaggtg tttccacaa                   49


<210>  8
<211>  49
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer H1_Cal(Y91F).c

<400>  8
agacaatgga acgtgtttcc caggagattt catcgattat gaggagcta                   49


<210>  9
<211>  4486
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Cloning vector 3637 from left to right T-DNA

<400>  9
tggcaggata tattgtggtg taaacaaatt gacgcttaga caacttaata acacattgcg       60

gacgttttta atgtactgaa ttaacgccga atcccgggct ggtatattta tatgttgtca      120

aataactcaa aaaccataaa agtttaagtt agcaagtgtg tacattttta cttgaacaaa      180

aatattcacc tactactgtt ataaatcatt attaaacatt agagtaaaga aatatggatg      240

ataagaacaa gagtagtgat attttgacaa caattttgtt gcaacatttg agaaaatttt      300

gttgttctct cttttcattg gtcaaaaaca atagagagag aaaaaggaag agggagaata      360

aaaacataat gtgagtatga gagagaaagt tgtacaaaag ttgtaccaaa atagttgtac      420

aaatatcatt gaggaatttg acaaaagcta cacaaataag ggttaattgc tgtaaataaa      480

taaggatgac gcattagaga gatgtaccat tagagaattt ttggcaagtc attaaaaaga      540

aagaataaat tatttttaaa attaaaagtt gagtcatttg attaaacatg tgattattta      600

atgaattgat gaaagagttg gattaaagtt gtattagtaa ttagaatttg gtgtcaaatt      660

taatttgaca tttgatcttt tcctatatat tgccccatag agtcagttaa ctcattttta      720

tatttcatag atcaaataag agaaataacg gtatattaat ccctccaaaa aaaaaaaacg      780

gtatatttac taaaaaatct aagccacgta ggaggataac aggatccccg taggaggata      840

acatccaatc caaccaatca caacaatcct gatgagataa cccactttaa gcccacgcat      900

ctgtggcaca tctacattat ctaaatcaca cattcttcca cacatctgag ccacacaaaa      960

accaatccac atctttatca cccattctat aaaaaatcac actttgtgag tctacacttt     1020

gattcccttc aaacacatac aaagagaaga gactaattaa ttaattaatc atcttgagag     1080

aaaatggaac gagctataca aggaaacgac gctagggaac aagctaacag tgaacgttgg     1140

gatggaggat caggaggtac cacttctccc ttcaaacttc ctgacgaaag tccgagttgg     1200

actgagtggc ggctacataa cgatgagacg aattcgaatc aagataatcc ccttggtttc     1260

aaggaaagct ggggtttcgg gaaagttgta tttaagagat atctcagata cgacaggacg     1320

gaagcttcac tgcacagagt ccttggatct tggacgggag attcggttaa ctatgcagca     1380

tctcgatttt tcggtttcga ccagatcgga tgtacctata gtattcggtt tcgaggagtt     1440

agtatcaccg tttctggagg gtcgcgaact cttcagcatc tctgtgagat ggcaattcgg     1500

tctaagcaag aactgctaca gcttgcccca atcgaagtgg aaagtaatgt atcaagagga     1560

tgccctgaag gtactcaaac cttcgaaaaa gaaagcgagt aagttaaaat gcttcttcgt     1620

ctcctattta taatatggtt tgttattgtt aattttgttc ttgtagaaga gcttaattaa     1680

tcgttgttgt tatgaaatac tatttgtatg agatgaactg gtgtaatgta attcatttac     1740

ataagtggag tcagaatcag aatgtttcct ccataactaa ctagacatga agacctgccg     1800

cgtacaattg tcttatattt gaacaactaa aattgaacat cttttgccac aactttataa     1860

gtggttaata tagctcaaat atatggtcaa gttcaataga ttaataatgg aaatatcagt     1920

tatcgaaatt cattaacaat caacttaacg ttattaacta ctaattttat atcatcccct     1980

ttgataaatg atagtacacc aattaggaag gagcatgctc gcctaggaga ttgtcgtttc     2040

ccgccttcag tttgcaagct gctctagccg tgtagccaat acgcaaaccg cctctccccg     2100

cgcgttggga attactagcg cgtgtcgaca agcttgcatg ccggtcaaca tggtggagca     2160

cgacacactt gtctactcca aaaatatcaa agatacagtc tcagaagacc aaagggcaat     2220

tgagactttt caacaaaggg taatatccgg aaacctcctc ggattccatt gcccagctat     2280

ctgtcacttt attgtgaaga tagtggaaaa ggaaggtggc tcctacaaat gccatcattg     2340

cgataaagga aaggccatcg ttgaagatgc ctctgccgac agtggtccca aagatggacc     2400

cccacccacg aggagcatcg tggaaaaaga agacgttcca accacgtctt caaagcaagt     2460

ggattgatgt gataacatgg tggagcacga cacacttgtc tactccaaaa atatcaaaga     2520

tacagtctca gaagaccaaa gggcaattga gacttttcaa caaagggtaa tatccggaaa     2580

cctcctcgga ttccattgcc cagctatctg tcactttatt gtgaagatag tggaaaagga     2640

aggtggctcc tacaaatgcc atcattgcga taaaggaaag gccatcgttg aagatgcctc     2700

tgccgacagt ggtcccaaag atggaccccc acccacgagg agcatcgtgg aaaaagaaga     2760

cgttccaacc acgtcttcaa agcaagtgga ttgatgtgat atctccactg acgtaaggga     2820

tgacgcacaa tcccactatc cttcgcaaga cccttcctct atataaggaa gttcatttca     2880

tttggagagg tattaaaatc ttaataggtt ttgataaaag cgaacgtggg gaaacccgaa     2940

ccaaaccttc ttctaaactc tctctcatct ctcttaaagc aaacttctct cttgtctttc     3000

ttgcgtgagc gatcttcaac gttgtcagat cgtgcttcgg caccgcggat ggcgaaaaac     3060

gttgcgattt tcggcttatt gttttctctt cttgtgttgg ttccttctca gatcttcgcc     3120

tgcaggctcc tcagccaaaa cgacaccccc atctgtctat ccactggccc ctggatctgc     3180

tgcccaaact aactccatgg tgaccctggg atgcctggtc aagggctatt tccctgagcc     3240

agtgacagtg acctggaact ctggatccct gtccagcggt gtgcacacct tcccagctgt     3300

cctgcagtct gacctctaca ctctgagcag ctcagtgact gtcccctcca gcacctggcc     3360

cagcgagacc gtcacctgca acgttgccca cccggccagc agcaccaagg tggacaagaa     3420

aattgtgccc agggattgtg gttgtaagcc ttgcatatgt acagtcccag aagtatcatc     3480

tgtcttcatc ttccccccaa agcccaagga tgtgctcacc attactctga ctcctaaggt     3540

cacgtgtgtt gtggtagaca tcagcaagga tgatcccgag gtccagttca gctggtttgt     3600

agatgatgtg gaggtgcaca cagctcagac gcaaccccgg gaggagcagt tcaacagcac     3660

tttccgctca gtcagtgaac ttcccatcat gcaccaggac tggctcaatg gcaaggagcg     3720

atcgctcacc atcaccatca ccatcaccat caccattaaa ggcctatttt ctttagtttg     3780

aatttactgt tattcggtgt gcatttctat gtttggtgag cggttttctg tgctcagagt     3840

gtgtttattt tatgtaattt aatttctttg tgagctcctg tttagcaggt cgtcccttca     3900

gcaaggacac aaaaagattt taattttatt atcgttcaaa catttggcaa taaagtttct     3960

taagattgaa tcctgttgcc ggtcttgcga tgattatcat ataatttctg ttgaattacg     4020

ttaagcatgt aataattaac atgtaatgca tgacgttatt tatgagatgg gtttttatga     4080

ttagagtccc gcaattatac atttaatacg cgatagaaaa caaaatatag cgcgcaaact     4140

aggataaatt atcgcgcgcg gtgtcatcta tgttactaga tctctagagt ctcaagcttg     4200

gcgcgcccac gtgactagtg gcactggccg tcgttttaca acgtcgtgac tgggaaaacc     4260

ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc tggcgtaata     4320

gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgaatgct     4380

agagcagctt gagcttggat cagattgtcg tttcccgcct tcagtttaaa ctatcagtgt     4440

ttgacaggat atattggcgg gtaaacctaa gagaaaagag cgttta                    4486


<210>  10
<211>  3051
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Construct 6100 from 2X35S prom to NOS term

<400>  10
gtcaacatgg tggagcacga cacacttgtc tactccaaaa atatcaaaga tacagtctca       60

gaagaccaaa gggcaattga gacttttcaa caaagggtaa tatccggaaa cctcctcgga      120

ttccattgcc cagctatctg tcactttatt gtgaagatag tggaaaagga aggtggctcc      180

tacaaatgcc atcattgcga taaaggaaag gccatcgttg aagatgcctc tgccgacagt      240

ggtcccaaag atggaccccc acccacgagg agcatcgtgg aaaaagaaga cgttccaacc      300

acgtcttcaa agcaagtgga ttgatgtgat aacatggtgg agcacgacac acttgtctac      360

tccaaaaata tcaaagatac agtctcagaa gaccaaaggg caattgagac ttttcaacaa      420

agggtaatat ccggaaacct cctcggattc cattgcccag ctatctgtca ctttattgtg      480

aagatagtgg aaaaggaagg tggctcctac aaatgccatc attgcgataa aggaaaggcc      540

atcgttgaag atgcctctgc cgacagtggt cccaaagatg gacccccacc cacgaggagc      600

atcgtggaaa aagaagacgt tccaaccacg tcttcaaagc aagtggattg atgtgatatc      660

tccactgacg taagggatga cgcacaatcc cactatcctt cgcaagaccc ttcctctata      720

taaggaagtt catttcattt ggagaggtat taaaatctta ataggttttg ataaaagcga      780

acgtggggaa acccgaacca aaccttcttc taaactctct ctcatctctc ttaaagcaaa      840

cttctctctt gtctttcttg cgtgagcgat cttcaacgtt gtcagatcgt gcttcggcac      900

cagtacaatg gcgaaaaacg ttgcgatttt cggcttattg ttttctcttc ttgtgttggt      960

tccttctcag atcttcgctg acacattatg tataggttat catgcgaaca attcaacaga     1020

cactgtagac acagtactag aaaagaatgt aacagtaaca cactctgtta accttctaga     1080

agacaagcat aacgggaaac tatgcaaact aagaggggta gccccattgc atttgggtaa     1140

atgtaacatt gctggctgga tcctgggaaa tccagagtgt gaatcactct ccacagcaag     1200

ctcatggtcc tacattgtgg aaacacctag ttcagacaat ggaacgtgtt tcccaggaga     1260

tttcatcgat tatgaggagc taagagagca attgagctca gtgtcatcat ttgaaaggtt     1320

tgagatattc cccaagacaa gttcatggcc caatcatgac tcgaacaaag gtgtaacggc     1380

agcatgtcct catgctggag caaaaagctt ctacaaaaat ttaatatggc tagttaaaaa     1440

aggaaattca tacccaaagc tcagcaaatc ctacattaat gataaaggga aagaagtcct     1500

cgtgctatgg ggcattcacc atccatctac tagtgctgac caacaaagtc tctatcagaa     1560

tgcagatgca tatgtttttg tggggtcatc aagatacagc aagaagttca agccggaaat     1620

agcaataaga cccaaagtga gggatcaaga agggagaatg aactattact ggacactagt     1680

agagccggga gacaaaataa cattcgaagc aactggaaat ctagtggtac cgagatatgc     1740

attcgcaatg gaaagaaatg ctggatctgg tattatcatt tcagatacac cagtccacga     1800

ttgcaataca acttgtcaaa cacccaaggg tgctataaac accagcctcc catttcagaa     1860

tatacatccg atcacaattg gaaaatgtcc aaaatatgta aaaagcacaa aattgagact     1920

ggccacagga ttgaggaata tcccgtctat tcaatctaga ggactatttg gggccattgc     1980

cggtttcatt gaaggggggt ggacagggat ggtagatgga tggtacggtt atcaccatca     2040

aaatgagcag gggtcaggat atgcagccga cctgaagagc acacagaatg ccattgacga     2100

gattactaac aaagtaaatt ctgttattga aaagatgaat acacagttca cagcagtagg     2160

taaagagttc aaccacctgg aaaaaagaat agagaattta aataaaaaag ttgatgatgg     2220

tttcctggac atttggactt acaatgccga actgttggtt ctattggaaa atgaaagaac     2280

tttggactac cacgattcaa atgtgaagaa cttatatgaa aaggtaagaa gccagctaaa     2340

aaacaatgcc aaggaaattg gaaacggctg ctttgaattt taccacaaat gcgataacac     2400

gtgcatggaa agtgtcaaaa atgggactta tgactaccca aaatactcag aggaagcaaa     2460

attaaacaga gaagaaatag atggggtaaa gctggaatca acaaggattt accagatttt     2520

ggcgatctat tcaactgtcg ccagttcatt ggtactggta gtctccctgg gggcaatcag     2580

tttctggatg tgctctaatg ggtctctaca gtgtagaata tgtatttaaa ggcctatttt     2640

ctttagtttg aatttactgt tattcggtgt gcatttctat gtttggtgag cggttttctg     2700

tgctcagagt gtgtttattt tatgtaattt aatttctttg tgagctcctg tttagcaggt     2760

cgtcccttca gcaaggacac aaaaagattt taattttatt atcgttcaaa catttggcaa     2820

taaagtttct taagattgaa tcctgttgcc ggtcttgcga tgattatcat ataatttctg     2880

ttgaattacg ttaagcatgt aataattaac atgtaatgca tgacgttatt tatgagatgg     2940

gtttttatga ttagagtccc gcaattatac atttaatacg cgatagaaaa caaaatatag     3000

cgcgcaaact aggataaatt atcgcgcgcg gtgtcatcta tgttactaga t              3051


<210>  11
<211>  1722
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PDI-H1 A/ California/7/2009 Y91F

<400>  11
atggcgaaaa acgttgcgat tttcggctta ttgttttctc ttcttgtgtt ggttccttct       60

cagatcttcg ctgacacatt atgtataggt tatcatgcga acaattcaac agacactgta      120

gacacagtac tagaaaagaa tgtaacagta acacactctg ttaaccttct agaagacaag      180

cataacggga aactatgcaa actaagaggg gtagccccat tgcatttggg taaatgtaac      240

attgctggct ggatcctggg aaatccagag tgtgaatcac tctccacagc aagctcatgg      300

tcctacattg tggaaacacc tagttcagac aatggaacgt gtttcccagg agatttcatc      360

gattatgagg agctaagaga gcaattgagc tcagtgtcat catttgaaag gtttgagata      420

ttccccaaga caagttcatg gcccaatcat gactcgaaca aaggtgtaac ggcagcatgt      480

cctcatgctg gagcaaaaag cttctacaaa aatttaatat ggctagttaa aaaaggaaat      540

tcatacccaa agctcagcaa atcctacatt aatgataaag ggaaagaagt cctcgtgcta      600

tggggcattc accatccatc tactagtgct gaccaacaaa gtctctatca gaatgcagat      660

gcatatgttt ttgtggggtc atcaagatac agcaagaagt tcaagccgga aatagcaata      720

agacccaaag tgagggatca agaagggaga atgaactatt actggacact agtagagccg      780

ggagacaaaa taacattcga agcaactgga aatctagtgg taccgagata tgcattcgca      840

atggaaagaa atgctggatc tggtattatc atttcagata caccagtcca cgattgcaat      900

acaacttgtc aaacacccaa gggtgctata aacaccagcc tcccatttca gaatatacat      960

ccgatcacaa ttggaaaatg tccaaaatat gtaaaaagca caaaattgag actggccaca     1020

ggattgagga atatcccgtc tattcaatct agaggactat ttggggccat tgccggtttc     1080

attgaagggg ggtggacagg gatggtagat ggatggtacg gttatcacca tcaaaatgag     1140

caggggtcag gatatgcagc cgacctgaag agcacacaga atgccattga cgagattact     1200

aacaaagtaa attctgttat tgaaaagatg aatacacagt tcacagcagt aggtaaagag     1260

ttcaaccacc tggaaaaaag aatagagaat ttaaataaaa aagttgatga tggtttcctg     1320

gacatttgga cttacaatgc cgaactgttg gttctattgg aaaatgaaag aactttggac     1380

taccacgatt caaatgtgaa gaacttatat gaaaaggtaa gaagccagct aaaaaacaat     1440

gccaaggaaa ttggaaacgg ctgctttgaa ttttaccaca aatgcgataa cacgtgcatg     1500

gaaagtgtca aaaatgggac ttatgactac ccaaaatact cagaggaagc aaaattaaac     1560

agagaagaaa tagatggggt aaagctggaa tcaacaagga tttaccagat tttggcgatc     1620

tattcaactg tcgccagttc attggtactg gtagtctccc tgggggcaat cagtttctgg     1680

atgtgctcta atgggtctct acagtgtaga atatgtattt aa                        1722


<210>  12
<211>  573
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  PDI-H1 A/ California/7/2009 Y91F

<400>  12

Met Ala Lys Asn Val Ala Ile Phe Gly Leu Leu Phe Ser Leu Leu Val 
1               5                   10                  15      


Leu Val Pro Ser Gln Ile Phe Ala Asp Thr Leu Cys Ile Gly Tyr His 
            20                  25                  30          


Ala Asn Asn Ser Thr Asp Thr Val Asp Thr Val Leu Glu Lys Asn Val 
        35                  40                  45              


Thr Val Thr His Ser Val Asn Leu Leu Glu Asp Lys His Asn Gly Lys 
    50                  55                  60                  


Leu Cys Lys Leu Arg Gly Val Ala Pro Leu His Leu Gly Lys Cys Asn 
65                  70                  75                  80  


Ile Ala Gly Trp Ile Leu Gly Asn Pro Glu Cys Glu Ser Leu Ser Thr 
                85                  90                  95      


Ala Ser Ser Trp Ser Tyr Ile Val Glu Thr Pro Ser Ser Asp Asn Gly 
            100                 105                 110         


Thr Cys Phe Pro Gly Asp Phe Ile Asp Tyr Glu Glu Leu Arg Glu Gln 
        115                 120                 125             


Leu Ser Ser Val Ser Ser Phe Glu Arg Phe Glu Ile Phe Pro Lys Thr 
    130                 135                 140                 


Ser Ser Trp Pro Asn His Asp Ser Asn Lys Gly Val Thr Ala Ala Cys 
145                 150                 155                 160 


Pro His Ala Gly Ala Lys Ser Phe Tyr Lys Asn Leu Ile Trp Leu Val 
                165                 170                 175     


Lys Lys Gly Asn Ser Tyr Pro Lys Leu Ser Lys Ser Tyr Ile Asn Asp 
            180                 185                 190         


Lys Gly Lys Glu Val Leu Val Leu Trp Gly Ile His His Pro Ser Thr 
        195                 200                 205             


Ser Ala Asp Gln Gln Ser Leu Tyr Gln Asn Ala Asp Ala Tyr Val Phe 
    210                 215                 220                 


Val Gly Ser Ser Arg Tyr Ser Lys Lys Phe Lys Pro Glu Ile Ala Ile 
225                 230                 235                 240 


Arg Pro Lys Val Arg Asp Gln Glu Gly Arg Met Asn Tyr Tyr Trp Thr 
                245                 250                 255     


Leu Val Glu Pro Gly Asp Lys Ile Thr Phe Glu Ala Thr Gly Asn Leu 
            260                 265                 270         


Val Val Pro Arg Tyr Ala Phe Ala Met Glu Arg Asn Ala Gly Ser Gly 
        275                 280                 285             


Ile Ile Ile Ser Asp Thr Pro Val His Asp Cys Asn Thr Thr Cys Gln 
    290                 295                 300                 


Thr Pro Lys Gly Ala Ile Asn Thr Ser Leu Pro Phe Gln Asn Ile His 
305                 310                 315                 320 


Pro Ile Thr Ile Gly Lys Cys Pro Lys Tyr Val Lys Ser Thr Lys Leu 
                325                 330                 335     


Arg Leu Ala Thr Gly Leu Arg Asn Ile Pro Ser Ile Gln Ser Arg Gly 
            340                 345                 350         


Leu Phe Gly Ala Ile Ala Gly Phe Ile Glu Gly Gly Trp Thr Gly Met 
        355                 360                 365             


Val Asp Gly Trp Tyr Gly Tyr His His Gln Asn Glu Gln Gly Ser Gly 
    370                 375                 380                 


Tyr Ala Ala Asp Leu Lys Ser Thr Gln Asn Ala Ile Asp Glu Ile Thr 
385                 390                 395                 400 


Asn Lys Val Asn Ser Val Ile Glu Lys Met Asn Thr Gln Phe Thr Ala 
                405                 410                 415     


Val Gly Lys Glu Phe Asn His Leu Glu Lys Arg Ile Glu Asn Leu Asn 
            420                 425                 430         


Lys Lys Val Asp Asp Gly Phe Leu Asp Ile Trp Thr Tyr Asn Ala Glu 
        435                 440                 445             


Leu Leu Val Leu Leu Glu Asn Glu Arg Thr Leu Asp Tyr His Asp Ser 
    450                 455                 460                 


Asn Val Lys Asn Leu Tyr Glu Lys Val Arg Ser Gln Leu Lys Asn Asn 
465                 470                 475                 480 


Ala Lys Glu Ile Gly Asn Gly Cys Phe Glu Phe Tyr His Lys Cys Asp 
                485                 490                 495     


Asn Thr Cys Met Glu Ser Val Lys Asn Gly Thr Tyr Asp Tyr Pro Lys 
            500                 505                 510         


Tyr Ser Glu Glu Ala Lys Leu Asn Arg Glu Glu Ile Asp Gly Val Lys 
        515                 520                 525             


Leu Glu Ser Thr Arg Ile Tyr Gln Ile Leu Ala Ile Tyr Ser Thr Val 
    530                 535                 540                 


Ala Ser Ser Leu Val Leu Val Val Ser Leu Gly Ala Ile Ser Phe Trp 
545                 550                 555                 560 


Met Cys Ser Asn Gly Ser Leu Gln Cys Arg Ile Cys Ile 
                565                 570             


<210>  13
<211>  550
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  A/Minnesota/41/19 (H3N2)

<400>  13

Gln Lys Ile Pro Gly Asn Asp Asn Ser Thr Ala Thr Leu Cys Leu Gly 
1               5                   10                  15      


His His Ala Val Pro Asn Gly Thr Ile Val Lys Thr Ile Thr Asn Asp 
            20                  25                  30          


Arg Ile Glu Val Thr Asn Ala Thr Glu Leu Val Gln Asn Ser Ser Ile 
        35                  40                  45              


Gly Glu Ile Cys Asp Ser Pro His Gln Ile Leu Asp Gly Gly Asn Cys 
    50                  55                  60                  


Thr Leu Ile Asp Ala Leu Leu Gly Asp Pro Gln Cys Asp Gly Phe Gln 
65                  70                  75                  80  


Asn Lys Lys Trp Asp Leu Phe Val Glu Arg Ser Arg Ala Tyr Ser Asn 
                85                  90                  95      


Cys Tyr Pro Tyr Asp Val Pro Asp Tyr Ala Ser Leu Arg Ser Leu Val 
            100                 105                 110         


Ala Ser Ser Gly Thr Leu Glu Phe Lys Asn Glu Ser Phe Asn Trp Ala 
        115                 120                 125             


Gly Val Thr Gln Asn Gly Lys Ser Phe Ser Cys Ile Arg Gly Ser Ser 
    130                 135                 140                 


Ser Ser Phe Phe Ser Arg Leu Asn Trp Leu Thr His Leu Asn Tyr Thr 
145                 150                 155                 160 


Tyr Pro Ala Leu Asn Val Thr Met Pro Asn Lys Glu Gln Phe Asp Lys 
                165                 170                 175     


Leu Tyr Ile Trp Gly Val His His Pro Gly Thr Asp Lys Asp Gln Ile 
            180                 185                 190         


Ser Leu Tyr Ala Gln Ser Ser Gly Arg Ile Thr Val Ser Thr Lys Arg 
        195                 200                 205             


Ser Gln Gln Ala Val Ile Pro Asn Ile Gly Ser Arg Pro Arg Ile Arg 
    210                 215                 220                 


Asp Ile Pro Ser Arg Ile Ser Ile Tyr Trp Thr Ile Val Lys Pro Gly 
225                 230                 235                 240 


Asp Ile Leu Leu Ile Asn Ser Thr Gly Asn Leu Ile Ala Pro Arg Gly 
                245                 250                 255     


Tyr Phe Lys Ile Arg Ser Gly Lys Ser Ser Ile Met Arg Ser Asp Ala 
            260                 265                 270         


Pro Ile Gly Lys Cys Lys Ser Glu Cys Ile Thr Pro Asn Gly Ser Ile 
        275                 280                 285             


Pro Asn Asp Lys Pro Phe Gln Asn Val Asn Arg Ile Thr Tyr Gly Ala 
    290                 295                 300                 


Cys Pro Arg Tyr Val Lys Gln Ser Thr Leu Lys Leu Ala Thr Gly Met 
305                 310                 315                 320 


Arg Asn Val Pro Glu Lys Gln Thr Arg Gly Ile Phe Gly Ala Ile Ala 
                325                 330                 335     


Gly Phe Ile Glu Asn Gly Trp Glu Gly Met Val Asp Gly Trp Tyr Gly 
            340                 345                 350         


Phe Arg His Gln Asn Ser Glu Gly Arg Gly Gln Ala Ala Asp Leu Lys 
        355                 360                 365             


Ser Thr Gln Ala Ala Ile Asp Gln Ile Asn Gly Lys Leu Asn Arg Leu 
    370                 375                 380                 


Ile Gly Lys Thr Asn Glu Lys Phe His Gln Ile Glu Lys Glu Phe Ser 
385                 390                 395                 400 


Glu Val Glu Gly Arg Val Gln Asp Leu Glu Lys Tyr Val Glu Asp Thr 
                405                 410                 415     


Lys Ile Asp Leu Trp Ser Tyr Asn Ala Glu Leu Leu Val Ala Leu Glu 
            420                 425                 430         


Asn Gln His Thr Ile Asp Leu Thr Asp Ser Glu Met Asn Lys Leu Phe 
        435                 440                 445             


Glu Lys Thr Lys Lys Gln Leu Arg Glu Asn Ala Glu Asp Met Gly Asn 
    450                 455                 460                 


Gly Cys Phe Lys Ile Tyr His Lys Cys Asp Asn Ala Cys Ile Gly Ser 
465                 470                 475                 480 


Ile Arg Asn Glu Thr Tyr Asp His Asn Val Tyr Arg Asp Glu Ala Leu 
                485                 490                 495     


Asn Asn Arg Phe Gln Ile Lys Gly Val Glu Leu Lys Ser Gly Tyr Lys 
            500                 505                 510         


Asp Trp Ile Leu Trp Ile Ser Phe Ala Ile Ser Cys Phe Leu Leu Cys 
        515                 520                 525             


Val Ala Leu Leu Gly Phe Ile Met Trp Ala Cys Gln Lys Gly Asn Ile 
    530                 535                 540                 


Arg Cys Asn Ile Cys Ile 
545                 550 


<210>  14
<211>  569
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  B/Singapore/INFKK-16-0569/16 (Yamagata)

<400>  14

Asp Arg Ile Cys Thr Gly Ile Thr Ser Ser Asn Ser Pro His Val Val 
1               5                   10                  15      


Lys Thr Ala Thr Gln Gly Glu Val Asn Val Thr Gly Val Ile Pro Leu 
            20                  25                  30          


Thr Thr Thr Pro Thr Lys Ser Tyr Phe Ala Asn Leu Lys Gly Thr Arg 
        35                  40                  45              


Thr Arg Gly Lys Leu Cys Pro Asp Cys Leu Asn Cys Thr Asp Leu Asp 
    50                  55                  60                  


Val Ala Leu Gly Arg Pro Met Cys Val Gly Thr Thr Pro Ser Ala Lys 
65                  70                  75                  80  


Ala Ser Ile Leu His Glu Val Arg Pro Val Thr Ser Gly Cys Phe Pro 
                85                  90                  95      


Ile Met His Asp Arg Thr Lys Ile Arg Gln Leu Pro Asn Leu Leu Arg 
            100                 105                 110         


Gly Tyr Glu Lys Ile Arg Leu Ser Thr Gln Asn Val Ile Asp Ala Glu 
        115                 120                 125             


Lys Ala Pro Gly Gly Pro Tyr Arg Leu Gly Thr Ser Gly Ser Cys Pro 
    130                 135                 140                 


Asn Ala Thr Ser Lys Ile Gly Phe Phe Ala Thr Met Ala Trp Ala Val 
145                 150                 155                 160 


Pro Lys Asp Asn Tyr Lys Asn Ala Thr Asn Pro Gln Thr Val Glu Val 
                165                 170                 175     


Pro Tyr Ile Cys Thr Glu Gly Glu Asp Gln Ile Thr Val Trp Gly Phe 
            180                 185                 190         


His Ser Asp Asn Lys Thr Gln Met Lys Ser Leu Tyr Gly Asp Ser Asn 
        195                 200                 205             


Pro Gln Lys Phe Thr Ser Ser Ala Asn Gly Val Thr Thr His Tyr Val 
    210                 215                 220                 


Ser Gln Ile Gly Asp Phe Pro Asp Gln Thr Glu Asp Gly Gly Leu Pro 
225                 230                 235                 240 


Gln Ser Gly Arg Ile Val Val Asp Tyr Met Val Gln Lys Pro Gly Lys 
                245                 250                 255     


Thr Gly Thr Ile Val Tyr Gln Arg Gly Val Leu Leu Pro Gln Lys Val 
            260                 265                 270         


Trp Cys Ala Ser Gly Arg Ser Lys Val Ile Lys Gly Ser Leu Pro Leu 
        275                 280                 285             


Ile Gly Glu Ala Asp Cys Leu His Glu Glu Tyr Gly Gly Leu Asn Lys 
    290                 295                 300                 


Ser Lys Pro Tyr Tyr Thr Gly Lys His Ala Lys Ala Ile Gly Asn Cys 
305                 310                 315                 320 


Pro Ile Trp Val Lys Thr Pro Leu Lys Leu Ala Asn Gly Thr Lys Tyr 
                325                 330                 335     


Arg Pro Pro Ala Lys Leu Leu Lys Glu Arg Gly Phe Phe Gly Ala Ile 
            340                 345                 350         


Ala Gly Phe Leu Glu Gly Gly Trp Glu Gly Met Ile Ala Gly Trp His 
        355                 360                 365             


Gly Tyr Thr Ser His Gly Ala His Gly Val Ala Val Ala Ala Asp Leu 
    370                 375                 380                 


Lys Ser Thr Gln Glu Ala Ile Asn Lys Ile Thr Lys Asn Leu Asn Ser 
385                 390                 395                 400 


Leu Ser Glu Leu Glu Val Lys Asn Leu Gln Arg Leu Ser Gly Ala Met 
                405                 410                 415     


Asp Glu Leu His Asn Glu Ile Leu Glu Leu Asp Glu Lys Val Asp Asp 
            420                 425                 430         


Leu Arg Ala Asp Thr Ile Ser Ser Gln Ile Glu Leu Ala Val Leu Leu 
        435                 440                 445             


Ser Asn Glu Gly Ile Ile Asn Ser Glu Asp Glu His Leu Leu Ala Leu 
    450                 455                 460                 


Glu Arg Lys Leu Lys Lys Met Leu Gly Pro Ser Ala Val Asp Ile Gly 
465                 470                 475                 480 


Asn Gly Cys Phe Glu Thr Lys His Lys Cys Asn Gln Thr Cys Leu Asp 
                485                 490                 495     


Arg Ile Ala Ala Gly Thr Phe Asn Ala Gly Glu Phe Ser Leu Pro Thr 
            500                 505                 510         


Phe Asp Ser Leu Asn Ile Thr Ala Ala Ser Leu Asn Asp Asp Gly Leu 
        515                 520                 525             


Asp Asn His Thr Ile Leu Leu Tyr Tyr Ser Thr Ala Ala Ser Ser Leu 
    530                 535                 540                 


Ala Val Thr Leu Met Leu Ala Ile Phe Ile Val Tyr Met Val Ser Arg 
545                 550                 555                 560 


Asp Asn Val Ser Cys Ser Ile Cys Leu 
                565                 


<210>  15
<211>  568
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  B/Maryland/15/16 (Victoria)

<400>  15

Asp Arg Ile Cys Thr Gly Ile Thr Ser Ser Asn Ser Pro His Val Val 
1               5                   10                  15      


Lys Thr Ala Thr Gln Gly Glu Val Asn Val Thr Gly Val Ile Pro Leu 
            20                  25                  30          


Thr Thr Thr Pro Thr Lys Ser His Phe Ala Asn Leu Lys Gly Thr Glu 
        35                  40                  45              


Thr Arg Gly Lys Leu Cys Pro Lys Cys Leu Asn Cys Thr Asp Leu Asp 
    50                  55                  60                  


Val Ala Leu Gly Arg Pro Lys Cys Thr Gly Lys Ile Pro Ser Ala Arg 
65                  70                  75                  80  


Val Ser Ile Leu His Glu Val Arg Pro Val Thr Ser Gly Cys Phe Pro 
                85                  90                  95      


Ile Met His Asp Arg Thr Lys Ile Arg Gln Leu Pro Asn Leu Leu Arg 
            100                 105                 110         


Gly Tyr Glu His Val Arg Leu Ser Thr His Asn Val Ile Asn Ala Glu 
        115                 120                 125             


Asp Ala Pro Gly Gly Pro Tyr Lys Ile Gly Thr Ser Gly Ser Cys Pro 
    130                 135                 140                 


Asn Ile Thr Asn Gly Asn Gly Phe Phe Ala Thr Met Ala Trp Ala Val 
145                 150                 155                 160 


Pro Asp Lys Asn Lys Thr Ala Thr Asn Pro Leu Thr Ile Glu Val Pro 
                165                 170                 175     


Tyr Val Cys Thr Glu Gly Glu Asp Gln Ile Thr Val Trp Gly Phe His 
            180                 185                 190         


Ser Asp Asn Glu Thr Gln Met Ala Lys Leu Tyr Gly Asp Ser Lys Pro 
        195                 200                 205             


Gln Lys Phe Thr Ser Ser Ala Asn Gly Val Thr Thr His Tyr Val Ser 
    210                 215                 220                 


Gln Ile Gly Gly Phe Pro Asn Gln Thr Glu Asp Gly Gly Leu Pro Gln 
225                 230                 235                 240 


Ser Gly Arg Ile Val Val Asp Tyr Met Val Gln Lys Ser Gly Lys Thr 
                245                 250                 255     


Gly Thr Ile Thr Tyr Gln Arg Gly Ile Leu Leu Pro Gln Lys Val Trp 
            260                 265                 270         


Cys Ala Ser Gly Arg Ser Lys Val Ile Lys Gly Ser Leu Pro Leu Ile 
        275                 280                 285             


Gly Glu Ala Asp Cys Leu His Glu Lys Tyr Gly Gly Leu Asn Lys Ser 
    290                 295                 300                 


Lys Pro Tyr Tyr Thr Gly Glu His Ala Lys Ala Ile Gly Asn Cys Pro 
305                 310                 315                 320 


Ile Trp Val Lys Thr Pro Leu Lys Leu Ala Asn Gly Thr Lys Tyr Arg 
                325                 330                 335     


Pro Pro Ala Lys Leu Leu Lys Glu Arg Gly Phe Phe Gly Ala Ile Ala 
            340                 345                 350         


Gly Phe Leu Glu Gly Gly Trp Glu Gly Met Ile Ala Gly Trp His Gly 
        355                 360                 365             


Tyr Thr Ser His Gly Ala His Gly Val Ala Val Ala Ala Asp Leu Lys 
    370                 375                 380                 


Ser Thr Gln Glu Ala Ile Asn Lys Ile Thr Lys Asn Leu Asn Ser Leu 
385                 390                 395                 400 


Ser Glu Leu Glu Val Lys Asn Leu Gln Arg Leu Ser Gly Ala Met Asp 
                405                 410                 415     


Glu Leu His Asn Glu Ile Leu Glu Leu Asp Glu Lys Val Asp Asp Leu 
            420                 425                 430         


Arg Ala Asp Thr Ile Ser Ser Gln Ile Glu Leu Ala Val Leu Leu Ser 
        435                 440                 445             


Asn Glu Gly Ile Ile Asn Ser Glu Asp Glu His Leu Leu Ala Leu Glu 
    450                 455                 460                 


Arg Lys Leu Lys Lys Met Leu Gly Pro Ser Ala Val Glu Ile Gly Asn 
465                 470                 475                 480 


Gly Cys Phe Glu Thr Lys His Lys Cys Asn Gln Thr Cys Leu Asp Lys 
                485                 490                 495     


Ile Ala Ala Gly Thr Phe Asp Ala Gly Glu Phe Ser Leu Pro Thr Phe 
            500                 505                 510         


Asp Ser Leu Asn Ile Thr Ala Ala Ser Leu Asn Asp Asp Gly Leu Asp 
        515                 520                 525             


Asn His Thr Ile Leu Leu Tyr Tyr Ser Thr Ala Ala Ser Ser Leu Ala 
    530                 535                 540                 


Val Thr Leu Met Ile Ala Ile Phe Val Val Tyr Met Val Ser Arg Asp 
545                 550                 555                 560 


Asn Val Ser Cys Ser Ile Cys Leu 
                565             


<210>  16
<211>  567
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  B/Victoria/705/18 (Victoria)

<400>  16

Asp Arg Ile Cys Thr Gly Ile Thr Ser Ser Asn Ser Pro His Val Val 
1               5                   10                  15      


Lys Thr Ala Thr Gln Gly Glu Val Asn Val Thr Gly Val Ile Pro Leu 
            20                  25                  30          


Thr Thr Thr Pro Thr Lys Ser His Phe Ala Asn Leu Lys Gly Thr Glu 
        35                  40                  45              


Thr Arg Gly Lys Leu Cys Pro Lys Cys Leu Asn Cys Thr Asp Leu Asp 
    50                  55                  60                  


Val Ala Leu Ser Arg Pro Lys Cys Thr Gly Lys Ile Pro Ser Ala Arg 
65                  70                  75                  80  


Val Ser Ile Leu His Glu Val Arg Pro Val Thr Ser Gly Cys Phe Pro 
                85                  90                  95      


Ile Met His Asp Arg Thr Lys Ile Arg Gln Leu Pro Asn Leu Leu Arg 
            100                 105                 110         


Gly Tyr Glu His Val Arg Leu Ser Thr His Asn Val Ile Asn Ala Glu 
        115                 120                 125             


Asp Ala Pro Gly Gly Pro Tyr Glu Ile Gly Thr Ser Gly Ser Cys Pro 
    130                 135                 140                 


Asn Ile Thr Asn Gly Asn Gly Phe Phe Ala Thr Met Ala Trp Ala Val 
145                 150                 155                 160 


Pro Lys Asn Lys Thr Ala Thr Asn Pro Leu Thr Ile Glu Val Pro Tyr 
                165                 170                 175     


Ile Cys Thr Glu Gly Glu Asp Gln Ile Thr Val Trp Gly Phe His Ser 
            180                 185                 190         


Asp Asn Glu Thr Gln Met Ala Lys Leu Tyr Gly Asp Ser Lys Pro Gln 
        195                 200                 205             


Lys Phe Thr Ser Ser Ala Asn Gly Val Thr Thr His Tyr Val Ser Gln 
    210                 215                 220                 


Ile Gly Gly Phe Pro Asn Gln Thr Glu Asp Gly Gly Leu Pro Gln Ser 
225                 230                 235                 240 


Gly Arg Ile Val Val Asp Tyr Met Val Gln Lys Ser Gly Lys Thr Gly 
                245                 250                 255     


Thr Ile Thr Tyr Gln Arg Gly Ile Leu Leu Pro Gln Lys Val Trp Cys 
            260                 265                 270         


Ala Ser Gly Arg Ser Lys Val Ile Lys Gly Ser Leu Pro Leu Ile Gly 
        275                 280                 285             


Glu Ala Asp Cys Leu His Glu Lys Tyr Gly Gly Leu Asn Lys Ser Lys 
    290                 295                 300                 


Pro Tyr Tyr Thr Gly Glu His Ala Lys Ala Ile Gly Asn Cys Pro Ile 
305                 310                 315                 320 


Trp Val Lys Thr Pro Leu Lys Leu Ala Asn Gly Thr Lys Tyr Arg Pro 
                325                 330                 335     


Pro Ala Lys Leu Leu Lys Glu Arg Gly Phe Phe Gly Ala Ile Ala Gly 
            340                 345                 350         


Phe Leu Glu Gly Gly Trp Glu Gly Met Ile Ala Gly Trp His Gly Tyr 
        355                 360                 365             


Thr Ser His Gly Ala His Gly Val Ala Val Ala Ala Asp Leu Lys Ser 
    370                 375                 380                 


Thr Gln Glu Ala Ile Asn Lys Ile Thr Lys Asn Leu Asn Ser Leu Ser 
385                 390                 395                 400 


Glu Leu Glu Val Lys Asn Leu Gln Arg Leu Ser Gly Ala Met Asp Glu 
                405                 410                 415     


Leu His Asn Glu Ile Leu Glu Leu Asp Glu Lys Val Asp Asp Leu Arg 
            420                 425                 430         


Ala Asp Thr Ile Ser Ser Gln Ile Glu Leu Ala Val Leu Leu Ser Asn 
        435                 440                 445             


Glu Gly Ile Ile Asn Ser Glu Asp Glu His Leu Leu Ala Leu Glu Arg 
    450                 455                 460                 


Lys Leu Lys Lys Met Leu Gly Pro Ser Ala Val Glu Ile Gly Asn Gly 
465                 470                 475                 480 


Cys Phe Glu Thr Lys His Lys Cys Asn Gln Thr Cys Leu Asp Arg Ile 
                485                 490                 495     


Ala Ala Gly Thr Phe Asp Ala Gly Glu Phe Ser Leu Pro Thr Phe Asp 
            500                 505                 510         


Ser Leu Asn Ile Thr Ala Ala Ser Leu Asn Asp Asp Gly Leu Asp Asn 
        515                 520                 525             


His Thr Ile Leu Leu Tyr Tyr Ser Thr Ala Ala Ser Ser Leu Ala Val 
    530                 535                 540                 


Thr Leu Met Ile Ala Ile Phe Val Val Tyr Met Val Ser Arg Asp Ser 
545                 550                 555                 560 


Val Ser Cys Ser Ile Cys Leu 
                565         


<210>  17
<211>  567
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  B/Washington/12/19 (Victoria)

<400>  17

Asp Arg Ile Cys Thr Gly Ile Thr Ser Ser Asn Ser Pro His Val Val 
1               5                   10                  15      


Lys Thr Ala Thr Gln Gly Glu Val Asn Val Thr Gly Val Ile Pro Leu 
            20                  25                  30          


Thr Thr Thr Pro Thr Lys Ser His Phe Ala Asn Leu Lys Gly Thr Glu 
        35                  40                  45              


Thr Arg Gly Lys Leu Cys Pro Lys Cys Leu Asn Cys Thr Asp Leu Asp 
    50                  55                  60                  


Val Ala Leu Gly Arg Pro Lys Cys Thr Gly Lys Ile Pro Ser Ala Arg 
65                  70                  75                  80  


Val Ser Ile Leu His Glu Val Arg Pro Val Thr Ser Gly Cys Phe Pro 
                85                  90                  95      


Ile Met His Asp Arg Thr Lys Ile Arg Gln Leu Pro Asn Leu Leu Arg 
            100                 105                 110         


Gly Tyr Glu His Val Arg Leu Ser Thr His Asn Val Ile Asn Ala Glu 
        115                 120                 125             


Asp Ala Pro Gly Arg Pro Tyr Glu Ile Gly Thr Ser Gly Ser Cys Pro 
    130                 135                 140                 


Asn Ile Thr Asn Gly Asn Gly Phe Phe Ala Thr Met Ala Trp Ala Val 
145                 150                 155                 160 


Pro Lys Asn Lys Thr Ala Thr Asn Pro Leu Thr Ile Glu Val Pro Tyr 
                165                 170                 175     


Ile Cys Thr Glu Gly Glu Asp Gln Ile Thr Val Trp Gly Phe His Ser 
            180                 185                 190         


Asp Asn Glu Thr Gln Met Ala Lys Leu Tyr Gly Asp Ser Lys Pro Gln 
        195                 200                 205             


Lys Phe Thr Ser Ser Ala Asn Gly Val Thr Thr His Tyr Val Ser Gln 
    210                 215                 220                 


Ile Gly Gly Phe Pro Asn Gln Thr Glu Asp Gly Gly Leu Pro Gln Ser 
225                 230                 235                 240 


Gly Arg Ile Val Val Asp Tyr Met Val Gln Lys Ser Gly Lys Thr Gly 
                245                 250                 255     


Thr Ile Thr Tyr Gln Arg Gly Ile Leu Leu Pro Gln Lys Val Trp Cys 
            260                 265                 270         


Ala Ser Gly Arg Ser Lys Val Ile Lys Gly Ser Leu Pro Leu Ile Gly 
        275                 280                 285             


Glu Ala Asp Cys Leu His Glu Lys Tyr Gly Gly Leu Asn Lys Ser Lys 
    290                 295                 300                 


Pro Tyr Tyr Thr Gly Glu His Ala Lys Ala Ile Gly Asn Cys Pro Ile 
305                 310                 315                 320 


Trp Val Lys Thr Pro Leu Lys Leu Ala Asn Gly Thr Lys Tyr Arg Pro 
                325                 330                 335     


Pro Ala Lys Leu Leu Lys Glu Arg Gly Phe Phe Gly Ala Ile Ala Gly 
            340                 345                 350         


Phe Leu Glu Gly Gly Trp Glu Gly Met Ile Ala Gly Trp His Gly Tyr 
        355                 360                 365             


Thr Ser His Gly Ala His Gly Val Ala Val Ala Ala Asp Leu Lys Ser 
    370                 375                 380                 


Thr Gln Glu Ala Ile Asn Lys Ile Thr Lys Asn Leu Asn Ser Leu Ser 
385                 390                 395                 400 


Glu Leu Glu Val Lys Asn Leu Gln Arg Leu Ser Gly Ala Met Asp Glu 
                405                 410                 415     


Leu His Asn Glu Ile Leu Glu Leu Asp Glu Lys Val Asp Asp Leu Arg 
            420                 425                 430         


Ala Asp Thr Ile Ser Ser Gln Ile Glu Leu Ala Val Leu Leu Ser Asn 
        435                 440                 445             


Glu Gly Ile Ile Asn Ser Glu Asp Glu His Leu Leu Ala Leu Glu Arg 
    450                 455                 460                 


Lys Leu Lys Lys Met Leu Gly Pro Ser Ala Val Glu Ile Gly Asn Gly 
465                 470                 475                 480 


Cys Phe Glu Thr Lys His Lys Cys Asn Gln Thr Cys Leu Asp Arg Ile 
                485                 490                 495     


Ala Ala Gly Thr Phe Asp Ala Gly Glu Phe Ser Leu Pro Thr Phe Asp 
            500                 505                 510         


Ser Leu Asn Ile Thr Ala Ala Ser Leu Asn Asp Asp Gly Leu Asp Asn 
        515                 520                 525             


His Thr Ile Leu Leu Tyr Tyr Ser Thr Ala Ala Ser Ser Leu Ala Val 
    530                 535                 540                 


Thr Leu Met Ile Ala Ile Phe Val Val Tyr Met Val Ser Arg Asp Asn 
545                 550                 555                 560 


Val Ser Cys Ser Ile Cys Leu 
                565         


<210>  18
<211>  567
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  B/Darwin/8/19 (Victoria)

<400>  18

Asp Arg Ile Cys Thr Gly Ile Thr Ser Ser Asn Ser Pro His Val Val 
1               5                   10                  15      


Lys Thr Ala Thr Gln Gly Glu Val Asn Val Thr Gly Val Ile Pro Leu 
            20                  25                  30          


Thr Thr Thr Pro Thr Lys Ser His Phe Ala Asn Leu Lys Gly Thr Glu 
        35                  40                  45              


Thr Arg Gly Lys Leu Cys Pro Lys Cys Leu Asn Cys Thr Asp Leu Asp 
    50                  55                  60                  


Val Ala Leu Gly Arg Pro Lys Cys Thr Gly Lys Ile Pro Ser Ala Arg 
65                  70                  75                  80  


Val Ser Ile Leu His Glu Val Arg Pro Val Thr Ser Gly Cys Phe Pro 
                85                  90                  95      


Ile Met His Asp Arg Thr Lys Ile Arg Gln Leu Pro Asn Leu Leu Arg 
            100                 105                 110         


Gly Tyr Glu His Val Arg Leu Ser Thr His Asn Val Ile Asn Ala Glu 
        115                 120                 125             


Asp Ala Pro Gly Arg Pro Tyr Glu Ile Gly Thr Ser Gly Ser Cys Pro 
    130                 135                 140                 


Asn Ile Thr Asn Gly Asn Gly Phe Phe Ala Thr Met Ala Trp Ala Val 
145                 150                 155                 160 


Pro Lys Asn Lys Thr Ala Thr Asn Pro Leu Thr Ile Glu Val Pro Tyr 
                165                 170                 175     


Ile Cys Thr Glu Gly Glu Asp Gln Ile Thr Val Trp Gly Phe His Ser 
            180                 185                 190         


Asp Asn Glu Ala Gln Met Ala Lys Leu Tyr Gly Asp Ser Lys Pro Gln 
        195                 200                 205             


Lys Phe Thr Ser Ser Ala Asn Gly Val Thr Thr His Tyr Val Ser Gln 
    210                 215                 220                 


Ile Gly Gly Phe Pro Asn Gln Thr Glu Asp Gly Gly Leu Pro Gln Ser 
225                 230                 235                 240 


Gly Arg Ile Val Val Asp Tyr Met Val Gln Lys Ser Gly Lys Thr Gly 
                245                 250                 255     


Thr Ile Thr Tyr Gln Arg Gly Ile Leu Leu Pro Gln Lys Val Trp Cys 
            260                 265                 270         


Ala Ser Gly Arg Ser Lys Val Ile Lys Gly Ser Leu Pro Leu Ile Gly 
        275                 280                 285             


Glu Ala Asp Cys Leu His Glu Lys Tyr Gly Gly Leu Asn Lys Ser Lys 
    290                 295                 300                 


Pro Tyr Tyr Thr Gly Glu His Ala Lys Ala Ile Gly Asn Cys Pro Ile 
305                 310                 315                 320 


Trp Val Lys Thr Pro Leu Lys Leu Ala Asn Gly Thr Lys Tyr Arg Pro 
                325                 330                 335     


Pro Ala Lys Leu Leu Lys Glu Arg Gly Phe Phe Gly Ala Ile Ala Gly 
            340                 345                 350         


Phe Leu Glu Gly Gly Trp Glu Gly Met Ile Ala Gly Trp His Gly Tyr 
        355                 360                 365             


Thr Ser His Gly Ala His Gly Val Ala Val Ala Ala Asp Leu Lys Ser 
    370                 375                 380                 


Thr Gln Glu Ala Ile Asn Lys Ile Thr Lys Asn Leu Asn Ser Leu Ser 
385                 390                 395                 400 


Glu Leu Glu Val Lys Asn Leu Gln Arg Leu Ser Gly Ala Met Asp Glu 
                405                 410                 415     


Leu His Asn Glu Ile Leu Glu Leu Asp Glu Lys Val Asp Asp Leu Arg 
            420                 425                 430         


Ala Asp Thr Ile Ser Ser Gln Ile Glu Leu Ala Val Leu Leu Ser Asn 
        435                 440                 445             


Glu Gly Ile Ile Asn Ser Glu Asp Glu His Leu Leu Ala Leu Glu Arg 
    450                 455                 460                 


Lys Leu Lys Lys Met Leu Gly Pro Ser Ala Val Glu Ile Gly Asn Gly 
465                 470                 475                 480 


Cys Phe Glu Thr Lys His Lys Cys Asn Gln Thr Cys Leu Asp Arg Ile 
                485                 490                 495     


Ala Ala Gly Thr Phe Asp Ala Gly Glu Phe Ser Leu Pro Thr Phe Asp 
            500                 505                 510         


Ser Leu Asn Ile Thr Ala Ala Ser Leu Asn Asp Asp Gly Leu Asp Asn 
        515                 520                 525             


His Thr Ile Leu Leu Tyr Tyr Ser Thr Ala Ala Ser Ser Leu Ala Val 
    530                 535                 540                 


Thr Leu Met Ile Ala Ile Phe Val Val Tyr Met Val Ser Arg Asp Asn 
545                 550                 555                 560 


Val Ser Cys Ser Ile Cys Leu 
                565         


<210>  19
<211>  567
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  B/Darwin/20/19 (Victoria)

<400>  19

Asp Arg Ile Cys Thr Gly Ile Thr Ser Ser Asn Ser Pro His Val Val 
1               5                   10                  15      


Lys Thr Ala Thr Gln Gly Glu Val Asn Val Thr Gly Val Ile Pro Leu 
            20                  25                  30          


Thr Thr Thr Pro Thr Lys Ser His Phe Ala Asn Leu Lys Gly Thr Lys 
        35                  40                  45              


Thr Arg Gly Lys Leu Cys Pro Lys Cys Leu Asn Cys Thr Asp Leu Asp 
    50                  55                  60                  


Val Ala Leu Gly Arg Pro Lys Cys Thr Gly Lys Ile Pro Ser Ala Arg 
65                  70                  75                  80  


Val Ser Ile Leu His Glu Val Arg Pro Val Thr Ser Gly Cys Phe Pro 
                85                  90                  95      


Ile Met His Asp Arg Thr Lys Ile Arg Gln Leu Pro Asn Leu Leu Arg 
            100                 105                 110         


Gly Tyr Glu His Val Arg Leu Ser Thr His Asn Val Ile Asn Ala Glu 
        115                 120                 125             


Asp Ala Pro Gly Arg Pro Tyr Glu Ile Gly Thr Ser Gly Ser Cys Pro 
    130                 135                 140                 


Asn Ile Thr Asn Gly Asn Gly Phe Phe Ala Thr Met Ala Trp Ala Val 
145                 150                 155                 160 


Pro Lys Asn Lys Thr Ala Thr Asn Pro Leu Thr Ile Glu Val Pro Tyr 
                165                 170                 175     


Ile Cys Thr Glu Gly Glu Asp Gln Ile Thr Val Trp Gly Phe His Ser 
            180                 185                 190         


Asp Asn Glu Ile Gln Met Ala Lys Leu Tyr Gly Asp Ser Lys Pro Gln 
        195                 200                 205             


Lys Phe Thr Ser Ser Ala Asn Gly Val Thr Thr His Tyr Val Ser Gln 
    210                 215                 220                 


Ile Gly Gly Phe Pro Asn Gln Thr Glu Asp Gly Gly Leu Pro Gln Ser 
225                 230                 235                 240 


Gly Arg Ile Val Val Asp Tyr Met Val Gln Lys Ser Gly Lys Thr Gly 
                245                 250                 255     


Thr Ile Thr Tyr Gln Arg Gly Ile Leu Leu Pro Gln Lys Val Trp Cys 
            260                 265                 270         


Ala Ser Gly Arg Ser Lys Val Ile Lys Gly Ser Leu Pro Leu Ile Gly 
        275                 280                 285             


Glu Ala Asp Cys Leu His Glu Lys Tyr Gly Gly Leu Asn Lys Ser Lys 
    290                 295                 300                 


Pro Tyr Tyr Thr Gly Glu His Ala Lys Ala Ile Gly Asn Cys Pro Ile 
305                 310                 315                 320 


Trp Val Lys Thr Pro Leu Lys Leu Ala Asn Gly Thr Lys Tyr Arg Pro 
                325                 330                 335     


Pro Ala Lys Leu Leu Lys Glu Arg Gly Phe Phe Gly Ala Ile Ala Gly 
            340                 345                 350         


Phe Leu Glu Gly Gly Trp Glu Gly Met Ile Ala Gly Trp His Gly Tyr 
        355                 360                 365             


Thr Ser His Gly Ala His Gly Val Ala Val Ala Ala Asp Leu Lys Ser 
    370                 375                 380                 


Thr Gln Glu Ala Ile Asn Lys Ile Thr Lys Asn Leu Asn Ser Leu Ser 
385                 390                 395                 400 


Glu Leu Glu Val Lys Asn Leu Gln Arg Leu Ser Gly Ala Met Asp Glu 
                405                 410                 415     


Leu His Asn Glu Ile Leu Glu Leu Asp Glu Lys Val Asp Asp Leu Arg 
            420                 425                 430         


Ala Asp Thr Ile Ser Ser Gln Ile Glu Leu Ala Val Leu Leu Ser Asn 
        435                 440                 445             


Glu Gly Ile Ile Asn Ser Glu Asp Glu His Leu Leu Ala Leu Glu Arg 
    450                 455                 460                 


Lys Leu Lys Lys Met Leu Gly Pro Ser Ala Val Glu Ile Gly Asn Gly 
465                 470                 475                 480 


Cys Phe Glu Thr Lys His Lys Cys Asn Gln Thr Cys Leu Asp Arg Ile 
                485                 490                 495     


Ala Ala Gly Thr Phe Asp Ala Gly Glu Phe Ser Leu Pro Thr Phe Asp 
            500                 505                 510         


Ser Leu Asn Ile Thr Ala Ala Ser Leu Asn Asp Asp Gly Leu Asp Asn 
        515                 520                 525             


His Thr Ile Leu Leu Tyr Tyr Ser Thr Ala Ala Ser Ser Leu Ala Val 
    530                 535                 540                 


Thr Leu Met Ile Ala Ile Phe Val Val Tyr Met Val Ser Arg Asp Asn 
545                 550                 555                 560 


Val Ser Cys Ser Ile Cys Leu 
                565         


<210>  20
<211>  1701
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PDI H7 A/Shanghai/2/2013

<400>  20
atggcgaaaa acgttgcgat tttcggctta ttgttttctc ttcttgtgtt ggttccttct       60

cagatcttcg cggacaaaat ctgcctcgga catcatgccg tgtcaaacgg aaccaaagta      120

aacacattaa ctgaaagagg agtggaagtc gtcaatgcaa ctgaaacagt ggaacgaaca      180

aacatcccca ggatctgctc aaaagggaaa aggacagttg acctcggtca atgtggactc      240

ctggggacaa tcactggacc acctcaatgt gaccaattcc tagaattttc agccgattta      300

attattgaga ggcgagaagg aagtgatgtc tgttatcctg ggaaattcgt gaatgaagaa      360

gctctgaggc aaattctcag agaatcaggc ggaattgaca aggaagcaat gggattcaca      420

tacagtggaa taagaactaa tggagcaacc agtgcatgta ggagatcagg atcttcattc      480

tatgcagaaa tgaaatggct cctgtcaaac acagataatg ctgcattccc gcagatgact      540

aagtcatata aaaatacaag aaaaagccca gctctaatag tatgggggat ccatcattcc      600

gtatcaactg cagagcaaac caagctatat gggagtggaa acaaactggt gacagttggg      660

agttctaatt atcaacaatc ttttgtaccg agtccaggag cgagaccaca agttaatggt      720

ctgtctggaa gaattgactt tcattggcta atgctaaatc ccaatgatac agtcactttc      780

agtttcaatg gggctttcat agctccagac cgtgcaagct tcctgagagg aaaatctatg      840

ggaatccaga gtggagtaca ggttgatgcc aattgtgaag gggactgcta tcatagtgga      900

gggacaataa taagtaactt gccatttcag aacatagata gcagggcagt tggaaaatgt      960

ccgagatatg ttaagcaaag gagtctgctg ctagcaacag ggatgaagaa tgttcctgag     1020

attccaaagg gaagaggcct atttggtgct atagcgggtt tcattgaaaa tggatgggaa     1080

ggcctaattg atggttggta tggtttcaga caccagaatg cacagggaga gggaactgct     1140

gcagattaca aaagcactca atcggcaatt gatcaaataa caggaaaatt aaaccggctt     1200

atagaaaaaa ccaaccaaca atttgagttg atcgacaatg aattcaatga ggtagagaag     1260

caaatcggta atgtgataaa ttggaccaga gattctataa cagaagtgtg gtcatacaat     1320

gctgaactct tggtagcaat ggagaaccag catacaattg atctggctga ttcagaaatg     1380

gacaaactgt acgaacgagt gaaaagacag ctgagagaga atgctgaaga agatggcact     1440

ggttgctttg aaatatttca caagtgtgat gatgactgta tggccagtat tagaaataac     1500

acctatgatc acagcaaata cagggaagag gcaatgcaaa atagaataca gattgaccca     1560

gtcaaactaa gcagcggcta caaagatgtg atactttggt ttagcttcgg ggcatcatgt     1620

ttcatacttc tagccattgt aatgggcctt gtcttcatat gtgtaaagaa tggaaacatg     1680

cggtgcacta tttgtatata a                                               1701


<210>  21
<211>  566
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  PDI H7 A/Shanghai/2/2013

<400>  21

Met Ala Lys Asn Val Ala Ile Phe Gly Leu Leu Phe Ser Leu Leu Val 
1               5                   10                  15      


Leu Val Pro Ser Gln Ile Phe Ala Asp Lys Ile Cys Leu Gly His His 
            20                  25                  30          


Ala Val Ser Asn Gly Thr Lys Val Asn Thr Leu Thr Glu Arg Gly Val 
        35                  40                  45              


Glu Val Val Asn Ala Thr Glu Thr Val Glu Arg Thr Asn Ile Pro Arg 
    50                  55                  60                  


Ile Cys Ser Lys Gly Lys Arg Thr Val Asp Leu Gly Gln Cys Gly Leu 
65                  70                  75                  80  


Leu Gly Thr Ile Thr Gly Pro Pro Gln Cys Asp Gln Phe Leu Glu Phe 
                85                  90                  95      


Ser Ala Asp Leu Ile Ile Glu Arg Arg Glu Gly Ser Asp Val Cys Tyr 
            100                 105                 110         


Pro Gly Lys Phe Val Asn Glu Glu Ala Leu Arg Gln Ile Leu Arg Glu 
        115                 120                 125             


Ser Gly Gly Ile Asp Lys Glu Ala Met Gly Phe Thr Tyr Ser Gly Ile 
    130                 135                 140                 


Arg Thr Asn Gly Ala Thr Ser Ala Cys Arg Arg Ser Gly Ser Ser Phe 
145                 150                 155                 160 


Tyr Ala Glu Met Lys Trp Leu Leu Ser Asn Thr Asp Asn Ala Ala Phe 
                165                 170                 175     


Pro Gln Met Thr Lys Ser Tyr Lys Asn Thr Arg Lys Ser Pro Ala Leu 
            180                 185                 190         


Ile Val Trp Gly Ile His His Ser Val Ser Thr Ala Glu Gln Thr Lys 
        195                 200                 205             


Leu Tyr Gly Ser Gly Asn Lys Leu Val Thr Val Gly Ser Ser Asn Tyr 
    210                 215                 220                 


Gln Gln Ser Phe Val Pro Ser Pro Gly Ala Arg Pro Gln Val Asn Gly 
225                 230                 235                 240 


Leu Ser Gly Arg Ile Asp Phe His Trp Leu Met Leu Asn Pro Asn Asp 
                245                 250                 255     


Thr Val Thr Phe Ser Phe Asn Gly Ala Phe Ile Ala Pro Asp Arg Ala 
            260                 265                 270         


Ser Phe Leu Arg Gly Lys Ser Met Gly Ile Gln Ser Gly Val Gln Val 
        275                 280                 285             


Asp Ala Asn Cys Glu Gly Asp Cys Tyr His Ser Gly Gly Thr Ile Ile 
    290                 295                 300                 


Ser Asn Leu Pro Phe Gln Asn Ile Asp Ser Arg Ala Val Gly Lys Cys 
305                 310                 315                 320 


Pro Arg Tyr Val Lys Gln Arg Ser Leu Leu Leu Ala Thr Gly Met Lys 
                325                 330                 335     


Asn Val Pro Glu Ile Pro Lys Gly Arg Gly Leu Phe Gly Ala Ile Ala 
            340                 345                 350         


Gly Phe Ile Glu Asn Gly Trp Glu Gly Leu Ile Asp Gly Trp Tyr Gly 
        355                 360                 365             


Phe Arg His Gln Asn Ala Gln Gly Glu Gly Thr Ala Ala Asp Tyr Lys 
    370                 375                 380                 


Ser Thr Gln Ser Ala Ile Asp Gln Ile Thr Gly Lys Leu Asn Arg Leu 
385                 390                 395                 400 


Ile Glu Lys Thr Asn Gln Gln Phe Glu Leu Ile Asp Asn Glu Phe Asn 
                405                 410                 415     


Glu Val Glu Lys Gln Ile Gly Asn Val Ile Asn Trp Thr Arg Asp Ser 
            420                 425                 430         


Ile Thr Glu Val Trp Ser Tyr Asn Ala Glu Leu Leu Val Ala Met Glu 
        435                 440                 445             


Asn Gln His Thr Ile Asp Leu Ala Asp Ser Glu Met Asp Lys Leu Tyr 
    450                 455                 460                 


Glu Arg Val Lys Arg Gln Leu Arg Glu Asn Ala Glu Glu Asp Gly Thr 
465                 470                 475                 480 


Gly Cys Phe Glu Ile Phe His Lys Cys Asp Asp Asp Cys Met Ala Ser 
                485                 490                 495     


Ile Arg Asn Asn Thr Tyr Asp His Ser Lys Tyr Arg Glu Glu Ala Met 
            500                 505                 510         


Gln Asn Arg Ile Gln Ile Asp Pro Val Lys Leu Ser Ser Gly Tyr Lys 
        515                 520                 525             


Asp Val Ile Leu Trp Phe Ser Phe Gly Ala Ser Cys Phe Ile Leu Leu 
    530                 535                 540                 


Ala Ile Val Met Gly Leu Val Phe Ile Cys Val Lys Asn Gly Asn Met 
545                 550                 555                 560 


Arg Cys Thr Ile Cys Ile 
                565     


<210>  22
<211>  50
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer IF-H7Shang.r

<400>  22
actaaagaaa ataggccttt atatacaaat agtgcaccgc atgtttccat                  50


<210>  23
<211>  50
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer H7Shang(Y88F).c

<400>  23
aggaagtgat gtctgtttcc ctgggaaatt cgtgaatgaa gaagctctga                  50


<210>  24
<211>  50
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer H7Shang(Y88F).r

<400>  24
acgaatttcc cagggaaaca gacatcactt ccttctcgcc tctcaataat                  50


<210>  25
<211>  1701
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PDI H7 A/Shanghai/2/2013 Y88F

<400>  25
atggcgaaaa acgttgcgat tttcggctta ttgttttctc ttcttgtgtt ggttccttct       60

cagatcttcg cggacaaaat ctgcctcgga catcatgccg tgtcaaacgg aaccaaagta      120

aacacattaa ctgaaagagg agtggaagtc gtcaatgcaa ctgaaacagt ggaacgaaca      180

aacatcccca ggatctgctc aaaagggaaa aggacagttg acctcggtca atgtggactc      240

ctggggacaa tcactggacc acctcaatgt gaccaattcc tagaattttc agccgattta      300

attattgaga ggcgagaagg aagtgatgtc tgtttccctg ggaaattcgt gaatgaagaa      360

gctctgaggc aaattctcag agaatcaggc ggaattgaca aggaagcaat gggattcaca      420

tacagtggaa taagaactaa tggagcaacc agtgcatgta ggagatcagg atcttcattc      480

tatgcagaaa tgaaatggct cctgtcaaac acagataatg ctgcattccc gcagatgact      540

aagtcatata aaaatacaag aaaaagccca gctctaatag tatgggggat ccatcattcc      600

gtatcaactg cagagcaaac caagctatat gggagtggaa acaaactggt gacagttggg      660

agttctaatt atcaacaatc ttttgtaccg agtccaggag cgagaccaca agttaatggt      720

ctgtctggaa gaattgactt tcattggcta atgctaaatc ccaatgatac agtcactttc      780

agtttcaatg gggctttcat agctccagac cgtgcaagct tcctgagagg aaaatctatg      840

ggaatccaga gtggagtaca ggttgatgcc aattgtgaag gggactgcta tcatagtgga      900

gggacaataa taagtaactt gccatttcag aacatagata gcagggcagt tggaaaatgt      960

ccgagatatg ttaagcaaag gagtctgctg ctagcaacag ggatgaagaa tgttcctgag     1020

attccaaagg gaagaggcct atttggtgct atagcgggtt tcattgaaaa tggatgggaa     1080

ggcctaattg atggttggta tggtttcaga caccagaatg cacagggaga gggaactgct     1140

gcagattaca aaagcactca atcggcaatt gatcaaataa caggaaaatt aaaccggctt     1200

atagaaaaaa ccaaccaaca atttgagttg atcgacaatg aattcaatga ggtagagaag     1260

caaatcggta atgtgataaa ttggaccaga gattctataa cagaagtgtg gtcatacaat     1320

gctgaactct tggtagcaat ggagaaccag catacaattg atctggctga ttcagaaatg     1380

gacaaactgt acgaacgagt gaaaagacag ctgagagaga atgctgaaga agatggcact     1440

ggttgctttg aaatatttca caagtgtgat gatgactgta tggccagtat tagaaataac     1500

acctatgatc acagcaaata cagggaagag gcaatgcaaa atagaataca gattgaccca     1560

gtcaaactaa gcagcggcta caaagatgtg atactttggt ttagcttcgg ggcatcatgt     1620

ttcatacttc tagccattgt aatgggcctt gtcttcatat gtgtaaagaa tggaaacatg     1680

cggtgcacta tttgtatata a                                               1701


<210>  26
<211>  566
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  PDI H7 A/Shanghai/2/2013 Y88F

<400>  26

Met Ala Lys Asn Val Ala Ile Phe Gly Leu Leu Phe Ser Leu Leu Val 
1               5                   10                  15      


Leu Val Pro Ser Gln Ile Phe Ala Asp Lys Ile Cys Leu Gly His His 
            20                  25                  30          


Ala Val Ser Asn Gly Thr Lys Val Asn Thr Leu Thr Glu Arg Gly Val 
        35                  40                  45              


Glu Val Val Asn Ala Thr Glu Thr Val Glu Arg Thr Asn Ile Pro Arg 
    50                  55                  60                  


Ile Cys Ser Lys Gly Lys Arg Thr Val Asp Leu Gly Gln Cys Gly Leu 
65                  70                  75                  80  


Leu Gly Thr Ile Thr Gly Pro Pro Gln Cys Asp Gln Phe Leu Glu Phe 
                85                  90                  95      


Ser Ala Asp Leu Ile Ile Glu Arg Arg Glu Gly Ser Asp Val Cys Phe 
            100                 105                 110         


Pro Gly Lys Phe Val Asn Glu Glu Ala Leu Arg Gln Ile Leu Arg Glu 
        115                 120                 125             


Ser Gly Gly Ile Asp Lys Glu Ala Met Gly Phe Thr Tyr Ser Gly Ile 
    130                 135                 140                 


Arg Thr Asn Gly Ala Thr Ser Ala Cys Arg Arg Ser Gly Ser Ser Phe 
145                 150                 155                 160 


Tyr Ala Glu Met Lys Trp Leu Leu Ser Asn Thr Asp Asn Ala Ala Phe 
                165                 170                 175     


Pro Gln Met Thr Lys Ser Tyr Lys Asn Thr Arg Lys Ser Pro Ala Leu 
            180                 185                 190         


Ile Val Trp Gly Ile His His Ser Val Ser Thr Ala Glu Gln Thr Lys 
        195                 200                 205             


Leu Tyr Gly Ser Gly Asn Lys Leu Val Thr Val Gly Ser Ser Asn Tyr 
    210                 215                 220                 


Gln Gln Ser Phe Val Pro Ser Pro Gly Ala Arg Pro Gln Val Asn Gly 
225                 230                 235                 240 


Leu Ser Gly Arg Ile Asp Phe His Trp Leu Met Leu Asn Pro Asn Asp 
                245                 250                 255     


Thr Val Thr Phe Ser Phe Asn Gly Ala Phe Ile Ala Pro Asp Arg Ala 
            260                 265                 270         


Ser Phe Leu Arg Gly Lys Ser Met Gly Ile Gln Ser Gly Val Gln Val 
        275                 280                 285             


Asp Ala Asn Cys Glu Gly Asp Cys Tyr His Ser Gly Gly Thr Ile Ile 
    290                 295                 300                 


Ser Asn Leu Pro Phe Gln Asn Ile Asp Ser Arg Ala Val Gly Lys Cys 
305                 310                 315                 320 


Pro Arg Tyr Val Lys Gln Arg Ser Leu Leu Leu Ala Thr Gly Met Lys 
                325                 330                 335     


Asn Val Pro Glu Ile Pro Lys Gly Arg Gly Leu Phe Gly Ala Ile Ala 
            340                 345                 350         


Gly Phe Ile Glu Asn Gly Trp Glu Gly Leu Ile Asp Gly Trp Tyr Gly 
        355                 360                 365             


Phe Arg His Gln Asn Ala Gln Gly Glu Gly Thr Ala Ala Asp Tyr Lys 
    370                 375                 380                 


Ser Thr Gln Ser Ala Ile Asp Gln Ile Thr Gly Lys Leu Asn Arg Leu 
385                 390                 395                 400 


Ile Glu Lys Thr Asn Gln Gln Phe Glu Leu Ile Asp Asn Glu Phe Asn 
                405                 410                 415     


Glu Val Glu Lys Gln Ile Gly Asn Val Ile Asn Trp Thr Arg Asp Ser 
            420                 425                 430         


Ile Thr Glu Val Trp Ser Tyr Asn Ala Glu Leu Leu Val Ala Met Glu 
        435                 440                 445             


Asn Gln His Thr Ile Asp Leu Ala Asp Ser Glu Met Asp Lys Leu Tyr 
    450                 455                 460                 


Glu Arg Val Lys Arg Gln Leu Arg Glu Asn Ala Glu Glu Asp Gly Thr 
465                 470                 475                 480 


Gly Cys Phe Glu Ile Phe His Lys Cys Asp Asp Asp Cys Met Ala Ser 
                485                 490                 495     


Ile Arg Asn Asn Thr Tyr Asp His Ser Lys Tyr Arg Glu Glu Ala Met 
            500                 505                 510         


Gln Asn Arg Ile Gln Ile Asp Pro Val Lys Leu Ser Ser Gly Tyr Lys 
        515                 520                 525             


Asp Val Ile Leu Trp Phe Ser Phe Gly Ala Ser Cys Phe Ile Leu Leu 
    530                 535                 540                 


Ala Ile Val Met Gly Leu Val Phe Ile Cys Val Lys Asn Gly Asn Met 
545                 550                 555                 560 


Arg Cys Thr Ile Cys Ile 
                565     


<210>  27
<211>  1731
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PDI B/Phuket/3073/2013

<400>  27
atggcgaaaa acgttgcgat tttcggctta ttgttttctc ttcttgtgtt ggttccttct       60

cagatcttcg cggatcgaat ctgcactggg ataacatctt caaactcacc tcatgtggtc      120

aaaacagcta ctcaagggga ggtcaatgtg actggcgtga taccactgac aacaacacca      180

acaaaatctt attttgcaaa tctcaaagga acaaggacca gagggaaact atgcccggac      240

tgtctcaact gtacagatct ggatgtggcc ttgggcaggc caatgtgtgt ggggaccaca      300

ccttctgcta aagcttcaat actccatgag gtcagacctg ttacatccgg gtgctttcct      360

ataatgcacg acagaacaaa aatcaggcaa ctacccaatc ttctcagagg atatgaaaag      420

atcaggttat caacccaaaa cgttatcgat gcagaaaaag caccaggagg accctacaga      480

cttggaacct caggatcttg ccctaacgct accagtaaaa tcggattttt tgcaacaatg      540

gcttgggctg tcccaaagga caactacaaa aatgcaacga acccactaac agtggaagta      600

ccatacattt gtacagaagg ggaagaccaa attactgttt gggggttcca ttcggataac      660

aaaacccaaa tgaagagcct ctatggagac tcaaatcctc aaaagttcac ctcatctgct      720

aatggagtaa ccacgcatta tgtttctcag attggcgact tcccagatca aacagaagac      780

ggaggactac cacaaagcgg cagaattgtt gttgattaca tgatgcaaaa acctgggaaa      840

acaggaacaa ttgtctatca aaggggtgtt ttgttgcctc aaaaggtgtg gtgcgcgagt      900

ggcaggagca aagtaataaa agggtcattg cctttaattg gtgaagcaga ttgccttcat      960

gaagaatacg gtggattaaa caaaagcaag ccttactaca caggaaaaca tgcaaaagcc     1020

ataggaaatt gcccaatatg ggtaaaaaca cctttgaagc ttgccaatgg aaccaaatat     1080

agacctcctg gtggaggatg ggaaggaatg attgcaggtt ggcacggata cacatctcac     1140

ggagcacatg gagtggcagt ggcggcagac cttaagagta cacaagaagc tataaataag     1200

ataacaaaaa atctcaattc tttgagtgaa ctagaagtaa agaaccttca aagactaagt     1260

ggtgccatgg atgaactcca caacgaaata ctcgagctgg atgaaaaagt ggatgatctc     1320

agagctgaca ctataagctc acaaatagaa cttgcagtct tgctttccaa cgaaggaata     1380

ataaacagtg aagacgagca tctattggca cttgagagaa aactaaagaa aatgctgggt     1440

ccctctgctg tagacatagg aaacggatgc ttcgaaacca aacacaaatg caaccagacc     1500

tgcttagaca ggatagctgc tggcaccttt aatgcaggag aattttctct ccccactttt     1560

gattcattga acattactgc tgcatcttta aatgatgatg gattggataa ctaccagatt     1620

ttggcgatct attcaactgt cgccagttca ttggtactgg tagtctccct gggggcaatc     1680

agtttctgga tgtgctctaa tgggtctcta cagtgtagaa tatgtattta a              1731


<210>  28
<211>  576
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  PDI B/Phuket/3073/2013)

<400>  28

Met Ala Lys Asn Val Ala Ile Phe Gly Leu Leu Phe Ser Leu Leu Val 
1               5                   10                  15      


Leu Val Pro Ser Gln Ile Phe Ala Asp Arg Ile Cys Thr Gly Ile Thr 
            20                  25                  30          


Ser Ser Asn Ser Pro His Val Val Lys Thr Ala Thr Gln Gly Glu Val 
        35                  40                  45              


Asn Val Thr Gly Val Ile Pro Leu Thr Thr Thr Pro Thr Lys Ser Tyr 
    50                  55                  60                  


Phe Ala Asn Leu Lys Gly Thr Arg Thr Arg Gly Lys Leu Cys Pro Asp 
65                  70                  75                  80  


Cys Leu Asn Cys Thr Asp Leu Asp Val Ala Leu Gly Arg Pro Met Cys 
                85                  90                  95      


Val Gly Thr Thr Pro Ser Ala Lys Ala Ser Ile Leu His Glu Val Arg 
            100                 105                 110         


Pro Val Thr Ser Gly Cys Phe Pro Ile Met His Asp Arg Thr Lys Ile 
        115                 120                 125             


Arg Gln Leu Pro Asn Leu Leu Arg Gly Tyr Glu Lys Ile Arg Leu Ser 
    130                 135                 140                 


Thr Gln Asn Val Ile Asp Ala Glu Lys Ala Pro Gly Gly Pro Tyr Arg 
145                 150                 155                 160 


Leu Gly Thr Ser Gly Ser Cys Pro Asn Ala Thr Ser Lys Ile Gly Phe 
                165                 170                 175     


Phe Ala Thr Met Ala Trp Ala Val Pro Lys Asp Asn Tyr Lys Asn Ala 
            180                 185                 190         


Thr Asn Pro Leu Thr Val Glu Val Pro Tyr Ile Cys Thr Glu Gly Glu 
        195                 200                 205             


Asp Gln Ile Thr Val Trp Gly Phe His Ser Asp Asn Lys Thr Gln Met 
    210                 215                 220                 


Lys Ser Leu Tyr Gly Asp Ser Asn Pro Gln Lys Phe Thr Ser Ser Ala 
225                 230                 235                 240 


Asn Gly Val Thr Thr His Tyr Val Ser Gln Ile Gly Asp Phe Pro Asp 
                245                 250                 255     


Gln Thr Glu Asp Gly Gly Leu Pro Gln Ser Gly Arg Ile Val Val Asp 
            260                 265                 270         


Tyr Met Met Gln Lys Pro Gly Lys Thr Gly Thr Ile Val Tyr Gln Arg 
        275                 280                 285             


Gly Val Leu Leu Pro Gln Lys Val Trp Cys Ala Ser Gly Arg Ser Lys 
    290                 295                 300                 


Val Ile Lys Gly Ser Leu Pro Leu Ile Gly Glu Ala Asp Cys Leu His 
305                 310                 315                 320 


Glu Glu Tyr Gly Gly Leu Asn Lys Ser Lys Pro Tyr Tyr Thr Gly Lys 
                325                 330                 335     


