                         SEQUENCE LISTING

<110>  bluebird bio, Inc.
       Evans, John W.
 
<120>  MODIFIED CCR POLYPEPTIDES AND USES THEREOF

<130>  BLUE-127.PC

<150>  US 63/011,494
<151>  2020-04-17

<160>  61    

<170>  PatentIn version 3.5

<210>  1
<211>  235
<212>  PRT
<213>  Homo sapiens

<400>  1

Met Ala Leu Pro Val Thr Ala Leu Leu Leu Pro Leu Ala Leu Leu Leu 
1               5                   10                  15      


His Ala Ala Arg Pro Ser Gln Phe Arg Val Ser Pro Leu Asp Arg Thr 
            20                  25                  30          


Trp Asn Leu Gly Glu Thr Val Glu Leu Lys Cys Gln Val Leu Leu Ser 
        35                  40                  45              


Asn Pro Thr Ser Gly Cys Ser Trp Leu Phe Gln Pro Arg Gly Ala Ala 
    50                  55                  60                  


Ala Ser Pro Thr Phe Leu Leu Tyr Leu Ser Gln Asn Lys Pro Lys Ala 
65                  70                  75                  80  


Ala Glu Gly Leu Asp Thr Gln Arg Phe Ser Gly Lys Arg Leu Gly Asp 
                85                  90                  95      


Thr Phe Val Leu Thr Leu Ser Asp Phe Arg Arg Glu Asn Glu Gly Tyr 
            100                 105                 110         


Tyr Phe Cys Ser Ala Leu Ser Asn Ser Ile Met Tyr Phe Ser His Phe 
        115                 120                 125             


Val Pro Val Phe Leu Pro Ala Lys Pro Thr Thr Thr Pro Ala Pro Arg 
    130                 135                 140                 


Pro Pro Thr Pro Ala Pro Thr Ile Ala Ser Gln Pro Leu Ser Leu Arg 
145                 150                 155                 160 


Pro Glu Ala Cys Arg Pro Ala Ala Gly Gly Ala Val His Thr Arg Gly 
                165                 170                 175     


Leu Asp Phe Ala Cys Asp Ile Tyr Ile Trp Ala Pro Leu Ala Gly Thr 
            180                 185                 190         


Cys Gly Val Leu Leu Leu Ser Leu Val Ile Thr Leu Tyr Cys Asn His 
        195                 200                 205             


Arg Asn Arg Arg Arg Val Cys Lys Cys Pro Arg Pro Val Val Lys Ser 
    210                 215                 220                 


Gly Asp Lys Pro Ser Leu Ser Ala Arg Tyr Val 
225                 230                 235 


<210>  2
<211>  45
<212>  PRT
<213>  Homo sapiens

<400>  2

Thr Thr Thr Pro Ala Pro Arg Pro Pro Thr Pro Ala Pro Thr Ile Ala 
1               5                   10                  15      


Ser Gln Pro Leu Ser Leu Arg Pro Glu Ala Cys Arg Pro Ala Ala Gly 
            20                  25                  30          


Gly Ala Val His Thr Arg Gly Leu Asp Phe Ala Cys Asp 
        35                  40                  45  


<210>  3
<211>  45
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Made in Lab - Modified CD8alpha hinge

<400>  3

Thr Thr Thr Pro Ala Pro Arg Pro Pro Thr Pro Ala Pro Thr Ile Ala 
1               5                   10                  15      


Ser Gln Pro Leu Ser Leu Arg Pro Glu Ala Ser Arg Pro Ala Ala Gly 
            20                  25                  30          


Gly Ala Val His Thr Arg Gly Leu Asp Phe Ala Cys Asp 
        35                  40                  45  


<210>  4
<211>  45
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Made in Lab - Modified CD8alpha hinge

<400>  4

Thr Thr Thr Pro Ala Pro Arg Pro Pro Thr Pro Ala Pro Thr Ile Ala 
1               5                   10                  15      