His Ala Lys Ala Ile Gly Asn Cys Pro Ile Trp Val Lys Thr Pro Leu 
            340                 345                 350         


Lys Leu Ala Asn Gly Thr Lys Tyr Arg Pro Pro Gly Gly Gly Trp Glu 
        355                 360                 365             


Gly Met Ile Ala Gly Trp His Gly Tyr Thr Ser His Gly Ala His Gly 
    370                 375                 380                 


Val Ala Val Ala Ala Asp Leu Lys Ser Thr Gln Glu Ala Ile Asn Lys 
385                 390                 395                 400 


Ile Thr Lys Asn Leu Asn Ser Leu Ser Glu Leu Glu Val Lys Asn Leu 
                405                 410                 415     


Gln Arg Leu Ser Gly Ala Met Asp Glu Leu His Asn Glu Ile Leu Glu 
            420                 425                 430         


Leu Asp Glu Lys Val Asp Asp Leu Arg Ala Asp Thr Ile Ser Ser Gln 
        435                 440                 445             


Ile Glu Leu Ala Val Leu Leu Ser Asn Glu Gly Ile Ile Asn Ser Glu 
    450                 455                 460                 


Asp Glu His Leu Leu Ala Leu Glu Arg Lys Leu Lys Lys Met Leu Gly 
465                 470                 475                 480 


Pro Ser Ala Val Asp Ile Gly Asn Gly Cys Phe Glu Thr Lys His Lys 
                485                 490                 495     


Cys Asn Gln Thr Cys Leu Asp Arg Ile Ala Ala Gly Thr Phe Asn Ala 
            500                 505                 510         


Gly Glu Phe Ser Leu Pro Thr Phe Asp Ser Leu Asn Ile Thr Ala Ala 
        515                 520                 525             


Ser Leu Asn Asp Asp Gly Leu Asp Asn Tyr Gln Ile Leu Ala Ile Tyr 
    530                 535                 540                 


Ser Thr Val Ala Ser Ser Leu Val Leu Val Val Ser Leu Gly Ala Ile 
545                 550                 555                 560 


Ser Phe Trp Met Cys Ser Asn Gly Ser Leu Gln Cys Arg Ile Cys Ile 
                565                 570                 575     


<210>  29
<211>  52
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer IF.HBPhu3073.c

<400>  29
tctcagatct tcgcggatcg aatctgcact gggataacat cttcaaactc ac               52


<210>  30
<211>  59
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer B_Phuket(S140A).c

<400>  30
gaccctacag acttggaacc gccggatctt gccctaacgc taccagtaaa atcggattt        59


<210>  31
<211>  57
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer B_Phuket(S140A).r

<400>  31
cgttagggca agatccggcg gttccaagtc tgtagggtcc tcctggtgct ttttctg          57


<210>  32
<211>  1731
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PDI B/Phuket/3073/2013 S140A

<400>  32
atggcgaaaa acgttgcgat tttcggctta ttgttttctc ttcttgtgtt ggttccttct       60

cagatcttcg cggatcgaat ctgcactggg ataacatctt caaactcacc tcatgtggtc      120

aaaacagcta ctcaagggga ggtcaatgtg actggcgtga taccactgac aacaacacca      180

acaaaatctt attttgcaaa tctcaaagga acaaggacca gagggaaact atgcccggac      240

tgtctcaact gtacagatct ggatgtggcc ttgggcaggc caatgtgtgt ggggaccaca      300

ccttctgcta aagcttcaat actccatgag gtcagacctg ttacatccgg gtgctttcct      360

ataatgcacg acagaacaaa aatcaggcaa ctacccaatc ttctcagagg atatgaaaag      420

atcaggttat caacccaaaa cgttatcgat gcagaaaaag caccaggagg accctacaga      480

cttggaaccg ccggatcttg ccctaacgct accagtaaaa tcggattttt tgcaacaatg      540

gcttgggctg tcccaaagga caactacaaa aatgcaacga acccactaac agtggaagta      600

ccatacattt gtacagaagg ggaagaccaa attactgttt gggggttcca ttcggataac      660

aaaacccaaa tgaagagcct ctatggagac tcaaatcctc aaaagttcac ctcatctgct      720

aatggagtaa ccacgcatta tgtttctcag attggcgact tcccagatca aacagaagac      780

ggaggactac cacaaagcgg cagaattgtt gttgattaca tgatgcaaaa acctgggaaa      840

acaggaacaa ttgtctatca aaggggtgtt ttgttgcctc aaaaggtgtg gtgcgcgagt      900

ggcaggagca aagtaataaa agggtcattg cctttaattg gtgaagcaga ttgccttcat      960

gaagaatacg gtggattaaa caaaagcaag ccttactaca caggaaaaca tgcaaaagcc     1020

ataggaaatt gcccaatatg ggtaaaaaca cctttgaagc ttgccaatgg aaccaaatat     1080

agacctcctg gtggaggatg ggaaggaatg attgcaggtt ggcacggata cacatctcac     1140

ggagcacatg gagtggcagt ggcggcagac cttaagagta cacaagaagc tataaataag     1200

ataacaaaaa atctcaattc tttgagtgaa ctagaagtaa agaaccttca aagactaagt     1260

ggtgccatgg atgaactcca caacgaaata ctcgagctgg atgaaaaagt ggatgatctc     1320

agagctgaca ctataagctc acaaatagaa cttgcagtct tgctttccaa cgaaggaata     1380

ataaacagtg aagacgagca tctattggca cttgagagaa aactaaagaa aatgctgggt     1440

ccctctgctg tagacatagg aaacggatgc ttcgaaacca aacacaaatg caaccagacc     1500

tgcttagaca ggatagctgc tggcaccttt aatgcaggag aattttctct ccccactttt     1560

gattcattga acattactgc tgcatcttta aatgatgatg gattggataa ctaccagatt     1620

ttggcgatct attcaactgt cgccagttca ttggtactgg tagtctccct gggggcaatc     1680

agtttctgga tgtgctctaa tgggtctcta cagtgtagaa tatgtattta a              1731


<210>  33
<211>  576
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  PDI B/Phuket/3073/2013 S140A

<400>  33

Met Ala Lys Asn Val Ala Ile Phe Gly Leu Leu Phe Ser Leu Leu Val 
1               5                   10                  15      


Leu Val Pro Ser Gln Ile Phe Ala Asp Arg Ile Cys Thr Gly Ile Thr 
            20                  25                  30          


Ser Ser Asn Ser Pro His Val Val Lys Thr Ala Thr Gln Gly Glu Val 
        35                  40                  45              


Asn Val Thr Gly Val Ile Pro Leu Thr Thr Thr Pro Thr Lys Ser Tyr 
    50                  55                  60                  


Phe Ala Asn Leu Lys Gly Thr Arg Thr Arg Gly Lys Leu Cys Pro Asp 
65                  70                  75                  80  


Cys Leu Asn Cys Thr Asp Leu Asp Val Ala Leu Gly Arg Pro Met Cys 
                85                  90                  95      


Val Gly Thr Thr Pro Ser Ala Lys Ala Ser Ile Leu His Glu Val Arg 
            100                 105                 110         


Pro Val Thr Ser Gly Cys Phe Pro Ile Met His Asp Arg Thr Lys Ile 
        115                 120                 125             


Arg Gln Leu Pro Asn Leu Leu Arg Gly Tyr Glu Lys Ile Arg Leu Ser 
    130                 135                 140                 


Thr Gln Asn Val Ile Asp Ala Glu Lys Ala Pro Gly Gly Pro Tyr Arg 
145                 150                 155                 160 


Leu Gly Thr Ala Gly Ser Cys Pro Asn Ala Thr Ser Lys Ile Gly Phe 
                165                 170                 175     


Phe Ala Thr Met Ala Trp Ala Val Pro Lys Asp Asn Tyr Lys Asn Ala 
            180                 185                 190         


Thr Asn Pro Leu Thr Val Glu Val Pro Tyr Ile Cys Thr Glu Gly Glu 
        195                 200                 205             


Asp Gln Ile Thr Val Trp Gly Phe His Ser Asp Asn Lys Thr Gln Met 
    210                 215                 220                 


Lys Ser Leu Tyr Gly Asp Ser Asn Pro Gln Lys Phe Thr Ser Ser Ala 
225                 230                 235                 240 


Asn Gly Val Thr Thr His Tyr Val Ser Gln Ile Gly Asp Phe Pro Asp 
                245                 250                 255     


Gln Thr Glu Asp Gly Gly Leu Pro Gln Ser Gly Arg Ile Val Val Asp 
            260                 265                 270         


Tyr Met Met Gln Lys Pro Gly Lys Thr Gly Thr Ile Val Tyr Gln Arg 
        275                 280                 285             


Gly Val Leu Leu Pro Gln Lys Val Trp Cys Ala Ser Gly Arg Ser Lys 
    290                 295                 300                 


Val Ile Lys Gly Ser Leu Pro Leu Ile Gly Glu Ala Asp Cys Leu His 
305                 310                 315                 320 


Glu Glu Tyr Gly Gly Leu Asn Lys Ser Lys Pro Tyr Tyr Thr Gly Lys 
                325                 330                 335     


His Ala Lys Ala Ile Gly Asn Cys Pro Ile Trp Val Lys Thr Pro Leu 
            340                 345                 350         


Lys Leu Ala Asn Gly Thr Lys Tyr Arg Pro Pro Gly Gly Gly Trp Glu 
        355                 360                 365             


Gly Met Ile Ala Gly Trp His Gly Tyr Thr Ser His Gly Ala His Gly 
    370                 375                 380                 


Val Ala Val Ala Ala Asp Leu Lys Ser Thr Gln Glu Ala Ile Asn Lys 
385                 390                 395                 400 


Ile Thr Lys Asn Leu Asn Ser Leu Ser Glu Leu Glu Val Lys Asn Leu 
                405                 410                 415     


Gln Arg Leu Ser Gly Ala Met Asp Glu Leu His Asn Glu Ile Leu Glu 
            420                 425                 430         


Leu Asp Glu Lys Val Asp Asp Leu Arg Ala Asp Thr Ile Ser Ser Gln 
        435                 440                 445             


Ile Glu Leu Ala Val Leu Leu Ser Asn Glu Gly Ile Ile Asn Ser Glu 
    450                 455                 460                 


Asp Glu His Leu Leu Ala Leu Glu Arg Lys Leu Lys Lys Met Leu Gly 
465                 470                 475                 480 


Pro Ser Ala Val Asp Ile Gly Asn Gly Cys Phe Glu Thr Lys His Lys 
                485                 490                 495     


Cys Asn Gln Thr Cys Leu Asp Arg Ile Ala Ala Gly Thr Phe Asn Ala 
            500                 505                 510         


Gly Glu Phe Ser Leu Pro Thr Phe Asp Ser Leu Asn Ile Thr Ala Ala 
        515                 520                 525             


Ser Leu Asn Asp Asp Gly Leu Asp Asn Tyr Gln Ile Leu Ala Ile Tyr 
    530                 535                 540                 


Ser Thr Val Ala Ser Ser Leu Val Leu Val Val Ser Leu Gly Ala Ile 
545                 550                 555                 560 


Ser Phe Trp Met Cys Ser Asn Gly Ser Leu Gln Cys Arg Ile Cys Ile 
                565                 570                 575     


<210>  34
<211>  58
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer B_Phuket(S142A).c

<400>  34
tggaacctca ggagcctgcc ctaacgctac cagtaaaatc ggattttttg caacaatg         58


<210>  35
<211>  48
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer B_Phuket(S142A).r

<400>  35
tggtagcgtt agggcaggct cctgaggttc caagtctgta gggtcctc                    48


<210>  36
<211>  1731
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PDI B/Phuket/3073/2013 S142A

<400>  36
atggcgaaaa acgttgcgat tttcggctta ttgttttctc ttcttgtgtt ggttccttct       60

cagatcttcg cggatcgaat ctgcactggg ataacatctt caaactcacc tcatgtggtc      120

aaaacagcta ctcaagggga ggtcaatgtg actggcgtga taccactgac aacaacacca      180

acaaaatctt attttgcaaa tctcaaagga acaaggacca gagggaaact atgcccggac      240

tgtctcaact gtacagatct ggatgtggcc ttgggcaggc caatgtgtgt ggggaccaca      300

ccttctgcta aagcttcaat actccatgag gtcagacctg ttacatccgg gtgctttcct      360

ataatgcacg acagaacaaa aatcaggcaa ctacccaatc ttctcagagg atatgaaaag      420

atcaggttat caacccaaaa cgttatcgat gcagaaaaag caccaggagg accctacaga      480

cttggaacct caggagcctg ccctaacgct accagtaaaa tcggattttt tgcaacaatg      540

gcttgggctg tcccaaagga caactacaaa aatgcaacga acccactaac agtggaagta      600

ccatacattt gtacagaagg ggaagaccaa attactgttt gggggttcca ttcggataac      660

aaaacccaaa tgaagagcct ctatggagac tcaaatcctc aaaagttcac ctcatctgct      720

aatggagtaa ccacgcatta tgtttctcag attggcgact tcccagatca aacagaagac      780

ggaggactac cacaaagcgg cagaattgtt gttgattaca tgatgcaaaa acctgggaaa      840

acaggaacaa ttgtctatca aaggggtgtt ttgttgcctc aaaaggtgtg gtgcgcgagt      900

ggcaggagca aagtaataaa agggtcattg cctttaattg gtgaagcaga ttgccttcat      960

gaagaatacg gtggattaaa caaaagcaag ccttactaca caggaaaaca tgcaaaagcc     1020

ataggaaatt gcccaatatg ggtaaaaaca cctttgaagc ttgccaatgg aaccaaatat     1080

agacctcctg gtggaggatg ggaaggaatg attgcaggtt ggcacggata cacatctcac     1140

ggagcacatg gagtggcagt ggcggcagac cttaagagta cacaagaagc tataaataag     1200

ataacaaaaa atctcaattc tttgagtgaa ctagaagtaa agaaccttca aagactaagt     1260

ggtgccatgg atgaactcca caacgaaata ctcgagctgg atgaaaaagt ggatgatctc     1320

agagctgaca ctataagctc acaaatagaa cttgcagtct tgctttccaa cgaaggaata     1380

ataaacagtg aagacgagca tctattggca cttgagagaa aactaaagaa aatgctgggt     1440

ccctctgctg tagacatagg aaacggatgc ttcgaaacca aacacaaatg caaccagacc     1500

tgcttagaca ggatagctgc tggcaccttt aatgcaggag aattttctct ccccactttt     1560

gattcattga acattactgc tgcatcttta aatgatgatg gattggataa ctaccagatt     1620

ttggcgatct attcaactgt cgccagttca ttggtactgg tagtctccct gggggcaatc     1680

agtttctgga tgtgctctaa tgggtctcta cagtgtagaa tatgtattta a              1731


<210>  37
<211>  576
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  PDI B/Phuket/3073/2013 S142A

<400>  37

Met Ala Lys Asn Val Ala Ile Phe Gly Leu Leu Phe Ser Leu Leu Val 
1               5                   10                  15      


Leu Val Pro Ser Gln Ile Phe Ala Asp Arg Ile Cys Thr Gly Ile Thr 
            20                  25                  30          


Ser Ser Asn Ser Pro His Val Val Lys Thr Ala Thr Gln Gly Glu Val 
        35                  40                  45              


Asn Val Thr Gly Val Ile Pro Leu Thr Thr Thr Pro Thr Lys Ser Tyr 
    50                  55                  60                  


Phe Ala Asn Leu Lys Gly Thr Arg Thr Arg Gly Lys Leu Cys Pro Asp 
65                  70                  75                  80  


Cys Leu Asn Cys Thr Asp Leu Asp Val Ala Leu Gly Arg Pro Met Cys 
                85                  90                  95      


Val Gly Thr Thr Pro Ser Ala Lys Ala Ser Ile Leu His Glu Val Arg 
            100                 105                 110         


Pro Val Thr Ser Gly Cys Phe Pro Ile Met His Asp Arg Thr Lys Ile 
        115                 120                 125             


Arg Gln Leu Pro Asn Leu Leu Arg Gly Tyr Glu Lys Ile Arg Leu Ser 
    130                 135                 140                 


Thr Gln Asn Val Ile Asp Ala Glu Lys Ala Pro Gly Gly Pro Tyr Arg 
145                 150                 155                 160 


Leu Gly Thr Ser Gly Ala Cys Pro Asn Ala Thr Ser Lys Ile Gly Phe 
                165                 170                 175     


Phe Ala Thr Met Ala Trp Ala Val Pro Lys Asp Asn Tyr Lys Asn Ala 
            180                 185                 190         


Thr Asn Pro Leu Thr Val Glu Val Pro Tyr Ile Cys Thr Glu Gly Glu 
        195                 200                 205             


Asp Gln Ile Thr Val Trp Gly Phe His Ser Asp Asn Lys Thr Gln Met 
    210                 215                 220                 


Lys Ser Leu Tyr Gly Asp Ser Asn Pro Gln Lys Phe Thr Ser Ser Ala 
225                 230                 235                 240 


Asn Gly Val Thr Thr His Tyr Val Ser Gln Ile Gly Asp Phe Pro Asp 
                245                 250                 255     


Gln Thr Glu Asp Gly Gly Leu Pro Gln Ser Gly Arg Ile Val Val Asp 
            260                 265                 270         


Tyr Met Met Gln Lys Pro Gly Lys Thr Gly Thr Ile Val Tyr Gln Arg 
        275                 280                 285             


Gly Val Leu Leu Pro Gln Lys Val Trp Cys Ala Ser Gly Arg Ser Lys 
    290                 295                 300                 


Val Ile Lys Gly Ser Leu Pro Leu Ile Gly Glu Ala Asp Cys Leu His 
305                 310                 315                 320 


Glu Glu Tyr Gly Gly Leu Asn Lys Ser Lys Pro Tyr Tyr Thr Gly Lys 
                325                 330                 335     


His Ala Lys Ala Ile Gly Asn Cys Pro Ile Trp Val Lys Thr Pro Leu 
            340                 345                 350         


Lys Leu Ala Asn Gly Thr Lys Tyr Arg Pro Pro Gly Gly Gly Trp Glu 
        355                 360                 365             


Gly Met Ile Ala Gly Trp His Gly Tyr Thr Ser His Gly Ala His Gly 
    370                 375                 380                 


Val Ala Val Ala Ala Asp Leu Lys Ser Thr Gln Glu Ala Ile Asn Lys 
385                 390                 395                 400 


Ile Thr Lys Asn Leu Asn Ser Leu Ser Glu Leu Glu Val Lys Asn Leu 
                405                 410                 415     


Gln Arg Leu Ser Gly Ala Met Asp Glu Leu His Asn Glu Ile Leu Glu 
            420                 425                 430         


Leu Asp Glu Lys Val Asp Asp Leu Arg Ala Asp Thr Ile Ser Ser Gln 
        435                 440                 445             


Ile Glu Leu Ala Val Leu Leu Ser Asn Glu Gly Ile Ile Asn Ser Glu 
    450                 455                 460                 


Asp Glu His Leu Leu Ala Leu Glu Arg Lys Leu Lys Lys Met Leu Gly 
465                 470                 475                 480 


Pro Ser Ala Val Asp Ile Gly Asn Gly Cys Phe Glu Thr Lys His Lys 
                485                 490                 495     


Cys Asn Gln Thr Cys Leu Asp Arg Ile Ala Ala Gly Thr Phe Asn Ala 
            500                 505                 510         


Gly Glu Phe Ser Leu Pro Thr Phe Asp Ser Leu Asn Ile Thr Ala Ala 
        515                 520                 525             


Ser Leu Asn Asp Asp Gly Leu Asp Asn Tyr Gln Ile Leu Ala Ile Tyr 
    530                 535                 540                 


Ser Thr Val Ala Ser Ser Leu Val Leu Val Val Ser Leu Gly Ala Ile 
545                 550                 555                 560 


Ser Phe Trp Met Cys Ser Asn Gly Ser Leu Gln Cys Arg Ile Cys Ile 
                565                 570                 575     


<210>  38
<211>  49
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer B_Phuket(G138A).c

<400>  38
gaccctacag acttgccacc tcaggatctt gccctaacgc taccagtaa                   49


<210>  39
<211>  51
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer B_Phuket(G138A).r

<400>  39
ggcaagatcc tgaggtggca agtctgtagg gtcctcctgg tgctttttct g                51


<210>  40
<211>  1731
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PDI B/Phuket/3073/2013 G138A

<400>  40
atggcgaaaa acgttgcgat tttcggctta ttgttttctc ttcttgtgtt ggttccttct       60

cagatcttcg cggatcgaat ctgcactggg ataacatctt caaactcacc tcatgtggtc      120

aaaacagcta ctcaagggga ggtcaatgtg actggcgtga taccactgac aacaacacca      180

acaaaatctt attttgcaaa tctcaaagga acaaggacca gagggaaact atgcccggac      240

tgtctcaact gtacagatct ggatgtggcc ttgggcaggc caatgtgtgt ggggaccaca      300

ccttctgcta aagcttcaat actccatgag gtcagacctg ttacatccgg gtgctttcct      360

ataatgcacg acagaacaaa aatcaggcaa ctacccaatc ttctcagagg atatgaaaag      420

atcaggttat caacccaaaa cgttatcgat gcagaaaaag caccaggagg accctacaga      480

cttgccacct caggatcttg ccctaacgct accagtaaaa tcggattttt tgcaacaatg      540

gcttgggctg tcccaaagga caactacaaa aatgcaacga acccactaac agtggaagta      600

ccatacattt gtacagaagg ggaagaccaa attactgttt gggggttcca ttcggataac      660

aaaacccaaa tgaagagcct ctatggagac tcaaatcctc aaaagttcac ctcatctgct      720

aatggagtaa ccacgcatta tgtttctcag attggcgact tcccagatca aacagaagac      780

ggaggactac cacaaagcgg cagaattgtt gttgattaca tgatgcaaaa acctgggaaa      840

acaggaacaa ttgtctatca aaggggtgtt ttgttgcctc aaaaggtgtg gtgcgcgagt      900

ggcaggagca aagtaataaa agggtcattg cctttaattg gtgaagcaga ttgccttcat      960

gaagaatacg gtggattaaa caaaagcaag ccttactaca caggaaaaca tgcaaaagcc     1020

ataggaaatt gcccaatatg ggtaaaaaca cctttgaagc ttgccaatgg aaccaaatat     1080

agacctcctg gtggaggatg ggaaggaatg attgcaggtt ggcacggata cacatctcac     1140

ggagcacatg gagtggcagt ggcggcagac cttaagagta cacaagaagc tataaataag     1200

ataacaaaaa atctcaattc tttgagtgaa ctagaagtaa agaaccttca aagactaagt     1260

ggtgccatgg atgaactcca caacgaaata ctcgagctgg atgaaaaagt ggatgatctc     1320

agagctgaca ctataagctc acaaatagaa cttgcagtct tgctttccaa cgaaggaata     1380

ataaacagtg aagacgagca tctattggca cttgagagaa aactaaagaa aatgctgggt     1440

ccctctgctg tagacatagg aaacggatgc ttcgaaacca aacacaaatg caaccagacc     1500

tgcttagaca ggatagctgc tggcaccttt aatgcaggag aattttctct ccccactttt     1560

gattcattga acattactgc tgcatcttta aatgatgatg gattggataa ctaccagatt     1620

ttggcgatct attcaactgt cgccagttca ttggtactgg tagtctccct gggggcaatc     1680

agtttctgga tgtgctctaa tgggtctcta cagtgtagaa tatgtattta a              1731


<210>  41
<211>  576
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  PDI B/Phuket/3073/2013 G138A

<400>  41

Met Ala Lys Asn Val Ala Ile Phe Gly Leu Leu Phe Ser Leu Leu Val 
1               5                   10                  15      


Leu Val Pro Ser Gln Ile Phe Ala Asp Arg Ile Cys Thr Gly Ile Thr 
            20                  25                  30          


Ser Ser Asn Ser Pro His Val Val Lys Thr Ala Thr Gln Gly Glu Val 
        35                  40                  45              


Asn Val Thr Gly Val Ile Pro Leu Thr Thr Thr Pro Thr Lys Ser Tyr 
    50                  55                  60                  


Phe Ala Asn Leu Lys Gly Thr Arg Thr Arg Gly Lys Leu Cys Pro Asp 
65                  70                  75                  80  


Cys Leu Asn Cys Thr Asp Leu Asp Val Ala Leu Gly Arg Pro Met Cys 
                85                  90                  95      


Val Gly Thr Thr Pro Ser Ala Lys Ala Ser Ile Leu His Glu Val Arg 
            100                 105                 110         


Pro Val Thr Ser Gly Cys Phe Pro Ile Met His Asp Arg Thr Lys Ile 
        115                 120                 125             


Arg Gln Leu Pro Asn Leu Leu Arg Gly Tyr Glu Lys Ile Arg Leu Ser 
    130                 135                 140                 


Thr Gln Asn Val Ile Asp Ala Glu Lys Ala Pro Gly Gly Pro Tyr Arg 
145                 150                 155                 160 


Leu Ala Thr Ser Gly Ser Cys Pro Asn Ala Thr Ser Lys Ile Gly Phe 
                165                 170                 175     


Phe Ala Thr Met Ala Trp Ala Val Pro Lys Asp Asn Tyr Lys Asn Ala 
            180                 185                 190         


Thr Asn Pro Leu Thr Val Glu Val Pro Tyr Ile Cys Thr Glu Gly Glu 
        195                 200                 205             


Asp Gln Ile Thr Val Trp Gly Phe His Ser Asp Asn Lys Thr Gln Met 
    210                 215                 220                 


Lys Ser Leu Tyr Gly Asp Ser Asn Pro Gln Lys Phe Thr Ser Ser Ala 
225                 230                 235                 240 


Asn Gly Val Thr Thr His Tyr Val Ser Gln Ile Gly Asp Phe Pro Asp 
                245                 250                 255     


Gln Thr Glu Asp Gly Gly Leu Pro Gln Ser Gly Arg Ile Val Val Asp 
            260                 265                 270         


Tyr Met Met Gln Lys Pro Gly Lys Thr Gly Thr Ile Val Tyr Gln Arg 
        275                 280                 285             


Gly Val Leu Leu Pro Gln Lys Val Trp Cys Ala Ser Gly Arg Ser Lys 
    290                 295                 300                 


Val Ile Lys Gly Ser Leu Pro Leu Ile Gly Glu Ala Asp Cys Leu His 
305                 310                 315                 320 


Glu Glu Tyr Gly Gly Leu Asn Lys Ser Lys Pro Tyr Tyr Thr Gly Lys 
                325                 330                 335     


His Ala Lys Ala Ile Gly Asn Cys Pro Ile Trp Val Lys Thr Pro Leu 
            340                 345                 350         


Lys Leu Ala Asn Gly Thr Lys Tyr Arg Pro Pro Gly Gly Gly Trp Glu 
        355                 360                 365             


Gly Met Ile Ala Gly Trp His Gly Tyr Thr Ser His Gly Ala His Gly 
    370                 375                 380                 


Val Ala Val Ala Ala Asp Leu Lys Ser Thr Gln Glu Ala Ile Asn Lys 
385                 390                 395                 400 


Ile Thr Lys Asn Leu Asn Ser Leu Ser Glu Leu Glu Val Lys Asn Leu 
                405                 410                 415     


Gln Arg Leu Ser Gly Ala Met Asp Glu Leu His Asn Glu Ile Leu Glu 
            420                 425                 430         


Leu Asp Glu Lys Val Asp Asp Leu Arg Ala Asp Thr Ile Ser Ser Gln 
        435                 440                 445             


Ile Glu Leu Ala Val Leu Leu Ser Asn Glu Gly Ile Ile Asn Ser Glu 
    450                 455                 460                 


Asp Glu His Leu Leu Ala Leu Glu Arg Lys Leu Lys Lys Met Leu Gly 
465                 470                 475                 480 


Pro Ser Ala Val Asp Ile Gly Asn Gly Cys Phe Glu Thr Lys His Lys 
                485                 490                 495     


Cys Asn Gln Thr Cys Leu Asp Arg Ile Ala Ala Gly Thr Phe Asn Ala 
            500                 505                 510         


Gly Glu Phe Ser Leu Pro Thr Phe Asp Ser Leu Asn Ile Thr Ala Ala 
        515                 520                 525             


Ser Leu Asn Asp Asp Gly Leu Asp Asn Tyr Gln Ile Leu Ala Ile Tyr 
    530                 535                 540                 


Ser Thr Val Ala Ser Ser Leu Val Leu Val Val Ser Leu Gly Ala Ile 
545                 550                 555                 560 


Ser Phe Trp Met Cys Ser Asn Gly Ser Leu Gln Cys Arg Ile Cys Ile 
                565                 570                 575     


<210>  42
<211>  49
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer B_Phuket(L203A).c

<400>  42
cccaaatgaa gagcgcctat ggagactcaa atcctcaaaa gttcacctc                   49


<210>  43
<211>  48
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer B_Phuket(L203A).r

<400>  43
gatttgagtc tccataggcg ctcttcattt gggttttgtt atccgaat                    48


<210>  44
<211>  1731
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PDI-B Phu (L203A, Prl-)

<400>  44
atggcgaaaa acgttgcgat tttcggctta ttgttttctc ttcttgtgtt ggttccttct       60

cagatcttcg cggatcgaat ctgcactggg ataacatctt caaactcacc tcatgtggtc      120

aaaacagcta ctcaagggga ggtcaatgtg actggcgtga taccactgac aacaacacca      180

acaaaatctt attttgcaaa tctcaaagga acaaggacca gagggaaact atgcccggac      240

tgtctcaact gtacagatct ggatgtggcc ttgggcaggc caatgtgtgt ggggaccaca      300

ccttctgcta aagcttcaat actccatgag gtcagacctg ttacatccgg gtgctttcct      360

ataatgcacg acagaacaaa aatcaggcaa ctacccaatc ttctcagagg atatgaaaag      420

atcaggttat caacccaaaa cgttatcgat gcagaaaaag caccaggagg accctacaga      480

cttggaacct caggatcttg ccctaacgct accagtaaaa tcggattttt tgcaacaatg      540

gcttgggctg tcccaaagga caactacaaa aatgcaacga acccactaac agtggaagta      600

ccatacattt gtacagaagg ggaagaccaa attactgttt gggggttcca ttcggataac      660

aaaacccaaa tgaagagcgc ctatggagac tcaaatcctc aaaagttcac ctcatctgct      720

aatggagtaa ccacgcatta tgtttctcag attggcgact tcccagatca aacagaagac      780

ggaggactac cacaaagcgg cagaattgtt gttgattaca tgatgcaaaa acctgggaaa      840

acaggaacaa ttgtctatca aaggggtgtt ttgttgcctc aaaaggtgtg gtgcgcgagt      900

ggcaggagca aagtaataaa agggtcattg cctttaattg gtgaagcaga ttgccttcat      960

gaagaatacg gtggattaaa caaaagcaag ccttactaca caggaaaaca tgcaaaagcc     1020

ataggaaatt gcccaatatg ggtaaaaaca cctttgaagc ttgccaatgg aaccaaatat     1080

agacctcctg gtggaggatg ggaaggaatg attgcaggtt ggcacggata cacatctcac     1140

ggagcacatg gagtggcagt ggcggcagac cttaagagta cacaagaagc tataaataag     1200

ataacaaaaa atctcaattc tttgagtgaa ctagaagtaa agaaccttca aagactaagt     1260

ggtgccatgg atgaactcca caacgaaata ctcgagctgg atgaaaaagt ggatgatctc     1320

agagctgaca ctataagctc acaaatagaa cttgcagtct tgctttccaa cgaaggaata     1380

ataaacagtg aagacgagca tctattggca cttgagagaa aactaaagaa aatgctgggt     1440

ccctctgctg tagacatagg aaacggatgc ttcgaaacca aacacaaatg caaccagacc     1500

tgcttagaca ggatagctgc tggcaccttt aatgcaggag aattttctct ccccactttt     1560

gattcattga acattactgc tgcatcttta aatgatgatg gattggataa ctaccagatt     1620

ttggcgatct attcaactgt cgccagttca ttggtactgg tagtctccct gggggcaatc     1680

agtttctgga tgtgctctaa tgggtctcta cagtgtagaa tatgtattta a              1731


<210>  45
<211>  576
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  PDI-B Phu (L203A, Prl-)

<400>  45

Met Ala Lys Asn Val Ala Ile Phe Gly Leu Leu Phe Ser Leu Leu Val 
1               5                   10                  15      


Leu Val Pro Ser Gln Ile Phe Ala Asp Arg Ile Cys Thr Gly Ile Thr 
            20                  25                  30          


Ser Ser Asn Ser Pro His Val Val Lys Thr Ala Thr Gln Gly Glu Val 
        35                  40                  45              


Asn Val Thr Gly Val Ile Pro Leu Thr Thr Thr Pro Thr Lys Ser Tyr 
    50                  55                  60                  


Phe Ala Asn Leu Lys Gly Thr Arg Thr Arg Gly Lys Leu Cys Pro Asp 
65                  70                  75                  80  


Cys Leu Asn Cys Thr Asp Leu Asp Val Ala Leu Gly Arg Pro Met Cys 
                85                  90                  95      


Val Gly Thr Thr Pro Ser Ala Lys Ala Ser Ile Leu His Glu Val Arg 
            100                 105                 110         


Pro Val Thr Ser Gly Cys Phe Pro Ile Met His Asp Arg Thr Lys Ile 
        115                 120                 125             


Arg Gln Leu Pro Asn Leu Leu Arg Gly Tyr Glu Lys Ile Arg Leu Ser 
    130                 135                 140                 


Thr Gln Asn Val Ile Asp Ala Glu Lys Ala Pro Gly Gly Pro Tyr Arg 
145                 150                 155                 160 


Leu Gly Thr Ser Gly Ser Cys Pro Asn Ala Thr Ser Lys Ile Gly Phe 
                165                 170                 175     


Phe Ala Thr Met Ala Trp Ala Val Pro Lys Asp Asn Tyr Lys Asn Ala 
            180                 185                 190         


Thr Asn Pro Leu Thr Val Glu Val Pro Tyr Ile Cys Thr Glu Gly Glu 
        195                 200                 205             


Asp Gln Ile Thr Val Trp Gly Phe His Ser Asp Asn Lys Thr Gln Met 
    210                 215                 220                 


Lys Ser Ala Tyr Gly Asp Ser Asn Pro Gln Lys Phe Thr Ser Ser Ala 
225                 230                 235                 240 


Asn Gly Val Thr Thr His Tyr Val Ser Gln Ile Gly Asp Phe Pro Asp 
                245                 250                 255     


Gln Thr Glu Asp Gly Gly Leu Pro Gln Ser Gly Arg Ile Val Val Asp 
            260                 265                 270         


Tyr Met Met Gln Lys Pro Gly Lys Thr Gly Thr Ile Val Tyr Gln Arg 
        275                 280                 285             


Gly Val Leu Leu Pro Gln Lys Val Trp Cys Ala Ser Gly Arg Ser Lys 
    290                 295                 300                 


Val Ile Lys Gly Ser Leu Pro Leu Ile Gly Glu Ala Asp Cys Leu His 
305                 310                 315                 320 


Glu Glu Tyr Gly Gly Leu Asn Lys Ser Lys Pro Tyr Tyr Thr Gly Lys 
                325                 330                 335     


His Ala Lys Ala Ile Gly Asn Cys Pro Ile Trp Val Lys Thr Pro Leu 
            340                 345                 350         


Lys Leu Ala Asn Gly Thr Lys Tyr Arg Pro Pro Gly Gly Gly Trp Glu 
        355                 360                 365             


Gly Met Ile Ala Gly Trp His Gly Tyr Thr Ser His Gly Ala His Gly 
    370                 375                 380                 


Val Ala Val Ala Ala Asp Leu Lys Ser Thr Gln Glu Ala Ile Asn Lys 
385                 390                 395                 400 


Ile Thr Lys Asn Leu Asn Ser Leu Ser Glu Leu Glu Val Lys Asn Leu 
                405                 410                 415     


Gln Arg Leu Ser Gly Ala Met Asp Glu Leu His Asn Glu Ile Leu Glu 
            420                 425                 430         


Leu Asp Glu Lys Val Asp Asp Leu Arg Ala Asp Thr Ile Ser Ser Gln 
        435                 440                 445             


Ile Glu Leu Ala Val Leu Leu Ser Asn Glu Gly Ile Ile Asn Ser Glu 
    450                 455                 460                 


Asp Glu His Leu Leu Ala Leu Glu Arg Lys Leu Lys Lys Met Leu Gly 
465                 470                 475                 480 


Pro Ser Ala Val Asp Ile Gly Asn Gly Cys Phe Glu Thr Lys His Lys 
                485                 490                 495     


Cys Asn Gln Thr Cys Leu Asp Arg Ile Ala Ala Gly Thr Phe Asn Ala 
            500                 505                 510         


Gly Glu Phe Ser Leu Pro Thr Phe Asp Ser Leu Asn Ile Thr Ala Ala 
        515                 520                 525             


Ser Leu Asn Asp Asp Gly Leu Asp Asn Tyr Gln Ile Leu Ala Ile Tyr 
    530                 535                 540                 


Ser Thr Val Ala Ser Ser Leu Val Leu Val Val Ser Leu Gly Ala Ile 
545                 550                 555                 560 


Ser Phe Trp Met Cys Ser Asn Gly Ser Leu Gln Cys Arg Ile Cys Ile 
                565                 570                 575     


<210>  46
<211>  49
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer B_Phuket(D195G).c

<400>  46
gggggttcca ttcgggcaac aaaacccaaa tgaagagcct ctatggaga                   49


<210>  47
<211>  48
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer B_Phuket(D195G).r

<400>  47
tcatttgggt tttgttgccc gaatggaacc cccaaacagt aatttggt                    48


<210>  48
<211>  1731
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PDI-B Phu (D195G, Prl-)

<400>  48
atggcgaaaa acgttgcgat tttcggctta ttgttttctc ttcttgtgtt ggttccttct       60

cagatcttcg cggatcgaat ctgcactggg ataacatctt caaactcacc tcatgtggtc      120

aaaacagcta ctcaagggga ggtcaatgtg actggcgtga taccactgac aacaacacca      180

acaaaatctt attttgcaaa tctcaaagga acaaggacca gagggaaact atgcccggac      240

tgtctcaact gtacagatct ggatgtggcc ttgggcaggc caatgtgtgt ggggaccaca      300

ccttctgcta aagcttcaat actccatgag gtcagacctg ttacatccgg gtgctttcct      360

ataatgcacg acagaacaaa aatcaggcaa ctacccaatc ttctcagagg atatgaaaag      420

atcaggttat caacccaaaa cgttatcgat gcagaaaaag caccaggagg accctacaga      480

cttggaacct caggatcttg ccctaacgct accagtaaaa tcggattttt tgcaacaatg      540

gcttgggctg tcccaaagga caactacaaa aatgcaacga acccactaac agtggaagta      600

ccatacattt gtacagaagg ggaagaccaa attactgttt gggggttcca ttcgggcaac      660

aaaacccaaa tgaagagcct ctatggagac tcaaatcctc aaaagttcac ctcatctgct      720

aatggagtaa ccacgcatta tgtttctcag attggcgact tcccagatca aacagaagac      780

ggaggactac cacaaagcgg cagaattgtt gttgattaca tgatgcaaaa acctgggaaa      840

acaggaacaa ttgtctatca aaggggtgtt ttgttgcctc aaaaggtgtg gtgcgcgagt      900

ggcaggagca aagtaataaa agggtcattg cctttaattg gtgaagcaga ttgccttcat      960

gaagaatacg gtggattaaa caaaagcaag ccttactaca caggaaaaca tgcaaaagcc     1020

ataggaaatt gcccaatatg ggtaaaaaca cctttgaagc ttgccaatgg aaccaaatat     1080

agacctcctg gtggaggatg ggaaggaatg attgcaggtt ggcacggata cacatctcac     1140

ggagcacatg gagtggcagt ggcggcagac cttaagagta cacaagaagc tataaataag     1200

ataacaaaaa atctcaattc tttgagtgaa ctagaagtaa agaaccttca aagactaagt     1260

ggtgccatgg atgaactcca caacgaaata ctcgagctgg atgaaaaagt ggatgatctc     1320

agagctgaca ctataagctc acaaatagaa cttgcagtct tgctttccaa cgaaggaata     1380

ataaacagtg aagacgagca tctattggca cttgagagaa aactaaagaa aatgctgggt     1440

ccctctgctg tagacatagg aaacggatgc ttcgaaacca aacacaaatg caaccagacc     1500

tgcttagaca ggatagctgc tggcaccttt aatgcaggag aattttctct ccccactttt     1560

gattcattga acattactgc tgcatcttta aatgatgatg gattggataa ctaccagatt     1620

ttggcgatct attcaactgt cgccagttca ttggtactgg tagtctccct gggggcaatc     1680

agtttctgga tgtgctctaa tgggtctcta cagtgtagaa tatgtattta a              1731


<210>  49
<211>  576
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  PDI-B Phu (D195G, Prl-)

<400>  49

Met Ala Lys Asn Val Ala Ile Phe Gly Leu Leu Phe Ser Leu Leu Val 
1               5                   10                  15      


Leu Val Pro Ser Gln Ile Phe Ala Asp Arg Ile Cys Thr Gly Ile Thr 
            20                  25                  30          


Ser Ser Asn Ser Pro His Val Val Lys Thr Ala Thr Gln Gly Glu Val 
        35                  40                  45              


Asn Val Thr Gly Val Ile Pro Leu Thr Thr Thr Pro Thr Lys Ser Tyr 
    50                  55                  60                  


Phe Ala Asn Leu Lys Gly Thr Arg Thr Arg Gly Lys Leu Cys Pro Asp 
65                  70                  75                  80  


Cys Leu Asn Cys Thr Asp Leu Asp Val Ala Leu Gly Arg Pro Met Cys 
                85                  90                  95      


Val Gly Thr Thr Pro Ser Ala Lys Ala Ser Ile Leu His Glu Val Arg 
            100                 105                 110         


Pro Val Thr Ser Gly Cys Phe Pro Ile Met His Asp Arg Thr Lys Ile 
        115                 120                 125             


Arg Gln Leu Pro Asn Leu Leu Arg Gly Tyr Glu Lys Ile Arg Leu Ser 
    130                 135                 140                 


Thr Gln Asn Val Ile Asp Ala Glu Lys Ala Pro Gly Gly Pro Tyr Arg 
145                 150                 155                 160 


Leu Gly Thr Ser Gly Ser Cys Pro Asn Ala Thr Ser Lys Ile Gly Phe 
                165                 170                 175     


Phe Ala Thr Met Ala Trp Ala Val Pro Lys Asp Asn Tyr Lys Asn Ala 
            180                 185                 190         


Thr Asn Pro Leu Thr Val Glu Val Pro Tyr Ile Cys Thr Glu Gly Glu 
        195                 200                 205             


Asp Gln Ile Thr Val Trp Gly Phe His Ser Gly Asn Lys Thr Gln Met 
    210                 215                 220                 


Lys Ser Leu Tyr Gly Asp Ser Asn Pro Gln Lys Phe Thr Ser Ser Ala 
225                 230                 235                 240 


Asn Gly Val Thr Thr His Tyr Val Ser Gln Ile Gly Asp Phe Pro Asp 
                245                 250                 255     


Gln Thr Glu Asp Gly Gly Leu Pro Gln Ser Gly Arg Ile Val Val Asp 
            260                 265                 270         


Tyr Met Met Gln Lys Pro Gly Lys Thr Gly Thr Ile Val Tyr Gln Arg 
        275                 280                 285             


Gly Val Leu Leu Pro Gln Lys Val Trp Cys Ala Ser Gly Arg Ser Lys 
    290                 295                 300                 


Val Ile Lys Gly Ser Leu Pro Leu Ile Gly Glu Ala Asp Cys Leu His 
305                 310                 315                 320 


Glu Glu Tyr Gly Gly Leu Asn Lys Ser Lys Pro Tyr Tyr Thr Gly Lys 
                325                 330                 335     


His Ala Lys Ala Ile Gly Asn Cys Pro Ile Trp Val Lys Thr Pro Leu 
            340                 345                 350         


Lys Leu Ala Asn Gly Thr Lys Tyr Arg Pro Pro Gly Gly Gly Trp Glu 
        355                 360                 365             


Gly Met Ile Ala Gly Trp His Gly Tyr Thr Ser His Gly Ala His Gly 
    370                 375                 380                 


Val Ala Val Ala Ala Asp Leu Lys Ser Thr Gln Glu Ala Ile Asn Lys 
385                 390                 395                 400 


Ile Thr Lys Asn Leu Asn Ser Leu Ser Glu Leu Glu Val Lys Asn Leu 
                405                 410                 415     


Gln Arg Leu Ser Gly Ala Met Asp Glu Leu His Asn Glu Ile Leu Glu 
            420                 425                 430         


Leu Asp Glu Lys Val Asp Asp Leu Arg Ala Asp Thr Ile Ser Ser Gln 
        435                 440                 445             


Ile Glu Leu Ala Val Leu Leu Ser Asn Glu Gly Ile Ile Asn Ser Glu 
    450                 455                 460                 


Asp Glu His Leu Leu Ala Leu Glu Arg Lys Leu Lys Lys Met Leu Gly 
465                 470                 475                 480 


Pro Ser Ala Val Asp Ile Gly Asn Gly Cys Phe Glu Thr Lys His Lys 
                485                 490                 495     


Cys Asn Gln Thr Cys Leu Asp Arg Ile Ala Ala Gly Thr Phe Asn Ala 
            500                 505                 510         


Gly Glu Phe Ser Leu Pro Thr Phe Asp Ser Leu Asn Ile Thr Ala Ala 
        515                 520                 525             


Ser Leu Asn Asp Asp Gly Leu Asp Asn Tyr Gln Ile Leu Ala Ile Tyr 
    530                 535                 540                 


Ser Thr Val Ala Ser Ser Leu Val Leu Val Val Ser Leu Gly Ala Ile 
545                 550                 555                 560 


Ser Phe Trp Met Cys Ser Asn Gly Ser Leu Gln Cys Arg Ile Cys Ile 
                565                 570                 575     


<210>  50
<211>  49
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer B_Phuket(L203W).c

<400>  50
cccaaatgaa gagctggtat ggagactcaa atcctcaaaa gttcacctc                   49


<210>  51
<211>  48
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer B_Phuket(L203W).r

<400>  51
gatttgagtc tccataccag ctcttcattt gggttttgtt atccgaat                    48


<210>  52
<211>  1731
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PDI-B Phu (L203W, Prl-)

<400>  52
atggcgaaaa acgttgcgat tttcggctta ttgttttctc ttcttgtgtt ggttccttct       60

cagatcttcg cggatcgaat ctgcactggg ataacatctt caaactcacc tcatgtggtc      120

aaaacagcta ctcaagggga ggtcaatgtg actggcgtga taccactgac aacaacacca      180

acaaaatctt attttgcaaa tctcaaagga acaaggacca gagggaaact atgcccggac      240

tgtctcaact gtacagatct ggatgtggcc ttgggcaggc caatgtgtgt ggggaccaca      300

ccttctgcta aagcttcaat actccatgag gtcagacctg ttacatccgg gtgctttcct      360

ataatgcacg acagaacaaa aatcaggcaa ctacccaatc ttctcagagg atatgaaaag      420

atcaggttat caacccaaaa cgttatcgat gcagaaaaag caccaggagg accctacaga      480

cttggaacct caggatcttg ccctaacgct accagtaaaa tcggattttt tgcaacaatg      540

gcttgggctg tcccaaagga caactacaaa aatgcaacga acccactaac agtggaagta      600

ccatacattt gtacagaagg ggaagaccaa attactgttt gggggttcca ttcggataac      660

aaaacccaaa tgaagagctg gtatggagac tcaaatcctc aaaagttcac ctcatctgct      720

aatggagtaa ccacgcatta tgtttctcag attggcgact tcccagatca aacagaagac      780

ggaggactac cacaaagcgg cagaattgtt gttgattaca tgatgcaaaa acctgggaaa      840

acaggaacaa ttgtctatca aaggggtgtt ttgttgcctc aaaaggtgtg gtgcgcgagt      900

ggcaggagca aagtaataaa agggtcattg cctttaattg gtgaagcaga ttgccttcat      960

gaagaatacg gtggattaaa caaaagcaag ccttactaca caggaaaaca tgcaaaagcc     1020

ataggaaatt gcccaatatg ggtaaaaaca cctttgaagc ttgccaatgg aaccaaatat     1080

agacctcctg gtggaggatg ggaaggaatg attgcaggtt ggcacggata cacatctcac     1140

ggagcacatg gagtggcagt ggcggcagac cttaagagta cacaagaagc tataaataag     1200

ataacaaaaa atctcaattc tttgagtgaa ctagaagtaa agaaccttca aagactaagt     1260

ggtgccatgg atgaactcca caacgaaata ctcgagctgg atgaaaaagt ggatgatctc     1320

agagctgaca ctataagctc acaaatagaa cttgcagtct tgctttccaa cgaaggaata     1380

ataaacagtg aagacgagca tctattggca cttgagagaa aactaaagaa aatgctgggt     1440

ccctctgctg tagacatagg aaacggatgc ttcgaaacca aacacaaatg caaccagacc     1500

tgcttagaca ggatagctgc tggcaccttt aatgcaggag aattttctct ccccactttt     1560

gattcattga acattactgc tgcatcttta aatgatgatg gattggataa ctaccagatt     1620

ttggcgatct attcaactgt cgccagttca ttggtactgg tagtctccct gggggcaatc     1680

agtttctgga tgtgctctaa tgggtctcta cagtgtagaa tatgtattta a              1731


<210>  53
<211>  576
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  PDI-B Phu (L203W, Prl-)

<400>  53

Met Ala Lys Asn Val Ala Ile Phe Gly Leu Leu Phe Ser Leu Leu Val 
1               5                   10                  15      


Leu Val Pro Ser Gln Ile Phe Ala Asp Arg Ile Cys Thr Gly Ile Thr 
            20                  25                  30          


Ser Ser Asn Ser Pro His Val Val Lys Thr Ala Thr Gln Gly Glu Val 
        35                  40                  45              


Asn Val Thr Gly Val Ile Pro Leu Thr Thr Thr Pro Thr Lys Ser Tyr 
    50                  55                  60                  


Phe Ala Asn Leu Lys Gly Thr Arg Thr Arg Gly Lys Leu Cys Pro Asp 
65                  70                  75                  80  


Cys Leu Asn Cys Thr Asp Leu Asp Val Ala Leu Gly Arg Pro Met Cys 
                85                  90                  95      


Val Gly Thr Thr Pro Ser Ala Lys Ala Ser Ile Leu His Glu Val Arg 
            100                 105                 110         


Pro Val Thr Ser Gly Cys Phe Pro Ile Met His Asp Arg Thr Lys Ile 
        115                 120                 125             


Arg Gln Leu Pro Asn Leu Leu Arg Gly Tyr Glu Lys Ile Arg Leu Ser 
    130                 135                 140                 


Thr Gln Asn Val Ile Asp Ala Glu Lys Ala Pro Gly Gly Pro Tyr Arg 
145                 150                 155                 160 


Leu Gly Thr Ser Gly Ser Cys Pro Asn Ala Thr Ser Lys Ile Gly Phe 
                165                 170                 175     


Phe Ala Thr Met Ala Trp Ala Val Pro Lys Asp Asn Tyr Lys Asn Ala 
            180                 185                 190         


Thr Asn Pro Leu Thr Val Glu Val Pro Tyr Ile Cys Thr Glu Gly Glu 
        195                 200                 205             


Asp Gln Ile Thr Val Trp Gly Phe His Ser Asp Asn Lys Thr Gln Met 
    210                 215                 220                 


Lys Ser Trp Tyr Gly Asp Ser Asn Pro Gln Lys Phe Thr Ser Ser Ala 
225                 230                 235                 240 


Asn Gly Val Thr Thr His Tyr Val Ser Gln Ile Gly Asp Phe Pro Asp 
                245                 250                 255     


Gln Thr Glu Asp Gly Gly Leu Pro Gln Ser Gly Arg Ile Val Val Asp 
            260                 265                 270         


Tyr Met Met Gln Lys Pro Gly Lys Thr Gly Thr Ile Val Tyr Gln Arg 
        275                 280                 285             


Gly Val Leu Leu Pro Gln Lys Val Trp Cys Ala Ser Gly Arg Ser Lys 
    290                 295                 300                 


Val Ile Lys Gly Ser Leu Pro Leu Ile Gly Glu Ala Asp Cys Leu His 
305                 310                 315                 320 


Glu Glu Tyr Gly Gly Leu Asn Lys Ser Lys Pro Tyr Tyr Thr Gly Lys 
                325                 330                 335     


His Ala Lys Ala Ile Gly Asn Cys Pro Ile Trp Val Lys Thr Pro Leu 
            340                 345                 350         


Lys Leu Ala Asn Gly Thr Lys Tyr Arg Pro Pro Gly Gly Gly Trp Glu 
        355                 360                 365             


Gly Met Ile Ala Gly Trp His Gly Tyr Thr Ser His Gly Ala His Gly 
    370                 375                 380                 


Val Ala Val Ala Ala Asp Leu Lys Ser Thr Gln Glu Ala Ile Asn Lys 
385                 390                 395                 400 


Ile Thr Lys Asn Leu Asn Ser Leu Ser Glu Leu Glu Val Lys Asn Leu 
                405                 410                 415     


Gln Arg Leu Ser Gly Ala Met Asp Glu Leu His Asn Glu Ile Leu Glu 
            420                 425                 430         


Leu Asp Glu Lys Val Asp Asp Leu Arg Ala Asp Thr Ile Ser Ser Gln 
        435                 440                 445             


Ile Glu Leu Ala Val Leu Leu Ser Asn Glu Gly Ile Ile Asn Ser Glu 
    450                 455                 460                 


Asp Glu His Leu Leu Ala Leu Glu Arg Lys Leu Lys Lys Met Leu Gly 
465                 470                 475                 480 


Pro Ser Ala Val Asp Ile Gly Asn Gly Cys Phe Glu Thr Lys His Lys 
                485                 490                 495     


Cys Asn Gln Thr Cys Leu Asp Arg Ile Ala Ala Gly Thr Phe Asn Ala 
            500                 505                 510         


Gly Glu Phe Ser Leu Pro Thr Phe Asp Ser Leu Asn Ile Thr Ala Ala 
        515                 520                 525             


Ser Leu Asn Asp Asp Gly Leu Asp Asn Tyr Gln Ile Leu Ala Ile Tyr 
    530                 535                 540                 


Ser Thr Val Ala Ser Ser Leu Val Leu Val Val Ser Leu Gly Ala Ile 
545                 550                 555                 560 


Ser Phe Trp Met Cys Ser Asn Gly Ser Leu Gln Cys Arg Ile Cys Ile 
                565                 570                 575     


<210>  54
<211>  6443
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Cloning vector 2530 from left to right T-DNA

<400>  54
tggcaggata tattgtggtg taaacaaatt gacgcttaga caacttaata acacattgcg       60

gacgttttta atgtactgaa ttaacgccga atcccgggct ggtatattta tatgttgtca      120

aataactcaa aaaccataaa agtttaagtt agcaagtgtg tacattttta cttgaacaaa      180

aatattcacc tactactgtt ataaatcatt attaaacatt agagtaaaga aatatggatg      240

ataagaacaa gagtagtgat attttgacaa caattttgtt gcaacatttg agaaaatttt      300

gttgttctct cttttcattg gtcaaaaaca atagagagag aaaaaggaag agggagaata      360

aaaacataat gtgagtatga gagagaaagt tgtacaaaag ttgtaccaaa atagttgtac      420

aaatatcatt gaggaatttg acaaaagcta cacaaataag ggttaattgc tgtaaataaa      480

taaggatgac gcattagaga gatgtaccat tagagaattt ttggcaagtc attaaaaaga      540

aagaataaat tatttttaaa attaaaagtt gagtcatttg attaaacatg tgattattta      600

atgaattgat gaaagagttg gattaaagtt gtattagtaa ttagaatttg gtgtcaaatt      660

taatttgaca tttgatcttt tcctatatat tgccccatag agtcagttaa ctcattttta      720

tatttcatag atcaaataag agaaataacg gtatattaat ccctccaaaa aaaaaaaacg      780

gtatatttac taaaaaatct aagccacgta ggaggataac aggatccccg taggaggata      840

acatccaatc caaccaatca caacaatcct gatgagataa cccactttaa gcccacgcat      900

ctgtggcaca tctacattat ctaaatcaca cattcttcca cacatctgag ccacacaaaa      960

accaatccac atctttatca cccattctat aaaaaatcac actttgtgag tctacacttt     1020

gattcccttc aaacacatac aaagagaaga gactaattaa ttaattaatc atcttgagag     1080

aaaatggaac gagctataca aggaaacgac gctagggaac aagctaacag tgaacgttgg     1140

gatggaggat caggaggtac cacttctccc ttcaaacttc ctgacgaaag tccgagttgg     1200

actgagtggc ggctacataa cgatgagacg aattcgaatc aagataatcc ccttggtttc     1260

aaggaaagct ggggtttcgg gaaagttgta tttaagagat atctcagata cgacaggacg     1320

gaagcttcac tgcacagagt ccttggatct tggacgggag attcggttaa ctatgcagca     1380

tctcgatttt tcggtttcga ccagatcgga tgtacctata gtattcggtt tcgaggagtt     1440

agtatcaccg tttctggagg gtcgcgaact cttcagcatc tctgtgagat ggcaattcgg     1500

tctaagcaag aactgctaca gcttgcccca atcgaagtgg aaagtaatgt atcaagagga     1560

tgccctgaag gtactcaaac cttcgaaaaa gaaagcgagt aagttaaaat gcttcttcgt     1620

ctcctattta taatatggtt tgttattgtt aattttgttc ttgtagaaga gcttaattaa     1680

tcgttgttgt tatgaaatac tatttgtatg agatgaactg gtgtaatgta attcatttac     1740

ataagtggag tcagaatcag aatgtttcct ccataactaa ctagacatga agacctgccg     1800

cgtacaattg tcttatattt gaacaactaa aattgaacat cttttgccac aactttataa     1860

gtggttaata tagctcaaat atatggtcaa gttcaataga ttaataatgg aaatatcagt     1920

tatcgaaatt cattaacaat caacttaacg ttattaacta ctaattttat atcatcccct     1980

ttgataaatg atagtacacc aattaggaag gagcatgctc gcctaggaga ttgtcgtttc     2040

ccgccttcag tttgcaagct gctctagccg tgtagccaat acgcaaaccg cctctccccg     2100

cgcgttggga attactagcg cgtgtcgaca cgcgtggcgc gccctggtat atttatatgt     2160

tgtcaaataa ctcaaaaacc ataaaagttt aagttagcaa gtgtgtacat ttttacttga     2220

acaaaaatat tcacctacta ctgttataaa tcattattaa acattagagt aaagaaatat     2280

ggatgataag aacaagagta gtgatatttt gacaacaatt ttgttgcaac atttgagaaa     2340

attttgttgt tctctctttt cattggtcaa aaacaataga gagagaaaaa ggaagaggga     2400

gaataaaaac ataatgtgag tatgagagag aaagttgtac aaaagttgta ccaaaatagt     2460

tgtacaaata tcattgagga atttgacaaa agctacacaa ataagggtta attgctgtaa     2520

ataaataagg atgacgcatt agagagatgt accattagag aatttttggc aagtcattaa     2580

aaagaaagaa taaattattt ttaaaattaa aagttgagtc atttgattaa acatgtgatt     2640

atttaatgaa ttgatgaaag agttggatta aagttgtatt agtaattaga atttggtgtc     2700

aaatttaatt tgacatttga tcttttccta tatattgccc catagagtca gttaactcat     2760

ttttatattt catagatcaa ataagagaaa taacggtata ttaatccctc caaaaaaaaa     2820

aaacggtata tttactaaaa aatctaagcc acgtaggagg ataacaggat ccccgtagga     2880

ggataacatc caatccaacc aatcacaaca atcctgatga gataacccac tttaagccca     2940

cgcatctgtg gcacatctac attatctaaa tcacacattc ttccacacat ctgagccaca     3000

caaaaaccaa tccacatctt tatcacccat tctataaaaa atcacacttt gtgagtctac     3060

actttgattc ccttcaaaca catacaaaga gaagagacta attaattaat taatcatctt     3120

gagagaaaat gagtcttcta accgaggtcg aaacgcctat cagaaacgaa tgggggtgca     3180

gatgcaacga ttcaagtgat cctcttgttg ttgccgcaag tataattggg attgtgcacc     3240

tgatattgtg gattattgat cgcctttttt ccaaaagcat ttatcgtatc tttaaacacg     3300

gtttaaaaag agggccttct acggaaggag taccagagtc tatgagggaa gaatatcgag     3360

aggaacagca gaatgctgtg gatgctgacg atggtcattt tgtcagcata gagctggagt     3420

aagagctcta agttaaaatg cttcttcgtc tcctatttat aatatggttt gttattgtta     3480

attttgttct tgtagaagag cttaattaat cgttgttgtt atgaaatact atttgtatga     3540

gatgaactgg tgtaatgtaa ttcatttaca taagtggagt cagaatcaga atgtttcctc     3600

cataactaac tagacatgaa gacctgccgc gtacaattgt cttatatttg aacaactaaa     3660

attgaacatc ttttgccaca actttataag tggttaatat agctcaaata tatggtcaag     3720

ttcaatagat taataatgga aatatcagtt atcgaaattc attaacaatc aacttaacgt     3780

tattaactac taattttata tcatcccctt tgataaatga tagtacacca attaggaagg     3840

aactaggaga ttgtcgtttc ccgccttcag tttgcaagct gctctagccg tgtagccaat     3900

acgcaaaccg cctctccccg cgcgttggga attactagcg cgtgtcgaca agcttgcatg     3960

ccggtcaaca tggtggagca cgacacactt gtctactcca aaaatatcaa agatacagtc     4020

tcagaagacc aaagggcaat tgagactttt caacaaaggg taatatccgg aaacctcctc     4080

ggattccatt gcccagctat ctgtcacttt attgtgaaga tagtggaaaa ggaaggtggc     4140

tcctacaaat gccatcattg cgataaagga aaggccatcg ttgaagatgc ctctgccgac     4200

agtggtccca aagatggacc cccacccacg aggagcatcg tggaaaaaga agacgttcca     4260

accacgtctt caaagcaagt ggattgatgt gataacatgg tggagcacga cacacttgtc     4320

tactccaaaa atatcaaaga tacagtctca gaagaccaaa gggcaattga gacttttcaa     4380

caaagggtaa tatccggaaa cctcctcgga ttccattgcc cagctatctg tcactttatt     4440

gtgaagatag tggaaaagga aggtggctcc tacaaatgcc atcattgcga taaaggaaag     4500

gccatcgttg aagatgcctc tgccgacagt ggtcccaaag atggaccccc acccacgagg     4560

agcatcgtgg aaaaagaaga cgttccaacc acgtcttcaa agcaagtgga ttgatgtgat     4620

atctccactg acgtaaggga tgacgcacaa tcccactatc cttcgcaaga cccttcctct     4680

atataaggaa gttcatttca tttggagagg tattaaaatc ttaataggtt ttgataaaag     4740

cgaacgtggg gaaacccgaa ccaaaccttc ttctaaactc tctctcatct ctcttaaagc     4800

aaacttctct cttgtctttc ttgcgtgagc gatcttcaac gttgtcagat cgtgcttcgg     4860

caccagtaca atggcgaaaa acgttgcgat tttcggctta ttgttttctc ttcttgtgtt     4920

ggttccttct cagatcttcg cgacgtcact cctcagccaa aacgacaccc ccatctgtct     4980

atccactggc ccctggatct gctgcccaaa ctaactccat ggtgaccctg ggatgcctgg     5040

tcaagggcta tttccctgag ccagtgacag tgacctggaa ctctggatcc ctgtccagcg     5100

gtgtgcacac cttcccagct gtcctgcagt ctgacctcta cactctgagc agctcagtga     5160

ctgtcccctc cagcacctgg cccagcgaga ccgtcacctg caacgttgcc cacccggcca     5220

gcagcaccaa ggtggacaag aaaattgtgc ccagggattg tggttgtaag ccttgcatat     5280

gtacagtccc agaagtatca tctgtcttca tcttcccccc aaagcccaag gatgtgctca     5340

ccattactct gactcctaag gtcacgtgtg ttgtggtaga catcagcaag gatgatcccg     5400

aggtccagtt cagctggttt gtagatgatg tggaggtgca cacagctcag acgcaacccc     5460

gggaggagca gttcaacagc actttccgct cagtcagtga acttcccatc atgcaccagg     5520

actggctcaa tggcaaggag acgtccagat tttggcgatc tattcaactg tcgccagttc     5580

attggtactg gtagtctccc tgggggcaat cagtttctgg atgtgctcta atgggtctct     5640

acagtgtaga atatgtattt aaaggcctat tttctttagt ttgaatttac tgttattcgg     5700

tgtgcatttc tatgtttggt gagcggtttt ctgtgctcag agtgtgttta ttttatgtaa     5760

tttaatttct ttgtgagctc ctgtttagca ggtcgtccct tcagcaagga cacaaaaaga     5820

ttttaatttt attaaaaaaa aaaaaaaaaa agaccgggaa ttcgatatca agcttatcga     5880

cctgcagatc gttcaaacat ttggcaataa agtttcttaa gattgaatcc tgttgccggt     5940

cttgcgatga ttatcatata atttctgttg aattacgtta agcatgtaat aattaacatg     6000

taatgcatga cgttatttat gagatgggtt tttatgatta gagtcccgca attatacatt     6060

taatacgcga tagaaaacaa aatatagcgc gcaaactagg ataaattatc gcgcgcggtg     6120

tcatctatgt tactagatct ctagagtctc aagcttggcg cgcccacgtg actagtggca     6180

ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc     6240

cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc     6300

ccttcccaac agttgcgcag cctgaatggc gaatgctaga gcagcttgag cttggatcag     6360

attgtcgttt cccgccttca gtttaaacta tcagtgtttg acaggatata ttggcgggta     6420

aacctaagag aaaagagcgt tta                                             6443


<210>  55
<211>  3114
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Construct 2835 from 2X35S prom to NOS term

<400>  55
gtcaacatgg tggagcacga cacacttgtc tactccaaaa atatcaaaga tacagtctca       60

gaagaccaaa gggcaattga gacttttcaa caaagggtaa tatccggaaa cctcctcgga      120

ttccattgcc cagctatctg tcactttatt gtgaagatag tggaaaagga aggtggctcc      180

tacaaatgcc atcattgcga taaaggaaag gccatcgttg aagatgcctc tgccgacagt      240

ggtcccaaag atggaccccc acccacgagg agcatcgtgg aaaaagaaga cgttccaacc      300

acgtcttcaa agcaagtgga ttgatgtgat aacatggtgg agcacgacac acttgtctac      360

tccaaaaata tcaaagatac agtctcagaa gaccaaaggg caattgagac ttttcaacaa      420

agggtaatat ccggaaacct cctcggattc cattgcccag ctatctgtca ctttattgtg      480

aagatagtgg aaaaggaagg tggctcctac aaatgccatc attgcgataa aggaaaggcc      540

atcgttgaag atgcctctgc cgacagtggt cccaaagatg gacccccacc cacgaggagc      600

atcgtggaaa aagaagacgt tccaaccacg tcttcaaagc aagtggattg atgtgatatc      660

tccactgacg taagggatga cgcacaatcc cactatcctt cgcaagaccc ttcctctata      720

taaggaagtt catttcattt ggagaggtat taaaatctta ataggttttg ataaaagcga      780

acgtggggaa acccgaacca aaccttcttc taaactctct ctcatctctc ttaaagcaaa      840

cttctctctt gtctttcttg cgtgagcgat cttcaacgtt gtcagatcgt gcttcggcac      900

cagtacaatg gcgaaaaacg ttgcgatttt cggcttattg ttttctcttc ttgtgttggt      960

tccttctcag atcttcgcgg atcgaatctg cactgggata acatcttcaa actcacctca     1020

tgtggtcaaa acagctactc aaggggaggt caatgtgact ggcgtgatac cactgacaac     1080

aacaccaaca aaatcttatt ttgcaaatct caaaggaaca aggaccagag ggaaactatg     1140

cccggactgt ctcaactgta cagatctgga tgtggccttg ggcaggccaa tgtgtgtggg     1200

gaccacacct tctgctaaag cttcaatact ccatgaggtc agacctgtta catccgggtg     1260

ctttcctata atgcacgaca gaacaaaaat caggcaacta cccaatcttc tcagaggata     1320

tgaaaagatc aggttatcaa cccaaaacgt tatcgatgca gaaaaagcac caggaggacc     1380

ctacagactt ggaacctcag gatcttgccc taacgctacc agtaaaatcg gattttttgc     1440

aacaatggct tgggctgtcc caaaggacaa ctacaaaaat gcaacgaacc cactaacagt     1500

ggaagtacca tacatttgta cagaagggga agaccaaatt actgtttggg ggttccattc     1560

ggataacaaa acccaaatga agagcctcta tggagactca aatcctcaaa agttcacctc     1620

atctgctaat ggagtaacca cgcattatgt ttctcagatt ggcgacttcc cagatcaaac     1680

agaagacgga ggactaccac aaagcggcag aattgttgtt gattacatga tgcaaaaacc     1740

tgggaaaaca ggaacaattg tctatcaaag gggtgttttg ttgcctcaaa aggtgtggtg     1800

cgcgagtggc aggagcaaag taataaaagg gtcattgcct ttaattggtg aagcagattg     1860

ccttcatgaa gaatacggtg gattaaacaa aagcaagcct tactacacag gaaaacatgc     1920

aaaagccata ggaaattgcc caatatgggt aaaaacacct ttgaagcttg ccaatggaac     1980

caaatataga cctcctggtg gaggatggga aggaatgatt gcaggttggc acggatacac     2040

atctcacgga gcacatggag tggcagtggc ggcagacctt aagagtacac aagaagctat     2100

aaataagata acaaaaaatc tcaattcttt gagtgaacta gaagtaaaga accttcaaag     2160

actaagtggt gccatggatg aactccacaa cgaaatactc gagctggatg aaaaagtgga     2220

tgatctcaga gctgacacta taagctcaca aatagaactt gcagtcttgc tttccaacga     2280

aggaataata aacagtgaag acgagcatct attggcactt gagagaaaac taaagaaaat     2340

gctgggtccc tctgctgtag acataggaaa cggatgcttc gaaaccaaac acaaatgcaa     2400

ccagacctgc ttagacagga tagctgctgg cacctttaat gcaggagaat tttctctccc     2460

cacttttgat tcattgaaca ttactgctgc atctttaaat gatgatggat tggataacta     2520

ccagattttg gcgatctatt caactgtcgc cagttcattg gtactggtag tctccctggg     2580

ggcaatcagt ttctggatgt gctctaatgg gtctctacag tgtagaatat gtatttaaag     2640

gcctattttc tttagtttga atttactgtt attcggtgtg catttctatg tttggtgagc     2700

ggttttctgt gctcagagtg tgtttatttt atgtaattta atttctttgt gagctcctgt     2760

ttagcaggtc gtcccttcag caaggacaca aaaagatttt aattttatta aaaaaaaaaa     2820

aaaaaaagac cgggaattcg atatcaagct tatcgacctg cagatcgttc aaacatttgg     2880

caataaagtt tcttaagatt gaatcctgtt gccggtcttg cgatgattat catataattt     2940

ctgttgaatt acgttaagca tgtaataatt aacatgtaat gcatgacgtt atttatgaga     3000

tgggttttta tgattagagt cccgcaatta tacatttaat acgcgataga aaacaaaata     3060

tagcgcgcaa actaggataa attatcgcgc gcggtgtcat ctatgttact agat           3114


<210>  56
<211>  6389
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Cloning vector 4499 from left to right T-DNA

<400>  56
tggcaggata tattgtggtg taaacaaatt gacgcttaga caacttaata acacattgcg       60

gacgttttta atgtactgaa ttaacgccga atcccgggct ggtatattta tatgttgtca      120

aataactcaa aaaccataaa agtttaagtt agcaagtgtg tacattttta cttgaacaaa      180

aatattcacc tactactgtt ataaatcatt attaaacatt agagtaaaga aatatggatg      240

ataagaacaa gagtagtgat attttgacaa caattttgtt gcaacatttg agaaaatttt      300

gttgttctct cttttcattg gtcaaaaaca atagagagag aaaaaggaag agggagaata      360

aaaacataat gtgagtatga gagagaaagt tgtacaaaag ttgtaccaaa atagttgtac      420

aaatatcatt gaggaatttg acaaaagcta cacaaataag ggttaattgc tgtaaataaa      480

taaggatgac gcattagaga gatgtaccat tagagaattt ttggcaagtc attaaaaaga      540

aagaataaat tatttttaaa attaaaagtt gagtcatttg attaaacatg tgattattta      600

atgaattgat gaaagagttg gattaaagtt gtattagtaa ttagaatttg gtgtcaaatt      660

taatttgaca tttgatcttt tcctatatat tgccccatag agtcagttaa ctcattttta      720

tatttcatag atcaaataag agaaataacg gtatattaat ccctccaaaa aaaaaaaacg      780

gtatatttac taaaaaatct aagccacgta ggaggataac aggatccccg taggaggata      840

acatccaatc caaccaatca caacaatcct gatgagataa cccactttaa gcccacgcat      900

ctgtggcaca tctacattat ctaaatcaca cattcttcca cacatctgag ccacacaaaa      960

accaatccac atctttatca cccattctat aaaaaatcac actttgtgag tctacacttt     1020

gattcccttc aaacacatac aaagagaaga gactaattaa ttaattaatc atcttgagag     1080

aaaatggaac gagctataca aggaaacgac gctagggaac aagctaacag tgaacgttgg     1140

gatggaggat caggaggtac cacttctccc ttcaaacttc ctgacgaaag tccgagttgg     1200

actgagtggc ggctacataa cgatgagacg aattcgaatc aagataatcc ccttggtttc     1260

aaggaaagct ggggtttcgg gaaagttgta tttaagagat atctcagata cgacaggacg     1320

gaagcttcac tgcacagagt ccttggatct tggacgggag attcggttaa ctatgcagca     1380

tctcgatttt tcggtttcga ccagatcgga tgtacctata gtattcggtt tcgaggagtt     1440

agtatcaccg tttctggagg gtcgcgaact cttcagcatc tctgtgagat ggcaattcgg     1500

tctaagcaag aactgctaca gcttgcccca atcgaagtgg aaagtaatgt atcaagagga     1560

tgccctgaag gtactcaaac cttcgaaaaa gaaagcgagt aagttaaaat gcttcttcgt     1620

ctcctattta taatatggtt tgttattgtt aattttgttc ttgtagaaga gcttaattaa     1680

tcgttgttgt tatgaaatac tatttgtatg agatgaactg gtgtaatgta attcatttac     1740

ataagtggag tcagaatcag aatgtttcct ccataactaa ctagacatga agacctgccg     1800

cgtacaattg tcttatattt gaacaactaa aattgaacat cttttgccac aactttataa     1860

gtggttaata tagctcaaat atatggtcaa gttcaataga ttaataatgg aaatatcagt     1920

tatcgaaatt cattaacaat caacttaacg ttattaacta ctaattttat atcatcccct     1980

ttgataaatg atagtacacc aattaggaag gagcatgctc gcctaggaga ttgtcgtttc     2040

ccgccttcag tttgcaagct gctctagccg tgtagccaat acgcaaaccg cctctccccg     2100

cgcgttggga attactagcg cgtgtcgaca cgcgtggcgc gccctggtat atttatatgt     2160

tgtcaaataa ctcaaaaacc ataaaagttt aagttagcaa gtgtgtacat ttttacttga     2220

acaaaaatat tcacctacta ctgttataaa tcattattaa acattagagt aaagaaatat     2280

ggatgataag aacaagagta gtgatatttt gacaacaatt ttgttgcaac atttgagaaa     2340

attttgttgt tctctctttt cattggtcaa aaacaataga gagagaaaaa ggaagaggga     2400

gaataaaaac ataatgtgag tatgagagag aaagttgtac aaaagttgta ccaaaatagt     2460

tgtacaaata tcattgagga atttgacaaa agctacacaa ataagggtta attgctgtaa     2520

ataaataagg atgacgcatt agagagatgt accattagag aatttttggc aagtcattaa     2580

aaagaaagaa taaattattt ttaaaattaa aagttgagtc atttgattaa acatgtgatt     2640

atttaatgaa ttgatgaaag agttggatta aagttgtatt agtaattaga atttggtgtc     2700

aaatttaatt tgacatttga tcttttccta tatattgccc catagagtca gttaactcat     2760

ttttatattt catagatcaa ataagagaaa taacggtata ttaatccctc caaaaaaaaa     2820

aaacggtata tttactaaaa aatctaagcc acgtaggagg ataacaggat ccccgtagga     2880

ggataacatc caatccaacc aatcacaaca atcctgatga gataacccac tttaagccca     2940

cgcatctgtg gcacatctac attatctaaa tcacacattc ttccacacat ctgagccaca     3000

caaaaaccaa tccacatctt tatcacccat tctataaaaa atcacacttt gtgagtctac     3060

actttgattc ccttcaaaca catacaaaga gaagagacta attaattaat taatcatctt     3120

gagagaaaat gagtcttcta accgaggtcg aaacgcctat cagaaacgaa tgggggtgca     3180

gatgcaacga ttcaagtgat cctcttgttg ttgccgcaag tataattggg attgtgcacc     3240

tgatattgtg gattattgat cgcctttttt ccaaaagcat ttatcgtatc tttaaacacg     3300

gtttaaaaag agggccttct acggaaggag taccagagtc tatgagggaa gaatatcgag     3360

aggaacagca gaatgctgtg gatgctgacg atggtcattt tgtcagcata gagctggagt     3420

aagagctcta agttaaaatg cttcttcgtc tcctatttat aatatggttt gttattgtta     3480

attttgttct tgtagaagag cttaattaat cgttgttgtt atgaaatact atttgtatga     3540

gatgaactgg tgtaatgtaa ttcatttaca taagtggagt cagaatcaga atgtttcctc     3600

cataactaac tagacatgaa gacctgccgc gtacaattgt cttatatttg aacaactaaa     3660

attgaacatc ttttgccaca actttataag tggttaatat agctcaaata tatggtcaag     3720

ttcaatagat taataatgga aatatcagtt atcgaaattc attaacaatc aacttaacgt     3780

tattaactac taattttata tcatcccctt tgataaatga tagtacacca attaggaagg     3840

aactaggaga ttgtcgtttc ccgccttcag tttgcaagct gctctagccg tgtagccaat     3900

acgcaaaccg cctctccccg cgcgttggga attactagcg cgtgtcgaca agcttgcatg     3960

ccggtcaaca tggtggagca cgacacactt gtctactcca aaaatatcaa agatacagtc     4020

tcagaagacc aaagggcaat tgagactttt caacaaaggg taatatccgg aaacctcctc     4080

ggattccatt gcccagctat ctgtcacttt attgtgaaga tagtggaaaa ggaaggtggc     4140

tcctacaaat gccatcattg cgataaagga aaggccatcg ttgaagatgc ctctgccgac     4200

agtggtccca aagatggacc cccacccacg aggagcatcg tggaaaaaga agacgttcca     4260

accacgtctt caaagcaagt ggattgatgt gataacatgg tggagcacga cacacttgtc     4320

tactccaaaa atatcaaaga tacagtctca gaagaccaaa gggcaattga gacttttcaa     4380

caaagggtaa tatccggaaa cctcctcgga ttccattgcc cagctatctg tcactttatt     4440

gtgaagatag tggaaaagga aggtggctcc tacaaatgcc atcattgcga taaaggaaag     4500

gccatcgttg aagatgcctc tgccgacagt ggtcccaaag atggaccccc acccacgagg     4560

agcatcgtgg aaaaagaaga cgttccaacc acgtcttcaa agcaagtgga ttgatgtgat     4620

atctccactg acgtaaggga tgacgcacaa tcccactatc cttcgcaaga cccttcctct     4680

atataaggaa gttcatttca tttggagagg tattaaaatc ttaataggtt ttgataaaag     4740

cgaacgtggg gaaacccgaa ccaaaccttc ttctaaactc tctctcatct ctcttaaagc     4800

aaacttctct cttgtctttc ttgcgtgagc gatcttcaac gttgtcagat cgtgcttcgg     4860

caccagtaca atggcgaaaa acgttgcgat tttcggctta ttgttttctc ttcttgtgtt     4920

ggttccttct cagatcttcg cgacgtcact cctcagccaa aacgacaccc ccatctgtct     4980

atccactggc ccctggatct gctgcccaaa ctaactccat ggtgaccctg ggatgcctgg     5040

tcaagggcta tttccctgag ccagtgacag tgacctggaa ctctggatcc ctgtccagcg     5100

gtgtgcacac cttcccagct gtcctgcagt ctgacctcta cactctgagc agctcagtga     5160

ctgtcccctc cagcacctgg cccagcgaga ccgtcacctg caacgttgcc cacccggcca     5220

gcagcaccaa ggtggacaag aaaattgtgc ccagggattg tggttgtaag ccttgcatat     5280

gtacagtccc agaagtatca tctgtcttca tcttcccccc aaagcccaag gatgtgctca     5340

ccattactct gactcctaag gtcacgtgtg ttgtggtaga catcagcaag gatgatcccg     5400

aggtccagtt cagctggttt gtagatgatg tggaggtgca cacagctcag acgcaacccc     5460

gggaggagca gttcaacagc actttccgct cagtcagtga acttcccatc atgcaccagg     5520

actggctcaa tggcaaggag acgtccagat tttggcgatc tattcaactg tcgccagttc     5580

attggtactg gtagtctccc tgggggcaat cagtttctgg atgtgctcta atgggtctct     5640

acagtgtaga atatgtattt aaaggcctat tttctttagt ttgaatttac tgttattcgg     5700

tgtgcatttc tatgtttggt gagcggtttt ctgtgctcag agtgtgttta ttttatgtaa     5760

tttaatttct ttgtgagctc ctgtttagca ggtcgtccct tcagcaagga cacaaaaaga     5820

ttttaatttt attatcgttc aaacatttgg caataaagtt tcttaagatt gaatcctgtt     5880

gccggtcttg cgatgattat catataattt ctgttgaatt acgttaagca tgtaataatt     5940

aacatgtaat gcatgacgtt atttatgaga tgggttttta tgattagagt cccgcaatta     6000

tacatttaat acgcgataga aaacaaaata tagcgcgcaa actaggataa attatcgcgc     6060

gcggtgtcat ctatgttact agatctctag agtctcaagc ttggcgcgcc cacgtgacta     6120

gtggcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt     6180

aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc     6240

gatcgccctt cccaacagtt gcgcagcctg aatggcgaat gctagagcag cttgagcttg     6300

gatcagattg tcgtttcccg ccttcagttt aaactatcag tgtttgacag gatatattgg     6360

cgggtaaacc taagagaaaa gagcgttta                                       6389


<210>  57
<211>  3060
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Construct 8352 from 2X35S prom to NOS term

<400>  57
gtcaacatgg tggagcacga cacacttgtc tactccaaaa atatcaaaga tacagtctca       60

gaagaccaaa gggcaattga gacttttcaa caaagggtaa tatccggaaa cctcctcgga      120

ttccattgcc cagctatctg tcactttatt gtgaagatag tggaaaagga aggtggctcc      180

tacaaatgcc atcattgcga taaaggaaag gccatcgttg aagatgcctc tgccgacagt      240

ggtcccaaag atggaccccc acccacgagg agcatcgtgg aaaaagaaga cgttccaacc      300

acgtcttcaa agcaagtgga ttgatgtgat aacatggtgg agcacgacac acttgtctac      360

tccaaaaata tcaaagatac agtctcagaa gaccaaaggg caattgagac ttttcaacaa      420

agggtaatat ccggaaacct cctcggattc cattgcccag ctatctgtca ctttattgtg      480

aagatagtgg aaaaggaagg tggctcctac aaatgccatc attgcgataa aggaaaggcc      540

atcgttgaag atgcctctgc cgacagtggt cccaaagatg gacccccacc cacgaggagc      600

atcgtggaaa aagaagacgt tccaaccacg tcttcaaagc aagtggattg atgtgatatc      660

tccactgacg taagggatga cgcacaatcc cactatcctt cgcaagaccc ttcctctata      720

taaggaagtt catttcattt ggagaggtat taaaatctta ataggttttg ataaaagcga      780

acgtggggaa acccgaacca aaccttcttc taaactctct ctcatctctc ttaaagcaaa      840

cttctctctt gtctttcttg cgtgagcgat cttcaacgtt gtcagatcgt gcttcggcac      900

cagtacaatg gcgaaaaacg ttgcgatttt cggcttattg ttttctcttc ttgtgttggt      960

tccttctcag atcttcgcgg atcgaatctg cactgggata acatcttcaa actcacctca     1020

tgtggtcaaa acagctactc aaggggaggt caatgtgact ggcgtgatac cactgacaac     1080

aacaccaaca aaatcttatt ttgcaaatct caaaggaaca aggaccagag ggaaactatg     1140

cccggactgt ctcaactgta cagatctgga tgtggccttg ggcaggccaa tgtgtgtggg     1200

gaccacacct tctgctaaag cttcaatact ccatgaggtc agacctgtta catccgggtg     1260

ctttcctata atgcacgaca gaacaaaaat caggcaacta cccaatcttc tcagaggata     1320

tgaaaagatc aggttatcaa cccaaaacgt tatcgatgca gaaaaagcac caggaggacc     1380

ctacagactt ggaaccgccg gatcttgccc taacgctacc agtaaaatcg gattttttgc     1440

aacaatggct tgggctgtcc caaaggacaa ctacaaaaat gcaacgaacc cactaacagt     1500

ggaagtacca tacatttgta cagaagggga agaccaaatt actgtttggg ggttccattc     1560

ggataacaaa acccaaatga agagcctcta tggagactca aatcctcaaa agttcacctc     1620

atctgctaat ggagtaacca cgcattatgt ttctcagatt ggcgacttcc cagatcaaac     1680

agaagacgga ggactaccac aaagcggcag aattgttgtt gattacatga tgcaaaaacc     1740

tgggaaaaca ggaacaattg tctatcaaag gggtgttttg ttgcctcaaa aggtgtggtg     1800

cgcgagtggc aggagcaaag taataaaagg gtcattgcct ttaattggtg aagcagattg     1860

ccttcatgaa gaatacggtg gattaaacaa aagcaagcct tactacacag gaaaacatgc     1920

aaaagccata ggaaattgcc caatatgggt aaaaacacct ttgaagcttg ccaatggaac     1980

caaatataga cctcctggtg gaggatggga aggaatgatt gcaggttggc acggatacac     2040

atctcacgga gcacatggag tggcagtggc ggcagacctt aagagtacac aagaagctat     2100

aaataagata acaaaaaatc tcaattcttt gagtgaacta gaagtaaaga accttcaaag     2160

actaagtggt gccatggatg aactccacaa cgaaatactc gagctggatg aaaaagtgga     2220

tgatctcaga gctgacacta taagctcaca aatagaactt gcagtcttgc tttccaacga     2280

aggaataata aacagtgaag acgagcatct attggcactt gagagaaaac taaagaaaat     2340

gctgggtccc tctgctgtag acataggaaa cggatgcttc gaaaccaaac acaaatgcaa     2400

ccagacctgc ttagacagga tagctgctgg cacctttaat gcaggagaat tttctctccc     2460

cacttttgat tcattgaaca ttactgctgc atctttaaat gatgatggat tggataacta     2520

ccagattttg gcgatctatt caactgtcgc cagttcattg gtactggtag tctccctggg     2580

ggcaatcagt ttctggatgt gctctaatgg gtctctacag tgtagaatat gtatttaaag     2640

gcctattttc tttagtttga atttactgtt attcggtgtg catttctatg tttggtgagc     2700

ggttttctgt gctcagagtg tgtttatttt atgtaattta atttctttgt gagctcctgt     2760

ttagcaggtc gtcccttcag caaggacaca aaaagatttt aattttatta tcgttcaaac     2820

atttggcaat aaagtttctt aagattgaat cctgttgccg gtcttgcgat gattatcata     2880

taatttctgt tgaattacgt taagcatgta ataattaaca tgtaatgcat gacgttattt     2940

atgagatggg tttttatgat tagagtcccg caattataca tttaatacgc gatagaaaac     3000

aaaatatagc gcgcaaacta ggataaatta tcgcgcgcgg tgtcatctat gttactagat     3060


<210>  58
<211>  3054
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Construct 7281 from 2X35S prom to NOS term

<400>  58
gtcaacatgg tggagcacga cacacttgtc tactccaaaa atatcaaaga tacagtctca       60

gaagaccaaa gggcaattga gacttttcaa caaagggtaa tatccggaaa cctcctcgga      120

ttccattgcc cagctatctg tcactttatt gtgaagatag tggaaaagga aggtggctcc      180

tacaaatgcc atcattgcga taaaggaaag gccatcgttg aagatgcctc tgccgacagt      240

ggtcccaaag atggaccccc acccacgagg agcatcgtgg aaaaagaaga cgttccaacc      300

acgtcttcaa agcaagtgga ttgatgtgat aacatggtgg agcacgacac acttgtctac      360

tccaaaaata tcaaagatac agtctcagaa gaccaaaggg caattgagac ttttcaacaa      420

agggtaatat ccggaaacct cctcggattc cattgcccag ctatctgtca ctttattgtg      480

aagatagtgg aaaaggaagg tggctcctac aaatgccatc attgcgataa aggaaaggcc      540

atcgttgaag atgcctctgc cgacagtggt cccaaagatg gacccccacc cacgaggagc      600

atcgtggaaa aagaagacgt tccaaccacg tcttcaaagc aagtggattg atgtgatatc      660

tccactgacg taagggatga cgcacaatcc cactatcctt cgcaagaccc ttcctctata      720

taaggaagtt catttcattt ggagaggtat taaaatctta ataggttttg ataaaagcga      780

acgtggggaa acccgaacca aaccttcttc taaactctct ctcatctctc ttaaagcaaa      840

cttctctctt gtctttcttg cgtgagcgat cttcaacgtt gtcagatcgt gcttcggcac      900

cagtacaatg gcgaaaaacg ttgcgatttt cggcttattg ttttctcttc ttgtgttggt      960

tccttctcag atcttcgcgc aaaaaatccc tggaaatgac aatagcacgg caacgctgtg     1020

ccttgggcac catgcagtac caaacggaac gatagtgaaa acaatcacga atgaccgaat     1080

tgaagttact aatgctactg agctggttca gaattcctca ataggtgaaa tatgcgacag     1140

tcctcatcag atccttgatg gagaaaactg cacactaata gatgctctat tgggagaccc     1200

tcagtgtgat ggctttcaaa ataagaaatg ggaccttttt gttgaacgaa acaaagccta     1260

cagcaactgt tacccttatg atgtgccgga ttatgcctcc cttaggtcac tagttgcctc     1320

atccggcaca ctggagttta acaatgaaag cttcaattgg gctggagtca ctcaaaacgg     1380

aacaagttct tcttgcataa ggggatctaa gagtagtttc tttagtagat taaattggtt     1440

gacccactta aactccaaat acccagcatt aaacgtgact atgccaaaca atgaacaatt     1500

tgacaaattg tacatttggg gggttcacca cccgggtacg gacaaggacc aaatcagcct     1560

gtatgcacaa tcatcaggaa gaatcacagt atctaccaaa agaagccaac aagctgtaat     1620

cccgaatatc ggatctagac ccagaataag ggatatccct agcagaataa gcatctattg     1680

gacaatagta aaaccgggag acatactttt gattaacagc acagggaatc taattgctcc     1740

taggggttac ttcaaaatac gaagtgggaa aagctcaata atgagatcag atgcacccat     1800

tggcaaatgc aagtctgaat gcatcactcc aaatggaagc attcccaatg acaaaccatt     1860

ccaaaatgta aacaggatca catacggggc ctgtcccaga tatgttaagc aaagcactct     1920

gaaattggca acaggaatgc gaaatgtacc agagagacaa actagaggca tatttggcgc     1980

aatagcgggt ttcatagaaa atggttggga gggaatggtg gatggttggt acggcttcag     2040

gcatcaaaat tctgagggaa gaggacaagc agcagatctc aaaagcactc aagcagcaat     2100

cgatcaaatc aatgggaagc tggctcgagt tatcgggaaa accaacgaga aattccatca     2160

gattgaaaaa gaattctcag aagtagaagg gagaattcag gaccttgaga aatatgttga     2220

ggacacaaaa atagatctct ggtcatacaa cgcggagctt cttgttgccc tggagaacca     2280

acatacaatt gatctaactg actcagaaat gaacaaactg tttgaaaaaa caaagaagca     2340

actgagggaa aatgctgagg atatgggcaa tggttgtttc aaaatatacc acaaatgtga     2400

caatgcctgc atgggatcaa tcagaaatgg aacttatgac cacaacgtat acagggatga     2460

agcattaaac aaccggttcc agatcaaggg agttgagctg aagtcagggt acaaagattg     2520

gatcctatgg atttcctttg ccatatcatc ccttgtactg ttagttgctt tgttggggtt     2580

catcatgtgg gcctgccaaa agggcaacat taggtgcaac atttgcattt gaaggcctat     2640

tttctttagt ttgaatttac tgttattcgg tgtgcatttc tatgtttggt gagcggtttt     2700

ctgtgctcag agtgtgttta ttttatgtaa tttaatttct ttgtgagctc ctgtttagca     2760

ggtcgtccct tcagcaagga cacaaaaaga ttttaatttt attatcgttc aaacatttgg     2820

caataaagtt tcttaagatt gaatcctgtt gccggtcttg cgatgattat catataattt     2880

ctgttgaatt acgttaagca tgtaataatt aacatgtaat gcatgacgtt atttatgaga     2940

tgggttttta tgattagagt cccgcaatta tacatttaat acgcgataga aaacaaaata     3000

tagcgcgcaa actaggataa attatcgcgc gcggtgtcat ctatgttact agat           3054


<210>  59
<211>  3054
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Construct 8179 from 2X35S prom to NOS term

<400>  59
gtcaacatgg tggagcacga cacacttgtc tactccaaaa atatcaaaga tacagtctca       60

gaagaccaaa gggcaattga gacttttcaa caaagggtaa tatccggaaa cctcctcgga      120

ttccattgcc cagctatctg tcactttatt gtgaagatag tggaaaagga aggtggctcc      180

tacaaatgcc atcattgcga taaaggaaag gccatcgttg aagatgcctc tgccgacagt      240

ggtcccaaag atggaccccc acccacgagg agcatcgtgg aaaaagaaga cgttccaacc      300

acgtcttcaa agcaagtgga ttgatgtgat aacatggtgg agcacgacac acttgtctac      360

tccaaaaata tcaaagatac agtctcagaa gaccaaaggg caattgagac ttttcaacaa      420

agggtaatat ccggaaacct cctcggattc cattgcccag ctatctgtca ctttattgtg      480

aagatagtgg aaaaggaagg tggctcctac aaatgccatc attgcgataa aggaaaggcc      540

atcgttgaag atgcctctgc cgacagtggt cccaaagatg gacccccacc cacgaggagc      600

atcgtggaaa aagaagacgt tccaaccacg tcttcaaagc aagtggattg atgtgatatc      660

tccactgacg taagggatga cgcacaatcc cactatcctt cgcaagaccc ttcctctata      720

taaggaagtt catttcattt ggagaggtat taaaatctta ataggttttg ataaaagcga      780

acgtggggaa acccgaacca aaccttcttc taaactctct ctcatctctc ttaaagcaaa      840

cttctctctt gtctttcttg cgtgagcgat cttcaacgtt gtcagatcgt gcttcggcac      900

cagtacaatg gcgaaaaacg ttgcgatttt cggcttattg ttttctcttc ttgtgttggt      960

tccttctcag atcttcgcgc aaaaaatccc tggaaatgac aatagcacgg caacgctgtg     1020

ccttgggcac catgcagtac caaacggaac gatagtgaaa acaatcacga atgaccgaat     1080

tgaagttact aatgctactg agctggttca gaattcctca ataggtgaaa tatgcgacag     1140

tcctcatcag atccttgatg gagaaaactg cacactaata gatgctctat tgggagaccc     1200

tcagtgtgat ggctttcaaa ataagaaatg ggaccttttt gttgaacgaa acaaagccta     1260

cagcaactgt ttcccttatg atgtgccgga ttatgcctcc cttaggtcac tagttgcctc     1320

atccggcaca ctggagttta acaatgaaag cttcaattgg gctggagtca ctcaaaacgg     1380

aacaagttct tcttgcataa ggggatctaa gagtagtttc tttagtagat taaattggtt     1440

gacccactta aactccaaat acccagcatt aaacgtgact atgccaaaca atgaacaatt     1500

tgacaaattg tacatttggg gggttcacca cccgggtacg gacaaggacc aaatcagcct     1560

gtatgcacaa tcatcaggaa gaatcacagt atctaccaaa agaagccaac aagctgtaat     1620

cccgaatatc ggatctagac ccagaataag ggatatccct agcagaataa gcatctattg     1680

gacaatagta aaaccgggag acatactttt gattaacagc acagggaatc taattgctcc     1740

taggggttac ttcaaaatac gaagtgggaa aagctcaata atgagatcag atgcacccat     1800

tggcaaatgc aagtctgaat gcatcactcc aaatggaagc attcccaatg acaaaccatt     1860

ccaaaatgta aacaggatca catacggggc ctgtcccaga tatgttaagc aaagcactct     1920

gaaattggca acaggaatgc gaaatgtacc agagagacaa actagaggca tatttggcgc     1980

aatagcgggt ttcatagaaa atggttggga gggaatggtg gatggttggt acggcttcag     2040

gcatcaaaat tctgagggaa gaggacaagc agcagatctc aaaagcactc aagcagcaat     2100

cgatcaaatc aatgggaagc tggctcgagt tatcgggaaa accaacgaga aattccatca     2160

gattgaaaaa gaattctcag aagtagaagg gagaattcag gaccttgaga aatatgttga     2220

ggacacaaaa atagatctct ggtcatacaa cgcggagctt cttgttgccc tggagaacca     2280

acatacaatt gatctaactg actcagaaat gaacaaactg tttgaaaaaa caaagaagca     2340

actgagggaa aatgctgagg atatgggcaa tggttgtttc aaaatatacc acaaatgtga     2400

caatgcctgc atgggatcaa tcagaaatgg aacttatgac cacaacgtat acagggatga     2460

agcattaaac aaccggttcc agatcaaggg agttgagctg aagtcagggt acaaagattg     2520

gatcctatgg atttcctttg ccatatcatc ccttgtactg ttagttgctt tgttggggtt     2580

catcatgtgg gcctgccaaa agggcaacat taggtgcaac atttgcattt gaaggcctat     2640

tttctttagt ttgaatttac tgttattcgg tgtgcatttc tatgtttggt gagcggtttt     2700

ctgtgctcag agtgtgttta ttttatgtaa tttaatttct ttgtgagctc ctgtttagca     2760

ggtcgtccct tcagcaagga cacaaaaaga ttttaatttt attatcgttc aaacatttgg     2820

caataaagtt tcttaagatt gaatcctgtt gccggtcttg cgatgattat catataattt     2880

ctgttgaatt acgttaagca tgtaataatt aacatgtaat gcatgacgtt atttatgaga     2940

tgggttttta tgattagagt cccgcaatta tacatttaat acgcgataga aaacaaaata     3000

tagcgcgcaa actaggataa attatcgcgc gcggtgtcat ctatgttact agat           3054


<210>  60
<211>  1725
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PDI-H3 A/Kansas/14/2017

<400>  60
atggcgaaaa acgttgcgat tttcggctta ttgttttctc ttcttgtgtt ggttccttct       60

cagatcttcg cgcaaaaaat ccctggaaat gacaatagca cggcaacgct gtgccttggg      120

caccatgcag taccaaacgg aacgatagtg aaaacaatca cgaatgaccg aattgaagtt      180

actaatgcta ctgagctggt tcagaattcc tcaataggtg aaatatgcga cagtcctcat      240

cagatccttg atggagaaaa ctgcacacta atagatgctc tattgggaga ccctcagtgt      300

gatggctttc aaaataagaa atgggacctt tttgttgaac gaaacaaagc ctacagcaac      360

tgttaccctt atgatgtgcc ggattatgcc tcccttaggt cactagttgc ctcatccggc      420

acactggagt ttaacaatga aagcttcaat tgggctggag tcactcaaaa cggaacaagt      480

tcttcttgca taaggggatc taagagtagt ttctttagta gattaaattg gttgacccac      540

ttaaactcca aatacccagc attaaacgtg actatgccaa acaatgaaca atttgacaaa      600

ttgtacattt ggggggttca ccacccgggt acggacaagg accaaatcag cctgtatgca      660

caatcatcag gaagaatcac agtatctacc aaaagaagcc aacaagctgt aatcccgaat      720

atcggatcta gacccagaat aagggatatc cctagcagaa taagcatcta ttggacaata      780

gtaaaaccgg gagacatact tttgattaac agcacaggga atctaattgc tcctaggggt      840

tacttcaaaa tacgaagtgg gaaaagctca ataatgagat cagatgcacc cattggcaaa      900

tgcaagtctg aatgcatcac tccaaatgga agcattccca atgacaaacc attccaaaat      960

gtaaacagga tcacatacgg ggcctgtccc agatatgtta agcaaagcac tctgaaattg     1020

gcaacaggaa tgcgaaatgt accagagaga caaactagag gcatatttgg cgcaatagcg     1080

ggtttcatag aaaatggttg ggagggaatg gtggatggtt ggtacggctt caggcatcaa     1140

aattctgagg gaagaggaca agcagcagat ctcaaaagca ctcaagcagc aatcgatcaa     1200

atcaatggga agctggctcg agttatcggg aaaaccaacg agaaattcca tcagattgaa     1260

aaagaattct cagaagtaga agggagaatt caggaccttg agaaatatgt tgaggacaca     1320

aaaatagatc tctggtcata caacgcggag cttcttgttg ccctggagaa ccaacataca     1380

attgatctaa ctgactcaga aatgaacaaa ctgtttgaaa aaacaaagaa gcaactgagg     1440

gaaaatgctg aggatatggg caatggttgt ttcaaaatat accacaaatg tgacaatgcc     1500

tgcatgggat caatcagaaa tggaacttat gaccacaacg tatacaggga tgaagcatta     1560

aacaaccggt tccagatcaa gggagttgag ctgaagtcag ggtacaaaga ttggatccta     1620

tggatttcct ttgccatatc atcccttgta ctgttagttg ctttgttggg gttcatcatg     1680

tgggcctgcc aaaagggcaa cattaggtgc aacatttgca tttga                     1725


<210>  61
<211>  574
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  PDI-H3 A/Kansas/14/2017

<400>  61

Met Ala Lys Asn Val Ala Ile Phe Gly Leu Leu Phe Ser Leu Leu Val 
1               5                   10                  15      


Leu Val Pro Ser Gln Ile Phe Ala Gln Lys Ile Pro Gly Asn Asp Asn 
            20                  25                  30          


Ser Thr Ala Thr Leu Cys Leu Gly His His Ala Val Pro Asn Gly Thr 
        35                  40                  45              


Ile Val Lys Thr Ile Thr Asn Asp Arg Ile Glu Val Thr Asn Ala Thr 
    50                  55                  60                  


Glu Leu Val Gln Asn Ser Ser Ile Gly Glu Ile Cys Asp Ser Pro His 
65                  70                  75                  80  


Gln Ile Leu Asp Gly Glu Asn Cys Thr Leu Ile Asp Ala Leu Leu Gly 
                85                  90                  95      


Asp Pro Gln Cys Asp Gly Phe Gln Asn Lys Lys Trp Asp Leu Phe Val 
            100                 105                 110         


Glu Arg Asn Lys Ala Tyr Ser Asn Cys Tyr Pro Tyr Asp Val Pro Asp 
        115                 120                 125             


Tyr Ala Ser Leu Arg Ser Leu Val Ala Ser Ser Gly Thr Leu Glu Phe 
    130                 135                 140                 


Asn Asn Glu Ser Phe Asn Trp Ala Gly Val Thr Gln Asn Gly Thr Ser 
145                 150                 155                 160 


Ser Ser Cys Ile Arg Gly Ser Lys Ser Ser Phe Phe Ser Arg Leu Asn 
                165                 170                 175     


Trp Leu Thr His Leu Asn Ser Lys Tyr Pro Ala Leu Asn Val Thr Met 
            180                 185                 190         


Pro Asn Asn Glu Gln Phe Asp Lys Leu Tyr Ile Trp Gly Val His His 
        195                 200                 205             


Pro Gly Thr Asp Lys Asp Gln Ile Ser Leu Tyr Ala Gln Ser Ser Gly 
    210                 215                 220                 


Arg Ile Thr Val Ser Thr Lys Arg Ser Gln Gln Ala Val Ile Pro Asn 
225                 230                 235                 240 


Ile Gly Ser Arg Pro Arg Ile Arg Asp Ile Pro Ser Arg Ile Ser Ile 
                245                 250                 255     


Tyr Trp Thr Ile Val Lys Pro Gly Asp Ile Leu Leu Ile Asn Ser Thr 
            260                 265                 270         


Gly Asn Leu Ile Ala Pro Arg Gly Tyr Phe Lys Ile Arg Ser Gly Lys 
        275                 280                 285             


Ser Ser Ile Met Arg Ser Asp Ala Pro Ile Gly Lys Cys Lys Ser Glu 
    290                 295                 300                 


Cys Ile Thr Pro Asn Gly Ser Ile Pro Asn Asp Lys Pro Phe Gln Asn 
305                 310                 315                 320 


Val Asn Arg Ile Thr Tyr Gly Ala Cys Pro Arg Tyr Val Lys Gln Ser 
                325                 330                 335     


Thr Leu Lys Leu Ala Thr Gly Met Arg Asn Val Pro Glu Arg Gln Thr 
            340                 345                 350         


Arg Gly Ile Phe Gly Ala Ile Ala Gly Phe Ile Glu Asn Gly Trp Glu 
        355                 360                 365             


Gly Met Val Asp Gly Trp Tyr Gly Phe Arg His Gln Asn Ser Glu Gly 
    370                 375                 380                 


Arg Gly Gln Ala Ala Asp Leu Lys Ser Thr Gln Ala Ala Ile Asp Gln 
385                 390                 395                 400 


Ile Asn Gly Lys Leu Ala Arg Val Ile Gly Lys Thr Asn Glu Lys Phe 
                405                 410                 415     


His Gln Ile Glu Lys Glu Phe Ser Glu Val Glu Gly Arg Ile Gln Asp 
            420                 425                 430         


Leu Glu Lys Tyr Val Glu Asp Thr Lys Ile Asp Leu Trp Ser Tyr Asn 
        435                 440                 445             


Ala Glu Leu Leu Val Ala Leu Glu Asn Gln His Thr Ile Asp Leu Thr 
    450                 455                 460                 


Asp Ser Glu Met Asn Lys Leu Phe Glu Lys Thr Lys Lys Gln Leu Arg 
465                 470                 475                 480 


Glu Asn Ala Glu Asp Met Gly Asn Gly Cys Phe Lys Ile Tyr His Lys 
                485                 490                 495     


Cys Asp Asn Ala Cys Met Gly Ser Ile Arg Asn Gly Thr Tyr Asp His 
            500                 505                 510         


Asn Val Tyr Arg Asp Glu Ala Leu Asn Asn Arg Phe Gln Ile Lys Gly 
        515                 520                 525             


Val Glu Leu Lys Ser Gly Tyr Lys Asp Trp Ile Leu Trp Ile Ser Phe 
    530                 535                 540                 


Ala Ile Ser Ser Leu Val Leu Leu Val Ala Leu Leu Gly Phe Ile Met 
545                 550                 555                 560 


Trp Ala Cys Gln Lys Gly Asn Ile Arg Cys Asn Ile Cys Ile 
                565                 570                 


<210>  62
<211>  57
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer IF-H3NewJer.c

<400>  62
tctcagatct tcgcgcaaaa aatccctgga aatgacaata gcacggcaac gctgtgc          57


<210>  63
<211>  51
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer IF-H3_Swi_13.r

<400>  63
actaaagaaa ataggccttc aaatgcaaat gttgcaccta atgttgccct t                51


<210>  64
<211>  1725
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PDI-H3 A/Kansas/14/2017 Y98F

<400>  64
atggcgaaaa acgttgcgat tttcggctta ttgttttctc ttcttgtgtt ggttccttct       60

cagatcttcg cgcaaaaaat ccctggaaat gacaatagca cggcaacgct gtgccttggg      120

caccatgcag taccaaacgg aacgatagtg aaaacaatca cgaatgaccg aattgaagtt      180

actaatgcta ctgagctggt tcagaattcc tcaataggtg aaatatgcga cagtcctcat      240

cagatccttg atggagaaaa ctgcacacta atagatgctc tattgggaga ccctcagtgt      300

gatggctttc aaaataagaa atgggacctt tttgttgaac gaaacaaagc ctacagcaac      360

tgtttccctt atgatgtgcc ggattatgcc tcccttaggt cactagttgc ctcatccggc      420

acactggagt ttaacaatga aagcttcaat tgggctggag tcactcaaaa cggaacaagt      480

tcttcttgca taaggggatc taagagtagt ttctttagta gattaaattg gttgacccac      540

ttaaactcca aatacccagc attaaacgtg actatgccaa acaatgaaca atttgacaaa      600

ttgtacattt ggggggttca ccacccgggt acggacaagg accaaatcag cctgtatgca      660

caatcatcag gaagaatcac agtatctacc aaaagaagcc aacaagctgt aatcccgaat      720

atcggatcta gacccagaat aagggatatc cctagcagaa taagcatcta ttggacaata      780

gtaaaaccgg gagacatact tttgattaac agcacaggga atctaattgc tcctaggggt      840

tacttcaaaa tacgaagtgg gaaaagctca ataatgagat cagatgcacc cattggcaaa      900

tgcaagtctg aatgcatcac tccaaatgga agcattccca atgacaaacc attccaaaat      960

gtaaacagga tcacatacgg ggcctgtccc agatatgtta agcaaagcac tctgaaattg     1020

gcaacaggaa tgcgaaatgt accagagaga caaactagag gcatatttgg cgcaatagcg     1080

ggtttcatag aaaatggttg ggagggaatg gtggatggtt ggtacggctt caggcatcaa     1140

aattctgagg gaagaggaca agcagcagat ctcaaaagca ctcaagcagc aatcgatcaa     1200

atcaatggga agctggctcg agttatcggg aaaaccaacg agaaattcca tcagattgaa     1260

aaagaattct cagaagtaga agggagaatt caggaccttg agaaatatgt tgaggacaca     1320

aaaatagatc tctggtcata caacgcggag cttcttgttg ccctggagaa ccaacataca     1380

attgatctaa ctgactcaga aatgaacaaa ctgtttgaaa aaacaaagaa gcaactgagg     1440

gaaaatgctg aggatatggg caatggttgt ttcaaaatat accacaaatg tgacaatgcc     1500

tgcatgggat caatcagaaa tggaacttat gaccacaacg tatacaggga tgaagcatta     1560

aacaaccggt tccagatcaa gggagttgag ctgaagtcag ggtacaaaga ttggatccta     1620

tggatttcct ttgccatatc atcccttgta ctgttagttg ctttgttggg gttcatcatg     1680

tgggcctgcc aaaagggcaa cattaggtgc aacatttgca tttga                     1725


<210>  65
<211>  574
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  PDI-H3 A/Kansas/14/2017 Y98F

<400>  65

Met Ala Lys Asn Val Ala Ile Phe Gly Leu Leu Phe Ser Leu Leu Val 
1               5                   10                  15      


Leu Val Pro Ser Gln Ile Phe Ala Gln Lys Ile Pro Gly Asn Asp Asn 
            20                  25                  30          


Ser Thr Ala Thr Leu Cys Leu Gly His His Ala Val Pro Asn Gly Thr 
        35                  40                  45              


Ile Val Lys Thr Ile Thr Asn Asp Arg Ile Glu Val Thr Asn Ala Thr 
    50                  55                  60                  


Glu Leu Val Gln Asn Ser Ser Ile Gly Glu Ile Cys Asp Ser Pro His 
65                  70                  75                  80  


Gln Ile Leu Asp Gly Glu Asn Cys Thr Leu Ile Asp Ala Leu Leu Gly 
                85                  90                  95      


Asp Pro Gln Cys Asp Gly Phe Gln Asn Lys Lys Trp Asp Leu Phe Val 
            100                 105                 110         


Glu Arg Asn Lys Ala Tyr Ser Asn Cys Phe Pro Tyr Asp Val Pro Asp 
        115                 120                 125             


Tyr Ala Ser Leu Arg Ser Leu Val Ala Ser Ser Gly Thr Leu Glu Phe 
    130                 135                 140                 


Asn Asn Glu Ser Phe Asn Trp Ala Gly Val Thr Gln Asn Gly Thr Ser 
145                 150                 155                 160 


Ser Ser Cys Ile Arg Gly Ser Lys Ser Ser Phe Phe Ser Arg Leu Asn 
                165                 170                 175     


Trp Leu Thr His Leu Asn Ser Lys Tyr Pro Ala Leu Asn Val Thr Met 
            180                 185                 190         


Pro Asn Asn Glu Gln Phe Asp Lys Leu Tyr Ile Trp Gly Val His His 
        195                 200                 205             


Pro Gly Thr Asp Lys Asp Gln Ile Ser Leu Tyr Ala Gln Ser Ser Gly 
    210                 215                 220                 


Arg Ile Thr Val Ser Thr Lys Arg Ser Gln Gln Ala Val Ile Pro Asn 
225                 230                 235                 240 


Ile Gly Ser Arg Pro Arg Ile Arg Asp Ile Pro Ser Arg Ile Ser Ile 
                245                 250                 255     


Tyr Trp Thr Ile Val Lys Pro Gly Asp Ile Leu Leu Ile Asn Ser Thr 
            260                 265                 270         


Gly Asn Leu Ile Ala Pro Arg Gly Tyr Phe Lys Ile Arg Ser Gly Lys 
        275                 280                 285             


Ser Ser Ile Met Arg Ser Asp Ala Pro Ile Gly Lys Cys Lys Ser Glu 
    290                 295                 300                 


Cys Ile Thr Pro Asn Gly Ser Ile Pro Asn Asp Lys Pro Phe Gln Asn 
305                 310                 315                 320 


Val Asn Arg Ile Thr Tyr Gly Ala Cys Pro Arg Tyr Val Lys Gln Ser 
                325                 330                 335     


Thr Leu Lys Leu Ala Thr Gly Met Arg Asn Val Pro Glu Arg Gln Thr 
            340                 345                 350         


Arg Gly Ile Phe Gly Ala Ile Ala Gly Phe Ile Glu Asn Gly Trp Glu 
        355                 360                 365             


Gly Met Val Asp Gly Trp Tyr Gly Phe Arg His Gln Asn Ser Glu Gly 
    370                 375                 380                 


Arg Gly Gln Ala Ala Asp Leu Lys Ser Thr Gln Ala Ala Ile Asp Gln 
385                 390                 395                 400 


Ile Asn Gly Lys Leu Ala Arg Val Ile Gly Lys Thr Asn Glu Lys Phe 
                405                 410                 415     


His Gln Ile Glu Lys Glu Phe Ser Glu Val Glu Gly Arg Ile Gln Asp 
            420                 425                 430         


Leu Glu Lys Tyr Val Glu Asp Thr Lys Ile Asp Leu Trp Ser Tyr Asn 
        435                 440                 445             


Ala Glu Leu Leu Val Ala Leu Glu Asn Gln His Thr Ile Asp Leu Thr 
    450                 455                 460                 


Asp Ser Glu Met Asn Lys Leu Phe Glu Lys Thr Lys Lys Gln Leu Arg 
465                 470                 475                 480 


Glu Asn Ala Glu Asp Met Gly Asn Gly Cys Phe Lys Ile Tyr His Lys 
                485                 490                 495     


Cys Asp Asn Ala Cys Met Gly Ser Ile Arg Asn Gly Thr Tyr Asp His 
            500                 505                 510         


Asn Val Tyr Arg Asp Glu Ala Leu Asn Asn Arg Phe Gln Ile Lys Gly 
        515                 520                 525             


Val Glu Leu Lys Ser Gly Tyr Lys Asp Trp Ile Leu Trp Ile Ser Phe 
    530                 535                 540                 


Ala Ile Ser Ser Leu Val Leu Leu Val Ala Leu Leu Gly Phe Ile Met 
545                 550                 555                 560 


Trp Ala Cys Gln Lys Gly Asn Ile Arg Cys Asn Ile Cys Ile 
                565                 570                 


<210>  66
<211>  48
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer H3_Kansas(Y98F).c

<400>  66
cctacagcaa ctgtttccct tatgatgtgc cggattatgc ctccctta                    48


<210>  67
<211>  50
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer H3_Kansas(Y98F).r

<400>  67
ccggcacatc ataagggaaa cagttgctgt aggctttgtt tcgttcaaca                  50


<210>  68
<211>  1725
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PDI-H3 A/Kansas/14/2017 Y98F, S136D

<400>  68
atggcgaaaa acgttgcgat tttcggctta ttgttttctc ttcttgtgtt ggttccttct       60

cagatcttcg cgcaaaaaat ccctggaaat gacaatagca cggcaacgct gtgccttggg      120

caccatgcag taccaaacgg aacgatagtg aaaacaatca cgaatgaccg aattgaagtt      180

actaatgcta ctgagctggt tcagaattcc tcaataggtg aaatatgcga cagtcctcat      240

cagatccttg atggagaaaa ctgcacacta atagatgctc tattgggaga ccctcagtgt      300

gatggctttc aaaataagaa atgggacctt tttgttgaac gaaacaaagc ctacagcaac      360

tgtttccctt atgatgtgcc ggattatgcc tcccttaggt cactagttgc ctcatccggc      420

acactggagt ttaacaatga aagcttcaat tgggctggag tcactcaaaa cggaacagac      480

tcttcttgca taaggggatc taagagtagt ttctttagta gattaaattg gttgacccac      540

ttaaactcca aatacccagc attaaacgtg actatgccaa acaatgaaca atttgacaaa      600

ttgtacattt ggggggttca ccacccgggt acggacaagg accaaatcag cctgtatgca      660

caatcatcag gaagaatcac agtatctacc aaaagaagcc aacaagctgt aatcccgaat      720

atcggatcta gacccagaat aagggatatc cctagcagaa taagcatcta ttggacaata      780

gtaaaaccgg gagacatact tttgattaac agcacaggga atctaattgc tcctaggggt      840

tacttcaaaa tacgaagtgg gaaaagctca ataatgagat cagatgcacc cattggcaaa      900

tgcaagtctg aatgcatcac tccaaatgga agcattccca atgacaaacc attccaaaat      960

gtaaacagga tcacatacgg ggcctgtccc agatatgtta agcaaagcac tctgaaattg     1020

gcaacaggaa tgcgaaatgt accagagaga caaactagag gcatatttgg cgcaatagcg     1080

ggtttcatag aaaatggttg ggagggaatg gtggatggtt ggtacggctt caggcatcaa     1140

aattctgagg gaagaggaca agcagcagat ctcaaaagca ctcaagcagc aatcgatcaa     1200

atcaatggga agctggctcg agttatcggg aaaaccaacg agaaattcca tcagattgaa     1260

aaagaattct cagaagtaga agggagaatt caggaccttg agaaatatgt tgaggacaca     1320

aaaatagatc tctggtcata caacgcggag cttcttgttg ccctggagaa ccaacataca     1380

attgatctaa ctgactcaga aatgaacaaa ctgtttgaaa aaacaaagaa gcaactgagg     1440

gaaaatgctg aggatatggg caatggttgt ttcaaaatat accacaaatg tgacaatgcc     1500

tgcatgggat caatcagaaa tggaacttat gaccacaacg tatacaggga tgaagcatta     1560

aacaaccggt tccagatcaa gggagttgag ctgaagtcag ggtacaaaga ttggatccta     1620

tggatttcct ttgccatatc atcccttgta ctgttagttg ctttgttggg gttcatcatg     1680

tgggcctgcc aaaagggcaa cattaggtgc aacatttgca tttga                     1725


<210>  69
<211>  574
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  PDI-H3 A/Kansas/14/2017 Y98F, S136D

<400>  69

Met Ala Lys Asn Val Ala Ile Phe Gly Leu Leu Phe Ser Leu Leu Val 
1               5                   10                  15      


Leu Val Pro Ser Gln Ile Phe Ala Gln Lys Ile Pro Gly Asn Asp Asn 
            20                  25                  30          


Ser Thr Ala Thr Leu Cys Leu Gly His His Ala Val Pro Asn Gly Thr 
        35                  40                  45              


Ile Val Lys Thr Ile Thr Asn Asp Arg Ile Glu Val Thr Asn Ala Thr 
    50                  55                  60                  


Glu Leu Val Gln Asn Ser Ser Ile Gly Glu Ile Cys Asp Ser Pro His 
65                  70                  75                  80  


Gln Ile Leu Asp Gly Glu Asn Cys Thr Leu Ile Asp Ala Leu Leu Gly 
                85                  90                  95      


Asp Pro Gln Cys Asp Gly Phe Gln Asn Lys Lys Trp Asp Leu Phe Val 
            100                 105                 110         


Glu Arg Asn Lys Ala Tyr Ser Asn Cys Phe Pro Tyr Asp Val Pro Asp 
        115                 120                 125             


Tyr Ala Ser Leu Arg Ser Leu Val Ala Ser Ser Gly Thr Leu Glu Phe 
    130                 135                 140                 


Asn Asn Glu Ser Phe Asn Trp Ala Gly Val Thr Gln Asn Gly Thr Asp 
145                 150                 155                 160 


Ser Ser Cys Ile Arg Gly Ser Lys Ser Ser Phe Phe Ser Arg Leu Asn 
                165                 170                 175     


Trp Leu Thr His Leu Asn Ser Lys Tyr Pro Ala Leu Asn Val Thr Met 
            180                 185                 190         


Pro Asn Asn Glu Gln Phe Asp Lys Leu Tyr Ile Trp Gly Val His His 
        195                 200                 205             


Pro Gly Thr Asp Lys Asp Gln Ile Ser Leu Tyr Ala Gln Ser Ser Gly 
    210                 215                 220                 


Arg Ile Thr Val Ser Thr Lys Arg Ser Gln Gln Ala Val Ile Pro Asn 
225                 230                 235                 240 


Ile Gly Ser Arg Pro Arg Ile Arg Asp Ile Pro Ser Arg Ile Ser Ile 
                245                 250                 255     


Tyr Trp Thr Ile Val Lys Pro Gly Asp Ile Leu Leu Ile Asn Ser Thr 
            260                 265                 270         


Gly Asn Leu Ile Ala Pro Arg Gly Tyr Phe Lys Ile Arg Ser Gly Lys 
        275                 280                 285             


Ser Ser Ile Met Arg Ser Asp Ala Pro Ile Gly Lys Cys Lys Ser Glu 
    290                 295                 300                 


Cys Ile Thr Pro Asn Gly Ser Ile Pro Asn Asp Lys Pro Phe Gln Asn 
305                 310                 315                 320 


Val Asn Arg Ile Thr Tyr Gly Ala Cys Pro Arg Tyr Val Lys Gln Ser 
                325                 330                 335     


Thr Leu Lys Leu Ala Thr Gly Met Arg Asn Val Pro Glu Arg Gln Thr 
            340                 345                 350         


Arg Gly Ile Phe Gly Ala Ile Ala Gly Phe Ile Glu Asn Gly Trp Glu 
        355                 360                 365             


Gly Met Val Asp Gly Trp Tyr Gly Phe Arg His Gln Asn Ser Glu Gly 
    370                 375                 380                 


Arg Gly Gln Ala Ala Asp Leu Lys Ser Thr Gln Ala Ala Ile Asp Gln 
385                 390                 395                 400 


Ile Asn Gly Lys Leu Ala Arg Val Ile Gly Lys Thr Asn Glu Lys Phe 
                405                 410                 415     


His Gln Ile Glu Lys Glu Phe Ser Glu Val Glu Gly Arg Ile Gln Asp 
            420                 425                 430         


Leu Glu Lys Tyr Val Glu Asp Thr Lys Ile Asp Leu Trp Ser Tyr Asn 
        435                 440                 445             


Ala Glu Leu Leu Val Ala Leu Glu Asn Gln His Thr Ile Asp Leu Thr 
    450                 455                 460                 


Asp Ser Glu Met Asn Lys Leu Phe Glu Lys Thr Lys Lys Gln Leu Arg 
465                 470                 475                 480 


Glu Asn Ala Glu Asp Met Gly Asn Gly Cys Phe Lys Ile Tyr His Lys 
                485                 490                 495     


Cys Asp Asn Ala Cys Met Gly Ser Ile Arg Asn Gly Thr Tyr Asp His 
            500                 505                 510         


Asn Val Tyr Arg Asp Glu Ala Leu Asn Asn Arg Phe Gln Ile Lys Gly 
        515                 520                 525             


Val Glu Leu Lys Ser Gly Tyr Lys Asp Trp Ile Leu Trp Ile Ser Phe 
    530                 535                 540                 


Ala Ile Ser Ser Leu Val Leu Leu Val Ala Leu Leu Gly Phe Ile Met 
545                 550                 555                 560 


Trp Ala Cys Gln Lys Gly Asn Ile Arg Cys Asn Ile Cys Ile 
                565                 570                 


<210>  70
<211>  48
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer H3Kansas(S136D).c

<400>  70
aaacggaaca gactcttctt gcataagggg atctaagagt agtttctt                    48


<210>  71
<211>  50
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer H3Kansas(S136D).r

<400>  71
caagaagagt ctgttccgtt ttgagtgact ccagcccaat tgaagctttc                  50


<210>  72
<211>  1725
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PDI-H3 A/Kansas/14/2017 Y98F, S136N

<400>  72
atggcgaaaa acgttgcgat tttcggctta ttgttttctc ttcttgtgtt ggttccttct       60

cagatcttcg cgcaaaaaat ccctggaaat gacaatagca cggcaacgct gtgccttggg      120

caccatgcag taccaaacgg aacgatagtg aaaacaatca cgaatgaccg aattgaagtt      180

actaatgcta ctgagctggt tcagaattcc tcaataggtg aaatatgcga cagtcctcat      240

cagatccttg atggagaaaa ctgcacacta atagatgctc tattgggaga ccctcagtgt      300

gatggctttc aaaataagaa atgggacctt tttgttgaac gaaacaaagc ctacagcaac      360

tgtttccctt atgatgtgcc ggattatgcc tcccttaggt cactagttgc ctcatccggc      420

acactggagt ttaacaatga aagcttcaat tgggctggag tcactcaaaa cggaacaaac      480

tcttcttgca taaggggatc taagagtagt ttctttagta gattaaattg gttgacccac      540

ttaaactcca aatacccagc attaaacgtg actatgccaa acaatgaaca atttgacaaa      600

ttgtacattt ggggggttca ccacccgggt acggacaagg accaaatcag cctgtatgca      660

caatcatcag gaagaatcac agtatctacc aaaagaagcc aacaagctgt aatcccgaat      720

atcggatcta gacccagaat aagggatatc cctagcagaa taagcatcta ttggacaata      780

gtaaaaccgg gagacatact tttgattaac agcacaggga atctaattgc tcctaggggt      840

tacttcaaaa tacgaagtgg gaaaagctca ataatgagat cagatgcacc cattggcaaa      900

tgcaagtctg aatgcatcac tccaaatgga agcattccca atgacaaacc attccaaaat      960

gtaaacagga tcacatacgg ggcctgtccc agatatgtta agcaaagcac tctgaaattg     1020

gcaacaggaa tgcgaaatgt accagagaga caaactagag gcatatttgg cgcaatagcg     1080

ggtttcatag aaaatggttg ggagggaatg gtggatggtt ggtacggctt caggcatcaa     1140

aattctgagg gaagaggaca agcagcagat ctcaaaagca ctcaagcagc aatcgatcaa     1200

atcaatggga agctggctcg agttatcggg aaaaccaacg agaaattcca tcagattgaa     1260

aaagaattct cagaagtaga agggagaatt caggaccttg agaaatatgt tgaggacaca     1320

aaaatagatc tctggtcata caacgcggag cttcttgttg ccctggagaa ccaacataca     1380

attgatctaa ctgactcaga aatgaacaaa ctgtttgaaa aaacaaagaa gcaactgagg     1440

gaaaatgctg aggatatggg caatggttgt ttcaaaatat accacaaatg tgacaatgcc     1500

tgcatgggat caatcagaaa tggaacttat gaccacaacg tatacaggga tgaagcatta     1560

aacaaccggt tccagatcaa gggagttgag ctgaagtcag ggtacaaaga ttggatccta     1620

tggatttcct ttgccatatc atcccttgta ctgttagttg ctttgttggg gttcatcatg     1680

tgggcctgcc aaaagggcaa cattaggtgc aacatttgca tttga                     1725


<210>  73
<211>  574
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  PDI-H3 A/Kansas/14/2017 Y98F, S136N

<400>  73

Met Ala Lys Asn Val Ala Ile Phe Gly Leu Leu Phe Ser Leu Leu Val 
1               5                   10                  15      


Leu Val Pro Ser Gln Ile Phe Ala Gln Lys Ile Pro Gly Asn Asp Asn 
            20                  25                  30          


Ser Thr Ala Thr Leu Cys Leu Gly His His Ala Val Pro Asn Gly Thr 
        35                  40                  45              


Ile Val Lys Thr Ile Thr Asn Asp Arg Ile Glu Val Thr Asn Ala Thr 
    50                  55                  60                  


Glu Leu Val Gln Asn Ser Ser Ile Gly Glu Ile Cys Asp Ser Pro His 
65                  70                  75                  80  


Gln Ile Leu Asp Gly Glu Asn Cys Thr Leu Ile Asp Ala Leu Leu Gly 
                85                  90                  95      


Asp Pro Gln Cys Asp Gly Phe Gln Asn Lys Lys Trp Asp Leu Phe Val 
            100                 105                 110         


Glu Arg Asn Lys Ala Tyr Ser Asn Cys Phe Pro Tyr Asp Val Pro Asp 
        115                 120                 125             


Tyr Ala Ser Leu Arg Ser Leu Val Ala Ser Ser Gly Thr Leu Glu Phe 
    130                 135                 140                 


Asn Asn Glu Ser Phe Asn Trp Ala Gly Val Thr Gln Asn Gly Thr Asn 
145                 150                 155                 160 


Ser Ser Cys Ile Arg Gly Ser Lys Ser Ser Phe Phe Ser Arg Leu Asn 
                165                 170                 175     


Trp Leu Thr His Leu Asn Ser Lys Tyr Pro Ala Leu Asn Val Thr Met 
            180                 185                 190         


Pro Asn Asn Glu Gln Phe Asp Lys Leu Tyr Ile Trp Gly Val His His 
        195                 200                 205             


Pro Gly Thr Asp Lys Asp Gln Ile Ser Leu Tyr Ala Gln Ser Ser Gly 
    210                 215                 220                 


Arg Ile Thr Val Ser Thr Lys Arg Ser Gln Gln Ala Val Ile Pro Asn 
225                 230                 235                 240 


Ile Gly Ser Arg Pro Arg Ile Arg Asp Ile Pro Ser Arg Ile Ser Ile 
                245                 250                 255     


Tyr Trp Thr Ile Val Lys Pro Gly Asp Ile Leu Leu Ile Asn Ser Thr 
            260                 265                 270         


Gly Asn Leu Ile Ala Pro Arg Gly Tyr Phe Lys Ile Arg Ser Gly Lys 
        275                 280                 285             


Ser Ser Ile Met Arg Ser Asp Ala Pro Ile Gly Lys Cys Lys Ser Glu 
    290                 295                 300                 


Cys Ile Thr Pro Asn Gly Ser Ile Pro Asn Asp Lys Pro Phe Gln Asn 
305                 310                 315                 320 


Val Asn Arg Ile Thr Tyr Gly Ala Cys Pro Arg Tyr Val Lys Gln Ser 
                325                 330                 335     


Thr Leu Lys Leu Ala Thr Gly Met Arg Asn Val Pro Glu Arg Gln Thr 
            340                 345                 350         


Arg Gly Ile Phe Gly Ala Ile Ala Gly Phe Ile Glu Asn Gly Trp Glu 
        355                 360                 365             


Gly Met Val Asp Gly Trp Tyr Gly Phe Arg His Gln Asn Ser Glu Gly 
    370                 375                 380                 


Arg Gly Gln Ala Ala Asp Leu Lys Ser Thr Gln Ala Ala Ile Asp Gln 
385                 390                 395                 400 


Ile Asn Gly Lys Leu Ala Arg Val Ile Gly Lys Thr Asn Glu Lys Phe 
                405                 410                 415     


His Gln Ile Glu Lys Glu Phe Ser Glu Val Glu Gly Arg Ile Gln Asp 
            420                 425                 430         


Leu Glu Lys Tyr Val Glu Asp Thr Lys Ile Asp Leu Trp Ser Tyr Asn 
        435                 440                 445             


Ala Glu Leu Leu Val Ala Leu Glu Asn Gln His Thr Ile Asp Leu Thr 
    450                 455                 460                 


Asp Ser Glu Met Asn Lys Leu Phe Glu Lys Thr Lys Lys Gln Leu Arg 
465                 470                 475                 480 


Glu Asn Ala Glu Asp Met Gly Asn Gly Cys Phe Lys Ile Tyr His Lys 
                485                 490                 495     


Cys Asp Asn Ala Cys Met Gly Ser Ile Arg Asn Gly Thr Tyr Asp His 
            500                 505                 510         


Asn Val Tyr Arg Asp Glu Ala Leu Asn Asn Arg Phe Gln Ile Lys Gly 
        515                 520                 525             


Val Glu Leu Lys Ser Gly Tyr Lys Asp Trp Ile Leu Trp Ile Ser Phe 
    530                 535                 540                 


Ala Ile Ser Ser Leu Val Leu Leu Val Ala Leu Leu Gly Phe Ile Met 
545                 550                 555                 560 


Trp Ala Cys Gln Lys Gly Asn Ile Arg Cys Asn Ile Cys Ile 
                565                 570                 


<210>  74
<211>  48
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer H3Kansas(S136N).c

<400>  74
aaacggaaca aactcttctt gcataagggg atctaagagt agtttctt                    48


<210>  75
<211>  50
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer H3Kansas(S136N).r

<400>  75
caagaagagt ttgttccgtt ttgagtgact ccagcccaat tgaagctttc                  50


<210>  76
<211>  1725
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PDI-H3 A/Kansas/14/2017 Y98F, S137N

<400>  76
atggcgaaaa acgttgcgat tttcggctta ttgttttctc ttcttgtgtt ggttccttct       60

cagatcttcg cgcaaaaaat ccctggaaat gacaatagca cggcaacgct gtgccttggg      120

caccatgcag taccaaacgg aacgatagtg aaaacaatca cgaatgaccg aattgaagtt      180

actaatgcta ctgagctggt tcagaattcc tcaataggtg aaatatgcga cagtcctcat      240

cagatccttg atggagaaaa ctgcacacta atagatgctc tattgggaga ccctcagtgt      300

gatggctttc aaaataagaa atgggacctt tttgttgaac gaaacaaagc ctacagcaac      360

tgtttccctt atgatgtgcc ggattatgcc tcccttaggt cactagttgc ctcatccggc      420

acactggagt ttaacaatga aagcttcaat tgggctggag tcactcaaaa cggaacaagt      480

aactcttgca taaggggatc taagagtagt ttctttagta gattaaattg gttgacccac      540

ttaaactcca aatacccagc attaaacgtg actatgccaa acaatgaaca atttgacaaa      600

ttgtacattt ggggggttca ccacccgggt acggacaagg accaaatcag cctgtatgca      660

caatcatcag gaagaatcac agtatctacc aaaagaagcc aacaagctgt aatcccgaat      720

atcggatcta gacccagaat aagggatatc cctagcagaa taagcatcta ttggacaata      780

gtaaaaccgg gagacatact tttgattaac agcacaggga atctaattgc tcctaggggt      840

tacttcaaaa tacgaagtgg gaaaagctca ataatgagat cagatgcacc cattggcaaa      900

tgcaagtctg aatgcatcac tccaaatgga agcattccca atgacaaacc attccaaaat      960

gtaaacagga tcacatacgg ggcctgtccc agatatgtta agcaaagcac tctgaaattg     1020

gcaacaggaa tgcgaaatgt accagagaga caaactagag gcatatttgg cgcaatagcg     1080

ggtttcatag aaaatggttg ggagggaatg gtggatggtt ggtacggctt caggcatcaa     1140

aattctgagg gaagaggaca agcagcagat ctcaaaagca ctcaagcagc aatcgatcaa     1200

atcaatggga agctggctcg agttatcggg aaaaccaacg agaaattcca tcagattgaa     1260

aaagaattct cagaagtaga agggagaatt caggaccttg agaaatatgt tgaggacaca     1320

aaaatagatc tctggtcata caacgcggag cttcttgttg ccctggagaa ccaacataca     1380

attgatctaa ctgactcaga aatgaacaaa ctgtttgaaa aaacaaagaa gcaactgagg     1440

gaaaatgctg aggatatggg caatggttgt ttcaaaatat accacaaatg tgacaatgcc     1500

tgcatgggat caatcagaaa tggaacttat gaccacaacg tatacaggga tgaagcatta     1560

aacaaccggt tccagatcaa gggagttgag ctgaagtcag ggtacaaaga ttggatccta     1620

tggatttcct ttgccatatc atcccttgta ctgttagttg ctttgttggg gttcatcatg     1680

tgggcctgcc aaaagggcaa cattaggtgc aacatttgca tttga                     1725


<210>  77
<211>  574
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  PDI-H3 A/Kansas/14/2017 Y98F, S137N

<400>  77

Met Ala Lys Asn Val Ala Ile Phe Gly Leu Leu Phe Ser Leu Leu Val 
1               5                   10                  15      


Leu Val Pro Ser Gln Ile Phe Ala Gln Lys Ile Pro Gly Asn Asp Asn 
            20                  25                  30          


Ser Thr Ala Thr Leu Cys Leu Gly His His Ala Val Pro Asn Gly Thr 
        35                  40                  45              


Ile Val Lys Thr Ile Thr Asn Asp Arg Ile Glu Val Thr Asn Ala Thr 
    50                  55                  60                  


Glu Leu Val Gln Asn Ser Ser Ile Gly Glu Ile Cys Asp Ser Pro His 
65                  70                  75                  80  


Gln Ile Leu Asp Gly Glu Asn Cys Thr Leu Ile Asp Ala Leu Leu Gly 
                85                  90                  95      


Asp Pro Gln Cys Asp Gly Phe Gln Asn Lys Lys Trp Asp Leu Phe Val 
            100                 105                 110         


Glu Arg Asn Lys Ala Tyr Ser Asn Cys Phe Pro Tyr Asp Val Pro Asp 
        115                 120                 125             


Tyr Ala Ser Leu Arg Ser Leu Val Ala Ser Ser Gly Thr Leu Glu Phe 
    130                 135                 140                 


Asn Asn Glu Ser Phe Asn Trp Ala Gly Val Thr Gln Asn Gly Thr Ser 
145                 150                 155                 160 


Asn Ser Cys Ile Arg Gly Ser Lys Ser Ser Phe Phe Ser Arg Leu Asn 
                165                 170                 175     


Trp Leu Thr His Leu Asn Ser Lys Tyr Pro Ala Leu Asn Val Thr Met 
            180                 185                 190         


Pro Asn Asn Glu Gln Phe Asp Lys Leu Tyr Ile Trp Gly Val His His 
        195                 200                 205             


Pro Gly Thr Asp Lys Asp Gln Ile Ser Leu Tyr Ala Gln Ser Ser Gly 
    210                 215                 220                 


Arg Ile Thr Val Ser Thr Lys Arg Ser Gln Gln Ala Val Ile Pro Asn 
225                 230                 235                 240 


Ile Gly Ser Arg Pro Arg Ile Arg Asp Ile Pro Ser Arg Ile Ser Ile 
                245                 250                 255     


Tyr Trp Thr Ile Val Lys Pro Gly Asp Ile Leu Leu Ile Asn Ser Thr 
            260                 265                 270         


Gly Asn Leu Ile Ala Pro Arg Gly Tyr Phe Lys Ile Arg Ser Gly Lys 
        275                 280                 285             


Ser Ser Ile Met Arg Ser Asp Ala Pro Ile Gly Lys Cys Lys Ser Glu 
    290                 295                 300                 


Cys Ile Thr Pro Asn Gly Ser Ile Pro Asn Asp Lys Pro Phe Gln Asn 
305                 310                 315                 320 


Val Asn Arg Ile Thr Tyr Gly Ala Cys Pro Arg Tyr Val Lys Gln Ser 
                325                 330                 335     


Thr Leu Lys Leu Ala Thr Gly Met Arg Asn Val Pro Glu Arg Gln Thr 
            340                 345                 350         


Arg Gly Ile Phe Gly Ala Ile Ala Gly Phe Ile Glu Asn Gly Trp Glu 
        355                 360                 365             


Gly Met Val Asp Gly Trp Tyr Gly Phe Arg His Gln Asn Ser Glu Gly 
    370                 375                 380                 


Arg Gly Gln Ala Ala Asp Leu Lys Ser Thr Gln Ala Ala Ile Asp Gln 
385                 390                 395                 400 


Ile Asn Gly Lys Leu Ala Arg Val Ile Gly Lys Thr Asn Glu Lys Phe 
                405                 410                 415     


His Gln Ile Glu Lys Glu Phe Ser Glu Val Glu Gly Arg Ile Gln Asp 
            420                 425                 430         


Leu Glu Lys Tyr Val Glu Asp Thr Lys Ile Asp Leu Trp Ser Tyr Asn 
        435                 440                 445             


Ala Glu Leu Leu Val Ala Leu Glu Asn Gln His Thr Ile Asp Leu Thr 
    450                 455                 460                 


Asp Ser Glu Met Asn Lys Leu Phe Glu Lys Thr Lys Lys Gln Leu Arg 
465                 470                 475                 480 


Glu Asn Ala Glu Asp Met Gly Asn Gly Cys Phe Lys Ile Tyr His Lys 
                485                 490                 495     


Cys Asp Asn Ala Cys Met Gly Ser Ile Arg Asn Gly Thr Tyr Asp His 
            500                 505                 510         


Asn Val Tyr Arg Asp Glu Ala Leu Asn Asn Arg Phe Gln Ile Lys Gly 
        515                 520                 525             


Val Glu Leu Lys Ser Gly Tyr Lys Asp Trp Ile Leu Trp Ile Ser Phe 
    530                 535                 540                 


Ala Ile Ser Ser Leu Val Leu Leu Val Ala Leu Leu Gly Phe Ile Met 
545                 550                 555                 560 


Trp Ala Cys Gln Lys Gly Asn Ile Arg Cys Asn Ile Cys Ile 
                565                 570                 


<210>  78
<211>  51
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer H3Kansas(S137N).c

<400>  78
cggaacaagt aactcttgca taaggggatc taagagtagt ttctttagta g                51


<210>  79
<211>  55
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer H3Kansas(S137N).r

<400>  79
atgcaagagt tacttgttcc gttttgagtg actccagccc aattgaagct ttcat            55


<210>  80
<211>  1725
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PDI-H3 A/Kansas/14/2017 Y98F, D190G

<400>  80
atggcgaaaa acgttgcgat tttcggctta ttgttttctc ttcttgtgtt ggttccttct       60

cagatcttcg cgcaaaaaat ccctggaaat gacaatagca cggcaacgct gtgccttggg      120

caccatgcag taccaaacgg aacgatagtg aaaacaatca cgaatgaccg aattgaagtt      180

actaatgcta ctgagctggt tcagaattcc tcaataggtg aaatatgcga cagtcctcat      240

cagatccttg atggagaaaa ctgcacacta atagatgctc tattgggaga ccctcagtgt      300

gatggctttc aaaataagaa atgggacctt tttgttgaac gaaacaaagc ctacagcaac      360

tgtttccctt atgatgtgcc ggattatgcc tcccttaggt cactagttgc ctcatccggc      420

acactggagt ttaacaatga aagcttcaat tgggctggag tcactcaaaa cggaacaagt      480

tcttcttgca taaggggatc taagagtagt ttctttagta gattaaattg gttgacccac      540

ttaaactcca aatacccagc attaaacgtg actatgccaa acaatgaaca atttgacaaa      600

ttgtacattt ggggggttca ccacccgggt acggacaagg gccaaatcag cctgtatgca      660

caatcatcag gaagaatcac agtatctacc aaaagaagcc aacaagctgt aatcccgaat      720

atcggatcta gacccagaat aagggatatc cctagcagaa taagcatcta ttggacaata      780

gtaaaaccgg gagacatact tttgattaac agcacaggga atctaattgc tcctaggggt      840

tacttcaaaa tacgaagtgg gaaaagctca ataatgagat cagatgcacc cattggcaaa      900

tgcaagtctg aatgcatcac tccaaatgga agcattccca atgacaaacc attccaaaat      960

gtaaacagga tcacatacgg ggcctgtccc agatatgtta agcaaagcac tctgaaattg     1020

gcaacaggaa tgcgaaatgt accagagaga caaactagag gcatatttgg cgcaatagcg     1080

ggtttcatag aaaatggttg ggagggaatg gtggatggtt ggtacggctt caggcatcaa     1140

aattctgagg gaagaggaca agcagcagat ctcaaaagca ctcaagcagc aatcgatcaa     1200

atcaatggga agctggctcg agttatcggg aaaaccaacg agaaattcca tcagattgaa     1260

aaagaattct cagaagtaga agggagaatt caggaccttg agaaatatgt tgaggacaca     1320

aaaatagatc tctggtcata caacgcggag cttcttgttg ccctggagaa ccaacataca     1380

attgatctaa ctgactcaga aatgaacaaa ctgtttgaaa aaacaaagaa gcaactgagg     1440

gaaaatgctg aggatatggg caatggttgt ttcaaaatat accacaaatg tgacaatgcc     1500

tgcatgggat caatcagaaa tggaacttat gaccacaacg tatacaggga tgaagcatta     1560

aacaaccggt tccagatcaa gggagttgag ctgaagtcag ggtacaaaga ttggatccta     1620

tggatttcct ttgccatatc atcccttgta ctgttagttg ctttgttggg gttcatcatg     1680

tgggcctgcc aaaagggcaa cattaggtgc aacatttgca tttga                     1725


<210>  81
<211>  574
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  PDI-H3 A/Kansas/14/2017 Y98F, D190G

<400>  81

Met Ala Lys Asn Val Ala Ile Phe Gly Leu Leu Phe Ser Leu Leu Val 
1               5                   10                  15      


Leu Val Pro Ser Gln Ile Phe Ala Gln Lys Ile Pro Gly Asn Asp Asn 
            20                  25                  30          


Ser Thr Ala Thr Leu Cys Leu Gly His His Ala Val Pro Asn Gly Thr 
        35                  40                  45              


Ile Val Lys Thr Ile Thr Asn Asp Arg Ile Glu Val Thr Asn Ala Thr 
    50                  55                  60                  


Glu Leu Val Gln Asn Ser Ser Ile Gly Glu Ile Cys Asp Ser Pro His 
65                  70                  75                  80  


Gln Ile Leu Asp Gly Glu Asn Cys Thr Leu Ile Asp Ala Leu Leu Gly 
                85                  90                  95      


Asp Pro Gln Cys Asp Gly Phe Gln Asn Lys Lys Trp Asp Leu Phe Val 
            100                 105                 110         


Glu Arg Asn Lys Ala Tyr Ser Asn Cys Phe Pro Tyr Asp Val Pro Asp 
        115                 120                 125             


Tyr Ala Ser Leu Arg Ser Leu Val Ala Ser Ser Gly Thr Leu Glu Phe 
    130                 135                 140                 


Asn Asn Glu Ser Phe Asn Trp Ala Gly Val Thr Gln Asn Gly Thr Ser 
145                 150                 155                 160 


Ser Ser Cys Ile Arg Gly Ser Lys Ser Ser Phe Phe Ser Arg Leu Asn 
                165                 170                 175     


Trp Leu Thr His Leu Asn Ser Lys Tyr Pro Ala Leu Asn Val Thr Met 
            180                 185                 190         


Pro Asn Asn Glu Gln Phe Asp Lys Leu Tyr Ile Trp Gly Val His His 
        195                 200                 205             


Pro Gly Thr Asp Lys Gly Gln Ile Ser Leu Tyr Ala Gln Ser Ser Gly 
    210                 215                 220                 


Arg Ile Thr Val Ser Thr Lys Arg Ser Gln Gln Ala Val Ile Pro Asn 
225                 230                 235                 240 


Ile Gly Ser Arg Pro Arg Ile Arg Asp Ile Pro Ser Arg Ile Ser Ile 
                245                 250                 255     


Tyr Trp Thr Ile Val Lys Pro Gly Asp Ile Leu Leu Ile Asn Ser Thr 
            260                 265                 270         


Gly Asn Leu Ile Ala Pro Arg Gly Tyr Phe Lys Ile Arg Ser Gly Lys 
        275                 280                 285             


Ser Ser Ile Met Arg Ser Asp Ala Pro Ile Gly Lys Cys Lys Ser Glu 
    290                 295                 300                 


Cys Ile Thr Pro Asn Gly Ser Ile Pro Asn Asp Lys Pro Phe Gln Asn 
305                 310                 315                 320 


Val Asn Arg Ile Thr Tyr Gly Ala Cys Pro Arg Tyr Val Lys Gln Ser 
                325                 330                 335     


Thr Leu Lys Leu Ala Thr Gly Met Arg Asn Val Pro Glu Arg Gln Thr 
            340                 345                 350         


Arg Gly Ile Phe Gly Ala Ile Ala Gly Phe Ile Glu Asn Gly Trp Glu 
        355                 360                 365             


Gly Met Val Asp Gly Trp Tyr Gly Phe Arg His Gln Asn Ser Glu Gly 
    370                 375                 380                 


Arg Gly Gln Ala Ala Asp Leu Lys Ser Thr Gln Ala Ala Ile Asp Gln 
385                 390                 395                 400 


Ile Asn Gly Lys Leu Ala Arg Val Ile Gly Lys Thr Asn Glu Lys Phe 
                405                 410                 415     


His Gln Ile Glu Lys Glu Phe Ser Glu Val Glu Gly Arg Ile Gln Asp 
            420                 425                 430         


Leu Glu Lys Tyr Val Glu Asp Thr Lys Ile Asp Leu Trp Ser Tyr Asn 
        435                 440                 445             


Ala Glu Leu Leu Val Ala Leu Glu Asn Gln His Thr Ile Asp Leu Thr 
    450                 455                 460                 


Asp Ser Glu Met Asn Lys Leu Phe Glu Lys Thr Lys Lys Gln Leu Arg 
465                 470                 475                 480 


Glu Asn Ala Glu Asp Met Gly Asn Gly Cys Phe Lys Ile Tyr His Lys 
                485                 490                 495     


Cys Asp Asn Ala Cys Met Gly Ser Ile Arg Asn Gly Thr Tyr Asp His 
            500                 505                 510         


Asn Val Tyr Arg Asp Glu Ala Leu Asn Asn Arg Phe Gln Ile Lys Gly 
        515                 520                 525             


Val Glu Leu Lys Ser Gly Tyr Lys Asp Trp Ile Leu Trp Ile Ser Phe 
    530                 535                 540                 


Ala Ile Ser Ser Leu Val Leu Leu Val Ala Leu Leu Gly Phe Ile Met 
545                 550                 555                 560 


Trp Ala Cys Gln Lys Gly Asn Ile Arg Cys Asn Ile Cys Ile 
                565                 570                 


<210>  82
<211>  49
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer H3Kansas(D190G).c

<400>  82
tacggacaag ggccaaatca gcctgtatgc acaatcatca ggaagaatc                   49


<210>  83
<211>  48
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer H3Kansas(D190G).r

<400>  83
ctgatttggc ccttgtccgt acccgggtgg tgaacccccc aaatgtac                    48


<210>  84
<211>  1725
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PDI-H3 A/Kansas/14/2017 Y98F, D190K

<400>  84
atggcgaaaa acgttgcgat tttcggctta ttgttttctc ttcttgtgtt ggttccttct       60

cagatcttcg cgcaaaaaat ccctggaaat gacaatagca cggcaacgct gtgccttggg      120

caccatgcag taccaaacgg aacgatagtg aaaacaatca cgaatgaccg aattgaagtt      180

actaatgcta ctgagctggt tcagaattcc tcaataggtg aaatatgcga cagtcctcat      240

cagatccttg atggagaaaa ctgcacacta atagatgctc tattgggaga ccctcagtgt      300

gatggctttc aaaataagaa atgggacctt tttgttgaac gaaacaaagc ctacagcaac      360

tgtttccctt atgatgtgcc ggattatgcc tcccttaggt cactagttgc ctcatccggc      420

acactggagt ttaacaatga aagcttcaat tgggctggag tcactcaaaa cggaacaagt      480

tcttcttgca taaggggatc taagagtagt ttctttagta gattaaattg gttgacccac      540

ttaaactcca aatacccagc attaaacgtg actatgccaa acaatgaaca atttgacaaa      600

ttgtacattt ggggggttca ccacccgggt acggacaaga agcaaatcag cctgtatgca      660

caatcatcag gaagaatcac agtatctacc aaaagaagcc aacaagctgt aatcccgaat      720

atcggatcta gacccagaat aagggatatc cctagcagaa taagcatcta ttggacaata      780

gtaaaaccgg gagacatact tttgattaac agcacaggga atctaattgc tcctaggggt      840

tacttcaaaa tacgaagtgg gaaaagctca ataatgagat cagatgcacc cattggcaaa      900

tgcaagtctg aatgcatcac tccaaatgga agcattccca atgacaaacc attccaaaat      960

gtaaacagga tcacatacgg ggcctgtccc agatatgtta agcaaagcac tctgaaattg     1020

gcaacaggaa tgcgaaatgt accagagaga caaactagag gcatatttgg cgcaatagcg     1080

ggtttcatag aaaatggttg ggagggaatg gtggatggtt ggtacggctt caggcatcaa     1140

aattctgagg gaagaggaca agcagcagat ctcaaaagca ctcaagcagc aatcgatcaa     1200

atcaatggga agctggctcg agttatcggg aaaaccaacg agaaattcca tcagattgaa     1260

aaagaattct cagaagtaga agggagaatt caggaccttg agaaatatgt tgaggacaca     1320

aaaatagatc tctggtcata caacgcggag cttcttgttg ccctggagaa ccaacataca     1380

attgatctaa ctgactcaga aatgaacaaa ctgtttgaaa aaacaaagaa gcaactgagg     1440

gaaaatgctg aggatatggg caatggttgt ttcaaaatat accacaaatg tgacaatgcc     1500

tgcatgggat caatcagaaa tggaacttat gaccacaacg tatacaggga tgaagcatta     1560

aacaaccggt tccagatcaa gggagttgag ctgaagtcag ggtacaaaga ttggatccta     1620

tggatttcct ttgccatatc atcccttgta ctgttagttg ctttgttggg gttcatcatg     1680

tgggcctgcc aaaagggcaa cattaggtgc aacatttgca tttga                     1725


<210>  85
<211>  574
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  PDI-H3 A/Kansas/14/2017 Y98F, D190K

<400>  85

Met Ala Lys Asn Val Ala Ile Phe Gly Leu Leu Phe Ser Leu Leu Val 
1               5                   10                  15      


Leu Val Pro Ser Gln Ile Phe Ala Gln Lys Ile Pro Gly Asn Asp Asn 
            20                  25                  30          


Ser Thr Ala Thr Leu Cys Leu Gly His His Ala Val Pro Asn Gly Thr 
        35                  40                  45              


Ile Val Lys Thr Ile Thr Asn Asp Arg Ile Glu Val Thr Asn Ala Thr 
    50                  55                  60                  


Glu Leu Val Gln Asn Ser Ser Ile Gly Glu Ile Cys Asp Ser Pro His 
65                  70                  75                  80  


Gln Ile Leu Asp Gly Glu Asn Cys Thr Leu Ile Asp Ala Leu Leu Gly 
                85                  90                  95      


Asp Pro Gln Cys Asp Gly Phe Gln Asn Lys Lys Trp Asp Leu Phe Val 
            100                 105                 110         


Glu Arg Asn Lys Ala Tyr Ser Asn Cys Phe Pro Tyr Asp Val Pro Asp 
        115                 120                 125             


Tyr Ala Ser Leu Arg Ser Leu Val Ala Ser Ser Gly Thr Leu Glu Phe 
    130                 135                 140                 


Asn Asn Glu Ser Phe Asn Trp Ala Gly Val Thr Gln Asn Gly Thr Ser 
145                 150                 155                 160 


Ser Ser Cys Ile Arg Gly Ser Lys Ser Ser Phe Phe Ser Arg Leu Asn 
                165                 170                 175     


Trp Leu Thr His Leu Asn Ser Lys Tyr Pro Ala Leu Asn Val Thr Met 
            180                 185                 190         


Pro Asn Asn Glu Gln Phe Asp Lys Leu Tyr Ile Trp Gly Val His His 
        195                 200                 205             


Pro Gly Thr Asp Lys Lys Gln Ile Ser Leu Tyr Ala Gln Ser Ser Gly 
    210                 215                 220                 


Arg Ile Thr Val Ser Thr Lys Arg Ser Gln Gln Ala Val Ile Pro Asn 
225                 230                 235                 240 


Ile Gly Ser Arg Pro Arg Ile Arg Asp Ile Pro Ser Arg Ile Ser Ile 
                245                 250                 255     


Tyr Trp Thr Ile Val Lys Pro Gly Asp Ile Leu Leu Ile Asn Ser Thr 
            260                 265                 270         


Gly Asn Leu Ile Ala Pro Arg Gly Tyr Phe Lys Ile Arg Ser Gly Lys 
        275                 280                 285             


Ser Ser Ile Met Arg Ser Asp Ala Pro Ile Gly Lys Cys Lys Ser Glu 
    290                 295                 300                 


Cys Ile Thr Pro Asn Gly Ser Ile Pro Asn Asp Lys Pro Phe Gln Asn 
305                 310                 315                 320 


Val Asn Arg Ile Thr Tyr Gly Ala Cys Pro Arg Tyr Val Lys Gln Ser 
                325                 330                 335     


Thr Leu Lys Leu Ala Thr Gly Met Arg Asn Val Pro Glu Arg Gln Thr 
            340                 345                 350         


Arg Gly Ile Phe Gly Ala Ile Ala Gly Phe Ile Glu Asn Gly Trp Glu 
        355                 360                 365             


Gly Met Val Asp Gly Trp Tyr Gly Phe Arg His Gln Asn Ser Glu Gly 
    370                 375                 380                 


Arg Gly Gln Ala Ala Asp Leu Lys Ser Thr Gln Ala Ala Ile Asp Gln 
385                 390                 395                 400 


Ile Asn Gly Lys Leu Ala Arg Val Ile Gly Lys Thr Asn Glu Lys Phe 
                405                 410                 415     


His Gln Ile Glu Lys Glu Phe Ser Glu Val Glu Gly Arg Ile Gln Asp 
            420                 425                 430         


Leu Glu Lys Tyr Val Glu Asp Thr Lys Ile Asp Leu Trp Ser Tyr Asn 
        435                 440                 445             


Ala Glu Leu Leu Val Ala Leu Glu Asn Gln His Thr Ile Asp Leu Thr 
    450                 455                 460                 


Asp Ser Glu Met Asn Lys Leu Phe Glu Lys Thr Lys Lys Gln Leu Arg 
465                 470                 475                 480 


Glu Asn Ala Glu Asp Met Gly Asn Gly Cys Phe Lys Ile Tyr His Lys 
                485                 490                 495     


Cys Asp Asn Ala Cys Met Gly Ser Ile Arg Asn Gly Thr Tyr Asp His 
            500                 505                 510         


Asn Val Tyr Arg Asp Glu Ala Leu Asn Asn Arg Phe Gln Ile Lys Gly 
        515                 520                 525             


Val Glu Leu Lys Ser Gly Tyr Lys Asp Trp Ile Leu Trp Ile Ser Phe 
    530                 535                 540                 


Ala Ile Ser Ser Leu Val Leu Leu Val Ala Leu Leu Gly Phe Ile Met 
545                 550                 555                 560 


Trp Ala Cys Gln Lys Gly Asn Ile Arg Cys Asn Ile Cys Ile 
                565                 570                 


<210>  86
<211>  49
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer H3Kansas(D190K).c

<400>  86
tacggacaag aagcaaatca gcctgtatgc acaatcatca ggaagaatc                   49


<210>  87
<211>  48
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer H3Kansas(D190K).r

<400>  87
ctgatttgct tcttgtccgt acccgggtgg tgaacccccc aaatgtac                    48


<210>  88
<211>  1725
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PDI-H3 A/Kansas/14/2017 Y98F, R222W