Ser Gln Pro Leu Ser Leu Arg Pro Glu Ala Cys Arg Pro Ala Ala Gly 
            20                  25                  30          


Gly Ala Val His Thr Arg Gly Leu Asp Phe Ala Ser Asp 
        35                  40                  45  


<210>  5
<211>  45
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Made in Lab - Modified CD8alpha hinge

<400>  5

Thr Thr Thr Pro Ala Pro Arg Pro Pro Thr Pro Ala Pro Thr Ile Ala 
1               5                   10                  15      


Ser Gln Pro Leu Ser Leu Arg Pro Glu Ala Ser Arg Pro Ala Ala Gly 
            20                  25                  30          


Gly Ala Val His Thr Arg Gly Leu Asp Phe Ala Ser Asp 
        35                  40                  45  


<210>  6
<211>  21
<212>  PRT
<213>  Homo sapiens

<400>  6

Ile Tyr Ile Trp Ala Pro Leu Ala Gly Thr Cys Gly Val Leu Leu Leu 
1               5                   10                  15      


Ser Leu Val Ile Thr 
            20      


<210>  7
<211>  22
<212>  PRT
<213>  Homo sapiens

<400>  7

Ser Gly Gln Val Leu Leu Glu Ser Asn Ile Lys Val Leu Pro Thr Trp 
1               5                   10                  15      


Ser Thr Pro Val Gln Pro 
            20          


<210>  8
<211>  15
<212>  PRT
<213>  Homo sapiens

<400>  8

Lys His Leu Cys Pro Ser Pro Leu Phe Pro Gly Pro Ser Lys Pro 
1               5                   10                  15  


<210>  9
<211>  36
<212>  PRT
<213>  Homo sapiens

<400>  9

Ala Pro Pro Arg Ala Ser Ala Leu Pro Ala Pro Pro Thr Gly Ser Ala 
1               5                   10                  15      


Leu Pro Asp Pro Gln Thr Ala Ser Ala Leu Pro Asp Pro Pro Ala Ala 
            20                  25                  30          


Ser Ala Leu Pro 
        35      


<210>  10
<211>  39
<212>  PRT
<213>  Homo sapiens

<400>  10

Asp Thr Gly Leu Tyr Ile Cys Lys Val Glu Leu Met Tyr Pro Pro Pro 
1               5                   10                  15      


Tyr Tyr Leu Gly Ile Gly Asn Gly Thr Gln Ile Tyr Val Ile Asp Pro 
            20                  25                  30          


Glu Pro Cys Pro Asp Ser Asp 
        35                  


<210>  11
<211>  38
<212>  PRT
<213>  Homo sapiens

<400>  11

Gln Ile Lys Glu Ser Leu Arg Ala Glu Leu Arg Val Thr Glu Arg Arg 
1               5                   10                  15      


Ala Glu Val Pro Thr Ala His Pro Ser Pro Ser Pro Arg Pro Ala Gly 
            20                  25                  30          


Gln Phe Gln Thr Leu Val 
        35              


<210>  12
<211>  15
<212>  PRT
<213>  Homo sapiens

<400>  12

Glu Pro Lys Ser Cys Asp Lys Thr His Thr Cys Pro Pro Cys Pro 
1               5                   10                  15  


<210>  13
<211>  12
<212>  PRT
<213>  Homo sapiens

<400>  13

Glu Arg Lys Cys Cys Val Glu Cys Pro Pro Cys Pro 
1               5                   10          


<210>  14
<211>  62
<212>  PRT
<213>  Homo sapiens

<400>  14

Glu Leu Lys Thr Pro Leu Gly Asp Thr Thr His Thr Cys Pro Arg Cys 
1               5                   10                  15      


Pro Glu Pro Lys Ser Cys Asp Thr Pro Pro Pro Cys Pro Arg Cys Pro 
            20                  25                  30          