<400>  88
atggcgaaaa acgttgcgat tttcggctta ttgttttctc ttcttgtgtt ggttccttct       60

cagatcttcg cgcaaaaaat ccctggaaat gacaatagca cggcaacgct gtgccttggg      120

caccatgcag taccaaacgg aacgatagtg aaaacaatca cgaatgaccg aattgaagtt      180

actaatgcta ctgagctggt tcagaattcc tcaataggtg aaatatgcga cagtcctcat      240

cagatccttg atggagaaaa ctgcacacta atagatgctc tattgggaga ccctcagtgt      300

gatggctttc aaaataagaa atgggacctt tttgttgaac gaaacaaagc ctacagcaac      360

tgtttccctt atgatgtgcc ggattatgcc tcccttaggt cactagttgc ctcatccggc      420

acactggagt ttaacaatga aagcttcaat tgggctggag tcactcaaaa cggaacaagt      480

tcttcttgca taaggggatc taagagtagt ttctttagta gattaaattg gttgacccac      540

ttaaactcca aatacccagc attaaacgtg actatgccaa acaatgaaca atttgacaaa      600

ttgtacattt ggggggttca ccacccgggt acggacaagg accaaatcag cctgtatgca      660

caatcatcag gaagaatcac agtatctacc aaaagaagcc aacaagctgt aatcccgaat      720

atcggatcta gaccctggat aagggatatc cctagcagaa taagcatcta ttggacaata      780

gtaaaaccgg gagacatact tttgattaac agcacaggga atctaattgc tcctaggggt      840

tacttcaaaa tacgaagtgg gaaaagctca ataatgagat cagatgcacc cattggcaaa      900

tgcaagtctg aatgcatcac tccaaatgga agcattccca atgacaaacc attccaaaat      960

gtaaacagga tcacatacgg ggcctgtccc agatatgtta agcaaagcac tctgaaattg     1020

gcaacaggaa tgcgaaatgt accagagaga caaactagag gcatatttgg cgcaatagcg     1080

ggtttcatag aaaatggttg ggagggaatg gtggatggtt ggtacggctt caggcatcaa     1140

aattctgagg gaagaggaca agcagcagat ctcaaaagca ctcaagcagc aatcgatcaa     1200

atcaatggga agctggctcg agttatcggg aaaaccaacg agaaattcca tcagattgaa     1260

aaagaattct cagaagtaga agggagaatt caggaccttg agaaatatgt tgaggacaca     1320

aaaatagatc tctggtcata caacgcggag cttcttgttg ccctggagaa ccaacataca     1380

attgatctaa ctgactcaga aatgaacaaa ctgtttgaaa aaacaaagaa gcaactgagg     1440

gaaaatgctg aggatatggg caatggttgt ttcaaaatat accacaaatg tgacaatgcc     1500

tgcatgggat caatcagaaa tggaacttat gaccacaacg tatacaggga tgaagcatta     1560

aacaaccggt tccagatcaa gggagttgag ctgaagtcag ggtacaaaga ttggatccta     1620

tggatttcct ttgccatatc atcccttgta ctgttagttg ctttgttggg gttcatcatg     1680

tgggcctgcc aaaagggcaa cattaggtgc aacatttgca tttga                     1725


<210>  89
<211>  574
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  PDI-H3 A/Kansas/14/2017 Y98F, R222W

<400>  89

Met Ala Lys Asn Val Ala Ile Phe Gly Leu Leu Phe Ser Leu Leu Val 
1               5                   10                  15      


Leu Val Pro Ser Gln Ile Phe Ala Gln Lys Ile Pro Gly Asn Asp Asn 
            20                  25                  30          


Ser Thr Ala Thr Leu Cys Leu Gly His His Ala Val Pro Asn Gly Thr 
        35                  40                  45              


Ile Val Lys Thr Ile Thr Asn Asp Arg Ile Glu Val Thr Asn Ala Thr 
    50                  55                  60                  


Glu Leu Val Gln Asn Ser Ser Ile Gly Glu Ile Cys Asp Ser Pro His 
65                  70                  75                  80  


Gln Ile Leu Asp Gly Glu Asn Cys Thr Leu Ile Asp Ala Leu Leu Gly 
                85                  90                  95      


Asp Pro Gln Cys Asp Gly Phe Gln Asn Lys Lys Trp Asp Leu Phe Val 
            100                 105                 110         


Glu Arg Asn Lys Ala Tyr Ser Asn Cys Phe Pro Tyr Asp Val Pro Asp 
        115                 120                 125             


Tyr Ala Ser Leu Arg Ser Leu Val Ala Ser Ser Gly Thr Leu Glu Phe 
    130                 135                 140                 


Asn Asn Glu Ser Phe Asn Trp Ala Gly Val Thr Gln Asn Gly Thr Ser 
145                 150                 155                 160 


Ser Ser Cys Ile Arg Gly Ser Lys Ser Ser Phe Phe Ser Arg Leu Asn 
                165                 170                 175     


Trp Leu Thr His Leu Asn Ser Lys Tyr Pro Ala Leu Asn Val Thr Met 
            180                 185                 190         


Pro Asn Asn Glu Gln Phe Asp Lys Leu Tyr Ile Trp Gly Val His His 
        195                 200                 205             


Pro Gly Thr Asp Lys Asp Gln Ile Ser Leu Tyr Ala Gln Ser Ser Gly 
    210                 215                 220                 


Arg Ile Thr Val Ser Thr Lys Arg Ser Gln Gln Ala Val Ile Pro Asn 
225                 230                 235                 240 


Ile Gly Ser Arg Pro Trp Ile Arg Asp Ile Pro Ser Arg Ile Ser Ile 
                245                 250                 255     


Tyr Trp Thr Ile Val Lys Pro Gly Asp Ile Leu Leu Ile Asn Ser Thr 
            260                 265                 270         


Gly Asn Leu Ile Ala Pro Arg Gly Tyr Phe Lys Ile Arg Ser Gly Lys 
        275                 280                 285             


Ser Ser Ile Met Arg Ser Asp Ala Pro Ile Gly Lys Cys Lys Ser Glu 
    290                 295                 300                 


Cys Ile Thr Pro Asn Gly Ser Ile Pro Asn Asp Lys Pro Phe Gln Asn 
305                 310                 315                 320 


Val Asn Arg Ile Thr Tyr Gly Ala Cys Pro Arg Tyr Val Lys Gln Ser 
                325                 330                 335     


Thr Leu Lys Leu Ala Thr Gly Met Arg Asn Val Pro Glu Arg Gln Thr 
            340                 345                 350         


Arg Gly Ile Phe Gly Ala Ile Ala Gly Phe Ile Glu Asn Gly Trp Glu 
        355                 360                 365             


Gly Met Val Asp Gly Trp Tyr Gly Phe Arg His Gln Asn Ser Glu Gly 
    370                 375                 380                 


Arg Gly Gln Ala Ala Asp Leu Lys Ser Thr Gln Ala Ala Ile Asp Gln 
385                 390                 395                 400 


Ile Asn Gly Lys Leu Ala Arg Val Ile Gly Lys Thr Asn Glu Lys Phe 
                405                 410                 415     


His Gln Ile Glu Lys Glu Phe Ser Glu Val Glu Gly Arg Ile Gln Asp 
            420                 425                 430         


Leu Glu Lys Tyr Val Glu Asp Thr Lys Ile Asp Leu Trp Ser Tyr Asn 
        435                 440                 445             


Ala Glu Leu Leu Val Ala Leu Glu Asn Gln His Thr Ile Asp Leu Thr 
    450                 455                 460                 


Asp Ser Glu Met Asn Lys Leu Phe Glu Lys Thr Lys Lys Gln Leu Arg 
465                 470                 475                 480 


Glu Asn Ala Glu Asp Met Gly Asn Gly Cys Phe Lys Ile Tyr His Lys 
                485                 490                 495     


Cys Asp Asn Ala Cys Met Gly Ser Ile Arg Asn Gly Thr Tyr Asp His 
            500                 505                 510         


Asn Val Tyr Arg Asp Glu Ala Leu Asn Asn Arg Phe Gln Ile Lys Gly 
        515                 520                 525             


Val Glu Leu Lys Ser Gly Tyr Lys Asp Trp Ile Leu Trp Ile Ser Phe 
    530                 535                 540                 


Ala Ile Ser Ser Leu Val Leu Leu Val Ala Leu Leu Gly Phe Ile Met 
545                 550                 555                 560 


Trp Ala Cys Gln Lys Gly Asn Ile Arg Cys Asn Ile Cys Ile 
                565                 570                 


<210>  90
<211>  50
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer H3Kansas(R222W).c

<400>  90
atctagaccc tggataaggg atatccctag cagaataagc atctattgga                  50


<210>  91
<211>  49
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer H3Kansas(R222W).r

<400>  91
tcccttatcc agggtctaga tccgatattc gggattacag cttgttggc                   49


<210>  92
<211>  1725
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PDI-H3 A/Kansas/14/2017 Y98F, S228N

<400>  92
atggcgaaaa acgttgcgat tttcggctta ttgttttctc ttcttgtgtt ggttccttct       60

cagatcttcg cgcaaaaaat ccctggaaat gacaatagca cggcaacgct gtgccttggg      120

caccatgcag taccaaacgg aacgatagtg aaaacaatca cgaatgaccg aattgaagtt      180

actaatgcta ctgagctggt tcagaattcc tcaataggtg aaatatgcga cagtcctcat      240

cagatccttg atggagaaaa ctgcacacta atagatgctc tattgggaga ccctcagtgt      300

gatggctttc aaaataagaa atgggacctt tttgttgaac gaaacaaagc ctacagcaac      360

tgtttccctt atgatgtgcc ggattatgcc tcccttaggt cactagttgc ctcatccggc      420

acactggagt ttaacaatga aagcttcaat tgggctggag tcactcaaaa cggaacaagt      480

tcttcttgca taaggggatc taagagtagt ttctttagta gattaaattg gttgacccac      540

ttaaactcca aatacccagc attaaacgtg actatgccaa acaatgaaca atttgacaaa      600

ttgtacattt ggggggttca ccacccgggt acggacaagg accaaatcag cctgtatgca      660

caatcatcag gaagaatcac agtatctacc aaaagaagcc aacaagctgt aatcccgaat      720

atcggatcta gacccagaat aagggatatc cctaacagaa taagcatcta ttggacaata      780

gtaaaaccgg gagacatact tttgattaac agcacaggga atctaattgc tcctaggggt      840

tacttcaaaa tacgaagtgg gaaaagctca ataatgagat cagatgcacc cattggcaaa      900

tgcaagtctg aatgcatcac tccaaatgga agcattccca atgacaaacc attccaaaat      960

gtaaacagga tcacatacgg ggcctgtccc agatatgtta agcaaagcac tctgaaattg     1020

gcaacaggaa tgcgaaatgt accagagaga caaactagag gcatatttgg cgcaatagcg     1080

ggtttcatag aaaatggttg ggagggaatg gtggatggtt ggtacggctt caggcatcaa     1140

aattctgagg gaagaggaca agcagcagat ctcaaaagca ctcaagcagc aatcgatcaa     1200

atcaatggga agctggctcg agttatcggg aaaaccaacg agaaattcca tcagattgaa     1260

aaagaattct cagaagtaga agggagaatt caggaccttg agaaatatgt tgaggacaca     1320

aaaatagatc tctggtcata caacgcggag cttcttgttg ccctggagaa ccaacataca     1380

attgatctaa ctgactcaga aatgaacaaa ctgtttgaaa aaacaaagaa gcaactgagg     1440

gaaaatgctg aggatatggg caatggttgt ttcaaaatat accacaaatg tgacaatgcc     1500

tgcatgggat caatcagaaa tggaacttat gaccacaacg tatacaggga tgaagcatta     1560

aacaaccggt tccagatcaa gggagttgag ctgaagtcag ggtacaaaga ttggatccta     1620

tggatttcct ttgccatatc atcccttgta ctgttagttg ctttgttggg gttcatcatg     1680

tgggcctgcc aaaagggcaa cattaggtgc aacatttgca tttga                     1725


<210>  93
<211>  574
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  PDI-H3 A/Kansas/14/2017 Y98F, S228N

<400>  93

Met Ala Lys Asn Val Ala Ile Phe Gly Leu Leu Phe Ser Leu Leu Val 
1               5                   10                  15      


Leu Val Pro Ser Gln Ile Phe Ala Gln Lys Ile Pro Gly Asn Asp Asn 
            20                  25                  30          


Ser Thr Ala Thr Leu Cys Leu Gly His His Ala Val Pro Asn Gly Thr 
        35                  40                  45              


Ile Val Lys Thr Ile Thr Asn Asp Arg Ile Glu Val Thr Asn Ala Thr 
    50                  55                  60                  


Glu Leu Val Gln Asn Ser Ser Ile Gly Glu Ile Cys Asp Ser Pro His 
65                  70                  75                  80  


Gln Ile Leu Asp Gly Glu Asn Cys Thr Leu Ile Asp Ala Leu Leu Gly 
                85                  90                  95      


Asp Pro Gln Cys Asp Gly Phe Gln Asn Lys Lys Trp Asp Leu Phe Val 
            100                 105                 110         


Glu Arg Asn Lys Ala Tyr Ser Asn Cys Phe Pro Tyr Asp Val Pro Asp 
        115                 120                 125             


Tyr Ala Ser Leu Arg Ser Leu Val Ala Ser Ser Gly Thr Leu Glu Phe 
    130                 135                 140                 


Asn Asn Glu Ser Phe Asn Trp Ala Gly Val Thr Gln Asn Gly Thr Ser 
145                 150                 155                 160 


Ser Ser Cys Ile Arg Gly Ser Lys Ser Ser Phe Phe Ser Arg Leu Asn 
                165                 170                 175     


Trp Leu Thr His Leu Asn Ser Lys Tyr Pro Ala Leu Asn Val Thr Met 
            180                 185                 190         


Pro Asn Asn Glu Gln Phe Asp Lys Leu Tyr Ile Trp Gly Val His His 
        195                 200                 205             


Pro Gly Thr Asp Lys Asp Gln Ile Ser Leu Tyr Ala Gln Ser Ser Gly 
    210                 215                 220                 


Arg Ile Thr Val Ser Thr Lys Arg Ser Gln Gln Ala Val Ile Pro Asn 
225                 230                 235                 240 


Ile Gly Ser Arg Pro Arg Ile Arg Asp Ile Pro Asn Arg Ile Ser Ile 
                245                 250                 255     


Tyr Trp Thr Ile Val Lys Pro Gly Asp Ile Leu Leu Ile Asn Ser Thr 
            260                 265                 270         


Gly Asn Leu Ile Ala Pro Arg Gly Tyr Phe Lys Ile Arg Ser Gly Lys 
        275                 280                 285             


Ser Ser Ile Met Arg Ser Asp Ala Pro Ile Gly Lys Cys Lys Ser Glu 
    290                 295                 300                 


Cys Ile Thr Pro Asn Gly Ser Ile Pro Asn Asp Lys Pro Phe Gln Asn 
305                 310                 315                 320 


Val Asn Arg Ile Thr Tyr Gly Ala Cys Pro Arg Tyr Val Lys Gln Ser 
                325                 330                 335     


Thr Leu Lys Leu Ala Thr Gly Met Arg Asn Val Pro Glu Arg Gln Thr 
            340                 345                 350         


Arg Gly Ile Phe Gly Ala Ile Ala Gly Phe Ile Glu Asn Gly Trp Glu 
        355                 360                 365             


Gly Met Val Asp Gly Trp Tyr Gly Phe Arg His Gln Asn Ser Glu Gly 
    370                 375                 380                 


Arg Gly Gln Ala Ala Asp Leu Lys Ser Thr Gln Ala Ala Ile Asp Gln 
385                 390                 395                 400 


Ile Asn Gly Lys Leu Ala Arg Val Ile Gly Lys Thr Asn Glu Lys Phe 
                405                 410                 415     


His Gln Ile Glu Lys Glu Phe Ser Glu Val Glu Gly Arg Ile Gln Asp 
            420                 425                 430         


Leu Glu Lys Tyr Val Glu Asp Thr Lys Ile Asp Leu Trp Ser Tyr Asn 
        435                 440                 445             


Ala Glu Leu Leu Val Ala Leu Glu Asn Gln His Thr Ile Asp Leu Thr 
    450                 455                 460                 


Asp Ser Glu Met Asn Lys Leu Phe Glu Lys Thr Lys Lys Gln Leu Arg 
465                 470                 475                 480 


Glu Asn Ala Glu Asp Met Gly Asn Gly Cys Phe Lys Ile Tyr His Lys 
                485                 490                 495     


Cys Asp Asn Ala Cys Met Gly Ser Ile Arg Asn Gly Thr Tyr Asp His 
            500                 505                 510         


Asn Val Tyr Arg Asp Glu Ala Leu Asn Asn Arg Phe Gln Ile Lys Gly 
        515                 520                 525             


Val Glu Leu Lys Ser Gly Tyr Lys Asp Trp Ile Leu Trp Ile Ser Phe 
    530                 535                 540                 


Ala Ile Ser Ser Leu Val Leu Leu Val Ala Leu Leu Gly Phe Ile Met 
545                 550                 555                 560 


Trp Ala Cys Gln Lys Gly Asn Ile Arg Cys Asn Ile Cys Ile 
                565                 570                 


<210>  94
<211>  51
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer H3Kansas(S228N).c

<400>  94
ggatatccct aacagaataa gcatctattg gacaatagta aaaccgggag a                51


<210>  95
<211>  51
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer H3Kansas(S228N).r

<400>  95
cttattctgt tagggatatc ccttattctg ggtctagatc cgatattcgg g                51


<210>  96
<211>  1725
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PDI-H3 A/Kansas/14/2017 Y98F, S228Q

<400>  96
atggcgaaaa acgttgcgat tttcggctta ttgttttctc ttcttgtgtt ggttccttct       60

cagatcttcg cgcaaaaaat ccctggaaat gacaatagca cggcaacgct gtgccttggg      120

caccatgcag taccaaacgg aacgatagtg aaaacaatca cgaatgaccg aattgaagtt      180

actaatgcta ctgagctggt tcagaattcc tcaataggtg aaatatgcga cagtcctcat      240

cagatccttg atggagaaaa ctgcacacta atagatgctc tattgggaga ccctcagtgt      300

gatggctttc aaaataagaa atgggacctt tttgttgaac gaaacaaagc ctacagcaac      360

tgtttccctt atgatgtgcc ggattatgcc tcccttaggt cactagttgc ctcatccggc      420

acactggagt ttaacaatga aagcttcaat tgggctggag tcactcaaaa cggaacaagt      480

tcttcttgca taaggggatc taagagtagt ttctttagta gattaaattg gttgacccac      540

ttaaactcca aatacccagc attaaacgtg actatgccaa acaatgaaca atttgacaaa      600

ttgtacattt ggggggttca ccacccgggt acggacaagg accaaatcag cctgtatgca      660

caatcatcag gaagaatcac agtatctacc aaaagaagcc aacaagctgt aatcccgaat      720

atcggatcta gacccagaat aagggatatc cctcagagaa taagcatcta ttggacaata      780

gtaaaaccgg gagacatact tttgattaac agcacaggga atctaattgc tcctaggggt      840

tacttcaaaa tacgaagtgg gaaaagctca ataatgagat cagatgcacc cattggcaaa      900

tgcaagtctg aatgcatcac tccaaatgga agcattccca atgacaaacc attccaaaat      960

gtaaacagga tcacatacgg ggcctgtccc agatatgtta agcaaagcac tctgaaattg     1020

gcaacaggaa tgcgaaatgt accagagaga caaactagag gcatatttgg cgcaatagcg     1080

ggtttcatag aaaatggttg ggagggaatg gtggatggtt ggtacggctt caggcatcaa     1140

aattctgagg gaagaggaca agcagcagat ctcaaaagca ctcaagcagc aatcgatcaa     1200

atcaatggga agctggctcg agttatcggg aaaaccaacg agaaattcca tcagattgaa     1260

aaagaattct cagaagtaga agggagaatt caggaccttg agaaatatgt tgaggacaca     1320

aaaatagatc tctggtcata caacgcggag cttcttgttg ccctggagaa ccaacataca     1380

attgatctaa ctgactcaga aatgaacaaa ctgtttgaaa aaacaaagaa gcaactgagg     1440

gaaaatgctg aggatatggg caatggttgt ttcaaaatat accacaaatg tgacaatgcc     1500

tgcatgggat caatcagaaa tggaacttat gaccacaacg tatacaggga tgaagcatta     1560

aacaaccggt tccagatcaa gggagttgag ctgaagtcag ggtacaaaga ttggatccta     1620

tggatttcct ttgccatatc atcccttgta ctgttagttg ctttgttggg gttcatcatg     1680

tgggcctgcc aaaagggcaa cattaggtgc aacatttgca tttga                     1725


<210>  97
<211>  574
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  PDI-H3 A/Kansas/14/2017 Y98F, S228Q

<400>  97

Met Ala Lys Asn Val Ala Ile Phe Gly Leu Leu Phe Ser Leu Leu Val 
1               5                   10                  15      


Leu Val Pro Ser Gln Ile Phe Ala Gln Lys Ile Pro Gly Asn Asp Asn 
            20                  25                  30          


Ser Thr Ala Thr Leu Cys Leu Gly His His Ala Val Pro Asn Gly Thr 
        35                  40                  45              


Ile Val Lys Thr Ile Thr Asn Asp Arg Ile Glu Val Thr Asn Ala Thr 
    50                  55                  60                  


Glu Leu Val Gln Asn Ser Ser Ile Gly Glu Ile Cys Asp Ser Pro His 
65                  70                  75                  80  


Gln Ile Leu Asp Gly Glu Asn Cys Thr Leu Ile Asp Ala Leu Leu Gly 
                85                  90                  95      


Asp Pro Gln Cys Asp Gly Phe Gln Asn Lys Lys Trp Asp Leu Phe Val 
            100                 105                 110         


Glu Arg Asn Lys Ala Tyr Ser Asn Cys Phe Pro Tyr Asp Val Pro Asp 
        115                 120                 125             


Tyr Ala Ser Leu Arg Ser Leu Val Ala Ser Ser Gly Thr Leu Glu Phe 
    130                 135                 140                 


Asn Asn Glu Ser Phe Asn Trp Ala Gly Val Thr Gln Asn Gly Thr Ser 
145                 150                 155                 160 


Ser Ser Cys Ile Arg Gly Ser Lys Ser Ser Phe Phe Ser Arg Leu Asn 
                165                 170                 175     


Trp Leu Thr His Leu Asn Ser Lys Tyr Pro Ala Leu Asn Val Thr Met 
            180                 185                 190         


Pro Asn Asn Glu Gln Phe Asp Lys Leu Tyr Ile Trp Gly Val His His 
        195                 200                 205             


Pro Gly Thr Asp Lys Asp Gln Ile Ser Leu Tyr Ala Gln Ser Ser Gly 
    210                 215                 220                 


Arg Ile Thr Val Ser Thr Lys Arg Ser Gln Gln Ala Val Ile Pro Asn 
225                 230                 235                 240 


Ile Gly Ser Arg Pro Arg Ile Arg Asp Ile Pro Gln Arg Ile Ser Ile 
                245                 250                 255     


Tyr Trp Thr Ile Val Lys Pro Gly Asp Ile Leu Leu Ile Asn Ser Thr 
            260                 265                 270         


Gly Asn Leu Ile Ala Pro Arg Gly Tyr Phe Lys Ile Arg Ser Gly Lys 
        275                 280                 285             


Ser Ser Ile Met Arg Ser Asp Ala Pro Ile Gly Lys Cys Lys Ser Glu 
    290                 295                 300                 


Cys Ile Thr Pro Asn Gly Ser Ile Pro Asn Asp Lys Pro Phe Gln Asn 
305                 310                 315                 320 


Val Asn Arg Ile Thr Tyr Gly Ala Cys Pro Arg Tyr Val Lys Gln Ser 
                325                 330                 335     


Thr Leu Lys Leu Ala Thr Gly Met Arg Asn Val Pro Glu Arg Gln Thr 
            340                 345                 350         


Arg Gly Ile Phe Gly Ala Ile Ala Gly Phe Ile Glu Asn Gly Trp Glu 
        355                 360                 365             


Gly Met Val Asp Gly Trp Tyr Gly Phe Arg His Gln Asn Ser Glu Gly 
    370                 375                 380                 


Arg Gly Gln Ala Ala Asp Leu Lys Ser Thr Gln Ala Ala Ile Asp Gln 
385                 390                 395                 400 


Ile Asn Gly Lys Leu Ala Arg Val Ile Gly Lys Thr Asn Glu Lys Phe 
                405                 410                 415     


His Gln Ile Glu Lys Glu Phe Ser Glu Val Glu Gly Arg Ile Gln Asp 
            420                 425                 430         


Leu Glu Lys Tyr Val Glu Asp Thr Lys Ile Asp Leu Trp Ser Tyr Asn 
        435                 440                 445             


Ala Glu Leu Leu Val Ala Leu Glu Asn Gln His Thr Ile Asp Leu Thr 
    450                 455                 460                 


Asp Ser Glu Met Asn Lys Leu Phe Glu Lys Thr Lys Lys Gln Leu Arg 
465                 470                 475                 480 


Glu Asn Ala Glu Asp Met Gly Asn Gly Cys Phe Lys Ile Tyr His Lys 
                485                 490                 495     


Cys Asp Asn Ala Cys Met Gly Ser Ile Arg Asn Gly Thr Tyr Asp His 
            500                 505                 510         


Asn Val Tyr Arg Asp Glu Ala Leu Asn Asn Arg Phe Gln Ile Lys Gly 
        515                 520                 525             


Val Glu Leu Lys Ser Gly Tyr Lys Asp Trp Ile Leu Trp Ile Ser Phe 
    530                 535                 540                 


Ala Ile Ser Ser Leu Val Leu Leu Val Ala Leu Leu Gly Phe Ile Met 
545                 550                 555                 560 


Trp Ala Cys Gln Lys Gly Asn Ile Arg Cys Asn Ile Cys Ile 
                565                 570                 


<210>  98
<211>  55
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer H3Kansas(S228Q).c

<400>  98
ggatatccct cagagaataa gcatctattg gacaatagta aaaccgggag acata            55


<210>  99
<211>  51
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer H3Kansas(S228Q).r

<400>  99
cttattctct gagggatatc ccttattctg ggtctagatc cgatattcgg g                51


<210>  100
<211>  1722
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PDI-H1 A/Idaho/7/18

<400>  100
atggcgaaaa acgttgcgat tttcggctta ttgttttctc ttcttgtgtt ggttccttct       60

cagatcttcg cggacacatt atgtataggt tatcatgcga acaattcaac agacactgta      120

gacacagtac tagaaaagaa tgtaacagta acacactctg ttaaccttct ggaagacaag      180

cataacggaa aactatgcaa actaagaggg gtagccccat tgcatttggg taaatgtaac      240

attgctggct ggatcctggg aaatccagag tgtgaatcac tctccacagc aagatcatgg      300

tcctacattg tggaaacatc taattcagac aatggaacgt gttacccagg agatttcatc      360

aattatgagg agctaagaga gcaattgagc tcagtgtcat catttgaaag gtttgagata      420

ttccccaaga caagttcatg gcccaatcat gactcgaaca aaggtgtaac ggcagcatgt      480

cctcacgctg gagcaaaaag cttctacaaa aacttgatat ggctagttaa aaaaggaaat      540

tcatacccaa agcttaacca aacctacatt aatgataaag ggaaagaagt cctcgtgctg      600

tggggcattc accatccacc cactactgct gaccaacaaa gtctctatca gaatgcagat      660

gcatatgttt ttgtggggac atcaagatac agcaagaagt tcaagccgga aatagcaaca      720

agacccaaag tgagggatca agaagggaga atgaactatt actggacact agtagagccg      780

ggagacaaaa taacattcga agcaactgga aatctagtgg taccgagata tgcattcaca      840

atggaaagaa atgctggatc tggtattatc atttcagata caccagtcca cgattgcaat      900

acaacttgtc agacacccga gggtgctata aacaccagcc tcccatttca gaatgtgcat      960

ccgatcacaa ttggaaaatg tccaaagtat gtaaaaagca caaaattgag actggccaca     1020

ggattgagga atgttccgtc tattcaatct agaggcctat tcggggccat tgccggcttc     1080

attgaagggg ggtggacagg gatggtagat ggatggtacg gttatcacca tcaaaatgag     1140

caggggtcag gatatgcagc cgacctgaag agcacacaaa atgccattga caagattact     1200

aacaaagtaa attctgttat tgaaaagatg aatacacagg acacagcagt gggtaaagag     1260

ttcaaccacc tggaaaaaag aatagagaat ctaaataaaa aagttgatga tggtttcctg     1320

gacatttgga cttacaatgc cgaactgttg gttctaatgg aaaatgaaag aactttggac     1380

tatcacgatt caaatgtgaa gaacttgtat gaaaaagtaa gaaaccagtt aaaaaacaat     1440

gccaaggaaa ttggaaacgg ctgctttgaa ttttaccaca aatgcgataa cacgtgcatg     1500

gaaagtgtca aaaatgggac ttatgactac ccaaaatact cagaggaagc aaaattaaac     1560

agagaaaaaa tagatggggt aaagctggaa tcaacaagga tttaccagat tttggcgatc     1620

tattcaactg tcgccagttc attggtactg gtagtctccc tgggggcaat cagcttctgg     1680

atgtgctcta atgggtctct acagtgtaga atatgtattt aa                        1722


<210>  101
<211>  573
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  PDI- H1 A/Idaho/7/18

<400>  101

Met Ala Lys Asn Val Ala Ile Phe Gly Leu Leu Phe Ser Leu Leu Val 
1               5                   10                  15      


Leu Val Pro Ser Gln Ile Phe Ala Asp Thr Leu Cys Ile Gly Tyr His 
            20                  25                  30          


Ala Asn Asn Ser Thr Asp Thr Val Asp Thr Val Leu Glu Lys Asn Val 
        35                  40                  45              


Thr Val Thr His Ser Val Asn Leu Leu Glu Asp Lys His Asn Gly Lys 
    50                  55                  60                  


Leu Cys Lys Leu Arg Gly Val Ala Pro Leu His Leu Gly Lys Cys Asn 
65                  70                  75                  80  


Ile Ala Gly Trp Ile Leu Gly Asn Pro Glu Cys Glu Ser Leu Ser Thr 
                85                  90                  95      


Ala Arg Ser Trp Ser Tyr Ile Val Glu Thr Ser Asn Ser Asp Asn Gly 
            100                 105                 110         


Thr Cys Tyr Pro Gly Asp Phe Ile Asn Tyr Glu Glu Leu Arg Glu Gln 
        115                 120                 125             


Leu Ser Ser Val Ser Ser Phe Glu Arg Phe Glu Ile Phe Pro Lys Thr 
    130                 135                 140                 


Ser Ser Trp Pro Asn His Asp Ser Asn Lys Gly Val Thr Ala Ala Cys 
145                 150                 155                 160 


Pro His Ala Gly Ala Lys Ser Phe Tyr Lys Asn Leu Ile Trp Leu Val 
                165                 170                 175     


Lys Lys Gly Asn Ser Tyr Pro Lys Leu Asn Gln Thr Tyr Ile Asn Asp 
            180                 185                 190         


Lys Gly Lys Glu Val Leu Val Leu Trp Gly Ile His His Pro Pro Thr 
        195                 200                 205             


Thr Ala Asp Gln Gln Ser Leu Tyr Gln Asn Ala Asp Ala Tyr Val Phe 
    210                 215                 220                 


Val Gly Thr Ser Arg Tyr Ser Lys Lys Phe Lys Pro Glu Ile Ala Thr 
225                 230                 235                 240 


Arg Pro Lys Val Arg Asp Gln Glu Gly Arg Met Asn Tyr Tyr Trp Thr 
                245                 250                 255     


Leu Val Glu Pro Gly Asp Lys Ile Thr Phe Glu Ala Thr Gly Asn Leu 
            260                 265                 270         


Val Val Pro Arg Tyr Ala Phe Thr Met Glu Arg Asn Ala Gly Ser Gly 
        275                 280                 285             


Ile Ile Ile Ser Asp Thr Pro Val His Asp Cys Asn Thr Thr Cys Gln 
    290                 295                 300                 


Thr Pro Glu Gly Ala Ile Asn Thr Ser Leu Pro Phe Gln Asn Val His 
305                 310                 315                 320 


Pro Ile Thr Ile Gly Lys Cys Pro Lys Tyr Val Lys Ser Thr Lys Leu 
                325                 330                 335     


Arg Leu Ala Thr Gly Leu Arg Asn Val Pro Ser Ile Gln Ser Arg Gly 
            340                 345                 350         


Leu Phe Gly Ala Ile Ala Gly Phe Ile Glu Gly Gly Trp Thr Gly Met 
        355                 360                 365             


Val Asp Gly Trp Tyr Gly Tyr His His Gln Asn Glu Gln Gly Ser Gly 
    370                 375                 380                 


Tyr Ala Ala Asp Leu Lys Ser Thr Gln Asn Ala Ile Asp Lys Ile Thr 
385                 390                 395                 400 


Asn Lys Val Asn Ser Val Ile Glu Lys Met Asn Thr Gln Asp Thr Ala 
                405                 410                 415     


Val Gly Lys Glu Phe Asn His Leu Glu Lys Arg Ile Glu Asn Leu Asn 
            420                 425                 430         


Lys Lys Val Asp Asp Gly Phe Leu Asp Ile Trp Thr Tyr Asn Ala Glu 
        435                 440                 445             


Leu Leu Val Leu Met Glu Asn Glu Arg Thr Leu Asp Tyr His Asp Ser 
    450                 455                 460                 


Asn Val Lys Asn Leu Tyr Glu Lys Val Arg Asn Gln Leu Lys Asn Asn 
465                 470                 475                 480 


Ala Lys Glu Ile Gly Asn Gly Cys Phe Glu Phe Tyr His Lys Cys Asp 
                485                 490                 495     


Asn Thr Cys Met Glu Ser Val Lys Asn Gly Thr Tyr Asp Tyr Pro Lys 
            500                 505                 510         


Tyr Ser Glu Glu Ala Lys Leu Asn Arg Glu Lys Ile Asp Gly Val Lys 
        515                 520                 525             


Leu Glu Ser Thr Arg Ile Tyr Gln Ile Leu Ala Ile Tyr Ser Thr Val 
    530                 535                 540                 


Ala Ser Ser Leu Val Leu Val Val Ser Leu Gly Ala Ile Ser Phe Trp 
545                 550                 555                 560 


Met Cys Ser Asn Gly Ser Leu Gln Cys Arg Ile Cys Ile 
                565                 570             


<210>  102
<211>  48
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer IF-H1_Cal-7-09.c

<400>  102
tctcagatct tcgcggacac attatgtata ggttatcatg cgaacaat                    48


<210>  103
<211>  46
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer IF-H1cTMCT.s1-4r

<400>  103
actaaagaaa ataggccttt aaatacatat tctacactgt agagac                      46


<210>  104
<211>  1722
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PDI-H1 A/Idaho/7/18 Y91F

<400>  104
atggcgaaaa acgttgcgat tttcggctta ttgttttctc ttcttgtgtt ggttccttct       60

cagatcttcg cggacacatt atgtataggt tatcatgcga acaattcaac agacactgta      120

gacacagtac tagaaaagaa tgtaacagta acacactctg ttaaccttct ggaagacaag      180

cataacggaa aactatgcaa actaagaggg gtagccccat tgcatttggg taaatgtaac      240

attgctggct ggatcctggg aaatccagag tgtgaatcac tctccacagc aagatcatgg      300

tcctacattg tggaaacatc taattcagac aatggaacgt gtttcccagg agatttcatc      360

aattatgagg agctaagaga gcaattgagc tcagtgtcat catttgaaag gtttgagata      420

ttccccaaga caagttcatg gcccaatcat gactcgaaca aaggtgtaac ggcagcatgt      480

cctcacgctg gagcaaaaag cttctacaaa aacttgatat ggctagttaa aaaaggaaat      540

tcatacccaa agcttaacca aacctacatt aatgataaag ggaaagaagt cctcgtgctg      600

tggggcattc accatccacc cactactgct gaccaacaaa gtctctatca gaatgcagat      660

gcatatgttt ttgtggggac atcaagatac agcaagaagt tcaagccgga aatagcaaca      720

agacccaaag tgagggatca agaagggaga atgaactatt actggacact agtagagccg      780

ggagacaaaa taacattcga agcaactgga aatctagtgg taccgagata tgcattcaca      840

atggaaagaa atgctggatc tggtattatc atttcagata caccagtcca cgattgcaat      900

acaacttgtc agacacccga gggtgctata aacaccagcc tcccatttca gaatgtgcat      960

ccgatcacaa ttggaaaatg tccaaagtat gtaaaaagca caaaattgag actggccaca     1020

ggattgagga atgttccgtc tattcaatct agaggcctat tcggggccat tgccggcttc     1080

attgaagggg ggtggacagg gatggtagat ggatggtacg gttatcacca tcaaaatgag     1140

caggggtcag gatatgcagc cgacctgaag agcacacaaa atgccattga caagattact     1200

aacaaagtaa attctgttat tgaaaagatg aatacacagg acacagcagt gggtaaagag     1260

ttcaaccacc tggaaaaaag aatagagaat ctaaataaaa aagttgatga tggtttcctg     1320

gacatttgga cttacaatgc cgaactgttg gttctaatgg aaaatgaaag aactttggac     1380

tatcacgatt caaatgtgaa gaacttgtat gaaaaagtaa gaaaccagtt aaaaaacaat     1440

gccaaggaaa ttggaaacgg ctgctttgaa ttttaccaca aatgcgataa cacgtgcatg     1500

gaaagtgtca aaaatgggac ttatgactac ccaaaatact cagaggaagc aaaattaaac     1560

agagaaaaaa tagatggggt aaagctggaa tcaacaagga tttaccagat tttggcgatc     1620

tattcaactg tcgccagttc attggtactg gtagtctccc tgggggcaat cagcttctgg     1680

atgtgctcta atgggtctct acagtgtaga atatgtattt aa                        1722


<210>  105
<211>  573
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  PDI- H1 A/Idaho/7/18 Y91F

<400>  105

Met Ala Lys Asn Val Ala Ile Phe Gly Leu Leu Phe Ser Leu Leu Val 
1               5                   10                  15      


Leu Val Pro Ser Gln Ile Phe Ala Asp Thr Leu Cys Ile Gly Tyr His 
            20                  25                  30          


Ala Asn Asn Ser Thr Asp Thr Val Asp Thr Val Leu Glu Lys Asn Val 
        35                  40                  45              


Thr Val Thr His Ser Val Asn Leu Leu Glu Asp Lys His Asn Gly Lys 
    50                  55                  60                  


Leu Cys Lys Leu Arg Gly Val Ala Pro Leu His Leu Gly Lys Cys Asn 
65                  70                  75                  80  


Ile Ala Gly Trp Ile Leu Gly Asn Pro Glu Cys Glu Ser Leu Ser Thr 
                85                  90                  95      


Ala Arg Ser Trp Ser Tyr Ile Val Glu Thr Ser Asn Ser Asp Asn Gly 
            100                 105                 110         


Thr Cys Phe Pro Gly Asp Phe Ile Asn Tyr Glu Glu Leu Arg Glu Gln 
        115                 120                 125             


Leu Ser Ser Val Ser Ser Phe Glu Arg Phe Glu Ile Phe Pro Lys Thr 
    130                 135                 140                 


Ser Ser Trp Pro Asn His Asp Ser Asn Lys Gly Val Thr Ala Ala Cys 
145                 150                 155                 160 


Pro His Ala Gly Ala Lys Ser Phe Tyr Lys Asn Leu Ile Trp Leu Val 
                165                 170                 175     


Lys Lys Gly Asn Ser Tyr Pro Lys Leu Asn Gln Thr Tyr Ile Asn Asp 
            180                 185                 190         


Lys Gly Lys Glu Val Leu Val Leu Trp Gly Ile His His Pro Pro Thr 
        195                 200                 205             


Thr Ala Asp Gln Gln Ser Leu Tyr Gln Asn Ala Asp Ala Tyr Val Phe 
    210                 215                 220                 


Val Gly Thr Ser Arg Tyr Ser Lys Lys Phe Lys Pro Glu Ile Ala Thr 
225                 230                 235                 240 


Arg Pro Lys Val Arg Asp Gln Glu Gly Arg Met Asn Tyr Tyr Trp Thr 
                245                 250                 255     


Leu Val Glu Pro Gly Asp Lys Ile Thr Phe Glu Ala Thr Gly Asn Leu 
            260                 265                 270         


Val Val Pro Arg Tyr Ala Phe Thr Met Glu Arg Asn Ala Gly Ser Gly 
        275                 280                 285             


Ile Ile Ile Ser Asp Thr Pro Val His Asp Cys Asn Thr Thr Cys Gln 
    290                 295                 300                 


Thr Pro Glu Gly Ala Ile Asn Thr Ser Leu Pro Phe Gln Asn Val His 
305                 310                 315                 320 


Pro Ile Thr Ile Gly Lys Cys Pro Lys Tyr Val Lys Ser Thr Lys Leu 
                325                 330                 335     


Arg Leu Ala Thr Gly Leu Arg Asn Val Pro Ser Ile Gln Ser Arg Gly 
            340                 345                 350         


Leu Phe Gly Ala Ile Ala Gly Phe Ile Glu Gly Gly Trp Thr Gly Met 
        355                 360                 365             


Val Asp Gly Trp Tyr Gly Tyr His His Gln Asn Glu Gln Gly Ser Gly 
    370                 375                 380                 


Tyr Ala Ala Asp Leu Lys Ser Thr Gln Asn Ala Ile Asp Lys Ile Thr 
385                 390                 395                 400 


Asn Lys Val Asn Ser Val Ile Glu Lys Met Asn Thr Gln Asp Thr Ala 
                405                 410                 415     


Val Gly Lys Glu Phe Asn His Leu Glu Lys Arg Ile Glu Asn Leu Asn 
            420                 425                 430         


Lys Lys Val Asp Asp Gly Phe Leu Asp Ile Trp Thr Tyr Asn Ala Glu 
        435                 440                 445             


Leu Leu Val Leu Met Glu Asn Glu Arg Thr Leu Asp Tyr His Asp Ser 
    450                 455                 460                 


Asn Val Lys Asn Leu Tyr Glu Lys Val Arg Asn Gln Leu Lys Asn Asn 
465                 470                 475                 480 


Ala Lys Glu Ile Gly Asn Gly Cys Phe Glu Phe Tyr His Lys Cys Asp 
                485                 490                 495     


Asn Thr Cys Met Glu Ser Val Lys Asn Gly Thr Tyr Asp Tyr Pro Lys 
            500                 505                 510         


Tyr Ser Glu Glu Ala Lys Leu Asn Arg Glu Lys Ile Asp Gly Val Lys 
        515                 520                 525             


Leu Glu Ser Thr Arg Ile Tyr Gln Ile Leu Ala Ile Tyr Ser Thr Val 
    530                 535                 540                 


Ala Ser Ser Leu Val Leu Val Val Ser Leu Gly Ala Ile Ser Phe Trp 
545                 550                 555                 560 


Met Cys Ser Asn Gly Ser Leu Gln Cys Arg Ile Cys Ile 
                565                 570             


<210>  106
<211>  48
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  H1_Idaho(Y91F).c

<400>  106
acaatggaac gtgtttccca ggagatttca tcaattatga ggagctaa                    48


<210>  107
<211>  48
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  H1_Idaho(Y91F).r

<400>  107
tgatgaaatc tcctgggaaa cacgttccat tgtctgaatt agatgttt                    48


<210>  108
<211>  551
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  A/Egypt/NO4915/14 (H5N1)

<400>  108

Asp Gln Ile Cys Ile Gly Tyr His Ala Asn Asn Ser Thr Glu Gln Val 
1               5                   10                  15      


Asp Thr Ile Met Glu Lys Asn Val Thr Val Thr His Ala Gln Asp Ile 
            20                  25                  30          


Leu Glu Lys Thr His Asn Gly Lys Leu Cys Asn Leu Asp Gly Val Lys 
        35                  40                  45              


Pro Leu Ile Leu Arg Asp Cys Ser Val Ala Gly Trp Leu Leu Gly Asn 
    50                  55                  60                  


Pro Met Cys Asp Glu Phe Leu Asn Val Pro Glu Trp Ser Tyr Ile Val 
65                  70                  75                  80  


Glu Lys Ile Asn Pro Ala Asn Asp Leu Cys Tyr Pro Gly Asn Phe Asn 
                85                  90                  95      


Asp Tyr Glu Glu Leu Lys His Leu Leu Ser Arg Ile Asn His Phe Glu 
            100                 105                 110         


Lys Ile Gln Ile Ile Pro Lys Asp Ser Trp Ser Asp His Glu Ala Ser 
        115                 120                 125             


Gly Val Ser Ser Ala Cys Pro Tyr Gln Gly Arg Ser Ser Phe Phe Arg 
    130                 135                 140                 


Asn Val Val Trp Leu Thr Lys Lys Asn Asp Ala Tyr Pro Thr Ile Lys 
145                 150                 155                 160 


Lys Ser Tyr Asn Asn Thr Asn Gln Glu Asp Leu Leu Val Leu Trp Gly 
                165                 170                 175     


Ile His His Pro Asn Asp Ala Ala Glu Gln Thr Arg Leu Tyr Gln Asn 
            180                 185                 190         


Pro Thr Thr Tyr Ile Ser Val Gly Thr Ser Thr Leu Asn Gln Arg Leu 
        195                 200                 205             


Val Pro Lys Ile Ala Thr Arg Ser Lys Val Asn Gly Gln Ser Gly Arg 
    210                 215                 220                 


Met Glu Phe Phe Trp Thr Ile Leu Lys Ser Asn Asp Ala Ile Asn Phe 
225                 230                 235                 240 


Glu Ser Asn Gly Asn Phe Ile Ala Pro Glu Asn Ala Tyr Lys Ile Val 
                245                 250                 255     


Lys Lys Gly Asp Ser Thr Ile Met Lys Ser Glu Leu Glu Tyr Ser Asn 
            260                 265                 270         


Cys Asn Thr Lys Cys Gln Thr Pro Ile Gly Ala Ile Asn Ser Ser Met 
        275                 280                 285             


Pro Phe His Asn Ile His Pro Leu Thr Ile Gly Glu Cys Pro Lys Tyr 
    290                 295                 300                 


Val Lys Ser Asn Arg Leu Val Leu Ala Thr Gly Leu Arg Asn Ser Pro 
305                 310                 315                 320 


Gln Gly Glu Lys Arg Arg Lys Lys Arg Gly Leu Phe Gly Ala Ile Ala 
                325                 330                 335     


Gly Phe Ile Glu Gly Gly Trp Gln Gly Met Val Asp Gly Trp Tyr Gly 
            340                 345                 350         


Tyr His His Ser Asn Glu Gln Gly Ser Gly Tyr Ala Ala Asp Lys Glu 
        355                 360                 365             


Ser Thr Gln Arg Ala Ile Asp Gly Val Thr Asn Lys Val Asn Ser Ile 
    370                 375                 380                 


Ile Asp Lys Met Asn Thr Gln Phe Glu Ala Val Gly Arg Glu Phe Asn 
385                 390                 395                 400 


Asn Leu Glu Arg Arg Ile Glu Asn Leu Asn Lys Lys Met Glu Asp Gly 
                405                 410                 415     


Phe Leu Asp Val Trp Thr Tyr Asn Ala Glu Leu Leu Val Leu Met Glu 
            420                 425                 430         


Asn Glu Arg Thr Leu Asp Phe His Asp Ser Asn Val Lys Asn Leu Tyr 
        435                 440                 445             


Asp Lys Val Arg Leu Gln Leu Arg Asp Asn Ala Lys Glu Leu Gly Asn 
    450                 455                 460                 


Gly Cys Phe Glu Phe Tyr His Arg Cys Asp Asn Glu Cys Met Glu Ser 
465                 470                 475                 480 


Val Arg Asn Gly Thr Tyr Asp Tyr Pro Gln Tyr Ser Glu Glu Ala Arg 
                485                 490                 495     


Leu Lys Arg Glu Glu Ile Ser Gly Val Lys Leu Glu Ser Ile Gly Thr 
            500                 505                 510         


Tyr Gln Ile Leu Ser Ile Tyr Ser Thr Val Ala Ser Ser Leu Ala Leu 
        515                 520                 525             


Ala Ile Met Val Ala Gly Leu Ser Leu Trp Met Cys Ser Asn Gly Ser 
    530                 535                 540                 


Leu Gln Cys Arg Ile Cys Ile 
545                 550     


<210>  109
<211>  542
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  A/Hangzhou/1/13 (H7N9)

<400>  109

Asp Lys Ile Cys Leu Gly His His Ala Val Ser Asn Gly Thr Lys Val 
1               5                   10                  15      


Asn Thr Leu Thr Glu Arg Gly Val Glu Val Val Asn Ala Thr Glu Thr 
            20                  25                  30          


Val Glu Arg Thr Asn Ile Pro Arg Ile Cys Ser Lys Gly Lys Arg Thr 
        35                  40                  45              


Val Asp Leu Gly Gln Cys Gly Leu Leu Gly Thr Ile Thr Gly Pro Pro 
    50                  55                  60                  


Gln Cys Asp Gln Phe Leu Glu Phe Ser Ala Asp Leu Ile Ile Glu Arg 
65                  70                  75                  80  


Arg Glu Gly Ser Asp Val Cys Tyr Pro Gly Lys Phe Val Asn Glu Glu 
                85                  90                  95      


Ala Leu Arg Gln Ile Leu Arg Glu Ser Gly Gly Ile Asp Lys Glu Ala 
            100                 105                 110         


Met Gly Phe Thr Tyr Ser Gly Ile Arg Thr Asn Gly Ala Thr Ser Ala 
        115                 120                 125             


Cys Arg Arg Ser Gly Ser Ser Phe Tyr Ala Glu Met Lys Trp Leu Leu 
    130                 135                 140                 


Ser Asn Thr Asp Asn Ala Ala Phe Pro Gln Met Thr Lys Ser Tyr Lys 
145                 150                 155                 160 


Asn Thr Arg Lys Ser Pro Ala Leu Ile Val Trp Gly Ile His His Ser 
                165                 170                 175     


Val Ser Thr Ala Glu Gln Thr Lys Leu Tyr Gly Ser Gly Asn Lys Leu 
            180                 185                 190         


Val Thr Val Gly Ser Ser Asn Tyr Gln Gln Ser Phe Val Pro Ser Pro 
        195                 200                 205             


Gly Ala Arg Pro Gln Val Asn Gly Ile Ser Gly Arg Ile Asp Phe His 
    210                 215                 220                 


Trp Leu Met Leu Asn Pro Asn Asp Thr Val Thr Phe Ser Phe Asn Gly 
225                 230                 235                 240 


Ala Phe Ile Ala Pro Asp Arg Ala Ser Phe Leu Arg Gly Lys Ser Met 
                245                 250                 255     


Gly Ile Gln Ser Gly Val Gln Val Asp Ala Asn Cys Glu Gly Asp Cys 
            260                 265                 270         


Tyr His Ser Gly Gly Thr Ile Ile Ser Asn Leu Pro Phe Gln Asn Ile 
        275                 280                 285             


Asp Ser Arg Ala Val Gly Lys Cys Pro Arg Tyr Val Lys Gln Arg Ser 
    290                 295                 300                 


Leu Leu Leu Ala Thr Gly Met Lys Asn Val Pro Glu Ile Pro Lys Gly 
305                 310                 315                 320 


Arg Gly Leu Phe Gly Ala Ile Ala Gly Phe Ile Glu Asn Gly Trp Glu 
                325                 330                 335     


Gly Leu Ile Asp Gly Trp Tyr Gly Phe Arg His Gln Asn Ala Gln Gly 
            340                 345                 350         


Glu Gly Thr Ala Ala Asp Tyr Lys Ser Thr Gln Ser Ala Ile Asp Gln 
        355                 360                 365             


Ile Thr Gly Lys Leu Asn Arg Leu Ile Glu Lys Thr Asn Gln Gln Phe 
    370                 375                 380                 


Glu Leu Ile Asp Asn Glu Phe Asn Glu Val Glu Lys Gln Ile Gly Asn 
385                 390                 395                 400 


Val Ile Asn Trp Thr Arg Asp Ser Ile Thr Glu Val Trp Ser Tyr Asn 
                405                 410                 415     


Ala Glu Leu Leu Val Ala Met Glu Asn Gln His Thr Ile Asp Leu Ala 
            420                 425                 430         


Asp Ser Glu Met Asp Lys Leu Tyr Glu Arg Val Lys Arg Gln Leu Arg 
        435                 440                 445             


Glu Asn Ala Glu Glu Asp Gly Thr Gly Cys Phe Glu Ile Phe His Lys 
    450                 455                 460                 


Cys Asp Asp Asp Cys Met Ala Ser Ile Arg Asn Asn Thr Tyr Asp His 
465                 470                 475                 480 


Ser Lys Tyr Arg Glu Glu Ala Met Gln Asn Arg Ile Gln Ile Asp Pro 
                485                 490                 495     


Val Lys Leu Ser Ser Gly Tyr Lys Asp Val Ile Leu Trp Phe Ser Phe 
            500                 505                 510         


Gly Ala Ser Cys Phe Ile Leu Leu Ala Ile Val Met Gly Leu Val Phe 
        515                 520                 525             


Ile Cys Val Lys Asn Gly Asn Met Arg Cys Thr Ile Cys Ile 
    530                 535                 540         


<210>  110
<211>  29
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Consensus amino acid sequence for transmembrane domain


<220>
<221>  misc_feature
<222>  (3)..(3)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (15)..(15)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (17)..(18)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (23)..(23)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (25)..(25)
<223>  Xaa can be any naturally occurring amino acid

<400>  110

Ile Leu Xaa Ile Tyr Tyr Ser Thr Val Ala Ile Ser Ser Leu Xaa Leu 
1               5                   10                  15      


Xaa Xaa Met Leu Ala Gly Xaa Ser Xaa Trp Met Cys Ser 
            20                  25                  


<210>  111
<211>  1725
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PDI-H3 Kan-S136D DNA

<400>  111
atggcgaaaa acgttgcgat tttcggctta ttgttttctc ttcttgtgtt ggttccttct       60

cagatcttcg cgcaaaaaat ccctggaaat gacaatagca cggcaacgct gtgccttggg      120

caccatgcag taccaaacgg aacgatagtg aaaacaatca cgaatgaccg aattgaagtt      180

actaatgcta ctgagctggt tcagaattcc tcaataggtg aaatatgcga cagtcctcat      240

cagatccttg atggagaaaa ctgcacacta atagatgctc tattgggaga ccctcagtgt      300

gatggctttc aaaataagaa atgggacctt tttgttgaac gaaacaaagc ctacagcaac      360

tgttaccctt atgatgtgcc ggattatgcc tcccttaggt cactagttgc ctcatccggc      420

acactggagt ttaacaatga aagcttcaat tgggctggag tcactcaaaa cggaacagac      480

tcttcttgca taaggggatc taagagtagt ttctttagta gattaaattg gttgacccac      540

ttaaactcca aatacccagc attaaacgtg actatgccaa acaatgaaca atttgacaaa      600

ttgtacattt ggggggttca ccacccgggt acggacaagg accaaatcag cctgtatgca      660

caatcatcag gaagaatcac agtatctacc aaaagaagcc aacaagctgt aatcccgaat      720

atcggatcta gacccagaat aagggatatc cctagcagaa taagcatcta ttggacaata      780

gtaaaaccgg gagacatact tttgattaac agcacaggga atctaattgc tcctaggggt      840

tacttcaaaa tacgaagtgg gaaaagctca ataatgagat cagatgcacc cattggcaaa      900

tgcaagtctg aatgcatcac tccaaatgga agcattccca atgacaaacc attccaaaat      960

gtaaacagga tcacatacgg ggcctgtccc agatatgtta agcaaagcac tctgaaattg     1020

gcaacaggaa tgcgaaatgt accagagaga caaactagag gcatatttgg cgcaatagcg     1080

ggtttcatag aaaatggttg ggagggaatg gtggatggtt ggtacggctt caggcatcaa     1140

aattctgagg gaagaggaca agcagcagat ctcaaaagca ctcaagcagc aatcgatcaa     1200

atcaatggga agctggctcg agttatcggg aaaaccaacg agaaattcca tcagattgaa     1260

aaagaattct cagaagtaga agggagaatt caggaccttg agaaatatgt tgaggacaca     1320

aaaatagatc tctggtcata caacgcggag cttcttgttg ccctggagaa ccaacataca     1380

attgatctaa ctgactcaga aatgaacaaa ctgtttgaaa aaacaaagaa gcaactgagg     1440

gaaaatgctg aggatatggg caatggttgt ttcaaaatat accacaaatg tgacaatgcc     1500

tgcatgggat caatcagaaa tggaacttat gaccacaacg tatacaggga tgaagcatta     1560

aacaaccggt tccagatcaa gggagttgag ctgaagtcag ggtacaaaga ttggatccta     1620

tggatttcct ttgccatatc atcccttgta ctgttagttg ctttgttggg gttcatcatg     1680

tgggcctgcc aaaagggcaa cattaggtgc aacatttgca tttga                     1725


<210>  112
<211>  574
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  PDI-H3 Kan-S136D AA

<400>  112

Met Ala Lys Asn Val Ala Ile Phe Gly Leu Leu Phe Ser Leu Leu Val 
1               5                   10                  15      


Leu Val Pro Ser Gln Ile Phe Ala Gln Lys Ile Pro Gly Asn Asp Asn 
            20                  25                  30          


Ser Thr Ala Thr Leu Cys Leu Gly His His Ala Val Pro Asn Gly Thr 
        35                  40                  45              


Ile Val Lys Thr Ile Thr Asn Asp Arg Ile Glu Val Thr Asn Ala Thr 
    50                  55                  60                  


Glu Leu Val Gln Asn Ser Ser Ile Gly Glu Ile Cys Asp Ser Pro His 
65                  70                  75                  80  


Gln Ile Leu Asp Gly Glu Asn Cys Thr Leu Ile Asp Ala Leu Leu Gly 
                85                  90                  95      


Asp Pro Gln Cys Asp Gly Phe Gln Asn Lys Lys Trp Asp Leu Phe Val 
            100                 105                 110         


Glu Arg Asn Lys Ala Tyr Ser Asn Cys Tyr Pro Tyr Asp Val Pro Asp 
        115                 120                 125             


Tyr Ala Ser Leu Arg Ser Leu Val Ala Ser Ser Gly Thr Leu Glu Phe 
    130                 135                 140                 


Asn Asn Glu Ser Phe Asn Trp Ala Gly Val Thr Gln Asn Gly Thr Asp 
145                 150                 155                 160 


Ser Ser Cys Ile Arg Gly Ser Lys Ser Ser Phe Phe Ser Arg Leu Asn 
                165                 170                 175     


Trp Leu Thr His Leu Asn Ser Lys Tyr Pro Ala Leu Asn Val Thr Met 
            180                 185                 190         


Pro Asn Asn Glu Gln Phe Asp Lys Leu Tyr Ile Trp Gly Val His His 
        195                 200                 205             


Pro Gly Thr Asp Lys Asp Gln Ile Ser Leu Tyr Ala Gln Ser Ser Gly 
    210                 215                 220                 


Arg Ile Thr Val Ser Thr Lys Arg Ser Gln Gln Ala Val Ile Pro Asn 
225                 230                 235                 240 


Ile Gly Ser Arg Pro Arg Ile Arg Asp Ile Pro Ser Arg Ile Ser Ile 
                245                 250                 255     


Tyr Trp Thr Ile Val Lys Pro Gly Asp Ile Leu Leu Ile Asn Ser Thr 
            260                 265                 270         


Gly Asn Leu Ile Ala Pro Arg Gly Tyr Phe Lys Ile Arg Ser Gly Lys 
        275                 280                 285             


Ser Ser Ile Met Arg Ser Asp Ala Pro Ile Gly Lys Cys Lys Ser Glu 
    290                 295                 300                 


Cys Ile Thr Pro Asn Gly Ser Ile Pro Asn Asp Lys Pro Phe Gln Asn 
305                 310                 315                 320 


Val Asn Arg Ile Thr Tyr Gly Ala Cys Pro Arg Tyr Val Lys Gln Ser 
                325                 330                 335     


Thr Leu Lys Leu Ala Thr Gly Met Arg Asn Val Pro Glu Arg Gln Thr 
            340                 345                 350         


Arg Gly Ile Phe Gly Ala Ile Ala Gly Phe Ile Glu Asn Gly Trp Glu 
        355                 360                 365             


Gly Met Val Asp Gly Trp Tyr Gly Phe Arg His Gln Asn Ser Glu Gly 
    370                 375                 380                 


Arg Gly Gln Ala Ala Asp Leu Lys Ser Thr Gln Ala Ala Ile Asp Gln 
385                 390                 395                 400 


Ile Asn Gly Lys Leu Ala Arg Val Ile Gly Lys Thr Asn Glu Lys Phe 
                405                 410                 415     


His Gln Ile Glu Lys Glu Phe Ser Glu Val Glu Gly Arg Ile Gln Asp 
            420                 425                 430         


Leu Glu Lys Tyr Val Glu Asp Thr Lys Ile Asp Leu Trp Ser Tyr Asn 
        435                 440                 445             


Ala Glu Leu Leu Val Ala Leu Glu Asn Gln His Thr Ile Asp Leu Thr 
    450                 455                 460                 


Asp Ser Glu Met Asn Lys Leu Phe Glu Lys Thr Lys Lys Gln Leu Arg 
465                 470                 475                 480 


Glu Asn Ala Glu Asp Met Gly Asn Gly Cys Phe Lys Ile Tyr His Lys 
                485                 490                 495     


Cys Asp Asn Ala Cys Met Gly Ser Ile Arg Asn Gly Thr Tyr Asp His 
            500                 505                 510         


Asn Val Tyr Arg Asp Glu Ala Leu Asn Asn Arg Phe Gln Ile Lys Gly 
        515                 520                 525             


Val Glu Leu Lys Ser Gly Tyr Lys Asp Trp Ile Leu Trp Ile Ser Phe 
    530                 535                 540                 


Ala Ile Ser Ser Leu Val Leu Leu Val Ala Leu Leu Gly Phe Ile Met 
545                 550                 555                 560 


Trp Ala Cys Gln Lys Gly Asn Ile Arg Cys Asn Ile Cys Ile 
                565                 570                 


<210>  113
<211>  1725
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PDI-H3 Kan-S136N DNA

<400>  113
atggcgaaaa acgttgcgat tttcggctta ttgttttctc ttcttgtgtt ggttccttct       60

cagatcttcg cgcaaaaaat ccctggaaat gacaatagca cggcaacgct gtgccttggg      120

caccatgcag taccaaacgg aacgatagtg aaaacaatca cgaatgaccg aattgaagtt      180

actaatgcta ctgagctggt tcagaattcc tcaataggtg aaatatgcga cagtcctcat      240

cagatccttg atggagaaaa ctgcacacta atagatgctc tattgggaga ccctcagtgt      300

gatggctttc aaaataagaa atgggacctt tttgttgaac gaaacaaagc ctacagcaac      360

tgttaccctt atgatgtgcc ggattatgcc tcccttaggt cactagttgc ctcatccggc      420

acactggagt ttaacaatga aagcttcaat tgggctggag tcactcaaaa cggaacaaac      480

tcttcttgca taaggggatc taagagtagt ttctttagta gattaaattg gttgacccac      540

ttaaactcca aatacccagc attaaacgtg actatgccaa acaatgaaca atttgacaaa      600

ttgtacattt ggggggttca ccacccgggt acggacaagg accaaatcag cctgtatgca      660

caatcatcag gaagaatcac agtatctacc aaaagaagcc aacaagctgt aatcccgaat      720

atcggatcta gacccagaat aagggatatc cctagcagaa taagcatcta ttggacaata      780

gtaaaaccgg gagacatact tttgattaac agcacaggga atctaattgc tcctaggggt      840

tacttcaaaa tacgaagtgg gaaaagctca ataatgagat cagatgcacc cattggcaaa      900

tgcaagtctg aatgcatcac tccaaatgga agcattccca atgacaaacc attccaaaat      960

gtaaacagga tcacatacgg ggcctgtccc agatatgtta agcaaagcac tctgaaattg     1020

gcaacaggaa tgcgaaatgt accagagaga caaactagag gcatatttgg cgcaatagcg     1080

ggtttcatag aaaatggttg ggagggaatg gtggatggtt ggtacggctt caggcatcaa     1140

aattctgagg gaagaggaca agcagcagat ctcaaaagca ctcaagcagc aatcgatcaa     1200

atcaatggga agctggctcg agttatcggg aaaaccaacg agaaattcca tcagattgaa     1260

aaagaattct cagaagtaga agggagaatt caggaccttg agaaatatgt tgaggacaca     1320

aaaatagatc tctggtcata caacgcggag cttcttgttg ccctggagaa ccaacataca     1380

attgatctaa ctgactcaga aatgaacaaa ctgtttgaaa aaacaaagaa gcaactgagg     1440

gaaaatgctg aggatatggg caatggttgt ttcaaaatat accacaaatg tgacaatgcc     1500

tgcatgggat caatcagaaa tggaacttat gaccacaacg tatacaggga tgaagcatta     1560

aacaaccggt tccagatcaa gggagttgag ctgaagtcag ggtacaaaga ttggatccta     1620

tggatttcct ttgccatatc atcccttgta ctgttagttg ctttgttggg gttcatcatg     1680

tgggcctgcc aaaagggcaa cattaggtgc aacatttgca tttga                     1725


<210>  114
<211>  574
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  PDI-H3 Kan-S136N AA

<400>  114

Met Ala Lys Asn Val Ala Ile Phe Gly Leu Leu Phe Ser Leu Leu Val 
1               5                   10                  15      


Leu Val Pro Ser Gln Ile Phe Ala Gln Lys Ile Pro Gly Asn Asp Asn 
            20                  25                  30          


Ser Thr Ala Thr Leu Cys Leu Gly His His Ala Val Pro Asn Gly Thr 
        35                  40                  45              


Ile Val Lys Thr Ile Thr Asn Asp Arg Ile Glu Val Thr Asn Ala Thr 
    50                  55                  60                  


Glu Leu Val Gln Asn Ser Ser Ile Gly Glu Ile Cys Asp Ser Pro His 
65                  70                  75                  80  


Gln Ile Leu Asp Gly Glu Asn Cys Thr Leu Ile Asp Ala Leu Leu Gly 
                85                  90                  95      


Asp Pro Gln Cys Asp Gly Phe Gln Asn Lys Lys Trp Asp Leu Phe Val 
            100                 105                 110         


Glu Arg Asn Lys Ala Tyr Ser Asn Cys Tyr Pro Tyr Asp Val Pro Asp 
        115                 120                 125             


Tyr Ala Ser Leu Arg Ser Leu Val Ala Ser Ser Gly Thr Leu Glu Phe 
    130                 135                 140                 


Asn Asn Glu Ser Phe Asn Trp Ala Gly Val Thr Gln Asn Gly Thr Asn 
145                 150                 155                 160 


Ser Ser Cys Ile Arg Gly Ser Lys Ser Ser Phe Phe Ser Arg Leu Asn 
                165                 170                 175     


Trp Leu Thr His Leu Asn Ser Lys Tyr Pro Ala Leu Asn Val Thr Met 
            180                 185                 190         


Pro Asn Asn Glu Gln Phe Asp Lys Leu Tyr Ile Trp Gly Val His His 
        195                 200                 205             


Pro Gly Thr Asp Lys Asp Gln Ile Ser Leu Tyr Ala Gln Ser Ser Gly 
    210                 215                 220                 


Arg Ile Thr Val Ser Thr Lys Arg Ser Gln Gln Ala Val Ile Pro Asn 
225                 230                 235                 240 


Ile Gly Ser Arg Pro Arg Ile Arg Asp Ile Pro Ser Arg Ile Ser Ile 
                245                 250                 255     


Tyr Trp Thr Ile Val Lys Pro Gly Asp Ile Leu Leu Ile Asn Ser Thr 
            260                 265                 270         


Gly Asn Leu Ile Ala Pro Arg Gly Tyr Phe Lys Ile Arg Ser Gly Lys 
        275                 280                 285             


Ser Ser Ile Met Arg Ser Asp Ala Pro Ile Gly Lys Cys Lys Ser Glu 
    290                 295                 300                 


Cys Ile Thr Pro Asn Gly Ser Ile Pro Asn Asp Lys Pro Phe Gln Asn 
305                 310                 315                 320 


Val Asn Arg Ile Thr Tyr Gly Ala Cys Pro Arg Tyr Val Lys Gln Ser 
                325                 330                 335     


Thr Leu Lys Leu Ala Thr Gly Met Arg Asn Val Pro Glu Arg Gln Thr 
            340                 345                 350         


Arg Gly Ile Phe Gly Ala Ile Ala Gly Phe Ile Glu Asn Gly Trp Glu 
        355                 360                 365             


Gly Met Val Asp Gly Trp Tyr Gly Phe Arg His Gln Asn Ser Glu Gly 
    370                 375                 380                 


Arg Gly Gln Ala Ala Asp Leu Lys Ser Thr Gln Ala Ala Ile Asp Gln 
385                 390                 395                 400 


Ile Asn Gly Lys Leu Ala Arg Val Ile Gly Lys Thr Asn Glu Lys Phe 
                405                 410                 415     


His Gln Ile Glu Lys Glu Phe Ser Glu Val Glu Gly Arg Ile Gln Asp 
            420                 425                 430         


Leu Glu Lys Tyr Val Glu Asp Thr Lys Ile Asp Leu Trp Ser Tyr Asn 
        435                 440                 445             


Ala Glu Leu Leu Val Ala Leu Glu Asn Gln His Thr Ile Asp Leu Thr 
    450                 455                 460                 


Asp Ser Glu Met Asn Lys Leu Phe Glu Lys Thr Lys Lys Gln Leu Arg 
465                 470                 475                 480 


Glu Asn Ala Glu Asp Met Gly Asn Gly Cys Phe Lys Ile Tyr His Lys 
                485                 490                 495     


Cys Asp Asn Ala Cys Met Gly Ser Ile Arg Asn Gly Thr Tyr Asp His 
            500                 505                 510         


Asn Val Tyr Arg Asp Glu Ala Leu Asn Asn Arg Phe Gln Ile Lys Gly 
        515                 520                 525             


Val Glu Leu Lys Ser Gly Tyr Lys Asp Trp Ile Leu Trp Ile Ser Phe 
    530                 535                 540                 


Ala Ile Ser Ser Leu Val Leu Leu Val Ala Leu Leu Gly Phe Ile Met 
545                 550                 555                 560 


Trp Ala Cys Gln Lys Gly Asn Ile Arg Cys Asn Ile Cys Ile 
                565                 570                 


<210>  115
<211>  1725
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PDI-H3 Kan-D190K DNA

<400>  115
atggcgaaaa acgttgcgat tttcggctta ttgttttctc ttcttgtgtt ggttccttct       60

cagatcttcg cgcaaaaaat ccctggaaat gacaatagca cggcaacgct gtgccttggg      120

caccatgcag taccaaacgg aacgatagtg aaaacaatca cgaatgaccg aattgaagtt      180

actaatgcta ctgagctggt tcagaattcc tcaataggtg aaatatgcga cagtcctcat      240

cagatccttg atggagaaaa ctgcacacta atagatgctc tattgggaga ccctcagtgt      300

gatggctttc aaaataagaa atgggacctt tttgttgaac gaaacaaagc ctacagcaac      360

tgttaccctt atgatgtgcc ggattatgcc tcccttaggt cactagttgc ctcatccggc      420

acactggagt ttaacaatga aagcttcaat tgggctggag tcactcaaaa cggaacaagt      480

tcttcttgca taaggggatc taagagtagt ttctttagta gattaaattg gttgacccac      540

ttaaactcca aatacccagc attaaacgtg actatgccaa acaatgaaca atttgacaaa      600

ttgtacattt ggggggttca ccacccgggt acggacaaga agcaaatcag cctgtatgca      660

caatcatcag gaagaatcac agtatctacc aaaagaagcc aacaagctgt aatcccgaat      720

atcggatcta gacccagaat aagggatatc cctagcagaa taagcatcta ttggacaata      780

gtaaaaccgg gagacatact tttgattaac agcacaggga atctaattgc tcctaggggt      840

tacttcaaaa tacgaagtgg gaaaagctca ataatgagat cagatgcacc cattggcaaa      900

tgcaagtctg aatgcatcac tccaaatgga agcattccca atgacaaacc attccaaaat      960

gtaaacagga tcacatacgg ggcctgtccc agatatgtta agcaaagcac tctgaaattg     1020

gcaacaggaa tgcgaaatgt accagagaga caaactagag gcatatttgg cgcaatagcg     1080

ggtttcatag aaaatggttg ggagggaatg gtggatggtt ggtacggctt caggcatcaa     1140

aattctgagg gaagaggaca agcagcagat ctcaaaagca ctcaagcagc aatcgatcaa     1200

atcaatggga agctggctcg agttatcggg aaaaccaacg agaaattcca tcagattgaa     1260

aaagaattct cagaagtaga agggagaatt caggaccttg agaaatatgt tgaggacaca     1320

aaaatagatc tctggtcata caacgcggag cttcttgttg ccctggagaa ccaacataca     1380

attgatctaa ctgactcaga aatgaacaaa ctgtttgaaa aaacaaagaa gcaactgagg     1440

gaaaatgctg aggatatggg caatggttgt ttcaaaatat accacaaatg tgacaatgcc     1500

tgcatgggat caatcagaaa tggaacttat gaccacaacg tatacaggga tgaagcatta     1560

aacaaccggt tccagatcaa gggagttgag ctgaagtcag ggtacaaaga ttggatccta     1620

tggatttcct ttgccatatc atcccttgta ctgttagttg ctttgttggg gttcatcatg     1680

tgggcctgcc aaaagggcaa cattaggtgc aacatttgca tttga                     1725


<210>  116
<211>  574
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  PDI-H3 Kan-D190K AA

<400>  116

Met Ala Lys Asn Val Ala Ile Phe Gly Leu Leu Phe Ser Leu Leu Val 
1               5                   10                  15      


Leu Val Pro Ser Gln Ile Phe Ala Gln Lys Ile Pro Gly Asn Asp Asn 
            20                  25                  30          


Ser Thr Ala Thr Leu Cys Leu Gly His His Ala Val Pro Asn Gly Thr 
        35                  40                  45              


Ile Val Lys Thr Ile Thr Asn Asp Arg Ile Glu Val Thr Asn Ala Thr 
    50                  55                  60                  


Glu Leu Val Gln Asn Ser Ser Ile Gly Glu Ile Cys Asp Ser Pro His 
65                  70                  75                  80  


Gln Ile Leu Asp Gly Glu Asn Cys Thr Leu Ile Asp Ala Leu Leu Gly 
                85                  90                  95      


Asp Pro Gln Cys Asp Gly Phe Gln Asn Lys Lys Trp Asp Leu Phe Val 
            100                 105                 110         


Glu Arg Asn Lys Ala Tyr Ser Asn Cys Tyr Pro Tyr Asp Val Pro Asp 
        115                 120                 125             


Tyr Ala Ser Leu Arg Ser Leu Val Ala Ser Ser Gly Thr Leu Glu Phe 
    130                 135                 140                 


Asn Asn Glu Ser Phe Asn Trp Ala Gly Val Thr Gln Asn Gly Thr Ser 
145                 150                 155                 160 


Ser Ser Cys Ile Arg Gly Ser Lys Ser Ser Phe Phe Ser Arg Leu Asn 
                165                 170                 175     


Trp Leu Thr His Leu Asn Ser Lys Tyr Pro Ala Leu Asn Val Thr Met 
            180                 185                 190         


Pro Asn Asn Glu Gln Phe Asp Lys Leu Tyr Ile Trp Gly Val His His 
        195                 200                 205             


Pro Gly Thr Asp Lys Lys Gln Ile Ser Leu Tyr Ala Gln Ser Ser Gly 
    210                 215                 220                 


Arg Ile Thr Val Ser Thr Lys Arg Ser Gln Gln Ala Val Ile Pro Asn 
225                 230                 235                 240 


Ile Gly Ser Arg Pro Arg Ile Arg Asp Ile Pro Ser Arg Ile Ser Ile 
                245                 250                 255     


Tyr Trp Thr Ile Val Lys Pro Gly Asp Ile Leu Leu Ile Asn Ser Thr 
            260                 265                 270         


Gly Asn Leu Ile Ala Pro Arg Gly Tyr Phe Lys Ile Arg Ser Gly Lys 
        275                 280                 285             


Ser Ser Ile Met Arg Ser Asp Ala Pro Ile Gly Lys Cys Lys Ser Glu 
    290                 295                 300                 


Cys Ile Thr Pro Asn Gly Ser Ile Pro Asn Asp Lys Pro Phe Gln Asn 
305                 310                 315                 320 


Val Asn Arg Ile Thr Tyr Gly Ala Cys Pro Arg Tyr Val Lys Gln Ser 
                325                 330                 335     


Thr Leu Lys Leu Ala Thr Gly Met Arg Asn Val Pro Glu Arg Gln Thr 
            340                 345                 350         


Arg Gly Ile Phe Gly Ala Ile Ala Gly Phe Ile Glu Asn Gly Trp Glu 
        355                 360                 365             


Gly Met Val Asp Gly Trp Tyr Gly Phe Arg His Gln Asn Ser Glu Gly 
    370                 375                 380                 


Arg Gly Gln Ala Ala Asp Leu Lys Ser Thr Gln Ala Ala Ile Asp Gln 
385                 390                 395                 400 


Ile Asn Gly Lys Leu Ala Arg Val Ile Gly Lys Thr Asn Glu Lys Phe 
                405                 410                 415     


His Gln Ile Glu Lys Glu Phe Ser Glu Val Glu Gly Arg Ile Gln Asp 
            420                 425                 430         


Leu Glu Lys Tyr Val Glu Asp Thr Lys Ile Asp Leu Trp Ser Tyr Asn 
        435                 440                 445             


Ala Glu Leu Leu Val Ala Leu Glu Asn Gln His Thr Ile Asp Leu Thr 
    450                 455                 460                 


Asp Ser Glu Met Asn Lys Leu Phe Glu Lys Thr Lys Lys Gln Leu Arg 
465                 470                 475                 480 


Glu Asn Ala Glu Asp Met Gly Asn Gly Cys Phe Lys Ile Tyr His Lys 
                485                 490                 495     


Cys Asp Asn Ala Cys Met Gly Ser Ile Arg Asn Gly Thr Tyr Asp His 
            500                 505                 510         


Asn Val Tyr Arg Asp Glu Ala Leu Asn Asn Arg Phe Gln Ile Lys Gly 
        515                 520                 525             


Val Glu Leu Lys Ser Gly Tyr Lys Asp Trp Ile Leu Trp Ile Ser Phe 
    530                 535                 540                 


Ala Ile Ser Ser Leu Val Leu Leu Val Ala Leu Leu Gly Phe Ile Met 
545                 550                 555                 560 


Trp Ala Cys Gln Lys Gly Asn Ile Arg Cys Asn Ile Cys Ile 
                565                 570                 


<210>  117
<211>  1725
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PDI-H3 Kan-R222W DNA

<400>  117
atggcgaaaa acgttgcgat tttcggctta ttgttttctc ttcttgtgtt ggttccttct       60

cagatcttcg cgcaaaaaat ccctggaaat gacaatagca cggcaacgct gtgccttggg      120

caccatgcag taccaaacgg aacgatagtg aaaacaatca cgaatgaccg aattgaagtt      180

actaatgcta ctgagctggt tcagaattcc tcaataggtg aaatatgcga cagtcctcat      240

cagatccttg atggagaaaa ctgcacacta atagatgctc tattgggaga ccctcagtgt      300

gatggctttc aaaataagaa atgggacctt tttgttgaac gaaacaaagc ctacagcaac      360

tgttaccctt atgatgtgcc ggattatgcc tcccttaggt cactagttgc ctcatccggc      420

acactggagt ttaacaatga aagcttcaat tgggctggag tcactcaaaa cggaacaagt      480

tcttcttgca taaggggatc taagagtagt ttctttagta gattaaattg gttgacccac      540

ttaaactcca aatacccagc attaaacgtg actatgccaa acaatgaaca atttgacaaa      600

ttgtacattt ggggggttca ccacccgggt acggacaagg accaaatcag cctgtatgca      660

caatcatcag gaagaatcac agtatctacc aaaagaagcc aacaagctgt aatcccgaat      720

atcggatcta gaccctggat aagggatatc cctagcagaa taagcatcta ttggacaata      780

gtaaaaccgg gagacatact tttgattaac agcacaggga atctaattgc tcctaggggt      840

tacttcaaaa tacgaagtgg gaaaagctca ataatgagat cagatgcacc cattggcaaa      900

tgcaagtctg aatgcatcac tccaaatgga agcattccca atgacaaacc attccaaaat      960

gtaaacagga tcacatacgg ggcctgtccc agatatgtta agcaaagcac tctgaaattg     1020

gcaacaggaa tgcgaaatgt accagagaga caaactagag gcatatttgg cgcaatagcg     1080

ggtttcatag aaaatggttg ggagggaatg gtggatggtt ggtacggctt caggcatcaa     1140

aattctgagg gaagaggaca agcagcagat ctcaaaagca ctcaagcagc aatcgatcaa     1200

atcaatggga agctggctcg agttatcggg aaaaccaacg agaaattcca tcagattgaa     1260

aaagaattct cagaagtaga agggagaatt caggaccttg agaaatatgt tgaggacaca     1320

aaaatagatc tctggtcata caacgcggag cttcttgttg ccctggagaa ccaacataca     1380

attgatctaa ctgactcaga aatgaacaaa ctgtttgaaa aaacaaagaa gcaactgagg     1440

gaaaatgctg aggatatggg caatggttgt ttcaaaatat accacaaatg tgacaatgcc     1500

tgcatgggat caatcagaaa tggaacttat gaccacaacg tatacaggga tgaagcatta     1560

aacaaccggt tccagatcaa gggagttgag ctgaagtcag ggtacaaaga ttggatccta     1620

tggatttcct ttgccatatc atcccttgta ctgttagttg ctttgttggg gttcatcatg     1680

tgggcctgcc aaaagggcaa cattaggtgc aacatttgca tttga                     1725


<210>  118
<211>  574
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  PDI-H3 Kan-R222W AA

<400>  118

Met Ala Lys Asn Val Ala Ile Phe Gly Leu Leu Phe Ser Leu Leu Val 
1               5                   10                  15      


Leu Val Pro Ser Gln Ile Phe Ala Gln Lys Ile Pro Gly Asn Asp Asn 
            20                  25                  30          


Ser Thr Ala Thr Leu Cys Leu Gly His His Ala Val Pro Asn Gly Thr 
        35                  40                  45              


Ile Val Lys Thr Ile Thr Asn Asp Arg Ile Glu Val Thr Asn Ala Thr 
    50                  55                  60                  


Glu Leu Val Gln Asn Ser Ser Ile Gly Glu Ile Cys Asp Ser Pro His 
65                  70                  75                  80  


Gln Ile Leu Asp Gly Glu Asn Cys Thr Leu Ile Asp Ala Leu Leu Gly 
                85                  90                  95      


Asp Pro Gln Cys Asp Gly Phe Gln Asn Lys Lys Trp Asp Leu Phe Val 
            100                 105                 110         


Glu Arg Asn Lys Ala Tyr Ser Asn Cys Tyr Pro Tyr Asp Val Pro Asp 
        115                 120                 125             


Tyr Ala Ser Leu Arg Ser Leu Val Ala Ser Ser Gly Thr Leu Glu Phe 
    130                 135                 140                 


Asn Asn Glu Ser Phe Asn Trp Ala Gly Val Thr Gln Asn Gly Thr Ser 
145                 150                 155                 160 


Ser Ser Cys Ile Arg Gly Ser Lys Ser Ser Phe Phe Ser Arg Leu Asn 
                165                 170                 175     


Trp Leu Thr His Leu Asn Ser Lys Tyr Pro Ala Leu Asn Val Thr Met 
            180                 185                 190         


Pro Asn Asn Glu Gln Phe Asp Lys Leu Tyr Ile Trp Gly Val His His 
        195                 200                 205             


Pro Gly Thr Asp Lys Asp Gln Ile Ser Leu Tyr Ala Gln Ser Ser Gly 
    210                 215                 220                 


Arg Ile Thr Val Ser Thr Lys Arg Ser Gln Gln Ala Val Ile Pro Asn 
225                 230                 235                 240 


Ile Gly Ser Arg Pro Trp Ile Arg Asp Ile Pro Ser Arg Ile Ser Ile 
                245                 250                 255     


Tyr Trp Thr Ile Val Lys Pro Gly Asp Ile Leu Leu Ile Asn Ser Thr 
            260                 265                 270         


Gly Asn Leu Ile Ala Pro Arg Gly Tyr Phe Lys Ile Arg Ser Gly Lys 
        275                 280                 285             


Ser Ser Ile Met Arg Ser Asp Ala Pro Ile Gly Lys Cys Lys Ser Glu 
    290                 295                 300                 


Cys Ile Thr Pro Asn Gly Ser Ile Pro Asn Asp Lys Pro Phe Gln Asn 
305                 310                 315                 320 


Val Asn Arg Ile Thr Tyr Gly Ala Cys Pro Arg Tyr Val Lys Gln Ser 
                325                 330                 335     


Thr Leu Lys Leu Ala Thr Gly Met Arg Asn Val Pro Glu Arg Gln Thr 
            340                 345                 350         


Arg Gly Ile Phe Gly Ala Ile Ala Gly Phe Ile Glu Asn Gly Trp Glu 
        355                 360                 365             


Gly Met Val Asp Gly Trp Tyr Gly Phe Arg His Gln Asn Ser Glu Gly 
    370                 375                 380                 


Arg Gly Gln Ala Ala Asp Leu Lys Ser Thr Gln Ala Ala Ile Asp Gln 
385                 390                 395                 400 


Ile Asn Gly Lys Leu Ala Arg Val Ile Gly Lys Thr Asn Glu Lys Phe 
                405                 410                 415     


His Gln Ile Glu Lys Glu Phe Ser Glu Val Glu Gly Arg Ile Gln Asp 
            420                 425                 430         


Leu Glu Lys Tyr Val Glu Asp Thr Lys Ile Asp Leu Trp Ser Tyr Asn 
        435                 440                 445             


Ala Glu Leu Leu Val Ala Leu Glu Asn Gln His Thr Ile Asp Leu Thr 
    450                 455                 460                 


Asp Ser Glu Met Asn Lys Leu Phe Glu Lys Thr Lys Lys Gln Leu Arg 
465                 470                 475                 480 


Glu Asn Ala Glu Asp Met Gly Asn Gly Cys Phe Lys Ile Tyr His Lys 
                485                 490                 495     


Cys Asp Asn Ala Cys Met Gly Ser Ile Arg Asn Gly Thr Tyr Asp His 
            500                 505                 510         


Asn Val Tyr Arg Asp Glu Ala Leu Asn Asn Arg Phe Gln Ile Lys Gly 
        515                 520                 525             


Val Glu Leu Lys Ser Gly Tyr Lys Asp Trp Ile Leu Trp Ile Ser Phe 
    530                 535                 540                 


Ala Ile Ser Ser Leu Val Leu Leu Val Ala Leu Leu Gly Phe Ile Met 
545                 550                 555                 560 


Trp Ala Cys Gln Lys Gly Asn Ile Arg Cys Asn Ile Cys Ile 
                565                 570                 


<210>  119
<211>  1725
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PDI-H3 Kan-S228N DNA

<400>  119
atggcgaaaa acgttgcgat tttcggctta ttgttttctc ttcttgtgtt ggttccttct       60

cagatcttcg cgcaaaaaat ccctggaaat gacaatagca cggcaacgct gtgccttggg      120

caccatgcag taccaaacgg aacgatagtg aaaacaatca cgaatgaccg aattgaagtt      180

actaatgcta ctgagctggt tcagaattcc tcaataggtg aaatatgcga cagtcctcat      240

cagatccttg atggagaaaa ctgcacacta atagatgctc tattgggaga ccctcagtgt      300

gatggctttc aaaataagaa atgggacctt tttgttgaac gaaacaaagc ctacagcaac      360

tgttaccctt atgatgtgcc ggattatgcc tcccttaggt cactagttgc ctcatccggc      420

acactggagt ttaacaatga aagcttcaat tgggctggag tcactcaaaa cggaacaagt      480

tcttcttgca taaggggatc taagagtagt ttctttagta gattaaattg gttgacccac      540

ttaaactcca aatacccagc attaaacgtg actatgccaa acaatgaaca atttgacaaa      600

ttgtacattt ggggggttca ccacccgggt acggacaagg accaaatcag cctgtatgca      660

caatcatcag gaagaatcac agtatctacc aaaagaagcc aacaagctgt aatcccgaat      720

atcggatcta gacccagaat aagggatatc cctaacagaa taagcatcta ttggacaata      780

gtaaaaccgg gagacatact tttgattaac agcacaggga atctaattgc tcctaggggt      840

tacttcaaaa tacgaagtgg gaaaagctca ataatgagat cagatgcacc cattggcaaa      900

tgcaagtctg aatgcatcac tccaaatgga agcattccca atgacaaacc attccaaaat      960

gtaaacagga tcacatacgg ggcctgtccc agatatgtta agcaaagcac tctgaaattg     1020

gcaacaggaa tgcgaaatgt accagagaga caaactagag gcatatttgg cgcaatagcg     1080

ggtttcatag aaaatggttg ggagggaatg gtggatggtt ggtacggctt caggcatcaa     1140

aattctgagg gaagaggaca agcagcagat ctcaaaagca ctcaagcagc aatcgatcaa     1200

atcaatggga agctggctcg agttatcggg aaaaccaacg agaaattcca tcagattgaa     1260

aaagaattct cagaagtaga agggagaatt caggaccttg agaaatatgt tgaggacaca     1320

aaaatagatc tctggtcata caacgcggag cttcttgttg ccctggagaa ccaacataca     1380

attgatctaa ctgactcaga aatgaacaaa ctgtttgaaa aaacaaagaa gcaactgagg     1440

gaaaatgctg aggatatggg caatggttgt ttcaaaatat accacaaatg tgacaatgcc     1500

tgcatgggat caatcagaaa tggaacttat gaccacaacg tatacaggga tgaagcatta     1560

aacaaccggt tccagatcaa gggagttgag ctgaagtcag ggtacaaaga ttggatccta     1620

tggatttcct ttgccatatc atcccttgta ctgttagttg ctttgttggg gttcatcatg     1680

tgggcctgcc aaaagggcaa cattaggtgc aacatttgca tttga                     1725


<210>  120
<211>  574
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  PDI-H3 Kan-S228N AA

<400>  120

Met Ala Lys Asn Val Ala Ile Phe Gly Leu Leu Phe Ser Leu Leu Val 
1               5                   10                  15      


Leu Val Pro Ser Gln Ile Phe Ala Gln Lys Ile Pro Gly Asn Asp Asn 
            20                  25                  30          


Ser Thr Ala Thr Leu Cys Leu Gly His His Ala Val Pro Asn Gly Thr 
        35                  40                  45              


Ile Val Lys Thr Ile Thr Asn Asp Arg Ile Glu Val Thr Asn Ala Thr 
    50                  55                  60                  


Glu Leu Val Gln Asn Ser Ser Ile Gly Glu Ile Cys Asp Ser Pro His 
65                  70                  75                  80  


Gln Ile Leu Asp Gly Glu Asn Cys Thr Leu Ile Asp Ala Leu Leu Gly 
                85                  90                  95      


Asp Pro Gln Cys Asp Gly Phe Gln Asn Lys Lys Trp Asp Leu Phe Val 
            100                 105                 110         


Glu Arg Asn Lys Ala Tyr Ser Asn Cys Tyr Pro Tyr Asp Val Pro Asp 
        115                 120                 125             


Tyr Ala Ser Leu Arg Ser Leu Val Ala Ser Ser Gly Thr Leu Glu Phe 
    130                 135                 140                 


Asn Asn Glu Ser Phe Asn Trp Ala Gly Val Thr Gln Asn Gly Thr Ser 
145                 150                 155                 160 


Ser Ser Cys Ile Arg Gly Ser Lys Ser Ser Phe Phe Ser Arg Leu Asn 
                165                 170                 175     


Trp Leu Thr His Leu Asn Ser Lys Tyr Pro Ala Leu Asn Val Thr Met 
            180                 185                 190         


Pro Asn Asn Glu Gln Phe Asp Lys Leu Tyr Ile Trp Gly Val His His 
        195                 200                 205             


Pro Gly Thr Asp Lys Asp Gln Ile Ser Leu Tyr Ala Gln Ser Ser Gly 
    210                 215                 220                 


Arg Ile Thr Val Ser Thr Lys Arg Ser Gln Gln Ala Val Ile Pro Asn 
225                 230                 235                 240 


Ile Gly Ser Arg Pro Arg Ile Arg Asp Ile Pro Asn Arg Ile Ser Ile 
                245                 250                 255     


Tyr Trp Thr Ile Val Lys Pro Gly Asp Ile Leu Leu Ile Asn Ser Thr 
            260                 265                 270         


Gly Asn Leu Ile Ala Pro Arg Gly Tyr Phe Lys Ile Arg Ser Gly Lys 
        275                 280                 285             


Ser Ser Ile Met Arg Ser Asp Ala Pro Ile Gly Lys Cys Lys Ser Glu 
    290                 295                 300                 


Cys Ile Thr Pro Asn Gly Ser Ile Pro Asn Asp Lys Pro Phe Gln Asn 
305                 310                 315                 320 


Val Asn Arg Ile Thr Tyr Gly Ala Cys Pro Arg Tyr Val Lys Gln Ser 
                325                 330                 335     


Thr Leu Lys Leu Ala Thr Gly Met Arg Asn Val Pro Glu Arg Gln Thr 
            340                 345                 350         


Arg Gly Ile Phe Gly Ala Ile Ala Gly Phe Ile Glu Asn Gly Trp Glu 
        355                 360                 365             


Gly Met Val Asp Gly Trp Tyr Gly Phe Arg His Gln Asn Ser Glu Gly 
    370                 375                 380                 


Arg Gly Gln Ala Ala Asp Leu Lys Ser Thr Gln Ala Ala Ile Asp Gln 
385                 390                 395                 400 


Ile Asn Gly Lys Leu Ala Arg Val Ile Gly Lys Thr Asn Glu Lys Phe 
                405                 410                 415     


His Gln Ile Glu Lys Glu Phe Ser Glu Val Glu Gly Arg Ile Gln Asp 
            420                 425                 430         


Leu Glu Lys Tyr Val Glu Asp Thr Lys Ile Asp Leu Trp Ser Tyr Asn 
        435                 440                 445             


Ala Glu Leu Leu Val Ala Leu Glu Asn Gln His Thr Ile Asp Leu Thr 
    450                 455                 460                 


Asp Ser Glu Met Asn Lys Leu Phe Glu Lys Thr Lys Lys Gln Leu Arg 
465                 470                 475                 480 


Glu Asn Ala Glu Asp Met Gly Asn Gly Cys Phe Lys Ile Tyr His Lys 
                485                 490                 495     


Cys Asp Asn Ala Cys Met Gly Ser Ile Arg Asn Gly Thr Tyr Asp His 
            500                 505                 510         


Asn Val Tyr Arg Asp Glu Ala Leu Asn Asn Arg Phe Gln Ile Lys Gly 
        515                 520                 525             


Val Glu Leu Lys Ser Gly Tyr Lys Asp Trp Ile Leu Trp Ile Ser Phe 
    530                 535                 540                 


Ala Ile Ser Ser Leu Val Leu Leu Val Ala Leu Leu Gly Phe Ile Met 
545                 550                 555                 560 


Trp Ala Cys Gln Lys Gly Asn Ile Arg Cys Asn Ile Cys Ile 
                565                 570                 


<210>  121
<211>  1725
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PDI-H3 Kan-S228Q DNA

<400>  121
atggcgaaaa acgttgcgat tttcggctta ttgttttctc ttcttgtgtt ggttccttct       60

cagatcttcg cgcaaaaaat ccctggaaat gacaatagca cggcaacgct gtgccttggg      120

caccatgcag taccaaacgg aacgatagtg aaaacaatca cgaatgaccg aattgaagtt      180

actaatgcta ctgagctggt tcagaattcc tcaataggtg aaatatgcga cagtcctcat      240

cagatccttg atggagaaaa ctgcacacta atagatgctc tattgggaga ccctcagtgt      300

gatggctttc aaaataagaa atgggacctt tttgttgaac gaaacaaagc ctacagcaac      360

tgttaccctt atgatgtgcc ggattatgcc tcccttaggt cactagttgc ctcatccggc      420

acactggagt ttaacaatga aagcttcaat tgggctggag tcactcaaaa cggaacaagt      480

tcttcttgca taaggggatc taagagtagt ttctttagta gattaaattg gttgacccac      540

ttaaactcca aatacccagc attaaacgtg actatgccaa acaatgaaca atttgacaaa      600

ttgtacattt ggggggttca ccacccgggt acggacaagg accaaatcag cctgtatgca      660

caatcatcag gaagaatcac agtatctacc aaaagaagcc aacaagctgt aatcccgaat      720

atcggatcta gacccagaat aagggatatc cctcagagaa taagcatcta ttggacaata      780

gtaaaaccgg gagacatact tttgattaac agcacaggga atctaattgc tcctaggggt      840

tacttcaaaa tacgaagtgg gaaaagctca ataatgagat cagatgcacc cattggcaaa      900

tgcaagtctg aatgcatcac tccaaatgga agcattccca atgacaaacc attccaaaat      960

gtaaacagga tcacatacgg ggcctgtccc agatatgtta agcaaagcac tctgaaattg     1020

gcaacaggaa tgcgaaatgt accagagaga caaactagag gcatatttgg cgcaatagcg     1080

ggtttcatag aaaatggttg ggagggaatg gtggatggtt ggtacggctt caggcatcaa     1140

aattctgagg gaagaggaca agcagcagat ctcaaaagca ctcaagcagc aatcgatcaa     1200

atcaatggga agctggctcg agttatcggg aaaaccaacg agaaattcca tcagattgaa     1260

aaagaattct cagaagtaga agggagaatt caggaccttg agaaatatgt tgaggacaca     1320

aaaatagatc tctggtcata caacgcggag cttcttgttg ccctggagaa ccaacataca     1380

attgatctaa ctgactcaga aatgaacaaa ctgtttgaaa aaacaaagaa gcaactgagg     1440

gaaaatgctg aggatatggg caatggttgt ttcaaaatat accacaaatg tgacaatgcc     1500

tgcatgggat caatcagaaa tggaacttat gaccacaacg tatacaggga tgaagcatta     1560

aacaaccggt tccagatcaa gggagttgag ctgaagtcag ggtacaaaga ttggatccta     1620

tggatttcct ttgccatatc atcccttgta ctgttagttg ctttgttggg gttcatcatg     1680

tgggcctgcc aaaagggcaa cattaggtgc aacatttgca tttga                     1725


<210>  122
<211>  574
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  PDI-H3 Kan-S228Q AA

<400>  122

Met Ala Lys Asn Val Ala Ile Phe Gly Leu Leu Phe Ser Leu Leu Val 
1               5                   10                  15      


Leu Val Pro Ser Gln Ile Phe Ala Gln Lys Ile Pro Gly Asn Asp Asn 
            20                  25                  30          


Ser Thr Ala Thr Leu Cys Leu Gly His His Ala Val Pro Asn Gly Thr 
        35                  40                  45              


Ile Val Lys Thr Ile Thr Asn Asp Arg Ile Glu Val Thr Asn Ala Thr 
    50                  55                  60                  


Glu Leu Val Gln Asn Ser Ser Ile Gly Glu Ile Cys Asp Ser Pro His 
65                  70                  75                  80  


Gln Ile Leu Asp Gly Glu Asn Cys Thr Leu Ile Asp Ala Leu Leu Gly 
                85                  90                  95      


Asp Pro Gln Cys Asp Gly Phe Gln Asn Lys Lys Trp Asp Leu Phe Val 
            100                 105                 110         


Glu Arg Asn Lys Ala Tyr Ser Asn Cys Tyr Pro Tyr Asp Val Pro Asp 
        115                 120                 125             


Tyr Ala Ser Leu Arg Ser Leu Val Ala Ser Ser Gly Thr Leu Glu Phe 
    130                 135                 140                 


Asn Asn Glu Ser Phe Asn Trp Ala Gly Val Thr Gln Asn Gly Thr Ser 
145                 150                 155                 160 


Ser Ser Cys Ile Arg Gly Ser Lys Ser Ser Phe Phe Ser Arg Leu Asn 
                165                 170                 175     


Trp Leu Thr His Leu Asn Ser Lys Tyr Pro Ala Leu Asn Val Thr Met 
            180                 185                 190         


Pro Asn Asn Glu Gln Phe Asp Lys Leu Tyr Ile Trp Gly Val His His 
        195                 200                 205             


Pro Gly Thr Asp Lys Asp Gln Ile Ser Leu Tyr Ala Gln Ser Ser Gly 
    210                 215                 220                 


Arg Ile Thr Val Ser Thr Lys Arg Ser Gln Gln Ala Val Ile Pro Asn 
225                 230                 235                 240 


Ile Gly Ser Arg Pro Arg Ile Arg Asp Ile Pro Gln Arg Ile Ser Ile 
                245                 250                 255     


Tyr Trp Thr Ile Val Lys Pro Gly Asp Ile Leu Leu Ile Asn Ser Thr 
            260                 265                 270         


Gly Asn Leu Ile Ala Pro Arg Gly Tyr Phe Lys Ile Arg Ser Gly Lys 
        275                 280                 285             


Ser Ser Ile Met Arg Ser Asp Ala Pro Ile Gly Lys Cys Lys Ser Glu 
    290                 295                 300                 


Cys Ile Thr Pro Asn Gly Ser Ile Pro Asn Asp Lys Pro Phe Gln Asn 
305                 310                 315                 320 


Val Asn Arg Ile Thr Tyr Gly Ala Cys Pro Arg Tyr Val Lys Gln Ser 
                325                 330                 335     


Thr Leu Lys Leu Ala Thr Gly Met Arg Asn Val Pro Glu Arg Gln Thr 
            340                 345                 350         


Arg Gly Ile Phe Gly Ala Ile Ala Gly Phe Ile Glu Asn Gly Trp Glu 
        355                 360                 365             


Gly Met Val Asp Gly Trp Tyr Gly Phe Arg His Gln Asn Ser Glu Gly 
    370                 375                 380                 


Arg Gly Gln Ala Ala Asp Leu Lys Ser Thr Gln Ala Ala Ile Asp Gln 
385                 390                 395                 400 


Ile Asn Gly Lys Leu Ala Arg Val Ile Gly Lys Thr Asn Glu Lys Phe 
                405                 410                 415     


His Gln Ile Glu Lys Glu Phe Ser Glu Val Glu Gly Arg Ile Gln Asp 
            420                 425                 430         


Leu Glu Lys Tyr Val Glu Asp Thr Lys Ile Asp Leu Trp Ser Tyr Asn 
        435                 440                 445             


Ala Glu Leu Leu Val Ala Leu Glu Asn Gln His Thr Ile Asp Leu Thr 
    450                 455                 460                 


Asp Ser Glu Met Asn Lys Leu Phe Glu Lys Thr Lys Lys Gln Leu Arg 
465                 470                 475                 480 


Glu Asn Ala Glu Asp Met Gly Asn Gly Cys Phe Lys Ile Tyr His Lys 
                485                 490                 495     


Cys Asp Asn Ala Cys Met Gly Ser Ile Arg Asn Gly Thr Tyr Asp His 
            500                 505                 510         


Asn Val Tyr Arg Asp Glu Ala Leu Asn Asn Arg Phe Gln Ile Lys Gly 
        515                 520                 525             


Val Glu Leu Lys Ser Gly Tyr Lys Asp Trp Ile Leu Trp Ile Ser Phe 
    530                 535                 540                 


Ala Ile Ser Ser Leu Val Leu Leu Val Ala Leu Leu Gly Phe Ile Met 
545                 550                 555                 560 


Trp Ala Cys Gln Lys Gly Asn Ile Arg Cys Asn Ile Cys Ile 
                565                 570                 


<210>  123
<211>  1731
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PDI-B Sing DNA

<400>  123
atggcgaaaa acgttgcgat tttcggctta ttgttttctc ttcttgtgtt ggttccttct       60

cagatcttcg cggatcgaat ctgcactggg ataacatctt caaactcacc tcatgtggtc      120

aaaacagcta ctcaagggga ggtcaatgtg actggcgtga taccactgac aacaacacca      180

acaaaatctt attttgcaaa tctcaaagga acaaggacca gagggaaact atgcccggac      240

tgtctcaact gtacagatct ggatgtggcc ttgggcaggc caatgtgtgt ggggaccaca      300

ccttctgcta aagcttcaat actccatgag gtcagacctg ttacatccgg gtgctttcct      360

ataatgcacg acagaacaaa aatcaggcaa ctacccaatc ttctcagagg atatgaaaag      420

atcaggttat caacccaaaa cgttatcgat gcagaaaaag caccaggagg accctacaga      480

cttggaacct caggatcttg ccctaacgct accagtaaaa tcggattttt tgcaacaatg      540

gcttgggctg tcccaaagga caactacaaa aatgcaacga acccacaaac agtggaagta      600

ccatacattt gtacagaagg ggaagaccaa attactgttt gggggttcca ttcggataac      660

aaaacccaaa tgaagagcct ctatggagac tcaaatcctc aaaagttcac ctcatctgct      720

aatggagtaa ccacgcatta tgtttctcag attggcgact tcccagatca aacagaagac      780

ggaggactac cacaaagcgg cagaattgtt gttgattaca tggtgcaaaa acctgggaaa      840

acaggaacaa ttgtctatca aaggggtgtt ttgttgcctc aaaaggtgtg gtgcgcgagt      900

ggcaggagca aagtaataaa agggtcattg cctttaattg gtgaagcaga ttgccttcat      960

gaagaatacg gtggattaaa caaaagcaag ccttactaca caggaaaaca tgcaaaagcc     1020

ataggaaatt gcccaatatg ggtaaaaaca cctttgaagc ttgccaatgg aaccaaatat     1080

agacctcctg gtggaggatg ggaaggaatg attgcaggtt ggcacggata cacatctcac     1140

ggagcacatg gagtggcagt ggcggcagac cttaagagta cacaagaagc tataaataag     1200

ataacaaaaa atctcaattc tttgagtgaa ctagaagtaa agaaccttca aagactaagt     1260

ggtgccatgg atgaactcca caacgaaata ctcgagctgg atgaaaaagt ggatgatctc     1320

agagctgaca ctataagctc acaaatagaa cttgcagtct tgctttccaa cgaaggaata     1380

ataaacagtg aagacgagca tctattggca cttgagagaa aactaaagaa aatgctgggt     1440

ccctctgctg tagacatagg aaacggatgc ttcgaaacca aacacaaatg caaccagacc     1500

tgcttagaca ggatagctgc tggcaccttt aatgcaggag aattttctct ccccactttt     1560

gattcattga acattactgc tgcatcttta aatgatgatg gattggataa ctaccagatt     1620

ttggcgatct attcaactgt cgccagttca ttggtactgg tagtctccct gggggcaatc     1680

agtttctgga tgtgctctaa tgggtctcta cagtgtagaa tatgtattta a              1731


<210>  124
<211>  576
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  PDI-B Sing AA

<400>  124

Met Ala Lys Asn Val Ala Ile Phe Gly Leu Leu Phe Ser Leu Leu Val 
1               5                   10                  15      


Leu Val Pro Ser Gln Ile Phe Ala Asp Arg Ile Cys Thr Gly Ile Thr 
            20                  25                  30          


Ser Ser Asn Ser Pro His Val Val Lys Thr Ala Thr Gln Gly Glu Val 
        35                  40                  45              


Asn Val Thr Gly Val Ile Pro Leu Thr Thr Thr Pro Thr Lys Ser Tyr 
    50                  55                  60                  


Phe Ala Asn Leu Lys Gly Thr Arg Thr Arg Gly Lys Leu Cys Pro Asp 
65                  70                  75                  80  


Cys Leu Asn Cys Thr Asp Leu Asp Val Ala Leu Gly Arg Pro Met Cys 
                85                  90                  95      


Val Gly Thr Thr Pro Ser Ala Lys Ala Ser Ile Leu His Glu Val Arg 
            100                 105                 110         


Pro Val Thr Ser Gly Cys Phe Pro Ile Met His Asp Arg Thr Lys Ile 
        115                 120                 125             


Arg Gln Leu Pro Asn Leu Leu Arg Gly Tyr Glu Lys Ile Arg Leu Ser 
    130                 135                 140                 


Thr Gln Asn Val Ile Asp Ala Glu Lys Ala Pro Gly Gly Pro Tyr Arg 
145                 150                 155                 160 


Leu Gly Thr Ser Gly Ser Cys Pro Asn Ala Thr Ser Lys Ile Gly Phe 
                165                 170                 175     


Phe Ala Thr Met Ala Trp Ala Val Pro Lys Asp Asn Tyr Lys Asn Ala 
            180                 185                 190         


Thr Asn Pro Gln Thr Val Glu Val Pro Tyr Ile Cys Thr Glu Gly Glu 
        195                 200                 205             


Asp Gln Ile Thr Val Trp Gly Phe His Ser Asp Asn Lys Thr Gln Met 
    210                 215                 220                 


Lys Ser Leu Tyr Gly Asp Ser Asn Pro Gln Lys Phe Thr Ser Ser Ala 
225                 230                 235                 240 


Asn Gly Val Thr Thr His Tyr Val Ser Gln Ile Gly Asp Phe Pro Asp 
                245                 250                 255     


Gln Thr Glu Asp Gly Gly Leu Pro Gln Ser Gly Arg Ile Val Val Asp 
            260                 265                 270         


Tyr Met Val Gln Lys Pro Gly Lys Thr Gly Thr Ile Val Tyr Gln Arg 
        275                 280                 285             


Gly Val Leu Leu Pro Gln Lys Val Trp Cys Ala Ser Gly Arg Ser Lys 
    290                 295                 300                 


Val Ile Lys Gly Ser Leu Pro Leu Ile Gly Glu Ala Asp Cys Leu His 
305                 310                 315                 320 


Glu Glu Tyr Gly Gly Leu Asn Lys Ser Lys Pro Tyr Tyr Thr Gly Lys 
                325                 330                 335     


His Ala Lys Ala Ile Gly Asn Cys Pro Ile Trp Val Lys Thr Pro Leu 
            340                 345                 350         


Lys Leu Ala Asn Gly Thr Lys Tyr Arg Pro Pro Gly Gly Gly Trp Glu 
        355                 360                 365             


Gly Met Ile Ala Gly Trp His Gly Tyr Thr Ser His Gly Ala His Gly 
    370                 375                 380                 


Val Ala Val Ala Ala Asp Leu Lys Ser Thr Gln Glu Ala Ile Asn Lys 
385                 390                 395                 400 


Ile Thr Lys Asn Leu Asn Ser Leu Ser Glu Leu Glu Val Lys Asn Leu 
                405                 410                 415     


Gln Arg Leu Ser Gly Ala Met Asp Glu Leu His Asn Glu Ile Leu Glu 
            420                 425                 430         


Leu Asp Glu Lys Val Asp Asp Leu Arg Ala Asp Thr Ile Ser Ser Gln 
        435                 440                 445             


Ile Glu Leu Ala Val Leu Leu Ser Asn Glu Gly Ile Ile Asn Ser Glu 
    450                 455                 460                 


Asp Glu His Leu Leu Ala Leu Glu Arg Lys Leu Lys Lys Met Leu Gly 
465                 470                 475                 480 


Pro Ser Ala Val Asp Ile Gly Asn Gly Cys Phe Glu Thr Lys His Lys 
                485                 490                 495     


Cys Asn Gln Thr Cys Leu Asp Arg Ile Ala Ala Gly Thr Phe Asn Ala 
            500                 505                 510         


Gly Glu Phe Ser Leu Pro Thr Phe Asp Ser Leu Asn Ile Thr Ala Ala 
        515                 520                 525             


Ser Leu Asn Asp Asp Gly Leu Asp Asn Tyr Gln Ile Leu Ala Ile Tyr 
    530                 535                 540                 


Ser Thr Val Ala Ser Ser Leu Val Leu Val Val Ser Leu Gly Ala Ile 
545                 550                 555                 560 


Ser Phe Trp Met Cys Ser Asn Gly Ser Leu Gln Cys Arg Ile Cys Ile 
                565                 570                 575     


<210>  125
<211>  1731
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PDI-B Sing-G138A DNA

<400>  125
atggcgaaaa acgttgcgat tttcggctta ttgttttctc ttcttgtgtt ggttccttct       60

cagatcttcg cggatcgaat ctgcactggg ataacatctt caaactcacc tcatgtggtc      120

aaaacagcta ctcaagggga ggtcaatgtg actggcgtga taccactgac aacaacacca      180

acaaaatctt attttgcaaa tctcaaagga acaaggacca gagggaaact atgcccggac      240

tgtctcaact gtacagatct ggatgtggcc ttgggcaggc caatgtgtgt ggggaccaca      300

ccttctgcta aagcttcaat actccatgag gtcagacctg ttacatccgg gtgctttcct      360

ataatgcacg acagaacaaa aatcaggcaa ctacccaatc ttctcagagg atatgaaaag      420

atcaggttat caacccaaaa cgttatcgat gcagaaaaag caccaggagg accctacaga      480

cttgccacct caggatcttg ccctaacgct accagtaaaa tcggattttt tgcaacaatg      540

gcttgggctg tcccaaagga caactacaaa aatgcaacga acccacaaac agtggaagta      600

ccatacattt gtacagaagg ggaagaccaa attactgttt gggggttcca ttcggataac      660

aaaacccaaa tgaagagcct ctatggagac tcaaatcctc aaaagttcac ctcatctgct      720

aatggagtaa ccacgcatta tgtttctcag attggcgact tcccagatca aacagaagac      780

ggaggactac cacaaagcgg cagaattgtt gttgattaca tggtgcaaaa acctgggaaa      840

acaggaacaa ttgtctatca aaggggtgtt ttgttgcctc aaaaggtgtg gtgcgcgagt      900

ggcaggagca aagtaataaa agggtcattg cctttaattg gtgaagcaga ttgccttcat      960

gaagaatacg gtggattaaa caaaagcaag ccttactaca caggaaaaca tgcaaaagcc     1020

ataggaaatt gcccaatatg ggtaaaaaca cctttgaagc ttgccaatgg aaccaaatat     1080

agacctcctg gtggaggatg ggaaggaatg attgcaggtt ggcacggata cacatctcac     1140

ggagcacatg gagtggcagt ggcggcagac cttaagagta cacaagaagc tataaataag     1200

ataacaaaaa atctcaattc tttgagtgaa ctagaagtaa agaaccttca aagactaagt     1260

ggtgccatgg atgaactcca caacgaaata ctcgagctgg atgaaaaagt ggatgatctc     1320

agagctgaca ctataagctc acaaatagaa cttgcagtct tgctttccaa cgaaggaata     1380

ataaacagtg aagacgagca tctattggca cttgagagaa aactaaagaa aatgctgggt     1440

ccctctgctg tagacatagg aaacggatgc ttcgaaacca aacacaaatg caaccagacc     1500

tgcttagaca ggatagctgc tggcaccttt aatgcaggag aattttctct ccccactttt     1560

gattcattga acattactgc tgcatcttta aatgatgatg gattggataa ctaccagatt     1620

ttggcgatct attcaactgt cgccagttca ttggtactgg tagtctccct gggggcaatc     1680

agtttctgga tgtgctctaa tgggtctcta cagtgtagaa tatgtattta a              1731


<210>  126
<211>  576
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  PDI-B Sing-G138A AA

<400>  126

Met Ala Lys Asn Val Ala Ile Phe Gly Leu Leu Phe Ser Leu Leu Val 
1               5                   10                  15      


Leu Val Pro Ser Gln Ile Phe Ala Asp Arg Ile Cys Thr Gly Ile Thr 
            20                  25                  30          


Ser Ser Asn Ser Pro His Val Val Lys Thr Ala Thr Gln Gly Glu Val 
        35                  40                  45              


Asn Val Thr Gly Val Ile Pro Leu Thr Thr Thr Pro Thr Lys Ser Tyr 
    50                  55                  60                  


Phe Ala Asn Leu Lys Gly Thr Arg Thr Arg Gly Lys Leu Cys Pro Asp 
65                  70                  75                  80  


Cys Leu Asn Cys Thr Asp Leu Asp Val Ala Leu Gly Arg Pro Met Cys 
                85                  90                  95      


Val Gly Thr Thr Pro Ser Ala Lys Ala Ser Ile Leu His Glu Val Arg 
            100                 105                 110         


Pro Val Thr Ser Gly Cys Phe Pro Ile Met His Asp Arg Thr Lys Ile 
        115                 120                 125             


Arg Gln Leu Pro Asn Leu Leu Arg Gly Tyr Glu Lys Ile Arg Leu Ser 
    130                 135                 140                 


Thr Gln Asn Val Ile Asp Ala Glu Lys Ala Pro Gly Gly Pro Tyr Arg 
145                 150                 155                 160 


Leu Ala Thr Ser Gly Ser Cys Pro Asn Ala Thr Ser Lys Ile Gly Phe 
                165                 170                 175     


Phe Ala Thr Met Ala Trp Ala Val Pro Lys Asp Asn Tyr Lys Asn Ala 
            180                 185                 190         


Thr Asn Pro Gln Thr Val Glu Val Pro Tyr Ile Cys Thr Glu Gly Glu 
        195                 200                 205             


Asp Gln Ile Thr Val Trp Gly Phe His Ser Asp Asn Lys Thr Gln Met 
    210                 215                 220                 


Lys Ser Leu Tyr Gly Asp Ser Asn Pro Gln Lys Phe Thr Ser Ser Ala 
225                 230                 235                 240 


Asn Gly Val Thr Thr His Tyr Val Ser Gln Ile Gly Asp Phe Pro Asp 
                245                 250                 255     


Gln Thr Glu Asp Gly Gly Leu Pro Gln Ser Gly Arg Ile Val Val Asp 
            260                 265                 270         


Tyr Met Val Gln Lys Pro Gly Lys Thr Gly Thr Ile Val Tyr Gln Arg 
        275                 280                 285             


Gly Val Leu Leu Pro Gln Lys Val Trp Cys Ala Ser Gly Arg Ser Lys 
    290                 295                 300                 


Val Ile Lys Gly Ser Leu Pro Leu Ile Gly Glu Ala Asp Cys Leu His 
305                 310                 315                 320 


Glu Glu Tyr Gly Gly Leu Asn Lys Ser Lys Pro Tyr Tyr Thr Gly Lys 
                325                 330                 335     


His Ala Lys Ala Ile Gly Asn Cys Pro Ile Trp Val Lys Thr Pro Leu 
            340                 345                 350         


Lys Leu Ala Asn Gly Thr Lys Tyr Arg Pro Pro Gly Gly Gly Trp Glu 
        355                 360                 365             


Gly Met Ile Ala Gly Trp His Gly Tyr Thr Ser His Gly Ala His Gly 
    370                 375                 380                 


Val Ala Val Ala Ala Asp Leu Lys Ser Thr Gln Glu Ala Ile Asn Lys 
385                 390                 395                 400 


Ile Thr Lys Asn Leu Asn Ser Leu Ser Glu Leu Glu Val Lys Asn Leu 
                405                 410                 415     


Gln Arg Leu Ser Gly Ala Met Asp Glu Leu His Asn Glu Ile Leu Glu 
            420                 425                 430         


Leu Asp Glu Lys Val Asp Asp Leu Arg Ala Asp Thr Ile Ser Ser Gln 
        435                 440                 445             


Ile Glu Leu Ala Val Leu Leu Ser Asn Glu Gly Ile Ile Asn Ser Glu 
    450                 455                 460                 


Asp Glu His Leu Leu Ala Leu Glu Arg Lys Leu Lys Lys Met Leu Gly 
465                 470                 475                 480 


Pro Ser Ala Val Asp Ile Gly Asn Gly Cys Phe Glu Thr Lys His Lys 
                485                 490                 495     


Cys Asn Gln Thr Cys Leu Asp Arg Ile Ala Ala Gly Thr Phe Asn Ala 
            500                 505                 510         


Gly Glu Phe Ser Leu Pro Thr Phe Asp Ser Leu Asn Ile Thr Ala Ala 
        515                 520                 525             


Ser Leu Asn Asp Asp Gly Leu Asp Asn Tyr Gln Ile Leu Ala Ile Tyr 
    530                 535                 540                 


Ser Thr Val Ala Ser Ser Leu Val Leu Val Val Ser Leu Gly Ala Ile 
545                 550                 555                 560 


Ser Phe Trp Met Cys Ser Asn Gly Ser Leu Gln Cys Arg Ile Cys Ile 
                565                 570                 575     


<210>  127
<211>  1731
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PDI-B Sing-S140A DNA

<400>  127
atggcgaaaa acgttgcgat tttcggctta ttgttttctc ttcttgtgtt ggttccttct       60

cagatcttcg cggatcgaat ctgcactggg ataacatctt caaactcacc tcatgtggtc      120

aaaacagcta ctcaagggga ggtcaatgtg actggcgtga taccactgac aacaacacca      180

acaaaatctt attttgcaaa tctcaaagga acaaggacca gagggaaact atgcccggac      240

tgtctcaact gtacagatct ggatgtggcc ttgggcaggc caatgtgtgt ggggaccaca      300

ccttctgcta aagcttcaat actccatgag gtcagacctg ttacatccgg gtgctttcct      360

ataatgcacg acagaacaaa aatcaggcaa ctacccaatc ttctcagagg atatgaaaag      420

atcaggttat caacccaaaa cgttatcgat gcagaaaaag caccaggagg accctacaga      480

cttggaaccg ccggatcttg ccctaacgct accagtaaaa tcggattttt tgcaacaatg      540

gcttgggctg tcccaaagga caactacaaa aatgcaacga acccacaaac agtggaagta      600

ccatacattt gtacagaagg ggaagaccaa attactgttt gggggttcca ttcggataac      660

aaaacccaaa tgaagagcct ctatggagac tcaaatcctc aaaagttcac ctcatctgct      720

aatggagtaa ccacgcatta tgtttctcag attggcgact tcccagatca aacagaagac      780

ggaggactac cacaaagcgg cagaattgtt gttgattaca tggtgcaaaa acctgggaaa      840

acaggaacaa ttgtctatca aaggggtgtt ttgttgcctc aaaaggtgtg gtgcgcgagt      900

ggcaggagca aagtaataaa agggtcattg cctttaattg gtgaagcaga ttgccttcat      960

gaagaatacg gtggattaaa caaaagcaag ccttactaca caggaaaaca tgcaaaagcc     1020

ataggaaatt gcccaatatg ggtaaaaaca cctttgaagc ttgccaatgg aaccaaatat     1080

agacctcctg gtggaggatg ggaaggaatg attgcaggtt ggcacggata cacatctcac     1140

ggagcacatg gagtggcagt ggcggcagac cttaagagta cacaagaagc tataaataag     1200

ataacaaaaa atctcaattc tttgagtgaa ctagaagtaa agaaccttca aagactaagt     1260

ggtgccatgg atgaactcca caacgaaata ctcgagctgg atgaaaaagt ggatgatctc     1320

agagctgaca ctataagctc acaaatagaa cttgcagtct tgctttccaa cgaaggaata     1380

ataaacagtg aagacgagca tctattggca cttgagagaa aactaaagaa aatgctgggt     1440

ccctctgctg tagacatagg aaacggatgc ttcgaaacca aacacaaatg caaccagacc     1500

tgcttagaca ggatagctgc tggcaccttt aatgcaggag aattttctct ccccactttt     1560

gattcattga acattactgc tgcatcttta aatgatgatg gattggataa ctaccagatt     1620

ttggcgatct attcaactgt cgccagttca ttggtactgg tagtctccct gggggcaatc     1680

agtttctgga tgtgctctaa tgggtctcta cagtgtagaa tatgtattta a              1731


<210>  128
<211>  576
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  PDI-B Sing-S140A AA

<400>  128

Met Ala Lys Asn Val Ala Ile Phe Gly Leu Leu Phe Ser Leu Leu Val 
1               5                   10                  15      


Leu Val Pro Ser Gln Ile Phe Ala Asp Arg Ile Cys Thr Gly Ile Thr 
            20                  25                  30          


Ser Ser Asn Ser Pro His Val Val Lys Thr Ala Thr Gln Gly Glu Val 
        35                  40                  45              


Asn Val Thr Gly Val Ile Pro Leu Thr Thr Thr Pro Thr Lys Ser Tyr 
    50                  55                  60                  


Phe Ala Asn Leu Lys Gly Thr Arg Thr Arg Gly Lys Leu Cys Pro Asp 
65                  70                  75                  80  


Cys Leu Asn Cys Thr Asp Leu Asp Val Ala Leu Gly Arg Pro Met Cys 
                85                  90                  95      


Val Gly Thr Thr Pro Ser Ala Lys Ala Ser Ile Leu His Glu Val Arg 
            100                 105                 110         


Pro Val Thr Ser Gly Cys Phe Pro Ile Met His Asp Arg Thr Lys Ile 
        115                 120                 125             


Arg Gln Leu Pro Asn Leu Leu Arg Gly Tyr Glu Lys Ile Arg Leu Ser 
    130                 135                 140                 


Thr Gln Asn Val Ile Asp Ala Glu Lys Ala Pro Gly Gly Pro Tyr Arg 
145                 150                 155                 160 


Leu Gly Thr Ala Gly Ser Cys Pro Asn Ala Thr Ser Lys Ile Gly Phe 
                165                 170                 175     


Phe Ala Thr Met Ala Trp Ala Val Pro Lys Asp Asn Tyr Lys Asn Ala 
            180                 185                 190         


Thr Asn Pro Gln Thr Val Glu Val Pro Tyr Ile Cys Thr Glu Gly Glu 
        195                 200                 205             


Asp Gln Ile Thr Val Trp Gly Phe His Ser Asp Asn Lys Thr Gln Met 
    210                 215                 220                 


Lys Ser Leu Tyr Gly Asp Ser Asn Pro Gln Lys Phe Thr Ser Ser Ala 
225                 230                 235                 240 


Asn Gly Val Thr Thr His Tyr Val Ser Gln Ile Gly Asp Phe Pro Asp 
                245                 250                 255     


Gln Thr Glu Asp Gly Gly Leu Pro Gln Ser Gly Arg Ile Val Val Asp 
            260                 265                 270         


Tyr Met Val Gln Lys Pro Gly Lys Thr Gly Thr Ile Val Tyr Gln Arg 
        275                 280                 285             


Gly Val Leu Leu Pro Gln Lys Val Trp Cys Ala Ser Gly Arg Ser Lys 
    290                 295                 300                 


Val Ile Lys Gly Ser Leu Pro Leu Ile Gly Glu Ala Asp Cys Leu His 
305                 310                 315                 320 


Glu Glu Tyr Gly Gly Leu Asn Lys Ser Lys Pro Tyr Tyr Thr Gly Lys 
                325                 330                 335     


His Ala Lys Ala Ile Gly Asn Cys Pro Ile Trp Val Lys Thr Pro Leu 
            340                 345                 350         


Lys Leu Ala Asn Gly Thr Lys Tyr Arg Pro Pro Gly Gly Gly Trp Glu 
        355                 360                 365             


Gly Met Ile Ala Gly Trp His Gly Tyr Thr Ser His Gly Ala His Gly 
    370                 375                 380                 


Val Ala Val Ala Ala Asp Leu Lys Ser Thr Gln Glu Ala Ile Asn Lys 
385                 390                 395                 400 


Ile Thr Lys Asn Leu Asn Ser Leu Ser Glu Leu Glu Val Lys Asn Leu 
                405                 410                 415     


Gln Arg Leu Ser Gly Ala Met Asp Glu Leu His Asn Glu Ile Leu Glu 
            420                 425                 430         


Leu Asp Glu Lys Val Asp Asp Leu Arg Ala Asp Thr Ile Ser Ser Gln 
        435                 440                 445             


Ile Glu Leu Ala Val Leu Leu Ser Asn Glu Gly Ile Ile Asn Ser Glu 
    450                 455                 460                 


Asp Glu His Leu Leu Ala Leu Glu Arg Lys Leu Lys Lys Met Leu Gly 
465                 470                 475                 480 


Pro Ser Ala Val Asp Ile Gly Asn Gly Cys Phe Glu Thr Lys His Lys 
                485                 490                 495     


Cys Asn Gln Thr Cys Leu Asp Arg Ile Ala Ala Gly Thr Phe Asn Ala 
            500                 505                 510         


Gly Glu Phe Ser Leu Pro Thr Phe Asp Ser Leu Asn Ile Thr Ala Ala 
        515                 520                 525             


Ser Leu Asn Asp Asp Gly Leu Asp Asn Tyr Gln Ile Leu Ala Ile Tyr 
    530                 535                 540                 


Ser Thr Val Ala Ser Ser Leu Val Leu Val Val Ser Leu Gly Ala Ile 
545                 550                 555                 560 


Ser Phe Trp Met Cys Ser Asn Gly Ser Leu Gln Cys Arg Ile Cys Ile 
                565                 570                 575     


<210>  129
<211>  1731
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PDI-B Sing-S142A DNA

<400>  129
atggcgaaaa acgttgcgat tttcggctta ttgttttctc ttcttgtgtt ggttccttct       60

cagatcttcg cggatcgaat ctgcactggg ataacatctt caaactcacc tcatgtggtc      120

aaaacagcta ctcaagggga ggtcaatgtg actggcgtga taccactgac aacaacacca      180

acaaaatctt attttgcaaa tctcaaagga acaaggacca gagggaaact atgcccggac      240

tgtctcaact gtacagatct ggatgtggcc ttgggcaggc caatgtgtgt ggggaccaca      300

ccttctgcta aagcttcaat actccatgag gtcagacctg ttacatccgg gtgctttcct      360

ataatgcacg acagaacaaa aatcaggcaa ctacccaatc ttctcagagg atatgaaaag      420

atcaggttat caacccaaaa cgttatcgat gcagaaaaag caccaggagg accctacaga      480

cttggaacct caggagcctg ccctaacgct accagtaaaa tcggattttt tgcaacaatg      540

gcttgggctg tcccaaagga caactacaaa aatgcaacga acccacaaac agtggaagta      600

ccatacattt gtacagaagg ggaagaccaa attactgttt gggggttcca ttcggataac      660

aaaacccaaa tgaagagcct ctatggagac tcaaatcctc aaaagttcac ctcatctgct      720

aatggagtaa ccacgcatta tgtttctcag attggcgact tcccagatca aacagaagac      780

ggaggactac cacaaagcgg cagaattgtt gttgattaca tggtgcaaaa acctgggaaa      840

acaggaacaa ttgtctatca aaggggtgtt ttgttgcctc aaaaggtgtg gtgcgcgagt      900

ggcaggagca aagtaataaa agggtcattg cctttaattg gtgaagcaga ttgccttcat      960

gaagaatacg gtggattaaa caaaagcaag ccttactaca caggaaaaca tgcaaaagcc     1020

ataggaaatt gcccaatatg ggtaaaaaca cctttgaagc ttgccaatgg aaccaaatat     1080

agacctcctg gtggaggatg ggaaggaatg attgcaggtt ggcacggata cacatctcac     1140

ggagcacatg gagtggcagt ggcggcagac cttaagagta cacaagaagc tataaataag     1200

ataacaaaaa atctcaattc tttgagtgaa ctagaagtaa agaaccttca aagactaagt     1260

ggtgccatgg atgaactcca caacgaaata ctcgagctgg atgaaaaagt ggatgatctc     1320

agagctgaca ctataagctc acaaatagaa cttgcagtct tgctttccaa cgaaggaata     1380

ataaacagtg aagacgagca tctattggca cttgagagaa aactaaagaa aatgctgggt     1440

ccctctgctg tagacatagg aaacggatgc ttcgaaacca aacacaaatg caaccagacc     1500

tgcttagaca ggatagctgc tggcaccttt aatgcaggag aattttctct ccccactttt     1560

gattcattga acattactgc tgcatcttta aatgatgatg gattggataa ctaccagatt     1620

ttggcgatct attcaactgt cgccagttca ttggtactgg tagtctccct gggggcaatc     1680

agtttctgga tgtgctctaa tgggtctcta cagtgtagaa tatgtattta a              1731


<210>  130
<211>  576
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  PDI-B Sing-S142A AA

<400>  130

Met Ala Lys Asn Val Ala Ile Phe Gly Leu Leu Phe Ser Leu Leu Val 
1               5                   10                  15      


Leu Val Pro Ser Gln Ile Phe Ala Asp Arg Ile Cys Thr Gly Ile Thr 
            20                  25                  30          


Ser Ser Asn Ser Pro His Val Val Lys Thr Ala Thr Gln Gly Glu Val 
        35                  40                  45              


Asn Val Thr Gly Val Ile Pro Leu Thr Thr Thr Pro Thr Lys Ser Tyr 
    50                  55                  60                  


Phe Ala Asn Leu Lys Gly Thr Arg Thr Arg Gly Lys Leu Cys Pro Asp 
65                  70                  75                  80  


Cys Leu Asn Cys Thr Asp Leu Asp Val Ala Leu Gly Arg Pro Met Cys 
                85                  90                  95      


Val Gly Thr Thr Pro Ser Ala Lys Ala Ser Ile Leu His Glu Val Arg 
            100                 105                 110         


Pro Val Thr Ser Gly Cys Phe Pro Ile Met His Asp Arg Thr Lys Ile 
        115                 120                 125             


Arg Gln Leu Pro Asn Leu Leu Arg Gly Tyr Glu Lys Ile Arg Leu Ser 
    130                 135                 140                 


Thr Gln Asn Val Ile Asp Ala Glu Lys Ala Pro Gly Gly Pro Tyr Arg 
145                 150                 155                 160 


Leu Gly Thr Ser Gly Ala Cys Pro Asn Ala Thr Ser Lys Ile Gly Phe 
                165                 170                 175     


Phe Ala Thr Met Ala Trp Ala Val Pro Lys Asp Asn Tyr Lys Asn Ala 
            180                 185                 190         


Thr Asn Pro Gln Thr Val Glu Val Pro Tyr Ile Cys Thr Glu Gly Glu 
        195                 200                 205             


Asp Gln Ile Thr Val Trp Gly Phe His Ser Asp Asn Lys Thr Gln Met 
    210                 215                 220                 


Lys Ser Leu Tyr Gly Asp Ser Asn Pro Gln Lys Phe Thr Ser Ser Ala 
225                 230                 235                 240 


Asn Gly Val Thr Thr His Tyr Val Ser Gln Ile Gly Asp Phe Pro Asp 
                245                 250                 255     


Gln Thr Glu Asp Gly Gly Leu Pro Gln Ser Gly Arg Ile Val Val Asp 
            260                 265                 270         


Tyr Met Val Gln Lys Pro Gly Lys Thr Gly Thr Ile Val Tyr Gln Arg 
        275                 280                 285             


Gly Val Leu Leu Pro Gln Lys Val Trp Cys Ala Ser Gly Arg Ser Lys 
    290                 295                 300                 


Val Ile Lys Gly Ser Leu Pro Leu Ile Gly Glu Ala Asp Cys Leu His 
305                 310                 315                 320 


Glu Glu Tyr Gly Gly Leu Asn Lys Ser Lys Pro Tyr Tyr Thr Gly Lys 
                325                 330                 335     


His Ala Lys Ala Ile Gly Asn Cys Pro Ile Trp Val Lys Thr Pro Leu 
            340                 345                 350         


Lys Leu Ala Asn Gly Thr Lys Tyr Arg Pro Pro Gly Gly Gly Trp Glu 
        355                 360                 365             


Gly Met Ile Ala Gly Trp His Gly Tyr Thr Ser His Gly Ala His Gly 
    370                 375                 380                 


Val Ala Val Ala Ala Asp Leu Lys Ser Thr Gln Glu Ala Ile Asn Lys 
385                 390                 395                 400 


Ile Thr Lys Asn Leu Asn Ser Leu Ser Glu Leu Glu Val Lys Asn Leu 
                405                 410                 415     


Gln Arg Leu Ser Gly Ala Met Asp Glu Leu His Asn Glu Ile Leu Glu 
            420                 425                 430         


Leu Asp Glu Lys Val Asp Asp Leu Arg Ala Asp Thr Ile Ser Ser Gln 
        435                 440                 445             


Ile Glu Leu Ala Val Leu Leu Ser Asn Glu Gly Ile Ile Asn Ser Glu 
    450                 455                 460                 


Asp Glu His Leu Leu Ala Leu Glu Arg Lys Leu Lys Lys Met Leu Gly 
465                 470                 475                 480 


Pro Ser Ala Val Asp Ile Gly Asn Gly Cys Phe Glu Thr Lys His Lys 
                485                 490                 495     


Cys Asn Gln Thr Cys Leu Asp Arg Ile Ala Ala Gly Thr Phe Asn Ala 
            500                 505                 510         


Gly Glu Phe Ser Leu Pro Thr Phe Asp Ser Leu Asn Ile Thr Ala Ala 
        515                 520                 525             


Ser Leu Asn Asp Asp Gly Leu Asp Asn Tyr Gln Ile Leu Ala Ile Tyr 
    530                 535                 540                 


Ser Thr Val Ala Ser Ser Leu Val Leu Val Val Ser Leu Gly Ala Ile 
545                 550                 555                 560 


Ser Phe Trp Met Cys Ser Asn Gly Ser Leu Gln Cys Arg Ile Cys Ile 
                565                 570                 575     


<210>  131
<211>  1731
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PDI-B Sing-D195G DNA

<400>  131
atggcgaaaa acgttgcgat tttcggctta ttgttttctc ttcttgtgtt ggttccttct       60

cagatcttcg cggatcgaat ctgcactggg ataacatctt caaactcacc tcatgtggtc      120

aaaacagcta ctcaagggga ggtcaatgtg actggcgtga taccactgac aacaacacca      180

acaaaatctt attttgcaaa tctcaaagga acaaggacca gagggaaact atgcccggac      240

tgtctcaact gtacagatct ggatgtggcc ttgggcaggc caatgtgtgt ggggaccaca      300

ccttctgcta aagcttcaat actccatgag gtcagacctg ttacatccgg gtgctttcct      360

ataatgcacg acagaacaaa aatcaggcaa ctacccaatc ttctcagagg atatgaaaag      420

atcaggttat caacccaaaa cgttatcgat gcagaaaaag caccaggagg accctacaga      480

cttggaacct caggatcttg ccctaacgct accagtaaaa tcggattttt tgcaacaatg      540

gcttgggctg tcccaaagga caactacaaa aatgcaacga acccacaaac agtggaagta      600

ccatacattt gtacagaagg ggaagaccaa attactgttt gggggttcca ttcgggcaac      660

aaaacccaaa tgaagagcct ctatggagac tcaaatcctc aaaagttcac ctcatctgct      720

aatggagtaa ccacgcatta tgtttctcag attggcgact tcccagatca aacagaagac      780

ggaggactac cacaaagcgg cagaattgtt gttgattaca tggtgcaaaa acctgggaaa      840

acaggaacaa ttgtctatca aaggggtgtt ttgttgcctc aaaaggtgtg gtgcgcgagt      900

ggcaggagca aagtaataaa agggtcattg cctttaattg gtgaagcaga ttgccttcat      960

gaagaatacg gtggattaaa caaaagcaag ccttactaca caggaaaaca tgcaaaagcc     1020

ataggaaatt gcccaatatg ggtaaaaaca cctttgaagc ttgccaatgg aaccaaatat     1080

agacctcctg gtggaggatg ggaaggaatg attgcaggtt ggcacggata cacatctcac     1140

ggagcacatg gagtggcagt ggcggcagac cttaagagta cacaagaagc tataaataag     1200

ataacaaaaa atctcaattc tttgagtgaa ctagaagtaa agaaccttca aagactaagt     1260

ggtgccatgg atgaactcca caacgaaata ctcgagctgg atgaaaaagt ggatgatctc     1320

agagctgaca ctataagctc acaaatagaa cttgcagtct tgctttccaa cgaaggaata     1380

ataaacagtg aagacgagca tctattggca cttgagagaa aactaaagaa aatgctgggt     1440

ccctctgctg tagacatagg aaacggatgc ttcgaaacca aacacaaatg caaccagacc     1500

tgcttagaca ggatagctgc tggcaccttt aatgcaggag aattttctct ccccactttt     1560

gattcattga acattactgc tgcatcttta aatgatgatg gattggataa ctaccagatt     1620

ttggcgatct attcaactgt cgccagttca ttggtactgg tagtctccct gggggcaatc     1680

agtttctgga tgtgctctaa tgggtctcta cagtgtagaa tatgtattta a              1731


<210>  132
<211>  576
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  PDI-B Sing-D195G AA

<400>  132

Met Ala Lys Asn Val Ala Ile Phe Gly Leu Leu Phe Ser Leu Leu Val 
1               5                   10                  15      


Leu Val Pro Ser Gln Ile Phe Ala Asp Arg Ile Cys Thr Gly Ile Thr 
            20                  25                  30          


Ser Ser Asn Ser Pro His Val Val Lys Thr Ala Thr Gln Gly Glu Val 
        35                  40                  45              


Asn Val Thr Gly Val Ile Pro Leu Thr Thr Thr Pro Thr Lys Ser Tyr 
    50                  55                  60                  


Phe Ala Asn Leu Lys Gly Thr Arg Thr Arg Gly Lys Leu Cys Pro Asp 
65                  70                  75                  80  


Cys Leu Asn Cys Thr Asp Leu Asp Val Ala Leu Gly Arg Pro Met Cys 
                85                  90                  95      


Val Gly Thr Thr Pro Ser Ala Lys Ala Ser Ile Leu His Glu Val Arg 
            100                 105                 110         


Pro Val Thr Ser Gly Cys Phe Pro Ile Met His Asp Arg Thr Lys Ile 
        115                 120                 125             


Arg Gln Leu Pro Asn Leu Leu Arg Gly Tyr Glu Lys Ile Arg Leu Ser 
    130                 135                 140                 


Thr Gln Asn Val Ile Asp Ala Glu Lys Ala Pro Gly Gly Pro Tyr Arg 
145                 150                 155                 160 


Leu Gly Thr Ser Gly Ser Cys Pro Asn Ala Thr Ser Lys Ile Gly Phe 
                165                 170                 175     


Phe Ala Thr Met Ala Trp Ala Val Pro Lys Asp Asn Tyr Lys Asn Ala 
            180                 185                 190         


Thr Asn Pro Gln Thr Val Glu Val Pro Tyr Ile Cys Thr Glu Gly Glu 
        195                 200                 205             


Asp Gln Ile Thr Val Trp Gly Phe His Ser Gly Asn Lys Thr Gln Met 
    210                 215                 220                 


Lys Ser Leu Tyr Gly Asp Ser Asn Pro Gln Lys Phe Thr Ser Ser Ala 
225                 230                 235                 240 


Asn Gly Val Thr Thr His Tyr Val Ser Gln Ile Gly Asp Phe Pro Asp 
                245                 250                 255     


Gln Thr Glu Asp Gly Gly Leu Pro Gln Ser Gly Arg Ile Val Val Asp 
            260                 265                 270         


Tyr Met Val Gln Lys Pro Gly Lys Thr Gly Thr Ile Val Tyr Gln Arg 
        275                 280                 285             


Gly Val Leu Leu Pro Gln Lys Val Trp Cys Ala Ser Gly Arg Ser Lys 
    290                 295                 300                 


Val Ile Lys Gly Ser Leu Pro Leu Ile Gly Glu Ala Asp Cys Leu His 
305                 310                 315                 320 


Glu Glu Tyr Gly Gly Leu Asn Lys Ser Lys Pro Tyr Tyr Thr Gly Lys 
                325                 330                 335     


His Ala Lys Ala Ile Gly Asn Cys Pro Ile Trp Val Lys Thr Pro Leu 
            340                 345                 350         


Lys Leu Ala Asn Gly Thr Lys Tyr Arg Pro Pro Gly Gly Gly Trp Glu 
        355                 360                 365             


Gly Met Ile Ala Gly Trp His Gly Tyr Thr Ser His Gly Ala His Gly 
    370                 375                 380                 


Val Ala Val Ala Ala Asp Leu Lys Ser Thr Gln Glu Ala Ile Asn Lys 
385                 390                 395                 400 


Ile Thr Lys Asn Leu Asn Ser Leu Ser Glu Leu Glu Val Lys Asn Leu 
                405                 410                 415     


Gln Arg Leu Ser Gly Ala Met Asp Glu Leu His Asn Glu Ile Leu Glu 
            420                 425                 430         


Leu Asp Glu Lys Val Asp Asp Leu Arg Ala Asp Thr Ile Ser Ser Gln 
        435                 440                 445             


Ile Glu Leu Ala Val Leu Leu Ser Asn Glu Gly Ile Ile Asn Ser Glu 
    450                 455                 460                 


Asp Glu His Leu Leu Ala Leu Glu Arg Lys Leu Lys Lys Met Leu Gly 
465                 470                 475                 480 


Pro Ser Ala Val Asp Ile Gly Asn Gly Cys Phe Glu Thr Lys His Lys 
                485                 490                 495     


Cys Asn Gln Thr Cys Leu Asp Arg Ile Ala Ala Gly Thr Phe Asn Ala 
            500                 505                 510         


Gly Glu Phe Ser Leu Pro Thr Phe Asp Ser Leu Asn Ile Thr Ala Ala 
        515                 520                 525             


Ser Leu Asn Asp Asp Gly Leu Asp Asn Tyr Gln Ile Leu Ala Ile Tyr 
    530                 535                 540                 


Ser Thr Val Ala Ser Ser Leu Val Leu Val Val Ser Leu Gly Ala Ile 
545                 550                 555                 560 


Ser Phe Trp Met Cys Ser Asn Gly Ser Leu Gln Cys Arg Ile Cys Ile 
                565                 570                 575     


<210>  133
<211>  1731
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PDI-B Sing-L203A DNA

<400>  133
atggcgaaaa acgttgcgat tttcggctta ttgttttctc ttcttgtgtt ggttccttct       60

cagatcttcg cggatcgaat ctgcactggg ataacatctt caaactcacc tcatgtggtc      120

aaaacagcta ctcaagggga ggtcaatgtg actggcgtga taccactgac aacaacacca      180

acaaaatctt attttgcaaa tctcaaagga acaaggacca gagggaaact atgcccggac      240

tgtctcaact gtacagatct ggatgtggcc ttgggcaggc caatgtgtgt ggggaccaca      300

ccttctgcta aagcttcaat actccatgag gtcagacctg ttacatccgg gtgctttcct      360

ataatgcacg acagaacaaa aatcaggcaa ctacccaatc ttctcagagg atatgaaaag      420

atcaggttat caacccaaaa cgttatcgat gcagaaaaag caccaggagg accctacaga      480

cttggaacct caggatcttg ccctaacgct accagtaaaa tcggattttt tgcaacaatg      540

gcttgggctg tcccaaagga caactacaaa aatgcaacga acccacaaac agtggaagta      600

ccatacattt gtacagaagg ggaagaccaa attactgttt gggggttcca ttcggataac      660

aaaacccaaa tgaagagcgc ctatggagac tcaaatcctc aaaagttcac ctcatctgct      720

aatggagtaa ccacgcatta tgtttctcag attggcgact tcccagatca aacagaagac      780

ggaggactac cacaaagcgg cagaattgtt gttgattaca tggtgcaaaa acctgggaaa      840

acaggaacaa ttgtctatca aaggggtgtt ttgttgcctc aaaaggtgtg gtgcgcgagt      900

ggcaggagca aagtaataaa agggtcattg cctttaattg gtgaagcaga ttgccttcat      960

gaagaatacg gtggattaaa caaaagcaag ccttactaca caggaaaaca tgcaaaagcc     1020

ataggaaatt gcccaatatg ggtaaaaaca cctttgaagc ttgccaatgg aaccaaatat     1080

agacctcctg gtggaggatg ggaaggaatg attgcaggtt ggcacggata cacatctcac     1140

ggagcacatg gagtggcagt ggcggcagac cttaagagta cacaagaagc tataaataag     1200

ataacaaaaa atctcaattc tttgagtgaa ctagaagtaa agaaccttca aagactaagt     1260

ggtgccatgg atgaactcca caacgaaata ctcgagctgg atgaaaaagt ggatgatctc     1320

agagctgaca ctataagctc acaaatagaa cttgcagtct tgctttccaa cgaaggaata     1380

ataaacagtg aagacgagca tctattggca cttgagagaa aactaaagaa aatgctgggt     1440

ccctctgctg tagacatagg aaacggatgc ttcgaaacca aacacaaatg caaccagacc     1500

tgcttagaca ggatagctgc tggcaccttt aatgcaggag aattttctct ccccactttt     1560

gattcattga acattactgc tgcatcttta aatgatgatg gattggataa ctaccagatt     1620

ttggcgatct attcaactgt cgccagttca ttggtactgg tagtctccct gggggcaatc     1680

agtttctgga tgtgctctaa tgggtctcta cagtgtagaa tatgtattta a              1731


<210>  134
<211>  576
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  PDI-B Sing-L203A AA

<400>  134

Met Ala Lys Asn Val Ala Ile Phe Gly Leu Leu Phe Ser Leu Leu Val 
1               5                   10                  15      


Leu Val Pro Ser Gln Ile Phe Ala Asp Arg Ile Cys Thr Gly Ile Thr 
            20                  25                  30          


Ser Ser Asn Ser Pro His Val Val Lys Thr Ala Thr Gln Gly Glu Val 
        35                  40                  45              


Asn Val Thr Gly Val Ile Pro Leu Thr Thr Thr Pro Thr Lys Ser Tyr 
    50                  55                  60                  


Phe Ala Asn Leu Lys Gly Thr Arg Thr Arg Gly Lys Leu Cys Pro Asp 
65                  70                  75                  80  


Cys Leu Asn Cys Thr Asp Leu Asp Val Ala Leu Gly Arg Pro Met Cys 
                85                  90                  95      


Val Gly Thr Thr Pro Ser Ala Lys Ala Ser Ile Leu His Glu Val Arg 
            100                 105                 110         


Pro Val Thr Ser Gly Cys Phe Pro Ile Met His Asp Arg Thr Lys Ile 
        115                 120                 125             


Arg Gln Leu Pro Asn Leu Leu Arg Gly Tyr Glu Lys Ile Arg Leu Ser 
    130                 135                 140                 


Thr Gln Asn Val Ile Asp Ala Glu Lys Ala Pro Gly Gly Pro Tyr Arg 
145                 150                 155                 160 


Leu Gly Thr Ser Gly Ser Cys Pro Asn Ala Thr Ser Lys Ile Gly Phe 
                165                 170                 175     


Phe Ala Thr Met Ala Trp Ala Val Pro Lys Asp Asn Tyr Lys Asn Ala 
            180                 185                 190         


Thr Asn Pro Gln Thr Val Glu Val Pro Tyr Ile Cys Thr Glu Gly Glu 
        195                 200                 205             


Asp Gln Ile Thr Val Trp Gly Phe His Ser Asp Asn Lys Thr Gln Met 
    210                 215                 220                 


Lys Ser Ala Tyr Gly Asp Ser Asn Pro Gln Lys Phe Thr Ser Ser Ala 
225                 230                 235                 240 


Asn Gly Val Thr Thr His Tyr Val Ser Gln Ile Gly Asp Phe Pro Asp 
                245                 250                 255     


Gln Thr Glu Asp Gly Gly Leu Pro Gln Ser Gly Arg Ile Val Val Asp 
            260                 265                 270         


Tyr Met Val Gln Lys Pro Gly Lys Thr Gly Thr Ile Val Tyr Gln Arg 
        275                 280                 285             


Gly Val Leu Leu Pro Gln Lys Val Trp Cys Ala Ser Gly Arg Ser Lys 
    290                 295                 300                 


Val Ile Lys Gly Ser Leu Pro Leu Ile Gly Glu Ala Asp Cys Leu His 
305                 310                 315                 320 


Glu Glu Tyr Gly Gly Leu Asn Lys Ser Lys Pro Tyr Tyr Thr Gly Lys 
                325                 330                 335     


His Ala Lys Ala Ile Gly Asn Cys Pro Ile Trp Val Lys Thr Pro Leu 
            340                 345                 350         


Lys Leu Ala Asn Gly Thr Lys Tyr Arg Pro Pro Gly Gly Gly Trp Glu 
        355                 360                 365             


Gly Met Ile Ala Gly Trp His Gly Tyr Thr Ser His Gly Ala His Gly 
    370                 375                 380                 


Val Ala Val Ala Ala Asp Leu Lys Ser Thr Gln Glu Ala Ile Asn Lys 
385                 390                 395                 400 


Ile Thr Lys Asn Leu Asn Ser Leu Ser Glu Leu Glu Val Lys Asn Leu 
                405                 410                 415     


Gln Arg Leu Ser Gly Ala Met Asp Glu Leu His Asn Glu Ile Leu Glu 
            420                 425                 430         


Leu Asp Glu Lys Val Asp Asp Leu Arg Ala Asp Thr Ile Ser Ser Gln 
        435                 440                 445             


Ile Glu Leu Ala Val Leu Leu Ser Asn Glu Gly Ile Ile Asn Ser Glu 
    450                 455                 460                 


Asp Glu His Leu Leu Ala Leu Glu Arg Lys Leu Lys Lys Met Leu Gly 
465                 470                 475                 480 


Pro Ser Ala Val Asp Ile Gly Asn Gly Cys Phe Glu Thr Lys His Lys 
                485                 490                 495     


Cys Asn Gln Thr Cys Leu Asp Arg Ile Ala Ala Gly Thr Phe Asn Ala 
            500                 505                 510         


Gly Glu Phe Ser Leu Pro Thr Phe Asp Ser Leu Asn Ile Thr Ala Ala 
        515                 520                 525             


Ser Leu Asn Asp Asp Gly Leu Asp Asn Tyr Gln Ile Leu Ala Ile Tyr 
    530                 535                 540                 


Ser Thr Val Ala Ser Ser Leu Val Leu Val Val Ser Leu Gly Ala Ile 
545                 550                 555                 560 


Ser Phe Trp Met Cys Ser Asn Gly Ser Leu Gln Cys Arg Ile Cys Ile 
                565                 570                 575     


<210>  135
<211>  1731
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PDI-B Sing-L203W DNA

<400>  135
atggcgaaaa acgttgcgat tttcggctta ttgttttctc ttcttgtgtt ggttccttct       60

cagatcttcg cggatcgaat ctgcactggg ataacatctt caaactcacc tcatgtggtc      120

aaaacagcta ctcaagggga ggtcaatgtg actggcgtga taccactgac aacaacacca      180

acaaaatctt attttgcaaa tctcaaagga acaaggacca gagggaaact atgcccggac      240

tgtctcaact gtacagatct ggatgtggcc ttgggcaggc caatgtgtgt ggggaccaca      300

ccttctgcta aagcttcaat actccatgag gtcagacctg ttacatccgg gtgctttcct      360

ataatgcacg acagaacaaa aatcaggcaa ctacccaatc ttctcagagg atatgaaaag      420

atcaggttat caacccaaaa cgttatcgat gcagaaaaag caccaggagg accctacaga      480

cttggaacct caggatcttg ccctaacgct accagtaaaa tcggattttt tgcaacaatg      540

gcttgggctg tcccaaagga caactacaaa aatgcaacga acccacaaac agtggaagta      600

ccatacattt gtacagaagg ggaagaccaa attactgttt gggggttcca ttcggataac      660

aaaacccaaa tgaagagctg gtatggagac tcaaatcctc aaaagttcac ctcatctgct      720

aatggagtaa ccacgcatta tgtttctcag attggcgact tcccagatca aacagaagac      780

ggaggactac cacaaagcgg cagaattgtt gttgattaca tggtgcaaaa acctgggaaa      840

acaggaacaa ttgtctatca aaggggtgtt ttgttgcctc aaaaggtgtg gtgcgcgagt      900

ggcaggagca aagtaataaa agggtcattg cctttaattg gtgaagcaga ttgccttcat      960

gaagaatacg gtggattaaa caaaagcaag ccttactaca caggaaaaca tgcaaaagcc     1020

ataggaaatt gcccaatatg ggtaaaaaca cctttgaagc ttgccaatgg aaccaaatat     1080

agacctcctg gtggaggatg ggaaggaatg attgcaggtt ggcacggata cacatctcac     1140

ggagcacatg gagtggcagt ggcggcagac cttaagagta cacaagaagc tataaataag     1200

ataacaaaaa atctcaattc tttgagtgaa ctagaagtaa agaaccttca aagactaagt     1260

ggtgccatgg atgaactcca caacgaaata ctcgagctgg atgaaaaagt ggatgatctc     1320

agagctgaca ctataagctc acaaatagaa cttgcagtct tgctttccaa cgaaggaata     1380

ataaacagtg aagacgagca tctattggca cttgagagaa aactaaagaa aatgctgggt     1440

ccctctgctg tagacatagg aaacggatgc ttcgaaacca aacacaaatg caaccagacc     1500

tgcttagaca ggatagctgc tggcaccttt aatgcaggag aattttctct ccccactttt     1560

gattcattga acattactgc tgcatcttta aatgatgatg gattggataa ctaccagatt     1620

ttggcgatct attcaactgt cgccagttca ttggtactgg tagtctccct gggggcaatc     1680

agtttctgga tgtgctctaa tgggtctcta cagtgtagaa tatgtattta a              1731


<210>  136
<211>  576
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  PDI-B Sing-L203W AA

<400>  136

Met Ala Lys Asn Val Ala Ile Phe Gly Leu Leu Phe Ser Leu Leu Val 
1               5                   10                  15      


Leu Val Pro Ser Gln Ile Phe Ala Asp Arg Ile Cys Thr Gly Ile Thr 
            20                  25                  30          


Ser Ser Asn Ser Pro His Val Val Lys Thr Ala Thr Gln Gly Glu Val 
        35                  40                  45              


Asn Val Thr Gly Val Ile Pro Leu Thr Thr Thr Pro Thr Lys Ser Tyr 
    50                  55                  60                  


Phe Ala Asn Leu Lys Gly Thr Arg Thr Arg Gly Lys Leu Cys Pro Asp 
65                  70                  75                  80  


Cys Leu Asn Cys Thr Asp Leu Asp Val Ala Leu Gly Arg Pro Met Cys 
                85                  90                  95      


Val Gly Thr Thr Pro Ser Ala Lys Ala Ser Ile Leu His Glu Val Arg 
            100                 105                 110         


Pro Val Thr Ser Gly Cys Phe Pro Ile Met His Asp Arg Thr Lys Ile 
        115                 120                 125             


Arg Gln Leu Pro Asn Leu Leu Arg Gly Tyr Glu Lys Ile Arg Leu Ser 
    130                 135                 140                 


Thr Gln Asn Val Ile Asp Ala Glu Lys Ala Pro Gly Gly Pro Tyr Arg 
145                 150                 155                 160 


Leu Gly Thr Ser Gly Ser Cys Pro Asn Ala Thr Ser Lys Ile Gly Phe 
                165                 170                 175     


Phe Ala Thr Met Ala Trp Ala Val Pro Lys Asp Asn Tyr Lys Asn Ala 
            180                 185                 190         


Thr Asn Pro Gln Thr Val Glu Val Pro Tyr Ile Cys Thr Glu Gly Glu 
        195                 200                 205             


Asp Gln Ile Thr Val Trp Gly Phe His Ser Asp Asn Lys Thr Gln Met 
    210                 215                 220                 


Lys Ser Trp Tyr Gly Asp Ser Asn Pro Gln Lys Phe Thr Ser Ser Ala 
225                 230                 235                 240 


Asn Gly Val Thr Thr His Tyr Val Ser Gln Ile Gly Asp Phe Pro Asp 
                245                 250                 255     


Gln Thr Glu Asp Gly Gly Leu Pro Gln Ser Gly Arg Ile Val Val Asp 
            260                 265                 270         


Tyr Met Val Gln Lys Pro Gly Lys Thr Gly Thr Ile Val Tyr Gln Arg 
        275                 280                 285             


Gly Val Leu Leu Pro Gln Lys Val Trp Cys Ala Ser Gly Arg Ser Lys 
    290                 295                 300                 


Val Ile Lys Gly Ser Leu Pro Leu Ile Gly Glu Ala Asp Cys Leu His 
305                 310                 315                 320 


Glu Glu Tyr Gly Gly Leu Asn Lys Ser Lys Pro Tyr Tyr Thr Gly Lys 
                325                 330                 335     


His Ala Lys Ala Ile Gly Asn Cys Pro Ile Trp Val Lys Thr Pro Leu 
            340                 345                 350         


Lys Leu Ala Asn Gly Thr Lys Tyr Arg Pro Pro Gly Gly Gly Trp Glu 
        355                 360                 365             


Gly Met Ile Ala Gly Trp His Gly Tyr Thr Ser His Gly Ala His Gly 
    370                 375                 380                 


Val Ala Val Ala Ala Asp Leu Lys Ser Thr Gln Glu Ala Ile Asn Lys 
385                 390                 395                 400 


Ile Thr Lys Asn Leu Asn Ser Leu Ser Glu Leu Glu Val Lys Asn Leu 
                405                 410                 415     


Gln Arg Leu Ser Gly Ala Met Asp Glu Leu His Asn Glu Ile Leu Glu 
            420                 425                 430         


Leu Asp Glu Lys Val Asp Asp Leu Arg Ala Asp Thr Ile Ser Ser Gln 
        435                 440                 445             


Ile Glu Leu Ala Val Leu Leu Ser Asn Glu Gly Ile Ile Asn Ser Glu 
    450                 455                 460                 


Asp Glu His Leu Leu Ala Leu Glu Arg Lys Leu Lys Lys Met Leu Gly 
465                 470                 475                 480 


Pro Ser Ala Val Asp Ile Gly Asn Gly Cys Phe Glu Thr Lys His Lys 
                485                 490                 495     


Cys Asn Gln Thr Cys Leu Asp Arg Ile Ala Ala Gly Thr Phe Asn Ala 
            500                 505                 510         


Gly Glu Phe Ser Leu Pro Thr Phe Asp Ser Leu Asn Ile Thr Ala Ala 
        515                 520                 525             


Ser Leu Asn Asp Asp Gly Leu Asp Asn Tyr Gln Ile Leu Ala Ile Tyr 
    530                 535                 540                 


Ser Thr Val Ala Ser Ser Leu Val Leu Val Val Ser Leu Gly Ala Ile 
545                 550                 555                 560 


Ser Phe Trp Met Cys Ser Asn Gly Ser Leu Gln Cys Arg Ile Cys Ile 
                565                 570                 575     


<210>  137
<211>  1728
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PDI-B Mary DNA