Glu Pro Lys Ser Cys Asp Thr Pro Pro Pro Cys Pro Arg Cys Pro Glu 
        35                  40                  45              


Pro Lys Ser Cys Asp Thr Pro Pro Pro Cys Pro Arg Cys Pro 
    50                  55                  60          


<210>  15
<211>  12
<212>  PRT
<213>  Homo sapiens

<400>  15

Glu Ser Lys Tyr Gly Pro Pro Cys Pro Ser Cys Pro 
1               5                   10          


<210>  16
<211>  231
<212>  PRT
<213>  Homo sapiens

<400>  16

Glu Pro Lys Ser Cys Asp Lys Thr His Thr Cys Pro Pro Cys Pro Ala 
1               5                   10                  15      


Pro Pro Val Ala Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys 
            20                  25                  30          


Asp Thr Leu Met Ile Ala Arg Thr Pro Glu Val Thr Cys Val Val Val 
        35                  40                  45              


Asp Val Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr Val Asp 
    50                  55                  60                  


Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr 
65                  70                  75                  80  


Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp 
                85                  90                  95      


Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu 
            100                 105                 110         


Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg 
        115                 120                 125             


Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Asp Glu Leu Thr Lys 
    130                 135                 140                 


Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp 
145                 150                 155                 160 


Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys 
                165                 170                 175     


Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser 
            180                 185                 190         


Lys Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser 
        195                 200                 205             


Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser 
    210                 215                 220                 


Leu Ser Leu Ser Pro Gly Lys 
225                 230     


<210>  17
<211>  140
<212>  PRT
<213>  Homo sapiens

<400>  17

Glu Pro Lys Ser Cys Asp Lys Thr His Thr Cys Pro Pro Cys Pro Gly 
1               5                   10                  15      


Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Asp Glu 
            20                  25                  30          


Leu Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr 
        35                  40                  45              


Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn 
    50                  55                  60                  


Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe 
65                  70                  75                  80  


Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn 
                85                  90                  95      


Val Phe Ser Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr 
            100                 105                 110         


Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys Gly Leu Gln Leu Asp Glu 
        115                 120                 125             


Thr Cys Ala Glu Ala Gln Asp Gly Glu Leu Asp Gly 
    130                 135                 140 


<210>  18
<211>  229
<212>  PRT
<213>  Homo sapiens

<400>  18

Glu Ser Lys Tyr Gly Pro Pro Cys Pro Ser Cys Pro Ala Pro Glu Phe 
1               5                   10                  15      


Glu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr 
            20                  25                  30          


Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val 
        35                  40                  45              


Ser Gln Glu Asp Pro Glu Val Gln Phe Asn Trp Tyr Val Asp Gly Val 
    50                  55                  60                  


Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Phe Gln Ser 
65                  70                  75                  80  


Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu 
                85                  90                  95      


Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Gly Leu Pro Ser 
            100                 105                 110         


Ser Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro 
        115                 120                 125             


Gln Val Tyr Thr Leu Pro Pro Ser Gln Glu Glu Met Thr Lys Asn Gln 
    130                 135                 140                 


Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala 
145                 150                 155                 160 


Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr 
                165                 170                 175     


Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Arg Leu 
            180                 185                 190         


Thr Val Asp Lys Ser Arg Trp Gln Glu Gly Asn Val Phe Ser Cys Ser 
        195                 200                 205             


Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser 
    210                 215                 220                 


Leu Ser Leu Gly Lys 
225                 


<210>  19
<211>  229
<212>  PRT
<213>  Homo sapiens

<400>  19

Glu Ser Lys Tyr Gly Pro Pro Cys Pro Ser Cys Pro Ala Pro Glu Phe 
1               5                   10                  15      


Glu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr 
            20                  25                  30          


Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val 
        35                  40                  45              


Ser Gln Glu Asp Pro Glu Val Gln Phe Asn Trp Tyr Val Asp Gly Val 
    50                  55                  60                  


Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Phe Gln Ser 
65                  70                  75                  80  


Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu 
                85                  90                  95      


Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Gly Leu Pro Ser 
            100                 105                 110         


Ser Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro 
        115                 120                 125             


Gln Val Tyr Thr Leu Pro Pro Ser Gln Glu Glu Met Thr Lys Asn Gln 
    130                 135                 140                 


Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala 
145                 150                 155                 160 


Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr 
                165                 170                 175     


Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Arg Leu 
            180                 185                 190         


Thr Val Asp Lys Ser Arg Trp Gln Glu Gly Asn Val Phe Ser Cys Ser 
        195                 200                 205             


Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser 
    210                 215                 220                 


Leu Ser Leu Gly Lys 
225                 


<210>  20
<211>  53
<212>  PRT
<213>  Homo sapiens

<400>  20

Ser Gly Thr Tyr Leu Cys Gly Ala Ile Ser Leu Ala Pro Lys Ala Gln 
1               5                   10                  15      


Ile Lys Glu Ser Leu Arg Ala Glu Leu Arg Val Thr Glu Arg Arg Ala 
            20                  25                  30          


Glu Val Pro Thr Ala His Pro Ser Pro Ser Pro Arg Pro Ala Gly Gln 
        35                  40                  45              


Phe Gln Thr Leu Val 
    50              


<210>  21
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Peptide linker sequence

<400>  21

Asp Gly Gly Gly Ser 
1               5   


<210>  22
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Peptide linker sequence

<400>  22

Thr Gly Glu Lys Pro 
1               5   


<210>  23
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Peptide linker sequence

<400>  23

Gly Gly Arg Arg 
1               


<210>  24
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Peptide linker sequence

<400>  24

Gly Gly Gly Gly Ser 
1               5   


<210>  25
<211>  14
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Peptide linker sequence

<400>  25

Glu Gly Lys Ser Ser Gly Ser Gly Ser Glu Ser Lys Val Asp 
1               5                   10                  


<210>  26
<211>  18
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Peptide linker sequence

<400>  26

Lys Glu Ser Gly Ser Val Ser Ser Glu Gln Leu Ala Gln Phe Arg Ser 
1               5                   10                  15      


Leu Asp 
        


<210>  27
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Peptide linker sequence

<400>  27

Gly Gly Arg Arg Gly Gly Gly Ser 
1               5               


<210>  28
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Peptide linker sequence

<400>  28

Leu Arg Gln Arg Asp Gly Glu Arg Pro 
1               5                   


<210>  29
<211>  12
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Peptide linker sequence

<400>  29

Leu Arg Gln Lys Asp Gly Gly Gly Ser Glu Arg Pro 
1               5                   10          


<210>  30
<211>  16
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Peptide linker sequence

<400>  30

Leu Arg Gln Lys Asp Gly Gly Gly Ser Gly Gly Gly Ser Glu Arg Pro 
1               5                   10                  15      


<210>  31
<211>  18
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Peptide linker sequence

<400>  31

Gly Ser Thr Ser Gly Ser Gly Lys Pro Gly Ser Gly Glu Gly Ser Thr 
1               5                   10                  15      


Lys Gly 
        


<210>  32
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cleavage sequence by TEV protease


<220>
<221>  misc_feature
<222>  (2)..(3)
<223>  Xaa is any amino acid

<220>
<221>  misc_feature
<222>  (5)..(5)
<223>  Xaa is any amino acid

<220>
<221>  MISC_FEATURE
<222>  (7)..(7)
<223>  Xaa = Gly or Ser

<400>  32

Glu Xaa Xaa Tyr Xaa Gln Xaa 
1               5           


<210>  33
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cleavage sequence by TEV protease

<400>  33

Glu Asn Leu Tyr Phe Gln Gly 
1               5           


<210>  34
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cleavage sequence by TEV protease