<400>  137
atggcgaaaa acgttgcgat tttcggctta ttgttttctc ttcttgtgtt ggttccttct       60

cagatcttcg cggatcgaat ctgcactggg ataacatcgt caaactcacc acatgtcgtc      120

aaaactgcta ctcaagggga ggtcaatgtg accggtgtaa taccactgac aacaacaccc      180

accaaatctc attttgcaaa tctcaaagga acagaaacca gggggaaact atgcccaaaa      240

tgcctcaact gcacagatct ggatgtagcc ttgggcagac caaaatgcac agggaaaata      300

ccctctgcaa gggtttcaat actccatgaa gtcagacctg ttacatctgg gtgctttcct      360

ataatgcacg atagaacaaa aattagacag ctgcctaacc ttctccgagg atacgaacat      420

gtcaggttat caactcacaa cgttatcaat gcagaagacg caccaggagg accctacaaa      480

attggaacct cagggtcttg ccctaacatt accaatggaa acggattctt cgcaacaatg      540

gcttgggccg tcccagacaa aaacaaaaca gcaacaaatc cattaacaat agaagtacca      600

tacgtgtgta cagaaggaga agaccaaatt accgtttggg ggttccactc tgacaacgag      660

acccaaatgg caaagctcta tggggactca aagccccaga agttcacctc atctgccaac      720

ggagtgacca cacattacgt ttcacagatt ggtggcttcc caaatcaaac agaagacgga      780

ggactaccac aaagtggcag aattgttgtt gattacatgg tgcaaaaatc tggaaaaaca      840

ggaacaatta cctatcaaag aggtatttta ttgcctcaaa aggtgtggtg cgcaagtggc      900

aggagcaagg taataaaagg atccttgcct ttaattggag aagcagattg cctccatgaa      960

aaatacggtg gattaaacaa aagcaagcct tactacacag gggaacatgc aaaggccata     1020

ggaaattgcc caatatgggt gaaaacaccc ttgaagctgg ccaatggaac caaatataga     1080

cctcctggtg gaggatggga aggaatgatt gcaggttggc acggatacac atcccatggg     1140

gcacatggag tagcggtggc agcagacctt aagagcactc aagaggccat aaacaagata     1200

acaaaaaatc tcaactcttt gagtgagctg gaagtaaaga atcttcaaag actaagcggt     1260

gccatggatg aactccacaa cgaaatacta gaactagatg agaaagtgga tgatctcaga     1320

gctgatacaa taagctcaca aatagaactc gcagtcctgc tttccaatga aggaataata     1380

aacagtgaag atgaacatct cttggcgctt gaaagaaagc tgaagaaaat gctgggcccc     1440

tctgctgtag agatagggaa tggatgcttt gaaaccaaac acaagtgcaa ccagacctgt     1500

ctcgacaaga tagctgctgg tacctttgat gcaggagaat tttctctccc cacctttgat     1560

tcactgaata ttactgctgc atctttaaat gacgatggat tggataatta ccagattttg     1620

gcgatctatt caactgtcgc cagttcattg gtactggtag tctccctggg ggcaatcagt     1680

ttctggatgt gctctaatgg gtctctacag tgtagaatat gtatttaa                  1728


<210>  138
<211>  575
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  PDI-B Mary AA

<400>  138

Met Ala Lys Asn Val Ala Ile Phe Gly Leu Leu Phe Ser Leu Leu Val 
1               5                   10                  15      


Leu Val Pro Ser Gln Ile Phe Ala Asp Arg Ile Cys Thr Gly Ile Thr 
            20                  25                  30          


Ser Ser Asn Ser Pro His Val Val Lys Thr Ala Thr Gln Gly Glu Val 
        35                  40                  45              


Asn Val Thr Gly Val Ile Pro Leu Thr Thr Thr Pro Thr Lys Ser His 
    50                  55                  60                  


Phe Ala Asn Leu Lys Gly Thr Glu Thr Arg Gly Lys Leu Cys Pro Lys 
65                  70                  75                  80  


Cys Leu Asn Cys Thr Asp Leu Asp Val Ala Leu Gly Arg Pro Lys Cys 
                85                  90                  95      


Thr Gly Lys Ile Pro Ser Ala Arg Val Ser Ile Leu His Glu Val Arg 
            100                 105                 110         


Pro Val Thr Ser Gly Cys Phe Pro Ile Met His Asp Arg Thr Lys Ile 
        115                 120                 125             


Arg Gln Leu Pro Asn Leu Leu Arg Gly Tyr Glu His Val Arg Leu Ser 
    130                 135                 140                 


Thr His Asn Val Ile Asn Ala Glu Asp Ala Pro Gly Gly Pro Tyr Lys 
145                 150                 155                 160 


Ile Gly Thr Ser Gly Ser Cys Pro Asn Ile Thr Asn Gly Asn Gly Phe 
                165                 170                 175     


Phe Ala Thr Met Ala Trp Ala Val Pro Asp Lys Asn Lys Thr Ala Thr 
            180                 185                 190         


Asn Pro Leu Thr Ile Glu Val Pro Tyr Val Cys Thr Glu Gly Glu Asp 
        195                 200                 205             


Gln Ile Thr Val Trp Gly Phe His Ser Asp Asn Glu Thr Gln Met Ala 
    210                 215                 220                 


Lys Leu Tyr Gly Asp Ser Lys Pro Gln Lys Phe Thr Ser Ser Ala Asn 
225                 230                 235                 240 


Gly Val Thr Thr His Tyr Val Ser Gln Ile Gly Gly Phe Pro Asn Gln 
                245                 250                 255     


Thr Glu Asp Gly Gly Leu Pro Gln Ser Gly Arg Ile Val Val Asp Tyr 
            260                 265                 270         


Met Val Gln Lys Ser Gly Lys Thr Gly Thr Ile Thr Tyr Gln Arg Gly 
        275                 280                 285             


Ile Leu Leu Pro Gln Lys Val Trp Cys Ala Ser Gly Arg Ser Lys Val 
    290                 295                 300                 


Ile Lys Gly Ser Leu Pro Leu Ile Gly Glu Ala Asp Cys Leu His Glu 
305                 310                 315                 320 


Lys Tyr Gly Gly Leu Asn Lys Ser Lys Pro Tyr Tyr Thr Gly Glu His 
                325                 330                 335     


Ala Lys Ala Ile Gly Asn Cys Pro Ile Trp Val Lys Thr Pro Leu Lys 
            340                 345                 350         


Leu Ala Asn Gly Thr Lys Tyr Arg Pro Pro Gly Gly Gly Trp Glu Gly 
        355                 360                 365             


Met Ile Ala Gly Trp His Gly Tyr Thr Ser His Gly Ala His Gly Val 
    370                 375                 380                 


Ala Val Ala Ala Asp Leu Lys Ser Thr Gln Glu Ala Ile Asn Lys Ile 
385                 390                 395                 400 


Thr Lys Asn Leu Asn Ser Leu Ser Glu Leu Glu Val Lys Asn Leu Gln 
                405                 410                 415     


Arg Leu Ser Gly Ala Met Asp Glu Leu His Asn Glu Ile Leu Glu Leu 
            420                 425                 430         


Asp Glu Lys Val Asp Asp Leu Arg Ala Asp Thr Ile Ser Ser Gln Ile 
        435                 440                 445             


Glu Leu Ala Val Leu Leu Ser Asn Glu Gly Ile Ile Asn Ser Glu Asp 
    450                 455                 460                 


Glu His Leu Leu Ala Leu Glu Arg Lys Leu Lys Lys Met Leu Gly Pro 
465                 470                 475                 480 


Ser Ala Val Glu Ile Gly Asn Gly Cys Phe Glu Thr Lys His Lys Cys 
                485                 490                 495     


Asn Gln Thr Cys Leu Asp Lys Ile Ala Ala Gly Thr Phe Asp Ala Gly 
            500                 505                 510         


Glu Phe Ser Leu Pro Thr Phe Asp Ser Leu Asn Ile Thr Ala Ala Ser 
        515                 520                 525             


Leu Asn Asp Asp Gly Leu Asp Asn Tyr Gln Ile Leu Ala Ile Tyr Ser 
    530                 535                 540                 


Thr Val Ala Ser Ser Leu Val Leu Val Val Ser Leu Gly Ala Ile Ser 
545                 550                 555                 560 


Phe Trp Met Cys Ser Asn Gly Ser Leu Gln Cys Arg Ile Cys Ile 
                565                 570                 575 


<210>  139
<211>  50
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  IF-B-Bris(nat).c

<400>  139
tctcagatct tcgcggatcg aatctgcact gggataacat cgtcaaactc                  50


<210>  140
<211>  1728
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PDI-B Mary-G138A DNA

<400>  140
atggcgaaaa acgttgcgat tttcggctta ttgttttctc ttcttgtgtt ggttccttct       60

cagatcttcg cggatcgaat ctgcactggg ataacatcgt caaactcacc acatgtcgtc      120

aaaactgcta ctcaagggga ggtcaatgtg accggtgtaa taccactgac aacaacaccc      180

accaaatctc attttgcaaa tctcaaagga acagaaacca gggggaaact atgcccaaaa      240

tgcctcaact gcacagatct ggatgtagcc ttgggcagac caaaatgcac agggaaaata      300

ccctctgcaa gggtttcaat actccatgaa gtcagacctg ttacatctgg gtgctttcct      360

ataatgcacg atagaacaaa aattagacag ctgcctaacc ttctccgagg atacgaacat      420

gtcaggttat caactcacaa cgttatcaat gcagaagacg caccaggagg accctacaaa      480

attgccacct cagggtcttg ccctaacatt accaatggaa acggattctt cgcaacaatg      540

gcttgggccg tcccagacaa aaacaaaaca gcaacaaatc cattaacaat agaagtacca      600

tacgtgtgta cagaaggaga agaccaaatt accgtttggg ggttccactc tgacaacgag      660

acccaaatgg caaagctcta tggggactca aagccccaga agttcacctc atctgccaac      720

ggagtgacca cacattacgt ttcacagatt ggtggcttcc caaatcaaac agaagacgga      780

ggactaccac aaagtggcag aattgttgtt gattacatgg tgcaaaaatc tggaaaaaca      840

ggaacaatta cctatcaaag aggtatttta ttgcctcaaa aggtgtggtg cgcaagtggc      900

aggagcaagg taataaaagg atccttgcct ttaattggag aagcagattg cctccatgaa      960

aaatacggtg gattaaacaa aagcaagcct tactacacag gggaacatgc aaaggccata     1020

ggaaattgcc caatatgggt gaaaacaccc ttgaagctgg ccaatggaac caaatataga     1080

cctcctggtg gaggatggga aggaatgatt gcaggttggc acggatacac atcccatggg     1140

gcacatggag tagcggtggc agcagacctt aagagcactc aagaggccat aaacaagata     1200

acaaaaaatc tcaactcttt gagtgagctg gaagtaaaga atcttcaaag actaagcggt     1260

gccatggatg aactccacaa cgaaatacta gaactagatg agaaagtgga tgatctcaga     1320

gctgatacaa taagctcaca aatagaactc gcagtcctgc tttccaatga aggaataata     1380

aacagtgaag atgaacatct cttggcgctt gaaagaaagc tgaagaaaat gctgggcccc     1440

tctgctgtag agatagggaa tggatgcttt gaaaccaaac acaagtgcaa ccagacctgt     1500

ctcgacaaga tagctgctgg tacctttgat gcaggagaat tttctctccc cacctttgat     1560

tcactgaata ttactgctgc atctttaaat gacgatggat tggataatta ccagattttg     1620

gcgatctatt caactgtcgc cagttcattg gtactggtag tctccctggg ggcaatcagt     1680

ttctggatgt gctctaatgg gtctctacag tgtagaatat gtatttaa                  1728


<210>  141
<211>  575
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  PDI-B Mary-G138A AA

<400>  141

Met Ala Lys Asn Val Ala Ile Phe Gly Leu Leu Phe Ser Leu Leu Val 
1               5                   10                  15      


Leu Val Pro Ser Gln Ile Phe Ala Asp Arg Ile Cys Thr Gly Ile Thr 
            20                  25                  30          


Ser Ser Asn Ser Pro His Val Val Lys Thr Ala Thr Gln Gly Glu Val 
        35                  40                  45              


Asn Val Thr Gly Val Ile Pro Leu Thr Thr Thr Pro Thr Lys Ser His 
    50                  55                  60                  


Phe Ala Asn Leu Lys Gly Thr Glu Thr Arg Gly Lys Leu Cys Pro Lys 
65                  70                  75                  80  


Cys Leu Asn Cys Thr Asp Leu Asp Val Ala Leu Gly Arg Pro Lys Cys 
                85                  90                  95      


Thr Gly Lys Ile Pro Ser Ala Arg Val Ser Ile Leu His Glu Val Arg 
            100                 105                 110         


Pro Val Thr Ser Gly Cys Phe Pro Ile Met His Asp Arg Thr Lys Ile 
        115                 120                 125             


Arg Gln Leu Pro Asn Leu Leu Arg Gly Tyr Glu His Val Arg Leu Ser 
    130                 135                 140                 


Thr His Asn Val Ile Asn Ala Glu Asp Ala Pro Gly Gly Pro Tyr Lys 
145                 150                 155                 160 


Ile Ala Thr Ser Gly Ser Cys Pro Asn Ile Thr Asn Gly Asn Gly Phe 
                165                 170                 175     


Phe Ala Thr Met Ala Trp Ala Val Pro Asp Lys Asn Lys Thr Ala Thr 
            180                 185                 190         


Asn Pro Leu Thr Ile Glu Val Pro Tyr Val Cys Thr Glu Gly Glu Asp 
        195                 200                 205             


Gln Ile Thr Val Trp Gly Phe His Ser Asp Asn Glu Thr Gln Met Ala 
    210                 215                 220                 


Lys Leu Tyr Gly Asp Ser Lys Pro Gln Lys Phe Thr Ser Ser Ala Asn 
225                 230                 235                 240 


Gly Val Thr Thr His Tyr Val Ser Gln Ile Gly Gly Phe Pro Asn Gln 
                245                 250                 255     


Thr Glu Asp Gly Gly Leu Pro Gln Ser Gly Arg Ile Val Val Asp Tyr 
            260                 265                 270         


Met Val Gln Lys Ser Gly Lys Thr Gly Thr Ile Thr Tyr Gln Arg Gly 
        275                 280                 285             


Ile Leu Leu Pro Gln Lys Val Trp Cys Ala Ser Gly Arg Ser Lys Val 
    290                 295                 300                 


Ile Lys Gly Ser Leu Pro Leu Ile Gly Glu Ala Asp Cys Leu His Glu 
305                 310                 315                 320 


Lys Tyr Gly Gly Leu Asn Lys Ser Lys Pro Tyr Tyr Thr Gly Glu His 
                325                 330                 335     


Ala Lys Ala Ile Gly Asn Cys Pro Ile Trp Val Lys Thr Pro Leu Lys 
            340                 345                 350         


Leu Ala Asn Gly Thr Lys Tyr Arg Pro Pro Gly Gly Gly Trp Glu Gly 
        355                 360                 365             


Met Ile Ala Gly Trp His Gly Tyr Thr Ser His Gly Ala His Gly Val 
    370                 375                 380                 


Ala Val Ala Ala Asp Leu Lys Ser Thr Gln Glu Ala Ile Asn Lys Ile 
385                 390                 395                 400 


Thr Lys Asn Leu Asn Ser Leu Ser Glu Leu Glu Val Lys Asn Leu Gln 
                405                 410                 415     


Arg Leu Ser Gly Ala Met Asp Glu Leu His Asn Glu Ile Leu Glu Leu 
            420                 425                 430         


Asp Glu Lys Val Asp Asp Leu Arg Ala Asp Thr Ile Ser Ser Gln Ile 
        435                 440                 445             


Glu Leu Ala Val Leu Leu Ser Asn Glu Gly Ile Ile Asn Ser Glu Asp 
    450                 455                 460                 


Glu His Leu Leu Ala Leu Glu Arg Lys Leu Lys Lys Met Leu Gly Pro 
465                 470                 475                 480 


Ser Ala Val Glu Ile Gly Asn Gly Cys Phe Glu Thr Lys His Lys Cys 
                485                 490                 495     


Asn Gln Thr Cys Leu Asp Lys Ile Ala Ala Gly Thr Phe Asp Ala Gly 
            500                 505                 510         


Glu Phe Ser Leu Pro Thr Phe Asp Ser Leu Asn Ile Thr Ala Ala Ser 
        515                 520                 525             


Leu Asn Asp Asp Gly Leu Asp Asn Tyr Gln Ile Leu Ala Ile Tyr Ser 
    530                 535                 540                 


Thr Val Ala Ser Ser Leu Val Leu Val Val Ser Leu Gly Ala Ile Ser 
545                 550                 555                 560 


Phe Trp Met Cys Ser Asn Gly Ser Leu Gln Cys Arg Ile Cys Ile 
                565                 570                 575 


<210>  142
<211>  1728
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PDI-B Mary-S140A DNA

<400>  142
atggcgaaaa acgttgcgat tttcggctta ttgttttctc ttcttgtgtt ggttccttct       60

cagatcttcg cggatcgaat ctgcactggg ataacatcgt caaactcacc acatgtcgtc      120

aaaactgcta ctcaagggga ggtcaatgtg accggtgtaa taccactgac aacaacaccc      180

accaaatctc attttgcaaa tctcaaagga acagaaacca gggggaaact atgcccaaaa      240

tgcctcaact gcacagatct ggatgtagcc ttgggcagac caaaatgcac agggaaaata      300

ccctctgcaa gggtttcaat actccatgaa gtcagacctg ttacatctgg gtgctttcct      360

ataatgcacg atagaacaaa aattagacag ctgcctaacc ttctccgagg atacgaacat      420

gtcaggttat caactcacaa cgttatcaat gcagaagacg caccaggagg accctacaaa      480

attggaaccg ccgggtcttg ccctaacatt accaatggaa acggattctt cgcaacaatg      540

gcttgggccg tcccagacaa aaacaaaaca gcaacaaatc cattaacaat agaagtacca      600

tacgtgtgta cagaaggaga agaccaaatt accgtttggg ggttccactc tgacaacgag      660

acccaaatgg caaagctcta tggggactca aagccccaga agttcacctc atctgccaac      720

ggagtgacca cacattacgt ttcacagatt ggtggcttcc caaatcaaac agaagacgga      780

ggactaccac aaagtggcag aattgttgtt gattacatgg tgcaaaaatc tggaaaaaca      840

ggaacaatta cctatcaaag aggtatttta ttgcctcaaa aggtgtggtg cgcaagtggc      900

aggagcaagg taataaaagg atccttgcct ttaattggag aagcagattg cctccatgaa      960

aaatacggtg gattaaacaa aagcaagcct tactacacag gggaacatgc aaaggccata     1020

ggaaattgcc caatatgggt gaaaacaccc ttgaagctgg ccaatggaac caaatataga     1080

cctcctggtg gaggatggga aggaatgatt gcaggttggc acggatacac atcccatggg     1140

gcacatggag tagcggtggc agcagacctt aagagcactc aagaggccat aaacaagata     1200

acaaaaaatc tcaactcttt gagtgagctg gaagtaaaga atcttcaaag actaagcggt     1260

gccatggatg aactccacaa cgaaatacta gaactagatg agaaagtgga tgatctcaga     1320

gctgatacaa taagctcaca aatagaactc gcagtcctgc tttccaatga aggaataata     1380

aacagtgaag atgaacatct cttggcgctt gaaagaaagc tgaagaaaat gctgggcccc     1440

tctgctgtag agatagggaa tggatgcttt gaaaccaaac acaagtgcaa ccagacctgt     1500

ctcgacaaga tagctgctgg tacctttgat gcaggagaat tttctctccc cacctttgat     1560

tcactgaata ttactgctgc atctttaaat gacgatggat tggataatta ccagattttg     1620

gcgatctatt caactgtcgc cagttcattg gtactggtag tctccctggg ggcaatcagt     1680

ttctggatgt gctctaatgg gtctctacag tgtagaatat gtatttaa                  1728


<210>  143
<211>  575
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  PDI-B Mary-S140A AA

<400>  143

Met Ala Lys Asn Val Ala Ile Phe Gly Leu Leu Phe Ser Leu Leu Val 
1               5                   10                  15      


Leu Val Pro Ser Gln Ile Phe Ala Asp Arg Ile Cys Thr Gly Ile Thr 
            20                  25                  30          


Ser Ser Asn Ser Pro His Val Val Lys Thr Ala Thr Gln Gly Glu Val 
        35                  40                  45              


Asn Val Thr Gly Val Ile Pro Leu Thr Thr Thr Pro Thr Lys Ser His 
    50                  55                  60                  


Phe Ala Asn Leu Lys Gly Thr Glu Thr Arg Gly Lys Leu Cys Pro Lys 
65                  70                  75                  80  


Cys Leu Asn Cys Thr Asp Leu Asp Val Ala Leu Gly Arg Pro Lys Cys 
                85                  90                  95      


Thr Gly Lys Ile Pro Ser Ala Arg Val Ser Ile Leu His Glu Val Arg 
            100                 105                 110         


Pro Val Thr Ser Gly Cys Phe Pro Ile Met His Asp Arg Thr Lys Ile 
        115                 120                 125             


Arg Gln Leu Pro Asn Leu Leu Arg Gly Tyr Glu His Val Arg Leu Ser 
    130                 135                 140                 


Thr His Asn Val Ile Asn Ala Glu Asp Ala Pro Gly Gly Pro Tyr Lys 
145                 150                 155                 160 


Ile Gly Thr Ala Gly Ser Cys Pro Asn Ile Thr Asn Gly Asn Gly Phe 
                165                 170                 175     


Phe Ala Thr Met Ala Trp Ala Val Pro Asp Lys Asn Lys Thr Ala Thr 
            180                 185                 190         


Asn Pro Leu Thr Ile Glu Val Pro Tyr Val Cys Thr Glu Gly Glu Asp 
        195                 200                 205             


Gln Ile Thr Val Trp Gly Phe His Ser Asp Asn Glu Thr Gln Met Ala 
    210                 215                 220                 


Lys Leu Tyr Gly Asp Ser Lys Pro Gln Lys Phe Thr Ser Ser Ala Asn 
225                 230                 235                 240 


Gly Val Thr Thr His Tyr Val Ser Gln Ile Gly Gly Phe Pro Asn Gln 
                245                 250                 255     


Thr Glu Asp Gly Gly Leu Pro Gln Ser Gly Arg Ile Val Val Asp Tyr 
            260                 265                 270         


Met Val Gln Lys Ser Gly Lys Thr Gly Thr Ile Thr Tyr Gln Arg Gly 
        275                 280                 285             


Ile Leu Leu Pro Gln Lys Val Trp Cys Ala Ser Gly Arg Ser Lys Val 
    290                 295                 300                 


Ile Lys Gly Ser Leu Pro Leu Ile Gly Glu Ala Asp Cys Leu His Glu 
305                 310                 315                 320 


Lys Tyr Gly Gly Leu Asn Lys Ser Lys Pro Tyr Tyr Thr Gly Glu His 
                325                 330                 335     


Ala Lys Ala Ile Gly Asn Cys Pro Ile Trp Val Lys Thr Pro Leu Lys 
            340                 345                 350         


Leu Ala Asn Gly Thr Lys Tyr Arg Pro Pro Gly Gly Gly Trp Glu Gly 
        355                 360                 365             


Met Ile Ala Gly Trp His Gly Tyr Thr Ser His Gly Ala His Gly Val 
    370                 375                 380                 


Ala Val Ala Ala Asp Leu Lys Ser Thr Gln Glu Ala Ile Asn Lys Ile 
385                 390                 395                 400 


Thr Lys Asn Leu Asn Ser Leu Ser Glu Leu Glu Val Lys Asn Leu Gln 
                405                 410                 415     


Arg Leu Ser Gly Ala Met Asp Glu Leu His Asn Glu Ile Leu Glu Leu 
            420                 425                 430         


Asp Glu Lys Val Asp Asp Leu Arg Ala Asp Thr Ile Ser Ser Gln Ile 
        435                 440                 445             


Glu Leu Ala Val Leu Leu Ser Asn Glu Gly Ile Ile Asn Ser Glu Asp 
    450                 455                 460                 


Glu His Leu Leu Ala Leu Glu Arg Lys Leu Lys Lys Met Leu Gly Pro 
465                 470                 475                 480 


Ser Ala Val Glu Ile Gly Asn Gly Cys Phe Glu Thr Lys His Lys Cys 
                485                 490                 495     


Asn Gln Thr Cys Leu Asp Lys Ile Ala Ala Gly Thr Phe Asp Ala Gly 
            500                 505                 510         


Glu Phe Ser Leu Pro Thr Phe Asp Ser Leu Asn Ile Thr Ala Ala Ser 
        515                 520                 525             


Leu Asn Asp Asp Gly Leu Asp Asn Tyr Gln Ile Leu Ala Ile Tyr Ser 
    530                 535                 540                 


Thr Val Ala Ser Ser Leu Val Leu Val Val Ser Leu Gly Ala Ile Ser 
545                 550                 555                 560 


Phe Trp Met Cys Ser Asn Gly Ser Leu Gln Cys Arg Ile Cys Ile 
                565                 570                 575 


<210>  144
<211>  1728
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PDI-B Mary-S142A DNA

<400>  144
atggcgaaaa acgttgcgat tttcggctta ttgttttctc ttcttgtgtt ggttccttct       60

cagatcttcg cggatcgaat ctgcactggg ataacatcgt caaactcacc acatgtcgtc      120

aaaactgcta ctcaagggga ggtcaatgtg accggtgtaa taccactgac aacaacaccc      180

accaaatctc attttgcaaa tctcaaagga acagaaacca gggggaaact atgcccaaaa      240

tgcctcaact gcacagatct ggatgtagcc ttgggcagac caaaatgcac agggaaaata      300

ccctctgcaa gggtttcaat actccatgaa gtcagacctg ttacatctgg gtgctttcct      360

ataatgcacg atagaacaaa aattagacag ctgcctaacc ttctccgagg atacgaacat      420

gtcaggttat caactcacaa cgttatcaat gcagaagacg caccaggagg accctacaaa      480

attggaacct caggggcctg ccctaacatt accaatggaa acggattctt cgcaacaatg      540

gcttgggccg tcccagacaa aaacaaaaca gcaacaaatc cattaacaat agaagtacca      600

tacgtgtgta cagaaggaga agaccaaatt accgtttggg ggttccactc tgacaacgag      660

acccaaatgg caaagctcta tggggactca aagccccaga agttcacctc atctgccaac      720

ggagtgacca cacattacgt ttcacagatt ggtggcttcc caaatcaaac agaagacgga      780

ggactaccac aaagtggcag aattgttgtt gattacatgg tgcaaaaatc tggaaaaaca      840

ggaacaatta cctatcaaag aggtatttta ttgcctcaaa aggtgtggtg cgcaagtggc      900

aggagcaagg taataaaagg atccttgcct ttaattggag aagcagattg cctccatgaa      960

aaatacggtg gattaaacaa aagcaagcct tactacacag gggaacatgc aaaggccata     1020

ggaaattgcc caatatgggt gaaaacaccc ttgaagctgg ccaatggaac caaatataga     1080

cctcctggtg gaggatggga aggaatgatt gcaggttggc acggatacac atcccatggg     1140

gcacatggag tagcggtggc agcagacctt aagagcactc aagaggccat aaacaagata     1200

acaaaaaatc tcaactcttt gagtgagctg gaagtaaaga atcttcaaag actaagcggt     1260

gccatggatg aactccacaa cgaaatacta gaactagatg agaaagtgga tgatctcaga     1320

gctgatacaa taagctcaca aatagaactc gcagtcctgc tttccaatga aggaataata     1380

aacagtgaag atgaacatct cttggcgctt gaaagaaagc tgaagaaaat gctgggcccc     1440

tctgctgtag agatagggaa tggatgcttt gaaaccaaac acaagtgcaa ccagacctgt     1500

ctcgacaaga tagctgctgg tacctttgat gcaggagaat tttctctccc cacctttgat     1560

tcactgaata ttactgctgc atctttaaat gacgatggat tggataatta ccagattttg     1620

gcgatctatt caactgtcgc cagttcattg gtactggtag tctccctggg ggcaatcagt     1680

ttctggatgt gctctaatgg gtctctacag tgtagaatat gtatttaa                  1728


<210>  145
<211>  575
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  PDI-B Mary-S142A AA

<400>  145

Met Ala Lys Asn Val Ala Ile Phe Gly Leu Leu Phe Ser Leu Leu Val 
1               5                   10                  15      


Leu Val Pro Ser Gln Ile Phe Ala Asp Arg Ile Cys Thr Gly Ile Thr 
            20                  25                  30          


Ser Ser Asn Ser Pro His Val Val Lys Thr Ala Thr Gln Gly Glu Val 
        35                  40                  45              


Asn Val Thr Gly Val Ile Pro Leu Thr Thr Thr Pro Thr Lys Ser His 
    50                  55                  60                  


Phe Ala Asn Leu Lys Gly Thr Glu Thr Arg Gly Lys Leu Cys Pro Lys 
65                  70                  75                  80  


Cys Leu Asn Cys Thr Asp Leu Asp Val Ala Leu Gly Arg Pro Lys Cys 
                85                  90                  95      


Thr Gly Lys Ile Pro Ser Ala Arg Val Ser Ile Leu His Glu Val Arg 
            100                 105                 110         


Pro Val Thr Ser Gly Cys Phe Pro Ile Met His Asp Arg Thr Lys Ile 
        115                 120                 125             


Arg Gln Leu Pro Asn Leu Leu Arg Gly Tyr Glu His Val Arg Leu Ser 
    130                 135                 140                 


Thr His Asn Val Ile Asn Ala Glu Asp Ala Pro Gly Gly Pro Tyr Lys 
145                 150                 155                 160 


Ile Gly Thr Ser Gly Ala Cys Pro Asn Ile Thr Asn Gly Asn Gly Phe 
                165                 170                 175     


Phe Ala Thr Met Ala Trp Ala Val Pro Asp Lys Asn Lys Thr Ala Thr 
            180                 185                 190         


Asn Pro Leu Thr Ile Glu Val Pro Tyr Val Cys Thr Glu Gly Glu Asp 
        195                 200                 205             


Gln Ile Thr Val Trp Gly Phe His Ser Asp Asn Glu Thr Gln Met Ala 
    210                 215                 220                 


Lys Leu Tyr Gly Asp Ser Lys Pro Gln Lys Phe Thr Ser Ser Ala Asn 
225                 230                 235                 240 


Gly Val Thr Thr His Tyr Val Ser Gln Ile Gly Gly Phe Pro Asn Gln 
                245                 250                 255     


Thr Glu Asp Gly Gly Leu Pro Gln Ser Gly Arg Ile Val Val Asp Tyr 
            260                 265                 270         


Met Val Gln Lys Ser Gly Lys Thr Gly Thr Ile Thr Tyr Gln Arg Gly 
        275                 280                 285             


Ile Leu Leu Pro Gln Lys Val Trp Cys Ala Ser Gly Arg Ser Lys Val 
    290                 295                 300                 


Ile Lys Gly Ser Leu Pro Leu Ile Gly Glu Ala Asp Cys Leu His Glu 
305                 310                 315                 320 


Lys Tyr Gly Gly Leu Asn Lys Ser Lys Pro Tyr Tyr Thr Gly Glu His 
                325                 330                 335     


Ala Lys Ala Ile Gly Asn Cys Pro Ile Trp Val Lys Thr Pro Leu Lys 
            340                 345                 350         


Leu Ala Asn Gly Thr Lys Tyr Arg Pro Pro Gly Gly Gly Trp Glu Gly 
        355                 360                 365             


Met Ile Ala Gly Trp His Gly Tyr Thr Ser His Gly Ala His Gly Val 
    370                 375                 380                 


Ala Val Ala Ala Asp Leu Lys Ser Thr Gln Glu Ala Ile Asn Lys Ile 
385                 390                 395                 400 


Thr Lys Asn Leu Asn Ser Leu Ser Glu Leu Glu Val Lys Asn Leu Gln 
                405                 410                 415     


Arg Leu Ser Gly Ala Met Asp Glu Leu His Asn Glu Ile Leu Glu Leu 
            420                 425                 430         


Asp Glu Lys Val Asp Asp Leu Arg Ala Asp Thr Ile Ser Ser Gln Ile 
        435                 440                 445             


Glu Leu Ala Val Leu Leu Ser Asn Glu Gly Ile Ile Asn Ser Glu Asp 
    450                 455                 460                 


Glu His Leu Leu Ala Leu Glu Arg Lys Leu Lys Lys Met Leu Gly Pro 
465                 470                 475                 480 


Ser Ala Val Glu Ile Gly Asn Gly Cys Phe Glu Thr Lys His Lys Cys 
                485                 490                 495     


Asn Gln Thr Cys Leu Asp Lys Ile Ala Ala Gly Thr Phe Asp Ala Gly 
            500                 505                 510         


Glu Phe Ser Leu Pro Thr Phe Asp Ser Leu Asn Ile Thr Ala Ala Ser 
        515                 520                 525             


Leu Asn Asp Asp Gly Leu Asp Asn Tyr Gln Ile Leu Ala Ile Tyr Ser 
    530                 535                 540                 


Thr Val Ala Ser Ser Leu Val Leu Val Val Ser Leu Gly Ala Ile Ser 
545                 550                 555                 560 


Phe Trp Met Cys Ser Asn Gly Ser Leu Gln Cys Arg Ile Cys Ile 
                565                 570                 575 


<210>  146
<211>  1728
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PDI-B Mary-D194G DNA

<400>  146
atggcgaaaa acgttgcgat tttcggctta ttgttttctc ttcttgtgtt ggttccttct       60

cagatcttcg cggatcgaat ctgcactggg ataacatcgt caaactcacc acatgtcgtc      120

aaaactgcta ctcaagggga ggtcaatgtg accggtgtaa taccactgac aacaacaccc      180

accaaatctc attttgcaaa tctcaaagga acagaaacca gggggaaact atgcccaaaa      240

tgcctcaact gcacagatct ggatgtagcc ttgggcagac caaaatgcac agggaaaata      300

ccctctgcaa gggtttcaat actccatgaa gtcagacctg ttacatctgg gtgctttcct      360

ataatgcacg atagaacaaa aattagacag ctgcctaacc ttctccgagg atacgaacat      420

gtcaggttat caactcacaa cgttatcaat gcagaagacg caccaggagg accctacaaa      480

attggaacct cagggtcttg ccctaacatt accaatggaa acggattctt cgcaacaatg      540

gcttgggccg tcccagacaa aaacaaaaca gcaacaaatc cattaacaat agaagtacca      600

tacgtgtgta cagaaggaga agaccaaatt accgtttggg ggttccactc tggcaacgag      660

acccaaatgg caaagctcta tggggactca aagccccaga agttcacctc atctgccaac      720

ggagtgacca cacattacgt ttcacagatt ggtggcttcc caaatcaaac agaagacgga      780

ggactaccac aaagtggcag aattgttgtt gattacatgg tgcaaaaatc tggaaaaaca      840

ggaacaatta cctatcaaag aggtatttta ttgcctcaaa aggtgtggtg cgcaagtggc      900

aggagcaagg taataaaagg atccttgcct ttaattggag aagcagattg cctccatgaa      960

aaatacggtg gattaaacaa aagcaagcct tactacacag gggaacatgc aaaggccata     1020

ggaaattgcc caatatgggt gaaaacaccc ttgaagctgg ccaatggaac caaatataga     1080

cctcctggtg gaggatggga aggaatgatt gcaggttggc acggatacac atcccatggg     1140

gcacatggag tagcggtggc agcagacctt aagagcactc aagaggccat aaacaagata     1200

acaaaaaatc tcaactcttt gagtgagctg gaagtaaaga atcttcaaag actaagcggt     1260

gccatggatg aactccacaa cgaaatacta gaactagatg agaaagtgga tgatctcaga     1320

gctgatacaa taagctcaca aatagaactc gcagtcctgc tttccaatga aggaataata     1380

aacagtgaag atgaacatct cttggcgctt gaaagaaagc tgaagaaaat gctgggcccc     1440

tctgctgtag agatagggaa tggatgcttt gaaaccaaac acaagtgcaa ccagacctgt     1500

ctcgacaaga tagctgctgg tacctttgat gcaggagaat tttctctccc cacctttgat     1560

tcactgaata ttactgctgc atctttaaat gacgatggat tggataatta ccagattttg     1620

gcgatctatt caactgtcgc cagttcattg gtactggtag tctccctggg ggcaatcagt     1680

ttctggatgt gctctaatgg gtctctacag tgtagaatat gtatttaa                  1728


<210>  147
<211>  575
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  PDI-B Mary-D194G AA

<400>  147

Met Ala Lys Asn Val Ala Ile Phe Gly Leu Leu Phe Ser Leu Leu Val 
1               5                   10                  15      


Leu Val Pro Ser Gln Ile Phe Ala Asp Arg Ile Cys Thr Gly Ile Thr 
            20                  25                  30          


Ser Ser Asn Ser Pro His Val Val Lys Thr Ala Thr Gln Gly Glu Val 
        35                  40                  45              


Asn Val Thr Gly Val Ile Pro Leu Thr Thr Thr Pro Thr Lys Ser His 
    50                  55                  60                  


Phe Ala Asn Leu Lys Gly Thr Glu Thr Arg Gly Lys Leu Cys Pro Lys 
65                  70                  75                  80  


Cys Leu Asn Cys Thr Asp Leu Asp Val Ala Leu Gly Arg Pro Lys Cys 
                85                  90                  95      


Thr Gly Lys Ile Pro Ser Ala Arg Val Ser Ile Leu His Glu Val Arg 
            100                 105                 110         


Pro Val Thr Ser Gly Cys Phe Pro Ile Met His Asp Arg Thr Lys Ile 
        115                 120                 125             


Arg Gln Leu Pro Asn Leu Leu Arg Gly Tyr Glu His Val Arg Leu Ser 
    130                 135                 140                 


Thr His Asn Val Ile Asn Ala Glu Asp Ala Pro Gly Gly Pro Tyr Lys 
145                 150                 155                 160 


Ile Gly Thr Ser Gly Ser Cys Pro Asn Ile Thr Asn Gly Asn Gly Phe 
                165                 170                 175     


Phe Ala Thr Met Ala Trp Ala Val Pro Asp Lys Asn Lys Thr Ala Thr 
            180                 185                 190         


Asn Pro Leu Thr Ile Glu Val Pro Tyr Val Cys Thr Glu Gly Glu Asp 
        195                 200                 205             


Gln Ile Thr Val Trp Gly Phe His Ser Gly Asn Glu Thr Gln Met Ala 
    210                 215                 220                 


Lys Leu Tyr Gly Asp Ser Lys Pro Gln Lys Phe Thr Ser Ser Ala Asn 
225                 230                 235                 240 


Gly Val Thr Thr His Tyr Val Ser Gln Ile Gly Gly Phe Pro Asn Gln 
                245                 250                 255     


Thr Glu Asp Gly Gly Leu Pro Gln Ser Gly Arg Ile Val Val Asp Tyr 
            260                 265                 270         


Met Val Gln Lys Ser Gly Lys Thr Gly Thr Ile Thr Tyr Gln Arg Gly 
        275                 280                 285             


Ile Leu Leu Pro Gln Lys Val Trp Cys Ala Ser Gly Arg Ser Lys Val 
    290                 295                 300                 


Ile Lys Gly Ser Leu Pro Leu Ile Gly Glu Ala Asp Cys Leu His Glu 
305                 310                 315                 320 


Lys Tyr Gly Gly Leu Asn Lys Ser Lys Pro Tyr Tyr Thr Gly Glu His 
                325                 330                 335     


Ala Lys Ala Ile Gly Asn Cys Pro Ile Trp Val Lys Thr Pro Leu Lys 
            340                 345                 350         


Leu Ala Asn Gly Thr Lys Tyr Arg Pro Pro Gly Gly Gly Trp Glu Gly 
        355                 360                 365             


Met Ile Ala Gly Trp His Gly Tyr Thr Ser His Gly Ala His Gly Val 
    370                 375                 380                 


Ala Val Ala Ala Asp Leu Lys Ser Thr Gln Glu Ala Ile Asn Lys Ile 
385                 390                 395                 400 


Thr Lys Asn Leu Asn Ser Leu Ser Glu Leu Glu Val Lys Asn Leu Gln 
                405                 410                 415     


Arg Leu Ser Gly Ala Met Asp Glu Leu His Asn Glu Ile Leu Glu Leu 
            420                 425                 430         


Asp Glu Lys Val Asp Asp Leu Arg Ala Asp Thr Ile Ser Ser Gln Ile 
        435                 440                 445             


Glu Leu Ala Val Leu Leu Ser Asn Glu Gly Ile Ile Asn Ser Glu Asp 
    450                 455                 460                 


Glu His Leu Leu Ala Leu Glu Arg Lys Leu Lys Lys Met Leu Gly Pro 
465                 470                 475                 480 


Ser Ala Val Glu Ile Gly Asn Gly Cys Phe Glu Thr Lys His Lys Cys 
                485                 490                 495     


Asn Gln Thr Cys Leu Asp Lys Ile Ala Ala Gly Thr Phe Asp Ala Gly 
            500                 505                 510         


Glu Phe Ser Leu Pro Thr Phe Asp Ser Leu Asn Ile Thr Ala Ala Ser 
        515                 520                 525             


Leu Asn Asp Asp Gly Leu Asp Asn Tyr Gln Ile Leu Ala Ile Tyr Ser 
    530                 535                 540                 


Thr Val Ala Ser Ser Leu Val Leu Val Val Ser Leu Gly Ala Ile Ser 
545                 550                 555                 560 


Phe Trp Met Cys Ser Asn Gly Ser Leu Gln Cys Arg Ile Cys Ile 
                565                 570                 575 


<210>  148
<211>  1728
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PDI-B Mary-L202A DNA

<400>  148
atggcgaaaa acgttgcgat tttcggctta ttgttttctc ttcttgtgtt ggttccttct       60

cagatcttcg cggatcgaat ctgcactggg ataacatcgt caaactcacc acatgtcgtc      120

aaaactgcta ctcaagggga ggtcaatgtg accggtgtaa taccactgac aacaacaccc      180

accaaatctc attttgcaaa tctcaaagga acagaaacca gggggaaact atgcccaaaa      240

tgcctcaact gcacagatct ggatgtagcc ttgggcagac caaaatgcac agggaaaata      300

ccctctgcaa gggtttcaat actccatgaa gtcagacctg ttacatctgg gtgctttcct      360

ataatgcacg atagaacaaa aattagacag ctgcctaacc ttctccgagg atacgaacat      420

gtcaggttat caactcacaa cgttatcaat gcagaagacg caccaggagg accctacaaa      480

attggaacct cagggtcttg ccctaacatt accaatggaa acggattctt cgcaacaatg      540

gcttgggccg tcccagacaa aaacaaaaca gcaacaaatc cattaacaat agaagtacca      600

tacgtgtgta cagaaggaga agaccaaatt accgtttggg ggttccactc tgacaacgag      660

acccaaatgg caaaggccta tggggactca aagccccaga agttcacctc atctgccaac      720

ggagtgacca cacattacgt ttcacagatt ggtggcttcc caaatcaaac agaagacgga      780

ggactaccac aaagtggcag aattgttgtt gattacatgg tgcaaaaatc tggaaaaaca      840

ggaacaatta cctatcaaag aggtatttta ttgcctcaaa aggtgtggtg cgcaagtggc      900

aggagcaagg taataaaagg atccttgcct ttaattggag aagcagattg cctccatgaa      960

aaatacggtg gattaaacaa aagcaagcct tactacacag gggaacatgc aaaggccata     1020

ggaaattgcc caatatgggt gaaaacaccc ttgaagctgg ccaatggaac caaatataga     1080

cctcctggtg gaggatggga aggaatgatt gcaggttggc acggatacac atcccatggg     1140

gcacatggag tagcggtggc agcagacctt aagagcactc aagaggccat aaacaagata     1200

acaaaaaatc tcaactcttt gagtgagctg gaagtaaaga atcttcaaag actaagcggt     1260

gccatggatg aactccacaa cgaaatacta gaactagatg agaaagtgga tgatctcaga     1320

gctgatacaa taagctcaca aatagaactc gcagtcctgc tttccaatga aggaataata     1380

aacagtgaag atgaacatct cttggcgctt gaaagaaagc tgaagaaaat gctgggcccc     1440

tctgctgtag agatagggaa tggatgcttt gaaaccaaac acaagtgcaa ccagacctgt     1500

ctcgacaaga tagctgctgg tacctttgat gcaggagaat tttctctccc cacctttgat     1560

tcactgaata ttactgctgc atctttaaat gacgatggat tggataatta ccagattttg     1620

gcgatctatt caactgtcgc cagttcattg gtactggtag tctccctggg ggcaatcagt     1680

ttctggatgt gctctaatgg gtctctacag tgtagaatat gtatttaa                  1728


<210>  149
<211>  575
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  PDI-B Mary-L202A AA

<400>  149

Met Ala Lys Asn Val Ala Ile Phe Gly Leu Leu Phe Ser Leu Leu Val 
1               5                   10                  15      


Leu Val Pro Ser Gln Ile Phe Ala Asp Arg Ile Cys Thr Gly Ile Thr 
            20                  25                  30          


Ser Ser Asn Ser Pro His Val Val Lys Thr Ala Thr Gln Gly Glu Val 
        35                  40                  45              


Asn Val Thr Gly Val Ile Pro Leu Thr Thr Thr Pro Thr Lys Ser His 
    50                  55                  60                  


Phe Ala Asn Leu Lys Gly Thr Glu Thr Arg Gly Lys Leu Cys Pro Lys 
65                  70                  75                  80  


Cys Leu Asn Cys Thr Asp Leu Asp Val Ala Leu Gly Arg Pro Lys Cys 
                85                  90                  95      


Thr Gly Lys Ile Pro Ser Ala Arg Val Ser Ile Leu His Glu Val Arg 
            100                 105                 110         


Pro Val Thr Ser Gly Cys Phe Pro Ile Met His Asp Arg Thr Lys Ile 
        115                 120                 125             


Arg Gln Leu Pro Asn Leu Leu Arg Gly Tyr Glu His Val Arg Leu Ser 
    130                 135                 140                 


Thr His Asn Val Ile Asn Ala Glu Asp Ala Pro Gly Gly Pro Tyr Lys 
145                 150                 155                 160 


Ile Gly Thr Ser Gly Ser Cys Pro Asn Ile Thr Asn Gly Asn Gly Phe 
                165                 170                 175     


Phe Ala Thr Met Ala Trp Ala Val Pro Asp Lys Asn Lys Thr Ala Thr 
            180                 185                 190         


Asn Pro Leu Thr Ile Glu Val Pro Tyr Val Cys Thr Glu Gly Glu Asp 
        195                 200                 205             


Gln Ile Thr Val Trp Gly Phe His Ser Asp Asn Glu Thr Gln Met Ala 
    210                 215                 220                 


Lys Ala Tyr Gly Asp Ser Lys Pro Gln Lys Phe Thr Ser Ser Ala Asn 
225                 230                 235                 240 


Gly Val Thr Thr His Tyr Val Ser Gln Ile Gly Gly Phe Pro Asn Gln 
                245                 250                 255     


Thr Glu Asp Gly Gly Leu Pro Gln Ser Gly Arg Ile Val Val Asp Tyr 
            260                 265                 270         


Met Val Gln Lys Ser Gly Lys Thr Gly Thr Ile Thr Tyr Gln Arg Gly 
        275                 280                 285             


Ile Leu Leu Pro Gln Lys Val Trp Cys Ala Ser Gly Arg Ser Lys Val 
    290                 295                 300                 


Ile Lys Gly Ser Leu Pro Leu Ile Gly Glu Ala Asp Cys Leu His Glu 
305                 310                 315                 320 


Lys Tyr Gly Gly Leu Asn Lys Ser Lys Pro Tyr Tyr Thr Gly Glu His 
                325                 330                 335     


Ala Lys Ala Ile Gly Asn Cys Pro Ile Trp Val Lys Thr Pro Leu Lys 
            340                 345                 350         


Leu Ala Asn Gly Thr Lys Tyr Arg Pro Pro Gly Gly Gly Trp Glu Gly 
        355                 360                 365             


Met Ile Ala Gly Trp His Gly Tyr Thr Ser His Gly Ala His Gly Val 
    370                 375                 380                 


Ala Val Ala Ala Asp Leu Lys Ser Thr Gln Glu Ala Ile Asn Lys Ile 
385                 390                 395                 400 


Thr Lys Asn Leu Asn Ser Leu Ser Glu Leu Glu Val Lys Asn Leu Gln 
                405                 410                 415     


Arg Leu Ser Gly Ala Met Asp Glu Leu His Asn Glu Ile Leu Glu Leu 
            420                 425                 430         


Asp Glu Lys Val Asp Asp Leu Arg Ala Asp Thr Ile Ser Ser Gln Ile 
        435                 440                 445             


Glu Leu Ala Val Leu Leu Ser Asn Glu Gly Ile Ile Asn Ser Glu Asp 
    450                 455                 460                 


Glu His Leu Leu Ala Leu Glu Arg Lys Leu Lys Lys Met Leu Gly Pro 
465                 470                 475                 480 


Ser Ala Val Glu Ile Gly Asn Gly Cys Phe Glu Thr Lys His Lys Cys 
                485                 490                 495     


Asn Gln Thr Cys Leu Asp Lys Ile Ala Ala Gly Thr Phe Asp Ala Gly 
            500                 505                 510         


Glu Phe Ser Leu Pro Thr Phe Asp Ser Leu Asn Ile Thr Ala Ala Ser 
        515                 520                 525             


Leu Asn Asp Asp Gly Leu Asp Asn Tyr Gln Ile Leu Ala Ile Tyr Ser 
    530                 535                 540                 


Thr Val Ala Ser Ser Leu Val Leu Val Val Ser Leu Gly Ala Ile Ser 
545                 550                 555                 560 


Phe Trp Met Cys Ser Asn Gly Ser Leu Gln Cys Arg Ile Cys Ile 
                565                 570                 575 


<210>  150
<211>  1728
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PDI-B Mary-L202W DNA

<400>  150
atggcgaaaa acgttgcgat tttcggctta ttgttttctc ttcttgtgtt ggttccttct       60

cagatcttcg cggatcgaat ctgcactggg ataacatcgt caaactcacc acatgtcgtc      120

aaaactgcta ctcaagggga ggtcaatgtg accggtgtaa taccactgac aacaacaccc      180

accaaatctc attttgcaaa tctcaaagga acagaaacca gggggaaact atgcccaaaa      240

tgcctcaact gcacagatct ggatgtagcc ttgggcagac caaaatgcac agggaaaata      300

ccctctgcaa gggtttcaat actccatgaa gtcagacctg ttacatctgg gtgctttcct      360

ataatgcacg atagaacaaa aattagacag ctgcctaacc ttctccgagg atacgaacat      420

gtcaggttat caactcacaa cgttatcaat gcagaagacg caccaggagg accctacaaa      480

attggaacct cagggtcttg ccctaacatt accaatggaa acggattctt cgcaacaatg      540

gcttgggccg tcccagacaa aaacaaaaca gcaacaaatc cattaacaat agaagtacca      600

tacgtgtgta cagaaggaga agaccaaatt accgtttggg ggttccactc tgacaacgag      660

acccaaatgg caaagtggta tggggactca aagccccaga agttcacctc atctgccaac      720

ggagtgacca cacattacgt ttcacagatt ggtggcttcc caaatcaaac agaagacgga      780

ggactaccac aaagtggcag aattgttgtt gattacatgg tgcaaaaatc tggaaaaaca      840

ggaacaatta cctatcaaag aggtatttta ttgcctcaaa aggtgtggtg cgcaagtggc      900

aggagcaagg taataaaagg atccttgcct ttaattggag aagcagattg cctccatgaa      960

aaatacggtg gattaaacaa aagcaagcct tactacacag gggaacatgc aaaggccata     1020

ggaaattgcc caatatgggt gaaaacaccc ttgaagctgg ccaatggaac caaatataga     1080

cctcctggtg gaggatggga aggaatgatt gcaggttggc acggatacac atcccatggg     1140

gcacatggag tagcggtggc agcagacctt aagagcactc aagaggccat aaacaagata     1200

acaaaaaatc tcaactcttt gagtgagctg gaagtaaaga atcttcaaag actaagcggt     1260

gccatggatg aactccacaa cgaaatacta gaactagatg agaaagtgga tgatctcaga     1320

gctgatacaa taagctcaca aatagaactc gcagtcctgc tttccaatga aggaataata     1380

aacagtgaag atgaacatct cttggcgctt gaaagaaagc tgaagaaaat gctgggcccc     1440

tctgctgtag agatagggaa tggatgcttt gaaaccaaac acaagtgcaa ccagacctgt     1500

ctcgacaaga tagctgctgg tacctttgat gcaggagaat tttctctccc cacctttgat     1560

tcactgaata ttactgctgc atctttaaat gacgatggat tggataatta ccagattttg     1620

gcgatctatt caactgtcgc cagttcattg gtactggtag tctccctggg ggcaatcagt     1680

ttctggatgt gctctaatgg gtctctacag tgtagaatat gtatttaa                  1728


<210>  151
<211>  575
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  PDI-B Mary-L202W AA

<400>  151

Met Ala Lys Asn Val Ala Ile Phe Gly Leu Leu Phe Ser Leu Leu Val 
1               5                   10                  15      


Leu Val Pro Ser Gln Ile Phe Ala Asp Arg Ile Cys Thr Gly Ile Thr 
            20                  25                  30          


Ser Ser Asn Ser Pro His Val Val Lys Thr Ala Thr Gln Gly Glu Val 
        35                  40                  45              


Asn Val Thr Gly Val Ile Pro Leu Thr Thr Thr Pro Thr Lys Ser His 
    50                  55                  60                  


Phe Ala Asn Leu Lys Gly Thr Glu Thr Arg Gly Lys Leu Cys Pro Lys 
65                  70                  75                  80  


Cys Leu Asn Cys Thr Asp Leu Asp Val Ala Leu Gly Arg Pro Lys Cys 
                85                  90                  95      


Thr Gly Lys Ile Pro Ser Ala Arg Val Ser Ile Leu His Glu Val Arg 
            100                 105                 110         


Pro Val Thr Ser Gly Cys Phe Pro Ile Met His Asp Arg Thr Lys Ile 
        115                 120                 125             


Arg Gln Leu Pro Asn Leu Leu Arg Gly Tyr Glu His Val Arg Leu Ser 
    130                 135                 140                 


Thr His Asn Val Ile Asn Ala Glu Asp Ala Pro Gly Gly Pro Tyr Lys 
145                 150                 155                 160 


Ile Gly Thr Ser Gly Ser Cys Pro Asn Ile Thr Asn Gly Asn Gly Phe 
                165                 170                 175     


Phe Ala Thr Met Ala Trp Ala Val Pro Asp Lys Asn Lys Thr Ala Thr 
            180                 185                 190         


Asn Pro Leu Thr Ile Glu Val Pro Tyr Val Cys Thr Glu Gly Glu Asp 
        195                 200                 205             


Gln Ile Thr Val Trp Gly Phe His Ser Asp Asn Glu Thr Gln Met Ala 
    210                 215                 220                 


Lys Trp Tyr Gly Asp Ser Lys Pro Gln Lys Phe Thr Ser Ser Ala Asn 
225                 230                 235                 240 


Gly Val Thr Thr His Tyr Val Ser Gln Ile Gly Gly Phe Pro Asn Gln 
                245                 250                 255     


Thr Glu Asp Gly Gly Leu Pro Gln Ser Gly Arg Ile Val Val Asp Tyr 
            260                 265                 270         


Met Val Gln Lys Ser Gly Lys Thr Gly Thr Ile Thr Tyr Gln Arg Gly 
        275                 280                 285             


Ile Leu Leu Pro Gln Lys Val Trp Cys Ala Ser Gly Arg Ser Lys Val 
    290                 295                 300                 


Ile Lys Gly Ser Leu Pro Leu Ile Gly Glu Ala Asp Cys Leu His Glu 
305                 310                 315                 320 


Lys Tyr Gly Gly Leu Asn Lys Ser Lys Pro Tyr Tyr Thr Gly Glu His 
                325                 330                 335     


Ala Lys Ala Ile Gly Asn Cys Pro Ile Trp Val Lys Thr Pro Leu Lys 
            340                 345                 350         


Leu Ala Asn Gly Thr Lys Tyr Arg Pro Pro Gly Gly Gly Trp Glu Gly 
        355                 360                 365             


Met Ile Ala Gly Trp His Gly Tyr Thr Ser His Gly Ala His Gly Val 
    370                 375                 380                 


Ala Val Ala Ala Asp Leu Lys Ser Thr Gln Glu Ala Ile Asn Lys Ile 
385                 390                 395                 400 


Thr Lys Asn Leu Asn Ser Leu Ser Glu Leu Glu Val Lys Asn Leu Gln 
                405                 410                 415     


Arg Leu Ser Gly Ala Met Asp Glu Leu His Asn Glu Ile Leu Glu Leu 
            420                 425                 430         


Asp Glu Lys Val Asp Asp Leu Arg Ala Asp Thr Ile Ser Ser Gln Ile 
        435                 440                 445             


Glu Leu Ala Val Leu Leu Ser Asn Glu Gly Ile Ile Asn Ser Glu Asp 
    450                 455                 460                 


Glu His Leu Leu Ala Leu Glu Arg Lys Leu Lys Lys Met Leu Gly Pro 
465                 470                 475                 480 


Ser Ala Val Glu Ile Gly Asn Gly Cys Phe Glu Thr Lys His Lys Cys 
                485                 490                 495     


Asn Gln Thr Cys Leu Asp Lys Ile Ala Ala Gly Thr Phe Asp Ala Gly 
            500                 505                 510         


Glu Phe Ser Leu Pro Thr Phe Asp Ser Leu Asn Ile Thr Ala Ala Ser 
        515                 520                 525             


Leu Asn Asp Asp Gly Leu Asp Asn Tyr Gln Ile Leu Ala Ile Tyr Ser 
    530                 535                 540                 


Thr Val Ala Ser Ser Leu Val Leu Val Val Ser Leu Gly Ala Ile Ser 
545                 550                 555                 560 


Phe Trp Met Cys Ser Asn Gly Ser Leu Gln Cys Arg Ile Cys Ile 
                565                 570                 575 


<210>  152
<211>  1725
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PDI-B Wash DNA

<400>  152
atggcgaaaa acgttgcgat tttcggctta ttgttttctc ttcttgtgtt ggttccttct       60

cagatcttcg cggatcgaat ctgcactggg ataacatcgt caaactcacc acatgtcgtc      120

aaaactgcta ctcaagggga ggtcaatgtg accggtgtaa taccactgac aacaacaccc      180

accaaatctc attttgcaaa tctcaaagga acagaaacca gggggaaact atgcccaaaa      240

tgcctcaact gcacagatct ggatgtagcc ttgggcagac caaaatgcac agggaaaata      300

ccctctgcaa gggtttcaat actccatgaa gtcagacctg ttacatctgg gtgctttcct      360

ataatgcacg atagaacaaa aattagacag ctgcctaacc ttctccgagg atacgaacat      420

gtcaggttat caactcacaa cgttatcaat gcagaagatg caccaggaag accctacgag      480

attggaacct cagggtcttg ccctaacatt accaatggaa acggattctt cgcaacaatg      540

gcttgggccg tcccaaaaaa caaaacagca acaaatccat taacaataga agtaccatac      600

atttgtacag aaggagaaga ccaaattacc gtttgggggt tccactctga caacgagacc      660

caaatggcaa agctctatgg ggactcaaag ccccagaagt tcacctcatc tgccaacgga      720

gtgaccacac attacgtttc acagattggt ggcttcccaa atcaaacaga agacggagga      780

ctaccacaaa gtggcagaat tgttgttgat tacatggtgc aaaaatctgg aaaaacagga      840

acaattacct atcaaagagg tattttattg cctcaaaagg tgtggtgcgc aagtggcagg      900

agcaaggtaa taaaaggatc cttgccttta attggagaag cagattgcct ccatgaaaaa      960

tacggtggat taaacaaaag caagccttac tacacagggg aacatgcaaa ggccatagga     1020

aattgcccaa tatgggtgaa aacacccttg aagctggcca atggaaccaa atatagacct     1080

cctggtggag gatgggaagg aatgattgca ggttggcacg gatacacatc ccatggggca     1140

catggagtag cggtggcagc agaccttaag agcactcaag aggccataaa caagataaca     1200

aaaaatctca actctttgag tgagctggaa gtaaagaatc ttcaaagact aagcggtgcc     1260

atggatgaac tccacaacga aatactagaa ctagatgaga aagtggatga tctcagagct     1320

gatacaataa gctcacaaat agaactcgca gtcctgcttt ccaatgaagg aataataaac     1380

agtgaagatg aacatctctt ggcgcttgaa agaaagctga agaaaatgct gggcccctct     1440

gctgtagaga tagggaatgg atgctttgaa accaaacaca agtgcaacca gacctgtctc     1500

gacagaatag ctgctggtac ctttgatgca ggagaatttt ctctccccac ctttgattca     1560

ctgaatatta ctgctgcatc tttaaatgac gatggattgg ataattacca gattttggcg     1620

atctattcaa ctgtcgccag ttcattggta ctggtagtct ccctgggggc aatcagtttc     1680

tggatgtgct ctaatgggtc tctacagtgt agaatatgta tttaa                     1725


<210>  153
<211>  574
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  PDI-B Wash AA

<400>  153

Met Ala Lys Asn Val Ala Ile Phe Gly Leu Leu Phe Ser Leu Leu Val 
1               5                   10                  15      


Leu Val Pro Ser Gln Ile Phe Ala Asp Arg Ile Cys Thr Gly Ile Thr 
            20                  25                  30          


Ser Ser Asn Ser Pro His Val Val Lys Thr Ala Thr Gln Gly Glu Val 
        35                  40                  45              


Asn Val Thr Gly Val Ile Pro Leu Thr Thr Thr Pro Thr Lys Ser His 
    50                  55                  60                  


Phe Ala Asn Leu Lys Gly Thr Glu Thr Arg Gly Lys Leu Cys Pro Lys 
65                  70                  75                  80  


Cys Leu Asn Cys Thr Asp Leu Asp Val Ala Leu Gly Arg Pro Lys Cys 
                85                  90                  95      


Thr Gly Lys Ile Pro Ser Ala Arg Val Ser Ile Leu His Glu Val Arg 
            100                 105                 110         


Pro Val Thr Ser Gly Cys Phe Pro Ile Met His Asp Arg Thr Lys Ile 
        115                 120                 125             


Arg Gln Leu Pro Asn Leu Leu Arg Gly Tyr Glu His Val Arg Leu Ser 
    130                 135                 140                 


Thr His Asn Val Ile Asn Ala Glu Asp Ala Pro Gly Arg Pro Tyr Glu 
145                 150                 155                 160 


Ile Gly Thr Ser Gly Ser Cys Pro Asn Ile Thr Asn Gly Asn Gly Phe 
                165                 170                 175     


Phe Ala Thr Met Ala Trp Ala Val Pro Lys Asn Lys Thr Ala Thr Asn 
            180                 185                 190         


Pro Leu Thr Ile Glu Val Pro Tyr Ile Cys Thr Glu Gly Glu Asp Gln 
        195                 200                 205             


Ile Thr Val Trp Gly Phe His Ser Asp Asn Glu Thr Gln Met Ala Lys 
    210                 215                 220                 


Leu Tyr Gly Asp Ser Lys Pro Gln Lys Phe Thr Ser Ser Ala Asn Gly 
225                 230                 235                 240 


Val Thr Thr His Tyr Val Ser Gln Ile Gly Gly Phe Pro Asn Gln Thr 
                245                 250                 255     


Glu Asp Gly Gly Leu Pro Gln Ser Gly Arg Ile Val Val Asp Tyr Met 
            260                 265                 270         


Val Gln Lys Ser Gly Lys Thr Gly Thr Ile Thr Tyr Gln Arg Gly Ile 
        275                 280                 285             


Leu Leu Pro Gln Lys Val Trp Cys Ala Ser Gly Arg Ser Lys Val Ile 
    290                 295                 300                 


Lys Gly Ser Leu Pro Leu Ile Gly Glu Ala Asp Cys Leu His Glu Lys 
305                 310                 315                 320 


Tyr Gly Gly Leu Asn Lys Ser Lys Pro Tyr Tyr Thr Gly Glu His Ala 
                325                 330                 335     


Lys Ala Ile Gly Asn Cys Pro Ile Trp Val Lys Thr Pro Leu Lys Leu 
            340                 345                 350         


Ala Asn Gly Thr Lys Tyr Arg Pro Pro Gly Gly Gly Trp Glu Gly Met 
        355                 360                 365             


Ile Ala Gly Trp His Gly Tyr Thr Ser His Gly Ala His Gly Val Ala 
    370                 375                 380                 


Val Ala Ala Asp Leu Lys Ser Thr Gln Glu Ala Ile Asn Lys Ile Thr 
385                 390                 395                 400 


Lys Asn Leu Asn Ser Leu Ser Glu Leu Glu Val Lys Asn Leu Gln Arg 
                405                 410                 415     


Leu Ser Gly Ala Met Asp Glu Leu His Asn Glu Ile Leu Glu Leu Asp 
            420                 425                 430         


Glu Lys Val Asp Asp Leu Arg Ala Asp Thr Ile Ser Ser Gln Ile Glu 
        435                 440                 445             


Leu Ala Val Leu Leu Ser Asn Glu Gly Ile Ile Asn Ser Glu Asp Glu 
    450                 455                 460                 


His Leu Leu Ala Leu Glu Arg Lys Leu Lys Lys Met Leu Gly Pro Ser 
465                 470                 475                 480 


Ala Val Glu Ile Gly Asn Gly Cys Phe Glu Thr Lys His Lys Cys Asn 
                485                 490                 495     


Gln Thr Cys Leu Asp Arg Ile Ala Ala Gly Thr Phe Asp Ala Gly Glu 
            500                 505                 510         


Phe Ser Leu Pro Thr Phe Asp Ser Leu Asn Ile Thr Ala Ala Ser Leu 
        515                 520                 525             


Asn Asp Asp Gly Leu Asp Asn Tyr Gln Ile Leu Ala Ile Tyr Ser Thr 
    530                 535                 540                 


Val Ala Ser Ser Leu Val Leu Val Val Ser Leu Gly Ala Ile Ser Phe 
545                 550                 555                 560 


Trp Met Cys Ser Asn Gly Ser Leu Gln Cys Arg Ile Cys Ile 
                565                 570                 


<210>  154
<211>  1725
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PDI-B Wash-G138A DNA

<400>  154
atggcgaaaa acgttgcgat tttcggctta ttgttttctc ttcttgtgtt ggttccttct       60

cagatcttcg cggatcgaat ctgcactggg ataacatcgt caaactcacc acatgtcgtc      120

aaaactgcta ctcaagggga ggtcaatgtg accggtgtaa taccactgac aacaacaccc      180

accaaatctc attttgcaaa tctcaaagga acagaaacca gggggaaact atgcccaaaa      240

tgcctcaact gcacagatct ggatgtagcc ttgggcagac caaaatgcac agggaaaata      300

ccctctgcaa gggtttcaat actccatgaa gtcagacctg ttacatctgg gtgctttcct      360

ataatgcacg atagaacaaa aattagacag ctgcctaacc ttctccgagg atacgaacat      420

gtcaggttat caactcacaa cgttatcaat gcagaagatg caccaggaag accctacgag      480

attgccacct cagggtcttg ccctaacatt accaatggaa acggattctt cgcaacaatg      540

gcttgggccg tcccaaaaaa caaaacagca acaaatccat taacaataga agtaccatac      600

atttgtacag aaggagaaga ccaaattacc gtttgggggt tccactctga caacgagacc      660

caaatggcaa agctctatgg ggactcaaag ccccagaagt tcacctcatc tgccaacgga      720

gtgaccacac attacgtttc acagattggt ggcttcccaa atcaaacaga agacggagga      780

ctaccacaaa gtggcagaat tgttgttgat tacatggtgc aaaaatctgg aaaaacagga      840

acaattacct atcaaagagg tattttattg cctcaaaagg tgtggtgcgc aagtggcagg      900

agcaaggtaa taaaaggatc cttgccttta attggagaag cagattgcct ccatgaaaaa      960

tacggtggat taaacaaaag caagccttac tacacagggg aacatgcaaa ggccatagga     1020

aattgcccaa tatgggtgaa aacacccttg aagctggcca atggaaccaa atatagacct     1080

cctggtggag gatgggaagg aatgattgca ggttggcacg gatacacatc ccatggggca     1140

catggagtag cggtggcagc agaccttaag agcactcaag aggccataaa caagataaca     1200

aaaaatctca actctttgag tgagctggaa gtaaagaatc ttcaaagact aagcggtgcc     1260

atggatgaac tccacaacga aatactagaa ctagatgaga aagtggatga tctcagagct     1320

gatacaataa gctcacaaat agaactcgca gtcctgcttt ccaatgaagg aataataaac     1380

agtgaagatg aacatctctt ggcgcttgaa agaaagctga agaaaatgct gggcccctct     1440

gctgtagaga tagggaatgg atgctttgaa accaaacaca agtgcaacca gacctgtctc     1500

gacagaatag ctgctggtac ctttgatgca ggagaatttt ctctccccac ctttgattca     1560

ctgaatatta ctgctgcatc tttaaatgac gatggattgg ataattacca gattttggcg     1620

atctattcaa ctgtcgccag ttcattggta ctggtagtct ccctgggggc aatcagtttc     1680

tggatgtgct ctaatgggtc tctacagtgt agaatatgta tttaa                     1725


<210>  155
<211>  574
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  PDI-B Wash-G138A AA

<400>  155

Met Ala Lys Asn Val Ala Ile Phe Gly Leu Leu Phe Ser Leu Leu Val 
1               5                   10                  15      


Leu Val Pro Ser Gln Ile Phe Ala Asp Arg Ile Cys Thr Gly Ile Thr 
            20                  25                  30          


Ser Ser Asn Ser Pro His Val Val Lys Thr Ala Thr Gln Gly Glu Val 
        35                  40                  45              


Asn Val Thr Gly Val Ile Pro Leu Thr Thr Thr Pro Thr Lys Ser His 
    50                  55                  60                  


Phe Ala Asn Leu Lys Gly Thr Glu Thr Arg Gly Lys Leu Cys Pro Lys 
65                  70                  75                  80  


Cys Leu Asn Cys Thr Asp Leu Asp Val Ala Leu Gly Arg Pro Lys Cys 
                85                  90                  95      


Thr Gly Lys Ile Pro Ser Ala Arg Val Ser Ile Leu His Glu Val Arg 
            100                 105                 110         


Pro Val Thr Ser Gly Cys Phe Pro Ile Met His Asp Arg Thr Lys Ile 
        115                 120                 125             


Arg Gln Leu Pro Asn Leu Leu Arg Gly Tyr Glu His Val Arg Leu Ser 
    130                 135                 140                 


Thr His Asn Val Ile Asn Ala Glu Asp Ala Pro Gly Arg Pro Tyr Glu 
145                 150                 155                 160 


Ile Ala Thr Ser Gly Ser Cys Pro Asn Ile Thr Asn Gly Asn Gly Phe 
                165                 170                 175     


Phe Ala Thr Met Ala Trp Ala Val Pro Lys Asn Lys Thr Ala Thr Asn 
            180                 185                 190         


Pro Leu Thr Ile Glu Val Pro Tyr Ile Cys Thr Glu Gly Glu Asp Gln 
        195                 200                 205             


Ile Thr Val Trp Gly Phe His Ser Asp Asn Glu Thr Gln Met Ala Lys 
    210                 215                 220                 


Leu Tyr Gly Asp Ser Lys Pro Gln Lys Phe Thr Ser Ser Ala Asn Gly 
225                 230                 235                 240 


Val Thr Thr His Tyr Val Ser Gln Ile Gly Gly Phe Pro Asn Gln Thr 
                245                 250                 255     


Glu Asp Gly Gly Leu Pro Gln Ser Gly Arg Ile Val Val Asp Tyr Met 
            260                 265                 270         


Val Gln Lys Ser Gly Lys Thr Gly Thr Ile Thr Tyr Gln Arg Gly Ile 
        275                 280                 285             


Leu Leu Pro Gln Lys Val Trp Cys Ala Ser Gly Arg Ser Lys Val Ile 
    290                 295                 300                 


Lys Gly Ser Leu Pro Leu Ile Gly Glu Ala Asp Cys Leu His Glu Lys 
305                 310                 315                 320 


Tyr Gly Gly Leu Asn Lys Ser Lys Pro Tyr Tyr Thr Gly Glu His Ala 
                325                 330                 335     


Lys Ala Ile Gly Asn Cys Pro Ile Trp Val Lys Thr Pro Leu Lys Leu 
            340                 345                 350         


Ala Asn Gly Thr Lys Tyr Arg Pro Pro Gly Gly Gly Trp Glu Gly Met 
        355                 360                 365             


Ile Ala Gly Trp His Gly Tyr Thr Ser His Gly Ala His Gly Val Ala 
    370                 375                 380                 


Val Ala Ala Asp Leu Lys Ser Thr Gln Glu Ala Ile Asn Lys Ile Thr 
385                 390                 395                 400 


Lys Asn Leu Asn Ser Leu Ser Glu Leu Glu Val Lys Asn Leu Gln Arg 
                405                 410                 415     


Leu Ser Gly Ala Met Asp Glu Leu His Asn Glu Ile Leu Glu Leu Asp 
            420                 425                 430         


Glu Lys Val Asp Asp Leu Arg Ala Asp Thr Ile Ser Ser Gln Ile Glu 
        435                 440                 445             


Leu Ala Val Leu Leu Ser Asn Glu Gly Ile Ile Asn Ser Glu Asp Glu 
    450                 455                 460                 


His Leu Leu Ala Leu Glu Arg Lys Leu Lys Lys Met Leu Gly Pro Ser 
465                 470                 475                 480 


Ala Val Glu Ile Gly Asn Gly Cys Phe Glu Thr Lys His Lys Cys Asn 
                485                 490                 495     


Gln Thr Cys Leu Asp Arg Ile Ala Ala Gly Thr Phe Asp Ala Gly Glu 
            500                 505                 510         


Phe Ser Leu Pro Thr Phe Asp Ser Leu Asn Ile Thr Ala Ala Ser Leu 
        515                 520                 525             


Asn Asp Asp Gly Leu Asp Asn Tyr Gln Ile Leu Ala Ile Tyr Ser Thr 
    530                 535                 540                 


Val Ala Ser Ser Leu Val Leu Val Val Ser Leu Gly Ala Ile Ser Phe 
545                 550                 555                 560 


Trp Met Cys Ser Asn Gly Ser Leu Gln Cys Arg Ile Cys Ile 
                565                 570                 


<210>  156
<211>  1725
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PDI-B Wash-S140A DNA

<400>  156
atggcgaaaa acgttgcgat tttcggctta ttgttttctc ttcttgtgtt ggttccttct       60

cagatcttcg cggatcgaat ctgcactggg ataacatcgt caaactcacc acatgtcgtc      120

aaaactgcta ctcaagggga ggtcaatgtg accggtgtaa taccactgac aacaacaccc      180

accaaatctc attttgcaaa tctcaaagga acagaaacca gggggaaact atgcccaaaa      240

tgcctcaact gcacagatct ggatgtagcc ttgggcagac caaaatgcac agggaaaata      300

ccctctgcaa gggtttcaat actccatgaa gtcagacctg ttacatctgg gtgctttcct      360

ataatgcacg atagaacaaa aattagacag ctgcctaacc ttctccgagg atacgaacat      420

gtcaggttat caactcacaa cgttatcaat gcagaagatg caccaggaag accctacgag      480

attggaaccg ccgggtcttg ccctaacatt accaatggaa acggattctt cgcaacaatg      540

gcttgggccg tcccaaaaaa caaaacagca acaaatccat taacaataga agtaccatac      600

atttgtacag aaggagaaga ccaaattacc gtttgggggt tccactctga caacgagacc      660

caaatggcaa agctctatgg ggactcaaag ccccagaagt tcacctcatc tgccaacgga      720

gtgaccacac attacgtttc acagattggt ggcttcccaa atcaaacaga agacggagga      780

ctaccacaaa gtggcagaat tgttgttgat tacatggtgc aaaaatctgg aaaaacagga      840

acaattacct atcaaagagg tattttattg cctcaaaagg tgtggtgcgc aagtggcagg      900

agcaaggtaa taaaaggatc cttgccttta attggagaag cagattgcct ccatgaaaaa      960

tacggtggat taaacaaaag caagccttac tacacagggg aacatgcaaa ggccatagga     1020

aattgcccaa tatgggtgaa aacacccttg aagctggcca atggaaccaa atatagacct     1080

cctggtggag gatgggaagg aatgattgca ggttggcacg gatacacatc ccatggggca     1140

catggagtag cggtggcagc agaccttaag agcactcaag aggccataaa caagataaca     1200

aaaaatctca actctttgag tgagctggaa gtaaagaatc ttcaaagact aagcggtgcc     1260

atggatgaac tccacaacga aatactagaa ctagatgaga aagtggatga tctcagagct     1320

gatacaataa gctcacaaat agaactcgca gtcctgcttt ccaatgaagg aataataaac     1380

agtgaagatg aacatctctt ggcgcttgaa agaaagctga agaaaatgct gggcccctct     1440

gctgtagaga tagggaatgg atgctttgaa accaaacaca agtgcaacca gacctgtctc     1500

gacagaatag ctgctggtac ctttgatgca ggagaatttt ctctccccac ctttgattca     1560

ctgaatatta ctgctgcatc tttaaatgac gatggattgg ataattacca gattttggcg     1620

atctattcaa ctgtcgccag ttcattggta ctggtagtct ccctgggggc aatcagtttc     1680

tggatgtgct ctaatgggtc tctacagtgt agaatatgta tttaa                     1725


<210>  157
<211>  574
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  PDI-B Wash-S140A AA

<400>  157

Met Ala Lys Asn Val Ala Ile Phe Gly Leu Leu Phe Ser Leu Leu Val 
1               5                   10                  15      


Leu Val Pro Ser Gln Ile Phe Ala Asp Arg Ile Cys Thr Gly Ile Thr 
            20                  25                  30          


Ser Ser Asn Ser Pro His Val Val Lys Thr Ala Thr Gln Gly Glu Val 
        35                  40                  45              


Asn Val Thr Gly Val Ile Pro Leu Thr Thr Thr Pro Thr Lys Ser His 
    50                  55                  60                  


Phe Ala Asn Leu Lys Gly Thr Glu Thr Arg Gly Lys Leu Cys Pro Lys 
65                  70                  75                  80  


Cys Leu Asn Cys Thr Asp Leu Asp Val Ala Leu Gly Arg Pro Lys Cys 
                85                  90                  95      


Thr Gly Lys Ile Pro Ser Ala Arg Val Ser Ile Leu His Glu Val Arg 
            100                 105                 110         


Pro Val Thr Ser Gly Cys Phe Pro Ile Met His Asp Arg Thr Lys Ile 
        115                 120                 125             


Arg Gln Leu Pro Asn Leu Leu Arg Gly Tyr Glu His Val Arg Leu Ser 
    130                 135                 140                 


Thr His Asn Val Ile Asn Ala Glu Asp Ala Pro Gly Arg Pro Tyr Glu 
145                 150                 155                 160 


Ile Gly Thr Ala Gly Ser Cys Pro Asn Ile Thr Asn Gly Asn Gly Phe 
                165                 170                 175     


Phe Ala Thr Met Ala Trp Ala Val Pro Lys Asn Lys Thr Ala Thr Asn 
            180                 185                 190         


Pro Leu Thr Ile Glu Val Pro Tyr Ile Cys Thr Glu Gly Glu Asp Gln 
        195                 200                 205             


Ile Thr Val Trp Gly Phe His Ser Asp Asn Glu Thr Gln Met Ala Lys 
    210                 215                 220                 


Leu Tyr Gly Asp Ser Lys Pro Gln Lys Phe Thr Ser Ser Ala Asn Gly 
225                 230                 235                 240 


Val Thr Thr His Tyr Val Ser Gln Ile Gly Gly Phe Pro Asn Gln Thr 
                245                 250                 255     


Glu Asp Gly Gly Leu Pro Gln Ser Gly Arg Ile Val Val Asp Tyr Met 
            260                 265                 270         


Val Gln Lys Ser Gly Lys Thr Gly Thr Ile Thr Tyr Gln Arg Gly Ile 
        275                 280                 285             


Leu Leu Pro Gln Lys Val Trp Cys Ala Ser Gly Arg Ser Lys Val Ile 
    290                 295                 300                 


Lys Gly Ser Leu Pro Leu Ile Gly Glu Ala Asp Cys Leu His Glu Lys 
305                 310                 315                 320 


Tyr Gly Gly Leu Asn Lys Ser Lys Pro Tyr Tyr Thr Gly Glu His Ala 
                325                 330                 335     


Lys Ala Ile Gly Asn Cys Pro Ile Trp Val Lys Thr Pro Leu Lys Leu 
            340                 345                 350         


Ala Asn Gly Thr Lys Tyr Arg Pro Pro Gly Gly Gly Trp Glu Gly Met 
        355                 360                 365             