<400>  34

Glu Asn Leu Tyr Phe Gln Ser 
1               5           


<210>  35
<211>  22
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Self-cleaving polypeptide comprising 2A site

<400>  35

Gly Ser Gly Ala Thr Asn Phe Ser Leu Leu Lys Gln Ala Gly Asp Val 
1               5                   10                  15      


Glu Glu Asn Pro Gly Pro 
            20          


<210>  36
<211>  19
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Self-cleaving polypeptide comprising 2A site

<400>  36

Ala Thr Asn Phe Ser Leu Leu Lys Gln Ala Gly Asp Val Glu Glu Asn 
1               5                   10                  15      


Pro Gly Pro 
            


<210>  37
<211>  14
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Self-cleaving polypeptide comprising 2A site

<400>  37

Leu Leu Lys Gln Ala Gly Asp Val Glu Glu Asn Pro Gly Pro 
1               5                   10                  


<210>  38
<211>  21
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Self-cleaving polypeptide comprising 2A site

<400>  38

Gly Ser Gly Glu Gly Arg Gly Ser Leu Leu Thr Cys Gly Asp Val Glu 
1               5                   10                  15      


Glu Asn Pro Gly Pro 
            20      


<210>  39
<211>  18
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Self-cleaving polypeptide comprising 2A site

<400>  39

Glu Gly Arg Gly Ser Leu Leu Thr Cys Gly Asp Val Glu Glu Asn Pro 
1               5                   10                  15      


Gly Pro 
        


<210>  40
<211>  13
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Self-cleaving polypeptide comprising 2A site

<400>  40

Leu Leu Thr Cys Gly Asp Val Glu Glu Asn Pro Gly Pro 
1               5                   10              


<210>  41
<211>  23
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Self-cleaving polypeptide comprising 2A site

<400>  41

Gly Ser Gly Gln Cys Thr Asn Tyr Ala Leu Leu Lys Leu Ala Gly Asp 
1               5                   10                  15      


Val Glu Ser Asn Pro Gly Pro 
            20              


<210>  42
<211>  20
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Self-cleaving polypeptide comprising 2A site

<400>  42

Gln Cys Thr Asn Tyr Ala Leu Leu Lys Leu Ala Gly Asp Val Glu Ser 
1               5                   10                  15      


Asn Pro Gly Pro 
            20  


<210>  43
<211>  14
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Self-cleaving polypeptide comprising 2A site

<400>  43

Leu Leu Lys Leu Ala Gly Asp Val Glu Ser Asn Pro Gly Pro 
1               5                   10                  


<210>  44
<211>  25
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Self-cleaving polypeptide comprising 2A site

<400>  44

Gly Ser Gly Val Lys Gln Thr Leu Asn Phe Asp Leu Leu Lys Leu Ala 
1               5                   10                  15      


Gly Asp Val Glu Ser Asn Pro Gly Pro 
            20                  25  


<210>  45
<211>  22
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Self-cleaving polypeptide comprising 2A site

<400>  45

Val Lys Gln Thr Leu Asn Phe Asp Leu Leu Lys Leu Ala Gly Asp Val 
1               5                   10                  15      


Glu Ser Asn Pro Gly Pro 
            20          


<210>  46
<211>  14
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Self-cleaving polypeptide comprising 2A site

<400>  46

Leu Leu Lys Leu Ala Gly Asp Val Glu Ser Asn Pro Gly Pro 
1               5                   10                  


<210>  47
<211>  19
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Self-cleaving polypeptide comprising 2A site

<400>  47

Leu Leu Asn Phe Asp Leu Leu Lys Leu Ala Gly Asp Val Glu Ser Asn 
1               5                   10                  15      


Pro Gly Pro 
            


<210>  48
<211>  19
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Self-cleaving polypeptide comprising 2A site

<400>  48

Thr Leu Asn Phe Asp Leu Leu Lys Leu Ala Gly Asp Val Glu Ser Asn 
1               5                   10                  15      


Pro Gly Pro 
            


<210>  49
<211>  14
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Self-cleaving polypeptide comprising 2A site

<400>  49

Leu Leu Lys Leu Ala Gly Asp Val Glu Ser Asn Pro Gly Pro 
1               5                   10                  


<210>  50
<211>  17
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Self-cleaving polypeptide comprising 2A site

<400>  50

Asn Phe Asp Leu Leu Lys Leu Ala Gly Asp Val Glu Ser Asn Pro Gly 
1               5                   10                  15      


Pro 
    


<210>  51
<211>  20
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Self-cleaving polypeptide comprising 2A site

<400>  51

Gln Leu Leu Asn Phe Asp Leu Leu Lys Leu Ala Gly Asp Val Glu Ser 
1               5                   10                  15      


Asn Pro Gly Pro 
            20  


<210>  52
<211>  24
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Self-cleaving polypeptide comprising 2A site

<400>  52

Ala Pro Val Lys Gln Thr Leu Asn Phe Asp Leu Leu Lys Leu Ala Gly 
1               5                   10                  15      


Asp Val Glu Ser Asn Pro Gly Pro 
            20                  


<210>  53
<211>  40
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Self-cleaving polypeptide comprising 2A site

<400>  53

Val Thr Glu Leu Leu Tyr Arg Met Lys Arg Ala Glu Thr Tyr Cys Pro 
1               5                   10                  15      


Arg Pro Leu Leu Ala Ile His Pro Thr Glu Ala Arg His Lys Gln Lys 
            20                  25                  30          


Ile Val Ala Pro Val Lys Gln Thr 
        35                  40  


<210>  54
<211>  18
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Self-cleaving polypeptide comprising 2A site

<400>  54

Leu Asn Phe Asp Leu Leu Lys Leu Ala Gly Asp Val Glu Ser Asn Pro 
1               5                   10                  15      


Gly Pro 
        


<210>  55
<211>  40
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Self-cleaving polypeptide comprising 2A site

<400>  55

Leu Leu Ala Ile His Pro Thr Glu Ala Arg His Lys Gln Lys Ile Val 
1               5                   10                  15      


Ala Pro Val Lys Gln Thr Leu Asn Phe Asp Leu Leu Lys Leu Ala Gly 
            20                  25                  30          


Asp Val Glu Ser Asn Pro Gly Pro 
        35                  40  


<210>  56
<211>  33
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Self-cleaving polypeptide comprising 2A site

<400>  56

Glu Ala Arg His Lys Gln Lys Ile Val Ala Pro Val Lys Gln Thr Leu 
1               5                   10                  15      


Asn Phe Asp Leu Leu Lys Leu Ala Gly Asp Val Glu Ser Asn Pro Gly 
            20                  25                  30          


Pro 
    


<210>  57
<211>  10
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Consensus Kozak sequence

<400>  57
gccrccatgg                                                              10


<210>  58
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Exemplary rule for determining light chain CDR-L3 motif


<220>
<221>  MOD_RES
<222>  (3)..(3)
<223>  Xaa is any amino acid

<400>  58

Phe Gly Xaa Gly 
1               


<210>  59
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Exemplary rule for determining heavy chain CDR-H1 motif


<220>
<221>  MOD_RES
<222>  (2)..(4)
<223>  Xaa is any amino acid

<400>  59

Cys Xaa Xaa Xaa 
1               


<210>  60
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Exemplary rule for determining heavy chain CDR-H2 motif

<400>  60

Leu Glu Trp Ile Gly 
1               5   


<210>  61
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Exemplary rule for determining heavy chain CDR-H3 motif


<220>
<221>  MOD_RES
<222>  (3)..(3)
<223>  Xaa is any amino acd

<400>  61

Trp Gly Xaa Gly 
1               