Ile Ala Gly Trp His Gly Tyr Thr Ser His Gly Ala His Gly Val Ala 
    370                 375                 380                 


Val Ala Ala Asp Leu Lys Ser Thr Gln Glu Ala Ile Asn Lys Ile Thr 
385                 390                 395                 400 


Lys Asn Leu Asn Ser Leu Ser Glu Leu Glu Val Lys Asn Leu Gln Arg 
                405                 410                 415     


Leu Ser Gly Ala Met Asp Glu Leu His Asn Glu Ile Leu Glu Leu Asp 
            420                 425                 430         


Glu Lys Val Asp Asp Leu Arg Ala Asp Thr Ile Ser Ser Gln Ile Glu 
        435                 440                 445             


Leu Ala Val Leu Leu Ser Asn Glu Gly Ile Ile Asn Ser Glu Asp Glu 
    450                 455                 460                 


His Leu Leu Ala Leu Glu Arg Lys Leu Lys Lys Met Leu Gly Pro Ser 
465                 470                 475                 480 


Ala Val Glu Ile Gly Asn Gly Cys Phe Glu Thr Lys His Lys Cys Asn 
                485                 490                 495     


Gln Thr Cys Leu Asp Arg Ile Ala Ala Gly Thr Phe Asp Ala Gly Glu 
            500                 505                 510         


Phe Ser Leu Pro Thr Phe Asp Ser Leu Asn Ile Thr Ala Ala Ser Leu 
        515                 520                 525             


Asn Asp Asp Gly Leu Asp Asn Tyr Gln Ile Leu Ala Ile Tyr Ser Thr 
    530                 535                 540                 


Val Ala Ser Ser Leu Val Leu Val Val Ser Leu Gly Ala Ile Ser Phe 
545                 550                 555                 560 


Trp Met Cys Ser Asn Gly Ser Leu Gln Cys Arg Ile Cys Ile 
                565                 570                 


<210>  158
<211>  1725
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PDI-B Wash-S142A DNA

<400>  158
atggcgaaaa acgttgcgat tttcggctta ttgttttctc ttcttgtgtt ggttccttct       60

cagatcttcg cggatcgaat ctgcactggg ataacatcgt caaactcacc acatgtcgtc      120

aaaactgcta ctcaagggga ggtcaatgtg accggtgtaa taccactgac aacaacaccc      180

accaaatctc attttgcaaa tctcaaagga acagaaacca gggggaaact atgcccaaaa      240

tgcctcaact gcacagatct ggatgtagcc ttgggcagac caaaatgcac agggaaaata      300

ccctctgcaa gggtttcaat actccatgaa gtcagacctg ttacatctgg gtgctttcct      360

ataatgcacg atagaacaaa aattagacag ctgcctaacc ttctccgagg atacgaacat      420

gtcaggttat caactcacaa cgttatcaat gcagaagatg caccaggaag accctacgag      480

attggaacct caggggcctg ccctaacatt accaatggaa acggattctt cgcaacaatg      540

gcttgggccg tcccaaaaaa caaaacagca acaaatccat taacaataga agtaccatac      600

atttgtacag aaggagaaga ccaaattacc gtttgggggt tccactctga caacgagacc      660

caaatggcaa agctctatgg ggactcaaag ccccagaagt tcacctcatc tgccaacgga      720

gtgaccacac attacgtttc acagattggt ggcttcccaa atcaaacaga agacggagga      780

ctaccacaaa gtggcagaat tgttgttgat tacatggtgc aaaaatctgg aaaaacagga      840

acaattacct atcaaagagg tattttattg cctcaaaagg tgtggtgcgc aagtggcagg      900

agcaaggtaa taaaaggatc cttgccttta attggagaag cagattgcct ccatgaaaaa      960

tacggtggat taaacaaaag caagccttac tacacagggg aacatgcaaa ggccatagga     1020

aattgcccaa tatgggtgaa aacacccttg aagctggcca atggaaccaa atatagacct     1080

cctggtggag gatgggaagg aatgattgca ggttggcacg gatacacatc ccatggggca     1140

catggagtag cggtggcagc agaccttaag agcactcaag aggccataaa caagataaca     1200

aaaaatctca actctttgag tgagctggaa gtaaagaatc ttcaaagact aagcggtgcc     1260

atggatgaac tccacaacga aatactagaa ctagatgaga aagtggatga tctcagagct     1320

gatacaataa gctcacaaat agaactcgca gtcctgcttt ccaatgaagg aataataaac     1380

agtgaagatg aacatctctt ggcgcttgaa agaaagctga agaaaatgct gggcccctct     1440

gctgtagaga tagggaatgg atgctttgaa accaaacaca agtgcaacca gacctgtctc     1500

gacagaatag ctgctggtac ctttgatgca ggagaatttt ctctccccac ctttgattca     1560

ctgaatatta ctgctgcatc tttaaatgac gatggattgg ataattacca gattttggcg     1620

atctattcaa ctgtcgccag ttcattggta ctggtagtct ccctgggggc aatcagtttc     1680

tggatgtgct ctaatgggtc tctacagtgt agaatatgta tttaa                     1725


<210>  159
<211>  574
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  PDI-B Wash-S142A AA

<400>  159

Met Ala Lys Asn Val Ala Ile Phe Gly Leu Leu Phe Ser Leu Leu Val 
1               5                   10                  15      


Leu Val Pro Ser Gln Ile Phe Ala Asp Arg Ile Cys Thr Gly Ile Thr 
            20                  25                  30          


Ser Ser Asn Ser Pro His Val Val Lys Thr Ala Thr Gln Gly Glu Val 
        35                  40                  45              


Asn Val Thr Gly Val Ile Pro Leu Thr Thr Thr Pro Thr Lys Ser His 
    50                  55                  60                  


Phe Ala Asn Leu Lys Gly Thr Glu Thr Arg Gly Lys Leu Cys Pro Lys 
65                  70                  75                  80  


Cys Leu Asn Cys Thr Asp Leu Asp Val Ala Leu Gly Arg Pro Lys Cys 
                85                  90                  95      


Thr Gly Lys Ile Pro Ser Ala Arg Val Ser Ile Leu His Glu Val Arg 
            100                 105                 110         


Pro Val Thr Ser Gly Cys Phe Pro Ile Met His Asp Arg Thr Lys Ile 
        115                 120                 125             


Arg Gln Leu Pro Asn Leu Leu Arg Gly Tyr Glu His Val Arg Leu Ser 
    130                 135                 140                 


Thr His Asn Val Ile Asn Ala Glu Asp Ala Pro Gly Arg Pro Tyr Glu 
145                 150                 155                 160 


Ile Gly Thr Ser Gly Ala Cys Pro Asn Ile Thr Asn Gly Asn Gly Phe 
                165                 170                 175     


Phe Ala Thr Met Ala Trp Ala Val Pro Lys Asn Lys Thr Ala Thr Asn 
            180                 185                 190         


Pro Leu Thr Ile Glu Val Pro Tyr Ile Cys Thr Glu Gly Glu Asp Gln 
        195                 200                 205             


Ile Thr Val Trp Gly Phe His Ser Asp Asn Glu Thr Gln Met Ala Lys 
    210                 215                 220                 


Leu Tyr Gly Asp Ser Lys Pro Gln Lys Phe Thr Ser Ser Ala Asn Gly 
225                 230                 235                 240 


Val Thr Thr His Tyr Val Ser Gln Ile Gly Gly Phe Pro Asn Gln Thr 
                245                 250                 255     


Glu Asp Gly Gly Leu Pro Gln Ser Gly Arg Ile Val Val Asp Tyr Met 
            260                 265                 270         


Val Gln Lys Ser Gly Lys Thr Gly Thr Ile Thr Tyr Gln Arg Gly Ile 
        275                 280                 285             


Leu Leu Pro Gln Lys Val Trp Cys Ala Ser Gly Arg Ser Lys Val Ile 
    290                 295                 300                 


Lys Gly Ser Leu Pro Leu Ile Gly Glu Ala Asp Cys Leu His Glu Lys 
305                 310                 315                 320 


Tyr Gly Gly Leu Asn Lys Ser Lys Pro Tyr Tyr Thr Gly Glu His Ala 
                325                 330                 335     


Lys Ala Ile Gly Asn Cys Pro Ile Trp Val Lys Thr Pro Leu Lys Leu 
            340                 345                 350         


Ala Asn Gly Thr Lys Tyr Arg Pro Pro Gly Gly Gly Trp Glu Gly Met 
        355                 360                 365             


Ile Ala Gly Trp His Gly Tyr Thr Ser His Gly Ala His Gly Val Ala 
    370                 375                 380                 


Val Ala Ala Asp Leu Lys Ser Thr Gln Glu Ala Ile Asn Lys Ile Thr 
385                 390                 395                 400 


Lys Asn Leu Asn Ser Leu Ser Glu Leu Glu Val Lys Asn Leu Gln Arg 
                405                 410                 415     


Leu Ser Gly Ala Met Asp Glu Leu His Asn Glu Ile Leu Glu Leu Asp 
            420                 425                 430         


Glu Lys Val Asp Asp Leu Arg Ala Asp Thr Ile Ser Ser Gln Ile Glu 
        435                 440                 445             


Leu Ala Val Leu Leu Ser Asn Glu Gly Ile Ile Asn Ser Glu Asp Glu 
    450                 455                 460                 


His Leu Leu Ala Leu Glu Arg Lys Leu Lys Lys Met Leu Gly Pro Ser 
465                 470                 475                 480 


Ala Val Glu Ile Gly Asn Gly Cys Phe Glu Thr Lys His Lys Cys Asn 
                485                 490                 495     


Gln Thr Cys Leu Asp Arg Ile Ala Ala Gly Thr Phe Asp Ala Gly Glu 
            500                 505                 510         


Phe Ser Leu Pro Thr Phe Asp Ser Leu Asn Ile Thr Ala Ala Ser Leu 
        515                 520                 525             


Asn Asp Asp Gly Leu Asp Asn Tyr Gln Ile Leu Ala Ile Tyr Ser Thr 
    530                 535                 540                 


Val Ala Ser Ser Leu Val Leu Val Val Ser Leu Gly Ala Ile Ser Phe 
545                 550                 555                 560 


Trp Met Cys Ser Asn Gly Ser Leu Gln Cys Arg Ile Cys Ile 
                565                 570                 


<210>  160
<211>  1725
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PDI-B Wash-D193G DNA

<400>  160
atggcgaaaa acgttgcgat tttcggctta ttgttttctc ttcttgtgtt ggttccttct       60

cagatcttcg cggatcgaat ctgcactggg ataacatcgt caaactcacc acatgtcgtc      120

aaaactgcta ctcaagggga ggtcaatgtg accggtgtaa taccactgac aacaacaccc      180

accaaatctc attttgcaaa tctcaaagga acagaaacca gggggaaact atgcccaaaa      240

tgcctcaact gcacagatct ggatgtagcc ttgggcagac caaaatgcac agggaaaata      300

ccctctgcaa gggtttcaat actccatgaa gtcagacctg ttacatctgg gtgctttcct      360

ataatgcacg atagaacaaa aattagacag ctgcctaacc ttctccgagg atacgaacat      420

gtcaggttat caactcacaa cgttatcaat gcagaagatg caccaggaag accctacgag      480

attggaacct cagggtcttg ccctaacatt accaatggaa acggattctt cgcaacaatg      540

gcttgggccg tcccaaaaaa caaaacagca acaaatccat taacaataga agtaccatac      600

atttgtacag aaggagaaga ccaaattacc gtttgggggt tccactctgg caacgagacc      660

caaatggcaa agctctatgg ggactcaaag ccccagaagt tcacctcatc tgccaacgga      720

gtgaccacac attacgtttc acagattggt ggcttcccaa atcaaacaga agacggagga      780

ctaccacaaa gtggcagaat tgttgttgat tacatggtgc aaaaatctgg aaaaacagga      840

acaattacct atcaaagagg tattttattg cctcaaaagg tgtggtgcgc aagtggcagg      900

agcaaggtaa taaaaggatc cttgccttta attggagaag cagattgcct ccatgaaaaa      960

tacggtggat taaacaaaag caagccttac tacacagggg aacatgcaaa ggccatagga     1020

aattgcccaa tatgggtgaa aacacccttg aagctggcca atggaaccaa atatagacct     1080

cctggtggag gatgggaagg aatgattgca ggttggcacg gatacacatc ccatggggca     1140

catggagtag cggtggcagc agaccttaag agcactcaag aggccataaa caagataaca     1200

aaaaatctca actctttgag tgagctggaa gtaaagaatc ttcaaagact aagcggtgcc     1260

atggatgaac tccacaacga aatactagaa ctagatgaga aagtggatga tctcagagct     1320

gatacaataa gctcacaaat agaactcgca gtcctgcttt ccaatgaagg aataataaac     1380

agtgaagatg aacatctctt ggcgcttgaa agaaagctga agaaaatgct gggcccctct     1440

gctgtagaga tagggaatgg atgctttgaa accaaacaca agtgcaacca gacctgtctc     1500

gacagaatag ctgctggtac ctttgatgca ggagaatttt ctctccccac ctttgattca     1560

ctgaatatta ctgctgcatc tttaaatgac gatggattgg ataattacca gattttggcg     1620

atctattcaa ctgtcgccag ttcattggta ctggtagtct ccctgggggc aatcagtttc     1680

tggatgtgct ctaatgggtc tctacagtgt agaatatgta tttaa                     1725


<210>  161
<211>  574
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  PDI-B Wash-D193G AA

<400>  161

Met Ala Lys Asn Val Ala Ile Phe Gly Leu Leu Phe Ser Leu Leu Val 
1               5                   10                  15      


Leu Val Pro Ser Gln Ile Phe Ala Asp Arg Ile Cys Thr Gly Ile Thr 
            20                  25                  30          


Ser Ser Asn Ser Pro His Val Val Lys Thr Ala Thr Gln Gly Glu Val 
        35                  40                  45              


Asn Val Thr Gly Val Ile Pro Leu Thr Thr Thr Pro Thr Lys Ser His 
    50                  55                  60                  


Phe Ala Asn Leu Lys Gly Thr Glu Thr Arg Gly Lys Leu Cys Pro Lys 
65                  70                  75                  80  


Cys Leu Asn Cys Thr Asp Leu Asp Val Ala Leu Gly Arg Pro Lys Cys 
                85                  90                  95      


Thr Gly Lys Ile Pro Ser Ala Arg Val Ser Ile Leu His Glu Val Arg 
            100                 105                 110         


Pro Val Thr Ser Gly Cys Phe Pro Ile Met His Asp Arg Thr Lys Ile 
        115                 120                 125             


Arg Gln Leu Pro Asn Leu Leu Arg Gly Tyr Glu His Val Arg Leu Ser 
    130                 135                 140                 


Thr His Asn Val Ile Asn Ala Glu Asp Ala Pro Gly Arg Pro Tyr Glu 
145                 150                 155                 160 


Ile Gly Thr Ser Gly Ser Cys Pro Asn Ile Thr Asn Gly Asn Gly Phe 
                165                 170                 175     


Phe Ala Thr Met Ala Trp Ala Val Pro Lys Asn Lys Thr Ala Thr Asn 
            180                 185                 190         


Pro Leu Thr Ile Glu Val Pro Tyr Ile Cys Thr Glu Gly Glu Asp Gln 
        195                 200                 205             


Ile Thr Val Trp Gly Phe His Ser Gly Asn Glu Thr Gln Met Ala Lys 
    210                 215                 220                 


Leu Tyr Gly Asp Ser Lys Pro Gln Lys Phe Thr Ser Ser Ala Asn Gly 
225                 230                 235                 240 


Val Thr Thr His Tyr Val Ser Gln Ile Gly Gly Phe Pro Asn Gln Thr 
                245                 250                 255     


Glu Asp Gly Gly Leu Pro Gln Ser Gly Arg Ile Val Val Asp Tyr Met 
            260                 265                 270         


Val Gln Lys Ser Gly Lys Thr Gly Thr Ile Thr Tyr Gln Arg Gly Ile 
        275                 280                 285             


Leu Leu Pro Gln Lys Val Trp Cys Ala Ser Gly Arg Ser Lys Val Ile 
    290                 295                 300                 


Lys Gly Ser Leu Pro Leu Ile Gly Glu Ala Asp Cys Leu His Glu Lys 
305                 310                 315                 320 


Tyr Gly Gly Leu Asn Lys Ser Lys Pro Tyr Tyr Thr Gly Glu His Ala 
                325                 330                 335     


Lys Ala Ile Gly Asn Cys Pro Ile Trp Val Lys Thr Pro Leu Lys Leu 
            340                 345                 350         


Ala Asn Gly Thr Lys Tyr Arg Pro Pro Gly Gly Gly Trp Glu Gly Met 
        355                 360                 365             


Ile Ala Gly Trp His Gly Tyr Thr Ser His Gly Ala His Gly Val Ala 
    370                 375                 380                 


Val Ala Ala Asp Leu Lys Ser Thr Gln Glu Ala Ile Asn Lys Ile Thr 
385                 390                 395                 400 


Lys Asn Leu Asn Ser Leu Ser Glu Leu Glu Val Lys Asn Leu Gln Arg 
                405                 410                 415     


Leu Ser Gly Ala Met Asp Glu Leu His Asn Glu Ile Leu Glu Leu Asp 
            420                 425                 430         


Glu Lys Val Asp Asp Leu Arg Ala Asp Thr Ile Ser Ser Gln Ile Glu 
        435                 440                 445             


Leu Ala Val Leu Leu Ser Asn Glu Gly Ile Ile Asn Ser Glu Asp Glu 
    450                 455                 460                 


His Leu Leu Ala Leu Glu Arg Lys Leu Lys Lys Met Leu Gly Pro Ser 
465                 470                 475                 480 


Ala Val Glu Ile Gly Asn Gly Cys Phe Glu Thr Lys His Lys Cys Asn 
                485                 490                 495     


Gln Thr Cys Leu Asp Arg Ile Ala Ala Gly Thr Phe Asp Ala Gly Glu 
            500                 505                 510         


Phe Ser Leu Pro Thr Phe Asp Ser Leu Asn Ile Thr Ala Ala Ser Leu 
        515                 520                 525             


Asn Asp Asp Gly Leu Asp Asn Tyr Gln Ile Leu Ala Ile Tyr Ser Thr 
    530                 535                 540                 


Val Ala Ser Ser Leu Val Leu Val Val Ser Leu Gly Ala Ile Ser Phe 
545                 550                 555                 560 


Trp Met Cys Ser Asn Gly Ser Leu Gln Cys Arg Ile Cys Ile 
                565                 570                 


<210>  162
<211>  1725
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PDI-B Wash-L201A DNA

<400>  162
atggcgaaaa acgttgcgat tttcggctta ttgttttctc ttcttgtgtt ggttccttct       60

cagatcttcg cggatcgaat ctgcactggg ataacatcgt caaactcacc acatgtcgtc      120

aaaactgcta ctcaagggga ggtcaatgtg accggtgtaa taccactgac aacaacaccc      180

accaaatctc attttgcaaa tctcaaagga acagaaacca gggggaaact atgcccaaaa      240

tgcctcaact gcacagatct ggatgtagcc ttgggcagac caaaatgcac agggaaaata      300

ccctctgcaa gggtttcaat actccatgaa gtcagacctg ttacatctgg gtgctttcct      360

ataatgcacg atagaacaaa aattagacag ctgcctaacc ttctccgagg atacgaacat      420

gtcaggttat caactcacaa cgttatcaat gcagaagatg caccaggaag accctacgag      480

attggaacct cagggtcttg ccctaacatt accaatggaa acggattctt cgcaacaatg      540

gcttgggccg tcccaaaaaa caaaacagca acaaatccat taacaataga agtaccatac      600

atttgtacag aaggagaaga ccaaattacc gtttgggggt tccactctga caacgagacc      660

caaatggcaa aggcctatgg ggactcaaag ccccagaagt tcacctcatc tgccaacgga      720

gtgaccacac attacgtttc acagattggt ggcttcccaa atcaaacaga agacggagga      780

ctaccacaaa gtggcagaat tgttgttgat tacatggtgc aaaaatctgg aaaaacagga      840

acaattacct atcaaagagg tattttattg cctcaaaagg tgtggtgcgc aagtggcagg      900

agcaaggtaa taaaaggatc cttgccttta attggagaag cagattgcct ccatgaaaaa      960

tacggtggat taaacaaaag caagccttac tacacagggg aacatgcaaa ggccatagga     1020

aattgcccaa tatgggtgaa aacacccttg aagctggcca atggaaccaa atatagacct     1080

cctggtggag gatgggaagg aatgattgca ggttggcacg gatacacatc ccatggggca     1140

catggagtag cggtggcagc agaccttaag agcactcaag aggccataaa caagataaca     1200

aaaaatctca actctttgag tgagctggaa gtaaagaatc ttcaaagact aagcggtgcc     1260

atggatgaac tccacaacga aatactagaa ctagatgaga aagtggatga tctcagagct     1320

gatacaataa gctcacaaat agaactcgca gtcctgcttt ccaatgaagg aataataaac     1380

agtgaagatg aacatctctt ggcgcttgaa agaaagctga agaaaatgct gggcccctct     1440

gctgtagaga tagggaatgg atgctttgaa accaaacaca agtgcaacca gacctgtctc     1500

gacagaatag ctgctggtac ctttgatgca ggagaatttt ctctccccac ctttgattca     1560

ctgaatatta ctgctgcatc tttaaatgac gatggattgg ataattacca gattttggcg     1620

atctattcaa ctgtcgccag ttcattggta ctggtagtct ccctgggggc aatcagtttc     1680

tggatgtgct ctaatgggtc tctacagtgt agaatatgta tttaa                     1725


<210>  163
<211>  574
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  PDI-B Wash-L201A AA

<400>  163

Met Ala Lys Asn Val Ala Ile Phe Gly Leu Leu Phe Ser Leu Leu Val 
1               5                   10                  15      


Leu Val Pro Ser Gln Ile Phe Ala Asp Arg Ile Cys Thr Gly Ile Thr 
            20                  25                  30          


Ser Ser Asn Ser Pro His Val Val Lys Thr Ala Thr Gln Gly Glu Val 
        35                  40                  45              


Asn Val Thr Gly Val Ile Pro Leu Thr Thr Thr Pro Thr Lys Ser His 
    50                  55                  60                  


Phe Ala Asn Leu Lys Gly Thr Glu Thr Arg Gly Lys Leu Cys Pro Lys 
65                  70                  75                  80  


Cys Leu Asn Cys Thr Asp Leu Asp Val Ala Leu Gly Arg Pro Lys Cys 
                85                  90                  95      


Thr Gly Lys Ile Pro Ser Ala Arg Val Ser Ile Leu His Glu Val Arg 
            100                 105                 110         


Pro Val Thr Ser Gly Cys Phe Pro Ile Met His Asp Arg Thr Lys Ile 
        115                 120                 125             


Arg Gln Leu Pro Asn Leu Leu Arg Gly Tyr Glu His Val Arg Leu Ser 
    130                 135                 140                 


Thr His Asn Val Ile Asn Ala Glu Asp Ala Pro Gly Arg Pro Tyr Glu 
145                 150                 155                 160 


Ile Gly Thr Ser Gly Ser Cys Pro Asn Ile Thr Asn Gly Asn Gly Phe 
                165                 170                 175     


Phe Ala Thr Met Ala Trp Ala Val Pro Lys Asn Lys Thr Ala Thr Asn 
            180                 185                 190         


Pro Leu Thr Ile Glu Val Pro Tyr Ile Cys Thr Glu Gly Glu Asp Gln 
        195                 200                 205             


Ile Thr Val Trp Gly Phe His Ser Asp Asn Glu Thr Gln Met Ala Lys 
    210                 215                 220                 


Ala Tyr Gly Asp Ser Lys Pro Gln Lys Phe Thr Ser Ser Ala Asn Gly 
225                 230                 235                 240 


Val Thr Thr His Tyr Val Ser Gln Ile Gly Gly Phe Pro Asn Gln Thr 
                245                 250                 255     


Glu Asp Gly Gly Leu Pro Gln Ser Gly Arg Ile Val Val Asp Tyr Met 
            260                 265                 270         


Val Gln Lys Ser Gly Lys Thr Gly Thr Ile Thr Tyr Gln Arg Gly Ile 
        275                 280                 285             


Leu Leu Pro Gln Lys Val Trp Cys Ala Ser Gly Arg Ser Lys Val Ile 
    290                 295                 300                 


Lys Gly Ser Leu Pro Leu Ile Gly Glu Ala Asp Cys Leu His Glu Lys 
305                 310                 315                 320 


Tyr Gly Gly Leu Asn Lys Ser Lys Pro Tyr Tyr Thr Gly Glu His Ala 
                325                 330                 335     


Lys Ala Ile Gly Asn Cys Pro Ile Trp Val Lys Thr Pro Leu Lys Leu 
            340                 345                 350         


Ala Asn Gly Thr Lys Tyr Arg Pro Pro Gly Gly Gly Trp Glu Gly Met 
        355                 360                 365             


Ile Ala Gly Trp His Gly Tyr Thr Ser His Gly Ala His Gly Val Ala 
    370                 375                 380                 


Val Ala Ala Asp Leu Lys Ser Thr Gln Glu Ala Ile Asn Lys Ile Thr 
385                 390                 395                 400 


Lys Asn Leu Asn Ser Leu Ser Glu Leu Glu Val Lys Asn Leu Gln Arg 
                405                 410                 415     


Leu Ser Gly Ala Met Asp Glu Leu His Asn Glu Ile Leu Glu Leu Asp 
            420                 425                 430         


Glu Lys Val Asp Asp Leu Arg Ala Asp Thr Ile Ser Ser Gln Ile Glu 
        435                 440                 445             


Leu Ala Val Leu Leu Ser Asn Glu Gly Ile Ile Asn Ser Glu Asp Glu 
    450                 455                 460                 


His Leu Leu Ala Leu Glu Arg Lys Leu Lys Lys Met Leu Gly Pro Ser 
465                 470                 475                 480 


Ala Val Glu Ile Gly Asn Gly Cys Phe Glu Thr Lys His Lys Cys Asn 
                485                 490                 495     


Gln Thr Cys Leu Asp Arg Ile Ala Ala Gly Thr Phe Asp Ala Gly Glu 
            500                 505                 510         


Phe Ser Leu Pro Thr Phe Asp Ser Leu Asn Ile Thr Ala Ala Ser Leu 
        515                 520                 525             


Asn Asp Asp Gly Leu Asp Asn Tyr Gln Ile Leu Ala Ile Tyr Ser Thr 
    530                 535                 540                 


Val Ala Ser Ser Leu Val Leu Val Val Ser Leu Gly Ala Ile Ser Phe 
545                 550                 555                 560 


Trp Met Cys Ser Asn Gly Ser Leu Gln Cys Arg Ile Cys Ile 
                565                 570                 


<210>  164
<211>  1725
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PDI-B Wash-L201W DNA

<400>  164
atggcgaaaa acgttgcgat tttcggctta ttgttttctc ttcttgtgtt ggttccttct       60

cagatcttcg cggatcgaat ctgcactggg ataacatcgt caaactcacc acatgtcgtc      120

aaaactgcta ctcaagggga ggtcaatgtg accggtgtaa taccactgac aacaacaccc      180

accaaatctc attttgcaaa tctcaaagga acagaaacca gggggaaact atgcccaaaa      240

tgcctcaact gcacagatct ggatgtagcc ttgggcagac caaaatgcac agggaaaata      300

ccctctgcaa gggtttcaat actccatgaa gtcagacctg ttacatctgg gtgctttcct      360

ataatgcacg atagaacaaa aattagacag ctgcctaacc ttctccgagg atacgaacat      420

gtcaggttat caactcacaa cgttatcaat gcagaagatg caccaggaag accctacgag      480

attggaacct cagggtcttg ccctaacatt accaatggaa acggattctt cgcaacaatg      540

gcttgggccg tcccaaaaaa caaaacagca acaaatccat taacaataga agtaccatac      600

atttgtacag aaggagaaga ccaaattacc gtttgggggt tccactctga caacgagacc      660

caaatggcaa agtggtatgg ggactcaaag ccccagaagt tcacctcatc tgccaacgga      720

gtgaccacac attacgtttc acagattggt ggcttcccaa atcaaacaga agacggagga      780

ctaccacaaa gtggcagaat tgttgttgat tacatggtgc aaaaatctgg aaaaacagga      840

acaattacct atcaaagagg tattttattg cctcaaaagg tgtggtgcgc aagtggcagg      900

agcaaggtaa taaaaggatc cttgccttta attggagaag cagattgcct ccatgaaaaa      960

tacggtggat taaacaaaag caagccttac tacacagggg aacatgcaaa ggccatagga     1020

aattgcccaa tatgggtgaa aacacccttg aagctggcca atggaaccaa atatagacct     1080

cctggtggag gatgggaagg aatgattgca ggttggcacg gatacacatc ccatggggca     1140

catggagtag cggtggcagc agaccttaag agcactcaag aggccataaa caagataaca     1200

aaaaatctca actctttgag tgagctggaa gtaaagaatc ttcaaagact aagcggtgcc     1260

atggatgaac tccacaacga aatactagaa ctagatgaga aagtggatga tctcagagct     1320

gatacaataa gctcacaaat agaactcgca gtcctgcttt ccaatgaagg aataataaac     1380

agtgaagatg aacatctctt ggcgcttgaa agaaagctga agaaaatgct gggcccctct     1440

gctgtagaga tagggaatgg atgctttgaa accaaacaca agtgcaacca gacctgtctc     1500

gacagaatag ctgctggtac ctttgatgca ggagaatttt ctctccccac ctttgattca     1560

ctgaatatta ctgctgcatc tttaaatgac gatggattgg ataattacca gattttggcg     1620

atctattcaa ctgtcgccag ttcattggta ctggtagtct ccctgggggc aatcagtttc     1680

tggatgtgct ctaatgggtc tctacagtgt agaatatgta tttaa                     1725


<210>  165
<211>  574
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  PDI-B Wash-L201W AA

<400>  165

Met Ala Lys Asn Val Ala Ile Phe Gly Leu Leu Phe Ser Leu Leu Val 
1               5                   10                  15      


Leu Val Pro Ser Gln Ile Phe Ala Asp Arg Ile Cys Thr Gly Ile Thr 
            20                  25                  30          


Ser Ser Asn Ser Pro His Val Val Lys Thr Ala Thr Gln Gly Glu Val 
        35                  40                  45              


Asn Val Thr Gly Val Ile Pro Leu Thr Thr Thr Pro Thr Lys Ser His 
    50                  55                  60                  


Phe Ala Asn Leu Lys Gly Thr Glu Thr Arg Gly Lys Leu Cys Pro Lys 
65                  70                  75                  80  


Cys Leu Asn Cys Thr Asp Leu Asp Val Ala Leu Gly Arg Pro Lys Cys 
                85                  90                  95      


Thr Gly Lys Ile Pro Ser Ala Arg Val Ser Ile Leu His Glu Val Arg 
            100                 105                 110         


Pro Val Thr Ser Gly Cys Phe Pro Ile Met His Asp Arg Thr Lys Ile 
        115                 120                 125             


Arg Gln Leu Pro Asn Leu Leu Arg Gly Tyr Glu His Val Arg Leu Ser 
    130                 135                 140                 


Thr His Asn Val Ile Asn Ala Glu Asp Ala Pro Gly Arg Pro Tyr Glu 
145                 150                 155                 160 


Ile Gly Thr Ser Gly Ser Cys Pro Asn Ile Thr Asn Gly Asn Gly Phe 
                165                 170                 175     


Phe Ala Thr Met Ala Trp Ala Val Pro Lys Asn Lys Thr Ala Thr Asn 
            180                 185                 190         


Pro Leu Thr Ile Glu Val Pro Tyr Ile Cys Thr Glu Gly Glu Asp Gln 
        195                 200                 205             


Ile Thr Val Trp Gly Phe His Ser Asp Asn Glu Thr Gln Met Ala Lys 
    210                 215                 220                 


Trp Tyr Gly Asp Ser Lys Pro Gln Lys Phe Thr Ser Ser Ala Asn Gly 
225                 230                 235                 240 


Val Thr Thr His Tyr Val Ser Gln Ile Gly Gly Phe Pro Asn Gln Thr 
                245                 250                 255     


Glu Asp Gly Gly Leu Pro Gln Ser Gly Arg Ile Val Val Asp Tyr Met 
            260                 265                 270         


Val Gln Lys Ser Gly Lys Thr Gly Thr Ile Thr Tyr Gln Arg Gly Ile 
        275                 280                 285             


Leu Leu Pro Gln Lys Val Trp Cys Ala Ser Gly Arg Ser Lys Val Ile 
    290                 295                 300                 


Lys Gly Ser Leu Pro Leu Ile Gly Glu Ala Asp Cys Leu His Glu Lys 
305                 310                 315                 320 


Tyr Gly Gly Leu Asn Lys Ser Lys Pro Tyr Tyr Thr Gly Glu His Ala 
                325                 330                 335     


Lys Ala Ile Gly Asn Cys Pro Ile Trp Val Lys Thr Pro Leu Lys Leu 
            340                 345                 350         


Ala Asn Gly Thr Lys Tyr Arg Pro Pro Gly Gly Gly Trp Glu Gly Met 
        355                 360                 365             


Ile Ala Gly Trp His Gly Tyr Thr Ser His Gly Ala His Gly Val Ala 
    370                 375                 380                 


Val Ala Ala Asp Leu Lys Ser Thr Gln Glu Ala Ile Asn Lys Ile Thr 
385                 390                 395                 400 


Lys Asn Leu Asn Ser Leu Ser Glu Leu Glu Val Lys Asn Leu Gln Arg 
                405                 410                 415     


Leu Ser Gly Ala Met Asp Glu Leu His Asn Glu Ile Leu Glu Leu Asp 
            420                 425                 430         


Glu Lys Val Asp Asp Leu Arg Ala Asp Thr Ile Ser Ser Gln Ile Glu 
        435                 440                 445             


Leu Ala Val Leu Leu Ser Asn Glu Gly Ile Ile Asn Ser Glu Asp Glu 
    450                 455                 460                 


His Leu Leu Ala Leu Glu Arg Lys Leu Lys Lys Met Leu Gly Pro Ser 
465                 470                 475                 480 


Ala Val Glu Ile Gly Asn Gly Cys Phe Glu Thr Lys His Lys Cys Asn 
                485                 490                 495     


Gln Thr Cys Leu Asp Arg Ile Ala Ala Gly Thr Phe Asp Ala Gly Glu 
            500                 505                 510         


Phe Ser Leu Pro Thr Phe Asp Ser Leu Asn Ile Thr Ala Ala Ser Leu 
        515                 520                 525             


Asn Asp Asp Gly Leu Asp Asn Tyr Gln Ile Leu Ala Ile Tyr Ser Thr 
    530                 535                 540                 


Val Ala Ser Ser Leu Val Leu Val Val Ser Leu Gly Ala Ile Ser Phe 
545                 550                 555                 560 


Trp Met Cys Ser Asn Gly Ser Leu Gln Cys Arg Ile Cys Ile 
                565                 570                 


<210>  166

<400>  166
000

<210>  167

<400>  167
000

<210>  168

<400>  168
000

<210>  169

<400>  169
000

<210>  170

<400>  170
000

<210>  171

<400>  171
000

<210>  172

<400>  172
000

<210>  173

<400>  173
000

<210>  174

<400>  174
000

<210>  175

<400>  175
000

<210>  176

<400>  176
000

<210>  177

<400>  177
000

<210>  178

<400>  178
000

<210>  179

<400>  179
000

<210>  180
<211>  1725
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PDI-B Vic DNA

<400>  180
atggcgaaaa acgttgcgat tttcggctta ttgttttctc ttcttgtgtt ggttccttct       60

cagatcttcg cggatcgaat ctgcactggg ataacatcgt caaactcacc acatgtcgtc      120

aaaactgcta ctcaagggga ggtcaatgtg accggtgtaa taccactgac aacaacaccc      180

accaaatctc attttgcaaa tctcaaagga acagaaacca gggggaaact atgcccaaaa      240

tgcctcaact gcacagatct ggatgtagcc ttgagcagac caaaatgcac agggaaaata      300

ccctctgcaa gggtttcaat actccatgaa gtcagacctg ttacatctgg gtgctttcct      360

ataatgcacg atagaacaaa aattagacag ctgcctaacc ttctccgagg atacgaacat      420

gtcaggttat caactcacaa cgttatcaat gcagaagatg caccaggagg accctacgag      480

attggaacct cagggtcttg ccctaacatt accaatggaa acggattctt cgcaacaatg      540

gcttgggccg tcccaaaaaa caaaacagca acaaatccat taacaataga agtaccatac      600

atttgtacag aaggagaaga ccaaattacc gtttgggggt tccactctga caacgagacc      660

caaatggcaa agctctatgg ggactcaaag ccccagaagt tcacctcatc tgccaacgga      720

gtgaccacac attacgtttc acagattggt ggcttcccaa atcaaacaga agacggagga      780

ctaccacaaa gtggcagaat tgttgttgat tacatggtgc aaaaatctgg aaaaacagga      840

acaattacct atcaaagagg tattttattg cctcaaaagg tgtggtgcgc aagtggcagg      900

agcaaggtaa taaaaggatc cttgccttta attggagaag cagattgcct ccatgaaaaa      960

tacggtggat taaacaaaag caagccttac tacacagggg aacatgcaaa ggccatagga     1020

aattgcccaa tatgggtgaa aacacccttg aagctggcca atggaaccaa atatagacct     1080

cctggtggag gatgggaagg aatgattgca ggttggcacg gatacacatc ccatggggca     1140

catggagtag cggtggcagc agaccttaag agcactcaag aggccataaa caagataaca     1200

aaaaatctca actctttgag tgagctggaa gtaaagaatc ttcaaagact aagcggtgcc     1260

atggatgaac tccacaacga aatactagaa ctagatgaga aagtggatga tctcagagct     1320

gatacaataa gctcacaaat agaactcgca gtcctgcttt ccaatgaagg aataataaac     1380

agtgaagatg aacatctctt ggcgcttgaa agaaagctga agaaaatgct gggcccctct     1440

gctgtagaga tagggaatgg atgctttgaa accaaacaca agtgcaacca gacctgtctc     1500

gacagaatag ctgctggtac ctttgatgca ggagaatttt ctctccccac ctttgattca     1560

ctgaatatta ctgctgcatc tttaaatgac gatggattgg ataattacca gattttggcg     1620

atctattcaa ctgtcgccag ttcattggta ctggtagtct ccctgggggc aatcagtttc     1680

tggatgtgct ctaatgggtc tctacagtgt agaatatgta tttaa                     1725


<210>  181
<211>  574
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  PDI-B Vic AA

<400>  181

Met Ala Lys Asn Val Ala Ile Phe Gly Leu Leu Phe Ser Leu Leu Val 
1               5                   10                  15      


Leu Val Pro Ser Gln Ile Phe Ala Asp Arg Ile Cys Thr Gly Ile Thr 
            20                  25                  30          


Ser Ser Asn Ser Pro His Val Val Lys Thr Ala Thr Gln Gly Glu Val 
        35                  40                  45              


Asn Val Thr Gly Val Ile Pro Leu Thr Thr Thr Pro Thr Lys Ser His 
    50                  55                  60                  


Phe Ala Asn Leu Lys Gly Thr Glu Thr Arg Gly Lys Leu Cys Pro Lys 
65                  70                  75                  80  


Cys Leu Asn Cys Thr Asp Leu Asp Val Ala Leu Ser Arg Pro Lys Cys 
                85                  90                  95      


Thr Gly Lys Ile Pro Ser Ala Arg Val Ser Ile Leu His Glu Val Arg 
            100                 105                 110         


Pro Val Thr Ser Gly Cys Phe Pro Ile Met His Asp Arg Thr Lys Ile 
        115                 120                 125             


Arg Gln Leu Pro Asn Leu Leu Arg Gly Tyr Glu His Val Arg Leu Ser 
    130                 135                 140                 


Thr His Asn Val Ile Asn Ala Glu Asp Ala Pro Gly Gly Pro Tyr Glu 
145                 150                 155                 160 


Ile Gly Thr Ser Gly Ser Cys Pro Asn Ile Thr Asn Gly Asn Gly Phe 
                165                 170                 175     


Phe Ala Thr Met Ala Trp Ala Val Pro Lys Asn Lys Thr Ala Thr Asn 
            180                 185                 190         


Pro Leu Thr Ile Glu Val Pro Tyr Ile Cys Thr Glu Gly Glu Asp Gln 
        195                 200                 205             


Ile Thr Val Trp Gly Phe His Ser Asp Asn Glu Thr Gln Met Ala Lys 
    210                 215                 220                 


Leu Tyr Gly Asp Ser Lys Pro Gln Lys Phe Thr Ser Ser Ala Asn Gly 
225                 230                 235                 240 


Val Thr Thr His Tyr Val Ser Gln Ile Gly Gly Phe Pro Asn Gln Thr 
                245                 250                 255     


Glu Asp Gly Gly Leu Pro Gln Ser Gly Arg Ile Val Val Asp Tyr Met 
            260                 265                 270         


Val Gln Lys Ser Gly Lys Thr Gly Thr Ile Thr Tyr Gln Arg Gly Ile 
        275                 280                 285             


Leu Leu Pro Gln Lys Val Trp Cys Ala Ser Gly Arg Ser Lys Val Ile 
    290                 295                 300                 


Lys Gly Ser Leu Pro Leu Ile Gly Glu Ala Asp Cys Leu His Glu Lys 
305                 310                 315                 320 


Tyr Gly Gly Leu Asn Lys Ser Lys Pro Tyr Tyr Thr Gly Glu His Ala 
                325                 330                 335     


Lys Ala Ile Gly Asn Cys Pro Ile Trp Val Lys Thr Pro Leu Lys Leu 
            340                 345                 350         


Ala Asn Gly Thr Lys Tyr Arg Pro Pro Gly Gly Gly Trp Glu Gly Met 
        355                 360                 365             


Ile Ala Gly Trp His Gly Tyr Thr Ser His Gly Ala His Gly Val Ala 
    370                 375                 380                 


Val Ala Ala Asp Leu Lys Ser Thr Gln Glu Ala Ile Asn Lys Ile Thr 
385                 390                 395                 400 


Lys Asn Leu Asn Ser Leu Ser Glu Leu Glu Val Lys Asn Leu Gln Arg 
                405                 410                 415     


Leu Ser Gly Ala Met Asp Glu Leu His Asn Glu Ile Leu Glu Leu Asp 
            420                 425                 430         


Glu Lys Val Asp Asp Leu Arg Ala Asp Thr Ile Ser Ser Gln Ile Glu 
        435                 440                 445             


Leu Ala Val Leu Leu Ser Asn Glu Gly Ile Ile Asn Ser Glu Asp Glu 
    450                 455                 460                 


His Leu Leu Ala Leu Glu Arg Lys Leu Lys Lys Met Leu Gly Pro Ser 
465                 470                 475                 480 


Ala Val Glu Ile Gly Asn Gly Cys Phe Glu Thr Lys His Lys Cys Asn 
                485                 490                 495     


Gln Thr Cys Leu Asp Arg Ile Ala Ala Gly Thr Phe Asp Ala Gly Glu 
            500                 505                 510         


Phe Ser Leu Pro Thr Phe Asp Ser Leu Asn Ile Thr Ala Ala Ser Leu 
        515                 520                 525             


Asn Asp Asp Gly Leu Asp Asn Tyr Gln Ile Leu Ala Ile Tyr Ser Thr 
    530                 535                 540                 


Val Ala Ser Ser Leu Val Leu Val Val Ser Leu Gly Ala Ile Ser Phe 
545                 550                 555                 560 


Trp Met Cys Ser Asn Gly Ser Leu Gln Cys Arg Ile Cys Ile 
                565                 570                 


<210>  182
<211>  1725
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PDI-B Vic-G138A DNA

<400>  182
atggcgaaaa acgttgcgat tttcggctta ttgttttctc ttcttgtgtt ggttccttct       60

cagatcttcg cggatcgaat ctgcactggg ataacatcgt caaactcacc acatgtcgtc      120

aaaactgcta ctcaagggga ggtcaatgtg accggtgtaa taccactgac aacaacaccc      180

accaaatctc attttgcaaa tctcaaagga acagaaacca gggggaaact atgcccaaaa      240

tgcctcaact gcacagatct ggatgtagcc ttgagcagac caaaatgcac agggaaaata      300

ccctctgcaa gggtttcaat actccatgaa gtcagacctg ttacatctgg gtgctttcct      360

ataatgcacg atagaacaaa aattagacag ctgcctaacc ttctccgagg atacgaacat      420

gtcaggttat caactcacaa cgttatcaat gcagaagatg caccaggagg accctacgag      480

attgccacct cagggtcttg ccctaacatt accaatggaa acggattctt cgcaacaatg      540

gcttgggccg tcccaaaaaa caaaacagca acaaatccat taacaataga agtaccatac      600

atttgtacag aaggagaaga ccaaattacc gtttgggggt tccactctga caacgagacc      660

caaatggcaa agctctatgg ggactcaaag ccccagaagt tcacctcatc tgccaacgga      720

gtgaccacac attacgtttc acagattggt ggcttcccaa atcaaacaga agacggagga      780

ctaccacaaa gtggcagaat tgttgttgat tacatggtgc aaaaatctgg aaaaacagga      840

acaattacct atcaaagagg tattttattg cctcaaaagg tgtggtgcgc aagtggcagg      900

agcaaggtaa taaaaggatc cttgccttta attggagaag cagattgcct ccatgaaaaa      960

tacggtggat taaacaaaag caagccttac tacacagggg aacatgcaaa ggccatagga     1020

aattgcccaa tatgggtgaa aacacccttg aagctggcca atggaaccaa atatagacct     1080

cctggtggag gatgggaagg aatgattgca ggttggcacg gatacacatc ccatggggca     1140

catggagtag cggtggcagc agaccttaag agcactcaag aggccataaa caagataaca     1200

aaaaatctca actctttgag tgagctggaa gtaaagaatc ttcaaagact aagcggtgcc     1260

atggatgaac tccacaacga aatactagaa ctagatgaga aagtggatga tctcagagct     1320

gatacaataa gctcacaaat agaactcgca gtcctgcttt ccaatgaagg aataataaac     1380

agtgaagatg aacatctctt ggcgcttgaa agaaagctga agaaaatgct gggcccctct     1440

gctgtagaga tagggaatgg atgctttgaa accaaacaca agtgcaacca gacctgtctc     1500

gacagaatag ctgctggtac ctttgatgca ggagaatttt ctctccccac ctttgattca     1560

ctgaatatta ctgctgcatc tttaaatgac gatggattgg ataattacca gattttggcg     1620

atctattcaa ctgtcgccag ttcattggta ctggtagtct ccctgggggc aatcagtttc     1680

tggatgtgct ctaatgggtc tctacagtgt agaatatgta tttaa                     1725


<210>  183
<211>  574
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  PDI-B Vic-G138A AA

<400>  183

Met Ala Lys Asn Val Ala Ile Phe Gly Leu Leu Phe Ser Leu Leu Val 
1               5                   10                  15      


Leu Val Pro Ser Gln Ile Phe Ala Asp Arg Ile Cys Thr Gly Ile Thr 
            20                  25                  30          


Ser Ser Asn Ser Pro His Val Val Lys Thr Ala Thr Gln Gly Glu Val 
        35                  40                  45              


Asn Val Thr Gly Val Ile Pro Leu Thr Thr Thr Pro Thr Lys Ser His 
    50                  55                  60                  


Phe Ala Asn Leu Lys Gly Thr Glu Thr Arg Gly Lys Leu Cys Pro Lys 
65                  70                  75                  80  


Cys Leu Asn Cys Thr Asp Leu Asp Val Ala Leu Ser Arg Pro Lys Cys 
                85                  90                  95      


Thr Gly Lys Ile Pro Ser Ala Arg Val Ser Ile Leu His Glu Val Arg 
            100                 105                 110         


Pro Val Thr Ser Gly Cys Phe Pro Ile Met His Asp Arg Thr Lys Ile 
        115                 120                 125             


Arg Gln Leu Pro Asn Leu Leu Arg Gly Tyr Glu His Val Arg Leu Ser 
    130                 135                 140                 


Thr His Asn Val Ile Asn Ala Glu Asp Ala Pro Gly Gly Pro Tyr Glu 
145                 150                 155                 160 


Ile Ala Thr Ser Gly Ser Cys Pro Asn Ile Thr Asn Gly Asn Gly Phe 
                165                 170                 175     


Phe Ala Thr Met Ala Trp Ala Val Pro Lys Asn Lys Thr Ala Thr Asn 
            180                 185                 190         


Pro Leu Thr Ile Glu Val Pro Tyr Ile Cys Thr Glu Gly Glu Asp Gln 
        195                 200                 205             


Ile Thr Val Trp Gly Phe His Ser Asp Asn Glu Thr Gln Met Ala Lys 
    210                 215                 220                 


Leu Tyr Gly Asp Ser Lys Pro Gln Lys Phe Thr Ser Ser Ala Asn Gly 
225                 230                 235                 240 


Val Thr Thr His Tyr Val Ser Gln Ile Gly Gly Phe Pro Asn Gln Thr 
                245                 250                 255     


Glu Asp Gly Gly Leu Pro Gln Ser Gly Arg Ile Val Val Asp Tyr Met 
            260                 265                 270         


Val Gln Lys Ser Gly Lys Thr Gly Thr Ile Thr Tyr Gln Arg Gly Ile 
        275                 280                 285             


Leu Leu Pro Gln Lys Val Trp Cys Ala Ser Gly Arg Ser Lys Val Ile 
    290                 295                 300                 


Lys Gly Ser Leu Pro Leu Ile Gly Glu Ala Asp Cys Leu His Glu Lys 
305                 310                 315                 320 


Tyr Gly Gly Leu Asn Lys Ser Lys Pro Tyr Tyr Thr Gly Glu His Ala 
                325                 330                 335     


Lys Ala Ile Gly Asn Cys Pro Ile Trp Val Lys Thr Pro Leu Lys Leu 
            340                 345                 350         


Ala Asn Gly Thr Lys Tyr Arg Pro Pro Gly Gly Gly Trp Glu Gly Met 
        355                 360                 365             


Ile Ala Gly Trp His Gly Tyr Thr Ser His Gly Ala His Gly Val Ala 
    370                 375                 380                 


Val Ala Ala Asp Leu Lys Ser Thr Gln Glu Ala Ile Asn Lys Ile Thr 
385                 390                 395                 400 


Lys Asn Leu Asn Ser Leu Ser Glu Leu Glu Val Lys Asn Leu Gln Arg 
                405                 410                 415     


Leu Ser Gly Ala Met Asp Glu Leu His Asn Glu Ile Leu Glu Leu Asp 
            420                 425                 430         


Glu Lys Val Asp Asp Leu Arg Ala Asp Thr Ile Ser Ser Gln Ile Glu 
        435                 440                 445             


Leu Ala Val Leu Leu Ser Asn Glu Gly Ile Ile Asn Ser Glu Asp Glu 
    450                 455                 460                 


His Leu Leu Ala Leu Glu Arg Lys Leu Lys Lys Met Leu Gly Pro Ser 
465                 470                 475                 480 


Ala Val Glu Ile Gly Asn Gly Cys Phe Glu Thr Lys His Lys Cys Asn 
                485                 490                 495     


Gln Thr Cys Leu Asp Arg Ile Ala Ala Gly Thr Phe Asp Ala Gly Glu 
            500                 505                 510         


Phe Ser Leu Pro Thr Phe Asp Ser Leu Asn Ile Thr Ala Ala Ser Leu 
        515                 520                 525             


Asn Asp Asp Gly Leu Asp Asn Tyr Gln Ile Leu Ala Ile Tyr Ser Thr 
    530                 535                 540                 


Val Ala Ser Ser Leu Val Leu Val Val Ser Leu Gly Ala Ile Ser Phe 
545                 550                 555                 560 


Trp Met Cys Ser Asn Gly Ser Leu Gln Cys Arg Ile Cys Ile 
                565                 570                 


<210>  184
<211>  1725
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PDI-B Vic-S140A DNA

<400>  184
atggcgaaaa acgttgcgat tttcggctta ttgttttctc ttcttgtgtt ggttccttct       60

cagatcttcg cggatcgaat ctgcactggg ataacatcgt caaactcacc acatgtcgtc      120

aaaactgcta ctcaagggga ggtcaatgtg accggtgtaa taccactgac aacaacaccc      180

accaaatctc attttgcaaa tctcaaagga acagaaacca gggggaaact atgcccaaaa      240

tgcctcaact gcacagatct ggatgtagcc ttgagcagac caaaatgcac agggaaaata      300

ccctctgcaa gggtttcaat actccatgaa gtcagacctg ttacatctgg gtgctttcct      360

ataatgcacg atagaacaaa aattagacag ctgcctaacc ttctccgagg atacgaacat      420

gtcaggttat caactcacaa cgttatcaat gcagaagatg caccaggagg accctacgag      480

attggaaccg ccgggtcttg ccctaacatt accaatggaa acggattctt cgcaacaatg      540

gcttgggccg tcccaaaaaa caaaacagca acaaatccat taacaataga agtaccatac      600

atttgtacag aaggagaaga ccaaattacc gtttgggggt tccactctga caacgagacc      660

caaatggcaa agctctatgg ggactcaaag ccccagaagt tcacctcatc tgccaacgga      720

gtgaccacac attacgtttc acagattggt ggcttcccaa atcaaacaga agacggagga      780

ctaccacaaa gtggcagaat tgttgttgat tacatggtgc aaaaatctgg aaaaacagga      840

acaattacct atcaaagagg tattttattg cctcaaaagg tgtggtgcgc aagtggcagg      900

agcaaggtaa taaaaggatc cttgccttta attggagaag cagattgcct ccatgaaaaa      960

tacggtggat taaacaaaag caagccttac tacacagggg aacatgcaaa ggccatagga     1020

aattgcccaa tatgggtgaa aacacccttg aagctggcca atggaaccaa atatagacct     1080

cctggtggag gatgggaagg aatgattgca ggttggcacg gatacacatc ccatggggca     1140

catggagtag cggtggcagc agaccttaag agcactcaag aggccataaa caagataaca     1200

aaaaatctca actctttgag tgagctggaa gtaaagaatc ttcaaagact aagcggtgcc     1260

atggatgaac tccacaacga aatactagaa ctagatgaga aagtggatga tctcagagct     1320

gatacaataa gctcacaaat agaactcgca gtcctgcttt ccaatgaagg aataataaac     1380

agtgaagatg aacatctctt ggcgcttgaa agaaagctga agaaaatgct gggcccctct     1440

gctgtagaga tagggaatgg atgctttgaa accaaacaca agtgcaacca gacctgtctc     1500

gacagaatag ctgctggtac ctttgatgca ggagaatttt ctctccccac ctttgattca     1560

ctgaatatta ctgctgcatc tttaaatgac gatggattgg ataattacca gattttggcg     1620

atctattcaa ctgtcgccag ttcattggta ctggtagtct ccctgggggc aatcagtttc     1680

tggatgtgct ctaatgggtc tctacagtgt agaatatgta tttaa                     1725


<210>  185
<211>  574
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  PDI-B Vic-S140A AA

<400>  185

Met Ala Lys Asn Val Ala Ile Phe Gly Leu Leu Phe Ser Leu Leu Val 
1               5                   10                  15      


Leu Val Pro Ser Gln Ile Phe Ala Asp Arg Ile Cys Thr Gly Ile Thr 
            20                  25                  30          


Ser Ser Asn Ser Pro His Val Val Lys Thr Ala Thr Gln Gly Glu Val 
        35                  40                  45              


Asn Val Thr Gly Val Ile Pro Leu Thr Thr Thr Pro Thr Lys Ser His 
    50                  55                  60                  


Phe Ala Asn Leu Lys Gly Thr Glu Thr Arg Gly Lys Leu Cys Pro Lys 
65                  70                  75                  80  


Cys Leu Asn Cys Thr Asp Leu Asp Val Ala Leu Ser Arg Pro Lys Cys 
                85                  90                  95      


Thr Gly Lys Ile Pro Ser Ala Arg Val Ser Ile Leu His Glu Val Arg 
            100                 105                 110         


Pro Val Thr Ser Gly Cys Phe Pro Ile Met His Asp Arg Thr Lys Ile 
        115                 120                 125             


Arg Gln Leu Pro Asn Leu Leu Arg Gly Tyr Glu His Val Arg Leu Ser 
    130                 135                 140                 


Thr His Asn Val Ile Asn Ala Glu Asp Ala Pro Gly Gly Pro Tyr Glu 
145                 150                 155                 160 


Ile Gly Thr Ala Gly Ser Cys Pro Asn Ile Thr Asn Gly Asn Gly Phe 
                165                 170                 175     


Phe Ala Thr Met Ala Trp Ala Val Pro Lys Asn Lys Thr Ala Thr Asn 
            180                 185                 190         


Pro Leu Thr Ile Glu Val Pro Tyr Ile Cys Thr Glu Gly Glu Asp Gln 
        195                 200                 205             


Ile Thr Val Trp Gly Phe His Ser Asp Asn Glu Thr Gln Met Ala Lys 
    210                 215                 220                 


Leu Tyr Gly Asp Ser Lys Pro Gln Lys Phe Thr Ser Ser Ala Asn Gly 
225                 230                 235                 240 


Val Thr Thr His Tyr Val Ser Gln Ile Gly Gly Phe Pro Asn Gln Thr 
                245                 250                 255     


Glu Asp Gly Gly Leu Pro Gln Ser Gly Arg Ile Val Val Asp Tyr Met 
            260                 265                 270         


Val Gln Lys Ser Gly Lys Thr Gly Thr Ile Thr Tyr Gln Arg Gly Ile 
        275                 280                 285             


Leu Leu Pro Gln Lys Val Trp Cys Ala Ser Gly Arg Ser Lys Val Ile 
    290                 295                 300                 


Lys Gly Ser Leu Pro Leu Ile Gly Glu Ala Asp Cys Leu His Glu Lys 
305                 310                 315                 320 


Tyr Gly Gly Leu Asn Lys Ser Lys Pro Tyr Tyr Thr Gly Glu His Ala 
                325                 330                 335     


Lys Ala Ile Gly Asn Cys Pro Ile Trp Val Lys Thr Pro Leu Lys Leu 
            340                 345                 350         


Ala Asn Gly Thr Lys Tyr Arg Pro Pro Gly Gly Gly Trp Glu Gly Met 
        355                 360                 365             


Ile Ala Gly Trp His Gly Tyr Thr Ser His Gly Ala His Gly Val Ala 
    370                 375                 380                 


Val Ala Ala Asp Leu Lys Ser Thr Gln Glu Ala Ile Asn Lys Ile Thr 
385                 390                 395                 400 


Lys Asn Leu Asn Ser Leu Ser Glu Leu Glu Val Lys Asn Leu Gln Arg 
                405                 410                 415     


Leu Ser Gly Ala Met Asp Glu Leu His Asn Glu Ile Leu Glu Leu Asp 
            420                 425                 430         


Glu Lys Val Asp Asp Leu Arg Ala Asp Thr Ile Ser Ser Gln Ile Glu 
        435                 440                 445             


Leu Ala Val Leu Leu Ser Asn Glu Gly Ile Ile Asn Ser Glu Asp Glu 
    450                 455                 460                 


His Leu Leu Ala Leu Glu Arg Lys Leu Lys Lys Met Leu Gly Pro Ser 
465                 470                 475                 480 


Ala Val Glu Ile Gly Asn Gly Cys Phe Glu Thr Lys His Lys Cys Asn 
                485                 490                 495     


Gln Thr Cys Leu Asp Arg Ile Ala Ala Gly Thr Phe Asp Ala Gly Glu 
            500                 505                 510         


Phe Ser Leu Pro Thr Phe Asp Ser Leu Asn Ile Thr Ala Ala Ser Leu 
        515                 520                 525             


Asn Asp Asp Gly Leu Asp Asn Tyr Gln Ile Leu Ala Ile Tyr Ser Thr 
    530                 535                 540                 


Val Ala Ser Ser Leu Val Leu Val Val Ser Leu Gly Ala Ile Ser Phe 
545                 550                 555                 560 


Trp Met Cys Ser Asn Gly Ser Leu Gln Cys Arg Ile Cys Ile 
                565                 570                 


<210>  186
<211>  1725
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PDI-B Vic-S142A DNA

<400>  186
atggcgaaaa acgttgcgat tttcggctta ttgttttctc ttcttgtgtt ggttccttct       60

cagatcttcg cggatcgaat ctgcactggg ataacatcgt caaactcacc acatgtcgtc      120

aaaactgcta ctcaagggga ggtcaatgtg accggtgtaa taccactgac aacaacaccc      180

accaaatctc attttgcaaa tctcaaagga acagaaacca gggggaaact atgcccaaaa      240

tgcctcaact gcacagatct ggatgtagcc ttgagcagac caaaatgcac agggaaaata      300

ccctctgcaa gggtttcaat actccatgaa gtcagacctg ttacatctgg gtgctttcct      360

ataatgcacg atagaacaaa aattagacag ctgcctaacc ttctccgagg atacgaacat      420

gtcaggttat caactcacaa cgttatcaat gcagaagatg caccaggagg accctacgag      480

attggaacct caggggcctg ccctaacatt accaatggaa acggattctt cgcaacaatg      540

gcttgggccg tcccaaaaaa caaaacagca acaaatccat taacaataga agtaccatac      600

atttgtacag aaggagaaga ccaaattacc gtttgggggt tccactctga caacgagacc      660

caaatggcaa agctctatgg ggactcaaag ccccagaagt tcacctcatc tgccaacgga      720

gtgaccacac attacgtttc acagattggt ggcttcccaa atcaaacaga agacggagga      780

ctaccacaaa gtggcagaat tgttgttgat tacatggtgc aaaaatctgg aaaaacagga      840

acaattacct atcaaagagg tattttattg cctcaaaagg tgtggtgcgc aagtggcagg      900

agcaaggtaa taaaaggatc cttgccttta attggagaag cagattgcct ccatgaaaaa      960

tacggtggat taaacaaaag caagccttac tacacagggg aacatgcaaa ggccatagga     1020

aattgcccaa tatgggtgaa aacacccttg aagctggcca atggaaccaa atatagacct     1080

cctggtggag gatgggaagg aatgattgca ggttggcacg gatacacatc ccatggggca     1140

catggagtag cggtggcagc agaccttaag agcactcaag aggccataaa caagataaca     1200

aaaaatctca actctttgag tgagctggaa gtaaagaatc ttcaaagact aagcggtgcc     1260

atggatgaac tccacaacga aatactagaa ctagatgaga aagtggatga tctcagagct     1320

gatacaataa gctcacaaat agaactcgca gtcctgcttt ccaatgaagg aataataaac     1380

agtgaagatg aacatctctt ggcgcttgaa agaaagctga agaaaatgct gggcccctct     1440

gctgtagaga tagggaatgg atgctttgaa accaaacaca agtgcaacca gacctgtctc     1500

gacagaatag ctgctggtac ctttgatgca ggagaatttt ctctccccac ctttgattca     1560

ctgaatatta ctgctgcatc tttaaatgac gatggattgg ataattacca gattttggcg     1620

atctattcaa ctgtcgccag ttcattggta ctggtagtct ccctgggggc aatcagtttc     1680

tggatgtgct ctaatgggtc tctacagtgt agaatatgta tttaa                     1725


<210>  187
<211>  574
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  PDI-B Vic-S142A AA

<400>  187

Met Ala Lys Asn Val Ala Ile Phe Gly Leu Leu Phe Ser Leu Leu Val 
1               5                   10                  15      


Leu Val Pro Ser Gln Ile Phe Ala Asp Arg Ile Cys Thr Gly Ile Thr 
            20                  25                  30          


Ser Ser Asn Ser Pro His Val Val Lys Thr Ala Thr Gln Gly Glu Val 
        35                  40                  45              


Asn Val Thr Gly Val Ile Pro Leu Thr Thr Thr Pro Thr Lys Ser His 
    50                  55                  60                  


Phe Ala Asn Leu Lys Gly Thr Glu Thr Arg Gly Lys Leu Cys Pro Lys 
65                  70                  75                  80  


Cys Leu Asn Cys Thr Asp Leu Asp Val Ala Leu Ser Arg Pro Lys Cys 
                85                  90                  95      


Thr Gly Lys Ile Pro Ser Ala Arg Val Ser Ile Leu His Glu Val Arg 
            100                 105                 110         


Pro Val Thr Ser Gly Cys Phe Pro Ile Met His Asp Arg Thr Lys Ile 
        115                 120                 125             


Arg Gln Leu Pro Asn Leu Leu Arg Gly Tyr Glu His Val Arg Leu Ser 
    130                 135                 140                 


Thr His Asn Val Ile Asn Ala Glu Asp Ala Pro Gly Gly Pro Tyr Glu 
145                 150                 155                 160 


Ile Gly Thr Ser Gly Ala Cys Pro Asn Ile Thr Asn Gly Asn Gly Phe 
                165                 170                 175     


Phe Ala Thr Met Ala Trp Ala Val Pro Lys Asn Lys Thr Ala Thr Asn 
            180                 185                 190         


Pro Leu Thr Ile Glu Val Pro Tyr Ile Cys Thr Glu Gly Glu Asp Gln 
        195                 200                 205             


Ile Thr Val Trp Gly Phe His Ser Asp Asn Glu Thr Gln Met Ala Lys 
    210                 215                 220                 


Leu Tyr Gly Asp Ser Lys Pro Gln Lys Phe Thr Ser Ser Ala Asn Gly 
225                 230                 235                 240 


Val Thr Thr His Tyr Val Ser Gln Ile Gly Gly Phe Pro Asn Gln Thr 
                245                 250                 255     


Glu Asp Gly Gly Leu Pro Gln Ser Gly Arg Ile Val Val Asp Tyr Met 
            260                 265                 270         


Val Gln Lys Ser Gly Lys Thr Gly Thr Ile Thr Tyr Gln Arg Gly Ile 
        275                 280                 285             


Leu Leu Pro Gln Lys Val Trp Cys Ala Ser Gly Arg Ser Lys Val Ile 
    290                 295                 300                 


Lys Gly Ser Leu Pro Leu Ile Gly Glu Ala Asp Cys Leu His Glu Lys 
305                 310                 315                 320 


Tyr Gly Gly Leu Asn Lys Ser Lys Pro Tyr Tyr Thr Gly Glu His Ala 
                325                 330                 335     


Lys Ala Ile Gly Asn Cys Pro Ile Trp Val Lys Thr Pro Leu Lys Leu 
            340                 345                 350         


Ala Asn Gly Thr Lys Tyr Arg Pro Pro Gly Gly Gly Trp Glu Gly Met 
        355                 360                 365             


Ile Ala Gly Trp His Gly Tyr Thr Ser His Gly Ala His Gly Val Ala 
    370                 375                 380                 


Val Ala Ala Asp Leu Lys Ser Thr Gln Glu Ala Ile Asn Lys Ile Thr 
385                 390                 395                 400 


Lys Asn Leu Asn Ser Leu Ser Glu Leu Glu Val Lys Asn Leu Gln Arg 
                405                 410                 415     


Leu Ser Gly Ala Met Asp Glu Leu His Asn Glu Ile Leu Glu Leu Asp 
            420                 425                 430         


Glu Lys Val Asp Asp Leu Arg Ala Asp Thr Ile Ser Ser Gln Ile Glu 
        435                 440                 445             


Leu Ala Val Leu Leu Ser Asn Glu Gly Ile Ile Asn Ser Glu Asp Glu 
    450                 455                 460                 


His Leu Leu Ala Leu Glu Arg Lys Leu Lys Lys Met Leu Gly Pro Ser 
465                 470                 475                 480 


Ala Val Glu Ile Gly Asn Gly Cys Phe Glu Thr Lys His Lys Cys Asn 
                485                 490                 495     


Gln Thr Cys Leu Asp Arg Ile Ala Ala Gly Thr Phe Asp Ala Gly Glu 
            500                 505                 510         


Phe Ser Leu Pro Thr Phe Asp Ser Leu Asn Ile Thr Ala Ala Ser Leu 
        515                 520                 525             


Asn Asp Asp Gly Leu Asp Asn Tyr Gln Ile Leu Ala Ile Tyr Ser Thr 
    530                 535                 540                 


Val Ala Ser Ser Leu Val Leu Val Val Ser Leu Gly Ala Ile Ser Phe 
545                 550                 555                 560 


Trp Met Cys Ser Asn Gly Ser Leu Gln Cys Arg Ile Cys Ile 
                565                 570                 


<210>  188
<211>  1725
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PDI-B Vic-D193G DNA

<400>  188
atggcgaaaa acgttgcgat tttcggctta ttgttttctc ttcttgtgtt ggttccttct       60

cagatcttcg cggatcgaat ctgcactggg ataacatcgt caaactcacc acatgtcgtc      120

aaaactgcta ctcaagggga ggtcaatgtg accggtgtaa taccactgac aacaacaccc      180

accaaatctc attttgcaaa tctcaaagga acagaaacca gggggaaact atgcccaaaa      240

tgcctcaact gcacagatct ggatgtagcc ttgagcagac caaaatgcac agggaaaata      300

ccctctgcaa gggtttcaat actccatgaa gtcagacctg ttacatctgg gtgctttcct      360

ataatgcacg atagaacaaa aattagacag ctgcctaacc ttctccgagg atacgaacat      420

gtcaggttat caactcacaa cgttatcaat gcagaagatg caccaggagg accctacgag      480

attggaacct cagggtcttg ccctaacatt accaatggaa acggattctt cgcaacaatg      540

gcttgggccg tcccaaaaaa caaaacagca acaaatccat taacaataga agtaccatac      600

atttgtacag aaggagaaga ccaaattacc gtttgggggt tccactctgg caacgagacc      660

caaatggcaa agctctatgg ggactcaaag ccccagaagt tcacctcatc tgccaacgga      720

gtgaccacac attacgtttc acagattggt ggcttcccaa atcaaacaga agacggagga      780

ctaccacaaa gtggcagaat tgttgttgat tacatggtgc aaaaatctgg aaaaacagga      840

acaattacct atcaaagagg tattttattg cctcaaaagg tgtggtgcgc aagtggcagg      900

agcaaggtaa taaaaggatc cttgccttta attggagaag cagattgcct ccatgaaaaa      960

tacggtggat taaacaaaag caagccttac tacacagggg aacatgcaaa ggccatagga     1020

aattgcccaa tatgggtgaa aacacccttg aagctggcca atggaaccaa atatagacct     1080

cctggtggag gatgggaagg aatgattgca ggttggcacg gatacacatc ccatggggca     1140

catggagtag cggtggcagc agaccttaag agcactcaag aggccataaa caagataaca     1200

aaaaatctca actctttgag tgagctggaa gtaaagaatc ttcaaagact aagcggtgcc     1260

atggatgaac tccacaacga aatactagaa ctagatgaga aagtggatga tctcagagct     1320

gatacaataa gctcacaaat agaactcgca gtcctgcttt ccaatgaagg aataataaac     1380

agtgaagatg aacatctctt ggcgcttgaa agaaagctga agaaaatgct gggcccctct     1440

gctgtagaga tagggaatgg atgctttgaa accaaacaca agtgcaacca gacctgtctc     1500

gacagaatag ctgctggtac ctttgatgca ggagaatttt ctctccccac ctttgattca     1560

ctgaatatta ctgctgcatc tttaaatgac gatggattgg ataattacca gattttggcg     1620

atctattcaa ctgtcgccag ttcattggta ctggtagtct ccctgggggc aatcagtttc     1680

tggatgtgct ctaatgggtc tctacagtgt agaatatgta tttaa                     1725


<210>  189
<211>  574
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  PDI-B Vic-D193G AA

<400>  189

Met Ala Lys Asn Val Ala Ile Phe Gly Leu Leu Phe Ser Leu Leu Val 
1               5                   10                  15      


Leu Val Pro Ser Gln Ile Phe Ala Asp Arg Ile Cys Thr Gly Ile Thr 
            20                  25                  30          


Ser Ser Asn Ser Pro His Val Val Lys Thr Ala Thr Gln Gly Glu Val 
        35                  40                  45              


Asn Val Thr Gly Val Ile Pro Leu Thr Thr Thr Pro Thr Lys Ser His 
    50                  55                  60                  


Phe Ala Asn Leu Lys Gly Thr Glu Thr Arg Gly Lys Leu Cys Pro Lys 
65                  70                  75                  80  


Cys Leu Asn Cys Thr Asp Leu Asp Val Ala Leu Ser Arg Pro Lys Cys 
                85                  90                  95      


Thr Gly Lys Ile Pro Ser Ala Arg Val Ser Ile Leu His Glu Val Arg 
            100                 105                 110         


Pro Val Thr Ser Gly Cys Phe Pro Ile Met His Asp Arg Thr Lys Ile 
        115                 120                 125             


Arg Gln Leu Pro Asn Leu Leu Arg Gly Tyr Glu His Val Arg Leu Ser 
    130                 135                 140                 


Thr His Asn Val Ile Asn Ala Glu Asp Ala Pro Gly Gly Pro Tyr Glu 
145                 150                 155                 160 


Ile Gly Thr Ser Gly Ser Cys Pro Asn Ile Thr Asn Gly Asn Gly Phe 
                165                 170                 175     


Phe Ala Thr Met Ala Trp Ala Val Pro Lys Asn Lys Thr Ala Thr Asn 
            180                 185                 190         


Pro Leu Thr Ile Glu Val Pro Tyr Ile Cys Thr Glu Gly Glu Asp Gln 
        195                 200                 205             


Ile Thr Val Trp Gly Phe His Ser Gly Asn Glu Thr Gln Met Ala Lys 
    210                 215                 220                 


Leu Tyr Gly Asp Ser Lys Pro Gln Lys Phe Thr Ser Ser Ala Asn Gly 
225                 230                 235                 240 


Val Thr Thr His Tyr Val Ser Gln Ile Gly Gly Phe Pro Asn Gln Thr 
                245                 250                 255     


Glu Asp Gly Gly Leu Pro Gln Ser Gly Arg Ile Val Val Asp Tyr Met 
            260                 265                 270         


Val Gln Lys Ser Gly Lys Thr Gly Thr Ile Thr Tyr Gln Arg Gly Ile 
        275                 280                 285             


Leu Leu Pro Gln Lys Val Trp Cys Ala Ser Gly Arg Ser Lys Val Ile 
    290                 295                 300                 


Lys Gly Ser Leu Pro Leu Ile Gly Glu Ala Asp Cys Leu His Glu Lys 
305                 310                 315                 320 


Tyr Gly Gly Leu Asn Lys Ser Lys Pro Tyr Tyr Thr Gly Glu His Ala 
                325                 330                 335     


Lys Ala Ile Gly Asn Cys Pro Ile Trp Val Lys Thr Pro Leu Lys Leu 
            340                 345                 350         


Ala Asn Gly Thr Lys Tyr Arg Pro Pro Gly Gly Gly Trp Glu Gly Met 
        355                 360                 365             


Ile Ala Gly Trp His Gly Tyr Thr Ser His Gly Ala His Gly Val Ala 
    370                 375                 380                 


Val Ala Ala Asp Leu Lys Ser Thr Gln Glu Ala Ile Asn Lys Ile Thr 
385                 390                 395                 400 


Lys Asn Leu Asn Ser Leu Ser Glu Leu Glu Val Lys Asn Leu Gln Arg 
                405                 410                 415     


Leu Ser Gly Ala Met Asp Glu Leu His Asn Glu Ile Leu Glu Leu Asp 
            420                 425                 430         


Glu Lys Val Asp Asp Leu Arg Ala Asp Thr Ile Ser Ser Gln Ile Glu 
        435                 440                 445             


Leu Ala Val Leu Leu Ser Asn Glu Gly Ile Ile Asn Ser Glu Asp Glu 
    450                 455                 460                 


His Leu Leu Ala Leu Glu Arg Lys Leu Lys Lys Met Leu Gly Pro Ser 
465                 470                 475                 480 


Ala Val Glu Ile Gly Asn Gly Cys Phe Glu Thr Lys His Lys Cys Asn 
                485                 490                 495     


Gln Thr Cys Leu Asp Arg Ile Ala Ala Gly Thr Phe Asp Ala Gly Glu 
            500                 505                 510         


Phe Ser Leu Pro Thr Phe Asp Ser Leu Asn Ile Thr Ala Ala Ser Leu 
        515                 520                 525             


Asn Asp Asp Gly Leu Asp Asn Tyr Gln Ile Leu Ala Ile Tyr Ser Thr 
    530                 535                 540                 


Val Ala Ser Ser Leu Val Leu Val Val Ser Leu Gly Ala Ile Ser Phe 
545                 550                 555                 560 


Trp Met Cys Ser Asn Gly Ser Leu Gln Cys Arg Ile Cys Ile 
                565                 570                 


<210>  190
<211>  1725
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PDI-B Vic-L201A DNA

<400>  190
atggcgaaaa acgttgcgat tttcggctta ttgttttctc ttcttgtgtt ggttccttct       60

cagatcttcg cggatcgaat ctgcactggg ataacatcgt caaactcacc acatgtcgtc      120

aaaactgcta ctcaagggga ggtcaatgtg accggtgtaa taccactgac aacaacaccc      180

accaaatctc attttgcaaa tctcaaagga acagaaacca gggggaaact atgcccaaaa      240

tgcctcaact gcacagatct ggatgtagcc ttgagcagac caaaatgcac agggaaaata      300

ccctctgcaa gggtttcaat actccatgaa gtcagacctg ttacatctgg gtgctttcct      360

ataatgcacg atagaacaaa aattagacag ctgcctaacc ttctccgagg atacgaacat      420

gtcaggttat caactcacaa cgttatcaat gcagaagatg caccaggagg accctacgag      480

attggaacct cagggtcttg ccctaacatt accaatggaa acggattctt cgcaacaatg      540

gcttgggccg tcccaaaaaa caaaacagca acaaatccat taacaataga agtaccatac      600

atttgtacag aaggagaaga ccaaattacc gtttgggggt tccactctga caacgagacc      660

caaatggcaa aggcctatgg ggactcaaag ccccagaagt tcacctcatc tgccaacgga      720

gtgaccacac attacgtttc acagattggt ggcttcccaa atcaaacaga agacggagga      780

ctaccacaaa gtggcagaat tgttgttgat tacatggtgc aaaaatctgg aaaaacagga      840

acaattacct atcaaagagg tattttattg cctcaaaagg tgtggtgcgc aagtggcagg      900

agcaaggtaa taaaaggatc cttgccttta attggagaag cagattgcct ccatgaaaaa      960

tacggtggat taaacaaaag caagccttac tacacagggg aacatgcaaa ggccatagga     1020

aattgcccaa tatgggtgaa aacacccttg aagctggcca atggaaccaa atatagacct     1080

cctggtggag gatgggaagg aatgattgca ggttggcacg gatacacatc ccatggggca     1140

catggagtag cggtggcagc agaccttaag agcactcaag aggccataaa caagataaca     1200

aaaaatctca actctttgag tgagctggaa gtaaagaatc ttcaaagact aagcggtgcc     1260

atggatgaac tccacaacga aatactagaa ctagatgaga aagtggatga tctcagagct     1320

gatacaataa gctcacaaat agaactcgca gtcctgcttt ccaatgaagg aataataaac     1380

agtgaagatg aacatctctt ggcgcttgaa agaaagctga agaaaatgct gggcccctct     1440

gctgtagaga tagggaatgg atgctttgaa accaaacaca agtgcaacca gacctgtctc     1500

gacagaatag ctgctggtac ctttgatgca ggagaatttt ctctccccac ctttgattca     1560

ctgaatatta ctgctgcatc tttaaatgac gatggattgg ataattacca gattttggcg     1620

atctattcaa ctgtcgccag ttcattggta ctggtagtct ccctgggggc aatcagtttc     1680

tggatgtgct ctaatgggtc tctacagtgt agaatatgta tttaa                     1725


<210>  191
<211>  574
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  PDI-B Vic-L201A AA

<400>  191

Met Ala Lys Asn Val Ala Ile Phe Gly Leu Leu Phe Ser Leu Leu Val 
1               5                   10                  15      


Leu Val Pro Ser Gln Ile Phe Ala Asp Arg Ile Cys Thr Gly Ile Thr 
            20                  25                  30          


Ser Ser Asn Ser Pro His Val Val Lys Thr Ala Thr Gln Gly Glu Val 
        35                  40                  45              


Asn Val Thr Gly Val Ile Pro Leu Thr Thr Thr Pro Thr Lys Ser His 
    50                  55                  60                  


Phe Ala Asn Leu Lys Gly Thr Glu Thr Arg Gly Lys Leu Cys Pro Lys 
65                  70                  75                  80  


Cys Leu Asn Cys Thr Asp Leu Asp Val Ala Leu Ser Arg Pro Lys Cys 
                85                  90                  95      


Thr Gly Lys Ile Pro Ser Ala Arg Val Ser Ile Leu His Glu Val Arg 
            100                 105                 110         


Pro Val Thr Ser Gly Cys Phe Pro Ile Met His Asp Arg Thr Lys Ile 
        115                 120                 125             


Arg Gln Leu Pro Asn Leu Leu Arg Gly Tyr Glu His Val Arg Leu Ser 
    130                 135                 140                 


Thr His Asn Val Ile Asn Ala Glu Asp Ala Pro Gly Gly Pro Tyr Glu 
145                 150                 155                 160 


Ile Gly Thr Ser Gly Ser Cys Pro Asn Ile Thr Asn Gly Asn Gly Phe 
                165                 170                 175     


Phe Ala Thr Met Ala Trp Ala Val Pro Lys Asn Lys Thr Ala Thr Asn 
            180                 185                 190         


Pro Leu Thr Ile Glu Val Pro Tyr Ile Cys Thr Glu Gly Glu Asp Gln 
        195                 200                 205             


Ile Thr Val Trp Gly Phe His Ser Asp Asn Glu Thr Gln Met Ala Lys 
    210                 215                 220                 


Ala Tyr Gly Asp Ser Lys Pro Gln Lys Phe Thr Ser Ser Ala Asn Gly 
225                 230                 235                 240 


Val Thr Thr His Tyr Val Ser Gln Ile Gly Gly Phe Pro Asn Gln Thr 
                245                 250                 255     


Glu Asp Gly Gly Leu Pro Gln Ser Gly Arg Ile Val Val Asp Tyr Met 
            260                 265                 270         


Val Gln Lys Ser Gly Lys Thr Gly Thr Ile Thr Tyr Gln Arg Gly Ile 
        275                 280                 285             


Leu Leu Pro Gln Lys Val Trp Cys Ala Ser Gly Arg Ser Lys Val Ile 
    290                 295                 300                 


Lys Gly Ser Leu Pro Leu Ile Gly Glu Ala Asp Cys Leu His Glu Lys 
305                 310                 315                 320 


Tyr Gly Gly Leu Asn Lys Ser Lys Pro Tyr Tyr Thr Gly Glu His Ala 
                325                 330                 335     


Lys Ala Ile Gly Asn Cys Pro Ile Trp Val Lys Thr Pro Leu Lys Leu 
            340                 345                 350         


Ala Asn Gly Thr Lys Tyr Arg Pro Pro Gly Gly Gly Trp Glu Gly Met 
        355                 360                 365             


Ile Ala Gly Trp His Gly Tyr Thr Ser His Gly Ala His Gly Val Ala 
    370                 375                 380                 


Val Ala Ala Asp Leu Lys Ser Thr Gln Glu Ala Ile Asn Lys Ile Thr 
385                 390                 395                 400 


Lys Asn Leu Asn Ser Leu Ser Glu Leu Glu Val Lys Asn Leu Gln Arg 
                405                 410                 415     


Leu Ser Gly Ala Met Asp Glu Leu His Asn Glu Ile Leu Glu Leu Asp 
            420                 425                 430         


Glu Lys Val Asp Asp Leu Arg Ala Asp Thr Ile Ser Ser Gln Ile Glu 
        435                 440                 445             


Leu Ala Val Leu Leu Ser Asn Glu Gly Ile Ile Asn Ser Glu Asp Glu 
    450                 455                 460                 


His Leu Leu Ala Leu Glu Arg Lys Leu Lys Lys Met Leu Gly Pro Ser 
465                 470                 475                 480 


Ala Val Glu Ile Gly Asn Gly Cys Phe Glu Thr Lys His Lys Cys Asn 
                485                 490                 495     


Gln Thr Cys Leu Asp Arg Ile Ala Ala Gly Thr Phe Asp Ala Gly Glu 
            500                 505                 510         


Phe Ser Leu Pro Thr Phe Asp Ser Leu Asn Ile Thr Ala Ala Ser Leu 
        515                 520                 525             


Asn Asp Asp Gly Leu Asp Asn Tyr Gln Ile Leu Ala Ile Tyr Ser Thr 
    530                 535                 540                 


Val Ala Ser Ser Leu Val Leu Val Val Ser Leu Gly Ala Ile Ser Phe 
545                 550                 555                 560 


Trp Met Cys Ser Asn Gly Ser Leu Gln Cys Arg Ile Cys Ile 
                565                 570                 


<210>  192
<211>  1725
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PDI-B Vic-L201W DNA

<400>  192
atggcgaaaa acgttgcgat tttcggctta ttgttttctc ttcttgtgtt ggttccttct       60

cagatcttcg cggatcgaat ctgcactggg ataacatcgt caaactcacc acatgtcgtc      120

aaaactgcta ctcaagggga ggtcaatgtg accggtgtaa taccactgac aacaacaccc      180

accaaatctc attttgcaaa tctcaaagga acagaaacca gggggaaact atgcccaaaa      240

tgcctcaact gcacagatct ggatgtagcc ttgagcagac caaaatgcac agggaaaata      300

ccctctgcaa gggtttcaat actccatgaa gtcagacctg ttacatctgg gtgctttcct      360

ataatgcacg atagaacaaa aattagacag ctgcctaacc ttctccgagg atacgaacat      420

gtcaggttat caactcacaa cgttatcaat gcagaagatg caccaggagg accctacgag      480

attggaacct cagggtcttg ccctaacatt accaatggaa acggattctt cgcaacaatg      540

gcttgggccg tcccaaaaaa caaaacagca acaaatccat taacaataga agtaccatac      600

atttgtacag aaggagaaga ccaaattacc gtttgggggt tccactctga caacgagacc      660

caaatggcaa agtggtatgg ggactcaaag ccccagaagt tcacctcatc tgccaacgga      720

gtgaccacac attacgtttc acagattggt ggcttcccaa atcaaacaga agacggagga      780

ctaccacaaa gtggcagaat tgttgttgat tacatggtgc aaaaatctgg aaaaacagga      840

acaattacct atcaaagagg tattttattg cctcaaaagg tgtggtgcgc aagtggcagg      900

agcaaggtaa taaaaggatc cttgccttta attggagaag cagattgcct ccatgaaaaa      960

tacggtggat taaacaaaag caagccttac tacacagggg aacatgcaaa ggccatagga     1020

aattgcccaa tatgggtgaa aacacccttg aagctggcca atggaaccaa atatagacct     1080

cctggtggag gatgggaagg aatgattgca ggttggcacg gatacacatc ccatggggca     1140

catggagtag cggtggcagc agaccttaag agcactcaag aggccataaa caagataaca     1200

aaaaatctca actctttgag tgagctggaa gtaaagaatc ttcaaagact aagcggtgcc     1260

atggatgaac tccacaacga aatactagaa ctagatgaga aagtggatga tctcagagct     1320

gatacaataa gctcacaaat agaactcgca gtcctgcttt ccaatgaagg aataataaac     1380

agtgaagatg aacatctctt ggcgcttgaa agaaagctga agaaaatgct gggcccctct     1440

gctgtagaga tagggaatgg atgctttgaa accaaacaca agtgcaacca gacctgtctc     1500

gacagaatag ctgctggtac ctttgatgca ggagaatttt ctctccccac ctttgattca     1560

ctgaatatta ctgctgcatc tttaaatgac gatggattgg ataattacca gattttggcg     1620

atctattcaa ctgtcgccag ttcattggta ctggtagtct ccctgggggc aatcagtttc     1680

tggatgtgct ctaatgggtc tctacagtgt agaatatgta tttaa                     1725


<210>  193
<211>  574
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  PDI-B Vic-L201W AA

<400>  193

Met Ala Lys Asn Val Ala Ile Phe Gly Leu Leu Phe Ser Leu Leu Val 
1               5                   10                  15      


Leu Val Pro Ser Gln Ile Phe Ala Asp Arg Ile Cys Thr Gly Ile Thr 
            20                  25                  30          


Ser Ser Asn Ser Pro His Val Val Lys Thr Ala Thr Gln Gly Glu Val 
        35                  40                  45              


Asn Val Thr Gly Val Ile Pro Leu Thr Thr Thr Pro Thr Lys Ser His 
    50                  55                  60                  


Phe Ala Asn Leu Lys Gly Thr Glu Thr Arg Gly Lys Leu Cys Pro Lys 
65                  70                  75                  80  


Cys Leu Asn Cys Thr Asp Leu Asp Val Ala Leu Ser Arg Pro Lys Cys 
                85                  90                  95      


Thr Gly Lys Ile Pro Ser Ala Arg Val Ser Ile Leu His Glu Val Arg 
            100                 105                 110         


Pro Val Thr Ser Gly Cys Phe Pro Ile Met His Asp Arg Thr Lys Ile 
        115                 120                 125             


Arg Gln Leu Pro Asn Leu Leu Arg Gly Tyr Glu His Val Arg Leu Ser 
    130                 135                 140                 


Thr His Asn Val Ile Asn Ala Glu Asp Ala Pro Gly Gly Pro Tyr Glu 
145                 150                 155                 160 


Ile Gly Thr Ser Gly Ser Cys Pro Asn Ile Thr Asn Gly Asn Gly Phe 
                165                 170                 175     


Phe Ala Thr Met Ala Trp Ala Val Pro Lys Asn Lys Thr Ala Thr Asn 
            180                 185                 190         


Pro Leu Thr Ile Glu Val Pro Tyr Ile Cys Thr Glu Gly Glu Asp Gln 
        195                 200                 205             


Ile Thr Val Trp Gly Phe His Ser Asp Asn Glu Thr Gln Met Ala Lys 
    210                 215                 220                 


Trp Tyr Gly Asp Ser Lys Pro Gln Lys Phe Thr Ser Ser Ala Asn Gly 
225                 230                 235                 240 


Val Thr Thr His Tyr Val Ser Gln Ile Gly Gly Phe Pro Asn Gln Thr 
                245                 250                 255     


Glu Asp Gly Gly Leu Pro Gln Ser Gly Arg Ile Val Val Asp Tyr Met 
            260                 265                 270         


Val Gln Lys Ser Gly Lys Thr Gly Thr Ile Thr Tyr Gln Arg Gly Ile 
        275                 280                 285             


Leu Leu Pro Gln Lys Val Trp Cys Ala Ser Gly Arg Ser Lys Val Ile 
    290                 295                 300                 


Lys Gly Ser Leu Pro Leu Ile Gly Glu Ala Asp Cys Leu His Glu Lys 
305                 310                 315                 320 


Tyr Gly Gly Leu Asn Lys Ser Lys Pro Tyr Tyr Thr Gly Glu His Ala 
                325                 330                 335     


Lys Ala Ile Gly Asn Cys Pro Ile Trp Val Lys Thr Pro Leu Lys Leu 
            340                 345                 350         


Ala Asn Gly Thr Lys Tyr Arg Pro Pro Gly Gly Gly Trp Glu Gly Met 
        355                 360                 365             


Ile Ala Gly Trp His Gly Tyr Thr Ser His Gly Ala His Gly Val Ala 
    370                 375                 380                 


Val Ala Ala Asp Leu Lys Ser Thr Gln Glu Ala Ile Asn Lys Ile Thr 
385                 390                 395                 400 


Lys Asn Leu Asn Ser Leu Ser Glu Leu Glu Val Lys Asn Leu Gln Arg 
                405                 410                 415     


Leu Ser Gly Ala Met Asp Glu Leu His Asn Glu Ile Leu Glu Leu Asp 
            420                 425                 430         


Glu Lys Val Asp Asp Leu Arg Ala Asp Thr Ile Ser Ser Gln Ile Glu 
        435                 440                 445             


Leu Ala Val Leu Leu Ser Asn Glu Gly Ile Ile Asn Ser Glu Asp Glu 
    450                 455                 460                 


His Leu Leu Ala Leu Glu Arg Lys Leu Lys Lys Met Leu Gly Pro Ser 
465                 470                 475                 480 


Ala Val Glu Ile Gly Asn Gly Cys Phe Glu Thr Lys His Lys Cys Asn 
                485                 490                 495     


Gln Thr Cys Leu Asp Arg Ile Ala Ala Gly Thr Phe Asp Ala Gly Glu 
            500                 505                 510         


Phe Ser Leu Pro Thr Phe Asp Ser Leu Asn Ile Thr Ala Ala Ser Leu 
        515                 520                 525             


Asn Asp Asp Gly Leu Asp Asn Tyr Gln Ile Leu Ala Ile Tyr Ser Thr 
    530                 535                 540                 


Val Ala Ser Ser Leu Val Leu Val Val Ser Leu Gly Ala Ile Ser Phe 
545                 550                 555                 560 


Trp Met Cys Ser Asn Gly Ser Leu Gln Cys Arg Ile Cys Ile 
                565                 570                 


<210>  194
<211>  1722
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PDI-H1 Bris DNA

<400>  194
atggcgaaaa acgttgcgat tttcggctta ttgttttctc ttcttgtgtt ggttccttct       60

cagatcttcg cggacacatt atgtataggt tatcatgcga acaattcaac agacactgta      120

gacacagtac tagaaaagaa tgtaacagta acacactctg ttaaccttct ggaagacaag      180

cataacggaa aactatgcaa actaggcggg gtagccccat tgcatttggg taaatgtaac      240

attgctggct ggatcctggg aaatccagag tgtgaatcac tctccacagc aagatcatgg      300

tcctacattg tggaaacatc taattcagac aatggaacgt gttacccagg agatttcatc      360

aattatgagg agctaagaga gcaattgagc tcagtgtcat catttgaaag gtttgagata      420

ttccccaaga caagttcatg gcccaatcat gactcgaaca aaggtgtaac ggcagcatgt      480

cctcacgctg gagcaaaaag cttctacaaa aacttgatat ggctagttaa aaaaggaaat      540

tcatacccaa agcttaacca aacctacatt aatgataaag ggaaagaagt cctcgtgctg      600

tggggcattc accatccacc cactactgct gaccaacaaa gtctctatca gaatgcagat      660

gcatatgttt ttgtggggac atcaagatac agcaagaagt tcaagccgga aatagcaaca      720

agacccaaag tgagggatca agaagggaga atgaactatt actggacact agtagagccg      780

ggagacaaaa taacattcga agcaactgga aatctagtgg taccgagata tgcattcaca      840

atggaaagaa atgctggatc tggtattatc atttcagata caccagtcca cgattgcaat      900

acaacttgtc agacagccga gggtgctata aacaccagcc tcccatttca gaatgtgcat      960

ccggtgacaa ttggaaaatg tccaaagtat gtaaaaagca caaaattgag actggccaca     1020

ggattgagga atgttccgtc tattcaatct agaggcctat tcggggccat tgccggcttc     1080

attgaagggg ggtggacagg gatggtagat ggatggtacg gttatcacca tcaaaatgag     1140

caggggtcag gatatgcagc cgacctgaag agcacacaaa atgccattga caagattact     1200

aacaaagtaa attctgttat tgaaaagatg aatacacagg acacagcagt gggtaaagag     1260

ttcaaccacc tggaaaaaag aatagagaat ctaaataaaa aagttgatga tggtttcctg     1320

gacatttgga cttacaatgc cgaactgttg gttctaatgg aaaatgaaag aactttggac     1380

tatcacgatt caaatgtgaa gaacttgtat gaaaaagtaa gaaaccagtt aaaaaacaat     1440

gccaaggaaa ttggaaacgg ctgctttgaa ttttaccaca aatgcgataa cacgtgcatg     1500

gaaagtgtca aaaatgggac ttatgactac ccaaaatact cagaggaagc aaaattaaac     1560

agagaaaaaa tagatggggt aaagctggaa tcaacaagga tttaccagat tttggcgatc     1620

tattcaactg tcgccagttc attggtactg gtagtctccc tgggggcaat cagcttctgg     1680

atgtgctcta atgggtctct acagtgtaga atatgtattt aa                        1722


<210>  195
<211>  573
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  PDI-H1 Bris AA

<400>  195

Met Ala Lys Asn Val Ala Ile Phe Gly Leu Leu Phe Ser Leu Leu Val 
1               5                   10                  15      


Leu Val Pro Ser Gln Ile Phe Ala Asp Thr Leu Cys Ile Gly Tyr His 
            20                  25                  30          


Ala Asn Asn Ser Thr Asp Thr Val Asp Thr Val Leu Glu Lys Asn Val 
        35                  40                  45              


Thr Val Thr His Ser Val Asn Leu Leu Glu Asp Lys His Asn Gly Lys 
    50                  55                  60                  


Leu Cys Lys Leu Gly Gly Val Ala Pro Leu His Leu Gly Lys Cys Asn 
65                  70                  75                  80  


Ile Ala Gly Trp Ile Leu Gly Asn Pro Glu Cys Glu Ser Leu Ser Thr 
                85                  90                  95      


Ala Arg Ser Trp Ser Tyr Ile Val Glu Thr Ser Asn Ser Asp Asn Gly 
            100                 105                 110         


Thr Cys Tyr Pro Gly Asp Phe Ile Asn Tyr Glu Glu Leu Arg Glu Gln 
        115                 120                 125             


Leu Ser Ser Val Ser Ser Phe Glu Arg Phe Glu Ile Phe Pro Lys Thr 
    130                 135                 140                 


Ser Ser Trp Pro Asn His Asp Ser Asn Lys Gly Val Thr Ala Ala Cys 
145                 150                 155                 160 


Pro His Ala Gly Ala Lys Ser Phe Tyr Lys Asn Leu Ile Trp Leu Val 
                165                 170                 175     


Lys Lys Gly Asn Ser Tyr Pro Lys Leu Asn Gln Thr Tyr Ile Asn Asp 
            180                 185                 190         


Lys Gly Lys Glu Val Leu Val Leu Trp Gly Ile His His Pro Pro Thr 
        195                 200                 205             


Thr Ala Asp Gln Gln Ser Leu Tyr Gln Asn Ala Asp Ala Tyr Val Phe 
    210                 215                 220                 


Val Gly Thr Ser Arg Tyr Ser Lys Lys Phe Lys Pro Glu Ile Ala Thr 
225                 230                 235                 240 


Arg Pro Lys Val Arg Asp Gln Glu Gly Arg Met Asn Tyr Tyr Trp Thr 
                245                 250                 255     


Leu Val Glu Pro Gly Asp Lys Ile Thr Phe Glu Ala Thr Gly Asn Leu 
            260                 265                 270         


Val Val Pro Arg Tyr Ala Phe Thr Met Glu Arg Asn Ala Gly Ser Gly 
        275                 280                 285             


Ile Ile Ile Ser Asp Thr Pro Val His Asp Cys Asn Thr Thr Cys Gln 
    290                 295                 300                 


Thr Ala Glu Gly Ala Ile Asn Thr Ser Leu Pro Phe Gln Asn Val His 
305                 310                 315                 320 


Pro Val Thr Ile Gly Lys Cys Pro Lys Tyr Val Lys Ser Thr Lys Leu 
                325                 330                 335     


Arg Leu Ala Thr Gly Leu Arg Asn Val Pro Ser Ile Gln Ser Arg Gly 
            340                 345                 350         


Leu Phe Gly Ala Ile Ala Gly Phe Ile Glu Gly Gly Trp Thr Gly Met 
        355                 360                 365             


Val Asp Gly Trp Tyr Gly Tyr His His Gln Asn Glu Gln Gly Ser Gly 
    370                 375                 380                 


Tyr Ala Ala Asp Leu Lys Ser Thr Gln Asn Ala Ile Asp Lys Ile Thr 
385                 390                 395                 400 


Asn Lys Val Asn Ser Val Ile Glu Lys Met Asn Thr Gln Asp Thr Ala 
                405                 410                 415     


Val Gly Lys Glu Phe Asn His Leu Glu Lys Arg Ile Glu Asn Leu Asn 
            420                 425                 430         


Lys Lys Val Asp Asp Gly Phe Leu Asp Ile Trp Thr Tyr Asn Ala Glu 
        435                 440                 445             


Leu Leu Val Leu Met Glu Asn Glu Arg Thr Leu Asp Tyr His Asp Ser 
    450                 455                 460                 


Asn Val Lys Asn Leu Tyr Glu Lys Val Arg Asn Gln Leu Lys Asn Asn 
465                 470                 475                 480 


Ala Lys Glu Ile Gly Asn Gly Cys Phe Glu Phe Tyr His Lys Cys Asp 
                485                 490                 495     


Asn Thr Cys Met Glu Ser Val Lys Asn Gly Thr Tyr Asp Tyr Pro Lys 
            500                 505                 510         


Tyr Ser Glu Glu Ala Lys Leu Asn Arg Glu Lys Ile Asp Gly Val Lys 
        515                 520                 525             


Leu Glu Ser Thr Arg Ile Tyr Gln Ile Leu Ala Ile Tyr Ser Thr Val 
    530                 535                 540                 


Ala Ser Ser Leu Val Leu Val Val Ser Leu Gly Ala Ile Ser Phe Trp 
545                 550                 555                 560 


Met Cys Ser Asn Gly Ser Leu Gln Cys Arg Ile Cys Ile 
                565                 570             


<210>  196
<211>  1722
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PDI-H1 Bris-Y98F DNA

<400>  196
atggcgaaaa acgttgcgat tttcggctta ttgttttctc ttcttgtgtt ggttccttct       60

cagatcttcg cggacacatt atgtataggt tatcatgcga acaattcaac agacactgta      120

gacacagtac tagaaaagaa tgtaacagta acacactctg ttaaccttct ggaagacaag      180

cataacggaa aactatgcaa actaggcggg gtagccccat tgcatttggg taaatgtaac      240

attgctggct ggatcctggg aaatccagag tgtgaatcac tctccacagc aagatcatgg      300

tcctacattg tggaaacatc taattcagac aatggaacgt gtttcccagg agatttcatc      360

aattatgagg agctaagaga gcaattgagc tcagtgtcat catttgaaag gtttgagata      420

ttccccaaga caagttcatg gcccaatcat gactcgaaca aaggtgtaac ggcagcatgt      480

cctcacgctg gagcaaaaag cttctacaaa aacttgatat ggctagttaa aaaaggaaat      540

tcatacccaa agcttaacca aacctacatt aatgataaag ggaaagaagt cctcgtgctg      600

tggggcattc accatccacc cactactgct gaccaacaaa gtctctatca gaatgcagat      660

gcatatgttt ttgtggggac atcaagatac agcaagaagt tcaagccgga aatagcaaca      720

agacccaaag tgagggatca agaagggaga atgaactatt actggacact agtagagccg      780

ggagacaaaa taacattcga agcaactgga aatctagtgg taccgagata tgcattcaca      840

atggaaagaa atgctggatc tggtattatc atttcagata caccagtcca cgattgcaat      900

acaacttgtc agacagccga gggtgctata aacaccagcc tcccatttca gaatgtgcat      960

ccggtgacaa ttggaaaatg tccaaagtat gtaaaaagca caaaattgag actggccaca     1020

ggattgagga atgttccgtc tattcaatct agaggcctat tcggggccat tgccggcttc     1080

attgaagggg ggtggacagg gatggtagat ggatggtacg gttatcacca tcaaaatgag     1140

caggggtcag gatatgcagc cgacctgaag agcacacaaa atgccattga caagattact     1200

aacaaagtaa attctgttat tgaaaagatg aatacacagg acacagcagt gggtaaagag     1260

ttcaaccacc tggaaaaaag aatagagaat ctaaataaaa aagttgatga tggtttcctg     1320

gacatttgga cttacaatgc cgaactgttg gttctaatgg aaaatgaaag aactttggac     1380

tatcacgatt caaatgtgaa gaacttgtat gaaaaagtaa gaaaccagtt aaaaaacaat     1440

gccaaggaaa ttggaaacgg ctgctttgaa ttttaccaca aatgcgataa cacgtgcatg     1500

gaaagtgtca aaaatgggac ttatgactac ccaaaatact cagaggaagc aaaattaaac     1560

agagaaaaaa tagatggggt aaagctggaa tcaacaagga tttaccagat tttggcgatc     1620

tattcaactg tcgccagttc attggtactg gtagtctccc tgggggcaat cagcttctgg     1680

atgtgctcta atgggtctct acagtgtaga atatgtattt aa                        1722


<210>  197
<211>  573
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  PDI-H1 Bris-Y98F AA

<400>  197

Met Ala Lys Asn Val Ala Ile Phe Gly Leu Leu Phe Ser Leu Leu Val 
1               5                   10                  15      


Leu Val Pro Ser Gln Ile Phe Ala Asp Thr Leu Cys Ile Gly Tyr His 
            20                  25                  30          


Ala Asn Asn Ser Thr Asp Thr Val Asp Thr Val Leu Glu Lys Asn Val 
        35                  40                  45              


Thr Val Thr His Ser Val Asn Leu Leu Glu Asp Lys His Asn Gly Lys 
    50                  55                  60                  


Leu Cys Lys Leu Gly Gly Val Ala Pro Leu His Leu Gly Lys Cys Asn 
65                  70                  75                  80  


Ile Ala Gly Trp Ile Leu Gly Asn Pro Glu Cys Glu Ser Leu Ser Thr 
                85                  90                  95      


Ala Arg Ser Trp Ser Tyr Ile Val Glu Thr Ser Asn Ser Asp Asn Gly 
            100                 105                 110         


Thr Cys Phe Pro Gly Asp Phe Ile Asn Tyr Glu Glu Leu Arg Glu Gln 
        115                 120                 125             


Leu Ser Ser Val Ser Ser Phe Glu Arg Phe Glu Ile Phe Pro Lys Thr 
    130                 135                 140                 


Ser Ser Trp Pro Asn His Asp Ser Asn Lys Gly Val Thr Ala Ala Cys 
145                 150                 155                 160 


Pro His Ala Gly Ala Lys Ser Phe Tyr Lys Asn Leu Ile Trp Leu Val 
                165                 170                 175     


Lys Lys Gly Asn Ser Tyr Pro Lys Leu Asn Gln Thr Tyr Ile Asn Asp 
            180                 185                 190         


Lys Gly Lys Glu Val Leu Val Leu Trp Gly Ile His His Pro Pro Thr 
        195                 200                 205             


Thr Ala Asp Gln Gln Ser Leu Tyr Gln Asn Ala Asp Ala Tyr Val Phe 
    210                 215                 220                 


Val Gly Thr Ser Arg Tyr Ser Lys Lys Phe Lys Pro Glu Ile Ala Thr 
225                 230                 235                 240 


Arg Pro Lys Val Arg Asp Gln Glu Gly Arg Met Asn Tyr Tyr Trp Thr 
                245                 250                 255     


Leu Val Glu Pro Gly Asp Lys Ile Thr Phe Glu Ala Thr Gly Asn Leu 
            260                 265                 270         


Val Val Pro Arg Tyr Ala Phe Thr Met Glu Arg Asn Ala Gly Ser Gly 
        275                 280                 285             


Ile Ile Ile Ser Asp Thr Pro Val His Asp Cys Asn Thr Thr Cys Gln 
    290                 295                 300                 


Thr Ala Glu Gly Ala Ile Asn Thr Ser Leu Pro Phe Gln Asn Val His 
305                 310                 315                 320 


Pro Val Thr Ile Gly Lys Cys Pro Lys Tyr Val Lys Ser Thr Lys Leu 
                325                 330                 335     


Arg Leu Ala Thr Gly Leu Arg Asn Val Pro Ser Ile Gln Ser Arg Gly 
            340                 345                 350         


Leu Phe Gly Ala Ile Ala Gly Phe Ile Glu Gly Gly Trp Thr Gly Met 
        355                 360                 365             


Val Asp Gly Trp Tyr Gly Tyr His His Gln Asn Glu Gln Gly Ser Gly 
    370                 375                 380                 


Tyr Ala Ala Asp Leu Lys Ser Thr Gln Asn Ala Ile Asp Lys Ile Thr 
385                 390                 395                 400 


Asn Lys Val Asn Ser Val Ile Glu Lys Met Asn Thr Gln Asp Thr Ala 
                405                 410                 415     


Val Gly Lys Glu Phe Asn His Leu Glu Lys Arg Ile Glu Asn Leu Asn 
            420                 425                 430         


Lys Lys Val Asp Asp Gly Phe Leu Asp Ile Trp Thr Tyr Asn Ala Glu 
        435                 440                 445             


Leu Leu Val Leu Met Glu Asn Glu Arg Thr Leu Asp Tyr His Asp Ser 
    450                 455                 460                 


Asn Val Lys Asn Leu Tyr Glu Lys Val Arg Asn Gln Leu Lys Asn Asn 
465                 470                 475                 480 


Ala Lys Glu Ile Gly Asn Gly Cys Phe Glu Phe Tyr His Lys Cys Asp 
                485                 490                 495     


Asn Thr Cys Met Glu Ser Val Lys Asn Gly Thr Tyr Asp Tyr Pro Lys 
            500                 505                 510         


Tyr Ser Glu Glu Ala Lys Leu Asn Arg Glu Lys Ile Asp Gly Val Lys 
        515                 520                 525             


Leu Glu Ser Thr Arg Ile Tyr Gln Ile Leu Ala Ile Tyr Ser Thr Val 
    530                 535                 540                 


Ala Ser Ser Leu Val Leu Val Val Ser Leu Gly Ala Ile Ser Phe Trp 
545                 550                 555                 560 


Met Cys Ser Asn Gly Ser Leu Gln Cys Arg Ile Cys Ile 
                565                 570             


<210>  198
<211>  1731
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PDI-H5 Indo DNA

<400>  198
atggcgaaaa acgttgcgat tttcggctta ttgttttctc ttcttgtgtt ggttccttct       60

cagatcttcg ccgatcagat ttgcattggt taccatgcaa acaattcaac agagcaggtt      120

gacacaatca tggaaaagaa cgttactgtt acacatgccc aagacatact ggaaaagaca      180

cacaacggga agctctgcga tctagatgga gtgaagcctc taattttaag agattgtagt      240

gtagctggat ggctcctcgg gaacccaatg tgtgacgaat tcatcaatgt accggaatgg      300

tcttacatag tggagaaggc caatccaacc aatgacctct gttacccagg gagtttcaac      360

gactatgaag aactgaaaca cctattgagc agaataaacc attttgagaa aattcaaatc      420

atccccaaaa gttcttggtc cgatcatgaa gcctcatcag gagttagctc agcatgtcca      480

tacctgggaa gtccctcctt ttttagaaat gtggtatggc ttatcaaaaa gaacagtaca      540

tacccaacaa taaagaaaag ctacaataat accaaccaag aggatctttt ggtactgtgg      600

ggaattcacc atcctaatga tgcggcagag cagacaaggc tatatcaaaa cccaaccacc      660

tatatttcca ttgggacatc aacactaaac cagagattgg taccaaaaat agctactaga      720

tccaaagtaa acgggcaaag tggaaggatg gagttcttct ggacaatttt aaaacctaat      780

gatgcaatca acttcgagag taatggaaat ttcattgctc cagaatatgc atacaaaatt      840

gtcaagaaag gggactcagc aattatgaaa agtgaattgg aatatggtaa ctgcaacacc      900

aagtgtcaaa ctccaatggg ggcgataaac tctagtatgc cattccacaa catacaccct      960

ctcaccatcg gggaatgccc caaatatgtg aaatcaaaca gattagtcct tgcaacaggg     1020

ctcagaaata gccctcaaag agagagcaga agaaaaaaga gaggactatt tggagctata     1080

gcaggtttta tagagggagg atggcaggga atggtagatg gttggtatgg gtaccaccat     1140

agcaatgagc aggggagtgg gtacgctgca gacaaagaat ccactcaaaa ggcaatagat     1200

ggagtcacca ataaggtcaa ctcaatcatt gacaaaatga acactcagtt tgaggccgtt     1260

ggaagggaat ttaataactt agaaaggaga atagagaatt taaacaagaa gatggaagac     1320

gggtttctag atgtctggac ttataatgcc gaacttctgg ttctcatgga aaatgagaga     1380

actctagact ttcatgactc aaatgttaag aacctctacg acaaggtccg actacagctt     1440

agggataatg caaaggagct gggtaacggt tgtttcgagt tctatcacaa atgtgataat     1500

gaatgtatgg aaagtataag aaacggaacg tacaactatc cgcagtattc agaagaagca     1560

agattaaaaa gagaggaaat aagtggggta aaattggaat caataggaac ttaccaaata     1620

ctgtcaattt attcaacagt ggcgagttcc ctagcactgg caatcatgat ggctggtcta     1680

tctttatgga tgtgctccaa tggatcgtta caatgcagaa tttgcattta a              1731


<210>  199
<211>  576
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  PDI-H5 Indo AA

<400>  199

Met Ala Lys Asn Val Ala Ile Phe Gly Leu Leu Phe Ser Leu Leu Val 
1               5                   10                  15      


Leu Val Pro Ser Gln Ile Phe Ala Asp Gln Ile Cys Ile Gly Tyr His 
            20                  25                  30          


Ala Asn Asn Ser Thr Glu Gln Val Asp Thr Ile Met Glu Lys Asn Val 
        35                  40                  45              


Thr Val Thr His Ala Gln Asp Ile Leu Glu Lys Thr His Asn Gly Lys 
    50                  55                  60                  


Leu Cys Asp Leu Asp Gly Val Lys Pro Leu Ile Leu Arg Asp Cys Ser 
65                  70                  75                  80  


Val Ala Gly Trp Leu Leu Gly Asn Pro Met Cys Asp Glu Phe Ile Asn 
                85                  90                  95      


Val Pro Glu Trp Ser Tyr Ile Val Glu Lys Ala Asn Pro Thr Asn Asp 
            100                 105                 110         


Leu Cys Tyr Pro Gly Ser Phe Asn Asp Tyr Glu Glu Leu Lys His Leu 
        115                 120                 125             


Leu Ser Arg Ile Asn His Phe Glu Lys Ile Gln Ile Ile Pro Lys Ser 
    130                 135                 140                 


Ser Trp Ser Asp His Glu Ala Ser Ser Gly Val Ser Ser Ala Cys Pro 
145                 150                 155                 160 


Tyr Leu Gly Ser Pro Ser Phe Phe Arg Asn Val Val Trp Leu Ile Lys 
                165                 170                 175     


Lys Asn Ser Thr Tyr Pro Thr Ile Lys Lys Ser Tyr Asn Asn Thr Asn 
            180                 185                 190         


Gln Glu Asp Leu Leu Val Leu Trp Gly Ile His His Pro Asn Asp Ala 
        195                 200                 205             


Ala Glu Gln Thr Arg Leu Tyr Gln Asn Pro Thr Thr Tyr Ile Ser Ile 
    210                 215                 220                 


Gly Thr Ser Thr Leu Asn Gln Arg Leu Val Pro Lys Ile Ala Thr Arg 
225                 230                 235                 240 


Ser Lys Val Asn Gly Gln Ser Gly Arg Met Glu Phe Phe Trp Thr Ile 
                245                 250                 255     


Leu Lys Pro Asn Asp Ala Ile Asn Phe Glu Ser Asn Gly Asn Phe Ile 
            260                 265                 270         


Ala Pro Glu Tyr Ala Tyr Lys Ile Val Lys Lys Gly Asp Ser Ala Ile 
        275                 280                 285             


Met Lys Ser Glu Leu Glu Tyr Gly Asn Cys Asn Thr Lys Cys Gln Thr 
    290                 295                 300                 


Pro Met Gly Ala Ile Asn Ser Ser Met Pro Phe His Asn Ile His Pro 
305                 310                 315                 320 


Leu Thr Ile Gly Glu Cys Pro Lys Tyr Val Lys Ser Asn Arg Leu Val 
                325                 330                 335     


Leu Ala Thr Gly Leu Arg Asn Ser Pro Gln Arg Glu Ser Arg Arg Lys 
            340                 345                 350         


Lys Arg Gly Leu Phe Gly Ala Ile Ala Gly Phe Ile Glu Gly Gly Trp 
        355                 360                 365             


Gln Gly Met Val Asp Gly Trp Tyr Gly Tyr His His Ser Asn Glu Gln 
    370                 375                 380                 


Gly Ser Gly Tyr Ala Ala Asp Lys Glu Ser Thr Gln Lys Ala Ile Asp 
385                 390                 395                 400 


Gly Val Thr Asn Lys Val Asn Ser Ile Ile Asp Lys Met Asn Thr Gln 
                405                 410                 415     


Phe Glu Ala Val Gly Arg Glu Phe Asn Asn Leu Glu Arg Arg Ile Glu 
            420                 425                 430         


Asn Leu Asn Lys Lys Met Glu Asp Gly Phe Leu Asp Val Trp Thr Tyr 
        435                 440                 445             


Asn Ala Glu Leu Leu Val Leu Met Glu Asn Glu Arg Thr Leu Asp Phe 
    450                 455                 460                 


His Asp Ser Asn Val Lys Asn Leu Tyr Asp Lys Val Arg Leu Gln Leu 
465                 470                 475                 480 


Arg Asp Asn Ala Lys Glu Leu Gly Asn Gly Cys Phe Glu Phe Tyr His 
                485                 490                 495     


Lys Cys Asp Asn Glu Cys Met Glu Ser Ile Arg Asn Gly Thr Tyr Asn 
            500                 505                 510         


Tyr Pro Gln Tyr Ser Glu Glu Ala Arg Leu Lys Arg Glu Glu Ile Ser 
        515                 520                 525             


Gly Val Lys Leu Glu Ser Ile Gly Thr Tyr Gln Ile Leu Ser Ile Tyr 
    530                 535                 540                 


Ser Thr Val Ala Ser Ser Leu Ala Leu Ala Ile Met Met Ala Gly Leu 
545                 550                 555                 560 


Ser Leu Trp Met Cys Ser Asn Gly Ser Leu Gln Cys Arg Ile Cys Ile 
                565                 570                 575     


<210>  200
<211>  50
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  IF-H5ITMCT.s1-4r

<400>  200
actaaagaaa ataggccttt aaatgcaaat tctgcattgt aacgatccat                  50


<210>  201
<211>  1731
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PDI-H5 Indo-Y91F DNA

<400>  201
atggcgaaaa acgttgcgat tttcggctta ttgttttctc ttcttgtgtt ggttccttct       60

cagatcttcg ccgatcagat ttgcattggt taccatgcaa acaattcaac agagcaggtt      120

gacacaatca tggaaaagaa cgttactgtt acacatgccc aagacatact ggaaaagaca      180

cacaacggga agctctgcga tctagatgga gtgaagcctc taattttaag agattgtagt      240

gtagctggat ggctcctcgg gaacccaatg tgtgacgaat tcatcaatgt accggaatgg      300

tcttacatag tggagaaggc caatccaacc aatgacctct gtttcccagg gagtttcaac      360

gactatgaag aactgaaaca cctattgagc agaataaacc attttgagaa aattcaaatc      420

atccccaaaa gttcttggtc cgatcatgaa gcctcatcag gagttagctc agcatgtcca      480

tacctgggaa gtccctcctt ttttagaaat gtggtatggc ttatcaaaaa gaacagtaca      540

tacccaacaa taaagaaaag ctacaataat accaaccaag aggatctttt ggtactgtgg      600

ggaattcacc atcctaatga tgcggcagag cagacaaggc tatatcaaaa cccaaccacc      660

tatatttcca ttgggacatc aacactaaac cagagattgg taccaaaaat agctactaga      720

tccaaagtaa acgggcaaag tggaaggatg gagttcttct ggacaatttt aaaacctaat      780

gatgcaatca acttcgagag taatggaaat ttcattgctc cagaatatgc atacaaaatt      840

gtcaagaaag gggactcagc aattatgaaa agtgaattgg aatatggtaa ctgcaacacc      900

aagtgtcaaa ctccaatggg ggcgataaac tctagtatgc cattccacaa catacaccct      960

ctcaccatcg gggaatgccc caaatatgtg aaatcaaaca gattagtcct tgcaacaggg     1020

ctcagaaata gccctcaaag agagagcaga agaaaaaaga gaggactatt tggagctata     1080

gcaggtttta tagagggagg atggcaggga atggtagatg gttggtatgg gtaccaccat     1140

agcaatgagc aggggagtgg gtacgctgca gacaaagaat ccactcaaaa ggcaatagat     1200

ggagtcacca ataaggtcaa ctcaatcatt gacaaaatga acactcagtt tgaggccgtt     1260

ggaagggaat ttaataactt agaaaggaga atagagaatt taaacaagaa gatggaagac     1320

gggtttctag atgtctggac ttataatgcc gaacttctgg ttctcatgga aaatgagaga     1380

actctagact ttcatgactc aaatgttaag aacctctacg acaaggtccg actacagctt     1440

agggataatg caaaggagct gggtaacggt tgtttcgagt tctatcacaa atgtgataat     1500

gaatgtatgg aaagtataag aaacggaacg tacaactatc cgcagtattc agaagaagca     1560

agattaaaaa gagaggaaat aagtggggta aaattggaat caataggaac ttaccaaata     1620

ctgtcaattt attcaacagt ggcgagttcc ctagcactgg caatcatgat ggctggtcta     1680

tctttatgga tgtgctccaa tggatcgtta caatgcagaa tttgcattta a              1731


<210>  202
<211>  576
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  PDI-H5 Indo-Y91F AA

<400>  202

Met Ala Lys Asn Val Ala Ile Phe Gly Leu Leu Phe Ser Leu Leu Val 
1               5                   10                  15      


Leu Val Pro Ser Gln Ile Phe Ala Asp Gln Ile Cys Ile Gly Tyr His 
            20                  25                  30          


Ala Asn Asn Ser Thr Glu Gln Val Asp Thr Ile Met Glu Lys Asn Val 
        35                  40                  45              


Thr Val Thr His Ala Gln Asp Ile Leu Glu Lys Thr His Asn Gly Lys 
    50                  55                  60                  


Leu Cys Asp Leu Asp Gly Val Lys Pro Leu Ile Leu Arg Asp Cys Ser 
65                  70                  75                  80  


Val Ala Gly Trp Leu Leu Gly Asn Pro Met Cys Asp Glu Phe Ile Asn 
                85                  90                  95      


Val Pro Glu Trp Ser Tyr Ile Val Glu Lys Ala Asn Pro Thr Asn Asp 
            100                 105                 110         


Leu Cys Phe Pro Gly Ser Phe Asn Asp Tyr Glu Glu Leu Lys His Leu 
        115                 120                 125             


Leu Ser Arg Ile Asn His Phe Glu Lys Ile Gln Ile Ile Pro Lys Ser 
    130                 135                 140                 


Ser Trp Ser Asp His Glu Ala Ser Ser Gly Val Ser Ser Ala Cys Pro 
145                 150                 155                 160 


Tyr Leu Gly Ser Pro Ser Phe Phe Arg Asn Val Val Trp Leu Ile Lys 
                165                 170                 175     


Lys Asn Ser Thr Tyr Pro Thr Ile Lys Lys Ser Tyr Asn Asn Thr Asn 
            180                 185                 190         


Gln Glu Asp Leu Leu Val Leu Trp Gly Ile His His Pro Asn Asp Ala 
        195                 200                 205             


Ala Glu Gln Thr Arg Leu Tyr Gln Asn Pro Thr Thr Tyr Ile Ser Ile 
    210                 215                 220                 


Gly Thr Ser Thr Leu Asn Gln Arg Leu Val Pro Lys Ile Ala Thr Arg 
225                 230                 235                 240 


Ser Lys Val Asn Gly Gln Ser Gly Arg Met Glu Phe Phe Trp Thr Ile 
                245                 250                 255     


Leu Lys Pro Asn Asp Ala Ile Asn Phe Glu Ser Asn Gly Asn Phe Ile 
            260                 265                 270         


Ala Pro Glu Tyr Ala Tyr Lys Ile Val Lys Lys Gly Asp Ser Ala Ile 
        275                 280                 285             


Met Lys Ser Glu Leu Glu Tyr Gly Asn Cys Asn Thr Lys Cys Gln Thr 
    290                 295                 300                 


Pro Met Gly Ala Ile Asn Ser Ser Met Pro Phe His Asn Ile His Pro 
305                 310                 315                 320 


Leu Thr Ile Gly Glu Cys Pro Lys Tyr Val Lys Ser Asn Arg Leu Val 
                325                 330                 335     


Leu Ala Thr Gly Leu Arg Asn Ser Pro Gln Arg Glu Ser Arg Arg Lys 
            340                 345                 350         


Lys Arg Gly Leu Phe Gly Ala Ile Ala Gly Phe Ile Glu Gly Gly Trp 
        355                 360                 365             


Gln Gly Met Val Asp Gly Trp Tyr Gly Tyr His His Ser Asn Glu Gln 
    370                 375                 380                 


Gly Ser Gly Tyr Ala Ala Asp Lys Glu Ser Thr Gln Lys Ala Ile Asp 
385                 390                 395                 400 


Gly Val Thr Asn Lys Val Asn Ser Ile Ile Asp Lys Met Asn Thr Gln 
                405                 410                 415     


Phe Glu Ala Val Gly Arg Glu Phe Asn Asn Leu Glu Arg Arg Ile Glu 
            420                 425                 430         


Asn Leu Asn Lys Lys Met Glu Asp Gly Phe Leu Asp Val Trp Thr Tyr 
        435                 440                 445             


Asn Ala Glu Leu Leu Val Leu Met Glu Asn Glu Arg Thr Leu Asp Phe 
    450                 455                 460                 


His Asp Ser Asn Val Lys Asn Leu Tyr Asp Lys Val Arg Leu Gln Leu 
465                 470                 475                 480 


Arg Asp Asn Ala Lys Glu Leu Gly Asn Gly Cys Phe Glu Phe Tyr His 
                485                 490                 495     


Lys Cys Asp Asn Glu Cys Met Glu Ser Ile Arg Asn Gly Thr Tyr Asn 
            500                 505                 510         


Tyr Pro Gln Tyr Ser Glu Glu Ala Arg Leu Lys Arg Glu Glu Ile Ser 
        515                 520                 525             


Gly Val Lys Leu Glu Ser Ile Gly Thr Tyr Gln Ile Leu Ser Ile Tyr 
    530                 535                 540                 


Ser Thr Val Ala Ser Ser Leu Ala Leu Ala Ile Met Met Ala Gly Leu 
545                 550                 555                 560 


Ser Leu Trp Met Cys Ser Asn Gly Ser Leu Gln Cys Arg Ile Cys Ile 
                565                 570                 575     


<210>  203
<211>  549
<212>  PRT
<213>  Influenza Virus

<400>  203

Asp Thr Leu Cys Ile Gly Tyr His Ala Asn Asn Ser Thr Asp Thr Val 
1               5                   10                  15      


Asp Thr Val Leu Glu Lys Asn Val Thr Val Thr His Ser Val Asn Leu 
            20                  25                  30          


Leu Glu Asp Lys His Asn Gly Lys Leu Cys Lys Leu Arg Gly Val Ala 
        35                  40                  45              


Pro Leu His Leu Gly Lys Cys Asn Ile Ala Gly Trp Ile Leu Gly Asn 
    50                  55                  60                  


Pro Glu Cys Glu Ser Leu Ser Thr Ala Ser Ser Trp Ser Tyr Ile Val 
65                  70                  75                  80  


Glu Thr Pro Ser Ser Asp Asn Gly Thr Cys Tyr Pro Gly Asp Phe Ile 
                85                  90                  95      


Asp Tyr Glu Glu Leu Arg Glu Gln Leu Ser Ser Val Ser Ser Phe Glu 
            100                 105                 110         


Arg Phe Glu Ile Phe Pro Lys Thr Ser Ser Trp Pro Asn His Asp Ser 
        115                 120                 125             


Asn Lys Gly Val Thr Ala Ala Cys Pro His Ala Gly Ala Lys Ser Phe 
    130                 135                 140                 


Tyr Lys Asn Leu Ile Trp Leu Val Lys Lys Gly Asn Ser Tyr Pro Lys 
145                 150                 155                 160 


Leu Ser Lys Ser Tyr Ile Asn Asp Lys Gly Lys Glu Val Leu Val Leu 
                165                 170                 175     


Trp Gly Ile His His Pro Ser Thr Ser Ala Asp Gln Gln Ser Leu Tyr 
            180                 185                 190         


Gln Asn Ala Asp Ala Tyr Val Phe Val Gly Ser Ser Arg Tyr Ser Lys 
        195                 200                 205             


Lys Phe Lys Pro Glu Ile Ala Ile Arg Pro Lys Val Arg Asp Gln Glu 
    210                 215                 220                 


Gly Arg Met Asn Tyr Tyr Trp Thr Leu Val Glu Pro Gly Asp Lys Ile 
225                 230                 235                 240 


Thr Phe Glu Ala Thr Gly Asn Leu Val Val Pro Arg Tyr Ala Phe Ala 
                245                 250                 255     


Met Glu Arg Asn Ala Gly Ser Gly Ile Ile Ile Ser Asp Thr Pro Val 
            260                 265                 270         


His Asp Cys Asn Thr Thr Cys Gln Thr Pro Lys Gly Ala Ile Asn Thr 
        275                 280                 285             


Ser Leu Pro Phe Gln Asn Ile His Pro Ile Thr Ile Gly Lys Cys Pro 
    290                 295                 300                 


Lys Tyr Val Lys Ser Thr Lys Leu Arg Leu Ala Thr Gly Leu Arg Asn 
305                 310                 315                 320 


Ile Pro Ser Ile Gln Ser Arg Gly Leu Phe Gly Ala Ile Ala Gly Phe 
                325                 330                 335     


Ile Glu Gly Gly Trp Thr Gly Met Val Asp Gly Trp Tyr Gly Tyr His 
            340                 345                 350         


His Gln Asn Glu Gln Gly Ser Gly Tyr Ala Ala Asp Leu Lys Ser Thr 
        355                 360                 365             


Gln Asn Ala Ile Asp Glu Ile Thr Asn Lys Val Asn Ser Val Ile Glu 
    370                 375                 380                 


Lys Met Asn Thr Gln Phe Thr Ala Val Gly Lys Glu Phe Asn His Leu 
385                 390                 395                 400 


Glu Lys Arg Ile Glu Asn Leu Asn Lys Lys Val Asp Asp Gly Phe Leu 
                405                 410                 415     


Asp Ile Trp Thr Tyr Asn Ala Glu Leu Leu Val Leu Leu Glu Asn Glu 
            420                 425                 430         


Arg Thr Leu Asp Tyr His Asp Ser Asn Val Lys Asn Leu Tyr Glu Lys 
        435                 440                 445             


Val Arg Ser Gln Leu Lys Asn Asn Ala Lys Glu Ile Gly Asn Gly Cys 
    450                 455                 460                 


Phe Glu Phe Tyr His Lys Cys Asp Asn Thr Cys Met Glu Ser Val Lys 
465                 470                 475                 480 


Asn Gly Thr Tyr Asp Tyr Pro Lys Tyr Ser Glu Glu Ala Lys Leu Asn 
                485                 490                 495     


Arg Glu Glu Ile Asp Gly Val Lys Leu Glu Ser Thr Arg Ile Tyr Gln 
            500                 505                 510         


Ile Leu Ala Ile Tyr Ser Thr Val Ala Ser Ser Leu Val Leu Val Val 
        515                 520                 525             


Ser Leu Gly Ala Ile Ser Phe Trp Met Cys Ser Asn Gly Ser Leu Gln 
    530                 535                 540                 


Cys Arg Ile Cys Ile 
545                 


<210>  204
<211>  550
<212>  PRT
<213>  Influenza Virus

<400>  204

Gln Lys Ile Pro Gly Asn Asp Asn Ser Thr Ala Thr Leu Cys Leu Gly 
1               5                   10                  15      


His His Ala Val Pro Asn Gly Thr Ile Val Lys Thr Ile Thr Asn Asp 
            20                  25                  30          


Arg Ile Glu Val Thr Asn Ala Thr Glu Leu Val Gln Asn Ser Ser Ile 
        35                  40                  45              


Gly Glu Ile Cys Asp Ser Pro His Gln Ile Leu Asp Gly Glu Asn Cys 
    50                  55                  60                  


Thr Leu Ile Asp Ala Leu Leu Gly Asp Pro Gln Cys Asp Gly Phe Gln 
65                  70                  75                  80  


Asn Lys Lys Trp Asp Leu Phe Val Glu Arg Asn Lys Ala Tyr Ser Asn 
                85                  90                  95      


Cys Tyr Pro Tyr Asp Val Pro Asp Tyr Ala Ser Leu Arg Ser Leu Val 
            100                 105                 110         


Ala Ser Ser Gly Thr Leu Glu Phe Asn Asn Glu Ser Phe Asn Trp Ala 
        115                 120                 125             


Gly Val Thr Gln Asn Gly Thr Ser Ser Ser Cys Ile Arg Gly Ser Lys 
    130                 135                 140                 


Ser Ser Phe Phe Ser Arg Leu Asn Trp Leu Thr His Leu Asn Ser Lys 
145                 150                 155                 160 


Tyr Pro Ala Leu Asn Val Thr Met Pro Asn Asn Glu Gln Phe Asp Lys 
                165                 170                 175     


Leu Tyr Ile Trp Gly Val His His Pro Gly Thr Asp Lys Asp Gln Ile 
            180                 185                 190         


Ser Leu Tyr Ala Gln Ser Ser Gly Arg Ile Thr Val Ser Thr Lys Arg 
        195                 200                 205             


Ser Gln Gln Ala Val Ile Pro Asn Ile Gly Ser Arg Pro Arg Ile Arg 
    210                 215                 220                 


Asp Ile Pro Ser Arg Ile Ser Ile Tyr Trp Thr Ile Val Lys Pro Gly 
225                 230                 235                 240 


Asp Ile Leu Leu Ile Asn Ser Thr Gly Asn Leu Ile Ala Pro Arg Gly 
                245                 250                 255     


Tyr Phe Lys Ile Arg Ser Gly Lys Ser Ser Ile Met Arg Ser Asp Ala 
            260                 265                 270         


Pro Ile Gly Lys Cys Lys Ser Glu Cys Ile Thr Pro Asn Gly Ser Ile 
        275                 280                 285             


Pro Asn Asp Lys Pro Phe Gln Asn Val Asn Arg Ile Thr Tyr Gly Ala 
    290                 295                 300                 


Cys Pro Arg Tyr Val Lys Gln Ser Thr Leu Lys Leu Ala Thr Gly Met 
305                 310                 315                 320 


Arg Asn Val Pro Glu Arg Gln Thr Arg Gly Ile Phe Gly Ala Ile Ala 
                325                 330                 335     


Gly Phe Ile Glu Asn Gly Trp Glu Gly Met Val Asp Gly Trp Tyr Gly 
            340                 345                 350         


Phe Arg His Gln Asn Ser Glu Gly Arg Gly Gln Ala Ala Asp Leu Lys 
        355                 360                 365             


Ser Thr Gln Ala Ala Ile Asp Gln Ile Asn Gly Lys Leu Asn Arg Leu 
    370                 375                 380                 


Ile Gly Lys Thr Asn Glu Lys Phe His Gln Ile Glu Lys Glu Phe Ser 
385                 390                 395                 400 


Glu Val Glu Gly Arg Ile Gln Asp Leu Glu Lys Tyr Val Glu Asp Thr 
                405                 410                 415     


Lys Ile Asp Leu Trp Ser Tyr Asn Ala Glu Leu Leu Val Ala Leu Glu 
            420                 425                 430         


Asn Gln His Thr Ile Asp Leu Thr Asp Ser Glu Met Asn Lys Leu Phe 
        435                 440                 445             


Glu Lys Thr Lys Lys Gln Leu Arg Glu Asn Ala Glu Asp Met Gly Asn 
    450                 455                 460                 


Gly Cys Phe Lys Ile Tyr His Lys Cys Asp Asn Ala Cys Met Gly Ser 
465                 470                 475                 480 


Ile Arg Asn Gly Thr Tyr Asp His Asn Val Tyr Arg Asp Glu Ala Leu 
                485                 490                 495     


Asn Asn Arg Phe Gln Ile Lys Gly Val Glu Leu Lys Ser Gly Tyr Lys 
            500                 505                 510         


Asp Trp Ile Leu Trp Ile Ser Phe Ala Ile Ser Cys Phe Leu Leu Cys 
        515                 520                 525             


Val Ala Leu Leu Gly Phe Ile Met Trp Ala Cys Gln Lys Gly Asn Ile 
    530                 535                 540                 


Arg Cys Asn Ile Cys Ile 
545                 550 


<210>  205
<211>  552
<212>  PRT
<213>  Influenza Virus

<400>  205

Asp Gln Ile Cys Ile Gly Tyr His Ala Asn Asn Ser Thr Glu Gln Val 
1               5                   10                  15      


Asp Thr Ile Met Glu Lys Asn Val Thr Val Thr His Ala Gln Asp Ile 
            20                  25                  30          


Leu Glu Lys Thr His Asn Gly Lys Leu Cys Asp Leu Asp Gly Val Lys 
        35                  40                  45              


Pro Leu Ile Leu Arg Asp Cys Ser Val Ala Gly Trp Leu Leu Gly Asn 
    50                  55                  60                  


Pro Met Cys Asp Glu Phe Ile Asn Val Pro Glu Trp Ser Tyr Ile Val 
65                  70                  75                  80  


Glu Lys Ala Asn Pro Thr Asn Asp Leu Cys Tyr Pro Gly Ser Phe Asn 
                85                  90                  95      


Asp Tyr Glu Glu Leu Lys His Leu Leu Ser Arg Ile Asn His Phe Glu 
            100                 105                 110         


Lys Ile Gln Ile Ile Pro Lys Ser Ser Trp Ser Asp His Glu Ala Ser 
        115                 120                 125             


Ser Gly Val Ser Ser Ala Cys Pro Tyr Leu Gly Ser Pro Ser Phe Phe 
    130                 135                 140                 


Arg Asn Val Val Trp Leu Ile Lys Lys Asn Ser Thr Tyr Pro Thr Ile 
145                 150                 155                 160 


Lys Lys Ser Tyr Asn Asn Thr Asn Gln Glu Asp Leu Leu Val Leu Trp 
                165                 170                 175     


Gly Ile His His Pro Asn Asp Ala Ala Glu Gln Thr Arg Leu Tyr Gln 
            180                 185                 190         


Asn Pro Thr Thr Tyr Ile Ser Ile Gly Thr Ser Thr Leu Asn Gln Arg 
        195                 200                 205             


Leu Val Pro Lys Ile Ala Thr Arg Ser Lys Val Asn Gly Gln Ser Gly 
    210                 215                 220                 


Arg Met Glu Phe Phe Trp Thr Ile Leu Lys Pro Asn Asp Ala Ile Asn 
225                 230                 235                 240 


Phe Glu Ser Asn Gly Asn Phe Ile Ala Pro Glu Tyr Ala Tyr Lys Ile 
                245                 250                 255     


Val Lys Lys Gly Asp Ser Ala Ile Met Lys Ser Glu Leu Glu Tyr Gly 
            260                 265                 270         


Asn Cys Asn Thr Lys Cys Gln Thr Pro Met Gly Ala Ile Asn Ser Ser 
        275                 280                 285             


Met Pro Phe His Asn Ile His Pro Leu Thr Ile Gly Glu Cys Pro Lys 
    290                 295                 300                 


Tyr Val Lys Ser Asn Arg Leu Val Leu Ala Thr Gly Leu Arg Asn Ser 
305                 310                 315                 320 


Pro Gln Arg Glu Ser Arg Arg Lys Lys Arg Gly Leu Phe Gly Ala Ile 
                325                 330                 335     


Ala Gly Phe Ile Glu Gly Gly Trp Gln Gly Met Val Asp Gly Trp Tyr 
            340                 345                 350         


Gly Tyr His His Ser Asn Glu Gln Gly Ser Gly Tyr Ala Ala Asp Lys 
        355                 360                 365             


Glu Ser Thr Gln Lys Ala Ile Asp Gly Val Thr Asn Lys Val Asn Ser 
    370                 375                 380                 


Ile Ile Asp Lys Met Asn Thr Gln Phe Glu Ala Val Gly Arg Glu Phe 
385                 390                 395                 400 


Asn Asn Leu Glu Arg Arg Ile Glu Asn Leu Asn Lys Lys Met Glu Asp 
                405                 410                 415     


Gly Phe Leu Asp Val Trp Thr Tyr Asn Ala Glu Leu Leu Val Leu Met 
            420                 425                 430         


Glu Asn Glu Arg Thr Leu Asp Phe His Asp Ser Asn Val Lys Asn Leu 
        435                 440                 445             


Tyr Asp Lys Val Arg Leu Gln Leu Arg Asp Asn Ala Lys Glu Leu Gly 
    450                 455                 460                 


Asn Gly Cys Phe Glu Phe Tyr His Lys Cys Asp Asn Glu Cys Met Glu 
465                 470                 475                 480 


Ser Ile Arg Asn Gly Thr Tyr Asn Tyr Pro Gln Tyr Ser Glu Glu Ala 
                485                 490                 495     


Arg Leu Lys Arg Glu Glu Ile Ser Gly Val Lys Leu Glu Ser Ile Gly 
            500                 505                 510         


Thr Tyr Gln Ile Leu Ser Ile Tyr Ser Thr Val Ala Ser Ser Leu Ala 
        515                 520                 525             


Leu Ala Ile Met Met Ala Gly Leu Ser Leu Trp Met Cys Ser Asn Gly 
    530                 535                 540                 


Ser Leu Gln Cys Arg Ile Cys Ile 
545                 550         


<210>  206
<211>  542
<212>  PRT
<213>  Influenza Virus

<400>  206

Asp Lys Ile Cys Leu Gly His His Ala Val Ser Asn Gly Thr Lys Val 
1               5                   10                  15      


Asn Thr Leu Thr Glu Arg Gly Val Glu Val Val Asn Ala Thr Glu Thr 
            20                  25                  30          


Val Glu Arg Thr Asn Ile Pro Arg Ile Cys Ser Lys Gly Lys Arg Thr 
        35                  40                  45              


Val Asp Leu Gly Gln Cys Gly Leu Leu Gly Thr Ile Thr Gly Pro Pro 
    50                  55                  60                  


Gln Cys Asp Gln Phe Leu Glu Phe Ser Ala Asp Leu Ile Ile Glu Arg 
65                  70                  75                  80  


Arg Glu Gly Ser Asp Val Cys Tyr Pro Gly Lys Phe Val Asn Glu Glu 
                85                  90                  95      


Ala Leu Arg Gln Ile Leu Arg Glu Ser Gly Gly Ile Asp Lys Glu Ala 
            100                 105                 110         


Met Gly Phe Thr Tyr Ser Gly Ile Arg Thr Asn Gly Ala Thr Ser Ala 
        115                 120                 125             


Cys Arg Arg Ser Gly Ser Ser Phe Tyr Ala Glu Met Lys Trp Leu Leu 
    130                 135                 140                 


Ser Asn Thr Asp Asn Ala Ala Phe Pro Gln Met Thr Lys Ser Tyr Lys 
145                 150                 155                 160 


Asn Thr Arg Lys Ser Pro Ala Leu Ile Val Trp Gly Ile His His Ser 
                165                 170                 175     


Val Ser Thr Ala Glu Gln Thr Lys Leu Tyr Gly Ser Gly Asn Lys Leu 
            180                 185                 190         


Val Thr Val Gly Ser Ser Asn Tyr Gln Gln Ser Phe Val Pro Ser Pro 
        195                 200                 205             


Gly Ala Arg Pro Gln Val Asn Gly Leu Ser Gly Arg Ile Asp Phe His 
    210                 215                 220                 


Trp Leu Met Leu Asn Pro Asn Asp Thr Val Thr Phe Ser Phe Asn Gly 
225                 230                 235                 240 


Ala Phe Ile Ala Pro Asp Arg Ala Ser Phe Leu Arg Gly Lys Ser Met 
                245                 250                 255     


Gly Ile Gln Ser Gly Val Gln Val Asp Ala Asn Cys Glu Gly Asp Cys 
            260                 265                 270         


Tyr His Ser Gly Gly Thr Ile Ile Ser Asn Leu Pro Phe Gln Asn Ile 
        275                 280                 285             


Asp Ser Arg Ala Val Gly Lys Cys Pro Arg Tyr Val Lys Gln Arg Ser 
    290                 295                 300                 


Leu Leu Leu Ala Thr Gly Met Lys Asn Val Pro Glu Ile Pro Lys Gly 
305                 310                 315                 320 


Arg Gly Leu Phe Gly Ala Ile Ala Gly Phe Ile Glu Asn Gly Trp Glu 
                325                 330                 335     


Gly Leu Ile Asp Gly Trp Tyr Gly Phe Arg His Gln Asn Ala Gln Gly 
            340                 345                 350         


Glu Gly Thr Ala Ala Asp Tyr Lys Ser Thr Gln Ser Ala Ile Asp Gln 
        355                 360                 365             


Ile Thr Gly Lys Leu Asn Arg Leu Ile Glu Lys Thr Asn Gln Gln Phe 
    370                 375                 380                 


Glu Leu Ile Asp Asn Glu Phe Asn Glu Val Glu Lys Gln Ile Gly Asn 
385                 390                 395                 400 


Val Ile Asn Trp Thr Arg Asp Ser Ile Thr Glu Val Trp Ser Tyr Asn 
                405                 410                 415     


Ala Glu Leu Leu Val Ala Met Glu Asn Gln His Thr Ile Asp Leu Ala 
            420                 425                 430         


Asp Ser Glu Met Asp Lys Leu Tyr Glu Arg Val Lys Arg Gln Leu Arg 
        435                 440                 445             


Glu Asn Ala Glu Glu Asp Gly Thr Gly Cys Phe Glu Ile Phe His Lys 
    450                 455                 460                 


Cys Asp Asp Asp Cys Met Ala Ser Ile Arg Asn Asn Thr Tyr Asp His 
465                 470                 475                 480 


Ser Lys Tyr Arg Glu Glu Ala Met Gln Asn Arg Ile Gln Ile Asp Pro 
                485                 490                 495     


Val Lys Leu Ser Ser Gly Tyr Lys Asp Val Ile Leu Trp Phe Ser Phe 
            500                 505                 510         


Gly Ala Ser Cys Phe Ile Leu Leu Ala Ile Val Met Gly Leu Val Phe 
        515                 520                 525             


Ile Cys Val Lys Asn Gly Asn Met Arg Cys Thr Ile Cys Ile 
    530                 535                 540         


<210>  207
<211>  569
<212>  PRT
<213>  Influenza Virus

<400>  207

Asp Arg Ile Cys Thr Gly Ile Thr Ser Ser Asn Ser Pro His Val Val 
1               5                   10                  15      


Lys Thr Ala Thr Gln Gly Glu Val Asn Val Thr Gly Val Ile Pro Leu 
            20                  25                  30          


Thr Thr Thr Pro Thr Lys Ser Tyr Phe Ala Asn Leu Lys Gly Thr Arg 
        35                  40                  45              


Thr Arg Gly Lys Leu Cys Pro Asp Cys Leu Asn Cys Thr Asp Leu Asp 
    50                  55                  60                  


Val Ala Leu Gly Arg Pro Met Cys Val Gly Thr Thr Pro Ser Ala Lys 
65                  70                  75                  80  


Ala Ser Ile Leu His Glu Val Arg Pro Val Thr Ser Gly Cys Phe Pro 
                85                  90                  95      


Ile Met His Asp Arg Thr Lys Ile Arg Gln Leu Pro Asn Leu Leu Arg 
            100                 105                 110         


Gly Tyr Glu Lys Ile Arg Leu Ser Thr Gln Asn Val Ile Asp Ala Glu 
        115                 120                 125             


Lys Ala Pro Gly Gly Pro Tyr Arg Leu Gly Thr Ser Gly Ser Cys Pro 
    130                 135                 140                 


Asn Ala Thr Ser Lys Ile Gly Phe Phe Ala Thr Met Ala Trp Ala Val 
145                 150                 155                 160 


Pro Lys Asp Asn Tyr Lys Asn Ala Thr Asn Pro Leu Thr Val Glu Val 
                165                 170                 175     


Pro Tyr Ile Cys Thr Glu Gly Glu Asp Gln Ile Thr Val Trp Gly Phe 
            180                 185                 190         


His Ser Asp Asn Lys Thr Gln Met Lys Ser Leu Tyr Gly Asp Ser Asn 
        195                 200                 205             


Pro Gln Lys Phe Thr Ser Ser Ala Asn Gly Val Thr Thr His Tyr Val 
    210                 215                 220                 


Ser Gln Ile Gly Asp Phe Pro Asp Gln Thr Glu Asp Gly Gly Leu Pro 
225                 230                 235                 240 


Gln Ser Gly Arg Ile Val Val Asp Tyr Met Met Gln Lys Pro Gly Lys 
                245                 250                 255     


Thr Gly Thr Ile Val Tyr Gln Arg Gly Val Leu Leu Pro Gln Lys Val 
            260                 265                 270         


Trp Cys Ala Ser Gly Arg Ser Lys Val Ile Lys Gly Ser Leu Pro Leu 
        275                 280                 285             


Ile Gly Glu Ala Asp Cys Leu His Glu Glu Tyr Gly Gly Leu Asn Lys 
    290                 295                 300                 


Ser Lys Pro Tyr Tyr Thr Gly Lys His Ala Lys Ala Ile Gly Asn Cys 
305                 310                 315                 320 


Pro Ile Trp Val Lys Thr Pro Leu Lys Leu Ala Asn Gly Thr Lys Tyr 
                325                 330                 335     


Arg Pro Pro Ala Lys Leu Leu Lys Glu Arg Gly Phe Phe Gly Ala Ile 
            340                 345                 350         


Ala Gly Phe Leu Glu Gly Gly Trp Glu Gly Met Ile Ala Gly Trp His 
        355                 360                 365             


Gly Tyr Thr Ser His Gly Ala His Gly Val Ala Val Ala Ala Asp Leu 
    370                 375                 380                 


Lys Ser Thr Gln Glu Ala Ile Asn Lys Ile Thr Lys Asn Leu Asn Ser 
385                 390                 395                 400 


Leu Ser Glu Leu Glu Val Lys Asn Leu Gln Arg Leu Ser Gly Ala Met 
                405                 410                 415     


Asp Glu Leu His Asn Glu Ile Leu Glu Leu Asp Glu Lys Val Asp Asp 
            420                 425                 430         


Leu Arg Ala Asp Thr Ile Ser Ser Gln Ile Glu Leu Ala Val Leu Leu 
        435                 440                 445             


Ser Asn Glu Gly Ile Ile Asn Ser Glu Asp Glu His Leu Leu Ala Leu 
    450                 455                 460                 


Glu Arg Lys Leu Lys Lys Met Leu Gly Pro Ser Ala Val Asp Ile Gly 
465                 470                 475                 480 


Asn Gly Cys Phe Glu Thr Lys His Lys Cys Asn Gln Thr Cys Leu Asp 
                485                 490                 495     


Arg Ile Ala Ala Gly Thr Phe Asn Ala Gly Glu Phe Ser Leu Pro Thr 
            500                 505                 510         


Phe Asp Ser Leu Asn Ile Thr Ala Ala Ser Leu Asn Asp Asp Gly Leu 
        515                 520                 525             


Asp Asn His Thr Ile Leu Leu Tyr Tyr Ser Thr Ala Ala Ser Ser Leu 
    530                 535                 540                 


Ala Val Thr Leu Met Leu Ala Ile Phe Ile Val Tyr Met Val Ser Arg 
545                 550                 555                 560 


Asp Asn Val Ser Cys Ser Ile Cys Leu 
                565                 


<210>  208
<211>  568
<212>  PRT
<213>  Influenza Virus

<400>  208

Asp Arg Ile Cys Thr Gly Ile Thr Ser Ser Asn Ser Pro His Val Val 
1               5                   10                  15      


Lys Thr Ala Thr Gln Gly Glu Val Asn Val Thr Gly Val Ile Pro Leu 
            20                  25                  30          


Thr Thr Thr Pro Thr Lys Ser His Phe Ala Asn Leu Lys Gly Thr Glu 
        35                  40                  45              


Thr Arg Gly Lys Leu Cys Pro Lys Cys Leu Asn Cys Thr Asp Leu Asp 
    50                  55                  60                  


Val Ala Leu Gly Arg Pro Lys Cys Thr Gly Lys Ile Pro Ser Ala Arg 
65                  70                  75                  80  


Val Ser Ile Leu His Glu Val Arg Pro Val Thr Ser Gly Cys Phe Pro 
                85                  90                  95      


Ile Met His Asp Arg Thr Lys Ile Arg Gln Leu Pro Asn Leu Leu Arg 
            100                 105                 110         


Gly Tyr Glu His Val Arg Leu Ser Thr His Asn Val Ile Asn Ala Glu 
        115                 120                 125             


Asp Ala Pro Gly Gly Pro Tyr Lys Ile Gly Thr Ser Gly Ser Cys Pro 
    130                 135                 140                 


Asn Ile Thr Asn Gly Asn Gly Phe Phe Ala Thr Met Ala Trp Ala Val 
145                 150                 155                 160 


Pro Asp Lys Asn Lys Thr Ala Thr Asn Pro Leu Thr Ile Glu Val Pro 
                165                 170                 175     


Tyr Val Cys Thr Glu Gly Glu Asp Gln Ile Thr Val Trp Gly Phe His 
            180                 185                 190         


Ser Asp Asn Glu Thr Gln Met Ala Lys Leu Tyr Gly Asp Ser Lys Pro 
        195                 200                 205             


Gln Lys Phe Thr Ser Ser Ala Asn Gly Val Thr Thr His Tyr Val Ser 
    210                 215                 220                 


Gln Ile Gly Gly Phe Pro Asn Gln Thr Glu Asp Gly Gly Leu Pro Gln 
225                 230                 235                 240 


Ser Gly Arg Ile Val Val Asp Tyr Met Val Gln Lys Ser Gly Lys Thr 
                245                 250                 255     


Gly Thr Ile Thr Tyr Gln Arg Gly Ile Leu Leu Pro Gln Lys Val Trp 
            260                 265                 270         


Cys Ala Ser Gly Arg Ser Lys Val Ile Lys Gly Ser Leu Pro Leu Ile 
        275                 280                 285             


Gly Glu Ala Asp Cys Leu His Glu Lys Tyr Gly Gly Leu Asn Lys Ser 
    290                 295                 300                 


Lys Pro Tyr Tyr Thr Gly Glu His Ala Lys Ala Ile Gly Asn Cys Pro 
305                 310                 315                 320 


Ile Trp Val Lys Thr Pro Leu Lys Leu Ala Asn Gly Thr Lys Tyr Arg 
                325                 330                 335     


Pro Pro Ala Lys Leu Leu Lys Glu Arg Gly Phe Phe Gly Ala Ile Ala 
            340                 345                 350         


Gly Phe Leu Glu Gly Gly Trp Glu Gly Met Ile Ala Gly Trp His Gly 
        355                 360                 365             


Tyr Thr Ser His Gly Ala His Gly Val Ala Val Ala Ala Asp Leu Lys 
    370                 375                 380                 


Ser Thr Gln Glu Ala Ile Asn Lys Ile Thr Lys Asn Leu Asn Ser Leu 
385                 390                 395                 400 


Ser Glu Leu Glu Val Lys Asn Leu Gln Arg Leu Ser Gly Ala Met Asp 
                405                 410                 415     


Glu Leu His Asn Glu Ile Leu Glu Leu Asp Glu Lys Val Asp Asp Leu 
            420                 425                 430         


Arg Ala Asp Thr Ile Ser Ser Gln Ile Glu Leu Ala Val Leu Leu Ser 
        435                 440                 445             


Asn Glu Gly Ile Ile Asn Ser Glu Asp Glu His Leu Leu Ala Leu Glu 
    450                 455                 460                 


Arg Lys Leu Lys Lys Met Leu Gly Pro Ser Ala Val Glu Ile Gly Asn 
465                 470                 475                 480 


Gly Cys Phe Glu Thr Lys His Lys Cys Asn Gln Thr Cys Leu Asp Lys 
                485                 490                 495     


Ile Ala Ala Gly Thr Phe Asp Ala Gly Glu Phe Ser Leu Pro Thr Phe 
            500                 505                 510         


Asp Ser Leu Asn Ile Thr Ala Ala Ser Leu Asn Asp Asp Gly Leu Asp 
        515                 520                 525             


Asn His Thr Ile Leu Leu Tyr Tyr Ser Thr Ala Ala Ser Ser Leu Ala 
    530                 535                 540                 


Val Thr Leu Met Ile Ala Ile Phe Val Val Tyr Met Val Ser Arg Asp 
545                 550                 555                 560 


Asn Val Ser Cys Ser Ile Cys Leu 
                565             


<210>  209
<211>  567
<212>  PRT
<213>  Influenza Virus

<400>  209

Asp Arg Ile Cys Thr Gly Ile Thr Ser Ser Asn Ser Pro His Val Val 
1               5                   10                  15      


Lys Thr Ala Thr Gln Gly Glu Val Asn Val Thr Gly Val Ile Pro Leu 
            20                  25                  30          


Thr Thr Thr Pro Thr Lys Ser His Phe Ala Asn Leu Lys Gly Thr Glu 
        35                  40                  45              


Thr Arg Gly Lys Leu Cys Pro Lys Cys Leu Asn Cys Thr Asp Leu Asp 
    50                  55                  60                  


Val Ala Leu Ser Arg Pro Lys Cys Thr Gly Lys Ile Pro Ser Ala Arg 
65                  70                  75                  80  


Val Ser Ile Leu His Glu Val Arg Pro Val Thr Ser Gly Cys Phe Pro 
                85                  90                  95      


Ile Met His Asp Arg Thr Lys Ile Arg Gln Leu Pro Asn Leu Leu Arg 
            100                 105                 110         


Gly Tyr Glu His Val Arg Leu Ser Thr His Asn Val Ile Asn Ala Glu 
        115                 120                 125             


Asp Ala Pro Gly Gly Pro Tyr Glu Ile Gly Thr Ser Gly Ser Cys Pro 
    130                 135                 140                 


Asn Ile Thr Asn Gly Asn Gly Phe Phe Ala Thr Met Ala Trp Ala Val 
145                 150                 155                 160 


Pro Lys Asn Lys Thr Ala Thr Asn Pro Leu Thr Ile Glu Val Pro Tyr 
                165                 170                 175     


Ile Cys Thr Glu Gly Glu Asp Gln Ile Thr Val Trp Gly Phe His Ser 
            180                 185                 190         


Asp Asn Glu Thr Gln Met Ala Lys Leu Tyr Gly Asp Ser Lys Pro Gln 
        195                 200                 205             


Lys Phe Thr Ser Ser Ala Asn Gly Val Thr Thr His Tyr Val Ser Gln 
    210                 215                 220                 


Ile Gly Gly Phe Pro Asn Gln Thr Glu Asp Gly Gly Leu Pro Gln Ser 
225                 230                 235                 240 


Gly Arg Ile Val Val Asp Tyr Met Val Gln Lys Ser Gly Lys Thr Gly 
                245                 250                 255     


Thr Ile Thr Tyr Gln Arg Gly Ile Leu Leu Pro Gln Lys Val Trp Cys 
            260                 265                 270         


Ala Ser Gly Arg Ser Lys Val Ile Lys Gly Ser Leu Pro Leu Ile Gly 
        275                 280                 285             


Glu Ala Asp Cys Leu His Glu Lys Tyr Gly Gly Leu Asn Lys Ser Lys 
    290                 295                 300                 


Pro Tyr Tyr Thr Gly Glu His Ala Lys Ala Ile Gly Asn Cys Pro Ile 
305                 310                 315                 320 


Trp Val Lys Thr Pro Leu Lys Leu Ala Asn Gly Thr Lys Tyr Arg Pro 
                325                 330                 335     


Pro Ala Lys Leu Leu Lys Glu Arg Gly Phe Phe Gly Ala Ile Ala Gly 
            340                 345                 350         


Phe Leu Glu Gly Gly Trp Glu Gly Met Ile Ala Gly Trp His Gly Tyr 
        355                 360                 365             


Thr Ser His Gly Ala His Gly Val Ala Val Ala Ala Asp Leu Lys Ser 
    370                 375                 380                 


Thr Gln Glu Ala Ile Asn Lys Ile Thr Lys Asn Leu Asn Ser Leu Ser 
385                 390                 395                 400 


Glu Leu Glu Val Lys Asn Leu Gln Arg Leu Ser Gly Ala Met Asp Glu 
                405                 410                 415     


Leu His Asn Glu Ile Leu Glu Leu Asp Glu Lys Val Asp Asp Leu Arg 
            420                 425                 430         


Ala Asp Thr Ile Ser Ser Gln Ile Glu Leu Ala Val Leu Leu Ser Asn 
        435                 440                 445             


Glu Gly Ile Ile Asn Ser Glu Asp Glu His Leu Leu Ala Leu Glu Arg 
    450                 455                 460                 


Lys Leu Lys Lys Met Leu Gly Pro Ser Ala Val Glu Ile Gly Asn Gly 
465                 470                 475                 480 


Cys Phe Glu Thr Lys His Lys Cys Asn Gln Thr Cys Leu Asp Arg Ile 
                485                 490                 495     


Ala Ala Gly Thr Phe Asp Ala Gly Glu Phe Ser Leu Pro Thr Phe Asp 
            500                 505                 510         


Ser Leu Asn Ile Thr Ala Ala Ser Leu Asn Asp Asp Gly Leu Asp Asn 
        515                 520                 525             


His Thr Ile Leu Leu Tyr Tyr Ser Thr Ala Ala Ser Ser Leu Ala Val 
    530                 535                 540                 


Thr Leu Met Ile Ala Ile Phe Val Val Tyr Met Val Ser Arg Asp Ser 
545                 550                 555                 560 


Val Ser Cys Ser Ile Cys Leu 
                565         


